KR102630197B1 - Cosmetic composition comprising hibiscus extract - Google Patents

Abstract

The present invention relates to a cosmetic composition containing a hibiscus functional extract, comprising: a first fermented extract obtained by fermenting a first mixture containing hibiscus flowers and stevia leaves with lactic acid bacteria; and/or a cosmetic composition comprising a hibiscus functional extract including a second fermented extract obtained by fermenting a second mixture containing plant collagen derived from hibiscus flowers and stevia with lactic acid bacteria.

Description

Cosmetic composition containing hibiscus functional extract {COSMETIC COMPOSITION COMPRISING HIBISCUS EXTRACT}

The present invention relates to a cosmetic composition containing a hibiscus functional extract, and more specifically, to a cosmetic composition containing a hibiscus functional extract that can express beneficial functions in the human body.

Hibiscus is a plant belonging to the dicotyledonous plant family Malvaceae and Rose of Sharon and has red petals. 'Anthocyanin', the ingredient that gives hibiscus its red color, is a powerful antioxidant and is known to help smooth metabolism and excretion of waste products. In addition, hibiscus contains various active ingredients that are beneficial to the human body, so it is used in cosmetics, food and beverages, and foods. It is widely used as a main ingredient. In particular, hydroxycitric acid (HCA) contained in hibiscus is a substance that reduces fat activity and has been recognized by the Ministry of Food and Drug Safety as a functional diet ingredient, and is receiving attention as a raw material for inner beauty foods and health functional foods not only among the young but also among the middle-aged. there is.

Recently popular inner beauty foods are collagen products, and hibiscus is included in various products. The molecular weight and molecular size (Dalton, Da) of commonly used collagen products are rather large for absorption into the body, so many companies are conducting a lot of research to increase the absorption of collagen molecules. Methods are used to increase absorption by physically reducing the size of collagen extracted or synthesized from raw materials, or by using cofactors that help collagen absorption, such as vitamin C. Hibiscus also contains various active ingredients and is used as an ingredient to increase collagen absorption.

As hibiscus has gained attention as an active ingredient in cosmetic compositions and foods, a process has been developed to extract the active ingredients of hibiscus at high concentration, and a high-performance hibiscus functional extract that can perform various functions such as antioxidant function and collagen synthesis in the body has been developed. Development is needed. Furthermore, it is necessary to develop highly efficient hibiscus functional extracts that can increase the absorption rate of collagen such as animal collagen, fish collagen, and vegetable collagen, and promote collagen synthesis in the body.

Korean Patent Publication No. 10-2020-0090628 Korean Patent No. 10-2299846

The present invention seeks to provide a hibiscus functional extract with a high content of active ingredients and a method for producing a hibiscus functional extract with excellent extraction of active ingredients from hibiscus.

In addition, the present invention seeks to provide a hibiscus functional extract that enhances the synthesis and absorption of collagen and a method for producing the same.

In addition, the present invention seeks to provide a hibiscus functional extract with excellent antioxidant function, elasticity and wrinkle improvement function, and a method for producing the same.

In order to solve the above problem, the present invention includes the steps of ① mixing hibiscus flowers and stevia to form a first mixture; ② It provides a method for producing a hibiscus functional extract, including the step of fermenting the first mixture using lactic acid bacteria to form a first fermented extract.

The above ① includes i) heating hibiscus flowers and stevia to 40 to 100°C and then drying them; ii) drying and then pulverizing; iii) pulverizing and then extracting to form a first mixture; providing a method for producing a hibiscus functional extract, characterized in that it consists of:

In addition, the present invention includes the steps of ③ mixing hibiscus flower-derived vegetable collagen and stevia to form a second mixture; ④ It provides a method for producing a hibiscus functional extract, further comprising: forming a second fermented extract after fermenting the second mixture using lactic acid bacteria.

The present invention provides a first fermented extract obtained by fermenting a first mixture containing hibiscus flowers and stevia with lactic acid bacteria; And/or provides a hibiscus functional extract comprising a second fermented extract obtained by fermenting a second mixture containing plant collagen derived from hibiscus flowers and stevia with lactic acid bacteria.

Additionally, the present invention provides a cosmetic containing the hibiscus functional extract.

