KR20250006325A - 전립선 암의 검출 - Google Patents
전립선 암의 검출 Download PDFInfo
- Publication number
- KR20250006325A KR20250006325A KR1020247042357A KR20247042357A KR20250006325A KR 20250006325 A KR20250006325 A KR 20250006325A KR 1020247042357 A KR1020247042357 A KR 1020247042357A KR 20247042357 A KR20247042357 A KR 20247042357A KR 20250006325 A KR20250006325 A KR 20250006325A
- Authority
- KR
- South Korea
- Prior art keywords
- methylation
- primer set
- sequence numbers
- set consisting
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 206010060862 Prostate cancer Diseases 0.000 title claims abstract description 149
- 208000000236 Prostatic Neoplasms Diseases 0.000 title claims abstract description 148
- 238000000034 method Methods 0.000 claims abstract description 250
- 239000000203 mixture Substances 0.000 claims abstract description 50
- 238000012216 screening Methods 0.000 claims abstract description 24
- 238000007069 methylation reaction Methods 0.000 claims description 416
- 230000011987 methylation Effects 0.000 claims description 414
- 108020004414 DNA Proteins 0.000 claims description 411
- 239000000523 sample Substances 0.000 claims description 304
- 150000007523 nucleic acids Chemical class 0.000 claims description 225
- 102000039446 nucleic acids Human genes 0.000 claims description 202
- 108020004707 nucleic acids Proteins 0.000 claims description 202
- 239000003550 marker Substances 0.000 claims description 144
- 125000003729 nucleotide group Chemical group 0.000 claims description 118
- LSNNMFCWUKXFEE-UHFFFAOYSA-M Bisulfite Chemical compound OS([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-M 0.000 claims description 106
- 239000002773 nucleotide Substances 0.000 claims description 104
- 108090000623 proteins and genes Proteins 0.000 claims description 104
- 238000003556 assay Methods 0.000 claims description 87
- 210000001519 tissue Anatomy 0.000 claims description 87
- 210000002307 prostate Anatomy 0.000 claims description 76
- 239000003153 chemical reaction reagent Substances 0.000 claims description 68
- 230000007067 DNA methylation Effects 0.000 claims description 65
- 238000003752 polymerase chain reaction Methods 0.000 claims description 59
- 108091029430 CpG site Proteins 0.000 claims description 56
- 230000003321 amplification Effects 0.000 claims description 54
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 54
- -1 AKR1B1_3644 Proteins 0.000 claims description 48
- 238000007855 methylation-specific PCR Methods 0.000 claims description 48
- 238000012360 testing method Methods 0.000 claims description 44
- 241000282414 Homo sapiens Species 0.000 claims description 43
- 108091034117 Oligonucleotide Proteins 0.000 claims description 43
- 239000012472 biological sample Substances 0.000 claims description 43
- 238000004458 analytical method Methods 0.000 claims description 38
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 33
- 238000012163 sequencing technique Methods 0.000 claims description 31
- 108091008146 restriction endonucleases Proteins 0.000 claims description 30
- 230000002759 chromosomal effect Effects 0.000 claims description 25
- 210000004369 blood Anatomy 0.000 claims description 23
- 239000008280 blood Substances 0.000 claims description 23
- 239000013068 control sample Substances 0.000 claims description 20
- 102100031487 Growth arrest-specific protein 6 Human genes 0.000 claims description 19
- 101000923005 Homo sapiens Growth arrest-specific protein 6 Proteins 0.000 claims description 19
- 238000003875 gradient-accelerated spectroscopy Methods 0.000 claims description 19
- 101000993981 Homo sapiens Inositol 1,4,5-trisphosphate receptor-interacting protein-like 1 Proteins 0.000 claims description 18
- 101000843449 Homo sapiens Transcription factor HES-5 Proteins 0.000 claims description 18
- 102100031529 Inositol 1,4,5-trisphosphate receptor-interacting protein-like 1 Human genes 0.000 claims description 18
- 108091006685 SLCO3A1 Proteins 0.000 claims description 18
- 102100022000 Solute carrier organic anion transporter family member 3A1 Human genes 0.000 claims description 18
- 102100030853 Transcription factor HES-5 Human genes 0.000 claims description 18
- 102100033764 Acyl-coenzyme A oxidase-like protein Human genes 0.000 claims description 17
- 102100034613 Annexin A2 Human genes 0.000 claims description 17
- 101000801174 Homo sapiens Acyl-coenzyme A oxidase-like protein Proteins 0.000 claims description 17
- 101000924474 Homo sapiens Annexin A2 Proteins 0.000 claims description 17
- 101001078431 Homo sapiens Hyaluronan and proteoglycan link protein 3 Proteins 0.000 claims description 17
- 101001049831 Homo sapiens Potassium channel subfamily K member 4 Proteins 0.000 claims description 17
- 101000712956 Homo sapiens Ras association domain-containing protein 2 Proteins 0.000 claims description 17
- 102100025260 Hyaluronan and proteoglycan link protein 3 Human genes 0.000 claims description 17
- 102100023205 Potassium channel subfamily K member 4 Human genes 0.000 claims description 17
- 102100033242 Ras association domain-containing protein 2 Human genes 0.000 claims description 17
- 102000016681 SLC4A Proteins Human genes 0.000 claims description 17
- 108091006267 SLC4A11 Proteins 0.000 claims description 17
- 108091026890 Coding region Proteins 0.000 claims description 12
- 230000001965 increasing effect Effects 0.000 claims description 12
- 238000005259 measurement Methods 0.000 claims description 11
- 230000001105 regulatory effect Effects 0.000 claims description 10
- 210000002700 urine Anatomy 0.000 claims description 10
- 239000013074 reference sample Substances 0.000 claims description 6
- 230000002441 reversible effect Effects 0.000 claims description 6
- 230000003247 decreasing effect Effects 0.000 claims description 5
- 238000004949 mass spectrometry Methods 0.000 claims description 5
- 101710163270 Nuclease Proteins 0.000 claims description 4
- 238000000926 separation method Methods 0.000 claims description 4
- 239000013610 patient sample Substances 0.000 claims description 2
- 108010076804 DNA Restriction Enzymes Proteins 0.000 claims 8
- 238000009585 enzyme analysis Methods 0.000 claims 8
- 238000012175 pyrosequencing Methods 0.000 claims 5
- 101000782147 Homo sapiens WD repeat-containing protein 20 Proteins 0.000 claims 1
- 230000001035 methylating effect Effects 0.000 claims 1
- 206010028980 Neoplasm Diseases 0.000 description 95
- 238000005516 engineering process Methods 0.000 description 62
- 239000013615 primer Substances 0.000 description 62
- 201000011510 cancer Diseases 0.000 description 61
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 48
- 238000011282 treatment Methods 0.000 description 41
- 238000006243 chemical reaction Methods 0.000 description 37
- 210000002381 plasma Anatomy 0.000 description 35
- 239000000090 biomarker Substances 0.000 description 32
- 239000000047 product Substances 0.000 description 31
- 210000004027 cell Anatomy 0.000 description 25
- 238000001514 detection method Methods 0.000 description 25
- 108091029523 CpG island Proteins 0.000 description 24
- 238000002474 experimental method Methods 0.000 description 24
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 23
- 201000010099 disease Diseases 0.000 description 22
- 230000035945 sensitivity Effects 0.000 description 22
- 230000008859 change Effects 0.000 description 21
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 description 20
