KR20210001787A - Stable composition for controlling pests comprising nanoparticles of calcium oxide and method for controlling pests by using the same - Google Patents

Abstract

The present invention relates to a composition for controlling pests having excellent stability against high temperature or moisture, and to a method for controlling pests using the same and, more specifically, to a composition for controlling pests, comprising calcium oxide nanoparticles and having an oil-based formulation in order to solve a stability problem during storage of the composition for controlling pests.

Description

Stable composition for controlling pests comprising nanoparticles of calcium oxide and method for controlling pests by using the same}

The present invention relates to a composition for controlling pests having excellent stability against high temperature or moisture, and a method for controlling pests using the same.

In order to control pests and pests that damage agricultural crops, the use of chemical pesticides has increased further and the spraying area has become wider as the scale of cultivated crops increases with the development of agricultural technology. The emergence of organic synthetic pesticides after World War II marked a remarkable turning point in two aspects: the increase of agricultural crops through the control of agricultural pests and the improvement of human health through the control of sanitary pests. The amount of pesticides used for pest control in Korea is increasing every year, of which organophosphorus and carbamates account for about 90% of the total consumption.

However, due to decades of continuous use and abuse of these existing organic synthetic pesticides, various side effects such as emergence of resistant pests, human animal toxicity, biological control disturbance by natural enemies, residual poisons and environmental pollution have been caused. The use of synthetic pesticides is gradually being restricted as interest in the side effects and environmental impacts of the drug increase. Accordingly, biological control is being actively studied as a part of the development of next-generation pesticides that can replace synthetic pesticides, but biological control is an obstacle to commercialization because it is not easy to handle using microorganisms, natural enemies, or natural extracts.

이상) 노출 시 1주일 내 모두 사멸하게 되는 단점이 있다. 작물보호제의 유통과정 중 40

이상의 온도에 노출되는 경우는 흔히 발생하는 일이다.In this regard, microbial crop protection agents that biologically control insect pathogenic fungi (fungi) have the advantage of minimizing resistance and environmental pollution by using fungal spores, unlike the chemical pesticides described above, but it is an important matter in commercialization. Studies on mass production and stabilized formulation are still insufficient. In particular, in order to control pests such as thrips, which absorb nutrients by absorbing the juice of plant leaves and reduce the value of feed, insect pathogenic fungi ( Beauveria bassiana ) are currently sold in granular form. However, formulations using these insect pathogenic fungi should be prepared by separating the spores produced through solid culture from the culture and mixing them with water in the form of a liquid formulation and a wettable powder for spray application. However, when mixed with water, spores are 1 It dies within a week and is somewhat stable at room temperature in powder form, but at high temperature (50

Above) There is a disadvantage that all of them die within a week when exposed. In the process of distribution of crop protection agents 40

It is common to be exposed to temperatures above.

Due to this problem, conventional insect pathogenic fungal preparations have many problems in physical properties and are inconvenient to use, and the expiration date is 1 year, so it is difficult to export and sell to other countries, so if storage stability is improved, a large ripple effect is expected.

In this regard, in Korean Patent Publication No. 10-2016-0084968, the strain of Boberia bassiana JEF007, an insect pathogenic fungus having an insect pathogenicity and excellent sporulation ability, and a liquid formulation for controlling agricultural pests using the same, and the strain using a filterable bag It discloses a method of manufacturing a liquid formulation containing, but does not mention a matter on storage stability.

Therefore, there is an urgent need for the development of a new pest control formulation that is harmless to the human body, is environmentally friendly, is easy to handle, and has excellent storage stability against high temperature and moisture.

Korean Patent Publication No. 10-2016-0084968 (2016.07.15)

Under this background, the present inventors have repeatedly studied to solve the stability problem that pathogenic fungi spores are inhibited by various factors such as moisture or high temperature. As a result, when combining silicon oil and calcium oxide nanoparticles with pathogenic fungi raw agents, By remarkably reducing the problem of reducing the stability of pathogenic fungi formulations due to high temperature or moisture, it was confirmed that a composition for controlling pests and a method for controlling pests having excellent stability can be provided, thereby completing the present invention.

For example, there is provided a composition for controlling pests, including pathogenic fungi or spores thereof, silicon oil and calcium oxide nanoparticles.

In another example, a pest control method is provided, comprising the step of applying the composition for controlling pests to pests, habitats of pests, or crops susceptible to pests.

As another example, there is provided a method for stabilizing a composition for controlling pests comprising the step of mixing and storing pathogenic fungi or spores thereof, silicon oil and calcium oxide nanoparticles.

In one aspect, the present invention relates to a composition for controlling pests, including pathogenic fungi or spores thereof, silicon oil and calcium oxide nanoparticles as an oily solvent.

