CN111373040B - 修饰基因组的组合物和方法 - Google Patents

修饰基因组的组合物和方法 Download PDF

Info

Publication number
CN111373040B
CN111373040B CN201880065634.1A CN201880065634A CN111373040B CN 111373040 B CN111373040 B CN 111373040B CN 201880065634 A CN201880065634 A CN 201880065634A CN 111373040 B CN111373040 B CN 111373040B
Authority
CN
China
Prior art keywords
cms1
sequence
polypeptide
dna
protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201880065634.1A
Other languages
English (en)
Chinese (zh)
Other versions
CN111373040A (zh
Inventor
M·贝其曼
B·N·格雷
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ricetec Inc
Original Assignee
Benson Hill Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Benson Hill Inc filed Critical Benson Hill Inc
Priority to CN202410204271.2A priority Critical patent/CN118497198A/zh
Publication of CN111373040A publication Critical patent/CN111373040A/zh
Application granted granted Critical
Publication of CN111373040B publication Critical patent/CN111373040B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/825—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving pigment biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
    • C12N15/8269—Photosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/10—Fusion polypeptide containing a localisation/targetting motif containing a tag for extracellular membrane crossing, e.g. TAT or VP22
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/22—Vectors comprising a coding region that has been codon optimised for expression in a respective host

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Nutrition Science (AREA)
  • Physiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201880065634.1A 2017-08-09 2018-08-08 修饰基因组的组合物和方法 Active CN111373040B (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202410204271.2A CN118497198A (zh) 2017-08-09 2018-08-08 修饰基因组的组合物和方法

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US201762542983P 2017-08-09 2017-08-09
US62/542,983 2017-08-09
US201762551958P 2017-08-30 2017-08-30
US62/551,958 2017-08-30
US201762565255P 2017-09-29 2017-09-29
US62/565,255 2017-09-29
US201762599226P 2017-12-15 2017-12-15
US62/599,226 2017-12-15
PCT/IB2018/055972 WO2019030695A1 (en) 2017-08-09 2018-08-08 Compositions and methods for modifying genomes

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CN202410204271.2A Division CN118497198A (zh) 2017-08-09 2018-08-08 修饰基因组的组合物和方法

Publications (2)

Publication Number Publication Date
CN111373040A CN111373040A (zh) 2020-07-03
CN111373040B true CN111373040B (zh) 2024-07-12

Family

ID=63592761

Family Applications (2)

Application Number Title Priority Date Filing Date
CN201880065634.1A Active CN111373040B (zh) 2017-08-09 2018-08-08 修饰基因组的组合物和方法
CN202410204271.2A Pending CN118497198A (zh) 2017-08-09 2018-08-08 修饰基因组的组合物和方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
CN202410204271.2A Pending CN118497198A (zh) 2017-08-09 2018-08-08 修饰基因组的组合物和方法

Country Status (11)