- 229940104302 cytosine Drugs 0.000 description 20
- 239000000872 buffer Substances 0.000 description 19
- 230000008569 process Effects 0.000 description 18
- 102000004190 Enzymes Human genes 0.000 description 17
- 108090000790 Enzymes Proteins 0.000 description 17
- 239000012634 fragment Substances 0.000 description 16
- 238000003745 diagnosis Methods 0.000 description 15
- 108091033319 polynucleotide Proteins 0.000 description 15
- 102000040430 polynucleotide Human genes 0.000 description 15
- 239000002157 polynucleotide Substances 0.000 description 15
- 238000004393 prognosis Methods 0.000 description 15
- 208000024891 symptom Diseases 0.000 description 15
- 239000011324 bead Substances 0.000 description 14
- RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 description 14
- 239000000243 solution Substances 0.000 description 13
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 12
- LRSASMSXMSNRBT-UHFFFAOYSA-N 5-methylcytosine Chemical compound CC1=CNC(=O)N=C1N LRSASMSXMSNRBT-UHFFFAOYSA-N 0.000 description 12
- 238000003776 cleavage reaction Methods 0.000 description 12
- 230000000295 complement effect Effects 0.000 description 12
- 238000009396 hybridization Methods 0.000 description 12
- 230000001613 neoplastic effect Effects 0.000 description 12
- 238000011160 research Methods 0.000 description 12
- 238000010200 validation analysis Methods 0.000 description 12
- 241000283216 Phocidae Species 0.000 description 11
- 229920001184 polypeptide Polymers 0.000 description 11
- 108090000765 processed proteins & peptides Proteins 0.000 description 11
- 102000004196 processed proteins & peptides Human genes 0.000 description 11
- 230000007017 scission Effects 0.000 description 11
- 102000004169 proteins and genes Human genes 0.000 description 10
- 108700028369 Alleles Proteins 0.000 description 9
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 9
- 108020004999 messenger RNA Proteins 0.000 description 9
- 210000000056 organ Anatomy 0.000 description 9
- 229940035893 uracil Drugs 0.000 description 9
- 241001465754 Metazoa Species 0.000 description 8
- 108010006785 Taq Polymerase Proteins 0.000 description 8
- 230000001419 dependent effect Effects 0.000 description 8
- 230000014509 gene expression Effects 0.000 description 8
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 8
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 8
- 108091093088 Amplicon Proteins 0.000 description 7
- 102000007066 Prostate-Specific Antigen Human genes 0.000 description 7
- 108010072866 Prostate-Specific Antigen Proteins 0.000 description 7
- 230000000903 blocking effect Effects 0.000 description 7
- 208000035269 cancer or benign tumor Diseases 0.000 description 7
- 230000029087 digestion Effects 0.000 description 7
- 238000001976 enzyme digestion Methods 0.000 description 7
- 230000006870 function Effects 0.000 description 7
- 238000007403 mPCR Methods 0.000 description 7
- 239000000463 material Substances 0.000 description 7
- 230000002829 reductive effect Effects 0.000 description 7
- 229940113082 thymine Drugs 0.000 description 7
- 238000012546 transfer Methods 0.000 description 7
- 101150104383 ALOX5AP gene Proteins 0.000 description 6
- 102000007469 Actins Human genes 0.000 description 6
- 108010085238 Actins Proteins 0.000 description 6
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 6
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 6
- 108091092195 Intron Proteins 0.000 description 6
- 241000124008 Mammalia Species 0.000 description 6
- 101100236114 Mus musculus Lrrfip1 gene Proteins 0.000 description 6
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 6
- DWAQJAXMDSEUJJ-UHFFFAOYSA-M Sodium bisulfite Chemical group [Na+].OS([O-])=O DWAQJAXMDSEUJJ-UHFFFAOYSA-M 0.000 description 6
- 238000013459 approach Methods 0.000 description 6
- 238000010586 diagram Methods 0.000 description 6
- 238000010790 dilution Methods 0.000 description 6
- 239000012895 dilution Substances 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- 239000012530 fluid Substances 0.000 description 6
- 238000007477 logistic regression Methods 0.000 description 6
- 210000005267 prostate cell Anatomy 0.000 description 6
- 238000011002 quantification Methods 0.000 description 6
- 238000003753 real-time PCR Methods 0.000 description 6
- 235000010267 sodium hydrogen sulphite Nutrition 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- 238000003786 synthesis reaction Methods 0.000 description 6
- 239000002253 acid Substances 0.000 description 5
- 238000000137 annealing Methods 0.000 description 5
- 238000001369 bisulfite sequencing Methods 0.000 description 5
- 239000000356 contaminant Substances 0.000 description 5
- 239000003814 drug Substances 0.000 description 5
- 238000002866 fluorescence resonance energy transfer Methods 0.000 description 5
- 229940079826 hydrogen sulfite Drugs 0.000 description 5
- 238000000126 in silico method Methods 0.000 description 5
- 210000004185 liver Anatomy 0.000 description 5
- 238000002156 mixing Methods 0.000 description 5
- 239000012071 phase Substances 0.000 description 5
- 238000000746 purification Methods 0.000 description 5
- 238000000638 solvent extraction Methods 0.000 description 5
- 238000003860 storage Methods 0.000 description 5
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 4
- 229930024421 Adenine Natural products 0.000 description 4
- 241000282412 Homo Species 0.000 description 4
- 108060004795 Methyltransferase Proteins 0.000 description 4
- 102000044209 Tumor Suppressor Genes Human genes 0.000 description 4
- 108700025716 Tumor Suppressor Genes Proteins 0.000 description 4
- 150000007513 acids Chemical class 0.000 description 4
- 229960000643 adenine Drugs 0.000 description 4
- 230000027455 binding Effects 0.000 description 4
- 238000001574 biopsy Methods 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 4
- 210000001124 body fluid Anatomy 0.000 description 4
- 238000004925 denaturation Methods 0.000 description 4
- 230000036425 denaturation Effects 0.000 description 4
- 229940079593 drug Drugs 0.000 description 4
- 230000002255 enzymatic effect Effects 0.000 description 4
- 239000007850 fluorescent dye Substances 0.000 description 4
- 239000011888 foil Substances 0.000 description 4
- 238000002955 isolation Methods 0.000 description 4
- 230000004048 modification Effects 0.000 description 4
- 238000012986 modification Methods 0.000 description 4
- 238000007481 next generation sequencing Methods 0.000 description 4
- 230000003287 optical effect Effects 0.000 description 4
- 210000000496 pancreas Anatomy 0.000 description 4
- 239000002987 primer (paints) Substances 0.000 description 4
- 238000012545 processing Methods 0.000 description 4
- 239000000092 prognostic biomarker Substances 0.000 description 4
- 238000011472 radical prostatectomy Methods 0.000 description 4
- 239000011541 reaction mixture Substances 0.000 description 4
- 238000011084 recovery Methods 0.000 description 4
- 230000010076 replication Effects 0.000 description 4
- 210000002966 serum Anatomy 0.000 description 4
- 239000011534 wash buffer Substances 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 208000003200 Adenoma Diseases 0.000 description 3
- 206010001233 Adenoma benign Diseases 0.000 description 3
- 208000005623 Carcinogenesis Diseases 0.000 description 3
- 102000053602 DNA Human genes 0.000 description 3
- 108700039691 Genetic Promoter Regions Proteins 0.000 description 3