As used herein, "pathogenic fungi" refers to fungi that cause disease in organisms and mainly proliferate through spores. The "entomopathogenic fungi", which is a preferred example of the pathogenic fungi to which the present invention is applicable, mainly belongs to imperfect fungi and conjugate fungi, taxonomically, and refers to fungi having pest control activity. Insect pathogenic fungi are different from insect pathogenic bacteria or viruses that are infected through host feeding, after fungal spores adhere to the outer shell of host insects and germinate, then pass through the cuticle layer or germinated from the spores with an invasion tool. It directly passes through the cuticle layer and invades the inside of the insect. At this time, the physical action and enzyme actions such as chitinase, protease, lipase, and esterase occur together. Fungi that invade the insect's body can be used as insect quarantine insecticides because they secrete toxic substances while proliferating in the insect's body to block the insect's immune function and kill host insects.

More than 700 species of insect pathogenic fungi are currently reported, and any one having an insecticidal effect on insects by generating spores can be used without limitation. For example, the beam Beria (Beauveria) in director Liao (Isaria), A Greca nisil Solarium (Lecanicillium) in ah (Nomuraea), A celecoxib Sat tree glutamicum (Celletotrichum) genus Fusarium (Fusarium) to Nomura in blood Saratov Tora (Phytophthora), A's Klee Loti California (Sclerotinia), Metairie Clear (Metarhizium), A par indeed My process (Paecilomyces), A tying yarn Solarium (Verticillium) genus Trichoderma (Trichoderma), A heosyu telra (Hirsutella ), Aspergillus , Aschersonia , or The fungi of the genus Metarhizium can be exemplified. Specifically, the correction Beria Bridge Ana (Beauveria bassiana), Metairie Clear No Cattle Plymouth Oh (Metarhizium anisopliae), Bertie Room Solarium Recaro you (Verticillium lecanii), ASCII Sonia Alessio This display (Aschersonia aleyrodis) rilreyiyi or to Nomura (Nomuraea rileyi ) Can be illustrated. The boberia bassiana used in an example of the present application is a strain having excellent insecticidal effect against saw-legged ants, yellow flower thrips, and the like.

Such pathogenic fungi or spores thereof may be appropriately included in an amount capable of exhibiting a pest control effect, but preferably may be included in an amount of 1 to 90% by weight, and more specifically 1 to 30, based on the total weight of the composition for controlling pests. It may be included in wt%, more specifically 5 to 20 wt%, more specifically 7 to 15 wt%, most specifically 8 to 10 wt%, but is not limited thereto.

Spores are germ cells produced by fungi for asexual reproduction, and can develop into adults without forming a zygote, and preferably include conidia. The composition for controlling pests of the present application includes insect pathogenic fungi or spores thereof, which is understood to include spores, hyphae, mycelium, fruiting bodies, spores or their equivalents. When the spores touch the insect's shell, a germination tube is created from the spore, and the germination tube grows until the nutrients in the spore are depleted, and begins to penetrate the cuticle layer of the insect, and the germination tube penetrates the enzyme that dissolves the cuticle layer. Secrete.

In a specific embodiment, as the original agent of the composition for controlling pests according to the present invention, Boberia bassiana ERL836 was used, but the present invention is not limited thereto.

이다. 공기 중에 방치하면 수분과 이산화탄소를 흡수하여 수산화칼슘(소석회)과 탄산칼슘으로 분해한다. 또한, 물을 작용시키면 발열(發熱)하여 수산화칼슘이 되는 물질이다. 이러한 칼슘 옥사이드는 석회석 또는 탄산칼슘 CaCO₃을 약 900

이상으로 가열하면 생성된다. 이러한 칼슘 옥사이드 나노입자는 제형 내의 수분량을 안정성을 최대화할 수 있는 수준, 예를 들어 1 중량% 이하의 수준으로 조절할 수 있고, 보다 구체적으로는 0.5 중량% 이하, 0.3 중량% 이하, 가장 구체적으로는 0.1 중량% 이하 수준으로 조절할 수 있지만, 이에 한정되는 것은 아니다.In the present invention, in particular, in order to solve the stability problem due to high temperature or moisture, as an additive, it is characterized by including calcium oxide (CaO) nanoparticles having a particle diameter of 70 nm or less. The "calcium oxide" is also called quicklime, and the chemical formula is CaO. Pure is a white crystal of equiaxed crystal system, melting point 2,570

to be. When left in the air, it absorbs moisture and carbon dioxide and decomposes into calcium hydroxide (slaked lime) and calcium carbonate. In addition, it is a substance that becomes calcium hydroxide by heating when water is applied. These calcium oxides contain about 900 limestone or calcium carbonate CaCO ₃ .

It is produced when heated above. These calcium oxide nanoparticles can adjust the amount of moisture in the formulation to a level that can maximize stability, for example, 1% by weight or less, more specifically 0.5% by weight or less, 0.3% by weight or less, most specifically It can be adjusted to a level of 0.1% by weight or less, but is not limited thereto.

In one embodiment, the particle diameter of the calcium oxide nanoparticles may be 70 nm or less, for example, 20 to 70 nm, 30 to 70 nm, 40 to 70 nm, or 50 to 70 nm, but is not limited thereto. Calcium oxide nanoparticles having such particle diameters have a slower precipitation rate than calcium oxide microparticles having a general size, so that dispersion can be maintained for a long time. As a result of comparing the degree of precipitation of calcium oxide particles by the height ratio over time using calcium oxide nanoparticles and microparticles in the same composition as in the examples of the present invention, the nanoparticles maintain dispersion for about 1000 times longer until they are precipitated. It was found to be (Fig. 2).

