KR102659293B1 - Stable composition for controlling pests comprising nanoparticles of calcium oxide and method for controlling pests by using the same - Google Patents

Abstract

The present invention relates to a pest control composition having excellent stability against high temperature or moisture and a pest control method using the same, and more specifically, to an oil-based formulation to solve the stability problem during storage of the pest control composition. and relates to a composition for controlling pests containing calcium oxide nanoparticles.

Description

Stable composition for controlling pests comprising calcium oxide nanoparticles and method for controlling pests using the same {Stable composition for controlling pests comprising nanoparticles of calcium oxide and method for controlling pests by using the same}

The present invention relates to a pest control composition having excellent stability against high temperature or moisture and a pest control method using the same.

In order to control diseases and pests that damage crops, the use of chemical pesticides has increased and the spraying area has become wider as the size of cultivated crops has increased along with the development of agricultural technology. The emergence of organic synthetic pesticides after World War II marked a revolutionary turning point in two aspects: increasing crop yields through agricultural pest control and improving human health through sanitary pest control. The amount of pesticides used to control pests in Korea is increasing every year, and among them, organophosphorus and carbamate types account for about 90% of the total amount used.

However, decades of continued use and abuse of these existing organic synthetic pesticides have resulted in various side effects such as the emergence of resistant pests, toxicity to livestock, disruption of biological control by natural enemies, residual poison, and environmental pollution. Recently, these synthetic pesticides have been introduced. As interest in side effects and environmental impacts increases, the use of synthetic pesticides is gradually being restricted. Accordingly, biological control is being actively researched as part of the development of next-generation pesticides that can replace synthetic pesticides, but biological control uses microorganisms, natural enemies, or natural extracts and is not easy to handle, which is an obstacle to commercialization.

In this regard, microbial crop protection agents that are biologically controlled using entomopathogenic molds (fungi) have the advantage of minimizing resistance and environmental pollution by using fungal spores, unlike the chemical pesticides mentioned above. However, this is an important factor in commercialization. Research on mass production and stabilized formulation is still insufficient. In particular, entomopathogenic fungi ( Beauveria bassiana ) are currently sold in granular form to control pests such as thrips, which absorb the sap of plant leaves and take away nutrients, thereby reducing the value of feed. However, preparations using these entomopathogenic molds must be prepared by separating spores produced through solid culture from the culture medium and then mixing them with water in the form of a liquid preparation or wettable powder for spray application. However, when mixed with water, the spores are 1. It dies within a week, and in powder form, it is somewhat stable at room temperature, but it has the disadvantage of dying within a week when exposed to high temperatures (above 50℃). It is common for crop protection agents to be exposed to temperatures above 40℃ during the distribution process.

Due to these problems, conventional entomopathogenic fungal preparations have many problems with their physical properties and are inconvenient to use, and their expiration date is one year, making it difficult for business to export and sell them to other countries. Therefore, if storage stability is improved, a large ripple effect is expected.

In this regard, Korean Patent Publication No. 10-2016-0084968 discloses the Boveria bassiana JEF007 strain, an entomopathogenic fungus with insect pathogenicity and excellent spore-forming ability, and a liquid preparation for controlling agricultural pests using the same, and the strain using a filterable bag. The manufacturing method of the liquid formulation containing is disclosed, but storage stability is not mentioned.

Therefore, there is an urgent need to develop a new commercially available pest control agent that is harmless to the human body, environmentally friendly, easy to handle, and has excellent storage stability against high temperature and moisture.

Korean Patent Publication No. 10-2016-0084968 (2016.07.15)

Under this background, the present inventors have conducted repeated research to solve the stability problem of pathogenic mold spores being inhibited by various factors such as moisture and high temperature. As a result, when combining silicone oil and calcium oxide nanoparticles with pathogenic mold agents, The present invention was completed by confirming that a pest control composition and pest control method with excellent stability can be provided by significantly reducing the problem of decreased stability of pathogenic fungal preparations due to high temperature or moisture.

As an example, a composition for controlling pests is provided, comprising pathogenic mold or its spores, silicone oil, and calcium oxide nanoparticles.

As another example, a method for controlling pests is provided, comprising the step of applying the composition for controlling pests to pests, their habitats, or crops vulnerable to pests.

As another example, a method for stabilizing a composition for controlling pests is provided, which includes mixing and storing pathogenic mold or its spores, silicone oil, and calcium oxide nanoparticles.

In one aspect, the present invention relates to a composition for controlling pests, comprising pathogenic mold or its spores, silicone oil as an oil solvent, and calcium oxide nanoparticles.