Country Link
US (6) US10316324B2 (https=)
EP (3) EP3665279B1 (https=)
JP (3) JP7355730B2 (https=)
KR (3) KR102789904B1 (https=)
CN (2) CN111373040B (https=)
AU (2) AU2018315731B2 (https=)
BR (1) BR112020002647A2 (https=)
CA (2) CA3072312C (https=)
ES (1) ES2960390T3 (https=)
IL (2) IL310452A (https=)
WO (1) WO2019030695A1 (https=)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10337051B2 (en) 2016-06-16 2019-07-02 The Regents Of The University Of California Methods and compositions for detecting a target RNA
BR112019006388A2 (pt) 2016-09-30 2019-06-25 Univ California enzimas modificadoras de ácido nucleico guiadas por rna e métodos de uso das mesmas
MX2019003674A (es) 2016-09-30 2021-01-08 Univ California Enzimas modificadoras de ácido nucleico guiadas por arn y métodos de uso de estas.
EP3665279B1 (en) 2017-08-09 2023-07-19 Benson Hill, Inc. Compositions and methods for modifying genomes
US20210054404A1 (en) 2017-08-22 2021-02-25 Napigen, Inc. Organelle genome modification using polynucleotide guided endonuclease
WO2019089804A1 (en) 2017-11-01 2019-05-09 The Regents Of The University Of California Casy compositions and methods of use
US11970719B2 (en) 2017-11-01 2024-04-30 The Regents Of The University Of California Class 2 CRISPR/Cas compositions and methods of use
AU2018358051B2 (en) * 2017-11-01 2025-01-09 The Regents Of The University Of California CasZ compositions and methods of use
EP3830301B1 (en) 2018-08-01 2024-05-22 Mammoth Biosciences, Inc. Programmable nuclease compositions and methods of use thereof
US11407995B1 (en) * 2018-10-26 2022-08-09 Inari Agriculture Technology, Inc. RNA-guided nucleases and DNA binding proteins
EP3931313A2 (en) 2019-01-04 2022-01-05 Mammoth Biosciences, Inc. Programmable nuclease improvements and compositions and methods for nucleic acid amplification and detection
US20230002761A1 (en) * 2019-11-19 2023-01-05 Benson Hill, Inc. Anti-bacterial crispr compositions and methods
CN111100868B (zh) * 2019-12-17 2021-05-25 南京林业大学 美洲黑杨的促雌基因ferr和抑雌基因ferr-r及其应用
EP3878958A1 (en) * 2020-03-11 2021-09-15 B.R.A.I.N. Biotechnology Research And Information Network AG Crispr-cas nucleases from cpr-enriched metagenome
US20230212612A1 (en) * 2020-05-28 2023-07-06 Shanghaitech University Genome editing system and method
CN113930411A (zh) * 2020-06-29 2022-01-14 中国农业大学 新型CRISPR-Cas12M酶和系统
CN113930413B (zh) * 2020-06-29 2024-12-27 中国农业大学 新型CRISPR-Cas12j.23酶和系统
EP3943600A1 (en) * 2020-07-21 2022-01-26 B.R.A.I.N. Biotechnology Research And Information Network AG Novel, non-naturally occurring crispr-cas nucleases for genome editing
EP4274890A4 (en) * 2021-01-07 2024-11-27 Inscripta, Inc. MAD-NUCLEASES
US20250277199A1 (en) 2021-03-02 2025-09-04 BRAIN Biotech AG Novel crispr-cas nucleases from metagenomes
US20220389418A1 (en) 2021-06-01 2022-12-08 Helmholtz-Institut Für Rna-Basierte Nfektionsforschung (Hzi) Rna-guided cas nucleases and uses thereof in diagnostics and therapy
EP4215611A1 (en) 2022-01-19 2023-07-26 BRAIN Biotech AG Modification of the genome of a filamentous fungus
CA3248677A1 (en) * 2022-01-19 2023-07-27 BRAIN Biotech AG CELL DEPLEASE BY CRISPR NUCLEASE
EP4215612A1 (en) * 2022-01-20 2023-07-26 Sartorius Stedim Cellca GmbH Genome editing with crispr/cas nucleases comprising collateral activity
CN115588463B (zh) * 2022-10-26 2025-08-08 杭州师范大学 基于深度学习挖掘蛋白质相互作用类型的预测方法
EP4361265A1 (en) * 2022-10-27 2024-05-01 BRAIN Biotech AG Optimization of editing efficacy of crispr nucleases with collateral activity
WO2024146916A1 (en) * 2023-01-04 2024-07-11 BRAIN Biotech AG Activated bec nucleases for degrading nucleic acid molecules
US20260085302A1 (en) 2024-09-20 2026-03-26 Confluence Genetics, Llc Compositions and methods for controlling plant pests
CN119246486B (zh) * 2024-12-06 2025-04-01 南京师范大学 一种Cas蛋白在食用油鉴别中的应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105142669A (zh) * 2012-12-06 2015-12-09 西格马-奥尔德里奇有限责任公司 基于crispr的基因组修饰和调控
CN109312316A (zh) * 2016-02-15 2019-02-05 本森希尔生物系统股份有限公司 修饰基因组的组合物和方法

