JP2020526190A - Ａａｖベクターのカラム精製方法 - Google Patents

Ａａｖベクターのカラム精製方法 Download PDF

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Publication number
JP2020526190A
JP2020526190A JP2019572175A JP2019572175A JP2020526190A JP 2020526190 A JP2020526190 A JP 2020526190A JP 2019572175 A JP2019572175 A JP 2019572175A JP 2019572175 A JP2019572175 A JP 2019572175A JP 2020526190 A JP2020526190 A JP 2020526190A
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JP
Japan
Prior art keywords
column
column eluate
vector particles
raav vector
lysate
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Pending
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JP2019572175A
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English (en)
Japanese (ja)
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JP2020526190A5 (cg-RX-API-DMAC7.html
Inventor
ヨンフン オウ，
ヨンフン オウ，
グァン チュウ，
グァン チュウ，
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Spark Therapeutics Inc
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Spark Therapeutics Inc
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Application filed by Spark Therapeutics Inc filed Critical Spark Therapeutics Inc
Publication of JP2020526190A publication Critical patent/JP2020526190A/ja
Publication of JP2020526190A5 publication Critical patent/JP2020526190A5/ja
Priority to JP2022177960A priority Critical patent/JP2023029832A/ja
Priority to JP2024211621A priority patent/JP2025038906A/ja
Pending legal-status Critical Current

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/34—Size-selective separation, e.g. size-exclusion chromatography; Gel filtration; Permeation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B01D15/362—Cation-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B01D15/363—Anion-exchange
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14151—Methods of production or purification of viral material

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
JP2019572175A 2017-06-30 2018-06-29 Ａａｖベクターのカラム精製方法 Pending JP2020526190A (ja)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP2022177960A JP2023029832A (ja) 2017-06-30 2022-11-07 Ａａｖベクターのカラム精製方法
JP2024211621A JP2025038906A (ja) 2017-06-30 2024-12-04 Ａａｖベクターのカラム精製方法

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201762527633P 2017-06-30 2017-06-30
US62/527,633 2017-06-30
US201762531744P 2017-07-12 2017-07-12
US62/531,744 2017-07-12
US201762567905P 2017-10-04 2017-10-04
US62/567,905 2017-10-04
PCT/US2018/040430 WO2019006390A1 (en) 2017-06-30 2018-06-29 AAV VECTOR COLUMN PURIFICATION METHOD

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2022177960A Division JP2023029832A (ja) 2017-06-30 2022-11-07 Ａａｖベクターのカラム精製方法

Publications (2)

Publication Number Publication Date
JP2020526190A true JP2020526190A (ja) 2020-08-31
JP2020526190A5 JP2020526190A5 (cg-RX-API-DMAC7.html) 2021-07-26

Family

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Family Applications (3)

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JP2019572175A Pending JP2020526190A (ja) 2017-06-30 2018-06-29 Ａａｖベクターのカラム精製方法
JP2022177960A Pending JP2023029832A (ja) 2017-06-30 2022-11-07 Ａａｖベクターのカラム精製方法
JP2024211621A Pending JP2025038906A (ja) 2017-06-30 2024-12-04 Ａａｖベクターのカラム精製方法

Family Applications After (2)

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JP2022177960A Pending JP2023029832A (ja) 2017-06-30 2022-11-07 Ａａｖベクターのカラム精製方法
JP2024211621A Pending JP2025038906A (ja) 2017-06-30 2024-12-04 Ａａｖベクターのカラム精製方法

Country Status (16)

Country Link
US (1) US20210079422A1 (cg-RX-API-DMAC7.html)
EP (1) EP3658250A4 (cg-RX-API-DMAC7.html)
JP (3) JP2020526190A (cg-RX-API-DMAC7.html)
KR (2) KR102879640B1 (cg-RX-API-DMAC7.html)
CN (1) CN111032176A (cg-RX-API-DMAC7.html)
AU (1) AU2018291023B2 (cg-RX-API-DMAC7.html)
CA (1) CA3068622A1 (cg-RX-API-DMAC7.html)
CL (1) CL2019003915A1 (cg-RX-API-DMAC7.html)
CO (1) CO2020000911A2 (cg-RX-API-DMAC7.html)
IL (1) IL271745A (cg-RX-API-DMAC7.html)
MX (2) MX2020000216A (cg-RX-API-DMAC7.html)
MY (1) MY207534A (cg-RX-API-DMAC7.html)
PE (2) PE20251545A1 (cg-RX-API-DMAC7.html)
PH (1) PH12020500044A1 (cg-RX-API-DMAC7.html)
SG (1) SG11201913157RA (cg-RX-API-DMAC7.html)
WO (1) WO2019006390A1 (cg-RX-API-DMAC7.html)