As used herein, “pathogenic fungi” refers to fungi that cause disease in organisms and mainly proliferate through spores. “Entomopathogenic fungi,” which is a preferred example of a pathogenic fungus to which the present invention can be applied, refers to a fungus that taxonomically mainly belongs to the Imperfecta and Zygomycetes and has pest control activity. Unlike entomopathogenic bacteria or viruses that are infected through ingestion of the host, entomopathogenic fungi germinate by attaching to the outer shell of the host insect and then pass through the cuticle layer using an attachment tool, which is an invasion tool, or a germ tube sprouted from the spore. It directly passes through the cuticle layer and invades the inside of the insect. At this time, physical action and enzymatic action such as chitinase, protease, lipase, and esterase occur together. The fungus that invades the inside of an insect secretes toxic substances as it proliferates inside the insect's body, blocking the insect's immune system and killing the host insect, so it can be used as an insecticide.

More than 700 types of entomopathogenic fungi have currently been reported, and as long as they produce spores and have an insecticidal effect on insects, they can be used without restrictions. For example, Beauveria , Isaria , Lecanicillium , Nomuraea , Celletotrichum , and Fusarium . Genus Phytophthora , Sclerotinia , Metarhizium , Paecilomyces , Verticillium , Trichoderma , Hirsutella ) genus, Aspergillus genus, Aschersonia genus , or Examples include fungi of the genus Metarrhizium . Specifically, Beauveria bassiana, Metarhizium anisopliae , Verticillium lecanii, Aschersonia aleyrodis or Nomuraea rileyi. ) can be exemplified. Boveria bassiana used in one example of the present application is a strain that has excellent insecticidal effects against saw-legged stink bugs, yellow flower thrips, etc.

These pathogenic molds or their spores may be appropriately included in an amount that can exhibit a pest control effect, but are preferably included in an amount of 1 to 90% by weight, more specifically, 1 to 30% by weight, based on the total weight of the pest control composition. It may be included in weight%, more specifically 5 to 20% by weight, more specifically 7 to 15% by weight, and most specifically 8 to 10% by weight, but is not limited thereto.

Spores are gametes produced by molds for asexual reproduction. They can develop into adults without forming a zygote, and preferably contain conidia. The composition for controlling pests herein includes entomopathogenic fungi or spores thereof, which is understood to include spores, hyphae, mycelium, fruiting bodies, sporozoites, or equivalents thereof. When a spore touches the outer shell of an insect, a germ tube is created from the spore. The germ tube grows until the nutrients in the spore are depleted and begins to penetrate the cuticle layer of the insect. As the germ tube penetrates, an enzyme that dissolves the cuticle layer is released. Secrete.

In one specific example, Boveria bassiana ERL836 was used as the raw material of the pest control composition according to the present invention, but the present invention is not limited thereto.

In the present invention, in order to solve the problem of stability due to high temperature or moisture in particular, calcium oxide (CaO) nanoparticles having a specific particle size, specifically 70 nm or less, are included as an additive. The “calcium oxide” is also called quicklime, and its chemical formula is CaO. The pure form is an equiaxed white crystal with a melting point of 2,570°C. When left in the air, it absorbs moisture and carbon dioxide and decomposes into calcium hydroxide (slaked lime) and calcium carbonate. Additionally, it is a substance that generates heat when exposed to water and becomes calcium hydroxide. This calcium oxide is produced by heating limestone or calcium carbonate CaCO ₃ to about 900°C or higher. These calcium oxide nanoparticles can control the moisture content in the formulation to a level that maximizes stability, for example, 1% by weight or less, more specifically 0.5% by weight or less, 0.3% by weight or less, most specifically. It can be adjusted to a level of 0.1% by weight or less, but is not limited to this.

In one embodiment, the particle diameter of the calcium oxide nanoparticles may be 70 nm or less, for example, 20 to 70 nm, 30 to 70 nm, 40 to 70 nm, or 50 to 70 nm, but is not limited thereto. Calcium oxide nanoparticles having the above particle size have a slower sedimentation rate compared to calcium oxide microparticles having a general size and can maintain dispersion for a long time. As a result of comparing the degree of precipitation of calcium oxide particles by height ratio over time using calcium oxide nanoparticles and microparticles in the same composition as in the embodiment of the present invention, the nanoparticles maintained dispersion for about 1000 times longer until precipitation. It was shown that (Figure 2).

In addition, the calcium oxide nanoparticles may be included in an amount of 1 to 50% by weight, more specifically, 1 to 30% by weight, 3 to 20% by weight, or 4 to 10% by weight, based on the total weight of the pest control composition. It may be possible, but it is not limited to this.