Family Cites Families (75)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4945050A (en) 1984-11-13 1990-07-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
US5569597A (en) 1985-05-13 1996-10-29 Ciba Geigy Corp. Methods of inserting viral DNA into plant material
US5268463A (en) 1986-11-11 1993-12-07 Jefferson Richard A Plant promoter α-glucuronidase gene construct
US5608142A (en) 1986-12-03 1997-03-04 Agracetus, Inc. Insecticidal cotton plants
US5990387A (en) 1988-06-10 1999-11-23 Pioneer Hi-Bred International, Inc. Stable transformation of plant cells
US6015891A (en) 1988-09-09 2000-01-18 Mycogen Plant Science, Inc. Synthetic insecticidal crystal protein gene having a modified frequency of codon usage
US5023179A (en) 1988-11-14 1991-06-11 Eric Lam Promoter enhancer element for gene expression in plant roots
US5110732A (en) 1989-03-14 1992-05-05 The Rockefeller University Selective gene expression in plants
EP0388186A1 (en) 1989-03-17 1990-09-19 E.I. Du Pont De Nemours And Company External regulation of gene expression
US5240855A (en) 1989-05-12 1993-08-31 Pioneer Hi-Bred International, Inc. Particle gun
US5879918A (en) 1989-05-12 1999-03-09 Pioneer Hi-Bred International, Inc. Pretreatment of microprojectiles prior to using in a particle gun
US5322783A (en) 1989-10-17 1994-06-21 Pioneer Hi-Bred International, Inc. Soybean transformation by microparticle bombardment
ATE225853T1 (de) 1990-04-12 2002-10-15 Syngenta Participations Ag Gewebe-spezifische promotoren
US5498830A (en) 1990-06-18 1996-03-12 Monsanto Company Decreased oil content in plant seeds
US5932782A (en) 1990-11-14 1999-08-03 Pioneer Hi-Bred International, Inc. Plant transformation method using agrobacterium species adhered to microprojectiles
US5459252A (en) 1991-01-31 1995-10-17 North Carolina State University Root specific gene promoter
US5399680A (en) 1991-05-22 1995-03-21 The Salk Institute For Biological Studies Rice chitinase promoter
US5604121A (en) 1991-08-27 1997-02-18 Agricultural Genetics Company Limited Proteins with insecticidal properties against homopteran insects and their use in plant protection
ATE276373T1 (de) 1991-10-04 2004-10-15 Univ North Carolina State Pathogenresistente transgene pflanzen
US5324646A (en) 1992-01-06 1994-06-28 Pioneer Hi-Bred International, Inc. Methods of regeneration of Medicago sativa and expressing foreign DNA in same
US5401836A (en) 1992-07-16 1995-03-28 Pioneer Hi-Bre International, Inc. Brassica regulatory sequence for root-specific or root-abundant gene expression
JP2952041B2 (ja) 1992-07-27 1999-09-20 パイオニア ハイ−ブレッド インターナショナル，インコーポレイテッド 培養ダイズ細胞のａｇｒｏｂａｃｔｅｒｉｕｍ媒介形質転換の改良法
AU5676394A (en) 1992-11-20 1994-06-22 Agracetus, Inc. Transgenic cotton plants producing heterologous bioplastic
CA2161881C (en) 1993-01-13 2001-03-27 A. Gururaj Rao High lysine derivatives of alpha-hordothionin
US5583210A (en) 1993-03-18 1996-12-10 Pioneer Hi-Bred International, Inc. Methods and compositions for controlling plant development
US5789156A (en) 1993-06-14 1998-08-04 Basf Ag Tetracycline-regulated transcriptional inhibitors
US5814618A (en) 1993-06-14 1998-09-29 Basf Aktiengesellschaft Methods for regulating gene expression
US5470353A (en) 1993-10-20 1995-11-28 Hollister Incorporated Post-operative thermal blanket
US5633363A (en) 1994-06-03 1997-05-27 Iowa State University, Research Foundation In Root preferential promoter
US5736369A (en) 1994-07-29 1998-04-07 Pioneer Hi-Bred International, Inc. Method for producing transgenic cereal plants
US5608144A (en) 1994-08-12 1997-03-04 Dna Plant Technology Corp. Plant group 2 promoters and uses thereof
US5981833A (en) * 1994-11-29 1999-11-09 Iowa State University Research Foundation, Inc. Nuclear restorer genes for hybrid seed production
EP0828835A1 (en) 1995-06-02 1998-03-18 Pioneer Hi-Bred International, Inc. HIGH THREONINE DERIVATIVES OF $g(a)-HORDOTHIONIN
PL323635A1 (en) 1995-06-02 1998-04-14 Pioneer Hi Bred Int Derivatives of alpha-hordothionine of high methionine content
US5837876A (en) 1995-07-28 1998-11-17 North Carolina State University Root cortex specific gene promoter
US5703049A (en) 1996-02-29 1997-12-30 Pioneer Hi-Bred Int'l, Inc. High methionine derivatives of α-hordothionin for pathogen-control
US5850016A (en) 1996-03-20 1998-12-15 Pioneer Hi-Bred International, Inc. Alteration of amino acid compositions in seeds
US6072050A (en) 1996-06-11 2000-06-06 Pioneer Hi-Bred International, Inc. Synthetic promoters
CA2270289C (en) 1996-11-01 2005-09-27 Pioneer Hi-Bred International, Inc. Proteins with enhanced levels of essential amino acids