In addition, the calcium oxide nanoparticles may be included in 1 to 50% by weight, more specifically 1 to 30% by weight, 3 to 20% by weight, or 4 to 10% by weight, based on the total weight of the pest control composition However, it is not limited thereto.

Further, the composition for controlling pests according to the present invention is characterized in that it contains silicone oil as an oily solvent. Silicone oil has little change in viscosity over a wide temperature range, has excellent heat resistance and cold resistance, is colorless, transparent, nonvolatile, and harmless to the human body, so it maintains the stability of the pest control composition better than other oily solvents. Make it possible. Preferably, the content of moisture in the silicone oil is characterized in that 0.1% or less.

In addition, with respect to the total weight of the composition for controlling pests, the silicone oil may contain 30 to 95% by weight, more specifically 60 to 95% by weight, 60 to 90% by weight, 70 to 90% by weight, or 80 to 87 It may be a weight percent, but is not limited thereto. Further, the silicone oil may be selected to have an appropriate viscosity as needed, but preferably may have a viscosity of 0.1 to 1000 cP at a temperature of 25°C, more specifically 0.5 to 500 cP, most specifically May represent a viscosity of 0.5 to 200 cP, but is not limited thereto. In a specific example, in the composition for controlling pests according to the present invention, KF96-100cs (100 cP viscosity), which is a dimethyl silicone oil, was used as a silicone oil.

The composition for controlling pests of the present invention may also contain a surfactant. Surfactants are amphiphilic substances having both a hydrophilic molecular group and a lipophilic molecular group in a molecule, and have cleaning power, dispersing power, emulsifying power, solubilizing power, wetting power, sterilizing power, foaming power and/or penetrating power, and for controlling pests according to the present invention. Pathogenic fungi or spores in the composition may act to hydrate, suspend or disperse to effectively exert a pest control effect.

Surfactants include, by way of non-limiting examples, hydrophilic surfactants, lipophilic surfactants, or mixtures thereof. For example, the hydrophilic lipophilic balance (HLB) value of the surfactant may be about 1 to 20, about 1 to 17, or 2 to 12. In one embodiment, the HLB of the surfactant may be 1 to 7, or 10 to 17, or 10 to 20. In another embodiment, the HLB of the surfactant may be 1 or more to less than 7, 10 to less than 17, or 10 to less than 20. The HLB range exemplifies a suitable range in which a surfactant can effectively dissolve in an oily solvent and exhibit a surfactant effect, but the scope of the present invention is not limited thereto. HLB is an index indicating the balance between hydrophilicity and lipophilicity (hydrophobicity) of a surfactant, and it is understood that the greater the HLB, the greater the proportion of hydrophilicity, and the smaller the HLB, the greater the proportion of lipophilic (hydrophobicity). The surfactant may also be nonionic, anionic, cationic, or zwitterionic (or amphoteric).

Examples of cationic surfactants that can be used herein include DTAB (Dodecyltrimethylammonium bromide) or CTAB (Cetyltrimethylammonium bromide), and examples of anionic surfactants include DSS (dioctyl sulfosuccinate sodium), SDS (sodium dodecyl sulfate), LDS (lithium dodecyl sulfate), sodium dodecyl benzene sulfonate (SDBS), sodium dodecylsulfonate (SDSA), and the like. Examples of amphoteric surfactants include lecithin, and examples of nonionic surfactants include La. Uryl PEG/PPG-18/18 Methicone, Cetyl Diglyceryl Tris(Trimethylsiloxy)silylethyl Dimethicone, PEG-9 Dimethicone (PEG-9 dimethicone), PEG-10 dimethicone, Sorbitan monooleate, Octylphenol Ethoxylate, polyethylene glycol, alkyl polyglucoside, silane poly Alkylene oxide (copolymer), nonylphenol ethoxylate, tristyrylphenol ethoxylate, polyoxyethylene alkyl ether, polyoxyethylene alkylphenyl ether, alkylallyl formaldehyde condensed polyoxyethylene ether, polyoxyethylene poly Oxypropylene block polymer, polyoxyethylene polyoxypropylene alkyl ether, polyoxyethylene ether of glycerin ester, polyoxyethylene ether of sorbitan ester, polyoxyethylene ether of sorbitol ester, polyethylene glycol fatty acid ester, glycerin ester, polyglycerin ester , Sorbitan ester, propylene glycol ester, sucrose ester, aliphatic Acid alkanol amide, polyoxyethylene fatty acid amide, alcohol ethoxylate, amine ethoxylate, glucoside, glucamide, polyethylene glycol, poly(ethylene glycol-co-propylene glycol), cetyl alcohol, stearyl alcohol, cetoste Aryl alcohol, oleyl alcohol, octaethylene glycol monododecyl ether, pentaethylene glycol monododecyl ether, polyoxypropylene glycol alkyl ether, decyl glucoside, lauryl glucoside, octyl glucoside, polyoxyethylene glycol octylphenol ether, nonoxy Nol-9, glycerol alkyl ester, glyceryl laurate, polyoxyethylene glycol sorbitan alkyl ester, polysorbate, sorbitan alkyl ester, span, cocamide MEA, cocamide DEA, dodecyldimethylamine oxide , A block copolymer of polyethylene glycol, polypropylene glycol, and a silane polyalkylene oxide (copolymer), or polyoxyethylene alkyl amide, for example, Triton (registered trademark) X-114, X-102, X-100, X-45, X-15, BG-10 or CG-119, or BRIJ (registered trademark) polyoxyethylene-4-lauryl ether (brij30), polyoxyethylene-23-lauryl ether (brij35 ), polyoxyethylene-2-cetyl ether (brij52), polyoxyethylene-10-cetyl ether (brij56), polyoxyethylene-20-cetyl ether (brij58), polyoxyethylene-2-stearyl ether (brij72) , Polyoxyethylene-10-stearyl ether (brij76), polyoxyethylene-2-oleyl ether (brij93) or polyoxyethylene-10-oleyl ether (brij97), PEG400 dioleate (PEG-8 dioleate) Such as may be illustrated, but is not limited thereto.