Furthermore, the composition for controlling pests according to the present invention is characterized by containing silicone oil as an oily solvent. In the case of silicone oil, there is little change in viscosity over a wide temperature range, excellent heat and cold resistance, and it is also colorless, transparent, non-volatile, and harmless to the human body, so it maintains the stability of pest control compositions better than other oil solvents. make it possible Preferably, the moisture content in the silicone oil is 0.1% or less.

In addition, based on the total weight of the pest control composition, silicone oil may be included in an amount of 30 to 95% by weight, more specifically, 60 to 95% by weight, 60 to 90% by weight, 70 to 90% by weight, or 80 to 87% by weight. It may be weight percent, but is not limited thereto. Furthermore, the silicone oil may be selected to have an appropriate viscosity as needed, but preferably may have a viscosity of 0.1 to 1000 cP at a temperature of 25°C, more specifically 0.5 to 500 cP, most specifically. may represent a viscosity of 0.5 to 200 cP, but is not limited thereto. In one specific example, dimethyl silicone oil KF96-100cs (100 cP viscosity) was used as the silicone oil in the pest control composition according to the present invention.

The composition for controlling pests of the present invention may also include a surfactant. Surfactants are amphiphilic substances that have both hydrophilic and lipophilic molecular groups in the molecule, and have cleaning power, dispersing power, emulsifying power, solubilizing power, wetting power, sterilizing power, foaming power and/or penetrating power, and are used for controlling pests according to the present invention. It can act to hydrate, suspend or disperse pathogenic molds or spores in the composition to effectively exert pest control effects.

Surfactants include, but are not limited to, hydrophilic surfactants, lipophilic surfactants, or mixtures thereof. For example, the hydrophilic lipophilic balance (HLB) value of the surfactant may be about 1 to 20, about 1 to 17, or 2 to 12. In one embodiment, the HLB of the surfactant may be 1 to 7, or 10 to 17, or 10 to 20. In another embodiment, the HLB of the surfactant may be 1 or more to less than 7, 10 or more to less than 17, or 10 to less than 20. The above HLB range exemplifies an appropriate range in which the surfactant can effectively dissolve in an oily solvent and exhibit a surfactant effect, but the scope of the present invention is not limited thereto. HLB is an indicator of the balance between hydrophilicity and lipophilicity (hydrophobicity) of a surfactant. It is understood that the larger the HLB, the larger the hydrophilicity ratio, and the smaller the HLB, the larger the lipophilicity (hydrophobicity) ratio. The surfactant may also be nonionic, anionic, cationic, or zwitterionic (or amphoteric).

Examples of cationic surfactants that can be used herein include Dodecyltrimethylammonium bromide (DTAB) or Cetyltrimethylammonium bromide (CTAB), and examples of anionic surfactants include dioctyl sulfosuccinate sodium (DSS), sodium dodecyl sulfate (SDS), and LDS. (lithium dodecyl sulfate), SDBS (sodium dodecyl benzene sulfonate), or SDSA (sodium dodecylsulfonate), etc. Examples of amphoteric surfactants include lecithin, and examples of nonionic surfactants include Lauryl PEG/PPG-18/18 Methicone, Cetyl Diglyceryl Tris(Trimethylsiloxy)silylethyl Dimethicone, PEG-9 Dimethicone (PEG-9 dimethicone), PEG-10 dimethicone (PEG-10 dimethicone), Sorbitan monooleate, Octylphenol Ethoxylate, polyethylene glycol, alkyl polyglucoside, silane poly Alkylene oxide (copolymer), nonylphenol ethoxylate, tristyrylphenol ethoxylate, polyoxyethylene alkyl ether, polyoxyethylene alkylphenyl ether, alkylallyl formaldehyde condensed polyoxyethylene ether, polyoxyethylene poly. Oxypropylene block polymer, polyoxyethylene polyoxypropylene alkyl ether, polyoxyethylene ether of glycerin ester, polyoxyethylene ether of sorbitan ester, polyoxyethylene ether of sorbitol ester, polyethylene glycol fatty acid ester, glycerin ester, polyglycerin ester , sorbitan ester, propylene glycol ester, sucrose ester, aliphatic acid alkanol amide, polyoxyethylene fatty acid amide, alcohol ethoxylate, amine ethoxylate, glucoside, glucamide, polyethylene glycol, poly(ethylene glycol-co). -Propylene glycol), cetyl alcohol, stearyl alcohol, cetostearyl alcohol, oleyl alcohol, octaethylene glycol monododecyl ether, pentaethylene glycol monododecyl ether, polyoxypropylene glycol alkyl ether, decyl glucoside, lauryl glucoside , octyl glucoside, polyoxyethylene glycol octylphenol ether, nonoxynol-9, glycerol alkyl ester, glyceryl laurate, polyoxyethylene glycol sorbitan alkyl ester, polysorbate, sorbitan alkyl ester, span, cocamide MEA, cocamide DEA, dodecyldimethylamine oxide, block copolymers of polyethylene glycol, polypropylene glycol, and silane polyalkylene oxides (copolymers), or polyoxyethylene alkyl amides, for example , Triton (registered trademark) X-114, X-102, X-100, X-45, brij30), polyoxyethylene-23-lauryl ether (brij35), polyoxyethylene-2-cetyl ether (brij52), polyoxyethylene-10-cetyl ether (brij56), polyoxyethylene-20-cetyl ether (brij58) ), polyoxyethylene-2-stearyl ether (brij72), polyoxyethylene-10-stearyl ether (brij76), polyoxyethylene-2-oleyl ether (brij93), or polyoxyethylene-10-oleyl ether (brij97), PEG400 dioleate (PEG-8 dioleate), etc., but is not limited thereto.