US5981840A (en) 1997-01-24 1999-11-09 Pioneer Hi-Bred International, Inc. Methods for agrobacterium-mediated transformation
AR014072A1 (es) 1998-02-26 2001-01-31 Pioneer Hi Bred Int Molecula de acido nucleico aislada que tiene una secuencia nucleotidica para un promotor que es capaz de iniciar una transcripcion constitutiva en unacelula de planta, construccion adn, vector, celula huesped, metodo para expresar en forma constitutiva una secuencia nucleotidica heteroloca en una
WO1999050427A2 (en) 1998-03-27 1999-10-07 Max-Plack-Gesellschaft Zur Förderung Der Wissenschaften E.V. Novel basal endosperm transfer cell layer (betl) specific genes
ATE309362T1 (de) 1998-08-20 2005-11-15 Pioneer Hi Bred Int Samen-bevorzugende promotoren
AU5788299A (en) 1998-08-28 2000-03-21 Pioneer Hi-Bred International, Inc. Seed-preferred promoters from (end) genes
WO2000028058A2 (en) 1998-11-09 2000-05-18 Pioneer Hi-Bred International, Inc. Transcriptional activator lec1 nucleic acids, polypeptides and their uses
US7531723B2 (en) 1999-04-16 2009-05-12 Pioneer Hi-Bred International, Inc. Modulation of cytokinin activity in plants
US7462481B2 (en) 2000-10-30 2008-12-09 Verdia, Inc. Glyphosate N-acetyltransferase (GAT) genes
AR039501A1 (es) 2002-04-30 2005-02-23 Verdia Inc Genes de glifosato n-acetil transferasa (gat)
EP1528104A1 (en) 2003-11-03 2005-05-04 Biogemma MEG1 endosperm-specific promoters and genes
US20090049569A1 (en) 2007-08-13 2009-02-19 Pioneer Hi-Bred International, Inc. Seed-Preferred Regulatory Elements
US7847160B2 (en) 2007-08-15 2010-12-07 Pioneer Hi-Bred International, Inc. Seed-preferred promoters
US7964770B2 (en) 2007-09-28 2011-06-21 Pioneer Hi-Bred International, Inc. Seed-preferred promoter from Sorghum kafirin gene
WO2009094704A1 (en) 2008-01-31 2009-08-06 The University Of Adelaide Seed specific expression in plants
WO2010019996A1 (en) 2008-08-18 2010-02-25 Australian Centre For Plant Functional Genomics Pty Ltd Seed active transcriptional control sequences
GB2465748B (en) 2008-11-25 2012-04-25 Algentech Sas Plant cell transformation method
GB2465749B (en) 2008-11-25 2013-05-08 Algentech Sas Plant cell transformation method
JP5208842B2 (ja) 2009-04-20 2013-06-12 株式会社カプコン ゲームシステム、ゲーム制御方法、プログラム、および、このプログラムを記録したコンピュータ読み取り可能な記録媒体
US20100281570A1 (en) 2009-05-04 2010-11-04 Pioneer Hi-Bred International, Inc. Maize 18kd oleosin seed-preferred regulatory element
US8466341B2 (en) 2009-05-04 2013-06-18 Pioneer Hi-Bred International, Inc. Maize 17KD oleosin seed-preferred regulatory element
AU2010246909B2 (en) 2009-05-13 2013-10-03 Basf Plant Science Company Gmbh Plant promoter operable in basal endosperm transfer layer of endosperm and uses thereof
MX2011013224A (es) 2009-06-09 2012-06-01 Pioneer Hi Bred Int Promotor de endospermo temprano y metodos de uso.
WO2011082310A2 (en) * 2009-12-30 2011-07-07 Pioneer Hi-Bred International, Inc. Methods and compositions for targeted polynucleotide modification
MX363013B (es) * 2011-02-25 2019-03-04 Recombinetics Inc Animales genéticamente modificados y métodos para su obtención.
AU2013266968B2 (en) 2012-05-25 2017-06-29 Emmanuelle CHARPENTIER Methods and compositions for RNA-directed target DNA modification and for RNA-directed modulation of transcription
US9267135B2 (en) 2013-06-04 2016-02-23 President And Fellows Of Harvard College RNA-guided transcriptional regulation
CA2930877A1 (en) 2013-11-18 2015-05-21 Crispr Therapeutics Ag Crispr-cas system materials and methods
CN103981215B (zh) * 2014-05-23 2016-06-29 安徽省农业科学院水稻研究所 一种用于基因工程的骨干质粒载体及应用
WO2015189693A1 (en) * 2014-06-12 2015-12-17 King Abdullah University Of Science And Technology Targeted viral-mediated plant genome editing using crispr/cas9
US9650617B2 (en) 2015-01-28 2017-05-16 Pioneer Hi-Bred International. Inc. CRISPR hybrid DNA/RNA polynucleotides and methods of use
EP3274453B1 (en) * 2015-03-26 2021-01-27 Editas Medicine, Inc. Crispr/cas-mediated gene conversion
US9790490B2 (en) 2015-06-18 2017-10-17 The Broad Institute Inc. CRISPR enzymes and systems
WO2017015015A1 (en) 2015-07-17 2017-01-26 Emory University Crispr-associated protein from francisella and uses related thereto
WO2018191715A2 (en) * 2017-04-14 2018-10-18 Synthetic Genomics, Inc. Polypeptides with type v crispr activity and uses thereof
EP3665279B1 (en) * 2017-08-09 2023-07-19 Benson Hill, Inc. Compositions and methods for modifying genomes
WO2019214604A1 (zh) * 2018-05-07 2019-11-14 中国农业大学 CRISPR/Cas效应蛋白及系统