- 102000016397 Methyltransferase Human genes 0.000 description 3
- 238000012300 Sequence Analysis Methods 0.000 description 3
- 241000282887 Suidae Species 0.000 description 3
- 241000251539 Vertebrata <Metazoa> Species 0.000 description 3
- 102000044880 Wnt3A Human genes 0.000 description 3
- 108700013515 Wnt3A Proteins 0.000 description 3
- 230000001594 aberrant effect Effects 0.000 description 3
- 230000002411 adverse Effects 0.000 description 3
- 238000013103 analytical ultracentrifugation Methods 0.000 description 3
- 230000006399 behavior Effects 0.000 description 3
- 230000005540 biological transmission Effects 0.000 description 3
- 239000010839 body fluid Substances 0.000 description 3
- 230000036952 cancer formation Effects 0.000 description 3
- 229910052799 carbon Inorganic materials 0.000 description 3
- 231100000504 carcinogenesis Toxicity 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 210000001072 colon Anatomy 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 238000013461 design Methods 0.000 description 3
- 230000006326 desulfonation Effects 0.000 description 3
- 238000005869 desulfonation reaction Methods 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 239000000104 diagnostic biomarker Substances 0.000 description 3
- 238000002405 diagnostic procedure Methods 0.000 description 3
- WBZKQQHYRPRKNJ-UHFFFAOYSA-L disulfite Chemical compound [O-]S(=O)S([O-])(=O)=O WBZKQQHYRPRKNJ-UHFFFAOYSA-L 0.000 description 3
- 230000001973 epigenetic effect Effects 0.000 description 3
- 210000003238 esophagus Anatomy 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- 238000001917 fluorescence detection Methods 0.000 description 3
- 239000013315 hypercross-linked polymer Substances 0.000 description 3
- 238000000338 in vitro Methods 0.000 description 3
- 230000000977 initiatory effect Effects 0.000 description 3
- 230000003993 interaction Effects 0.000 description 3
- 210000000265 leukocyte Anatomy 0.000 description 3
- 239000003446 ligand Substances 0.000 description 3
- 239000011159 matrix material Substances 0.000 description 3
- 230000035772 mutation Effects 0.000 description 3
- 239000013642 negative control Substances 0.000 description 3
- 239000013612 plasmid Substances 0.000 description 3
- 239000013641 positive control Substances 0.000 description 3
- 238000001556 precipitation Methods 0.000 description 3
- QHGVXILFMXYDRS-UHFFFAOYSA-N pyraclofos Chemical compound C1=C(OP(=O)(OCC)SCCC)C=NN1C1=CC=C(Cl)C=C1 QHGVXILFMXYDRS-UHFFFAOYSA-N 0.000 description 3
- 125000000714 pyrimidinyl group Chemical group 0.000 description 3
- 230000028327 secretion Effects 0.000 description 3
- 239000000377 silicon dioxide Substances 0.000 description 3
- 210000002784 stomach Anatomy 0.000 description 3
- 238000000108 ultra-filtration Methods 0.000 description 3
- 238000012795 verification Methods 0.000 description 3
- 101150068520 wnt3a gene Proteins 0.000 description 3
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 2
- 206010003445 Ascites Diseases 0.000 description 2
- 241000972773 Aulopiformes Species 0.000 description 2
- 206010009944 Colon cancer Diseases 0.000 description 2
- 208000001333 Colorectal Neoplasms Diseases 0.000 description 2
- 108020004635 Complementary DNA Proteins 0.000 description 2
- 230000030933 DNA methylation on cytosine Effects 0.000 description 2
- 230000009946 DNA mutation Effects 0.000 description 2
- LCGLNKUTAGEVQW-UHFFFAOYSA-N Dimethyl ether Chemical compound COC LCGLNKUTAGEVQW-UHFFFAOYSA-N 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 102100031780 Endonuclease Human genes 0.000 description 2
- 108010042407 Endonucleases Proteins 0.000 description 2
- 102000003960 Ligases Human genes 0.000 description 2
- 108090000364 Ligases Proteins 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- 206010054949 Metaplasia Diseases 0.000 description 2
- 238000012408 PCR amplification Methods 0.000 description 2
- 108091028664 Ribonucleotide Proteins 0.000 description 2
- 241000282849 Ruminantia Species 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 241000282898 Sus scrofa Species 0.000 description 2
- 241000700605 Viruses Species 0.000 description 2
- 230000002159 abnormal effect Effects 0.000 description 2
- 239000000443 aerosol Substances 0.000 description 2
- 229910052782 aluminium Inorganic materials 0.000 description 2
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 2
- 239000012491 analyte Substances 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 210000003445 biliary tract Anatomy 0.000 description 2
- 210000000349 chromosome Anatomy 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 230000008878 coupling Effects 0.000 description 2
- 238000010168 coupling process Methods 0.000 description 2
- 238000005859 coupling reaction Methods 0.000 description 2
- 230000001186 cumulative effect Effects 0.000 description 2
- 239000005547 deoxyribonucleotide Substances 0.000 description 2
- 125000002637 deoxyribonucleotide group Chemical group 0.000 description 2
- 230000009977 dual effect Effects 0.000 description 2
- 239000003623 enhancer Substances 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 235000019688 fish Nutrition 0.000 description 2
- LNTHITQWFMADLM-UHFFFAOYSA-N gallic acid Chemical compound OC(=O)C1=CC(O)=C(O)C(O)=C1 LNTHITQWFMADLM-UHFFFAOYSA-N 0.000 description 2
- 230000002496 gastric effect Effects 0.000 description 2
- 238000001502 gel electrophoresis Methods 0.000 description 2
- 230000002068 genetic effect Effects 0.000 description 2
- 238000012268 genome sequencing Methods 0.000 description 2
- 238000003384 imaging method Methods 0.000 description 2
- 230000001976 improved effect Effects 0.000 description 2
- 230000001939 inductive effect Effects 0.000 description 2
- 238000007834 ligase chain reaction Methods 0.000 description 2
- 230000000670 limiting effect Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000013507 mapping Methods 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 239000000155 melt Substances 0.000 description 2
- 230000015689 metaplastic ossification Effects 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 238000007857 nested PCR Methods 0.000 description 2
- 108091027963 non-coding RNA Proteins 0.000 description 2
- 102000042567 non-coding RNA Human genes 0.000 description 2
- 230000001855 preneoplastic effect Effects 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 210000000064 prostate epithelial cell Anatomy 0.000 description 2
- 238000004445 quantitative analysis Methods 0.000 description 2
- 238000012113 quantitative test Methods 0.000 description 2
- 238000013102 re-test Methods 0.000 description 2
- 230000001373 regressive effect Effects 0.000 description 2
- 230000000717 retained effect Effects 0.000 description 2
- 239000002336 ribonucleotide Substances 0.000 description 2
- 125000002652 ribonucleotide group Chemical group 0.000 description 2
- 235000019515 salmon Nutrition 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- 238000010561 standard procedure Methods 0.000 description 2
- 238000007619 statistical method Methods 0.000 description 2
- 238000006277 sulfonation reaction Methods 0.000 description 2
- 238000001356 surgical procedure Methods 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 230000002123 temporal effect Effects 0.000 description 2
- 230000001225 therapeutic effect Effects 0.000 description 2
- 238000013518 transcription Methods 0.000 description 2
- 230000035897 transcription Effects 0.000 description 2
