KR20000007943A - Whitening cosmetic containing extracts from a bamboo endothelium - Google Patents

Abstract

PURPOSE: A whitening cosmetic containing an extract from the endothelium of Phyllostachys pubescens MAZEL is provided, which shows excellent whitening effect without skin irritation and gets over the problem like as an eye-sight change during manufacturing and keeping of product. CONSTITUTION: The whitening cosmetic contains 0.0001¯5%, desirably 0.001¯3% of the dried bamboo endothelium on the basis of total cosmetic weight. The bamboo endothelium is gained as a by-product from a process of producing the 2nd layer skin using in bamboo works. The next extraction process comprises of: crushing to pieces after drying; extracting with a solution mixed as 8:2 of ethanol or methanol and waters, or ethanol or methanol individually; keeping this for 1¯15 days at 15¯37 C; concentrating this extract under the decompression using a distiller eqiupped with a cooling condenser; and drying.

Description

Whitening cosmetics containing bamboo extract

The present invention relates to a whitening cosmetics, and more particularly to a whitening cosmetics containing bamboo endothelial extract extracted from the inner endothelium of bamboo (Phyllostachys pubescens MAZEL) as a whitening active ingredient.

Human skin color depends largely on the amount of melanin pigment contained in skin cells. People with high levels of melanin have brown or black skin, while people with very low levels of melanin cause vitiligo. Melanin pigment is produced from tyrosine by tyrosinase in pigment cells called melanocytes, which are present in the basal membrane of the skin.

Melanin pigments play an important role in protecting the skin from excessive ultraviolet rays, thereby preventing skin damage and the occurrence of skin cancer. However, excessive production of melanin due to sunlight, hormonal changes, inflammation, drugs, etc. deposits skin pigments, which causes a variety of cosmetic problems. For example, skin pigmentation, freckles, and freckles are caused by an increase in the amount of melanin pigment, which means that the production of melanin pigment is promoted or the number of melanocytes is increased.

At present, in the development of a whitening agent for skin beauty, a method of reducing the generated melanin pigment by decolorization and a method of inhibiting the activity of tyrosinase, an enzyme that forms melanin pigment, are known. However, whitening agents using tocopherols or vitamins, which are used to reduce the melanin pigment, are known to have a slight decolorizing effect of the melanin pigment. Therefore, attention is being paid to inhibitors that inhibit the production of melanin by inhibiting the activity of tyrosinase.

Tyrosinase is an enzyme that catalyzes the reaction of oxidizing monophenols into melanocytes of higher animals. By this enzyme, tyrosine is oxidized to dopaquinone, and melanin pigment is produced through a series of chemical reactions and oxidation. This melanin pigment is brown or black, which absorbs excessive light in vivo and thus adversely affects skin beauty.

In the selection of whitening agents, currently widely used methods are to select inhibitors that inhibit tyrosinase activity using tyrosinase purified from mushrooms and to evaluate the status of melanoma cells against whitening sources using mouse melanoma cells. There is a way to select. The former has the advantage of being simple and saves time and cost, but has the disadvantage that only tyrosinase inhibitors can be selected, and the latter can more comprehensively select whitening agents but has the disadvantage of costly, time and effort. .

In view of the fact that inhibiting the activity of tyrosinase enzymes can inhibit the production of melanin pigments, in the conventional cosmetics field, hydroquinone, tretinoin, kojic acid, arbutin, ascorbic acid, glutathione, etc. as a whitening ingredient are used as inhibitors of tyrosinase activity. The method of use has been used. However, when the cosmetics are manufactured using the above components, there are disadvantages such as discoloration due to oxidation by reaction with light during the manufacturing process or product storage. Therefore, additives such as antioxidants, UV absorbers, and metal chelating agents were added to reduce such discoloration. However, when a large amount of such additives are used, there is a problem in skin stability, such as irritating the skin, and there is a disadvantage in that sufficient whitening effects cannot be obtained as desired.

Therefore, while using a small amount of additives compared to the conventional whitening agent has an excellent whitening effect, there have been many studies to develop a whitening agent without side effects such as irritation to the skin.

The present inventors have searched for substances effective in preventing hyperpigmentation among natural products to solve the problems of conventional whitening materials and to find raw materials exhibiting better whitening effects. The present invention has been accomplished by finding that it inhibits the formation of.

