KR102590861B1 - 세포주 개발을 위한 상동 재조합 벡터의 고속생성을 위한 dna 플라스미드 - Google Patents

세포주 개발을 위한 상동 재조합 벡터의 고속생성을 위한 dna 플라스미드 Download PDF

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KR102590861B1
KR102590861B1 KR1020197015851A KR20197015851A KR102590861B1 KR 102590861 B1 KR102590861 B1 KR 102590861B1 KR 1020197015851 A KR1020197015851 A KR 1020197015851A KR 20197015851 A KR20197015851 A KR 20197015851A KR 102590861 B1 KR102590861 B1 KR 102590861B1
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오스카 엠. 페레스-릴
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템플 유니버시티-오브 더 커먼웰쓰 시스템 오브 하이어 에듀케이션
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KR1020197015851A 2016-11-03 2017-11-03 세포주 개발을 위한 상동 재조합 벡터의 고속생성을 위한 dna 플라스미드 Active KR102590861B1 (ko)

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KR1020237035059A KR102736524B1 (ko) 2016-11-03 2017-11-03 세포주 개발을 위한 상동 재조합 벡터의 고속생성을 위한 dna 플라스미드

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Application Number Priority Date Filing Date Title
US201662416802P 2016-11-03 2016-11-03
US62/416,802 2016-11-03
PCT/US2017/059816 WO2018144087A2 (en) 2016-11-03 2017-11-03 Dna plasmids for the fast generation of homologous recombination vectors for cell line development

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KR102590861B1 true KR102590861B1 (ko) 2023-10-18

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KR1020197015851A Active KR102590861B1 (ko) 2016-11-03 2017-11-03 세포주 개발을 위한 상동 재조합 벡터의 고속생성을 위한 dna 플라스미드

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US (2) US12359222B2 (enExample)
EP (2) EP3535394B1 (enExample)
JP (1) JP7418796B2 (enExample)
KR (2) KR102736524B1 (enExample)
CN (1) CN110199020B (enExample)
CA (1) CA3081687A1 (enExample)
IL (1) IL266422A (enExample)
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Families Citing this family (6)

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JP7418796B2 (ja) 2016-11-03 2024-01-22 テンプル・ユニバーシティ-オブ・ザ・コモンウェルス・システム・オブ・ハイアー・エデュケイション 細胞株の開発のための相同組換えベクターの高速生成のためのdnaプラスミド
CN109402172B (zh) * 2018-11-23 2021-11-26 四川农业大学 一种鸭坦布苏报告病毒的制备方法及其产品和应用
CN113260700B (zh) * 2018-12-30 2024-01-30 豪夫迈·罗氏有限公司 基于可检测标签与靶蛋白的CRISPR/Cas控制的整合而选择细胞的方法
CN111235183B (zh) * 2020-01-13 2021-07-27 东南大学 一种talen表达载体及其快速制备方法及靶基因和细胞双标记系统和应用
CN116721700B (zh) * 2023-08-08 2024-01-12 中国人民解放军军事科学院军事医学研究院 鉴定新型双链dna胞苷脱氨酶的方法、装置及应用
WO2025106834A1 (en) * 2023-11-17 2025-05-22 Temple University-Of The Commonwealth System Of Higher Education A single-plasmid system for enhanced crispr-cas9 mediated homologous recombination

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007124915A (ja) 2005-11-01 2007-05-24 Tokai Univ リコンビニアリングコンストラクト、及びジーンターゲティングコンストラクト作製用ベクター

