KR19990079378A - Cosmetics containing natural products that are effective in treating and preventing acne - Google Patents

Abstract

The present invention relates to a natural product-containing cosmetics effective for acne. In the field of acne treatment, various medicines have been developed and used, but the healing effects are insignificant, frequent relapses, and no preventive concept exists. In addition, the development of cosmetics for acne skin care has not been studied yet, and there is no product of a healing concept.

Therefore, the present invention is to develop a cosmetic that can prevent as well as prevent acne, and to provide a cosmetic that is effective in the prevention and healing of acne by applying white paper, yellow lotus, gold, licorice or burdock among natural products.

Description

Cosmetics containing natural products that are effective in treating and preventing acne

The present invention relates to a natural product-containing cosmetics effective for acne. More specifically, the present invention relates to cosmetics that are effective in the treatment and prevention of acne by containing at least one natural product selected from white paper, rhubarb, licorice, golden and burdock.

Acne refers to chronic, refractory and inflammatory skin diseases that occur in hair follicles and sebaceous glands. The causes of acne are largely increased sebum secretion, stenosis caused by hyperkeratosis of hair follicles, and growth of acne bacteria. , Inflammatory reactions, etc. can be explained according to the development of acne symptoms, and various mechanisms may work in combination.

That is, estrogen, a sex hormone that is increased after puberty, may excessively increase sebum secretion and cause narrowing of hair follicles. In addition, the lipid composition of sebum is different from the epidermis of the skin, so it is well oxidized and is used as an energy source of acne bacteria, so the increase of sebum is considered to be the first factor that can cause acne.

Acne bacteria are also anaerobic bacteria that do not multiply in the open hair follicles. In other words, when sebum increases and hyperkeratosis of hair follicles progresses, the entrance of the hair follicles is blocked, resulting in acne, an early lesion of acne, and excessive proliferation of acne bacteria.

Increased acne bacteria metabolize the lipids of sebum to produce various fatty acids that are stimulants, and acne bacteria are recognized as antigens and stimulate the body's immune response to cause inflammatory reactions. This causes papules and inflammatory acne lesions, and when deteriorated, the follicle wall is destroyed and tissues are damaged, such as cysts and pustules.

Although various medicines have been developed and used in the treatment area of acne, the selection of cosmetics for proper skin care has not been studied yet.

In the cosmetics area, mainly soap, medicated soap, abrasive cleanser, etc., is a concept that heals acne by removing hair follicle narrowing through skin peeling. In addition, the alcohol component of astrigent also exfoliates the skin and disinfects the acne. There are also cosmetics containing resorcinol, sulfur, salicylic acid and benzoyl peroxide. However, these raw materials have a lot of side effects and limitations such as skin irritation due to the restriction of the use concentration.

In addition, antibiotics such as erythromycine for the removal of acne bacteria, anti-inflamation ingredients for inflammation treatment, and hormone preparations for sebum control are used, but these are mainly ingredients in the pharmaceutical domain.

To date, products have been developed with a focus on only one of the many mechanisms of acne described above, so the healing effects are negligible, frequent relapses, and no preventive concept. In addition, there is no healing product in the cosmetic field.

Therefore, the present inventors have conducted extensive research to develop cosmetics that can prevent as well as heal acne, and found that white paper, yellow lotus, gold, licorice and burdock among natural products are effective in the treatment and prevention of acne. The invention was completed.

That is, an object of the present invention to provide a cosmetic that is effective in the prevention and healing of acne containing white paper, yellow lotus, golden, licorice or burdock.

Cosmetics effective for acne according to the present invention for achieving the above object is characterized in that it contains at least one selected from the group consisting of white paper, yellow lotus, golden, burdock and licorice among natural products.

Cosmetics according to the present invention is prescribed so as to cause skin irritation in the oil component or to remove all lipids similar to the lipid component of sebum so as not to induce scrim and inflammation. In addition, it is possible to cope with all the action mechanisms that cause acne by mixing natural substances such as anti-sebum, soybean, burdock, golden extract, licorice which has excellent antibacterial activity against acne bacteria, and white paper, which is an anti-inflammatory substance, to suppress excessive sebum. It consists of raw material composition.

Natural products used in the acne cosmetics according to the present invention are as follows.

1) white paper

Scientific name: Angelica daurica

Plants: perennial grass 1 to 1.5m high. In summer, small white flowers gather at the ends of branches. Fruits are small oval, and white paper is the name for the root. Roots are used to dig up the roots of young plants that do not have a flower stalk in the fall, peel off the outer skin, and dry them in the sun.