In a preferred embodiment, the surfactant is at least one selected from the group consisting of lauryl PEG/PPG-18/18 methicone, cetyl diglyceryl tris(trimethylsiloxy)silylethyl dimethicone, and PEG-9 dimethicone. may, but is not limited to this. In a specific example of the present application, a formulation containing Dow 5200 (lauryl PEG/PPG-18/18 methicone) was prepared, but the scope of the present invention is not limited thereto.

In a preferred embodiment, the surfactant may be included in an amount of 0.001 to 90% by weight, more specifically, 0.001 to 50% by weight, 0.005 to 20% by weight, 0.005 to 4% by weight, 0.01% by weight, based on the total weight of the pest control composition. It may be from 4% by weight, 0.01 to 2% by weight, or 0.05 to 1.0% by weight, but is not limited thereto. However, if it is less than the above range, there may be a problem that the surfactant effect is not sufficiently displayed, and if it exceeds the above range, there may be a problem that the surfactant is not fully dissolved in the solvent and precipitation or precipitation occurs.

The composition for controlling pests according to the present invention can be prepared according to a known method for producing a composition for controlling pests. For example, it can be prepared by combining pathogenic mold or its spores, silicone oil and calcium oxide nanoparticles, and optionally a surfactant. Pathogenic mold or its spores can be pulverized to have an appropriate particle size if necessary, and a liquid sample is prepared by adding surfactant and calcium oxide nanoparticles to an oily solvent, and then mixing the pulverized pathogenic mold or its spores to form a sample. A composition for controlling pests according to the invention can be prepared. The composition for controlling pests according to the present invention can be formulated in various forms, but is preferably in the form of a sprayable liquid concentrate.

The composition for controlling pests according to the present invention exhibits excellent stability at high temperature and moisture. Specifically, the composition for controlling pests according to the present invention is at 50°C, or 54°C or higher, for example, at 50°C to 60°C, 50°C to 55°C, or 50°C to 54°C, the insect pathogenic mold or More than 1.0% of its spores can remain viable for more than a week. In addition, the pathogenicity at room temperature, or 50°C, or 54°C or higher, for example, 50°C to 60°C, 50°C to 55°C, or 50°C to 54°C, for at least 1 week, preferably 5 weeks. It is characterized by maintaining . In addition, at room temperature, or 50 ℃, or 54 ℃ or higher, for example, 50 ℃ to 60 ℃, 50 ℃ to 55 ℃, or 50 ℃ to 54 ℃, preferably more than the effective number of bacteria for 5 weeks, specific It is characterized by maintaining a bacterial count of 1.0E+05 CFU/mL or more.

In a specific example, after pulverizing the spores uniformly, KF96-100cs, a silicone oil, and calcium oxide nanoparticles were separately mixed, dispersed through ball milling, and Dow 5200 as a surfactant was added to the dispersion. After introduction, the composition was vortexed for 30 seconds and then sterilized, and the raw agent was added and vortexed for 1 minute to prepare a pest control composition according to the present invention. When compared with the comparative example consisting of only the raw material, the treatment It was confirmed that excellent storage stability was observed for a period of 5 weeks (see Figure 1).

In another aspect, the present invention includes silicone oil as an oil solvent and calcium oxide nanoparticles as an additive in a pest control composition containing pathogenic mold or its spores and a surfactant as a dispersant to prevent entomopathogenic mold or its spores. It is about ways to increase the survival rate of. Preferably, the silicone oil and calcium oxide nanoparticles maintain the entomopathogenic mold or its spores in a sufficiently low state for a long period of time, thereby increasing the survival rate of the entomopathogenic mold or its spores compared to a state in which the moisture content is high. It is characterized by an increase.

In another aspect, the present invention relates to a method for stabilizing a composition for controlling pests, comprising the step of mixing and storing pathogenic mold or its spores, silicone oil, and calcium oxide nanoparticles.

