KR20040024209A - Cosmetic composition containing octacosanol - Google Patents

Abstract

PURPOSE: Provided is a cosmetic composition which contains octacosanol, and exerts anti-aging effect by moisturizing the skin, giving elasticity thereto and removing wrinkles thereof. CONSTITUTION: The cosmetic composition comprises 0.01-5wt.% of octacosanol. The octacosanol content is added to a skin lotion, a nourishing lotion, a nourishing cream, an essence, or a massage cream. When the amount of octacosanol is less than 0.01wt.%, the desired anti-aging effect is not exerted. By contrast, when the amount of octacosanol exceeds 5wt.%, the production efficiency is deteriorated.

Description

Cosmetic composition containing octacosanol {COSMETIC COMPOSITION CONTAINING OCTACOSANOL}

The present invention relates to a cosmetic composition containing octacosanol, and more particularly to an anti-aging cosmetic composition containing octacosanol.

Up to now cosmetics have been used as a tool to beautify women or clean the skin, but cosmetics that give physiological activity to the skin are increasingly required, and in particular, attention has been focused on anti-aging and skin wrinkle control cosmetics.

Although physiological progression of aging cannot be prevented at the source, studies that slowly slow the progress of skin aging are ongoing.

In this regard, studies have been conducted to smooth out collagen metabolism to suppress skin wrinkles. Decreased collagen metabolism promotes aging of skin tissue. Specifically, when collagenase, which facilitates collagen metabolism, is degraded, collagen production is not controlled and the skin ages. Therefore, cosmetics containing substances that increase the activity of collagenase, such as ammonium acetate, ammonium stannate, and ammonium nitrate, smooth collagen metabolism and suppress skin aging. (Japanese Patent Laid-Open No. 5-246838)

On the other hand, octacosanol is a straight-chain aliphatic higher alcohol having the molecular formula CH ₃ (CH ₂ ) ₂₆ CH ₂ OH, which is separated from the extracted oil-retained fats and oils from rice bran, tangerine, wheat germ, etc. Effect.

Octacosanol is also an energy-releasing activator that has been shown to have a major influence on physical strength, muscle function, and basal metabolic rate, and has various functions to generate energy in muscle and body through catalysis. (The Korean Journal of Exercise Nutrition, Vol. 3, No. 2, 1999).

In addition, Korean Patent Application Laid-Open No. 2001-0066988 describes a composition in which octacosanol is contained in a reproductive type tea makes the skin beautiful and elastic. Due to the reproductive type of metabolism, the metabolism is active, the cell activity and regeneration is accelerated, the aging is delayed, the toxins of the human body are excreted, and the blood circulation is promoted. It contains octacosanol, which has the effect of increasing stamina, improving metabolic rate, and increasing energy burning rate, making the skin not only hard but also elastic.

Korean Patent Application Publication No. 1994-0703326 discloses that octacosanol is used to treat atherosclerosis complexes such as hypercholesterolemia and platelet hyperaggregation, ischemia and thrombosis, and to inhibit gastric ulcer induced drugs and improve male sexual function. It is.

When octacosanol is taken with fat, the cell count does not change and fat tissue is reduced. This seems to be because octacosanol interferes with the accumulation of lipids in tissue. However, it does not affect the amount of lipids in the liver (Kato S et. Al. Br J Nutr-1995. Mar-Octacosanol affects lipid metabolism on rats fed on a high fat diet).

Octacasanol inhibits platelet degradation and collagen degradation (Arruzabala MI et.al. Thromb reserch-1993 Fed-Effects of policosanol on platelet aggregation in rats).

The physiological activity of octacosanol improves exercise and endurance (Kabir Y. Kimura S, Ann Nutr Metab-1993-Biodistribution and metabolism of orally adminstered octcosanol in rats).

The octacosanol-containing waxes extracted from plant lipids such as onions, carrot peels, and wheat germ oil are processed as foods and used as dietary supplements. When used as dietary supplements, the quality of raw materials does not deteriorate. The color and gloss of silver must be alive and the octacosanol content must be at least 0.5%. In addition, the extraction solvent, etc. should not remain in the final food, and if it meets the standards and standards of food and food additives, it can be used as a health supplement food.

In the health food industry, octacosanol is also used for the purpose of alleviating systemic muscles and improving sensitivity, maintaining endurance for a long time, improving basic metabolic rate, and improving nutritional status for heart muscle. I use it.

An object of the present invention is to obtain a cosmetic composition having a skin moisturizing enhancement and anti-aging effect using octacosanol, which has a basic metabolic rate improving effect in food.

1 is a graph comparing the proliferative capacity of fibroblasts,

2 is a graph comparing the biosynthesis of collagen.

In order to achieve the above object, according to the present invention, there is provided a skin anti-aging cosmetic composition containing 0.01 to 5% by weight of octacosanol relative to the total weight of the cosmetic composition.

The present invention is described in detail as follows.

The octacosanol of the present invention can be prepared and used according to a known method, or a commercially available one can be purchased and used.

Such octacosanol can be added to all cosmetics related to anti-aging, such as softening cosmetics, nourishing cosmetics, nourishing cream, essence, massage cream, the addition amount is 0.01 to 5% by weight relative to the total weight of the cosmetic composition. If the addition amount is less than 0.01% by weight can not be expected anti-aging effect, when the amount is more than 5% by weight is not economical because the degree of increase in efficiency is not so high.

The following test examples, examples and comparative examples are intended to explain the present invention in more detail, and the scope of the present invention is not limited thereto.

(Test Example 1)

Fibroblast Proliferation Capacity Comparison Experiment

Octacosanol was supplied from Sigma (O3379, 1-Octacosanol) and a cosmetic raw material supplier, and used in Test Example 1.