Bamboo is also known as bamboo sundae (dough) and is an evergreen naturalized plant native to China and native to the south. Bamboo is used for edible, ornamental, and industrial purposes. In Chinese medicine and in private, bamboo stalks, leaves, or bamboo shoots are vomited, anti-inflammatory, spans, poisons, lactic acid, It is used for medicines such as ripening, hemolithiasis, gold spear, remedy, tetanus, sweating, pain, and stroke. .

Bamboo skin used in the present invention refers to a white film on the inner surface of the bamboo, it is used to peel the film after splitting the bamboo. Juknapi has the same effect as the green skin of bamboo, the first layer of skin and the white layer of white layer on its inner layer. The present invention is applied to the above-mentioned bamboo endothelium as a raw material of the whitening cosmetics, such as supple cosmetic water (skin), nutrient cosmetics (lotion), nutrition cream, massage cream, essence or pack.

It is an object of the present invention to provide a whitening cosmetic which has excellent whitening effect and no side effect such as skin irritation compared to the conventional whitening agent, despite the use of the same amount of whitening raw material.

1 is a graph showing the activity inhibition rate of tyrosinase

2 is a graph showing the amount of melanin pigment

The whitening cosmetics according to the present invention contains 0.0001 to 5% of dried bamboo flakes, preferably 0.001 to 3%, based on the total weight of the cosmetic.

Hereinafter, the present invention will be described in detail.

1 is a graph showing the degree of inhibiting the activity of tyrosinase, a graph showing the amount of sample required to inhibit the activity of tyrosinase by standard deviation.

2 is a graph showing the total amount of melanin added to the original melanin contained in the experimental cells and the increased melanin amount after culturing with each sample, the standard deviation of the total amount of melanin when each sample is used It is represented as.

The inner endothelium of bamboo is obtained as a by-product along with the first layer of blood used as a medicinal herb in the process of producing the second layer of blood used mainly for bamboo work. The obtained bamboo endothelium is dried and finely pulverized, and then extracted with ethanol or methanol and a solution of 8: 2 as a solvent, or ethanol or methanol alone. The bamboo bark extract is obtained by immersing the dry bamboo bark in the extraction solvent at 15-37 ° C. for 1-15 days. The bamboo shoots extracted in this way were concentrated under reduced pressure completely while recovering the solvent evaporated using a distillation apparatus equipped with a cooling capacitor, and then dried. The dried bamboo shoots were 0.0001-5%, and preferably 0.001-3% whitening cosmetic. Add to

The present invention will be described with reference to the following Examples, Experimental Examples, and Prescription Examples, but is not limited thereto, and various changes of Examples, Experimental Examples, and Prescription Examples are possible within the spirit and scope of the present invention.

(Example)

Example 1

100 g of the inner endothelium of bamboo was dried and finely pulverized, and then put in 3 L of 80% methanol and extracted at 15-37 ° C. for 14 days. The extract was filtered through a 300 mesh filter cloth, left to mature for 5 days at 5 to 15 ° C, and then filtered through a filter paper. The extract was concentrated under reduced pressure at 70 ° C. in a distillation apparatus equipped with a cooling capacitor to obtain 1.07 g of dried dead skin extract.

Example 2

100 g of bamboo endothelium was dried and finely pulverized, and then placed in 3 l of 80% ethanol and extracted at 15-37 ° C. for 14 days. The extract was filtered through a 300 mesh filter cloth, left to mature for 5 days at 5 to 15 ° C, and then filtered through a filter paper. The extract was concentrated under reduced pressure at 70 ° C. in a distillation apparatus equipped with a cooling capacitor to obtain 1.93 g of dried bamboo extract.

Experimental Example

Experimental Example 1

Tyrosinase inhibitory effect was measured on the dried bamboo endothelium extract obtained in Example 1 and arbutin. Tyrosinase is an enzyme purified from potatoes and mushrooms. In this experiment, Sigma company extracted from mushrooms was used.

First, the substrate tyrosine was dissolved in sodium phosphate buffer (0.1M, pH6.8) to make a solution having a concentration of 0.3 mg / ml, and the solution was taken in a test tube with 334 µl, and then again buffered with sodium phosphate 576 μl of solution (0.1M, pH6.8) was added. The dried bamboo bleed extract of Example 1 was dissolved in methanol to 10 mg / ml, and then diluted to 5, 1.5, 1 mg / ml, 500, 150, and 100 µg / ml using methanol to make an experimental group. 70 μl of each of these diluted extract samples was added to the in vitro solution and allowed to react for 10 minutes in a 37 ° C. thermostat.