- RYHBNJHYFVUHQT-UHFFFAOYSA-N 1,4-Dioxane Chemical compound C1COCCO1 RYHBNJHYFVUHQT-UHFFFAOYSA-N 0.000 description 1
- HWPZZUQOWRWFDB-UHFFFAOYSA-N 1-methylcytosine Chemical compound CN1C=CC(N)=NC1=O HWPZZUQOWRWFDB-UHFFFAOYSA-N 0.000 description 1
- 108020005065 3' Flanking Region Proteins 0.000 description 1
- JYCQQPHGFMYQCF-UHFFFAOYSA-N 4-tert-Octylphenol monoethoxylate Chemical compound CC(C)(C)CC(C)(C)C1=CC=C(OCCO)C=C1 JYCQQPHGFMYQCF-UHFFFAOYSA-N 0.000 description 1
- 108020005029 5' Flanking Region Proteins 0.000 description 1
- VHTUHGNVVZPWGO-UHFFFAOYSA-N 7-(2-hydroxyethyl)-1,3-dimethyl-8-(pyridin-3-ylmethyl)purine-2,6-dione Chemical compound OCCN1C=2C(=O)N(C)C(=O)N(C)C=2N=C1CC1=CC=CN=C1 VHTUHGNVVZPWGO-UHFFFAOYSA-N 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
- 102100027265 Aldo-keto reductase family 1 member B1 Human genes 0.000 description 1
- 102100024504 Bone morphogenetic protein 3 Human genes 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 241000282832 Camelidae Species 0.000 description 1
- 241000282472 Canis lupus familiaris Species 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- 241001466804 Carnivora Species 0.000 description 1
- 241000282994 Cervidae Species 0.000 description 1
- 108091006146 Channels Proteins 0.000 description 1
- 241001227713 Chiron Species 0.000 description 1
- 108091033380 Coding strand Proteins 0.000 description 1
- 241000777300 Congiopodidae Species 0.000 description 1
- 102000012410 DNA Ligases Human genes 0.000 description 1
- 108010061982 DNA Ligases Proteins 0.000 description 1
- 230000004544 DNA amplification Effects 0.000 description 1
- 238000007400 DNA extraction Methods 0.000 description 1
- 238000000018 DNA microarray Methods 0.000 description 1
- 239000003155 DNA primer Substances 0.000 description 1
- 230000004543 DNA replication Effects 0.000 description 1
- 238000001712 DNA sequencing Methods 0.000 description 1
- 108700029231 Developmental Genes Proteins 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- 108010067770 Endopeptidase K Proteins 0.000 description 1
- 208000010228 Erectile Dysfunction Diseases 0.000 description 1
- 108700039887 Essential Genes Proteins 0.000 description 1
- 241000206602 Eukaryota Species 0.000 description 1
- 241000282326 Felis catus Species 0.000 description 1
- 102100040680 Formin-binding protein 1 Human genes 0.000 description 1
- 102100037388 Gasdermin-D Human genes 0.000 description 1
- 241000282818 Giraffidae Species 0.000 description 1
- 101000836540 Homo sapiens Aldo-keto reductase family 1 member B1 Proteins 0.000 description 1
- 101000762375 Homo sapiens Bone morphogenetic protein 3 Proteins 0.000 description 1
- 101000847538 Homo sapiens Flotillin-1 Proteins 0.000 description 1
- 101000892722 Homo sapiens Formin-binding protein 1 Proteins 0.000 description 1
- 101001026262 Homo sapiens Gasdermin-D Proteins 0.000 description 1
- 101001033889 Homo sapiens Inositol 1,4,5-trisphosphate receptor-interacting protein-like 2 Proteins 0.000 description 1
- 101001048969 Homo sapiens Protein FAM78A Proteins 0.000 description 1
- 101000995300 Homo sapiens Protein NDRG2 Proteins 0.000 description 1
- 101000864786 Homo sapiens Secreted frizzled-related protein 2 Proteins 0.000 description 1
- 101000830781 Homo sapiens Tropomyosin alpha-4 chain Proteins 0.000 description 1
- 101000785609 Homo sapiens Zinc finger protein 655 Proteins 0.000 description 1
- 102100039741 Inositol 1,4,5-trisphosphate receptor-interacting protein-like 2 Human genes 0.000 description 1
- 101800001509 Large capsid protein Proteins 0.000 description 1
- 229920000106 Liquid crystal polymer Polymers 0.000 description 1
- 239000007993 MOPS buffer Substances 0.000 description 1
- 206010064912 Malignant transformation Diseases 0.000 description 1
- 241001529936 Murinae Species 0.000 description 1
- 108020003217 Nuclear RNA Proteins 0.000 description 1
- 102000043141 Nuclear RNA Human genes 0.000 description 1
- 108020005187 Oligonucleotide Probes Proteins 0.000 description 1
- 108700026244 Open Reading Frames Proteins 0.000 description 1
- 206010061902 Pancreatic neoplasm Diseases 0.000 description 1
- 241001278385 Panthera tigris altaica Species 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 108010002747 Pfu DNA polymerase Proteins 0.000 description 1
- 241000283150 Phoca vitulina Species 0.000 description 1
- 240000007643 Phytolacca americana Species 0.000 description 1
- ZYFVNVRFVHJEIU-UHFFFAOYSA-N PicoGreen Chemical compound CN(C)CCCN(CCCN(C)C)C1=CC(=CC2=[N+](C3=CC=CC=C3S2)C)C2=CC=CC=C2N1C1=CC=CC=C1 ZYFVNVRFVHJEIU-UHFFFAOYSA-N 0.000 description 1
- 229920001213 Polysorbate 20 Polymers 0.000 description 1
- 241000288906 Primates Species 0.000 description 1
- 102100023831 Protein FAM78A Human genes 0.000 description 1
- 102100034436 Protein NDRG2 Human genes 0.000 description 1
- 108010066717 Q beta Replicase Proteins 0.000 description 1
- 238000011529 RT qPCR Methods 0.000 description 1
- 101100209986 Rattus norvegicus Slc18a1 gene Proteins 0.000 description 1
- 102100029753 Reduced folate transporter Human genes 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 241000566107 Scolopax Species 0.000 description 1
- 102100030054 Secreted frizzled-related protein 2 Human genes 0.000 description 1
- 108091081021 Sense strand Proteins 0.000 description 1
- 108020004682 Single-Stranded DNA Proteins 0.000 description 1
- 238000002105 Southern blotting Methods 0.000 description 1
- LSNNMFCWUKXFEE-UHFFFAOYSA-N Sulfurous acid Chemical group OS(O)=O LSNNMFCWUKXFEE-UHFFFAOYSA-N 0.000 description 1
- 101710137500 T7 RNA polymerase Proteins 0.000 description 1
- 102100024944 Tropomyosin alpha-4 chain Human genes 0.000 description 1
- 206010046543 Urinary incontinence Diseases 0.000 description 1
- 101150019524 WNT2 gene Proteins 0.000 description 1
- 102000052556 Wnt-2 Human genes 0.000 description 1
- 108700020986 Wnt-2 Proteins 0.000 description 1
- 102100026494 Zinc finger protein 655 Human genes 0.000 description 1
- 238000005904 alkaline hydrolysis reaction Methods 0.000 description 1
- 238000007844 allele-specific PCR Methods 0.000 description 1
- 125000003275 alpha amino acid group Chemical group 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 210000003484 anatomy Anatomy 0.000 description 1
- 210000004102 animal cell Anatomy 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 230000000692 anti-sense effect Effects 0.000 description 1
- 210000000436 anus Anatomy 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 238000010420 art technique Methods 0.000 description 1
- 238000007845 assembly PCR Methods 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000013060 biological fluid Substances 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 210000000601 blood cell Anatomy 0.000 description 1
- 244000309464 bull Species 0.000 description 1
- 238000004422 calculation algorithm Methods 0.000 description 1
- 125000004432 carbon atom Chemical group C* 0.000 description 1
- 210000004534 cecum Anatomy 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 239000007806 chemical reaction intermediate Substances 0.000 description 1
- VZWXIQHBIQLMPN-UHFFFAOYSA-N chromane Chemical class C1=CC=C2CCCOC2=C1 VZWXIQHBIQLMPN-UHFFFAOYSA-N 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 230000000112 colonic effect Effects 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 239000002299 complementary DNA Substances 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 238000009109 curative therapy Methods 0.000 description 1
