KR102879640B1 - Aav 벡터 컬럼 정제 방법 - Google Patents

Aav 벡터 컬럼 정제 방법

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Publication number
KR102879640B1
KR102879640B1 KR1020247016903A KR20247016903A KR102879640B1 KR 102879640 B1 KR102879640 B1 KR 102879640B1 KR 1020247016903 A KR1020247016903 A KR 1020247016903A KR 20247016903 A KR20247016903 A KR 20247016903A KR 102879640 B1 KR102879640 B1 KR 102879640B1
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column
nacl
raav vector
aav
raav
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Korean (ko)
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KR20240093848A (ko
Inventor
영훈 오
광 쿠
Original Assignee
스파크 테라퓨틱스, 인코포레이티드
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/34—Size-selective separation, e.g. size-exclusion chromatography; Gel filtration; Permeation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B01D15/362—Cation-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B01D15/363—Anion-exchange
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14151—Methods of production or purification of viral material

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
KR1020247016903A 2017-06-30 2018-06-29 Aav 벡터 컬럼 정제 방법 Active KR102879640B1 (ko)

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US201762527633P 2017-06-30 2017-06-30
US62/527,633 2017-06-30
US201762531744P 2017-07-12 2017-07-12
US62/531,744 2017-07-12
US201762567905P 2017-10-04 2017-10-04
US62/567,905 2017-10-04
PCT/US2018/040430 WO2019006390A1 (en) 2017-06-30 2018-06-29 AAV VECTOR COLUMN PURIFICATION METHOD
KR1020207002118A KR102669561B1 (ko) 2017-06-30 2018-06-29 Aav 벡터 컬럼 정제 방법

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
KR1020207002118A Division KR102669561B1 (ko) 2017-06-30 2018-06-29 Aav 벡터 컬럼 정제 방법

Publications (2)

Publication Number Publication Date
KR20240093848A KR20240093848A (ko) 2024-06-24
KR102879640B1 true KR102879640B1 (ko) 2025-10-30

Family

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KR1020247016903A Active KR102879640B1 (ko) 2017-06-30 2018-06-29 Aav 벡터 컬럼 정제 방법
KR1020207002118A Active KR102669561B1 (ko) 2017-06-30 2018-06-29 Aav 벡터 컬럼 정제 방법

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Country Status (16)

Country Link
US (1) US20210079422A1 (cg-RX-API-DMAC7.html)
EP (1) EP3658250A4 (cg-RX-API-DMAC7.html)
JP (3) JP2020526190A (cg-RX-API-DMAC7.html)
KR (2) KR102879640B1 (cg-RX-API-DMAC7.html)
CN (1) CN111032176A (cg-RX-API-DMAC7.html)
AU (1) AU2018291023B2 (cg-RX-API-DMAC7.html)
CA (1) CA3068622A1 (cg-RX-API-DMAC7.html)
CL (1) CL2019003915A1 (cg-RX-API-DMAC7.html)
CO (1) CO2020000911A2 (cg-RX-API-DMAC7.html)
IL (1) IL271745A (cg-RX-API-DMAC7.html)
MX (2) MX2020000216A (cg-RX-API-DMAC7.html)
MY (1) MY207534A (cg-RX-API-DMAC7.html)
PE (2) PE20251545A1 (cg-RX-API-DMAC7.html)
PH (1) PH12020500044A1 (cg-RX-API-DMAC7.html)
SG (1) SG11201913157RA (cg-RX-API-DMAC7.html)
WO (1) WO2019006390A1 (cg-RX-API-DMAC7.html)

