KR102586050B1 - Composition for skin anti-pollution containing banana peel extract as an active ingredient - Google Patents

Abstract

The present invention relates to a composition for skin anti-pollution containing banana peel extract as an active ingredient, and has an anti-pollution effect on the skin against fine dust, organic or inorganic pollutants, etc., preventing problems such as skin troubles caused by pollutants. , It can minimize skin irritation and has excellent antioxidant effects.
In addition, it has excellent skin applicability and absorption, can be used as a cosmetic formulation with high preference, and can be provided as a functional food composition with high preference.

Description

Composition for skin anti-pollution containing banana peel extract as an active ingredient}

The present invention relates to a composition for skin anti-pollution containing banana peel extract as an active ingredient.

The skin is the outermost layer and largest tissue and is exposed to external irritants such as ultraviolet rays, microorganisms, viruses, and air pollutants. It has long been studied that ultraviolet rays cause skin aging by damaging the skin.

Meanwhile, in recent years, there has been an increasing number of reported cases regarding the possibility that the adhesion of particulate air pollutants to the skin is related to aging. In addition, benzopyrene contained in cigarettes activates the aryl hydrocarbon receptor present in epidermal cells, prompting the induction of cytochrome P450 (CYP1), a drug metabolizing enzyme, and producing reactive oxygen species. It has been reported that it induces the production of interleukin 8, causing an inflammatory response.

In order to protect the skin from these external irritants, do not allow substances that can cause damage, such as particulate air pollutants, to adhere to or penetrate the skin and/or alleviate the effects of the attached irritating substances on the inside of the skin. Things to do are taken into consideration.

As a measure to protect the skin as described above, there have been proposals in the past for agents that form a film on the skin to prevent particulate air pollutants from adhering to the skin or to allow them to be easily washed off even if they do adhere to the skin. Because it is covered with a film, it feels bad when used, and it is unclear whether it is effective against all substances that cause irritation.

Studies have shown that particulate air pollutants attach to and penetrate the skin and cause oxidative stress on cells, causing inflammatory reactions, cell metabolism, and genetic damage. However, the mechanisms of cell damage include oxidation, inflammation, cell metabolism, and other factors. There are many diverse and still unknown aspects, such as abnormalities in gene expression, and perhaps for this reason, the reality is that there are not many materials on the market that have antipollution effects.

For example, it was discovered that extracts from the genus Hippophyllaceae have the effect of suppressing skin inflammation caused by atmospheric aerosol particles by suppressing the rise in PGE2 caused by atmospheric aerosol particles such as particulate air pollutants.

In addition, attention was paid to the mechanism by which pollutants penetrating inside the skin cause skin inflammation in epidermal cells, and the action of alleviating cell damage by zinc salts and/or zinc complexes of amino acids, excessive induction of CYP1, excessive induction of COX2, etc. It was found to be useful in inhibiting IL8 hyperinduction.

In addition to the above situations, there are perspectives on diversification of preparations and expansion of options in prescription formulations, synergistic effects of using anti-pollution agents against particulate air pollutants with different mechanisms, and anti-inflammatory drugs with safer and higher effectiveness. From the viewpoint of pollution agents, the development of new anti-pollution agents is required.

KR 10-2255036 B1

The purpose of the present invention is to provide a composition for skin anti-pollution containing banana peel extract as an active ingredient.

Another object of the present invention is to provide a banana peel extract that has an anti-pollution effect on the skin against fine dust, organic or inorganic pollutants, etc., can prevent problems such as skin problems caused by pollutants, minimize skin irritation, and has excellent antioxidant effects. To provide a composition for skin anti-pollution containing as an active ingredient.

Another object of the present invention is to provide a composition for skin anti-pollution containing banana peel extract as an active ingredient, which has excellent skin applicability and absorption and can be used in a highly preferred formulation.

In order to achieve the above object, the composition for skin anti-pollution according to an embodiment of the present invention may include banana peel extract as an active ingredient.

The banana peel extract may be extracted using an extraction solvent selected from the group consisting of water, C ₁ to C ₆ lower alcohols, and mixtures thereof.

The composition is excellent in suppressing the production of NO, PGE ₂ and inflammatory cytokines induced by harmful factors.

The harmful factors may be selected from the group consisting of fine dust, polycyclic aromatic hydrocarbons, aldehydes, nitrogen oxides, and mixtures thereof.

The composition includes spearmint extract, apple mint extract, peppermint extract, lemon balm extract, sweet basil extract, golden lemon thyme extract, pineapple extract, sage extract, lavender extract, rosemary extract, chamomile extract, marjoram extract, chinensis extract, and hemp leaf sprout. It may further include a natural extract selected from the group consisting of extracts and mixtures thereof.

A functional cosmetic composition according to another embodiment of the present invention may include the skin anti-pollution composition.

A functional food composition according to another embodiment of the present invention may include the skin anti-pollution composition.

Hereinafter, the present invention will be described in more detail.

“Cell protection” as used in the present invention includes the absence of cytotoxicity, excellent antioxidant activity due to high reducing power and DPPH radical scavenging ability, and the effect of suppressing reactive oxygen species production, DNA damage, apoptosis, and mitochondrial membrane damage. do.

In this specification, “extract” refers to a preparation obtained by squeezing a herbal medicine into an appropriate leachate and evaporating and concentrating the leachate. It is not limited thereto, but includes, but is not limited to, the extract obtained through extraction processing, the diluted or concentrated liquid of the extract, and the extract liquid. It may be a dried product obtained by drying, a crude product thereof, or a purified product.