- 238000013211 curve analysis Methods 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 230000001351 cycling effect Effects 0.000 description 1
- CTMZLDSMFCVUNX-VMIOUTBZSA-N cytidylyl-(3'->5')-guanosine Chemical compound O=C1N=C(N)C=CN1[C@H]1[C@H](O)[C@H](OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=C(C(N=C(N)N3)=O)N=C2)O)[C@@H](CO)O1 CTMZLDSMFCVUNX-VMIOUTBZSA-N 0.000 description 1
- SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 description 1
- SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 description 1
- RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 description 1
- HAAZLUGHYHWQIW-KVQBGUIXSA-N dGTP Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HAAZLUGHYHWQIW-KVQBGUIXSA-N 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000009615 deamination Effects 0.000 description 1
- 238000006481 deamination reaction Methods 0.000 description 1
- 230000034994 death Effects 0.000 description 1
- 231100000517 death Toxicity 0.000 description 1
- 238000003066 decision tree Methods 0.000 description 1
- 239000013578 denaturing buffer Substances 0.000 description 1
- 238000003795 desorption Methods 0.000 description 1
- 239000003599 detergent Substances 0.000 description 1
- 238000000502 dialysis Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 238000007847 digital PCR Methods 0.000 description 1
- SBZXBUIDTXKZTM-UHFFFAOYSA-N diglyme Chemical compound COCCOCCOC SBZXBUIDTXKZTM-UHFFFAOYSA-N 0.000 description 1
- 239000012470 diluted sample Substances 0.000 description 1
- 150000002012 dioxanes Chemical class 0.000 description 1
- 208000035475 disorder Diseases 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000002651 drug therapy Methods 0.000 description 1
- 210000001198 duodenum Anatomy 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 238000002330 electrospray ionisation mass spectrometry Methods 0.000 description 1
- 239000012149 elution buffer Substances 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 238000001839 endoscopy Methods 0.000 description 1
- 239000005447 environmental material Substances 0.000 description 1
- 230000006862 enzymatic digestion Effects 0.000 description 1
- LYCAIKOWRPUZTN-UHFFFAOYSA-N ethylene glycol Natural products OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000007672 fourth generation sequencing Methods 0.000 description 1
- 230000037433 frameshift Effects 0.000 description 1
- 210000000232 gallbladder Anatomy 0.000 description 1
- 229940074391 gallic acid Drugs 0.000 description 1
- 235000004515 gallic acid Nutrition 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 230000007274 generation of a signal involved in cell-cell signaling Effects 0.000 description 1
- 230000000762 glandular Effects 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 239000008241 heterogeneous mixture Substances 0.000 description 1
- 230000001744 histochemical effect Effects 0.000 description 1
- 238000007849 hot-start PCR Methods 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- WGCNASOHLSPBMP-UHFFFAOYSA-N hydroxyacetaldehyde Natural products OCC=O WGCNASOHLSPBMP-UHFFFAOYSA-N 0.000 description 1
- 230000006607 hypermethylation Effects 0.000 description 1
- 201000001881 impotence Diseases 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000000543 intermediate Substances 0.000 description 1
- 238000007851 intersequence-specific PCR Methods 0.000 description 1
- 238000007852 inverse PCR Methods 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 108010026228 mRNA guanylyltransferase Proteins 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 230000036212 malign transformation Effects 0.000 description 1
- 230000036210 malignancy Effects 0.000 description 1
- 208000015486 malignant pancreatic neoplasm Diseases 0.000 description 1
- 238000007726 management method Methods 0.000 description 1
- 238000012067 mathematical method Methods 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 238000007856 miniprimer PCR Methods 0.000 description 1
- 239000003068 molecular probe Substances 0.000 description 1
- 210000003097 mucus Anatomy 0.000 description 1
- 238000007838 multiplex ligation-dependent probe amplification Methods 0.000 description 1
- 239000000101 novel biomarker Substances 0.000 description 1
- 210000004940 nucleus Anatomy 0.000 description 1
- 229940124276 oligodeoxyribonucleotide Drugs 0.000 description 1
- 239000002751 oligonucleotide probe Substances 0.000 description 1
- 239000013307 optical fiber Substances 0.000 description 1
- 201000002528 pancreatic cancer Diseases 0.000 description 1
- 208000008443 pancreatic carcinoma Diseases 0.000 description 1
- 230000036961 partial effect Effects 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 238000003909 pattern recognition Methods 0.000 description 1
- 210000004303 peritoneum Anatomy 0.000 description 1
- 230000008488 polyadenylation Effects 0.000 description 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
- 230000001124 posttranscriptional effect Effects 0.000 description 1
- 235000008476 powdered milk Nutrition 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000004886 process control Methods 0.000 description 1
- 230000002062 proliferating effect Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 238000012207 quantitative assay Methods 0.000 description 1
- 239000002516 radical scavenger Substances 0.000 description 1
- 230000002285 radioactive effect Effects 0.000 description 1
- 239000011535 reaction buffer Substances 0.000 description 1
- 210000000664 rectum Anatomy 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 230000003252 repetitive effect Effects 0.000 description 1
- 238000010839 reverse transcription Methods 0.000 description 1
- 238000003757 reverse transcription PCR Methods 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 229920002477 rna polymer Polymers 0.000 description 1
- 238000005096 rolling process Methods 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 238000005185 salting out Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 230000019491 signal transduction Effects 0.000 description 1
- 210000000813 small intestine Anatomy 0.000 description 1
- WBHQBSYUUJJSRZ-UHFFFAOYSA-M sodium bisulfate Chemical compound [Na+].OS([O-])(=O)=O WBHQBSYUUJJSRZ-UHFFFAOYSA-M 0.000 description 1
- 229910000342 sodium bisulfate Inorganic materials 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 230000009870 specific binding Effects 0.000 description 1
- 238000011895 specific detection Methods 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 230000002269 spontaneous effect Effects 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000008093 supporting effect Effects 0.000 description 1
- RLNWRDKVJSXXPP-UHFFFAOYSA-N tert-butyl 2-[(2-bromoanilino)methyl]piperidine-1-carboxylate Chemical compound CC(C)(C)OC(=O)N1CCCCC1CNC1=CC=CC=C1Br RLNWRDKVJSXXPP-UHFFFAOYSA-N 0.000 description 1
- 238000005382 thermal cycling Methods 0.000 description 1
- 238000007862 touchdown PCR Methods 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 230000032258 transport Effects 0.000 description 1
- 239000000107 tumor biomarker Substances 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
- 229960005486 vaccine Drugs 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