Additionally, the present invention provides a food containing the hibiscus functional extract.

Foods containing the hibiscus functional extract include mixed lactose, fish collagen, glucose, fructose, citric acid, rosehip extract powder, silicon dioxide, enzyme-treated stevia, nicotinic acid amide, tart cherry, hyaluronic acid, elastin, aloe gel, vitamin B, Provides a health functional food further containing one or more selected from the group consisting of vitamin C and vitamin D.

The hibiscus functional extract according to the present invention has excellent antioxidant properties and is effective in promoting collagen synthesis.

In addition, the hibiscus functional extract according to the present invention has the effect of enhancing the absorption of collagen, so when mixed with collagen in a cosmetic composition, it has the effect of ensuring that the collagen component is well absorbed, and when mixed with collagen in food, the collagen component is absorbed. It is well absorbed into the body and has the effect of helping it to be well synthesized in the body.

In addition, the hibiscus functional extract according to the present invention contains hibiscus-derived vegetable collagen, has a better absorption rate than animal collagen and fish collagen, and has effects such as improving skin elasticity, alleviating wrinkles, and preventing aging.

Below, embodiments of the present invention will be described in detail so that those skilled in the art can easily implement it. However, the present invention may be implemented in many different forms and is not limited to the embodiments described herein.

The terms or words used in the specification and claims of the present invention are not to be construed as limited to their ordinary or dictionary meanings, and the inventor may appropriately define the concept of terms to explain his or her invention in the best way. It must be interpreted as meaning and concept consistent with the technical idea of the present invention based on principles.

Throughout the specification of the invention, when it is said that a part "includes" a certain component, this means that it may further include other components rather than excluding other components, unless specifically stated to the contrary.

Throughout the specification of the present invention, “A and/or B” means A or B, or A and B.

Although the present invention is described in detail below, the present invention is not limited thereto.

The present invention provides a hibiscus functional extract and a method for producing the same.

An example of the present invention includes ① mixing hibiscus flowers and stevia to form a first mixture; ② A method for producing a hibiscus functional extract, comprising: forming a first fermented extract after fermenting the first mixture using lactic acid bacteria.

Hibiscus flowers or petals are used to form the first mixture. Hibiscus flowers are red and contain a large amount of ingredients such as anthocyanin and protocatesilicic acid, which have excellent antioxidant activity. It is known that pyruvic acid in Hibiscus sabdarfa extract not only has a fat-decomposing and weight-loss effect when consumed with DHA (dihydroxyacetone), but also has a protein-dissolving effect. Additionally, when applied to the skin, it dissolves proteins to remove dead skin cells and increase skin regeneration rate.

Stevia leaves and stems contain stevioside, a component that gives a sweet taste, so when forming the first mixture, stevia leaves or stems can be used. For example, stevia leaves can be used. In addition, an extract obtained by extracting the stevioside component from stevia leaves or stems can be used, or enzyme-treated stevia that has gone through an enzyme treatment process to remove the bitter taste of stevia can be used. Additionally, without being limited thereto, various forms of stevia that can be used in cosmetic compositions or foods can be used. Stevia has excellent antioxidant activity and has effective functions such as improving immunity through antiviral activity, so it can exert excellent antioxidant effect along with hibiscus.

The first mixture is a mixture of hibiscus flowers and stevia, and may be a mixture of the raw materials themselves, an extract extracted after mixing the raw materials, or a mixture of extracts of the raw materials.

The extraction method is performed according to a conventional method, that is, under conventional conditions of temperature and pressure, using a conventional solvent (e.g., water, anhydrous or hydrous lower alcohol having 1-4 carbon atoms (e.g., methanol, ethanol, It can be obtained using at least one extraction solvent selected from the group consisting of propanol and butanol), acetone, ethyl acetate, chloroform, butyl acetate, 1,3-butylene glycol, and mixtures thereof, preferably ethanol, 70 It is obtained using % (v/v) ethanol aqueous solution or water, and most preferably, it is obtained using 70% ethanol aqueous solution.

Fermentation of lactic acid bacteria can be carried out in a conventional manner, and the fermentation process can be carried out using a species of lactic acid bacteria that can generally be used as a starter.