In a preferred embodiment, the surfactant is at least one selected from the group consisting of lauryl PEG/PPG-18/18 methicone, cetyl diglyceryl tris(trimethylsiloxy)silylethyl dimethicone, and PEG-9 dimethicone. However, it is not limited thereto. In a specific embodiment of the present application, a formulation containing Dow 5200 (lauryl PEG/PPG-18/18 methicone) was prepared, but the scope of the present invention is not limited thereto.

In a preferred embodiment, the surfactant may contain 0.001 to 90% by weight, more specifically 0.001 to 50% by weight, 0.005 to 20% by weight, 0.005 to 4% by weight, 0.01 based on the total weight of the pest control composition. It may be to 4% by weight, 0.01 to 2% by weight, or 0.05 to 1.0% by weight, but is not limited thereto. However, if it is less than the above range, there may be a problem that the surfactant effect does not appear sufficiently, and if it exceeds the above range, there may be a problem that the surfactant is not completely dissolved in the solvent and precipitation or precipitation occurs.

The composition for controlling pests according to the present invention may be prepared according to a known method for preparing a composition for controlling pests. For example, it can be prepared by combining pathogenic fungi or spores thereof, silicone oil and calcium oxide nanoparticles, and optionally a surfactant. Pathogenic fungi or spores thereof can be pulverized to have an appropriate particle size if necessary, and after preparing a liquid sample by adding a surfactant and calcium oxide nanoparticles to an oily solvent, the pulverized pathogenic fungi or spores thereof are mixed. The composition for controlling pests according to the invention can be prepared. The composition for controlling pests according to the present invention may be formulated in various forms, but a liquid concentrate form capable of spraying is preferred.

The composition for controlling pests according to the present invention exhibits excellent stability at high temperatures and moisture. Specifically, the composition for controlling pests according to the present invention is at 50°C, or 54°C or higher, for example, at 50°C to 60°C, 50°C to 55°C, or 50°C to 54°C, the insect pathogenic fungus or More than 1.0% of its spores can survive for over a week. In addition, at room temperature, or 50°C, or 54°C or more, for example 50°C to 60°C, 50°C to 55°C, or 50°C to 54°C, at least one week, preferably at least 5 weeks It is characterized in that to maintain. In addition, at room temperature, or at 50° C., or 54° C. or higher, for example, at 50° C. to 60° C., 50° C. to 55° C., or 50° C. to 54° C., preferably at least the number of effective bacteria for 5 weeks, specifically It is characterized by maintaining the number of bacteria of 1.0E+05 CFU/mL or more.

In a specific embodiment, after uniformly pulverizing the spores, KF96-100cs, which is a silicon oil, and calcium oxide nanoparticles are separately mixed, dispersed through ball-milling, and then Dow 5200 is used as a surfactant. After introduction into the system, vortexing was performed for 30 seconds and then sterilized, and then vortexed for 1 minute after adding the original agent to prepare a composition for controlling pests according to the present invention. It was confirmed that it exhibits excellent storage stability for 5 weeks (see Fig. 1).

In another aspect, the present invention comprises a silicone oil as an oily solvent and calcium oxide nanoparticles as an additive in a composition for controlling pests comprising a pathogenic fungus or spores thereof and a surfactant as a dispersing agent, so that insect pathogenic fungi or spores thereof It relates to a method of increasing the survival rate of. Preferably, the silicon oil and calcium oxide nanoparticles maintain the insect pathogenic fungus or its spores for a long period of time in a state where the usable moisture content is sufficiently low to increase the survival rate of the insect pathogenic fungus or its spores compared to a state where the moisture content is high. It is characterized by increasing.

In another aspect, the present invention relates to a method for stabilizing a composition for controlling pests, comprising the step of mixing and storing pathogenic fungi or spores thereof, silicon oil and calcium oxide nanoparticles.

In another aspect, the present invention relates to a method for controlling pests by applying the composition for controlling pests according to the present invention to pests, habitats of pests, or crops susceptible to pests.