- C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/154—Methylation markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/16—Primer sets for multiplex assays
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Genetics & Genomics (AREA)
- Pathology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Hospice & Palliative Care (AREA)
- Oncology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201762464800P | 2017-02-28 | 2017-02-28 | |
| US62/464,800 | 2017-02-28 | ||
| KR1020197025327A KR102747156B1 (ko) | 2017-02-28 | 2018-02-27 | 전립선 암의 검출 |
| PCT/US2018/019982 WO2018160576A1 (en) | 2017-02-28 | 2018-02-27 | Detecting prostate cancer |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020197025327A Division KR102747156B1 (ko) | 2017-02-28 | 2018-02-27 | 전립선 암의 검출 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| KR20250006325A true KR20250006325A (ko) | 2025-01-10 |
Family
ID=63245681
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020197025327A Active KR102747156B1 (ko) | 2017-02-28 | 2018-02-27 | 전립선 암의 검출 |
| KR1020247042357A Pending KR20250006325A (ko) | 2017-02-28 | 2018-02-27 | 전립선 암의 검출 |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020197025327A Active KR102747156B1 (ko) | 2017-02-28 | 2018-02-27 | 전립선 암의 검출 |
Country Status (8)
| Country | Link |
|---|---|
| US (3) | US10934592B2 (enExample) |
| EP (1) | EP3589732A4 (enExample) |
| JP (3) | JP7356349B2 (enExample) |
| KR (2) | KR102747156B1 (enExample) |
| CN (2) | CN118147307A (enExample) |
| AU (2) | AU2018229294B2 (enExample) |
| CA (1) | CA3054836A1 (enExample) |
| WO (1) | WO2018160576A1 (enExample) |
Families Citing this family (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2522542T3 (es) | 2008-02-15 | 2014-11-17 | Mayo Foundation For Medical Education And Research | Detección de neoplasia a partir de una muestra de heces |
| US8361720B2 (en) | 2010-11-15 | 2013-01-29 | Exact Sciences Corporation | Real time cleavage assay |
| US10301680B2 (en) | 2014-03-31 | 2019-05-28 | Mayo Foundation For Medical Education And Research | Detecting colorectal neoplasm |
| US10184154B2 (en) | 2014-09-26 | 2019-01-22 | Mayo Foundation For Medical Education And Research | Detecting cholangiocarcinoma |
| US10435755B2 (en) | 2015-03-27 | 2019-10-08 | Exact Sciences Development Company, Llc | Detecting esophageal disorders |
| WO2018140781A1 (en) | 2017-01-27 | 2018-08-02 | Exact Sciences Development Company, Llc | Detection of colon neoplasia by analysis of methylated dna |
| US10975443B2 (en) | 2017-11-30 | 2021-04-13 | Mayo Foundation For Medical Education And Research | Detecting breast cancer |
| US10648025B2 (en) | 2017-12-13 | 2020-05-12 | Exact Sciences Development Company, Llc | Multiplex amplification detection assay II |
| JP2022553575A (ja) | 2019-10-31 | 2022-12-23 | マヨ ファウンデーション フォア メディカル エデュケーション アンド リサーチ | 卵巣癌の検出 |
| CN113122635A (zh) * | 2020-01-14 | 2021-07-16 | 上海鹍远生物技术有限公司 | 检测dna甲基化的试剂及用途 |
| CN113528658B (zh) * | 2020-04-20 | 2024-09-13 | 江苏鹍远生物科技股份有限公司 | 甲状腺肿瘤良恶性鉴别模型及其应用 |
| CN115551880A (zh) * | 2020-05-04 | 2022-12-30 | 梅约医学教育与研究基金会 | 检测胰腺神经内分泌肿瘤 |
| EP4467663A3 (en) | 2020-05-22 | 2025-02-26 | Wisconsin Alumni Research Foundation | Epigenetic modification of select genes as markers of hypersomnolence |
| CN112779334B (zh) * | 2021-02-01 | 2022-05-27 | 杭州医学院 | 一种用于前列腺癌早期筛查的甲基化标志物组合及筛选方法 |
| KR20230105973A (ko) * | 2022-01-05 | 2023-07-12 | 주식회사 젠큐릭스 | 특정 유전자의 CpG 메틸화 변화를 이용한 전립선암 진단용 조성물 및 이의 용도 |
| CN116287227B (zh) * | 2022-09-26 | 2024-11-05 | 武汉艾米森生命科技有限公司 | 用于诊断前列腺癌的试剂和试剂盒 |
| WO2025128691A1 (en) * | 2023-12-12 | 2025-06-19 | Flagship Pioneering Innovations Vi, Llc | Methods for identifying genomic regions of low background methylation |
| CN118685521A (zh) * | 2024-08-26 | 2024-09-24 | 湖南宏雅基因技术有限公司 | 一种用于前列腺癌检测的引物探针组合物及其应用 |
| CN120005997B (zh) * | 2025-01-10 | 2025-09-09 | 中南大学湘雅三医院 | 前列腺癌特异性甲基化标志物的应用和检测试剂 |
Family Cites Families (118)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US1016854A (en) * | 1909-10-12 | 1912-02-06 | Nat Malleable Castings Co | Uncoupling mechanism for car-couplings. |
| US5288609A (en) | 1984-04-27 | 1994-02-22 | Enzo Diagnostics, Inc. | Capture sandwich hybridization method and composition |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4965188A (en) | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
| US5011769A (en) | 1985-12-05 | 1991-04-30 | Meiogenics U.S. Limited Partnership | Methods for detecting nucleic acid sequences |
| US5541308A (en) | 1986-11-24 | 1996-07-30 | Gen-Probe Incorporated | Nucleic acid probes for detection and/or quantitation of non-viral organisms |
| US5359100A (en) | 1987-10-15 | 1994-10-25 | Chiron Corporation | Bifunctional blocked phosphoramidites useful in making nucleic acid mutimers |
| US5124246A (en) | 1987-10-15 | 1992-06-23 | Chiron Corporation | Nucleic acid multimers and amplified nucleic acid hybridization assays using same |
| US5403711A (en) | 1987-11-30 | 1995-04-04 | University Of Iowa Research Foundation | Nucleic acid hybridization and amplification method for detection of specific sequences in which a complementary labeled nucleic acid probe is cleaved |
| CA1340807C (en) | 1988-02-24 | 1999-11-02 | Lawrence T. Malek | Nucleic acid amplification process |
| US5639611A (en) | 1988-12-12 | 1997-06-17 | City Of Hope | Allele specific polymerase chain reaction |
| US6677312B1 (en) | 1989-03-29 | 2004-01-13 | The Johns Hopkins University | Methods for restoring wild-type p53 gene function |
| US5527676A (en) | 1989-03-29 | 1996-06-18 | The Johns Hopkins University | Detection of loss of the wild-type P53 gene and kits therefor |
| US5362623A (en) | 1991-06-14 | 1994-11-08 | The John Hopkins University | Sequence specific DNA binding by p53 |
| US6800617B1 (en) | 1989-03-29 | 2004-10-05 | The Johns Hopkins University | Methods for restoring wild-type p53 gene function |
| CA2036946C (en) | 1990-04-06 | 2001-10-16 | Kenneth V. Deugau | Indexing linkers |
| US5494810A (en) | 1990-05-03 | 1996-02-27 | Cornell Research Foundation, Inc. | Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease |
| US5849481A (en) | 1990-07-27 | 1998-12-15 | Chiron Corporation | Nucleic acid hybridization assays employing large comb-type branched polynucleotides |
| US5352775A (en) | 1991-01-16 | 1994-10-04 | The Johns Hopkins Univ. | APC gene and nucleic acid probes derived therefrom |
| US5994069A (en) | 1996-01-24 | 1999-11-30 | Third Wave Technologies, Inc. | Detection of nucleic acids by multiple sequential invasive cleavages |
| US5846717A (en) | 1996-01-24 | 1998-12-08 | Third Wave Technologies, Inc. | Detection of nucleic acid sequences by invader-directed cleavage |
| US6872816B1 (en) | 1996-01-24 | 2005-03-29 | Third Wave Technologies, Inc. | Nucleic acid detection kits |
| US5338671A (en) | 1992-10-07 | 1994-08-16 | Eastman Kodak Company | DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody |
| SE501439C2 (sv) | 1993-06-22 | 1995-02-13 | Pharmacia Lkb Biotech | Sätt och anordning för analys av polynukleotidsekvenser |
| US6235470B1 (en) | 1993-11-12 | 2001-05-22 | The Johns Hopkins University School Of Medicine | Detection of neoplasia by analysis of saliva |
| WO1995014106A2 (en) | 1993-11-17 | 1995-05-26 | Id Biomedical Corporation | Cycling probe cleavage detection of nucleic acid sequences |
| AU1061395A (en) | 1993-11-30 | 1995-06-19 | Mcgill University | Inhibition of dna methyltransferase |
| US5851770A (en) | 1994-04-25 | 1998-12-22 | Variagenics, Inc. | Detection of mismatches by resolvase cleavage using a magnetic bead support |
