KR102648506B1 - Cosmetic composition containing mangrove root and guaajava leaf - Google Patents

Abstract

The present invention relates to a cosmetic composition containing mangrove root and guava leaf extract, and more specifically, to prepare a composition with excellent collagen synthesis promoting, antioxidant and anti-inflammatory effects from mangrove root extract and guava leaf, which can be used as a cosmetic raw material. It relates to a cosmetic composition containing mangrove root and guava leaf extract.
According to the present invention, a composition with excellent collagen synthesis promotion, antioxidant and anti-inflammatory effects can be extracted and used from mangrove root extract and guava leaves.

Description

Cosmetic composition containing mangrove root and guava leaf extract {COSMETIC COMPOSITION CONTAINING MANGROVE ROOT AND GUAAJAVA LEAF}

The present invention relates to a cosmetic composition containing mangrove root and guava leaf extract, and more specifically, to prepare a composition with excellent collagen synthesis promoting, antioxidant and anti-inflammatory effects from mangrove root extract and guava leaf, which can be used as a cosmetic raw material. It relates to a cosmetic composition containing mangrove root and guava leaf extract.

Skin phenomena that occur due to various internal and external stresses such as ultraviolet rays are changes in skin thickness, decreased cell count and skin metabolism, loss of bio-bound water, and destruction of collagen and elastin, resulting in a wrinkled and rough skin surface. In order to suppress skin aging, in addition to promoting collagen synthesis and anti-inflammatory effects, an antioxidant effect is also essential. Here, the antioxidant effect refers to dissolved oxygen dissolved in the air or water of fats and oils used as softeners in cosmetics. It means preventing oxidation and decomposition by active radicals induced by ultraviolet rays. Representative substances used as antioxidants in cosmetics include dibutylhydroxyanisole, dibutylhydroxytoluene, ascorbic acid and its derivatives, and tocopherol and its derivatives. However, dibutylhydroxyanisole and dibutylhydroxytoluene have excellent antioxidant effects but are known to cause skin side effects, and ascorbic acid and its derivatives and tocopherol are difficult to use directly due to their poor stability. The improved vitamin C and vitamin E derivatives have the problem of having little antioxidant effect.

The inflammatory response is characterized by pain, fever, seizures, swelling, and loss of function due to external stimuli. Histologically, it is characterized by expansion of arterioles, capillaries, and venules with increased permeability and blood flow, and plasma containing plasma proteins. Complex symptoms, including exudation and migration of white blood cells to the inflamed area, are observed, and anti-inflammatory refers to a series of actions that prevent the destruction of tissues caused by the above phenomenon from external stimuli. Recent research data has shown that coumarin has an anti-inflammatory effect by reducing the activity of phagocytes and lowering citrulline production.

mangrove root extract

It has excellent antioxidant effects in suppressing inflammation and scavenging free radicals, and cosmetic compositions containing it can be used as cosmetics for anti-aging purposes, preventing hair loss, and promoting hair growth.

Water extract of mangrove stems has antibacterial activity and is known to be effective in recovery after surgery. The effectiveness of mangroves is due to the activity of tannins contained in large quantities in the bark, and the bark of trees of the Rhizophora genus is known to contain phenolic compounds containing about 10-36% of tannin.

Guava (Psidium guajava L) leaf extract

Guava leaves contain a large amount of tannin, and have anti-diarrheal, allergy-inhibiting, blood sugar-lowering, anti-aging, anti-cancer, and vascular permeability inhibition effects, as well as allergic properties such as allergies, rhinitis, allergic dermatitis, hay fever, and bronchial asthma. It is known to be effective in treating diseases, eczema, colds, acute or chronic effects, peptic ulcers, dysentery, dyspeptic diarrhea, fatigue recovery, hypertensive skin, and whitening.

Tannin, also called tannic acid, is an amorphous substance that is light yellow to light brown in the form of powder, flakes, or spongy lumps. Distributed in plants, the tannin solution is acidic and has an astringent taste. It has hemostatic and astringent effects, so it has been used to treat tonsillitis, sore throat, hemorrhoids, and skin rashes. It is also taken to stop diarrhea and intestinal bleeding. It combines with toxic substances contained in metals, alkaloids, and glycosides to form insoluble precipitates. It was used as an antidote.

KR 10-2017-0068015 A

The present invention to solve the above-mentioned problems is to provide a cosmetic composition containing mangrove root and guava leaf extract, which allows extracting and using a composition with excellent collagen synthesis promoting, antioxidant and anti-inflammatory effects from the mangrove root extract and guava leaf. The purpose.