The composition of the present invention may be used in a method for controlling pests by applying the composition for controlling pests according to the present invention to pests, habitats of pests, or crops susceptible to pests.

Examples of pests controllable by the composition of this embodiment include arthropods such as insects and ticks, especially harmful arthropods such as harmful insects and harmful mites, and the following can be exemplified:

- half bandwagon (Hemiptera): myeolguryu (Delphacidae), for example aemyeolgu (Laodelphax striatellus), Brown Planthopper (Nilaparvata lugens) and huindeung myeolgu (Sogatella furcifera); Cicadas (Deltocephalidae), such as Nephotettix cincticeps and Nephotettix virescens ; Jinditmulryu (Aphididae), for example, cotton aphid (Aphis gossypii), Green Peach Aphid (Myzus persicae), cabbage powder aphid (Brevicoryne brassicae), potato beard aphid (Macrosiphum euphorbiae), bush clover beard aphid (Aulacorthum solani), millet border aphid (Rhopalosiphum padi ) and tangerine aphid ( Toxoptera citricidus ); Pentatomidae, such as Nezara antennata , Saw-legged ant, Riptortus clavetus , Leptocorisa chinensis , Eysarcoris parvus , Halyomorpha mista and Halyomorpha mista ( Lyuslineolaris ); Aleyrodidae , such as Trialeurodes vaporariorum , Bemisiatabaci , and Bemisia argentifolii ; Snapworms ( Coccidae ), such as California red snapworm ( Aonidiella aurantii ), San Jose snapworm ( Comstockaspis perniciosa ), arrowhead bug ( Unaspiscitri ), ruby snapworm ( Ceroplastes rubens ), and iseria snapworm ( Icerya purchasi ) ; Shield bugs (Tingidae); Psyllidae , etc.;

-Lepidoptera : Pyralidae , such as Chilo suppressalis , Yellow rice beetle ( Tryporyzaincertulas ), Cnaphalocrocrocis medinalis , Cotton moth ( Notarchaderogata ), Hwaranggok moth ( Plodia interpunctella ) Moth ( Ostrinia furnacalis ), European lighted moth ( Ostrinia nubilaris ), Chinese cabbage sprout moth ( Hellula undalis ) and grass insect moth ( Pediasiateterrellus ); Chestnut moths ( Noctuidae ), such as tobacco moth ( Spodoptera litura ), green chestnut moth ( Spodoptera exigua ), Pseudaletia separata , thief moth ( Mamestra brassicae ), roundup seminar ( Agrotis ipsilon ), trapezoidal chestnut Butterfly ( Plusianigrisigna ), Thoricoplusia spp., tobacco chestnut moth ( Heliothis spp.) and tobacco moth ( Helicoverpa spp.); White butterfly ( Pieridae ), such as Chinese cabbage white butterfly ( Pierisrapae ); Tortricidae , for example, Adoxophyes spp., Peach moth ( Grapholitamolesta ), Bean moth ( Leguminivora glycinivorella ), Red bean moth ( Matsumuraeses azukivora ), Apple apex moth ( Adoxophyesorana fasciata ) pattern ipmalyi moth (Adoxophyes honmai), tea leaves, horse moth (Homona magnanima), geommo pattern ipmalyi moth (Archipsfuscocupreanus) and code Lin moth (Cydia pomonella); Fine moths ( Gracillariidae ), such as Camellia theivora ( Caloptilia theivora ) and Apple oyster moth ( Phyllonorycter ringoniella ); Heart food moth ( Carposinidae ), such as peach heart food moth ( Carposina niponensis ); Oyster moth ( Lyonetiidae ), such as silver pattern oyster moth ( Lyonetia spp.); Poisonous moths ( Lymantriidae ), such as gypsy moths ( Lymantria spp.) and poisonous moths ( Euproctis spp.); House moth ( Yponomeutidae ), such as Chinese cabbage moth ( Plutella xylostella ); Geelechiidae , such as Pectinophora gossypiella and Phthorimaea operculella ; Fire moth ( Arctiidae ), such as American white fire moth ( Hyphantria cunea ); And grain moths ( Tineidae ), such as Tinea translucens ;

-Thysanoptera: yellow flower thrips ( Frankliniella occidentalis ), cucumber thrips ( Thrips palmi ), convex thrips ( Scirtothrips dorsalis ), thrips ( Thripstabaci ), Taiwanese thrips ( Frankliniella intonsa ) and tobacco thrips Worms ( Frankliniella fusca ), etc.;

-Diptera: oyster flies ( Agromyzidae ), such as oyster flies ( Hylemyaantiqua ), sedge flies ( Hylemyaplatura ), rice leaf oyster flies ( Agromyza oryzae ), rice leaf oyster flies ( Hydrellia griseola ), rice yellow oyster flies ( Chlorops oryzae ) , American leaf oyster fly ( Liriomyza trifolii ); Melon fly ( Dacus cucurbitae ) and Mediterranean fruit fly ( Ceratitis capitata ), etc.;