For example, the fermentation strain may be one or more of Lactobacillus paracasei, Lactobacillus bifidus, Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus bulgaricus, Lactobacillus rhamnosus, Lactobacillus plantarum, Lactobacillus reuteri, Lactobacillus confusus, Lactobacillus fermentum, and Lactobacillus brevis, but is not limited thereto. Any fermentation strain used for plant fermentation can also be used.

When hibiscus flowers and stevia are fermented with lactic acid bacteria, the active ingredients of hibiscus flowers and stevia are extracted more easily than when they are not fermented, resulting in better antioxidant function and the effect of further improving collagen absorption and collagen synthesis efficiency in the body. there is. In addition, the extraction of active ingredients from hibiscus may be more effective when fermented with lactic acid bacteria by mixing stevia with it, rather than when hibiscus flowers are fermented with lactic acid bacteria alone. The stevioside component of stevia or other components may interact with the active ingredients of hibiscus flowers, specifically collagen, to facilitate the extraction of active ingredients that assist in the absorption and synthesis of collagen.

Another example of the present invention includes the steps of ① i) heating hibiscus flowers and stevia to 40 to 100°C and then drying them; ii) forming a first mixture by drying and extracting; A method for producing hibiscus functional extract, characterized in that it consists of.

As an example, the hibiscus flowers and stevia leaves may be placed in water at a temperature of 40 to 80° C. for 2 to 4 hours, then dried or freeze-dried, and an extract may be formed according to a conventional extraction method.

As another example, the hibiscus flowers and stevia leaves may be harvested in a moisture-free state at a temperature of 60 to 100° C. and then dried or freeze-dried to form an extract according to a conventional extraction method. The roasting time may take 20 to 30 minutes, and may be adjusted depending on the condition of the raw materials (hibiscus flowers, stevia leaves).

When the heating step is performed, there is an effect of further increasing the extraction efficiency of the active ingredient in the extraction step compared to the case where the heating step is not performed, and there is an effect of further increasing the fermentation efficiency during the lactic acid bacteria fermentation process.

As mentioned above, the heating range can be a constant temperature (40 to 80°C) with moisture added, or a constant temperature (60 to 100°C) without moisture. Since the active ingredient may escape into the surrounding moisture when moisture is added, heating without adding moisture can relatively increase the extraction efficiency of the active ingredient in the next extraction step.

In i) above, hibiscus flowers and stevia may be used as raw materials, or may be used in the form of powder. In forming the first mixture, it is more preferable to use hibiscus flowers and stevia in powder form for extracting the active ingredients. The powder may be in the form of mechanically pulverized raw materials, or commercially available powder may be used.

As an example, the hibiscus flower powder may be in the form of pulverized hibiscus petal raw materials, dried and then pulverized, or freeze-dried and then pulverized. By expanding the cross-sectional area of hibiscus flowers as much as possible, the content of extracted active ingredients can be increased.

As an example, the stevia powder may be in the form of drying and pulverizing stevia leaves or stem raw materials, or may be in the form of freeze-drying and pulverizing the raw material. Alternatively, you can use commercially available stevia powder or enzyme-treated stevia powder. By expanding the cross-sectional area of stevia as much as possible, the content of extracted active ingredients can be increased.

The extraction can be carried out using a conventional extraction method as mentioned above.

Another example of the present invention includes the steps of ① i) heating hibiscus flowers and stevia to 40 to 100°C and then drying them; ii) drying and then pulverizing; iii) pulverizing and extracting to form a first mixture; A method for producing hibiscus functional extract, characterized in that it consists of.

As an example, the hibiscus flowers and stevia leaves may be placed in water at a temperature of 40 to 80° C. for 2 to 4 hours, then dried or freeze-dried, and pulverized to form an extract according to a conventional extraction method.

As an example, the hibiscus flowers and stevia leaves may be harvested in a moisture-free state at a temperature of 60 to 100° C., then dried or freeze-dried, and pulverized to form an extract according to a conventional extraction method. The roasting time may take 20 to 30 minutes, and may be adjusted depending on the condition of the raw materials (hibiscus flowers, stevia leaves).

When the heating step is performed, there is an effect of further increasing the extraction efficiency of the active ingredient in the extraction step compared to the case where the heating step is not performed, and there is an effect of further increasing the fermentation efficiency during the lactic acid bacteria fermentation process.