| AU693198B2 (en) | 1994-04-25 | 1998-06-25 | Avitech Diagnostics, Inc. | Detection of mutation by resolvase cleavage |
| EP1260593A3 (en) | 1994-12-23 | 2003-12-03 | Dade Behring Inc. | Detection of nucleic acids by nuclease-catalyzed product formation |
| US5882867A (en) | 1995-06-07 | 1999-03-16 | Dade Behring Marburg Gmbh | Detection of nucleic acids by formation of template-dependent product |
| US5773258A (en) | 1995-08-25 | 1998-06-30 | Roche Molecular Systems, Inc. | Nucleic acid amplification using a reversibly inactivated thermostable enzyme |
| US5854033A (en) | 1995-11-21 | 1998-12-29 | Yale University | Rolling circle replication reporter systems |
| US5965408A (en) | 1996-07-09 | 1999-10-12 | Diversa Corporation | Method of DNA reassembly by interrupting synthesis |
| CA2239683A1 (en) | 1995-12-22 | 1997-07-03 | Dade Behring Marburg Gmbh | Homogeneous amplification and detection of nucleic acids |
| US5670325A (en) | 1996-08-14 | 1997-09-23 | Exact Laboratories, Inc. | Method for the detection of clonal populations of transformed cells in a genomically heterogeneous cellular sample |
| US5985557A (en) | 1996-01-24 | 1999-11-16 | Third Wave Technologies, Inc. | Invasive cleavage of nucleic acids |
| US5741650A (en) | 1996-01-30 | 1998-04-21 | Exact Laboratories, Inc. | Methods for detecting colon cancer from stool samples |
| US5891651A (en) | 1996-03-29 | 1999-04-06 | Mayo Foundation For Medical Education And Research | Methods of recovering colorectal epithelial cells or fragments thereof from stool |
| JP3898228B2 (ja) | 1996-04-12 | 2007-03-28 | ザ パブリック ヘルス リサーチ インスティチュート オブ ザ シティー オブ ニューヨーク インク | 検出プローブ、キット及びアッセイ |
| US6017704A (en) | 1996-06-03 | 2000-01-25 | The Johns Hopkins University School Of Medicine | Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids |
| US5786146A (en) | 1996-06-03 | 1998-07-28 | The Johns Hopkins University School Of Medicine | Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids |
| US5952178A (en) | 1996-08-14 | 1999-09-14 | Exact Laboratories | Methods for disease diagnosis from stool samples |
| US5928870A (en) | 1997-06-16 | 1999-07-27 | Exact Laboratories, Inc. | Methods for the detection of loss of heterozygosity |
| US6020137A (en) | 1996-08-14 | 2000-02-01 | Exact Laboratories, Inc. | Methods for the detection of loss of heterozygosity |
| US6096273A (en) | 1996-11-05 | 2000-08-01 | Clinical Micro Sensors | Electrodes linked via conductive oligomers to nucleic acids |
| AU7829398A (en) | 1997-06-09 | 1998-12-30 | University Of Southern California | A cancer diagnostic method based upon dna methylation differences |
| US6013459A (en) | 1997-06-12 | 2000-01-11 | Clinical Micro Sensors, Inc. | Detection of analytes using reorganization energy |
| DE19754482A1 (de) | 1997-11-27 | 1999-07-01 | Epigenomics Gmbh | Verfahren zur Herstellung komplexer DNA-Methylierungs-Fingerabdrücke |
| US6063573A (en) | 1998-01-27 | 2000-05-16 | Clinical Micro Sensors, Inc. | Cycling probe technology using electron transfer detection |
| WO1999040222A1 (en) | 1998-02-04 | 1999-08-12 | Variagenics, Inc. | Mismatch detection techniques |
| US6235502B1 (en) | 1998-09-18 | 2001-05-22 | Molecular Staging Inc. | Methods for selectively isolating DNA using rolling circle amplification |
| US7700324B1 (en) | 1998-11-03 | 2010-04-20 | The Johns Hopkins University School Of Medicine | Methylated CpG island amplification (MCA) |
| US6335193B1 (en) | 1999-04-15 | 2002-01-01 | Padmanabhan P Nair | Isolated colonocytes |
| CA2394921A1 (en) | 1999-12-07 | 2001-06-14 | Anthony P. Shuber | Supracolonic aerodigestive neoplasm detection |
| JP2004501666A (ja) | 2000-06-30 | 2004-01-22 | エピゲノミクス アーゲー | 薬理ゲノミクスのメチル化状態分析のための方法及び核酸 |
| DK1423528T3 (da) | 2000-09-01 | 2008-05-13 | Epigenomics Ag | Fremgangsmåde til bestemmelse af methyleringsgraden af bestemte cytosiner i genomisk DNA i sekvenskonteksten 5-CPG-3 |
| US7432050B2 (en) | 2001-10-05 | 2008-10-07 | Case Western Reserve University | Methods and compositions for detecting colon cancers |
| EP1497462A4 (en) | 2002-03-07 | 2007-11-07 | Univ Johns Hopkins Med | GENOMIC SCREEN ON EPIGENETIC MUTED CANCER ASSOCIATED GENES |
| US7662594B2 (en) | 2002-09-20 | 2010-02-16 | New England Biolabs, Inc. | Helicase-dependent amplification of RNA |
| US20040146868A1 (en) | 2003-01-24 | 2004-07-29 | Epigenomics Ag | Methods and nucleic acids for the analysis of CpG dinucleotide methylation status associated with the development of peripheral zone prostate cancer |
| WO2004083399A2 (en) | 2003-03-17 | 2004-09-30 | The Johns Hopkins University | Aberrantly methylated genes in pancreatic cancer |
| WO2005001141A2 (en) | 2003-06-23 | 2005-01-06 | Epigenomics Ag | Methods and nucleic acids for analyses of colorectal cell proliferative disorders |
| EP1660683B1 (en) | 2003-08-14 | 2017-04-19 | Case Western Reserve University | Methods and compositions for detecting colon cancers |
| US8346482B2 (en) | 2003-08-22 | 2013-01-01 | Fernandez Dennis S | Integrated biosensor and simulation system for diagnosis and therapy |
| DE602004018801D1 (de) * | 2003-09-04 | 2009-02-12 | Human Genetic Signatures Pty | Nukleinsäurenachweistest |
| WO2005023091A2 (en) | 2003-09-05 | 2005-03-17 | The Trustees Of Boston University | Method for non-invasive prenatal diagnosis |
| US20080039413A1 (en) | 2003-10-21 | 2008-02-14 | Morris David W | Novel compositions and methods in cancer |
| ES2437590T3 (es) | 2004-03-02 | 2014-01-13 | The Johns-Hopkins University | Mutaciones del gen de PIK3CA en cánceres humanos |
| US20100273151A1 (en) * | 2004-05-28 | 2010-10-28 | Fred Hutchinson Cancer Research Center | Genome-wide analysis of palindrome formation and dna methylation |
| US8114587B2 (en) | 2005-06-23 | 2012-02-14 | Ambergen, Inc. | Methods for the detection of colorectal cancer |
| US7851154B2 (en) | 2005-07-22 | 2010-12-14 | Simon Daniel Spivack | GC tag-modified bisulfite genomic DNA sequencing for continuous methylation spectra |
| EP1934369A2 (en) * | 2005-09-29 | 2008-06-25 | Epigenomics AG | Methods and nucleic acids for the analysis of gene expression associated with tissue classification |
| EP2423334A3 (en) | 2006-02-02 | 2012-04-18 | The Board of Trustees of The Leland Stanford Junior University | Non-invasive fetal genetic screening by digital analysis |
| JP4490382B2 (ja) | 2006-02-28 | 2010-06-23 | 富士フイルム株式会社 | 感熱転写受像シートおよびその製造方法 |
| WO2007116417A1 (en) | 2006-04-07 | 2007-10-18 | Silvia Sabbioni | Novel dna methylation markers useful for the diagnosis of neoplastic diseases |
| WO2007140319A1 (en) | 2006-05-26 | 2007-12-06 | Meltzer Stephen J | Methylated promoters as biomarkers of colon cancer |
| GB0700374D0 (en) | 2007-01-09 | 2007-02-14 | Oncomethylome Sciences S A | NDRG family methylation markers |
| US20080213870A1 (en) | 2007-03-01 | 2008-09-04 | Sean Wuxiong Cao | Methods for obtaining modified DNA from a biological specimen |
| CN101353695B (zh) | 2007-07-23 | 2013-05-01 | 上海市肿瘤研究所 | 尿沉淀dna甲基化谱式分析诊断膀胱癌的方法和试剂盒 |
| ES2522542T3 (es) | 2008-02-15 | 2014-11-17 | Mayo Foundation For Medical Education And Research | Detección de neoplasia a partir de una muestra de heces |
| KR101419980B1 (ko) | 2008-03-15 | 2014-07-15 | 홀로직, 인크. | 증폭 반응 도중에 핵산 분자를 분석하기 위한 조성물 및 방법 |
| WO2010062914A1 (en) | 2008-11-26 | 2010-06-03 | The Johns Hopkins University | Methods for identifying cancer risk |
