KR102747156B1 - 전립선 암의 검출 - Google Patents
전립선 암의 검출 Download PDFInfo
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- KR102747156B1 KR102747156B1 KR1020197025327A KR20197025327A KR102747156B1 KR 102747156 B1 KR102747156 B1 KR 102747156B1 KR 1020197025327 A KR1020197025327 A KR 1020197025327A KR 20197025327 A KR20197025327 A KR 20197025327A KR 102747156 B1 KR102747156 B1 KR 102747156B1
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2255198A4 (en) | 2008-02-15 | 2011-05-25 | Mayo Foundation | DETECTION OF NEOPLASM |
| US8361720B2 (en) | 2010-11-15 | 2013-01-29 | Exact Sciences Corporation | Real time cleavage assay |
| WO2015153284A1 (en) | 2014-03-31 | 2015-10-08 | Mayo Foundation For Medical Education And Research | Detecting colorectal neoplasm |
| US10184154B2 (en) | 2014-09-26 | 2019-01-22 | Mayo Foundation For Medical Education And Research | Detecting cholangiocarcinoma |
| WO2016160454A1 (en) | 2015-03-27 | 2016-10-06 | Exact Sciences Corporation | Detecting esophageal disorders |
| KR102892245B1 (ko) | 2017-01-27 | 2025-11-27 | 이그젝트 싸이언스 디블롭먼트 컴패니, 엘엘씨 | 메틸화된 dna 분석에 의한 결장 신조직형성의 검출 |
| WO2019108626A1 (en) | 2017-11-30 | 2019-06-06 | Mayo Foundation For Medical Education And Research | Detecting breast cancer |
| US10648025B2 (en) | 2017-12-13 | 2020-05-12 | Exact Sciences Development Company, Llc | Multiplex amplification detection assay II |
| CA3154354A1 (en) | 2019-10-31 | 2021-05-06 | William R. Taylor | Detecting ovarian cancer |
| CN113122634A (zh) * | 2020-01-14 | 2021-07-16 | 上海鹍远生物技术有限公司 | 检测dna甲基化的试剂及用途 |
| CN113528658B (zh) * | 2020-04-20 | 2024-09-13 | 江苏鹍远生物科技股份有限公司 | 甲状腺肿瘤良恶性鉴别模型及其应用 |
| AU2021268631A1 (en) * | 2020-05-04 | 2023-01-05 | Mayo Foundation For Medical Education And Research | Detecting pancreatic neuroendocrine tumors |
| WO2021237103A1 (en) | 2020-05-22 | 2021-11-25 | Wisconsin Alumni Research Foundation | Epigenetic modification of select genes as markers of hypersomnolence |
| CN112779334B (zh) * | 2021-02-01 | 2022-05-27 | 杭州医学院 | 一种用于前列腺癌早期筛查的甲基化标志物组合及筛选方法 |
| KR20230105973A (ko) * | 2022-01-05 | 2023-07-12 | 주식회사 젠큐릭스 | 특정 유전자의 CpG 메틸화 변화를 이용한 전립선암 진단용 조성물 및 이의 용도 |
| CN116287227B (zh) * | 2022-09-26 | 2024-11-05 | 武汉艾米森生命科技有限公司 | 用于诊断前列腺癌的试剂和试剂盒 |
| US20250270650A1 (en) * | 2023-12-12 | 2025-08-28 | Flagship Pioneering Innovations Vi, Llc | Methods for identifying genomic regions of low background methylation |
| CN118685521A (zh) * | 2024-08-26 | 2024-09-24 | 湖南宏雅基因技术有限公司 | 一种用于前列腺癌检测的引物探针组合物及其应用 |
| CN120005997B (zh) * | 2025-01-10 | 2025-09-09 | 中南大学湘雅三医院 | 前列腺癌特异性甲基化标志物的应用和检测试剂 |
Family Cites Families (118)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US1016854A (en) * | 1909-10-12 | 1912-02-06 | Nat Malleable Castings Co | Uncoupling mechanism for car-couplings. |
| US5288609A (en) | 1984-04-27 | 1994-02-22 | Enzo Diagnostics, Inc. | Capture sandwich hybridization method and composition |
| US4965188A (en) | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US5011769A (en) | 1985-12-05 | 1991-04-30 | Meiogenics U.S. Limited Partnership | Methods for detecting nucleic acid sequences |
| US5541308A (en) | 1986-11-24 | 1996-07-30 | Gen-Probe Incorporated | Nucleic acid probes for detection and/or quantitation of non-viral organisms |