Ingredients: Root (white paper) contains about 0.3-0.9% of coumarins such as imperatorin, peloputerin and xanthoroxine, and contains more than 0.2% essential oils. It also contains components such as pelardene, hydrocarotene, and oxypentadepic acid.

Action: White paper has pain relief sedation. This is known to be due to the furokmarin component. In addition, copper decoction solution was found to have anti-cancer activity in animal experiments.

Application: Used in sedatives, sore throats, blood drops, colds, and head aches in motion therapy. It is also used as a pain medication after giving birth and is also used for rheumatoid and spinal cord myositis.

2) burdock

Scientific name: Arctium Lappa

Plants: perennial plant 1 to 2 m high. Wide or long egg-shaped leaves are alternately attached and violet red flowers gather at the end of branches and bloom in summer like a round ear. The fruit is picked up in the autumn, dried in the sun, and then crushed. Roots are used for digging in the autumn, washed in water and dried.

Ingredients: Berries contain lignan glycosides, arcatyne, oil 25-30%, phytosterin, vitamin B1, saponin, coumarin, lemon acid, malic acid, inulin 27-45%, protein 12%, essential oil 0.17%, It contains Arcane, Caffeic Acid, Tannin, and Alkaloids.

Action: Fruit has a diuretic, anti-inflammatory, drainage effect. In addition, the water extract of the fruit has an antimicrobial action against filamentous fungi causing skin disease. Roots have a diuretic and metabolic action. Alkaloids also have anticancer activity.

Application: In motion therapy, the fruit is used for inflammatory drugs, fever lowering medicines, colds, wounds, skin diseases, malignant tumors, etc. In addition, the root is used for gastritis, gastroduodenal ulcer, etc.

3) yellow lotus

Scientific name: Coptidis Rhizoma

Plant: Root stem with almost no root of Coptis japonica contains at least 3.5% berberine when quantified. Uneven conical, 2-4cm, the outer side is gray brown, and the bottom of stem and many roots can be seen.

Ingredients: Berberine is the main ingredient, in addition, it contains palm martin. It also contains trace amounts of alkaloids of about 3-7%.

Action: Water extract has lipase action and pepsin lowering effect. Methanol extract has anti-inflammatory effect and prevents atherosclerosis. In addition, 50% ethanol extract is antimicrobial activity against staphylococcus aureus, erythrocytes, cholera bacteria and 35% ethanol extract has a jingyeong effect on the above small intestine.

Application: It is used as a raw material for gastrointestinal and branch medicine in Chinese medicine.

4) Licorice

Scientific name: Glycyrrhiza glabra

Plants: attached to the main goblets, attached to the plant, shoots, leaves, has an unusual smell, tastes sweet. Use both peeled and peeled off.

Ingredients: Triterpene glycosides, glabric acid, flavonones, isoflavones and other glycosides.

Action: Glycyrrhizin or genin, the main component, activates estrogen and has anti-inflammatory, detoxification and anti-allergic effects. In addition, fractions containing a large amount of flavonoids have anti-ulcer action and mycelial action.

Application: In oriental medicine, it is widely used for cramps, muscle pain, etc. for the purpose of pain relief and detoxification.

5) golden

Scientific name: Scutellaria baicalensis

Plant: It means the root of peeled grass of the decayed grass. The decayed grass is yellowish brown with vertical wrinkles, and the quality is hard, soft and weak smell, and the taste is bitter.

Ingredients: Flavonoids, baicalin, chrysin and other free amino acids.

Action: It is prescribed for diseases involving anti-inflammatory, fever and abdominal pain.

In order to determine the efficacy of the natural acne as described above was tested.

Test Example 1: Inhibitory Effect on Chemotaxis of Acne Bacteria by Natural Product Samples

1. Experiment Method

end. Neutrophils leukocyte isolation:

The leukocyte layer and erythrocyte layer were separated by Ficoll-paque methods followed by polymorphonuclear leukocytes with 3% Dextran solution.

I. Chemotaxis test and its inhibitory effect

1) Experimental Principle: Neutrophil leukocytes are moved by P. acnes to induce inflammatory effects. The movement of leukocytes is by chemotaxis. When natural products, erythromycin and tretinoin are applied to acne bacteria, they inhibit chemotaxis and have anti-inflammatory effects.

2) Experimental method: The chemotaxis induced by 1% P. acnes solution was tested using natural products and erythromycin, a positive control.

3) Evaluation method: 48-well micro chemotaxis method was used to observe the neutrophil leukocytes migrated by acne bacteria chemotaxis in a 400-fold optical microscope to determine whether chemotaxis was induced or inhibited. Comparison was made.

