KR19980048554A - Treatment of mastitis in cows, cholesterol lowering and somatic cell lowering in milk - Google Patents

Abstract

The present invention is a low molecular weight (molecular weight less than 10,000) chitin, chitosan, which is isolated and purified from chitin, known as a functional new material of food, to chitin and chitosan, and decomposed products of chitin and chitosan by chemical or enzymatic methods from chitin and chitosan. Or chitooligosaccharides for mixed feed preparations or powders, liquid feed additives, or animal drugs that can be expected to treat cow mastitis, reduce somatic cell counts, and lower milk cholesterol, It is about.

Description

[Name of invention]

Treatment of mastitis in cows, cholesterol lowering and somatic cell lowering in milk

[Brief Description of Drawings]

1 is a chemical structure of chitin, according to the present invention,

2 is a process for separating and purifying chitin in accordance with the present invention,

3 is a process chart for preparing low molecular weight chitin, chitosan and chitooligosaccharide by enzyme treatment according to the present invention.

4 is a process chart for preparing low molecular weight chitin, chitosan and chitooligosaccharides by chemical treatment according to the present invention.

Detailed description of the invention

Technical Field

The present invention relates to a drug that can suppress the increase of somatic cell count in milk by developing feed for cows in the field of livestock, lowering cholesterol level in milk, enhancing the immunity of animals, enhancing disease resistance and maintaining health. .

Background of the Invention

Animal protein and fat intake is over 50% of total protein and fat intake per person due to the quality and quantity of people's diet. Recent Ministry of Health and Welfare statistics show that the main cause of death in Korea is related to cerebrovascular disease, heart disease, hypertension, diabetes, and liver disease. The problem of western diet is the excessive increase in blood cholesterol level, which can be a direct cause of various adult diseases. Today, even adolescents have become known as adult diseases.

Cholesterol is not an essential nutrient because it is used as a precursor for synthesizing vitamins and various hormones, or because it is synthesized in the human body as a component of cell membranes. As a fat-soluble substance, it is widely distributed in animal foods, and according to the diet, a large amount of cholesterol can be ingested. In this case, the liver regulates cholesterol synthesis to maintain blood levels.

However, if excess cholesterol can be continuously consumed from food, it exceeds the limit of metabolic control capacity and raises blood cholesterol level and deposition on blood vessel wall occurs.

Conventionally, many studies have been conducted to reduce the cholesterol content in various meats, eggs, and milk foods as a rather consumable food. However, until now, it has not been effective and various antibiotics are indiscriminately used for the prevention or treatment of diseases. As a result, various problems are raised due to antibiotic residues and the appearance of resistant bacteria.

In particular, cholesterol content in milk products contains about 12mg per 100g, so it has been recognized that its content is not high compared to other foods, but the consumption is very high from infancy to adolescence and the consumption of related dairy products including cheeses increases. The problem of cholesterol content in milk has been raised. In addition, in determining the quality of a conventional milk, only milk fat content is used as a standard, but today, various factors that may lower the quality of milk, such as the presence of antibiotics, bacterial counts, and somatic cell counts, are based on. Among them, the somatic cell count is an important factor to measure the health status of cows and is the first factor to distinguish milk quality. However, no clear alternatives have been made except for antibiotics.

Recently, there have been reports that the cholesterol content of livestock raised by adding chitin or chitosan, which is known as a functional new material of foods, has been reduced by 5%. The use of chitooligosaccharides for lowering cholesterol and lowering somatic cell count in milk has not been reported.

Purpose of the Invention

The present invention solves the problem of using antibiotics by reinforcing the disease resistance of cows in the field of milk and dairy products as described above, lowering the cholesterol content of milk and dairy products, and in particular to lower the somatic cell counts (유) It is aimed at developing feed additives and animal medicine for treating mastitis of cows.

Summary of the Invention

In the milk of the present invention, a cholesterol, somatic cell lowering agent, and cow's mastitis treatment agent are chitooligosaccharides or chitosan obtained from chitin, known as a functional new material of food, chitosan, or low molecular weight polymer, in order to achieve the above object. Chitin, chitosan (molecular weight of less than 10,000) is prepared in liquid or powder form to be added to feed or drinking water to be ingested.

Description of Specific Embodiments of the Invention

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings.

The present inventors apply low molecular weight chitin, chitosan (molecular weight less than 10,000) or chitooligosaccharide to the rearing of cows to maintain the cholesterol level in milk in a short period of time. The present invention has been made by discovering that mastitis does not occur and the number of somatic cells in milk is reduced.

Chitin is a polysaccharide insoluble in water composed of acetylglucosamine or glucosamine as shown in FIG. It is said to be abundant resources on earth after fiber, which generates about 10 billion tons per year in the biological system.