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4937190A (en) 1987-10-15 1990-06-26 Wisconsin Alumni Research Foundation Translation enhancer
US5464764A (en) 1989-08-22 1995-11-07 University Of Utah Research Foundation Positive-negative selection methods and vectors
IL102404A0 (en) 1992-07-03 1993-01-14 Daniel Zurr Method and means for the production of gene products
DE69433034T3 (de) 1993-04-21 2010-04-15 The University Court Of The University Of Edinburgh Expression von heterologen genen nach einem ziel-expressionsprofil
FR2722208B1 (fr) 1994-07-05 1996-10-04 Inst Nat Sante Rech Med Nouveau site interne d'entree des ribosomes, vecteur le contenant et utilisation therapeutique
WO1996011211A1 (en) 1994-10-11 1996-04-18 University Of California Selective inhibition of internally initiated rna translation
ATE303448T1 (de) * 1997-09-23 2005-09-15 Genvec Inc Duale selektionkassette und sie enthaltende plasmide
NZ514569A (en) * 1999-03-02 2004-02-27 Invitrogen Corp Compositions and methods for use in recombinational cloning of nucleic acids
US8470580B2 (en) * 2001-02-23 2013-06-25 Universite Libre De Bruxelles Method for the selection of recombination clones comprising a sequence encoding an antidote protein to a toxic molecule
CA2460617A1 (en) * 2001-08-28 2003-03-13 Japan Tobacco Inc. Method of modifying genome in higher plant
WO2005041972A1 (en) * 2003-10-31 2005-05-12 Pfizer Products Inc. Phosphodiesterase 9 inhibition as treatment for obesity-related conditions
JP2008253254A (ja) * 2007-03-14 2008-10-23 National Institutes Of Natural Sciences 高効率変異導入法
US20090111099A1 (en) * 2007-10-27 2009-04-30 Yongsheng Ma Promoter Detection and Analysis
WO2010009735A2 (en) * 2008-07-23 2010-01-28 Dako Denmark A/S Combinatorial analysis and repair
US20110212080A1 (en) 2008-08-04 2011-09-01 Umar Faruk Mansoor Urea derivatives as antibacterial agents
WO2011101696A1 (en) * 2010-02-18 2011-08-25 Cellectis Improved meganuclease recombination system
EP2678434B1 (en) * 2011-02-25 2018-11-07 Recombinetics, Inc. Genetically modified animals and methods for making the same
EP3027754B1 (en) * 2013-08-02 2022-03-02 enEvolv, Inc. Processes and host cells for genome, pathway, and biomolecular engineering
JP6374176B2 (ja) * 2014-02-13 2018-08-15 学校法人 名城大学 植物体に用いる組換えベクター及びその利用
EP3078675A1 (en) * 2015-04-10 2016-10-12 Ares Trading S.A. Induction dosing regimen for the treatment of tnf alpha mediated disorders
JP7418796B2 (ja) 2016-11-03 2024-01-22 テンプル・ユニバーシティ-オブ・ザ・コモンウェルス・システム・オブ・ハイアー・エデュケイション 細胞株の開発のための相同組換えベクターの高速生成のためのdnaプラスミド

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007124915A (ja) 2005-11-01 2007-05-24 Tokai Univ リコンビニアリングコンストラクト、及びジーンターゲティングコンストラクト作製用ベクター

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Sci Rep.,5:10710.doi: 10.1038/srep10710(2015.6.3.)*

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US10883120B2 (en) 2021-01-05
EP4219709A1 (en) 2023-08-02
KR102736524B1 (ko) 2024-11-29
JP2019537445A (ja) 2019-12-26
EP3535394B1 (en) 2023-04-12
US12359222B2 (en) 2025-07-15
IL266422A (en) 2019-06-30
EP3535394A2 (en) 2019-09-11
CN110199020B (zh) 2024-06-18
CA3081687A1 (en) 2018-08-09
KR20230148274A (ko) 2023-10-24
WO2018144087A2 (en) 2018-08-09
US20190284582A1 (en) 2019-09-19
EP3535394A4 (en) 2020-05-27
US20200002729A1 (en) 2020-01-02
KR20190113759A (ko) 2019-10-08
WO2018144087A3 (en) 2018-10-25
JP7418796B2 (ja) 2024-01-22
CN110199020A (zh) 2019-09-03

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