KR102789904B1 - 게놈을 변형시키기 위한 조성물 및 방법 - Google Patents
게놈을 변형시키기 위한 조성물 및 방법 Download PDFInfo
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- KR102789904B1 KR102789904B1 KR1020247003280A KR20247003280A KR102789904B1 KR 102789904 B1 KR102789904 B1 KR 102789904B1 KR 1020247003280 A KR1020247003280 A KR 1020247003280A KR 20247003280 A KR20247003280 A KR 20247003280A KR 102789904 B1 KR102789904 B1 KR 102789904B1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/825—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving pigment biosynthesis
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
- C12N15/8269—Photosynthesis
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/10—Fusion polypeptide containing a localisation/targetting motif containing a tag for extracellular membrane crossing, e.g. TAT or VP22
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2510/00—Genetically modified cells
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/22—Vectors comprising a coding region that has been codon optimised for expression in a respective host
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- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Crystallography & Structural Chemistry (AREA)
- Nutrition Science (AREA)
- Physiology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020257010200A KR20250050118A (ko) | 2017-08-09 | 2018-08-08 | 게놈을 변형시키기 위한 조성물 및 방법 |
Applications Claiming Priority (10)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201762542983P | 2017-08-09 | 2017-08-09 | |
| US62/542,983 | 2017-08-09 | ||
| US201762551958P | 2017-08-30 | 2017-08-30 | |
| US62/551,958 | 2017-08-30 | ||
| US201762565255P | 2017-09-29 | 2017-09-29 | |
| US62/565,255 | 2017-09-29 | ||
| US201762599226P | 2017-12-15 | 2017-12-15 | |
| US62/599,226 | 2017-12-15 | ||
| PCT/IB2018/055972 WO2019030695A1 (en) | 2017-08-09 | 2018-08-08 | Compositions and methods for modifying genomes |
| KR1020207006387A KR102631985B1 (ko) | 2017-08-09 | 2018-08-08 | 게놈을 변형시키기 위한 조성물 및 방법 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020207006387A Division KR102631985B1 (ko) | 2017-08-09 | 2018-08-08 | 게놈을 변형시키기 위한 조성물 및 방법 |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020257010200A Division KR20250050118A (ko) | 2017-08-09 | 2018-08-08 | 게놈을 변형시키기 위한 조성물 및 방법 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| KR20240017981A KR20240017981A (ko) | 2024-02-08 |
| KR102789904B1 true KR102789904B1 (ko) | 2025-04-03 |
Family
ID=63592761
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020247003280A Active KR102789904B1 (ko) | 2017-08-09 | 2018-08-08 | 게놈을 변형시키기 위한 조성물 및 방법 |
| KR1020207006387A Active KR102631985B1 (ko) | 2017-08-09 | 2018-08-08 | 게놈을 변형시키기 위한 조성물 및 방법 |
| KR1020257010200A Pending KR20250050118A (ko) | 2017-08-09 | 2018-08-08 | 게놈을 변형시키기 위한 조성물 및 방법 |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020207006387A Active KR102631985B1 (ko) | 2017-08-09 | 2018-08-08 | 게놈을 변형시키기 위한 조성물 및 방법 |
| KR1020257010200A Pending KR20250050118A (ko) | 2017-08-09 | 2018-08-08 | 게놈을 변형시키기 위한 조성물 및 방법 |
Country Status (11)
| Country | Link |
|---|---|
| US (6) | US10316324B2 (https=) |
| EP (3) | EP3665279B1 (https=) |
| JP (3) | JP7355730B2 (https=) |
| KR (3) | KR102789904B1 (https=) |
| CN (2) | CN111373040B (https=) |
| AU (2) | AU2018315731B2 (https=) |
| BR (1) | BR112020002647A2 (https=) |
| CA (2) | CA3072312C (https=) |
| ES (1) | ES2960390T3 (https=) |
| IL (2) | IL310452A (https=) |
| WO (1) | WO2019030695A1 (https=) |
Families Citing this family (29)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10337051B2 (en) | 2016-06-16 | 2019-07-02 | The Regents Of The University Of California | Methods and compositions for detecting a target RNA |
| BR112019006388A2 (pt) | 2016-09-30 | 2019-06-25 | Univ California | enzimas modificadoras de ácido nucleico guiadas por rna e métodos de uso das mesmas |