The safety of chitin has already been proved along with its chitooligosaccharides, and it has medical anticancer activity, lowers blood cholesterol levels, suppresses hypertension, controls blood sugar, regulates liver fat, and activates immune activity. It has excellent antibacterial activity and growth activity of enterobacteriaceae, and has been applied to various fields.

Chitooligosaccharide means that the physical property of water is decomposed by decomposing chitin or chitosan by the chemical or enzymatic method such that the polymerization degree of acetylglucosamine or glucosamine is from 2 to 10 or so.

Cholesterol and somatic cell lowering agents of the present invention are prepared by a process for producing low molecular weight chitin, chitosan and chitooligosaccharides.

The low molecular weight chitin, chitosan and chitooligosaccharides are separated and purified into chitin and chitosan by chitin, desalting, deproteinization, and depigmentation of the chitin contained in crabs and shrimp shells as shown in FIG. As shown in FIG. 3, the separated and purified chitin and chitosan are prepared by digesting using chitosan degrading enzyme or chemical treatment.

In the preparation of low-molecular chitin, chitosan and chitooligosaccharides, chitin is made into a colloidal aqueous solution of 5% to 10% concentration and reacted after addition of enzyme at 37 ° C. Heat treatment and filter to inactivate ground enzyme.

Chitosan is dissolved in 1% to 4% of acetic acid, lactic acid, citric acid, malic acid, hydrochloric acid, hydrochloric acid, and ascorbic acid to 5% to 10%, or made into a colloidal aqueous solution. Made by heat treatment and filtration.

EXAMPLE

1) Preparation of Low Molecular Chitin and Chitooligosaccharides from Enzymes by Chitin

The purified chitin 5% suspension was pulverized to a colloidal state by a grinder, separated from bacteria or radiation bacteria, and digested with a purified enzyme to decompose at least 37 hours at 37 ° C. as shown in FIG. A chitin (molecular weight of less than 10,000) or chitin oligosaccharide solution is obtained and insolubles are filtered off.

2) Preparation of Low Molecular Chitosan and Chitooligosaccharides from Enzymes by Chitosan

① Preparation of acetic acid-containing low molecular weight chitosan and chitooligosaccharides

2.5% of acetic acid was added to the purified chitosan 10% suspension, stirred and dissolved, and digested at 37 ° C. for 10 to 24 hours using bacteria or purified enzymes to accommodate low molecular weight chitosan (molecular weight: 10,000) or chitooligosaccharide. And insolubles are filtered off.

② Preparation of Lactic Acid-Containing Low Molecular Weight Chitosan and Chitooligosaccharides

4.5% lactic acid was added to the purified chitosan 10% suspension to stir, dissolve, and digested at 37 ° C. for 10 to 24 hours using bacteria or purified enzymes. Oligosaccharides are obtained and insolubles are removed by filtration.

3) Removal of low molecular weight chitin, chitosan and chitooligosaccharides by chemical treatment

5-10 times concentrated hydrochloric acid was added to purified chitin and chitosan, and then dissolved at 50-80 ° C for 1 to 8 hours while being dissolved in purified water or alcohol to obtain low molecular weight chitin, chitosan (molecular weight: 10,000) or chitooligosaccharide. And insolubles are filtered off.

The low-molecular chitin, chitosan or chitooligosaccharides obtained in Examples 1), 2) and 3) were dissolved in drinking water and fed to dairy cows on a daily basis to consume about 10 grams of milk per day. The milk was inspected. No significant difference in protein or fat content was found in comparison with control, but 12.5mg of cholesterol was detected in 100g of control, but 5.6mg was detected in experimental, and 55% of cholesterol was lowered. As a result of the somatic cell count, milk cows showed milk somatic cell counts by supplying 20g of low molecular weight chitin, chitosan or chitooligosaccharides daily to cows showing initial symptoms of mastitis with more than 500,000 cells per ml. It was determined to have disappeared completely.

In particular, continuous feeding of low-molecular chitin, chitosan, and chitooligosaccharides prevented the development of mastitis without the use of antibiotics, and the effect was maintained even in lowering cholesterol and somatic cell counts in milk.

Effects of the Invention

First, by using low-molecular chitin, chitosan (molecular weight 10,000 or less) or chitooligosaccharides, which are decomposed products of chitin and chitosan, mastitis can be suppressed without using antibiotics, and the health of cows can be maintained and high quality can be achieved by reducing cholesterol and somatic cell count. Milk production is possible.

Second, compared to other raw materials that are safe as natural products and require a large amount of addition, the effect can be obtained at a low concentration of around 0.1%, and it is easy and economical to use them as simple raw materials.

Claims (1)

By using chitin or chitosan obtained from chitin, it is decomposed by chemical or enzymatic method to obtain low molecular weight (molecular weight less than 10,000) chitin, chitosan or chitooligosaccharides, which are 0.1-100% mixed feed formulations or animal drugs. Treatment of mastitis, lowering of somatic cell count and cholesterol lowering agent in milk, characterized in that it is prepared in powder or liquid form.