The present invention, devised to solve the above-mentioned problems, is an anti-aging cosmetic composition containing mangrove root and guava leaf extracts, comprising: 0.01 to 10% by weight of a mixed extract containing mangrove root and guava leaf; 0.1 to 10% by weight of oxygen water; It consists of 80 to 99.89% by weight of cosmetics.

The mixed extract is characterized in that it is extracted using at least one solvent selected from the group consisting of water, ethanol, glycerin, butylene glycol, and propylene glycol, or a mixed solvent with oxygenated water as a solvent.

The oxygen water is characterized in that it contains 20 to 200 ppm of oxygen.

The mixed extract is characterized in that the active ingredients are extracted by mixing the solvents and then heating at a temperature of 50 to 95 ° C. for 4 to 20 hours, or by soaking the extract for 1 to 15 days at a temperature of 5 to 37 ° C.

The cosmetic composition is a formulation selected from the group consisting of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing agents, oils, powder foundations, emulsion foundations, wax foundations and sprays. It is characterized by having a.

According to the present invention, a composition with excellent antioxidant and anti-inflammatory effects can be extracted and used from mangrove root extract and guava leaves.

Hereinafter, a "cosmetic composition containing mangrove root and guava leaf extract" (hereinafter referred to as 'cosmetic composition') according to an embodiment of the present invention will be described with reference to the drawings.

As a result of research to more effectively solve the above problems, it was discovered that a cosmetic composition containing mangrove root extract and guava leaf extract is safe and has excellent effects on the skin in terms of antioxidant and anti-inflammatory properties, making it a cosmetic composition with excellent skin safety and efficacy.

The present invention will be described in more detail as follows. After completely drying the mangrove roots and guava leaves, 1-20 times the volume of one or more solvents selected from the group consisting of water, ethanol, glycerin, butylene glycol, and propylene glycol or a mixed solvent with oxygen water as an extraction solvent relative to the dry weight. apply.

Extraction methods include heating at 50-95 ℃ for 4-20 hours with a cooling condenser to prevent evaporation of the solvent, or soaking at 5-37 ℃ for 1-15 days to extract the active ingredient. You can. The mixed extract obtained by mixing mangrove roots and guava leaves in this way is completely concentrated under reduced pressure while recovering the evaporated solvent using a distillation device equipped with a cooling condenser, and then 0.01 to 10% by weight of the dry weight, preferably 0.01 to 5%. It is added to cosmetics in an amount of % by weight. In other words, it is added to softening lotion (skin), nourishing lotion (lotion), nourishing cream, massage cream, essence, pack, emulsified foundation, etc.

The cosmetic composition consists of a mixed extract, oxygen water, and cosmetics, and contains 0.1 to 10% by weight of oxygen water and 80 to 99.89% by weight of cosmetics. In particular, the oxygen water preferably has an oxygen content of 20 to 200 PPM.

The following examples and experimental examples illustrate the content of the present invention, but the content of the present invention is not limited thereto.

Example

To confirm the antioxidant and anti-inflammatory effects of the mixed extract of mangrove roots and guava leaves, the mixed extract was prepared using the following solvent. In Examples 1 to 18, the extract was prepared without drying, and in Examples 19 to 22, the mixed extract was dried and prepared in powder form. In Examples 1 to 9 and 19 to 22, a method of depositing the mixture in an extractor was applied, and in Examples 9 to 18, a mixed extract was prepared by heating the mixture in an extractor.

In the examples of the present invention, 500 g each of mangrove roots and guava leaves were used, for a total of 1 kg.

Examples 1 to 9

In Examples 1 to 9, 1 kg of the dry mixture of mangrove roots and guava leaves was washed with purified water, then added to 6 kg of the solvent shown in Table 1 below, extracted by soaking at room temperature for 5 days with an extractor, filtered through a 400 mesh filter cloth, and 0.45 A liquid mixed extract was obtained by filtration through a ㎛ filter.

Examples 10-18

1kg of the dry mixture of mangrove roots and guava leaves was added to 6kg of the solvent shown in Table 2 below, heated at a temperature of 80°C for 3 hours with a cooling condenser to prevent the solvent from evaporating, and then filtered through a 400 mesh filter cloth. Afterwards, it was filtered through a 0.45 ㎛ filter to obtain a liquid mixed extract.

Examples 19-22

1kg of the dried mixture of mangrove roots and guava leaves washed with purified water was added to 6kg of the solvent shown in Table 3 below, extracted by immersing it in an extractor at room temperature for 6 days, filtered through a 400 mesh filter cloth, and then filtered through a 0.45 ㎛ filter. The filtered solution was dried under reduced pressure using a distillation device equipped with a cooling condenser to obtain a mixed extract in powder form.