- second bandwagon (Coleoptera): 28-spotted ladybird (Epilachna vigintioctopunctata), cucumber beetle (Aulacophora femoralis), flea beetle (Phyllotreta striolata), rice beetle (Oulema oryzae), rice root weevil (Echinocnemus squameus), byeomul weevil (Lissorhoptrus oryzophilus ), Cotton Weevil ( Anthonomus grandis ), Red Weevil ( Callosobruchus chinensis ), Hunting Weevil ( Sphenophorus venatus ), Japanese Bean Beetle ( Popillia japonica ), Copper Beetle ( Anomala cuprea ), Corn Leaf Beetle ( Diabrotica spp.), Colorado Potato Leaf Beetle ( . Leptinotarsa decemlineata), skipjack acids (Agriotes spp), and the like cigarette beetle (Lasioderma serricorne);

-Grasshoppers (Orthoptera): land dog ( Gryllotalpa africana ), rice grasshopper ( Oxya yezoensis ) and rice grasshopper ( Oxya japonica ), etc.;

-Hymenoptera : leafless bee ( Athalia rosae ), scissor ants (worker ants) ( Acromyrmex spp.) and fire ants ( Solenopsis spp.), etc.;

-Mites (Acarina): mites (Tetranychidae), such as spotted mites ( Tetranychus urticae ), tangerine mites ( Panonychus citri ) and tree mites ( Oligonychus spp.); Hawke Mites (Eriophyidae), for example gyulnok mite (Aculops pelekassi); Dust mites ( Tarsonemidae ), such as tea dust mites ( Polyphagotarsonemus latus ); Earth mite ( Tenuipalpidae ); Chire Mites (tuckerellidae); Powdery mites ( Acaridae ), such as long hairy mites ( Tyrophagus putrescentiae ); Dust mites ( Pyroglyphidae ), such as large leg dust mites ( Dermatophagoides farinae ) and vertical pattern dust mites ( Dermatophagoides ptrenyssnus ); Claw mites ( Cheyletidae ), such as short claw mites ( Cheyletus eruditus ), claw mites ( Cheyletus malaccensis ) and Keyletus moorei ( Cheyletus moorei );

-Nematodes : rice leaf nematodes ( Aphelenchoides besseyi ) and strawberry sub-nematodes ( Nothotylenchusacris ), etc.

One example of a method for controlling pests using the composition of the present application is to spray the composition of the present application on a pest or pest habitat of a treatment target. "Pest habitat" means the area where the pest actually lives, as well as at least the area where the pest passes or moves. In addition, it means an area in which a habitat or passage of a pest is assumed, as well as an area in which the pest is preferably eliminated.

Examples of plants to which the compositions or methods herein can be applied are as follows:

-Crops: corn, rice, wheat, barley, rye, oats, sorghum, cotton, soybean, peanut, buckwheat, sugar beet, rapeseed, sunflower, sugar cane and tobacco;

-Vegetables: Eggplant and vegetables (eggplant, tomato, bell pepper, pepper, potato, etc.), gourd and vegetables (cucumber, pumpkin, zucchini, watermelon, melon, etc.), cruciferous vegetables (wagered radish, turnip, horseradish, kohlrabi, cabbage, etc.) Cabbage, black mustard, broccoli, cauliflower, rapeseed, etc.), asteraceae vegetables (burdock, garland chrysanthemum, artichoke, lettuce, etc.), lilies and vegetables (green onion, onion, garlic, asparagus, etc.), parsley (carrot, parsley, celery, etc.) , Parsnip, etc.), pollock vegetables (spinach, chard, etc.), lamiaceae vegetables (perilla, mint, basil, etc.), strawberries, sweet potatoes, yams, taro, etc.;

-Fruit trees: causal fruits (apple, pear, Japanese pear, Chinese quince, quince, etc.), stone fruits (peach, plum, nectarine, loquat, cherry, apricot, prune, etc.), citrus fruits (Wenzhou mandarin, orange, lemon, lime, etc.) Grapefruit), nuts (chestnuts, walnuts, hazelnuts, almonds, pistachios, cashews, macadamia nuts, etc.), berries (blueberries, cranberries, blackberries, raspberries, etc.), grapes, persimmons, olives, loquats, bananas, coffee, dates , Coconut, oil palm, etc.;

-Trees other than fruit trees: green tea, mulberry, flower tree (azalea, camellia, hydrangea, baby camellia, Japanese star anise, cherry tree, tulip tree, lagerstroemia tree, gypsy tree, etc.), street trees (ash, birch Tree, Dogwood, Eucalyptus, Ginkgo, Lilac, Maple, Oak, Poplar, Bacterial Tree, Taiwan Style Tree, Plane Tree, Zelkova, Japanese Cypress, Fir, Hem-Fir, Juniper, Pine, Spruce, Yew Tree , spruce, elm, Aesculus turbinata, etc.), sanhosu (coral tree), or Hansong (podocarpus), cedar, cypress, croton (croton), Euonymus japonicus (Euonymusjaponicus), Photinia glabra (Photiniaglabra), etc .;

-Lawn : Grass (zoysiagrass, Zoysiamatrella , etc.), umbrella grass ( Cynodondactylon, etc.), top (Bent grass) ( Agrostisalba ), creeping bent grass, highland bent grass, hiland bent), bluegrass (meadow grass, bird grass, etc.), fescue (tall fescue), chewings fescue, creeping red fescue red fescue, etc.), ryegrass (darnel, ryegrass, etc.), orchard grass, timothy grass, etc.;

-Others: flowers (rose, carnation, chrysanthemum, prairie gentian), gypsophila, gerbera, marigold, salvia, petunia, verbena, tulip , Aster, gentian, lily, pansy, cyclamen, orchid, lily of the valley (convallaria), lavender, stock, ornamental cabbage, primula, poinsettia, gladiolus ), cattleya, daisy, cymbidium, begonia, etc.), biofuel plants (Jatropha, safflower, camelina, switchgrass, silver grass ( Miscanthus), reed canary grass, giant reed, kenaf, cassava, willow, etc.), ornamental plants, etc.