2. Experimental Results

The results of testing the chemotaxis test and its inhibitory effect induced with 1% P. acnes solution are shown in Table 1.

Chemotaxis test induced by 1% P. acnes solution and its inhibitory effect matter 1% P.acnes Erythromycin 0.01% 0.01% of white paper Licorice 0.01% Sulfur 0.01% Burdock 0.01% Percentage of migrated neutrophil leukocyte count 100.00 29.76 33.70 66.67 61.95 62.35

As shown in Table 1, the application of white paper, licorice, rhubarb, burdock is relatively small neutrophils leukocytes transferred, it can be seen that there is an effect of suppressing white paper, licorice, yellow lotus, burdock acne bacteria.

[Test Example 2: Confirmation of antimicrobial activity against acne bacteria (P. acnes)]

Antibacterial activity test against P. acnes was done by liquid medium dilution method.

Each sample was diluted in 0 ppm to 1000 ppm in 100 ppm intervals in 2 ml of liquid BHI medium, and then inoculated with 20 μl of 10 ⁶ / ml of the bacterial solution and incubated for 48 hours. The minimum concentration at which growth was inhibited based on turbidity was defined as the minimum growth inhibition concentration (MIC).

P. acnes ATCC 6919 and ATCC 11827 were used as strains. The results are shown in Table 2.

Antimicrobial test results against P. acnes sample Minimum growth inhibition (ppm) P. acnes ATCC 6919 P. acnes ATCC 11827 blank 1700 1850 Gold 1000 500 goldthread 1050 1100 licorice 400 400 burdock 1400 1400

As shown in Table 2, it can be seen that the antibacterial activity against golden acne bacteria, golden, yellow lotus, licorice is particularly excellent.

Test Example 3. Lipogenesis Synthesis Test

The sebaceous glands are many hamster biopsy (biopsy) the ear tissue in order to assess the degree of lipid synthesis inhibition on sebaceous gland by the method for evaluating the consumption of the carbon element (uptake) Lipid synthesis inhibitors to quantify the extent to the extent necessary for lipid synthesis is C ¹⁴ Incubated for 6 hours in a labeled medium and the degree of inhibition was assessed by comparative quantitation of the control and the sample group using a radiometer. As a sample group, 0.01% of the sample solution was added to the medium using hamster right ear tissue, and 0.01% of physiological saline was added to the medium using the hamster left ear tissue as a control group. The results are shown in Table 3. In Table 3, the inhibition rate was calculated by Equation 1 below, and the value is an average value performed for four hamsters.

Lipogenesis Inhibition Test Results sample % Inhibition goldthread 72 burdock 52 licorice 18 blank 22

As shown in Table 3, it can be seen that rhubarb, white paper, burdock, licorice have an inhibitory effect on lipid synthesis. In other words, by inhibiting lipid synthesis, sebum can be reduced to suppress the occurrence of acne.

[Test Example 4: Human skin primary stimulation test]

It can be seen from the results of Test Example 1-3 that white paper, yellow lotus, golden, burdock, and licorice have an acne treatment and preventive effect, and to determine whether the natural products are harmful to human body when applied to cosmetics. Human skin irritation test was done.

Human skin primary stimulation test was a human patch test (human patch test). That is, 30 healthy women aged 20-30 years old were tested according to CTFA guidelines (The Cosmetic, Toiletry and Fragrance Association. Inc. Washington, D.C., 20036, 1991). 20 μl of the test substance (10% in patch base) prepared in the Finn Chamber was added dropwise, washed with 70% ethanol, dried, and fixed on the back of the test site with a micropore tape. . The patch is applied for 24 hours, and after removing the patch, the marking area is marked with a marking pen. After 30 minutes and 24 hours, the skin reaction of each test site is observed and the International Contact Dermatitis Research Group ; ICDRG). The results are shown in Table 4.

In Table 4, the average reactivity was calculated according to Equation 2 below, ± was 1 point, + was 2 points, ++ was 3 points, and +++ was 4 points. The criterion was based on less than 1 grade I (non-irritating range), less than 3 grade II (light stimulation range), less than 5 grade III (medium stimulation range), and 5 or more grade IV (strong stimulus range). .