In another aspect, the present invention relates to a method for controlling pests by applying the pest control composition according to the present invention to pests, their habitats, or crops vulnerable to pests.

The composition of the present invention can be used in a method for controlling pests by applying the pest control composition according to the present invention to pests, their habitats, or crops vulnerable to pests.

Examples of pests that can be controlled by the composition of this embodiment include arthropods such as insects and mites, especially harmful arthropods such as harmful insects and harmful mites, and may include:

- Hemiptera: Delphacidae, such as Laodelphax striatellus , Nilaparvata lugens and Sogatella furcifera ; Deltocephalidae, such as Nephotettix cincticeps and Nephotettix virescens ; Aphids (Aphididae), such as cotton aphid ( Aphis gossypii ), peach aphid ( Myzus persicae ), cabbage aphid ( Brevicoryne brassicae ), potato aphid ( Macrosiphum euphorbiae ), Aulacorthum solani , millet aphid ( Rhopalosiphum padi ) and tangerine aphid ( Toxoptera citricidus ); Stink bugs (Pentatomidae), such as grass-colored stink bug ( Nezara antennata ), saw-legged stink bug ( Riptortus clavetus ), long-legged stink bug ( Leptocorisa chinensis ), spiny-spotted stink bug ( Eysarcoris parvus ), sycamore stink bug ( Haliomorpha mista ) and blind stink bug. ( Lyuslineolaris ); Whiteflies ( Aleyrodidae ), such as greenhouse whiteflies ( Trieurodes vaporariorum ), tobacco whiteflies ( Bemisiatabaci ) and silver leaf whiteflies ( Bemisia argentifolii ); Coccidae , such as California red scales ( Aonidiella aurantii ), San Jose scales ( Comstockaspis perniciosa ), arrow scales ( Unaspiscitri ), ruby scales ( Ceroplastes rubens ), and Icerya purchasi ; Tingidae; Psyllidae , etc.;

- Lepidoptera: Pyralidae , such as Chilo suppressalis , Tryporyzaincertulas , Cnaphalocrocis medinalis , Notarchaderogata , Plodia interpunctella , etc. Moth ( Ostrinia furnacalis ), European light moth ( Ostrinia nubilaris ), cabbage shoot moth ( Hellula undalis ), and lawn moth ( Pediasiateterrellus ); Noctuidae , such as Spodoptera litura , Spodoptera exigua , Pseudaletia separata , Mamestra brassicae , Agrotis ipsilon, and Agrotis ipsilon . Butterflies ( Plusianigrisigna ), Thoricoplusia spp., Heliothis spp., and Helicoverpa spp.; White butterflies ( Pieridae ), such as cabbage white butterflies ( Pierisrapae ); Tortricidae , such as Adoxophyes spp., Peach shoot moth ( Grapholitamolesta ), Bean moth ( Leguminivora glycinivorella ), Red bean moth ( Matsumuraeses azukivora ), Apple Adoxophyesorana fasciata , Tea moth Adoxophyes honmai , Homona magnanima , Archipsfuscocupreanus , and Cydia pomonella ; Gracillariidae , such as Caloptilia theivora and Phyllonorycter ringoniella ; Carposinidae , such as peach heartworm ( Carposina niponensis ); Oyster moths ( Lyonetiidae ), such as silver-spotted oyster moths ( Lyonetia spp.); Lymantriidae , such as Lymantria spp. and Euproctis spp.; House moths ( Yponomeutidae ), such as diamondback moths ( Plutella xylostella ); Gelechiidae, such as Pectinophora gossypiella and Phthorimaea operculella ; Arctiidae , such as Hyphantria cunea ; and grain moths ( Tineidae ), such as clothes moths ( Tinea translucens ), etc.;

- Thysanoptera: Yellow flower thrips ( Frankliniella occidentalis ), cucumber thrips ( Thrips palmi ), convex thrips ( Scirthrips dorsalis ), green onion thrips ( Thripstabaci ), Taiwan thrips ( Frankliniella intonsa ), and tobacco thrips. bugs ( Frankliniella fusca ), etc.;

- Diptera: Agromyzidae, such as Hylemyaantiqua , Hylemyaplatura , Agromyza oryzae , Hydrellia griseola , Chlorops oryzae , American leaf oyster fly ( Liriomyza trifolii ); Melon fly ( Dacus cucurbitae ) and Mediterranean fruit fly ( Ceratitis capitata ), etc.;