The pest control composition of the present invention may be applied to plants or plant cultivation areas for pest control. Plants as used herein include stems or leaves of plants, flowers of plants, fruits of plants, seeds of plants, and the like. Examples of application methods include application to stems and leaves of plants, such as foliage application; Application to the seeds of plants; And applications to areas where plants are cultivated, such as soil applications and submerged applications.

The pest control composition according to the present invention uses silicon oil as an oily solvent and calcium oxide nanoparticles as an additive, and excellently improves the stability problem caused by high temperature and moisture, which is a problem in the conventional pest control composition, for pest control When the composition is stored, it is very effective by reducing the decrease in titer due to temperature and moisture.

1 is a graph showing the spore concentration when treated for 5 weeks at room temperature and 54° C. storage conditions in order to confirm the stability of Examples and Comparative Examples according to the present invention.
2 is a graph showing the precipitation degree of calcium oxide nanoparticles and general calcium oxide microparticles used in the present invention as a height ratio over time.

Hereinafter, the present invention will be described in detail through Examples and Experimental Examples, but the following Examples and Experimental Examples are only illustrative of the present invention, and the scope of the present invention is not construed as being limited to the following Examples and Experimental Examples. .

Preparation of ingredients

As the pathogenic spore strain, Beauveria bassiana ERL836 (KCCM11506P) was used. For the cultivation of the fungus, solid culture was performed using grain as a medium material. For this, first, spores of Boberia bassiana ERL836 stored at -70°C were spread on potato agar medium (Potato Dextrose Agar), and then cultured at 25°C for 7 days. Spores were recovered, inoculated into millet, and cultured for 1 week to perform seed culture. For this culture, millet was immersed in water to sufficiently contain moisture, and then put in a steamer and steamed for 10 minutes or more at a high temperature of 100°C or higher. The increased millet was cooled at room temperature, and the seed culture was inoculated and mixed. The mixture was incubated at 25° C. for 5 days or longer, and the culture was dried at low temperature to recover spores. When the number of spores in the original formulation was 1 X 10 ⁹ CFU/g or more, it was taken as normal culture.

Silicone oil (KF96-100cs) was used as an oily solvent, Dow 5200 (lauryl PEG/PPG-18/18 methicone, nonionic, HLB=6.8) was used as a surfactant, and calcium oxide with a particle size of 50-70 nm Nanoparticles were self-synthesized and used.

Experimental Example 1: Preparation and storage stability analysis of the composition for controlling pathogenic pests according to the present invention

A composition for controlling pathogenic pests was prepared according to the following method of the composition for controlling pathogenic pests comprising silicon oil and calcium oxide nanoparticles according to the present invention.

Test Methods:

i) Boberia bassiana ERL836, the original pathogenic spore, was uniformly crushed with a mortar.

ii) Samples of the original agent, oil, surfactant, and calcium oxide nanoparticles according to the conditions of Table 1 were prepared.

Unit: mass ratio (wt%) sample Original title Silicone oil Surfactant (Dow 5200) Calcium oxide nanoparticles Example 1 9.1 85 1.4 4.5 Comparative Example 1 100 0 0 0

-Original title: ERL836 (Beauveria bassiana)-Oil: Silicone oil (KF96-100cs)

-Surfactant: Dow 5200 (lauryl PEG/PPG-18/18 methicone)

-Calcium oxide nanoparticles: Calcium oxide of 50-70nm particle size

iii) Samples prepared according to the content ratio in Table 1 were prepared as follows.

-Example 1: Oil and calcium oxide nanoparticles were mixed and dispersed through ball-milling, a surfactant was added, and vortexing was performed for 30 seconds. Thereafter, sterilization was performed at 80° C. for 30 minutes, and the original agent was added and vortexing was added for 1 minute to prepare a composition according to the example.

-Comparative Example 1: A 100% by weight sample of the original agent was prepared.

iv) Each test sample was stored at room temperature (25°C) and 54°C, respectively.

v) Each stored sample sample was taken at 1 week intervals for a total of 6 weeks and 1 ml of sample samples were taken at 1 week intervals, diluted in aqueous solution (NaCl 0.85 wt% and dioctyl sulfosuccinate sodium 0.27 wt%), After vortexing for 1 minute was added and dispersed, it was spread on PDA (Tartaric acid) medium and cultured in an incubator at 25°C.

vi) The number of active spore colonies was counted (unit: CFU (Colony forming unit)/mL) through viable spore count.