| WO2010074924A1 (en) | 2008-12-23 | 2010-07-01 | University Of Utah Research Foundation | Identification and regulation of a novel dna demethylase system |
| EP2233590A1 (en) | 2009-01-28 | 2010-09-29 | AIT Austrian Institute of Technology GmbH | Methylation assay |
| DK2394170T3 (en) | 2009-02-03 | 2015-07-13 | Mdxhealth Sa | Methods for detecting colorectal cancer |
| JPWO2010113529A1 (ja) | 2009-04-03 | 2012-10-04 | 株式会社エイアンドティー | 大腸腫瘍の検出方法 |
| WO2011037936A2 (en) | 2009-09-24 | 2011-03-31 | Oregon Health & Science University | Detection of dna methylation of tal1, erg and/or cd40 to diagnose prostate cancer |
| US20120164110A1 (en) | 2009-10-14 | 2012-06-28 | Feinberg Andrew P | Differentially methylated regions of reprogrammed induced pluripotent stem cells, method and compositions thereof |
| US8709736B2 (en) | 2010-07-09 | 2014-04-29 | Medical Research Council | Biomarker for Barrett's Oesophagus |
| WO2011119934A2 (en) | 2010-03-26 | 2011-09-29 | Mayo Foundation For Medical Education And Research | Methods and materials for detecting colorectal neoplasm |
| US20130012410A1 (en) | 2010-03-29 | 2013-01-10 | Mayo Foundation For Medical Education And Research | Methods and materials for detecting colorectal cancer and adenoma |
| EP2407555A1 (en) * | 2010-07-14 | 2012-01-18 | Fundació Institut de Recerca Hospital Universitari Vall d'Hebron, Fundació Privada | Methods and kits for the diagnosis of prostate cancer |
| US9957570B2 (en) | 2010-10-04 | 2018-05-01 | The Johns Hopkins University | DNA hypermethylation diagnostic biomarkers for colorectal cancer |
| US8361720B2 (en) | 2010-11-15 | 2013-01-29 | Exact Sciences Corporation | Real time cleavage assay |
| US8715937B2 (en) | 2010-11-15 | 2014-05-06 | Exact Sciences Corporation | Mutation detection assay |
| US8916344B2 (en) | 2010-11-15 | 2014-12-23 | Exact Sciences Corporation | Methylation assay |
| EP2655664A2 (en) | 2010-12-21 | 2013-10-30 | Sloan-Kettering Institute for Cancer Research | Epigenomic markers of cancer metastasis |
| WO2012125721A2 (en) | 2011-03-14 | 2012-09-20 | Mayo Foundation For Medical Education And Research | Methods and materials for using the contents of phagocytes to detect neoplasms |
| AU2012253345B2 (en) | 2011-05-12 | 2016-10-20 | Exact Sciences Corporation | Isolation of nucleic acids |
| US8993341B2 (en) | 2011-05-12 | 2015-03-31 | Exact Sciences Corporation | Removal of PCR inhibitors |
| US8808990B2 (en) | 2011-05-12 | 2014-08-19 | Exact Sciences Corporation | Serial isolation of multiple DNA targets from stool |
| US8980107B2 (en) | 2011-05-12 | 2015-03-17 | Exact Sciences Corporation | Spin filter |
| WO2012167145A2 (en) | 2011-06-01 | 2012-12-06 | University Of Southern California | Genome-scale analysis of aberrant dna methylation in colorectal cancer |
| US20130022974A1 (en) * | 2011-06-17 | 2013-01-24 | The Regents Of The University Of Michigan | Dna methylation profiles in cancer |
| WO2012175562A2 (en) | 2011-06-21 | 2012-12-27 | University Of Tartu | Methylation and microrna markers of early-stage non-small cell lung cancer |
| CA2853645A1 (en) | 2011-11-30 | 2013-06-06 | National Cancer Center | Induced malignant stem cells |
| WO2013130880A1 (en) | 2012-02-28 | 2013-09-06 | The Johns Hopkins University | Hypermethylated gene markers for head and neck cancer |
| EP2825888A4 (en) | 2012-03-15 | 2015-09-23 | Mayo Foundation | METHOD AND MATERIALS FOR NONINVASIVE DETECTION OF COLORECTAL NEOPLASIA RELATED TO INFLAMMATORY ENDURANCE |
| JP6242388B2 (ja) | 2012-06-14 | 2017-12-06 | オーフス ユニバーシテットＡａｒｈｕｓ Ｕｎｉｖｅｒｓｉｔｅｔ | 前立腺ガンのバイオマーカー |
| CA2922005A1 (en) | 2012-09-27 | 2014-04-03 | Population Diagnostics, Inc. | Methods and compositions for screening and treating developmental disorders |
| US9994911B2 (en) | 2013-03-14 | 2018-06-12 | Mayo Foundation For Medical Education And Research | Detecting neoplasm |
| JP6369857B2 (ja) | 2013-05-29 | 2018-08-08 | シスメックス株式会社 | 肝細胞癌に関する情報の取得方法、ならびに肝細胞癌に関する情報を取得するためのマーカーおよびキット |
| MX2016004678A (es) * | 2013-10-24 | 2017-03-10 | Univ Georgetown | Composiciones para el tratamiento del cancer. |
| US20170298443A1 (en) | 2014-09-25 | 2017-10-19 | Moffitt Genetics Corporation | Prognostic tumor biomarkers |
| EP3034624A1 (en) | 2014-12-18 | 2016-06-22 | Hospital Clínic de Barcelona | Method for the prognosis of hepatocellular carcinoma |
| EP3037545A1 (en) * | 2014-12-23 | 2016-06-29 | The Provost, Fellows, Foundation Scholars, & the other members of Board, of the College of the Holy & Undiv. Trinity of Queen Elizabeth near Dublin | A DNA-methylation test for prostate cancer |
-
2018
- 2018-02-27 KR KR1020197025327A patent/KR102747156B1/ko active Active
- 2018-02-27 CN CN202410376668.XA patent/CN118147307A/zh active Pending
- 2018-02-27 EP EP18761075.3A patent/EP3589732A4/en active Pending
- 2018-02-27 WO PCT/US2018/019982 patent/WO2018160576A1/en not_active Ceased
- 2018-02-27 CN CN201880014641.9A patent/CN110431241B/zh active Active
- 2018-02-27 AU AU2018229294A patent/AU2018229294B2/en active Active
- 2018-02-27 JP JP2019547146A patent/JP7356349B2/ja active Active
- 2018-02-27 CA CA3054836A patent/CA3054836A1/en active Pending
- 2018-02-27 KR KR1020247042357A patent/KR20250006325A/ko active Pending
- 2018-02-27 US US15/906,932 patent/US10934592B2/en active Active
-
2021
- 2021-01-08 US US17/144,806 patent/US11697853B2/en active Active
-
2023
- 2023-05-24 US US18/323,031 patent/US20230416840A1/en active Pending
- 2023-06-07 JP JP2023094124A patent/JP7674415B2/ja active Active
-
2024
- 2024-09-13 AU AU2024219684A patent/AU2024219684A1/en active Pending
-
2025
- 2025-04-24 JP JP2025072146A patent/JP2025114618A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| US20180245163A1 (en) | 2018-08-30 |
| CN118147307A (zh) | 2024-06-07 |
| JP7356349B2 (ja) | 2023-10-04 |
| US11697853B2 (en) | 2023-07-11 |
| US10934592B2 (en) | 2021-03-02 |
| JP7674415B2 (ja) | 2025-05-09 |
| EP3589732A1 (en) | 2020-01-08 |
| KR20190140901A (ko) | 2019-12-20 |
| JP2023123530A (ja) | 2023-09-05 |
| AU2018229294A1 (en) | 2019-09-26 |
| JP2025114618A (ja) | 2025-08-05 |
| CN110431241A (zh) | 2019-11-08 |
| US20230416840A1 (en) | 2023-12-28 |
| AU2024219684A1 (en) | 2024-10-10 |
| AU2018229294B2 (en) | 2024-06-13 |
| WO2018160576A1 (en) | 2018-09-07 |
| KR102747156B1 (ko) | 2024-12-26 |
| US20210130911A1 (en) | 2021-05-06 |
| CA3054836A1 (en) | 2018-09-07 |
| JP2020513795A (ja) | 2020-05-21 |
| CN110431241B (zh) | 2024-04-19 |
| EP3589732A4 (en) | 2021-05-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| AU2022291502B2 (en) | Detecting hepatocellular carcinoma | |
| JP7674415B2 (ja) | 前立腺癌検出 | |
| US20240191308A1 (en) | Detecting gastric neoplasm | |
| US20230212691A1 (en) | Detecting colorectal neoplasia | |
| US20230028856A1 (en) | Detecting gastrointestinal neoplasms | |
| US20230357852A1 (en) | Detecting non-hodgkin lymphoma | |
| HK40103516A (en) | Detecting hepatocellular carcinoma |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A107 | Divisional application of patent | ||
| PA0104 | Divisional application for international application |
Comment text: Divisional Application for International Patent Patent event code: PA01041R01D Patent event date: 20241220 Application number text: 1020197025327 Filing date: 20190828 |
|
| PG1501 | Laying open of application | ||
| A201 | Request for examination | ||
| PA0201 | Request for examination |
Patent event code: PA02012R01D Patent event date: 20250117 Comment text: Request for Examination of Application |