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WO2022250036A1 (ja) * 2021-05-28 2022-12-01 ダイセン・メンブレン・システムズ株式会社 微小有用物質の分離精製方法と分離精製装置
WO2022250035A1 (ja) * 2021-05-28 2022-12-01 ダイセン・メンブレン・システムズ株式会社 細胞外小胞の分離精製方法
WO2023182428A1 (ja) * 2022-03-24 2023-09-28 国立大学法人 東京大学 ウイルスの精製方法
WO2024024337A1 (ja) * 2022-07-28 2024-02-01 株式会社ダイセル 微小有用物質を含む液の精製濃縮装置及びそれを用いた微小有用物質を含む精製濃縮液の製造方法
JP2024525142A (ja) * 2021-06-11 2024-07-10 スパーク セラピューティクス インコーポレイテッド Ａａｖベクターカラム精製方法
JP2024532133A (ja) * 2021-08-17 2024-09-05 ウルトラジェニックス ファーマシューティカル インコーポレイテッド 組換えアデノ随伴ウイルスの精製のためのアニオン交換クロマトグラフィー方法

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US11008561B2 (en) 2014-06-30 2021-05-18 Bioverativ Therapeutics Inc. Optimized factor IX gene
GB201508026D0 (en) 2015-05-11 2015-06-24 Ucl Business Plc Capsid
ES2965341T3 (es) 2019-04-11 2024-04-12 Regenxbio Inc Métodos de cromatografía de exclusión por tamaño para la caracterización de composiciones de virus adenoasociados recombinantes
JP7803014B2 (ja) 2019-09-30 2026-01-21 バイオベラティブ セラピューティクス インコーポレイテッド レンチウイルスベクター製剤
AU2021262609A1 (en) 2020-05-01 2022-12-22 Kashiv Biosciences, Llc An improved process of purification of protein
EP3919613B1 (en) * 2020-06-05 2024-08-07 Sartorius BIA Separations d.o.o. Enhanced purification of adeno-associated virus to more effectively remove contaminating dna
JP2023537565A (ja) * 2020-06-24 2023-09-04 バイオベラティブ セラピューティクス インコーポレイテッド タンパク質を発現するように改変されたレンチウイルスベクターの調製物から遊離第ｖｉｉｉ因子を除去する方法
US20220033782A1 (en) * 2020-07-29 2022-02-03 Pall Corporation Adenovirus-associated viruses separation method
US20220154223A1 (en) 2020-10-15 2022-05-19 Hoffmann-La Roche Inc. Nucleic acid constructs for simultaneous gene activation
WO2022079083A1 (en) 2020-10-15 2022-04-21 F. Hoffmann-La Roche Ag Nucleic acid constructs for va rna transcription
EP4408857A4 (en) * 2021-09-28 2025-11-05 Kashiv Biosciences Llc IMPROVED PROCESS FOR PURIFICATION OF FUSION PROTEIN
CN114250222A (zh) * 2021-12-08 2022-03-29 苏州博腾生物制药有限公司 一种从动物组织中提取aav dna的方法
GB202117844D0 (en) * 2021-12-09 2022-01-26 Oxford Biomedica Ltd Purification method
EP4508241A1 (en) 2022-04-13 2025-02-19 F. Hoffmann-La Roche AG Method for determining aav genomes
EP4529614A1 (en) 2022-05-23 2025-04-02 F. Hoffmann-La Roche AG Raman-based method for the differentiation of aav particle serotype and aav particle loading status
JP2025519228A (ja) 2022-06-03 2025-06-24 エフ． ホフマン－ラ ロシュ アーゲー 組換えａａｖ粒子を生成するための方法
WO2024013239A1 (en) 2022-07-14 2024-01-18 F. Hoffmann-La Roche Ag Method for producing recombinant aav particles
JP2025530294A (ja) 2022-09-12 2025-09-11 エフ． ホフマン－ラ ロシュ アーゲー 完全なａａｖ粒子と空のａａｖ粒子を分離するための方法
EP4385618A1 (en) 2022-12-16 2024-06-19 Chiral Technologies Europe SAS Composite material for bioseparations
AU2024216670A1 (en) 2023-02-07 2025-07-31 F. Hoffmann-La Roche Ag Method for the detection of anti-aav particle antibodies
WO2024194280A1 (en) 2023-03-21 2024-09-26 F. Hoffmann-La Roche Ag Method for the production of recombinant aav particle preparations
EP4694994A1 (en) * 2023-04-12 2026-02-18 Cytiva BioProcess R&D AB A chromatography system, use thereof, and a method for separating adeno-associated capsids
AU2025218748A1 (en) 2024-02-09 2026-08-06 F. Hoffmann-La Roche Ag Method for producing recombinant adeno-associated viral particles
WO2025217029A1 (en) * 2024-04-07 2025-10-16 Spark Therapeutics, Inc. Compositions & methods for adeno-associated virus capsid variant purification
WO2025252480A1 (en) 2024-06-07 2025-12-11 F. Hoffmann-La Roche Ag Method for purifying plasmid dna
US20260022401A1 (en) 2024-07-22 2026-01-22 Hoffmann-La Roche Inc. Novel AAV rep ORFs and Rep polypeptides
WO2026037757A1 (en) 2024-08-13 2026-02-19 F. Hoffmann-La Roche Ag Modified aav particles
WO2026132139A1 (en) 2024-12-20 2026-06-25 F. Hoffmann-La Roche Ag Purification process for viral vectors
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JP2013517798A (ja) * 2010-01-28 2013-05-20 ザ チルドレンズ ホスピタル オブ フィラデルフィア ウイルスベクター精製における拡張可能な製造プラットフォームおよび遺伝子治療における使用のための高純度ウイルスベクター
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Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022250036A1 (ja) * 2021-05-28 2022-12-01 ダイセン・メンブレン・システムズ株式会社 微小有用物質の分離精製方法と分離精製装置
WO2022250035A1 (ja) * 2021-05-28 2022-12-01 ダイセン・メンブレン・システムズ株式会社 細胞外小胞の分離精製方法
JP2022182361A (ja) * 2021-05-28 2022-12-08 ダイセン・メンブレン・システムズ株式会社 微小有用物質の分離精製方法と分離精製装置
JP2022182360A (ja) * 2021-05-28 2022-12-08 ダイセン・メンブレン・システムズ株式会社 細胞外小胞の分離精製方法
JP7716233B2 (ja) 2021-05-28 2025-07-31 ダイセン・メンブレン・システムズ株式会社 微小有用物質の分離精製方法と分離精製装置
JP7767029B2 (ja) 2021-05-28 2025-11-11 ダイセン・メンブレン・システムズ株式会社 細胞外小胞の分離精製方法
JP2024525142A (ja) * 2021-06-11 2024-07-10 スパーク セラピューティクス インコーポレイテッド Ａａｖベクターカラム精製方法
JP2024532133A (ja) * 2021-08-17 2024-09-05 ウルトラジェニックス ファーマシューティカル インコーポレイテッド 組換えアデノ随伴ウイルスの精製のためのアニオン交換クロマトグラフィー方法
WO2023182428A1 (ja) * 2022-03-24 2023-09-28 国立大学法人 東京大学 ウイルスの精製方法
JP2023141423A (ja) * 2022-03-24 2023-10-05 国立大学法人 東京大学 ウイルスの精製方法
JP7898711B2 (ja) 2022-03-24 2026-08-03 国立大学法人 東京大学 ウイルスの精製方法
WO2024024337A1 (ja) * 2022-07-28 2024-02-01 株式会社ダイセル 微小有用物質を含む液の精製濃縮装置及びそれを用いた微小有用物質を含む精製濃縮液の製造方法