| US5124246A (en) | 1987-10-15 | 1992-06-23 | Chiron Corporation | Nucleic acid multimers and amplified nucleic acid hybridization assays using same |
| US5359100A (en) | 1987-10-15 | 1994-10-25 | Chiron Corporation | Bifunctional blocked phosphoramidites useful in making nucleic acid mutimers |
| US5403711A (en) | 1987-11-30 | 1995-04-04 | University Of Iowa Research Foundation | Nucleic acid hybridization and amplification method for detection of specific sequences in which a complementary labeled nucleic acid probe is cleaved |
| CA1340807C (en) | 1988-02-24 | 1999-11-02 | Lawrence T. Malek | Nucleic acid amplification process |
| US5639611A (en) | 1988-12-12 | 1997-06-17 | City Of Hope | Allele specific polymerase chain reaction |
| US6800617B1 (en) | 1989-03-29 | 2004-10-05 | The Johns Hopkins University | Methods for restoring wild-type p53 gene function |
| US5527676A (en) | 1989-03-29 | 1996-06-18 | The Johns Hopkins University | Detection of loss of the wild-type P53 gene and kits therefor |
| US6677312B1 (en) | 1989-03-29 | 2004-01-13 | The Johns Hopkins University | Methods for restoring wild-type p53 gene function |
| US5362623A (en) | 1991-06-14 | 1994-11-08 | The John Hopkins University | Sequence specific DNA binding by p53 |
| CA2036946C (en) | 1990-04-06 | 2001-10-16 | Kenneth V. Deugau | Indexing linkers |
| US5494810A (en) | 1990-05-03 | 1996-02-27 | Cornell Research Foundation, Inc. | Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease |
| US5849481A (en) | 1990-07-27 | 1998-12-15 | Chiron Corporation | Nucleic acid hybridization assays employing large comb-type branched polynucleotides |
| US5352775A (en) | 1991-01-16 | 1994-10-04 | The Johns Hopkins Univ. | APC gene and nucleic acid probes derived therefrom |
| US6872816B1 (en) | 1996-01-24 | 2005-03-29 | Third Wave Technologies, Inc. | Nucleic acid detection kits |
| US5994069A (en) | 1996-01-24 | 1999-11-30 | Third Wave Technologies, Inc. | Detection of nucleic acids by multiple sequential invasive cleavages |
| US5846717A (en) | 1996-01-24 | 1998-12-08 | Third Wave Technologies, Inc. | Detection of nucleic acid sequences by invader-directed cleavage |
| US5338671A (en) | 1992-10-07 | 1994-08-16 | Eastman Kodak Company | DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody |
| SE501439C2 (sv) | 1993-06-22 | 1995-02-13 | Pharmacia Lkb Biotech | Sätt och anordning för analys av polynukleotidsekvenser |
| US6235470B1 (en) | 1993-11-12 | 2001-05-22 | The Johns Hopkins University School Of Medicine | Detection of neoplasia by analysis of saliva |
| AU8102694A (en) | 1993-11-17 | 1995-06-06 | Id Biomedical Corporation | Cycling probe cleavage detection of nucleic acid sequences |
| WO1995015373A2 (en) | 1993-11-30 | 1995-06-08 | Mcgill University | Inhibition of dna methyltransferase |
| WO1995029251A1 (en) | 1994-04-25 | 1995-11-02 | Applied Technology Genetics Corporation | Detection of mutation by resolvase cleavage |
| US5851770A (en) | 1994-04-25 | 1998-12-22 | Variagenics, Inc. | Detection of mismatches by resolvase cleavage using a magnetic bead support |
| WO1996020287A2 (en) | 1994-12-23 | 1996-07-04 | Behringwerke Aktiengesellschaft | Detection of nucleic acids by target-catalyzed product formation |