As mentioned above, the heating range can be a constant temperature (40 to 80°C) with moisture added, or a constant temperature (60 to 100°C) without moisture. Since the active ingredient may escape into the surrounding moisture when moisture is added, heating without adding moisture can relatively increase the extraction efficiency of the active ingredient in the next extraction step.

The grinding refers to physical grinding or mechanical grinding, and includes all general physical grinding including nano grinding.

The extraction can be carried out using a conventional extraction method as mentioned above.

In forming the first mixture, when hibiscus flowers and stevia are heated at a certain temperature and then extracted through a drying process, or when the drying process is followed by a grinding process, extraction of the active ingredients of the raw materials is compared to extraction without such process. This has an even better effect.

Another example of the present invention is ③ mixing hibiscus flower-derived vegetable collagen and stevia to form a second mixture; ④ A method for producing a hibiscus functional extract, further comprising: forming a second fermented extract after fermenting the second mixture using lactic acid bacteria.

Representative collagens include animal collagen and fish collagen, but plants also contain collagen, and hibiscus is one of the representative plants from which vegetable collagen can be extracted.

The vegetable collagen derived from hibiscus flowers may be a commercially available ingredient, or the collagen component may be obtained from hibiscus flowers using a known technique.

A known method of extracting hibiscus-derived vegetable collagen includes adding water to hibiscus, heat-treating it at high temperature, adding an acidic solution, and adding proteolytic enzyme to obtain collagen through a hydrolysis process. Another method is to obtain collagen through alcohol extraction and hot water extraction followed by separation and purification using a centrifuge.

Vegetable collagen derived from hibiscus flowers has a weight average molecular weight of 1,500 to 1,000 daltons (Da) or less, and has high skin penetration and absorption rates, showing excellent anti-aging, anti-wrinkle, and whitening activities.

The stevia used in forming the second mixture may be the same as the stevia mentioned in the first mixture.

The lactic acid bacteria used in forming the second fermented extract can be carried out in the same manner as the method mentioned in the first fermented extract.

By further including the hibiscus flower-derived vegetable collagen in the functional extract, there is an effect of further enhancing the synthesis and absorption of collagen.

In addition, as the plant collagen derived from hibiscus flowers is fermented with lactic acid bacteria, the active ingredient content is higher and the absorption rate is effective compared to the case without such process.

In addition, by mixing stevia during the fermentation process, there is an effect of increasing the fermentation rate and improving the extraction rate of active ingredients and absorption rate of active ingredients.

Compared to using the first fermented extract alone, when the second fermented extract is used in combination, the effect of improving elasticity and wrinkles is superior. In addition, compared to using the second fermentation extract alone, when the first fermentation extract is used in combination, the absorption of the second fermentation extract is improved, thereby improving collagen absorption and synthesis.

An example of the present invention is a first fermented extract obtained by fermenting a first mixture containing hibiscus flowers and stevia with lactic acid bacteria; and/or a hibiscus functional extract comprising a second fermentation extract obtained by fermenting a second mixture containing plant collagen derived from hibiscus flowers and stevia with lactic acid bacteria.

An example of the present invention is a cosmetic or cosmetic composition containing the hibiscus functional extract.

The cosmetic composition can be prepared in various forms, such as emulsions, lotions, creams (oil-in-water, water-in-oil, multi-phase), solutions, suspensions (anhydrous and aqueous), anhydrous products (oil and glycol-based), It can be manufactured in formulations such as gel, mask, pack, or powder. The cosmetic composition of the present invention may contain a carrier acceptable in cosmetic preparations in addition to its active ingredients. Here, “acceptable carrier in cosmetic preparations” refers to a compound or composition that is already known and used or a compound or composition to be developed in the future that can be included in a cosmetic preparation and has no toxicity beyond that to which the human body can adapt upon contact with the skin. The carrier may be included in the composition of the present invention in an amount of about 1 to 99.99% by weight based on the total weight.

An example of the present invention is a food, functional food, health functional food, inner beauty food, or food composition containing the hibiscus functional extract.