| MX2019003674A (es) | 2016-09-30 | 2021-01-08 | Univ California | Enzimas modificadoras de ácido nucleico guiadas por arn y métodos de uso de estas. |
| EP3665279B1 (en) | 2017-08-09 | 2023-07-19 | Benson Hill, Inc. | Compositions and methods for modifying genomes |
| US20210054404A1 (en) | 2017-08-22 | 2021-02-25 | Napigen, Inc. | Organelle genome modification using polynucleotide guided endonuclease |
| WO2019089804A1 (en) | 2017-11-01 | 2019-05-09 | The Regents Of The University Of California | Casy compositions and methods of use |
| US11970719B2 (en) | 2017-11-01 | 2024-04-30 | The Regents Of The University Of California | Class 2 CRISPR/Cas compositions and methods of use |
| AU2018358051B2 (en) * | 2017-11-01 | 2025-01-09 | The Regents Of The University Of California | CasZ compositions and methods of use |
| EP3830301B1 (en) | 2018-08-01 | 2024-05-22 | Mammoth Biosciences, Inc. | Programmable nuclease compositions and methods of use thereof |
| US11407995B1 (en) * | 2018-10-26 | 2022-08-09 | Inari Agriculture Technology, Inc. | RNA-guided nucleases and DNA binding proteins |
| EP3931313A2 (en) | 2019-01-04 | 2022-01-05 | Mammoth Biosciences, Inc. | Programmable nuclease improvements and compositions and methods for nucleic acid amplification and detection |
| US20230002761A1 (en) * | 2019-11-19 | 2023-01-05 | Benson Hill, Inc. | Anti-bacterial crispr compositions and methods |
| CN111100868B (zh) * | 2019-12-17 | 2021-05-25 | 南京林业大学 | 美洲黑杨的促雌基因ferr和抑雌基因ferr-r及其应用 |
| EP3878958A1 (en) * | 2020-03-11 | 2021-09-15 | B.R.A.I.N. Biotechnology Research And Information Network AG | Crispr-cas nucleases from cpr-enriched metagenome |
| US20230212612A1 (en) * | 2020-05-28 | 2023-07-06 | Shanghaitech University | Genome editing system and method |
| CN113930411A (zh) * | 2020-06-29 | 2022-01-14 | 中国农业大学 | 新型CRISPR-Cas12M酶和系统 |
| CN113930413B (zh) * | 2020-06-29 | 2024-12-27 | 中国农业大学 | 新型CRISPR-Cas12j.23酶和系统 |
| EP3943600A1 (en) * | 2020-07-21 | 2022-01-26 | B.R.A.I.N. Biotechnology Research And Information Network AG | Novel, non-naturally occurring crispr-cas nucleases for genome editing |
| EP4274890A4 (en) * | 2021-01-07 | 2024-11-27 | Inscripta, Inc. | MAD-NUCLEASES |
| US20250277199A1 (en) | 2021-03-02 | 2025-09-04 | BRAIN Biotech AG | Novel crispr-cas nucleases from metagenomes |
| US20220389418A1 (en) | 2021-06-01 | 2022-12-08 | Helmholtz-Institut Für Rna-Basierte Nfektionsforschung (Hzi) | Rna-guided cas nucleases and uses thereof in diagnostics and therapy |
| EP4215611A1 (en) | 2022-01-19 | 2023-07-26 | BRAIN Biotech AG | Modification of the genome of a filamentous fungus |
| CA3248677A1 (en) * | 2022-01-19 | 2023-07-27 | BRAIN Biotech AG | CELL DEPLEASE BY CRISPR NUCLEASE |
| EP4215612A1 (en) * | 2022-01-20 | 2023-07-26 | Sartorius Stedim Cellca GmbH | Genome editing with crispr/cas nucleases comprising collateral activity |
| CN115588463B (zh) * | 2022-10-26 | 2025-08-08 | 杭州师范大学 | 基于深度学习挖掘蛋白质相互作用类型的预测方法 |
| EP4361265A1 (en) * | 2022-10-27 | 2024-05-01 | BRAIN Biotech AG | Optimization of editing efficacy of crispr nucleases with collateral activity |
| WO2024146916A1 (en) * | 2023-01-04 | 2024-07-11 | BRAIN Biotech AG | Activated bec nucleases for degrading nucleic acid molecules |
| US20260085302A1 (en) | 2024-09-20 | 2026-03-26 | Confluence Genetics, Llc | Compositions and methods for controlling plant pests |
| CN119246486B (zh) * | 2024-12-06 | 2025-04-01 | 南京师范大学 | 一种Cas蛋白在食用油鉴别中的应用 |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103981215A (zh) | 2014-05-23 | 2014-08-13 | 安徽省农业科学院水稻研究所 | 一种用于基因工程的骨干质粒载体及应用 |
| US20170114351A1 (en) | 2014-06-12 | 2017-04-27 | King Abdullah University Of Science And Technology | TARGETED VIRAL-MEDIATED PLANT GENOME EDITING USING CRISPR /Cas9 |
| WO2017141173A2 (en) * | 2016-02-15 | 2017-08-24 | Benson Hill Biosystems, Inc. | Compositions and methods for modifying genomes |
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