| US5882867A (en) | 1995-06-07 | 1999-03-16 | Dade Behring Marburg Gmbh | Detection of nucleic acids by formation of template-dependent product |
| US5773258A (en) | 1995-08-25 | 1998-06-30 | Roche Molecular Systems, Inc. | Nucleic acid amplification using a reversibly inactivated thermostable enzyme |
| US5854033A (en) | 1995-11-21 | 1998-12-29 | Yale University | Rolling circle replication reporter systems |
| US5965408A (en) | 1996-07-09 | 1999-10-12 | Diversa Corporation | Method of DNA reassembly by interrupting synthesis |
| JP2001517925A (ja) | 1995-12-22 | 2001-10-09 | ベーリングウエルケ、アクティエンゲゼルシャフト | 核酸の均質増幅および検出 |
| US5670325A (en) | 1996-08-14 | 1997-09-23 | Exact Laboratories, Inc. | Method for the detection of clonal populations of transformed cells in a genomically heterogeneous cellular sample |
| US5985557A (en) | 1996-01-24 | 1999-11-16 | Third Wave Technologies, Inc. | Invasive cleavage of nucleic acids |
| US5741650A (en) | 1996-01-30 | 1998-04-21 | Exact Laboratories, Inc. | Methods for detecting colon cancer from stool samples |
| US5891651A (en) | 1996-03-29 | 1999-04-06 | Mayo Foundation For Medical Education And Research | Methods of recovering colorectal epithelial cells or fragments thereof from stool |
| CA2252048C (en) | 1996-04-12 | 2008-03-11 | The Public Health Research Institute Of The City Of New York, Inc. | Detection probes, kits and assays |
| US6017704A (en) | 1996-06-03 | 2000-01-25 | The Johns Hopkins University School Of Medicine | Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids |
| US5786146A (en) | 1996-06-03 | 1998-07-28 | The Johns Hopkins University School Of Medicine | Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids |
| US5952178A (en) | 1996-08-14 | 1999-09-14 | Exact Laboratories | Methods for disease diagnosis from stool samples |
| US5928870A (en) | 1997-06-16 | 1999-07-27 | Exact Laboratories, Inc. | Methods for the detection of loss of heterozygosity |
| US6020137A (en) | 1996-08-14 | 2000-02-01 | Exact Laboratories, Inc. | Methods for the detection of loss of heterozygosity |
| US6096273A (en) | 1996-11-05 | 2000-08-01 | Clinical Micro Sensors | Electrodes linked via conductive oligomers to nucleic acids |
| US6251594B1 (en) | 1997-06-09 | 2001-06-26 | Usc/Norris Comprehensive Cancer Ctr. | Cancer diagnostic method based upon DNA methylation differences |
| US6013459A (en) | 1997-06-12 | 2000-01-11 | Clinical Micro Sensors, Inc. | Detection of analytes using reorganization energy |
| DE19754482A1 (de) | 1997-11-27 | 1999-07-01 | Epigenomics Gmbh | Verfahren zur Herstellung komplexer DNA-Methylierungs-Fingerabdrücke |
| US6063573A (en) | 1998-01-27 | 2000-05-16 | Clinical Micro Sensors, Inc. | Cycling probe technology using electron transfer detection |
| ATE224458T1 (de) | 1998-02-04 | 2002-10-15 | Variagenics Inc | Techniken zu detektion von fehlpaarungen |
| US6235502B1 (en) | 1998-09-18 | 2001-05-22 | Molecular Staging Inc. | Methods for selectively isolating DNA using rolling circle amplification |
| US7700324B1 (en) | 1998-11-03 | 2010-04-20 | The Johns Hopkins University School Of Medicine | Methylated CpG island amplification (MCA) |