Examples of the food composition include hibiscus functional extract, mixed lactose, fish collagen, glucose, fructose, citric acid, rosehip extract powder, silicon dioxide, enzyme-treated stevia, nicotinic acid amide, tart cherry, hyaluronic acid, elastin, aloe gel, vitamins. It may contain two or more selected from the group consisting of B, vitamin C, and vitamin D.

The hibiscus functional extract according to the present invention assists the absorption of fish collagen and has the effect of increasing the absorption rate. Additionally, vitamin C also has a useful effect in helping the absorption of collagen.

An example of the hibiscus functional extract according to the present invention includes vegetable collagen derived from hibiscus flowers, and the vegetable collagen has a relatively excellent absorption rate compared to fish collagen, and in particular, fermented vegetable collagen derived from hibiscus flowers has a high absorption rate. Even better.

Hereinafter, the present invention will be described in detail by way of examples.

Example 1. Preparation of first fermentation extract

Example 1-1.

A sample of 100 g of hibiscus (Hibiscus sabdariffa) flowers and 20 g of stevia leaves was mixed to form a first mixture.

2 g of Lactobacillus rhamnosus lactic acid bacteria are activated in a solid medium at room temperature for 24 hours, then inoculated into a sterilized liquid medium and cultured with shaking at room temperature for 24 hours before being used as seed.

Lactobacillus rhamnosus lactic acid bacteria previously cultured were added to the liquid medium containing the first mixture, cultured at room temperature for 5 days and fermented, and then a first fermented extract was prepared.

Example 1-2.

100g of hibiscus (Hibiscus sabdariffa) flowers and 20g of stevia leaves are added to 1L of distilled water and heated at 50°C for 2 hours, then moisture is removed and freeze-dried. After drying and grinding with a machine, the pulverized product is added to 1L of distilled water, heated (60-80℃, 2 hours, repeated 3 times), and the extract obtained by hot water extraction is filtered through filter paper to form the first mixture.

2 g of Lactobacillus rhamnosus lactic acid bacteria are activated in a solid medium at room temperature for 24 hours, then inoculated into a sterilized liquid medium and cultured with shaking at room temperature for 24 hours before being used as seed.

Lactobacillus rhamnosus lactic acid bacteria previously cultured were added to the liquid medium containing the first mixture, cultured at room temperature for 5 days and fermented, and then a first fermented extract was prepared.

Example 1-3.

Roast 100g of hibiscus (Hibiscus sabdariffa) flowers and 20g of stevia leaves three times for 10 minutes at a temperature of 78℃ to remove moisture. The pulverized product obtained by freeze-drying and then grinding with a machine is added to 1L of distilled water, heated (60-80℃, 2 hours, repeated 3 times), and the extract obtained by hot water extraction is filtered through filter paper to form the first mixture.

2 g of Lactobacillus rhamnosus lactic acid bacteria are activated in a solid medium at room temperature for 24 hours, then inoculated into a sterilized liquid medium and cultured with shaking at room temperature for 24 hours before being used as seed.

Lactobacillus rhamnosus lactic acid bacteria previously cultured were added to the liquid medium containing the first mixture, cultured at room temperature for 5 days and fermented, and then a first fermented extract was prepared.

Example 2. Preparation of second fermentation extract

Prepare hibiscus flower-derived vegetable collagen extracted from hibiscus flowers. A second mixture is formed by mixing 10 g of vegetable collagen derived from hibiscus flowers and 1 g of enzyme-treated stevia.

2 g of Lactobacillus rhamnosus lactic acid bacteria are activated in a solid medium at room temperature for 24 hours, then inoculated into a sterilized liquid medium and cultured with shaking at room temperature for 24 hours before being used as seed.

Lactobacillus rhamnosus lactic acid bacteria previously cultured were added to the liquid medium containing the second mixture, cultured at room temperature for 5 days and fermented, and then a second fermented extract was prepared.

Example 3. Preparation of mixed fermentation extract of first and second fermentation extracts

Example 3-1.

The first fermentation extract of Example 1-1 and the second fermentation extract of Example 2 were mixed at a weight ratio of 10:1.

Example 3-2.

The first fermentation extract of Example 1-2 and the second fermentation extract of Example 2 were mixed at a weight ratio of 10:1.

Example 3-3.