Cell proliferation was tested using human skin derived fibroblasts. Fibroblasts were used by proliferating cells stored in -70 ° C or liquid nitrogen. Fibroblasts cultured in T-75 flasks were washed 2-3 times with PBS, and the adherent cells were dropped using trypsin, followed by centrifugation at 1500 rpm for 5 minutes (Heraeus, Labofuge 400R) to collect the cells. After centrifugation, the supernatant was discarded, and 10 ml of DMEM (10% FBS) was added to the precipitated cells and mixed evenly. By measuring the number of cells using a blood cell counter ⁴ to 10 ea / well degree of cell to each well of a 96 well it was dispensed by 200㎕. After culturing at 37 ° C and 5% CO ₂ , about 50% of the well area grows and exchanges medium with fresh DMEM (FBS10%), octacosanol by concentration (0.0001%, 0.001%, 0.01%, 0.1) %, 1%, 5%, 6%, 10%), cells were incubated for 6, 12, 24, 48 hours.

TT {(3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyl tetrazolium bromide (Sigma)} was dissolved in PBS to 2 mg / ml and stored in the refrigerator, Each well was treated with 20ul of MTT reagent (5mg / ml in PBS), incubated for 2 more hours, and then discarded the supernatant and mixed with 150µl of DMSO or 100µl of 0.04N-HCl. After the step, the absorbance was measured at 560 nm using an ELISA reader (μQuant, Bio-Tek instrument INC.) (Reference: 650 nm).

Cell proliferation was calculated according to the following equation, and the results are shown in FIG. 1.

In Figure 1, octacosanol showed a cell proliferation effect of about 70% compared to octacosanol untreated group after 48 hours at 0.01% treatment.

(Test Example 2)

Collagen Biosynthesis Effect

Fibroblasts incubated in T-75 flasks were washed 2-3 times with PBS, the adhered cells were dropped using trypsin, and the cells were collected by centrifugation (Heraeus; Labofuge 400R) at 1500 rpm for 5 minutes. After centrifugation, the supernatant was discarded, and 10 ml of DMEM (10% FBS) was added to the collected cells and mixed evenly. By measuring the number of cells using a hemocytometer, cells of about 10 ⁴ ea / well were dispensed into each 200-well well of 96 wells. After culturing at 37 ° C and 5% CO ₂ , about 50% of the well area grows and exchanges with fresh DMEM (FBS10%), octacosanol by concentration (0.0001%, 0.001%, 0.01%, 0.1%, 1%, 5%, 6%, 10%), cells were incubated for 6, 12, 24, 48 hours.

Collagen synthesis performance measurement kit (Procollagen Type-I C-peptide EIA kit (Takara, MK 101)) was used. For measurement using immunological methods, 100 μl of Ab-POD conjugate solution was transferred to one well and aliquoted, and 20 μl of cell culture or standard solution was added. The 96 well plate was wrapped with foil etc., and it incubated for 3 hours at 37 degreeC. After 3 hours, all the solutions in each well were removed and washed 4 times with PBS. After the washing step, 100 μl of substrate solution was added to each well and incubated at 20-30 ° C. for 15 minutes. In the final step, 100 µl of 1N H ₂ SO ₄ , the reaction termination solution, was added and mixed gently. The absorbance was measured with an ELISA reader and the result was used. After the reaction solution was added, the plate was stored at room temperature for about 1 hour and absorbance was measured at 450 nm.

Synthesis (%) was calculated according to the following equation, and the results are shown in FIG. 2.

In Figure 2, it can be seen that as shown in the results of cell proliferation, the enhancement effect is large.

(Examples 1 to 4 and Comparative Examples 1 to 4)

Essence was prepared by mixing the components of Table 1 (unit:%).

(Test Example 2)

Moisturizing and elasticity test

Moisture was measured by the high frequency impedance method using SKICON-200 (skin surface moisture content meter, IBS).

Twenty healthy men and women were banned from using moisturizers or lotions on their legs two weeks before the start of the trial. Examples 1-4 and Comparative Examples 1-4 were applied daily for 15 days. Test subjects were treated with the composition twice a day for three weeks, once in the morning and once in the evening. After 21 days, the test subjects were evaluated and averaged according to the following criteria.

0 = severely flaking, peeling

1 point = slightly dry skin

2 points = lightly dry skin

3 points = smooth, no evidence of dryness

In addition, in order to measure the elasticity, 20 women were divided into 5 groups of 100 women in their 30s to 40s. In each group, Examples 1 to 4 and Comparative Examples 1 to 4 were applied to the face once daily for 1 month, and then a cutometer (SEM575, Courage + Khazaka electronic GmbH) capable of measuring skin elasticity after 1 month was applied. Germany), the elasticity of the facial area was measured and compared with before application.

Skin elasticity was calculated according to the following formula.

The results are shown in Table 2 below.

(Test Example 3)

Wrinkle Relaxation Test

Twenty healthy women of 20-40 years old were treated with Examples 1 to 4 and Comparative Examples 1 to 4 twice a day, and then the tail of the eyes was measured. Replicas were made using silicone rubber (Silflo), and image analysis was performed using a Skin Visiometer (C + K) on the day of 0, 30 and 60 days. The evaluation method was based on three wrinkle measurements: wrinkle improvement (1), slight wrinkle improvement (2), and no wrinkle improvement (3).

The results are shown in Table 3.

In Table 3, Examples 1 to 4 showed an anti-wrinkle effect of more than 90% compared to Comparative Example 4 without the addition of octacosanol.

According to the present invention, by adding octacosanol, it is possible to obtain a cosmetic composition having an anti-aging effect through skin moisturizing, elasticity and wrinkle reduction.

Claims (1)

Cosmetic composition Anti-aging cosmetic composition containing 0.01 to 5% by weight of octacosanol relative to the total weight.