- Coleoptera: 28 spotted ladybug ( Epilachna vigintioctopunctata ), cucumber leaf beetle ( Aulacophora femoralis ), flea leaf beetle ( Phyllotreta striolata ), rice leaf beetle ( Oulema oryzae ), rice root weevil ( Echinocnemus squameus ), rice water weevil ( Lissorhoptrus oryzophilus ), cotton weevil ( Anthonomus grandis ), red bean weevil ( Callosobruchus chinensis ), hunting weevil ( Sphenophorus venatus ), Japanese beetle ( Popillia japonica ), copper beetle ( Anomala cuprea ), corn leaf beetle ( Diabrotica spp.), Colorado potato beetle ( Leptinotarsa decemlineata ), green bugs ( Agriotes spp.), and cigarette bugs ( Lasioderma serricorne ), etc.;

- Orthoptera: ground locust ( Gryllotalpa africana ), rice locust ( Oxya yezoensis ), and rice locust ( Oxya japonica ), etc.;

- Hymenoptera: Athalia rosae , Acromyrmex spp., and Solenopsis spp.;

- Acarina: mites (Tetranychidae), such as spotted mites ( Tetranychus urticae ), tangerine mites ( Panonychus citri ) and tree mites ( Oligonychus spp.); Eriophyidae , such as Aculops pelekassi ; Dust mites ( Tarsonemidae ), such as tea dust mite ( Polyphagotarsonemus latus ); Tenuipalpidae ; tuckerellidae ; Acaridae , such as Tyrophagus putrescentiae ; Dust mites ( Pyroglyphidae ), such as Dermatophagoides farinae and Dermatophagoides ptrenyssnus ; Cheyletidae , such as Cheyletus eruditus , Cheyletus malaccensis and Cheyletus moorei;

- Nematodes ( Nematodes ): Rice leaf nematode ( Aphelenchoides besseyi ) and strawberry nematode ( Nothotylenchusacris ), etc.

An example of a method for controlling pests using the compositions of the present disclosure is to spray the compositions of the present disclosure onto the target pest or pest habitat. “Pest habitat” means not only the area where the pest actually lives, but also at least the area through which the pest passes or moves. It also refers to areas where pests are assumed to be habitats or passageways, as well as areas from which pests are preferably eradicated.

Examples of plants to which the compositions or methods herein can be applied include:

- Crops: corn, rice, wheat, barley, rye, oats, sorghum, cotton, soybeans, peanuts, buckwheat, sugar beets, rapeseed, sunflower, sugarcane and tobacco, etc.;

- Vegetables: Solanaceae vegetables (eggplant, tomatoes, green peppers, peppers, potatoes, etc.), Cucurbits vegetables (cucumber, pumpkin, zucchini, watermelon, melon, etc.), Cruciferous vegetables (Japanese radish, turnip, horseradish, kohlrabi, Chinese cabbage, etc.) Cabbage, black mustard, broccoli, cauliflower, rapeseed, etc.), Asteraceae vegetables (burdock, mugwort, artichoke, lettuce, etc.), Asteraceae vegetables (green onions, onions, garlic, asparagus, etc.), Apiaceae vegetables (carrots, parsley, celery, etc.) , parsnip, etc.), pigweed vegetables (spinach, Swiss chard, etc.), Lamiaceae vegetables (perilla, mint, basil, etc.), strawberries, sweet potatoes, yams, taro, etc.;

- Fruit trees: human fruits (apples, pears, Japanese pears, Chinese quinces, quinces, etc.), stone fruits (peaches, plums, nectarines, loquats, cherries, apricots, prunes, etc.), citrus fruits (Wenzhou tangerines, oranges, lemons, limes, etc.) grapefruit, etc.), nuts (chestnuts, walnuts, hazelnuts, almonds, pistachios, cashews, macadamia nuts, etc.), berries (blueberries, cranberries, blackberries, raspberries, etc.), grapes, persimmons, olives, loquats, bananas, coffee, jujubes , coconut, oil palm, etc.;

- Trees other than fruit trees: green tea, mulberries, flower trees (azalea, camellia, hydrangea, camellia, Japanese star anise, cherry tree, tulip tree, crape myrtle, osmanthus, etc.), street trees (ash tree, birch) Trees, dogwood, eucalyptus, ginkgo, lilac, maple, oak, poplar, birch, Taiwan fir, sycamore, zelkova, Japanese arborvitae, fir, hemlock, juniper, pine, spruce, yew. , spruce, elm, horse chestnut, etc.), coral tree, Podocarpus, cedar, cypress, croton, Euonymus japonicus, Photiniaglabra , etc.;

- Lawn: grass (Korean grass (zoysiagrass), golden grass ( Zoysiamatrella ), etc.), umbrella grass ( Cynodondactylon , etc.), bran (bentgrass) (white bran ( Agrostisalba ), creeping bent grass, hiland bent grass) bent, etc.), bluegrass (meadow grass, bird grass, etc.), fescue (tall fescue, chewing fescue, creeping red fescue) red fescue, etc.), ryegrass (darnel, ryegrass, etc.), orchard grass, timothy grass, etc.;