| US6335193B1 (en) | 1999-04-15 | 2002-01-01 | Padmanabhan P Nair | Isolated colonocytes |
| ATE458831T1 (de) | 1999-12-07 | 2010-03-15 | Exact Sciences Corp | Verfahren zum nachweis von lungenneoplasmen in fäkalen proben |
| AU2001277521A1 (en) | 2000-06-30 | 2002-01-14 | Epigenomics Ag | Diagnosis of diseases associated with cell signalling |
| NZ524229A (en) | 2000-09-01 | 2006-01-27 | Epigenomics Ag | Method for simultaneous detection of many different methylation positions of specific cytosine residues in genomic DNA in the sequence context of 5'-CpG-3' |
| WO2003031932A2 (en) | 2001-10-05 | 2003-04-17 | Case Western Reserve University | Methods and compositions for detecting colon cancers |
| CN1650033A (zh) | 2002-03-07 | 2005-08-03 | 约翰霍普金斯大学医学院 | 针对与癌症相关的表观遗传学沉默基因的基因组筛选 |
| US7662594B2 (en) | 2002-09-20 | 2010-02-16 | New England Biolabs, Inc. | Helicase-dependent amplification of RNA |
| US20040146868A1 (en) | 2003-01-24 | 2004-07-29 | Epigenomics Ag | Methods and nucleic acids for the analysis of CpG dinucleotide methylation status associated with the development of peripheral zone prostate cancer |
| JP2007524369A (ja) | 2003-03-17 | 2007-08-30 | ザ ジョンズ ホプキンス ユニバーシティー | 膵癌における異常メチル化遺伝子 |
| EP2345745A1 (en) | 2003-06-23 | 2011-07-20 | Epigenomics AG | Methods and nucleic acids for analyses of colorectal cell proliferative disorders |
| CA2535910C (en) | 2003-08-14 | 2016-04-26 | Case Western Reserve University | Methods and compositions for detecting colon cancers |
| US8346482B2 (en) | 2003-08-22 | 2013-01-01 | Fernandez Dennis S | Integrated biosensor and simulation system for diagnosis and therapy |
| US7846693B2 (en) * | 2003-09-04 | 2010-12-07 | Human Genetic Signatures Pty. Ltd. | Nucleic acid detection assay |
| EP2354253A3 (en) | 2003-09-05 | 2011-11-16 | Trustees of Boston University | Method for non-invasive prenatal diagnosis |
| US20080039413A1 (en) | 2003-10-21 | 2008-02-14 | Morris David W | Novel compositions and methods in cancer |
| SI3296407T1 (sl) | 2004-03-02 | 2019-09-30 | The Johns Hopkins University | Mutacije gena PIK3CA pri rakih pri ljudeh |
| US20100273151A1 (en) * | 2004-05-28 | 2010-10-28 | Fred Hutchinson Cancer Research Center | Genome-wide analysis of palindrome formation and dna methylation |
| US8114587B2 (en) | 2005-06-23 | 2012-02-14 | Ambergen, Inc. | Methods for the detection of colorectal cancer |
| US7851154B2 (en) | 2005-07-22 | 2010-12-14 | Simon Daniel Spivack | GC tag-modified bisulfite genomic DNA sequencing for continuous methylation spectra |
| US20090191548A1 (en) * | 2005-09-29 | 2009-07-30 | Epigenomics Ag | Methods and nucleic acids for the analysis of gene expression associated with tissue classification |
| SI2385143T1 (sl) | 2006-02-02 | 2016-11-30 | The Board of Trustees of the Leland Stanford Junior University Office of the General Counsel | Neinvazivni genetski presejalni test ploda z digitalno analizo |
| JP4490382B2 (ja) | 2006-02-28 | 2010-06-23 | 富士フイルム株式会社 | 感熱転写受像シートおよびその製造方法 |
| WO2007116417A1 (en) | 2006-04-07 | 2007-10-18 | Silvia Sabbioni | Novel dna methylation markers useful for the diagnosis of neoplastic diseases |
| US20080081333A1 (en) | 2006-05-26 | 2008-04-03 | University Of Maryland, Baltimore | Methylated promoters as biomarkers of colon cancer |