The first fermentation extract of Example 1-3 and the second fermentation extract of Example 2 were mixed at a weight ratio of 10:1.

Example 4. Preparation of lotion

8 to 12 parts by weight of fermented extract of Example 1, Example 2 or Example 3, 2 to 5 parts by weight of glycerin, 1 to 3 parts by weight of butylene glycol, 1 to 3 parts by weight of propylene glycol, 0.1 to 0.1 parts by weight of carboxyvinyl polymer Toner containing 0.5 parts by weight, 0.1 to 0.5 parts by weight of PEG-12 nonylphenyl ether, 0.1 to 0.5 parts by weight of polysorbate, 8 to 12 parts by weight of ethanol, 0.1 to 0.5 parts by weight of triethanolamine, and 100 to 120 parts by weight of purified water. Prepared according to conventional methods.

Example 5. Inner beauty food production

4-5 parts by weight of fermented extract of Example 1, Example 2 or Example 3, 30-40 parts by weight of mixed lactose, 20-30 parts by weight of fish collagen, 20-30 parts by weight of glucose, 3-10 parts by weight of fructose , Vitamin C 1-3 parts by weight, natural raspberry flavor 1-3 parts by weight, citric acid 0.5-1 part by weight, rosehip extract powder 0.5-1 part by weight, silicon dioxide 0.5-1 part by weight, enzyme-treated stevia 0.5-1 part by weight , nicotinic acid amide (niacin) 0.01 to 0.1 parts by weight, vitamin B1 hydrochloride 0.01 to 0.1 parts by weight, vitamin B6 hydrochloride 0.01 to 0.1 parts by weight, Lactobacillus rhamnosus 0.01 to 0.1 parts by weight, Bifidobacterium longum 0.01 to 0.1 parts by weight , Bifidobacterium breve 0.01 to 0.1 parts by weight, Bifidobacterium lactis 0.01 to 0.1 parts by weight, Bifidobacterium Bifidum 0.01 to 0.1 parts by weight, Lactobacillus plantarum 0.01 to 0.1 parts by weight, aloe vera gel Powdered inner beauty food containing 0.01 to 0.1 parts by weight, 0.01 to 0.1 parts by weight of elastin peptide, and 0.01 to 0.1 parts by weight of vitamin B2 is prepared according to a conventional method.

Comparative Example 1. Preparation of first fermentation extract without stevia leaf addition

Prepare a sample of 100 g of hibiscus (Hibiscus sabdariffa) flowers.

2 g of Lactobacillus rhamnosus lactic acid bacteria are activated in a solid medium at room temperature for 24 hours, then inoculated into a sterilized liquid medium and cultured with shaking at room temperature for 24 hours before being used as seed.

Pre-cultured Lactobacillus rhamnosus lactic acid bacteria were added to the liquid medium containing the sample, cultured at room temperature for 5 days, fermented, and a fermented extract was prepared.

Comparative Example 2. Preparation of second fermentation extract without stevia leaf addition

Prepare hibiscus flower-derived vegetable collagen extracted from hibiscus flowers.

2 g of Lactobacillus rhamnosus lactic acid bacteria are activated in a solid medium at room temperature for 24 hours, then inoculated into a sterilized liquid medium and cultured with shaking at room temperature for 24 hours before being used as seed.

Pre-cultured Lactobacillus rhamnosus lactic acid bacteria were added to the liquid medium containing the sample, cultured at room temperature for 5 days, fermented, and a fermented extract was prepared.

Comparative Example 3. Preparation of vegetable collagen derived from hibiscus flowers

Prepare hibiscus flower-derived vegetable collagen extracted from hibiscus flowers.

Experimental Example 1. Comparison experiment measuring antioxidant activity

Antioxidant activity was measured using DPPH (2.2-diphenyl-1-picryl hydrezyl) radical scavenging activity. The DPPH solution dissolved in ethyl-alcohol and filtered was mixed 1:1 with the fermentation extract of Example 1 or 3, left in the dark at room temperature for 10 minutes, and the absorbance was measured at 515 nm using a Microplate reader (BioTek, USA). DPPH radical removal rate (%) was calculated. 0.1 mg/ml ascorbic acid was used as a positive control to remove radicals.