- Other: Flowers (roses, carnations, chrysanthemums, prairie gentian), gypsophila, gerbera, marigold, salvia, petunia, verbena, tulips , asters, gentians, lilies, pansies, cyclamen, orchids, convallaria, lavender, stock, ornamental cabbage, primula, poinsettia, gladiolus. ), cattleya, daisy, cymbidium, begonia, etc.), biofuel plants (Jatropha, safflower, camelina, switchgrass, silver grass ( Miscanthus, reed canary grass, giant reed, kenaf, cassava, willow, etc.), ornamental plants, etc.

The pest control composition of the present invention can be applied to plants or plant cultivation areas for pest control. As used herein, plant includes plant stems or leaves, plant flowers, plant fruits, plant seeds, etc. Examples of application methods include application to the stems and leaves of plants, such as foliar application; Application to plant seeds; and applications to areas where plants are cultivated, such as soil applications and submerged applications.

The pest control composition according to the present invention uses silicone oil as an oil solvent and calcium oxide nanoparticles as an additive, and excellently improves the stability problems caused by high temperature and moisture, which are problems in conventional pest control compositions, for pest control use. It is very effective in reducing the decrease in potency due to temperature and moisture when storing the composition.

Figure 1 is a graph showing the spore concentration when treated for 5 weeks at room temperature and 54°C storage conditions to confirm the stability of examples and comparative examples according to the present invention.
Figure 2 is a graph showing the degree of precipitation of calcium oxide nanoparticles used in the present invention and general calcium oxide microparticles as a ratio of height over time.

Hereinafter, the present invention will be described in detail through examples and experimental examples. However, the following examples and experimental examples are merely illustrative of the present invention, and the scope of the present invention is not to be construed as limited to the following examples and experimental examples. .

preparation of ingredients

Beauveria bassiana ERL836 (KCCM11506P) was used as the pathogenic spore strain. To cultivate the fungus, solid culture was performed using grain as a medium raw material. For this purpose, first, spores of Boveria bassiana ERL836 stored at -70°C were plated on Potato Dextrose Agar and cultured at 25°C for 7 days. Spores were recovered, inoculated into millet, and cultured for 1 week to perform seed culture. For the main culture, the millet was immersed in water to ensure it contained sufficient moisture, then placed in a steamer and steamed at a high temperature of 100°C or higher for more than 10 minutes. The steamed millet was cooled at room temperature, and the seed culture was inoculated and mixed. The mixture was cultured at 25°C for more than 5 days, and the culture was dried at low temperature to recover spores. If the number of spores in the original product was more than ¹

Silicone oil (KF96-100cs) was used as an oil phase solvent, Dow 5200 (lauryl PEG/PPG-18/18 methicone, nonionic, HLB=6.8) was used as a surfactant, and calcium oxide with a particle size of 50-70 nm was used. Nanoparticles were self-synthesized and used.

Experimental Example 1: Preparation and storage stability analysis of the composition for controlling pathogenic pests according to the present invention

A composition for controlling pathogenic pests containing silicone oil and calcium oxide nanoparticles according to the present invention was prepared according to the following method.

Test Methods:

i) The dried culture of Boveria bassiana ERL836, the source of pathogenic spores, was ground uniformly with a mortar and pestle.

ii) Samples of raw agent, oil, surfactant, and calcium oxide nanoparticles were prepared according to the conditions in Table 1 below.

Unit: mass ratio (wt%) sample Original title silicone oil Surfactant (Dow 5200) Calcium oxide nanoparticles Example 1 9.1 85 1.4 4.5 Comparative Example 1 100 0 0 0

- Original title: ERL836 (Beauveria bassiana) - Oil: Silicone oil (KF96-100cs)

- Surfactant: Dow 5200 (lauryl PEG/PPG-18/18 methicone)

- Calcium oxide nanoparticles: Calcium oxide with a particle size of 50-70 nm.

iii) Samples prepared according to the content ratios in Table 1 were prepared as follows.

- Example 1: Oil and calcium oxide nanoparticles were mixed and dispersed through ball milling, then a surfactant was added and vortexed for 30 seconds. Afterwards, sterilization was performed at 80°C for 30 minutes, the raw agent was added, and vortexing was performed for 1 minute to prepare a composition according to the example.