As a control, 70 μl of methanol was added instead of the endothelial extract. 20 µl of 1700 unit / ml tyrosinase solution was added to the reaction solution of the experimental group and the control group so that the total volume was 1 ml, and the reaction was continued for 30 minutes in a 37 ° C thermostat. The test tube containing the reaction solution was placed in iced water and quenched to stop tyrosinase activity, and then absorbance at 470 nm was measured with a photospectrometer.

The blank of the experimental group was the reactant except the tyrosinase solution, that is, the tyrosine, the bamboo endothelium extract and the sodium phosphate buffer, and the blank of the control group was added methanol instead of the bamboo endothelium extract in the blank of the experimental group.

When measuring the inhibitory effect of tyrosinase activity on arbutin, arbutin was dissolved in sodium phosphate solution to a concentration of 10 mg / ml, and then again using sodium phosphate buffer solution, 5, 1.5, 1 mg / ml, 500, It diluted to 150 and 100 microgram / ml, and used it as an experimental group. The blank of the experimental group of arbutin is the reactant excluding tyrosinase, ie tyrosine, arbutin solution, sodium phosphate buffer solution. The control group of arbutin was a sodium phosphate buffer solution instead of the arbutin solution, and the blank of the arbutin control group was a reaction product except tyrosinase solution, that is, tyrosine and sodium phosphate buffer solution. Table 1 summarizes the above contents.

Inhibition rate of tyrosinase against bamboo endothelium extract and arbutin was obtained by the following equation. The experimental results are shown in Table 2.

A: absorbance of the control group B: blank absorbance of the control group

C: absorbance of the experimental group D: blank absorbance of the experimental group

Material used Experimental group Blank of Experimental Group Control Blank of Control Tyrosine (0.3 mg / ml) 334 μl 334 μl 334 μl 334 μl Sodium Phosphate Buffer Solution (0.1M, pH6.8) 576 μl 596 μl 576 μl 596 μl sample 70 μl 70 μl 70 μl 70 μl Tyrosinase solution (1700 units / ml) 20 μl - 20 μl - Note) Total volume 1 ml

sample Sample concentration that inhibits tyrosinase activity by 50% (IC 50: μg / ml) Bamboo Blood Extract 242.30 ± 93.15 Arbutin 722.23 ± 348.77 Note) The above figures are expressed as mean ± standard deviation.

As shown in Table 2, it can be seen that the porridge extract has about 3 times higher ability to inhibit tyrosinase activity than arbutin.

Experimental Example 2

The inhibitory effect of the production of melanin pigment on the bamboo endothelium extract was measured.

Pigmented cells derived from mouse melanoma cells were grown in culture by standard methods. In this experiment, cell lines of B-16 cells (ATCC, USA) were used, but other cell lines or human melanocytes can be used.

Melanoma cells were supplied to RPMI (Gibco) cell culture medium containing 10% fetal bovine serum, 1% antibiotic, and 10 ^-7 M α-MSH (Meline Cell Stimulating Hormone, manufactured by Sigma). It grew to about 3 confluences. Subsequently, each sample, for example, the dried bamboo extract, kojic acid, arbutin and ascorbic acid obtained in Example 1, was dissolved in the culture medium at a concentration of 50 µg / ml, and the solution containing the sample was then black. After addition to the species cells were incubated for 2 days or more. The control group was put in a culture medium containing no sample. After incubation, the cells were treated with trypsin-EDTA (Gibco Co., Ltd.), collected in a test tube, and counted. The cells were dissolved in melanin by adding 1 ml of 1N hydroxide solution per 4,000,000 cells, and the absorbance at 470 nm was measured and analyzed. . The experimental results are shown in Table 3.

sample Melanin amount (µg / ml / 4,000,000 cells) % Inhibition Control 97.17 ± 18.85 - Bamboo Blood Extract 43.79 ± 15.50 54.9% Arbutin 51.79 ± 8.90 46.7% Kojisan 49.83 ± 8.25 48.7% Ascorbic acid 137.03 ± 41.39 -41.0% NOTE The above figures are expressed as mean ± standard deviation.

As can be seen in Table 3, the bamboo endothelium extract can inhibit the amount of melanin in B-16 melanoma cells compared to the control group, and the amount of melanin produced is lower than that of the same amount of arbutin or kojic acid. Can be.