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MX2024007103A (es) 2024-06-24
PE20251545A1 (es) 2025-06-05
MY207534A (en) 2025-03-03
JP2025038906A (ja) 2025-03-19
RU2020103743A (ru) 2021-07-30
KR20240093848A (ko) 2024-06-24
PH12020500044A1 (en) 2020-09-14
CO2020000911A2 (es) 2020-06-19
WO2019006390A1 (en) 2019-01-03
KR102879640B1 (ko) 2025-10-30
RU2020103743A3 (cg-RX-API-DMAC7.html) 2021-09-30
CN111032176A (zh) 2020-04-17
KR102669561B1 (ko) 2024-05-24
EP3658250A4 (en) 2021-10-27
AU2018291023B2 (en) 2023-11-02
PE20200737A1 (es) 2020-07-23
BR112019028299A2 (pt) 2020-07-14
US20210079422A1 (en) 2021-03-18
JP2023029832A (ja) 2023-03-07
CL2019003915A1 (es) 2020-06-19
IL271745A (en) 2020-02-27
EP3658250A1 (en) 2020-06-03
CA3068622A1 (en) 2019-01-03
AU2018291023A1 (en) 2020-02-06
SG11201913157RA (en) 2020-01-30
KR20200040749A (ko) 2020-04-20
MX2020000216A (es) 2020-09-03

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