보관 조건에서 포자가 관찰되지 않은 것에 비해, 실시예 1의 해충 방제용 조성물은 칼슘 옥사이드 나노입자를 포함함으로 인해 54

보관 조건에서 5주차까지 약효가 유효한 1.0E+05 cfu/mL 이상의 보관 안정성을 나타내어 현저하게 안정성이 향상된 것임을 확인할 수 있었다. The test results are shown in FIG. 1. As can be seen in Figure 1, Comparative Example 1 using the original agent itself is 54

Compared to the fact that no spores were observed in the storage conditions, the composition for controlling pests of Example 1 contained calcium oxide nanoparticles.

In storage conditions, it was confirmed that the stability was remarkably improved by exhibiting storage stability of 1.0E+05 cfu/mL or more, which is effective for the drug until the 5th week.

Experimental Example 2: Comparison of dispersibility according to calcium oxide particle size

The composition of Example 1 prepared in Experimental Example 1 and the composition of Comparative Example 2 having the same composition as Example 1 but containing calcium oxide microparticles having a particle diameter of 3 to 5 μm instead of calcium oxide nanoparticles were left at room temperature. The degree of precipitation of calcium oxide particles over time was observed, and this was expressed as a height ratio over time.

As a result, as shown in Figure 2, the composition of Example 1 of the present invention, compared to the composition of Comparative Example 2, the precipitation rate of calcium oxide particles was slow, it was found that the dispersion was maintained for about 1000 times longer. Therefore, it can be seen that, when using calcium oxide nanoparticles according to the present invention, a composition for controlling pests having high dispersion stability can be prepared.

From the above description, those of ordinary skill in the art to which the present invention pertains will understand that the present invention can be implemented in other specific forms without changing the technical spirit or essential features thereof. Therefore, the embodiments described above are illustrative in all respects and should be understood as non-limiting. The scope of the present invention should be construed that all changes or modifications derived from the meaning and scope of the claims to be described later rather than the above detailed description, and equivalent concepts thereof, are included in the scope of the present invention.

Claims (17)

Pathogenic fungi or spores thereof;
Silicone oil; And
Calcium oxide nanoparticles
Containing, pest control composition. The composition for controlling pests according to claim 1, wherein storage stability is improved at 50° C. or higher. The composition for controlling pests according to claim 1, wherein the pathogenic fungi or spores thereof maintain a number of bacteria of 1.0E+05 CFU/mL or more for 1 week or more at room temperature and 54°C. The method of claim 1, wherein the pathogenic fungi or spores thereof are the genus Beauveria , the genus Isaria , the genus Lecanicillium , the genus Nomuraea , and the celletotrichum . genus Fusarium (Fusarium) in blood Saratov Tora (Phytophthora), A's Klee Loti California (Sclerotinia), Metairie Clear (Metarhizium), A par indeed My process (Paecilomyces), A tying yarn Solarium (Verticillium) genus Trichoderma ( Trichoderma ) genus, Hersutella ( Hirsutella ) genus, Aspergillus ( Aspergillus ) genus, Aschersonia ( Aschersonia ) genus , Metarhizium (Metarrhizium ) a fungus selected from the genus (Metarrhizium ) genus and combinations thereof, pest control Dragon composition. The composition for controlling pests according to claim 1, wherein the pathogenic fungi or spores thereof are Boberia bassiana or spores thereof. The composition for controlling pests according to claim 1, wherein the pathogenic fungi or spores thereof are contained in an amount of 1 to 90% by weight based on the total weight of the composition. The composition for controlling pests according to claim 1, wherein the calcium oxide nanoparticles have a particle diameter of 20 to 70 nm. The composition for controlling pests according to claim 1, wherein the calcium oxide nanoparticles are contained in an amount of 1 to 50% by weight based on the total weight of the composition. The composition for controlling pests according to claim 1, wherein the viscosity of the silicone oil is 0.1 to 1000 cP at 25°C. The composition for controlling pests of claim 1, wherein the silicone oil is contained in an amount of 30 to 95% by weight based on the total weight of the composition. The composition for controlling pests according to claim 1, wherein the composition further comprises a surfactant. The composition for controlling pests according to claim 11, wherein the surfactant has an HLB value of 1 to 20. The composition for controlling pests according to claim 11, wherein the surfactant is contained in an amount of 0.001 to 4% by weight based on the total weight of the composition. The method of claim 11, wherein the surfactant is one selected from the group consisting of lauryl PEG/PPG-18/18 methicone, cetyl diglyceryl tris(trimethylsiloxy)silylethyl dimethicone, and PEG-9 dimethicone. That is, the pest control composition. The composition for controlling pests according to claim 1, wherein the composition is in the form of a concentrate for spraying. A method for controlling pests, comprising the step of applying the composition for controlling pests of any one of claims 1 to 15 to pests, habitats of pests, or crops susceptible to pests. Pathogenic fungi or spores thereof;
Silicone oil; And
A method for stabilizing the composition for controlling pests of any one of claims 1 to 15, comprising the step of mixing and storing calcium oxide nanoparticles.

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