- Comparative Example 1: A 100% by weight sample of the original drug was prepared.

iv) Each test sample was stored at room temperature (25°C) and 54°C, respectively.

v) Each sample was stored at 1-week intervals for a total of 6 weeks, and 1 ml of sample was drawn at 1-week intervals and diluted in an aqueous solution (NaCl 0.85 wt% and dioctyl sulfosuccinate sodium 0.27 wt%), After dispersing by vortexing for 1 minute, it was spread on PDA (Tartaric acid) medium and cultured in an incubator at 25°C.

vi) The number of active spore colonies was counted through a viable spore count (unit: CFU (Colony forming unit)/mL).

The test results are shown in Figure 1. As can be seen in Figure 1, in Comparative Example 1 using the original agent itself, no spores were observed under storage conditions at 54°C, whereas the pest control composition of Example 1 contained calcium oxide nanoparticles and was stored at 54°C. It was confirmed that the storage stability was significantly improved by showing storage stability of more than 1.0E+05 cfu/mL, which was effective until the 5th week under the storage conditions.

Experimental Example 2: Comparison of dispersibility according to calcium oxide particle size

The composition of Example 1 prepared in Experimental Example 1 and the composition of Comparative Example 2, which had the same composition as Example 1 but contained calcium oxide microparticles with a particle size of 3 to 5 μm instead of calcium oxide nanoparticles, were left at room temperature. The degree of precipitation of calcium oxide particles was observed over time and expressed as a ratio of height over time.

As a result, as shown in Figure 2, the composition of Example 1 of the present invention was found to maintain dispersion for about 1000 times longer than the composition of Comparative Example 2 due to the slower precipitation of calcium oxide particles. Therefore, it can be seen that when calcium oxide nanoparticles are used according to the present invention, a composition for pest control with high dispersion stability can be prepared.

From the above description, those skilled in the art will understand that the present invention can be implemented in other specific forms without changing its technical idea or essential features. Therefore, the embodiments described above should be understood in all respects as illustrative and not restrictive. The scope of the present invention should be construed as including the meaning and scope of the patent claims described below rather than the detailed description above, and all changes or modified forms derived from the equivalent concept thereof are included in the scope of the present invention.

Claims (17)

Pathogenic mold or its spores;
silicone oil; and
Calcium oxide nanoparticles
A composition for controlling pests, comprising:
A composition wherein the calcium oxide nanoparticles have a particle diameter of 20 to 70 nm. The composition for controlling pests according to claim 1, which has improved storage stability above 50°C. The composition for controlling pests according to claim 1, wherein the pathogenic mold or its spores maintain a bacterial count of 1.0E+05 CFU/mL or more at room temperature and 54°C for at least 1 week. The method of claim 1, wherein the pathogenic mold or spores thereof are of the genus Beauveria , Isaria , Lecanicillium , Nomuraea , and Celletotrichum . Genus, Fusarium genus, Phytophthora genus, Sclerotinia , Metarhizium genus, Paecilomyces genus, Verticillium genus, Trichoderma Pest control, which is a fungus or spore thereof selected from the genus Trichoderma , Hirsutella , Aspergillus , Aschersonia , Metarrhizium , and combinations thereof. Composition for. The composition for controlling pests according to claim 1, wherein the pathogenic mold or spores thereof are Beauveria bassiana or spores thereof. The composition for controlling pests according to claim 1, wherein the pathogenic mold or its spores are contained in an amount of 1 to 90% by weight based on the total weight of the composition. The composition for controlling pests according to claim 1, wherein the calcium oxide nanoparticles are contained in an amount of 1 to 50% by weight based on the total weight of the composition. The composition for controlling pests according to claim 1, wherein the silicone oil has a viscosity of 0.1 to 1000 cP at 25°C. The composition for controlling pests according to claim 1, wherein the silicone oil is contained in an amount of 30 to 95% by weight based on the total weight of the composition. The composition for controlling pests according to claim 1, wherein the composition further comprises a surfactant. The composition for controlling pests according to claim 10, wherein the surfactant has an HLB value of 1 to 20. The composition for controlling pests according to claim 10, wherein the surfactant is contained in an amount of 0.001 to 4% by weight based on the total weight of the composition. The method of claim 10, wherein the surfactant is one selected from the group consisting of lauryl PEG/PPG-18/18 methicone, cetyl diglyceryl tris(trimethylsiloxy)silylethyl dimethicone, and PEG-9 dimethicone. The composition for controlling pests as described above. The composition for controlling pests according to claim 1, wherein the composition is in the form of a concentrate for spraying. A pest control method comprising the step of applying the pest control composition of any one of claims 1 to 14 to pests, their habitats, or crops susceptible to pests. Pathogenic mold or its spores;
silicone oil; and
A method for stabilizing the pest control composition of any one of claims 1 to 14, comprising the step of mixing and storing calcium oxide nanoparticles,
The method wherein the calcium oxide nanoparticles have a particle size of 20 to 70 nm. delete