Test substance 24 hours 48 hours Average reactivity (n = 30) Judgment grade ± + ++ +++ ± + ++ +++ Acne base 5 - - - One - - - 2.50 Ⅱ Base + white paper0.4% 6 - - - One - - - 2.92 Ⅱ Base + Burdock 0.4% 6 - - - - - - - 2.75 Ⅱ Base + Licorice 0.4% 6 - - - - - - - 2.75 Ⅱ Base + Golden 0.4% 7 - - - - - - - 2.98 Ⅱ Base + Sulfur 0.4% 6 - - - - - - - 2.75 Ⅱ Base + white paper 0.4% + burdock 0.4% 7 - - - - - - - 2.98 Ⅱ Base + Golden 0.2% + Sulfur 0.03% + Licorice 0.2% 6 - - - - - - - 2.75 Ⅱ

As shown in Table 4, the Acne base exhibited a safe range of stimulus values but tended to be somewhat higher. All raw materials showed grade II or higher stimulation at the test prescription. However, even though the experiment was carried out at low concentrations because of pigmentation problems, it showed the same stimulus and relatively high stimulus. In the case of a combination of various ingredients, the level of safety was Grade II (less than 3 stimulus level).

[Example 1-25]

Following the manufacture of cosmetics containing natural products in the compositions of Tables 5 and 6, the sebum amount change, the efficacy evaluation and the side effects evaluation of the prepared cosmetics, respectively, the results are shown in Table 7.

Evaluation of sebum volume change: The sebum volume change was evaluated through the reduced ratio by comparing the results before and one month after treatment with a semeter.

Efficacy evaluation: The number of acne in a certain area and a certain area of the face before the treatment was counted, and the number of acne was counted again one month after the treatment. For example, 50% of effectiveness means that 50% of all the subjects have reduced acne by more than 20%.

Side effects evaluation: The presence or absence of side effects such as irritation through the survey of the subjects, and the dermatologist, the evaluator, directly evaluated the skin condition of the subjects to determine the side effects.

Furtherance content(%) Purified water to 100 glycerin 4.5 Ethylenediaminetetradiacetic acid sodium Quantity Allantoin 0.1 Triethanolamine 0.12 Cyclomethicone 5.0 Dimethicone / Vinyl Dimethicone Crosspolymer 3.0 Dimethicone 3.0 Polyglyceryl-3methylglucose distearate 2.5 incense Quantity antiseptic Quantity Carboxy Vinyl Polymer 0.12 Natural products (one or more of white paper, rhubarb, golden, burdock and licorice) Type and content see Table 6

In the following Table 6, "○" designation indicates that the natural product marked "○" is additionally used in the composition of Table 5. In addition, the amount of natural products used in each example is 0.2% white paper, 0.03% rhubarb, 0.2% licorice, 0.2% gold, 0.2% burdock.

Natural products Example One 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 blank ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ goldthread ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ licorice ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ Gold ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ burdock ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○

Example Sebum change Effectiveness evaluation Side effects assessment One No change 45% Weak erythema, itching 2 No change 45% Weak erythema, itching 3 No change 43% Weak erythema, itching 4 No change 45% Weak erythema, itching 5 20% reduction 50% Weak erythema, itching 6 20% reduction 46% Weak itching 7 20% reduction 50% Weak erythema, itching 8 15% reduction 46% Weak erythema, itching 9 20% reduction 48% Weak erythema, itching 10 No change 50% Weak erythema, itching 11 No change 50% Weak erythema, itching 12 No change 27% Weak erythema, itching 13 40% reduction 0 Weak erythema, itching 14 20% reduction 13% Weak erythema, itching 15 13% reduction 21% Weak erythema, itching 16 No change 48% Weak erythema, itching 17 No change 29% Weak erythema, itching 18 10% reduction 21% Weak erythema, itching 19 10% reduction 15% Weak erythema, itching 20 No change 23% Weak erythema, itching 21 No change 19% Weak erythema, itching 22 No change 20% Weak erythema, itching 23 No change 21% Weak erythema, itching 24 15% reduction 48% Weak erythema, itching 25 10% reduction 18% Weak erythema, itching

As shown in Table 7 above, the case of Example 11 was the most ideal, and the example containing no white paper had an effectiveness of about 20%, while the example containing white paper showed 40-50% of effectiveness. You can see that the ingredients are good for acne and synergistic with other natural products. In the case of side effects, mild erythema and itching were observed in the first week after use in all examples, but the degree was not enough to determine the stimulus.

As described above it can be seen that the cosmetic according to the present invention is effective in the treatment and prevention of acne by containing white paper, golden, yellow lotus, licorice or burdock.

Claims (1)

Cosmetics effective for acne, characterized in that it contains at least one natural product selected from the group consisting of rhubarb, burdock, golden, licorice and white paper among the natural products.