Antioxidant effect experiment

Put 20 ㎕ of mixed extract of mangrove roots and guava leaves of each concentration in a glass bottle, mix 0.25 ㎖ of tween 60 (40% W/V) with 0.5 ㎖ of linoleic acid first, and then add 50 ㎖ of oxygenated water and emulsify for about 5 minutes. and neutralized using 25 ㎕ of 1 N KOH, then redispersed by adding 110 ㎖ of 0.05 M potassium phosphate buffer (pH 7.0). After adding 10 ㎖ (the final volume is about 160 ㎖), it was placed in a shaking incubator. ) was reacted at 180 rpm and 37°C for 1 hour. Next, 20 ㎕ of 0,0375 M FeSO ₄ ·7H ₂ O was added for peroxidation. After addition, it was reacted in a shaking incubator at 180 rpm and 37°C for 2 hours. 400 ㎕ of linoleic acid solution was placed in an E-tube and cold soaked at 4°C for 10 minutes. TBA reagent (43 mg of TBA) was dissolved in 10 ml of DW, and 2 ml of 6 M phosphoric acid solution was added thereto. It was mixed immediately before use. ) was added and mixed, and color was developed in boiling water for 15 minutes. It was cooled in ice water, and 560 ㎕ of n-BuOH was added and mixed. Centrifugation was performed at 3000 rpm for 20 minutes, and the absorbance of the BuOH (supernatant) portion was measured at UV-Spectrum 535 nm. The measurement results are shown in Table 4.

Among them, the antioxidant effect of the mixed extract prepared in Example 16 was measured separately at each concentration and is shown in Table 5.

Anti-inflammatory effect experiment

To 1 ml of 1 mM linoleic acid, 0.05 ml of mixed extract of mangrove roots and guava leaves, and 0.95 ml of 11,060 units/ml lipoxygenase dissolved in 0.1 M sodium phosphate buffer were added, mixed well, and reacted in a water bath at 25°C for 15 minutes. The reaction was terminated by adding 0.5 ml of 20% (w/v) trichloroacetic acid, 1 ml of 0.6% (w/v) TBA was added, and color was developed in boiling water for 15 minutes. Afterwards, it was immersed in ice water for 2 minutes, 2 ml of n-BuOH was added, mixed by shaking, and then centrifuged at 3,500 rpm for 5 minutes.

The upper layer (n-BuOH layer) was taken and the absorbance was measured at 535 nm in the UV-Visible spectrum to evaluate the anti-inflammatory effect (%). For the control standard, a solvent was used instead of the mixed extract. For the blank, 0.1 M sodium phosphate buffer was used instead of lipoxygenase. The measurement results are shown in Table 6.

The anti-inflammatory effect of the mixed extract prepared in Example 16 was measured separately at each concentration and is shown in Table 7.

Skin irritation test (closed patch test)

This mangrove root and guava leaf mixed extract (Example 16) 10%, 30% aqueous solution and 0.08% sodium lauryl sulfate aqueous solution were administered to 20 healthy adult men and women, and a certain amount (0.2 g) of each sample was applied to the back area for 24 hours. After applying the patch for a while, the pin chamber was removed, and after 4 hours, the change in skin condition was read with the naked eye, and the results are shown in Table 8.

** Skin irritation test results **

※ Criteria - ; No erythema or unusual symptoms

+- ; Slightly redder than the surrounding area

+ ; Significantly redder than surroundings

++ ; Severe redness and swelling compared to the surrounding area

Primary skin irritation test of powder samples (closed patch test)

0.5 g of the powdered extract was dissolved in 100 g of purified water and conducted in the same manner as Experiment 5, and the results are shown in Table 9.

It was manufactured in the same manner as the prescription example below using the raw materials used in the above examples.

Prescription example 1

Prescription examples of lotion (skin lotion) among cosmetics containing mixed extracts of mangrove roots and guava leaves are as follows. Here, the mixed extract of mangrove roots and guava leaves is that of Example 16.

〈Manufacturing method〉

Formulation Example 1: Add No. 2, 3, 4, and 8 in order to No. 11 and stir to dissolve. After dissolving No. 5 by heating to about 60°C, add No. 10, stir, and add to No. 11. Lastly, add Nos. 1, 6, 7, and 9, stir sufficiently, and then age.

Comparative Formulation Example 1: Add No. 2, 3, 4, and 8 in order to No. 11 and stir to dissolve. After dissolving No. 5 by heating to about 60°C, add No. 10, stir, and add to No. 11. Lastly, add Nos. 6, 7, and 9, stir thoroughly, and then age.

Prescription example 2

Examples of nutritional lotion formulations among cosmetics containing mixed extracts of mangrove roots and guava leaves are as follows. Here, the mixed extract of mangrove roots and guava leaves is that of Example 16.