[DPPH radical removal rate measurement method]

DPPH radical scavenging rate (%) = (A - B) / A x 100

(A is the absorbance obtained from the blank test solution, B is the absorbance obtained from the test solution)

A total of 5 measurements were made, and the average values were rounded and shown in Table 1 below.

control group Example 1-1 Example 1-2 Example 1-3 Antioxidant power (%) 92.6 64.4 76.2 78.1

Example 3-1 Example 3-2 Example 3-3 Comparative Example 1 Antioxidant power (%) 62.6 74.1 76.2 49.8

Experimental Example 2. Comparison experiment of measuring collagen synthesis rate

Fibroblast cell lines were cultured in DMEM containing gentamicin and 10% FBS at 37°C in a 5% CO ₂ incubator. Fibroblast cells were inoculated into a well plate, cultured, and then irradiated with 12.5mJ of UV at a wavelength of 312nm. Afterwards, DMEM medium was treated with Examples 1, 2, and 3 and Comparative Examples 1, 2, and 3 and further cultured for 5 days. After the culture was completed, the medium was collected and used as a Procollagen Type-I C-Peptide (PIP) EIA kit. The amount of collagen produced was measured using (Takara Bio Inc. MK101). After measuring the absorbance at 450 nm using a microplate reader (BioTek, USA), a standard concentration curve was created to calculate the amount of collagen. The standard value for the amount of collagen before UV irradiation was set at 100. A total of 5 measurements were made, and the average value was rounded and shown.

Before UV irradiation After UV irradiation Example 1-1 Example 1-2 Example 1-3 Example 2 amount of collagen 100 43.9 62.2 67.3 68.1 60.8

Example 3-1 Example 3-2 Example 3-3 Comparative Example 1 Comparative Example 2 Comparative Example 3 amount of collagen 70.2 74.2 76.3 56.8 54.6 50.3

It can be confirmed that the examples according to the present invention show excellent collagen synthesis ability from stimulation by ultraviolet rays and have excellent anti-aging effect.

Experimental Example 3. Comparison experiment measuring MMP-1 activity inhibition by ultraviolet rays

Fibroblast cell lines were cultured in DMEM medium containing gentamicin (50ug/mL) and 10% FBS at 37°C in a 5% CO ₂ incubator. Fibroblast cells were inoculated into a well plate, cultured, and then irradiated with 12.5mJ of UV at a wavelength of 312nm. Afterwards, DMEM medium was treated with Examples 1, 2, and 3 and Comparative Examples 1, 2, and 3 and further cultured for 5 days. After the culture was completed, the medium was collected and used with an MMP-1 measurement kit (RPN 2610, Amersham Bioscience, Absorbance was measured at 450 nm using an ELISA reader (UK). The standard value for the amount of MMP-1 after UV irradiation was set at 100. A total of 5 measurements were made, and the average value was rounded and shown.

After UV irradiation Example 1-1 Example 1-2 Example 1-3 Example 2 MMP-1 amount 100 53.5 48.6 47.4 44.6

Example 3-1 Example 3-2 Example 3-3 Comparative Example 1 Comparative Example 2 Comparative Example 3 MMP-1 amount 48.7 43.9 42.8 60.7 50.6 56.8

It can be confirmed that the examples according to the present invention have excellent performance in suppressing the expression level of MMP-1, which inhibits collagen synthesis.

Claims (1)

Hibiscus functional extract including a first fermented extract obtained by fermenting a first mixture containing hibiscus flowers and stevia leaves with lactic acid bacteria, and a second fermented extract obtained by fermenting a second mixture containing plant collagen derived from hibiscus flowers and enzyme-treated stevia with lactic acid bacteria. 8 to 12 parts by weight, 2 to 5 parts by weight of glycerin, 1 to 3 parts by weight of butylene glycol, 1 to 3 parts by weight of propylene glycol, 0.1 to 0.5 parts by weight of carboxyvinyl polymer, 0.1 to 0.5 parts by weight of PEG-12 nonylphenyl ether. A cosmetic composition comprising 0.1 to 0.5 parts by weight of polysorbate, 8 to 12 parts by weight of ethanol, 0.1 to 0.5 parts by weight of triethanolamine, and 100 to 120 parts by weight of purified water.