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105142669A (zh) * 2012-12-06 2015-12-09 西格马-奥尔德里奇有限责任公司 基于crispr的基因组修饰和调控
CN109312316A (zh) * 2016-02-15 2019-02-05 本森希尔生物系统股份有限公司 修饰基因组的组合物和方法

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
RNA-activated DNA cleavage by the Type III-B CRISPR–Cas effector complex;Michael A. Estrella等;Genes & Dev.;第460-470页 *

Also Published As

Publication number Publication date
WO2019030695A1 (en) 2019-02-14
CN118497198A (zh) 2024-08-16
IL310452A (en) 2024-03-01
US20190048357A1 (en) 2019-02-14
AU2018315731A1 (en) 2020-03-19
US20190338296A1 (en) 2019-11-07
KR20250050118A (ko) 2025-04-14
JP2025185269A (ja) 2025-12-19
AU2024200896A1 (en) 2024-02-29
KR20200062184A (ko) 2020-06-03
IL272514A (en) 2020-03-31
KR102631985B1 (ko) 2024-02-01
IL272514B1 (en) 2025-06-01
JP7355730B2 (ja) 2023-10-03
EP3665279C0 (en) 2023-07-19
CN111373040A (zh) 2020-07-03
ES2960390T3 (es) 2024-03-04
BR112020002647A2 (pt) 2020-08-18
AU2018315731B2 (en) 2024-10-03
CA3072312C (en) 2026-01-06
IL272514B2 (en) 2025-10-01
CA3072312A1 (en) 2019-02-14
US20210024943A1 (en) 2021-01-28
US10837023B2 (en) 2020-11-17
JP2023169897A (ja) 2023-11-30
US20240392306A1 (en) 2024-11-28
US20230242925A1 (en) 2023-08-03
US20250304981A1 (en) 2025-10-02
EP4317443A2 (en) 2024-02-07
CA3284730A1 (en) 2026-03-02
JP2020533963A (ja) 2020-11-26
KR20240017981A (ko) 2024-02-08
US12600975B2 (en) 2026-04-14
EP4317443A3 (en) 2024-02-28
EP3665279A1 (en) 2020-06-17
US11624070B2 (en) 2023-04-11
EP4407034A3 (en) 2024-10-30
US10316324B2 (en) 2019-06-11
EP3665279B1 (en) 2023-07-19
US12146141B2 (en) 2024-11-19
EP4407034A2 (en) 2024-07-31
KR102789904B1 (ko) 2025-04-03

Similar Documents

Publication Publication Date Title
US12600975B2 (en) Compositions and methods for modifying genomes
US10113179B2 (en) Compositions and methods for modifying genomes
US20210180076A1 (en) Compositions and methods for genome editing in plants
US20250304982A1 (en) Compositions and methods for modifying genomes
BR122024020253A2 (pt) Composições e métodos para modificação de genomas

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant
TR01 Transfer of patent right

Effective date of registration: 20240827

Address after: Delaware, USA

Patentee after: Benson Hill Holdings Ltd.

Country or region after: U.S.A.

Address before: Missouri, USA

Patentee before: Benson Hill S.A.

Country or region before: U.S.A.

TR01 Transfer of patent right
TR01 Transfer of patent right

Effective date of registration: 20240912

Address after: American Texas

Patentee after: RICETEC, Inc.

Country or region after: U.S.A.

Address before: Delaware, USA

Patentee before: Benson Hill Holdings Ltd.

Country or region before: U.S.A.

TR01 Transfer of patent right