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US20220033782A1 (en) * 2020-07-29 2022-02-03 Pall Corporation Adenovirus-associated viruses separation method
US20220154223A1 (en) 2020-10-15 2022-05-19 Hoffmann-La Roche Inc. Nucleic acid constructs for simultaneous gene activation
WO2022079083A1 (en) 2020-10-15 2022-04-21 F. Hoffmann-La Roche Ag Nucleic acid constructs for va rna transcription
JP7767029B2 (ja) * 2021-05-28 2025-11-11 ダイセン・メンブレン・システムズ株式会社 細胞外小胞の分離精製方法
JP7716233B2 (ja) * 2021-05-28 2025-07-31 ダイセン・メンブレン・システムズ株式会社 微小有用物質の分離精製方法と分離精製装置
BR112023025999A2 (pt) * 2021-06-11 2024-02-27 Spark Therapeutics Inc Métodos de purificação de coluna de vetor de aav
WO2023023541A1 (en) * 2021-08-17 2023-02-23 Ultragenyx Pharmaceutical Inc. Anion-exchange chromatography methods for purification of recombinant adeno-associated viruses
EP4408857A4 (en) * 2021-09-28 2025-11-05 Kashiv Biosciences Llc IMPROVED PROCESS FOR PURIFICATION OF FUSION PROTEIN
CN114250222A (zh) * 2021-12-08 2022-03-29 苏州博腾生物制药有限公司 一种从动物组织中提取aav dna的方法
GB202117844D0 (en) * 2021-12-09 2022-01-26 Oxford Biomedica Ltd Purification method
JP7898711B2 (ja) * 2022-03-24 2026-08-03 国立大学法人 東京大学 ウイルスの精製方法
EP4508241A1 (en) 2022-04-13 2025-02-19 F. Hoffmann-La Roche AG Method for determining aav genomes
EP4529614A1 (en) 2022-05-23 2025-04-02 F. Hoffmann-La Roche AG Raman-based method for the differentiation of aav particle serotype and aav particle loading status
JP2025519228A (ja) 2022-06-03 2025-06-24 エフ． ホフマン－ラ ロシュ アーゲー 組換えａａｖ粒子を生成するための方法
WO2024013239A1 (en) 2022-07-14 2024-01-18 F. Hoffmann-La Roche Ag Method for producing recombinant aav particles
WO2024024337A1 (ja) * 2022-07-28 2024-02-01 株式会社ダイセル 微小有用物質を含む液の精製濃縮装置及びそれを用いた微小有用物質を含む精製濃縮液の製造方法
JP2025530294A (ja) 2022-09-12 2025-09-11 エフ． ホフマン－ラ ロシュ アーゲー 完全なａａｖ粒子と空のａａｖ粒子を分離するための方法
EP4385618A1 (en) 2022-12-16 2024-06-19 Chiral Technologies Europe SAS Composite material for bioseparations
AU2024216670A1 (en) 2023-02-07 2025-07-31 F. Hoffmann-La Roche Ag Method for the detection of anti-aav particle antibodies
WO2024194280A1 (en) 2023-03-21 2024-09-26 F. Hoffmann-La Roche Ag Method for the production of recombinant aav particle preparations
EP4694994A1 (en) * 2023-04-12 2026-02-18 Cytiva BioProcess R&D AB A chromatography system, use thereof, and a method for separating adeno-associated capsids
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WO2025217029A1 (en) * 2024-04-07 2025-10-16 Spark Therapeutics, Inc. Compositions & methods for adeno-associated virus capsid variant purification
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US20260022401A1 (en) 2024-07-22 2026-01-22 Hoffmann-La Roche Inc. Novel AAV rep ORFs and Rep polypeptides
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Also Published As

Publication number Publication date
MX2024007103A (es) 2024-06-24
PE20251545A1 (es) 2025-06-05
MY207534A (en) 2025-03-03
JP2025038906A (ja) 2025-03-19
RU2020103743A (ru) 2021-07-30
KR20240093848A (ko) 2024-06-24
PH12020500044A1 (en) 2020-09-14
CO2020000911A2 (es) 2020-06-19
WO2019006390A1 (en) 2019-01-03
RU2020103743A3 (cg-RX-API-DMAC7.html) 2021-09-30
CN111032176A (zh) 2020-04-17
KR102669561B1 (ko) 2024-05-24
EP3658250A4 (en) 2021-10-27
AU2018291023B2 (en) 2023-11-02
PE20200737A1 (es) 2020-07-23
BR112019028299A2 (pt) 2020-07-14
US20210079422A1 (en) 2021-03-18
JP2023029832A (ja) 2023-03-07
CL2019003915A1 (es) 2020-06-19
JP2020526190A (ja) 2020-08-31
IL271745A (en) 2020-02-27
EP3658250A1 (en) 2020-06-03
CA3068622A1 (en) 2019-01-03
AU2018291023A1 (en) 2020-02-06
SG11201913157RA (en) 2020-01-30
KR20200040749A (ko) 2020-04-20
MX2020000216A (es) 2020-09-03

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