| GB0700374D0 (en) | 2007-01-09 | 2007-02-14 | Oncomethylome Sciences S A | NDRG family methylation markers |
| US20080213870A1 (en) | 2007-03-01 | 2008-09-04 | Sean Wuxiong Cao | Methods for obtaining modified DNA from a biological specimen |
| CN101353695B (zh) | 2007-07-23 | 2013-05-01 | 上海市肿瘤研究所 | 尿沉淀dna甲基化谱式分析诊断膀胱癌的方法和试剂盒 |
| EP2255198A4 (en) | 2008-02-15 | 2011-05-25 | Mayo Foundation | DETECTION OF NEOPLASM |
| MX2010010161A (es) | 2008-03-15 | 2011-03-21 | Hologic Inc Star | Composiciones y metodos para el analisis de moleculas de acido nucleico durante reacciones de amplificacion. |
| WO2010062914A1 (en) | 2008-11-26 | 2010-06-03 | The Johns Hopkins University | Methods for identifying cancer risk |
| WO2010074924A1 (en) | 2008-12-23 | 2010-07-01 | University Of Utah Research Foundation | Identification and regulation of a novel dna demethylase system |
| EP2233590A1 (en) | 2009-01-28 | 2010-09-29 | AIT Austrian Institute of Technology GmbH | Methylation assay |
| US20120164238A1 (en) | 2009-02-03 | 2012-06-28 | Mdxhealth Sa | Methods of detecting colorectal cancer |
| CN102292458A (zh) | 2009-04-03 | 2011-12-21 | A&T株式会社 | 大肠肿瘤的检测方法 |
| WO2011037936A2 (en) * | 2009-09-24 | 2011-03-31 | Oregon Health & Science University | Detection of dna methylation of tal1, erg and/or cd40 to diagnose prostate cancer |
| US20120164110A1 (en) | 2009-10-14 | 2012-06-28 | Feinberg Andrew P | Differentially methylated regions of reprogrammed induced pluripotent stem cells, method and compositions thereof |
| US8709736B2 (en) | 2010-07-09 | 2014-04-29 | Medical Research Council | Biomarker for Barrett's Oesophagus |
| AU2011230633B2 (en) | 2010-03-26 | 2015-01-22 | Mayo Foundation For Medical Education And Research | Methods and materials for detecting colorectal neoplasm |
| WO2011126768A2 (en) | 2010-03-29 | 2011-10-13 | Mayo Foundation For Medical Education And Research | Methods and materials for detecting colorectal cancer and adenoma |
| EP2407555A1 (en) * | 2010-07-14 | 2012-01-18 | Fundació Institut de Recerca Hospital Universitari Vall d'Hebron, Fundació Privada | Methods and kits for the diagnosis of prostate cancer |
| US9957570B2 (en) | 2010-10-04 | 2018-05-01 | The Johns Hopkins University | DNA hypermethylation diagnostic biomarkers for colorectal cancer |
| US8916344B2 (en) | 2010-11-15 | 2014-12-23 | Exact Sciences Corporation | Methylation assay |
| US8715937B2 (en) | 2010-11-15 | 2014-05-06 | Exact Sciences Corporation | Mutation detection assay |
| US8361720B2 (en) | 2010-11-15 | 2013-01-29 | Exact Sciences Corporation | Real time cleavage assay |
| US20140113286A1 (en) | 2010-12-21 | 2014-04-24 | Sloan-Kettering Institute For Cancer Research | Epigenomic Markers of Cancer Metastasis |
| US20140057262A1 (en) | 2011-03-14 | 2014-02-27 | Mayo Foundation For Medical Education And Research | Methods and materials for using the contents of phagocytes to detect neoplasms |
| US9000146B2 (en) | 2011-05-12 | 2015-04-07 | Exact Sciences Corporation | Isolation of nucleic acids |
| US8808990B2 (en) | 2011-05-12 | 2014-08-19 | Exact Sciences Corporation | Serial isolation of multiple DNA targets from stool |