〈Manufacturing method〉

Formulation Example 2: Raw materials 2, 3, 4, 5, and 6 are homogenized at a constant temperature and are referred to as nonionic amphiphilic lipids. The nonionic amphiphilic lipid and raw materials 1, 7, 8, and 14 are mixed and homogenized at a constant temperature, and then the raw material 9 is slowly added at a constant temperature and homogenized. Afterwards, 10, 11, 12, and 13 are added and dispersed to stabilize and mature.

Comparative Formulation Example 2: Raw materials 2, 3, 4, 5, and 6 were homogenized at a constant temperature and were referred to as nonionic amphiphilic lipids. The nonionic amphiphilic lipid and raw materials 7, 8, and 14 are mixed and homogenized at a constant temperature, and then the raw material 9 is slowly added at a constant temperature and homogenized. Afterwards, 10, 11, 12, and 13 are added and dispersed to stabilize and mature.

Prescription example 3

An example of a nutritional cream formulation among cosmetics containing mixed extracts of mangrove roots and guava leaves is as follows. Here, the mangrove root and guava leaf mixed extract is from Example 16.

〈Manufacturing method〉

Formulation Example 3: Raw materials 2, 3, 4, 5, and 6 are homogenized at a constant temperature and are referred to as nonionic amphiphilic lipids. The nonionic amphiphilic lipid and raw materials 1, 7, 8, and 14 are mixed and homogenized at a constant temperature. Then, raw material 9 is slowly added at a constant temperature to homogenize it, and then 10, 11, 12, and 13 are added and dispersed to stabilize and mature.

Comparative Formulation Example 3: Raw materials 2, 3, 4, 5, and 6 were homogenized at a constant temperature and were referred to as nonionic amphiphilic lipids. The nonionic amphiphilic lipid and raw materials 7, 8, and 14 are mixed and homogenized at a constant temperature. Then, raw material 9 is slowly added at a constant temperature to homogenize it, and then 10, 11, 12, and 13 are added and dispersed to stabilize and mature.

Test example 1

An experiment on the skin irritation effect of Formulation Examples 1 to 3 and Comparative Formulation Examples 1 to 3 in Prescription Examples 1 to 3 was measured as follows. Formulation and sodium lauryl sulfate 0.08% aqueous solution were applied to the back of 20 healthy adult men and women. A certain amount (0.2 g) of each sample was applied for 24 hours. The pin chamber was removed, and after 4 hours, the skin condition was examined with the naked eye. The changes were read and the results are shown in Table 10.

** Skin irritation test results **

※ Criteria - ; No erythema or unusual symptoms

+- ; Slightly redder than the surrounding area

+ ; Significantly redder than surroundings

++ ; Severe redness and swelling compared to the surrounding area

Although the preferred embodiments of the present invention have been described with reference to the accompanying drawings, the technical configuration of the present invention described above can be changed to other specific forms by those skilled in the art without changing the technical idea or essential features of the present invention. You will be able to understand that this can be implemented. Therefore, the embodiments described above should be understood in all respects as illustrative and not restrictive, and the scope of the present invention is indicated by the claims described later rather than the detailed description above, and the meaning and scope of the claims and All changes or modified forms derived from the equivalent concept should be construed as falling within the scope of the present invention.

Claims (5)

A cosmetic composition containing mangrove root and guava leaf extract,
0.01 to 10% by weight of a mixed extract containing mangrove roots and guava leaves;
0.1 to 10% by weight of oxygen water;
A cosmetic composition containing mangrove root and guava leaf extract, consisting of 80 to 99.89% by weight of cosmetic. According to paragraph 1,
The mixed extract is characterized in that it is extracted using either one or more solvents selected from the group consisting of water, ethanol, glycerin, butylene glycol, and propylene glycol, or a mixed solvent of the one or more solvents and oxygen water as a solvent, A cosmetic composition containing extracts of mangrove roots and guava leaves. According to paragraph 1,
A cosmetic composition containing mangrove root and guava leaf extract, wherein the oxygen water contains 20 to 200 ppm of oxygen. According to paragraph 2,
The mixed extract is characterized in that the active ingredient is extracted by mixing the solvents and then heating at a temperature of 50 to 95 ° C. for 4 to 20 hours, or soaking the extract for 1 to 15 days at a temperature of 5 to 37 ° C. A cosmetic composition containing extracts of mangrove roots and guava leaves. According to paragraph 1,
The cosmetic composition is a formulation selected from the group consisting of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing agents, oils, powder foundations, emulsion foundations, wax foundations and sprays. A cosmetic composition containing mangrove root and guava leaf extract, characterized in that it has.