(Prescription example)

Prescription Example 1: Nutritional Cream

As follows, the nourishing cream containing the bamboo endosperm extract obtained in Example 2 was prepared according to a conventional method.

ingredient Content (% by weight) Bamboo Blood Extract 0.1 Liquid paraffin 6.0 Squalane 4.0 Sorbitan sesquioleate 0.5 Polysorbate 60 1.5 beeswax 10.0 Capric & Capric Triglycerides 5.0 Concentrated glycerin 6.0 Propylene glycol 3.0 Butylene glycol 2.0 Triethanolamine 0.2 antiseptic a very small amount Pigment a very small amount Spices a very small amount Purified water Balance

Prescription Example 2: Nutritional Cosmetics

As follows, the nutrient lotion (lotion) containing the bamboo endosperm extract obtained in Example 2 was prepared according to a conventional method.

ingredient Content (% by weight) Bamboo Blood Extract 0.1 Cetanol 1.0 Stearyl alcohol 1.0 Glycerin Monostearate 3.0 Sorbitan monostearate 1.0 Polyoxyethylene sorbitan monooleate 2.0 vaseline 2.0 Liquid paraffin 6.0 Squalane 5.0 Octyldodecyl Myristate 5.0 glycerin 5.0 1,3-butylene glycol 5.0 Carboxy Vinyl Polymer 0.3 Triethanolamine 0.3 antiseptic a very small amount Pigment a very small amount Spices a very small amount Purified water Balance

Formulation Example 3: Softening Cosmetics

As described below, the softening water (skin) containing the bamboo endosperm extract obtained in Example 2 was prepared according to a conventional method.

ingredient Content (% by weight) Bamboo Blood Extract 0.1 Concentrated glycerin 2.0 Propylene glycol 3.0 Butylene glycol 2.0 ethanol 10.0 Carboxy Vinyl Polymer 0.1 Triethanolamine 0.1 antiseptic a very small amount Pigment a very small amount Spices a very small amount Purified water Balance

Prescription 4: Essence

As follows, the essence containing the bamboo endothelium extract obtained in Example 2 was prepared according to a conventional method.

ingredient Content (% by weight) Bamboo Blood Extract 0.1 Pluylene glycol 5.0 glycerin 10.0 1,3-butylene glycol 10.0 Polyethylene Glycol 1500 5.0 Carboxy Vinyl Polymer 0.5 Hydroxyethyl cellulose 0.5 Triethanolamine 0.5 ethanol 5.0 Polyoxyethylene nonylphenyl ether 0.7 antiseptic a very small amount Pigment a very small amount Spices a very small amount Purified water Balance

Prescription 5: Massage cream

As follows, the massage cream containing the bamboo endosperm extract obtained in Example 2 was prepared according to a conventional method.

ingredient Content (% by weight) Bamboo Blood Extract 0.1 Liquid paraffin 40.0 Squalane 5.0 Sorbitan sesquioleate 0.8 Polysorbate 60 1.5 beeswax 10.0 Capric & Capric Triglycerides 4.0 Concentrated glycerin 6.0 Propylene glycol 2.0 Butylene glycol 4.0 Triethanolamine 0.2 antiseptic a very small amount Pigment a very small amount Spices a very small amount Purified water Balance

Prescription 6: Pack

As follows, a pack containing the bamboo endosperm extract obtained in Example 2 was prepared according to a conventional method.

ingredient Content (% by weight) Bamboo Blood Extract 0.1 Allantoin 0.2 ethanol 5.0 Nonylphenyl Ether 0.3 Sodium Carboxymethyl Cellulose 0.2 Polyvinyl alcohol 13.0 antiseptic a very small amount Pigment a very small amount Spices a very small amount Purified water Balance

From the above test results, the bamboo endothelium extract of the present invention is superior to the conventional arbutin or koji acid melanin pigmentation inhibitory effect, it can be seen that the skin whitening effect is excellent.

Claims (5)

Whitening cosmetics containing bamboo extract. The whitening cosmetic of Claim 1 which contains 0.0001 to 5% of bamboo endosperm extract on dry weight basis with respect to the whole cosmetics. The whitening cosmetic of claim 1, wherein the extraction solvent is ethanol or methanol. The whitening cosmetic according to claim 1, wherein the extraction solvent is a mixed solvent of ethanol or methanol and water. The whitening cosmetic according to claim 3 or 4, wherein the bamboo bark extract is obtained by extracting bamboo bark by immersing the bark bark in the solvent at 15 to 37 ° C for 1 to 15 days.