| US8993341B2 (en) | 2011-05-12 | 2015-03-31 | Exact Sciences Corporation | Removal of PCR inhibitors |
| US8980107B2 (en) | 2011-05-12 | 2015-03-17 | Exact Sciences Corporation | Spin filter |
| WO2012167145A2 (en) | 2011-06-01 | 2012-12-06 | University Of Southern California | Genome-scale analysis of aberrant dna methylation in colorectal cancer |
| US20130022974A1 (en) * | 2011-06-17 | 2013-01-24 | The Regents Of The University Of Michigan | Dna methylation profiles in cancer |
| WO2012175562A2 (en) | 2011-06-21 | 2012-12-27 | University Of Tartu | Methylation and microrna markers of early-stage non-small cell lung cancer |
| WO2013081188A1 (ja) | 2011-11-30 | 2013-06-06 | 独立行政法人国立がん研究センター | 誘導悪性幹細胞 |
| WO2013130880A1 (en) | 2012-02-28 | 2013-09-06 | The Johns Hopkins University | Hypermethylated gene markers for head and neck cancer |
| CA2865087A1 (en) | 2012-03-15 | 2013-09-19 | Mayo Foundation For Medical Education And Research | Methods and materials for noninvasive detection of colorectal neoplasia associated with inflammatory bowel disease |
| US10106854B2 (en) * | 2012-06-14 | 2018-10-23 | Aarhus Universitet | Biomarkers for prostate cancer |
| WO2014052855A1 (en) | 2012-09-27 | 2014-04-03 | Population Diagnostics, Inc. | Methods and compositions for screening and treating developmental disorders |
| EP2971179B1 (en) | 2013-03-14 | 2019-01-09 | Mayo Foundation for Medical Education and Research | Detecting neoplasm |
| JP6369857B2 (ja) | 2013-05-29 | 2018-08-08 | シスメックス株式会社 | 肝細胞癌に関する情報の取得方法、ならびに肝細胞癌に関する情報を取得するためのマーカーおよびキット |
| US9511050B2 (en) * | 2013-10-24 | 2016-12-06 | Georgetown University | Methods and compositions for treating cancer |
| WO2016049276A1 (en) | 2014-09-25 | 2016-03-31 | Moffitt Genetics Corporation | Prognostic tumor biomarkers |
| EP3034624A1 (en) | 2014-12-18 | 2016-06-22 | Hospital Clínic de Barcelona | Method for the prognosis of hepatocellular carcinoma |
| EP3037545A1 (en) * | 2014-12-23 | 2016-06-29 | The Provost, Fellows, Foundation Scholars, & the other members of Board, of the College of the Holy & Undiv. Trinity of Queen Elizabeth near Dublin | A DNA-methylation test for prostate cancer |
-
2018
- 2018-02-27 CA CA3054836A patent/CA3054836A1/en active Pending
- 2018-02-27 US US15/906,932 patent/US10934592B2/en active Active
- 2018-02-27 AU AU2018229294A patent/AU2018229294B2/en active Active
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- 2018-02-27 JP JP2019547146A patent/JP7356349B2/ja active Active
- 2018-02-27 CN CN202410376668.XA patent/CN118147307A/zh active Pending
- 2018-02-27 WO PCT/US2018/019982 patent/WO2018160576A1/en not_active Ceased
- 2018-02-27 CN CN201880014641.9A patent/CN110431241B/zh active Active
-
2021
- 2021-01-08 US US17/144,806 patent/US11697853B2/en active Active
-
2023
- 2023-05-24 US US18/323,031 patent/US20230416840A1/en active Pending
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-
2024
- 2024-09-13 AU AU2024219684A patent/AU2024219684A1/en active Pending
-
2025
- 2025-04-24 JP JP2025072146A patent/JP2025114618A/ja active Pending
Non-Patent Citations (2)
| Title |
|---|
| Biomark Med, 8(9): 1143-1150 (2014.11.17.)* |
| Curr Genomics, 12(7): 486-505 (2011.11.30.)* |
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