In this specification, “active ingredient” refers to an ingredient that exhibits the desired activity alone or can exhibit activity in combination with a carrier that is not active on its own.

'Skin anti-pollution' as used in the present invention refers to improving skin contamination, skin cell death, inhibition of skin cell proliferation, or deterioration of skin condition caused by contaminants such as fine dust, organic pollutants, or inorganic pollutants. says

In the present invention, pollutants may include fine dust, polycyclic aromatic hydrocarbons, aldehydes, and nitrogen oxides.

A composition for skin anti-pollution according to an embodiment of the present invention includes banana peel extract as an active ingredient.

The banana is an alkaline food rich in sugar and contains a lot of potassium, carotene, and vitamin C. It is rich in pectin, which helps regulate the intestines. It is rich in dietary fiber, so it is effective in breaking down fat and eliminating constipation and stool. The potassium it contains can help prevent high blood pressure by excreting sodium from the body. Fiber is obtained from the petioles of bananas, and black dye is also collected from the mucus of the leaves.

The present invention utilizes peel (pericarp) extract, so that naturally derived compounds such as flavonoids and polyphenols can be used as active ingredients compared to pulp extracts. In addition, by using the peel of fruit that is discarded as an inedible by-product, production costs can be reduced and it is environmentally friendly.

In other words, the present invention is characterized by using banana peel, not banana flesh, as the main component, taking into account the fact that most peels generated as a by-product after processing vegetable materials such as fruits are generally discarded as waste. In this case, the present invention has eco-friendly characteristics in terms of reducing waste generation because banana flesh is manufactured as a separate product and banana peels that are scheduled to be discarded are used.

The anti-pollution means that it can suppress inflammatory reactions that may occur from external harmful environmental stimuli such as environmental harmful factors.

The inflammatory response occurs when immune cells such as macrophages and neutrophils that recognize antigens secrete various inflammatory mediators such as NO, PGE ₂ , and inflammatory cytokines.

The NO is an indicator of inflammatory response and is produced when cells are activated due to stimulation of cytokines, microbial invasion, etc., and is produced by NOS (nitric oxide synthase) from L-arginine.

The NOS exists in three forms: neuronalNOS (nNOS, NOS1), inducibleNOS (iNOS, NOS2), and endothelialNOS (eNOS, NOS3). Among these, iNOS plays an important role in regulating inflammatory responses and producing NO.

PG is produced by COX (cyclooxygenase) enzyme, and COX exists in two types, COX-1 and COX-2. COX-1 is present in most tissues and is involved in PG production, and COX-2 is a cytokine ( The expression of cytokines increases, producing a large amount of PG, thereby causing an inflammatory response.

Meanwhile, LPS (lipopolysaccharide) is a bacterial endotoxin recognized by TLR4 (toll-like receptor 4) among various external foreign substances, and is one of the major factors that cause inflammatory reactions by activating macrophages. Activated TLR4 activates two major signaling pathways, NF-ĸB and MAPK.

ERK1/2, JNK, and p38, which constitute the MAPK, activate transcriptional regulators ATF, CREB, STAT3, and NF-ĸB. Among them, NF-ĸB is an important transcription factor involved in immune responses, cell death, and inflammation. It is activated through several stages of signal transduction inside the cell, moves into the nucleus, binds to DNA, and then goes through the transcription stage. Gene expression of inflammatory proteins such as inflammatory cytokines, TNF (tumor necrosis factor)-α, IL (interleukin)-1β, and IL (interleukin)-6, and inflammatory proteins, such as COX (cyclooxygenase)-2 and iNOS (inducible nitric oxide synthase). increases and ultimately produces NO (nitric oxide), PGE ₂ (prostaglandin E ₂ ), etc., thereby mediating the inflammatory response to the inflammatory site.

The composition exhibits anti-inflammatory activity through inhibition of NO, PGE ₂ and inflammatory cytokine production.

More specifically, the production of NO and PGE ₂ can be suppressed by reducing the expression of inflammatory protein genes such as COX-2 and iNOS.

In addition, the inflammatory cytokines include IL-1, IL-6, and TNF-α, and in the present invention, they can exhibit anti-inflammatory activity through inhibiting the production of IL (interleukin)-6 and IL (interleukin)-1β. .

The NO, PGE ₂ and inflammatory cytokines are induced by environmental harmful factors.

The environmental harmful factors are selected from the group consisting of fine dust, vehicle exhaust gas, dioxin, benzopyrene, and heavy metals.

In the present invention, the environmental harmful factor may be fine dust.

The fine dust is a type of dust and refers to particulate matter that floats or blows down in the atmosphere, and is often generated when burning fossil fuels such as coal and oil or from exhaust gases such as factory vehicles. The components that make up the fine dust may vary depending on the region, season, weather conditions, etc. where the fine dust occurs, and generally, sulfates and nitrates formed when air pollutants react in the air, and those generated in the process of burning fossil fuels such as coal and oil. It is composed of soot and minerals generated from surface dirt.

Depending on the particle size, dust is divided into Total Suspended Particles (TSP), which are 50μm or less, and Particulate Matter, which is very small. The fine dust is further divided into fine dust with a diameter smaller than 10 μm (PM10) and fine dust with a diameter smaller than 2.5 μm (PM2.5). If PM10 is about 1/5 to 1/7 smaller than the diameter of a human hair (50 to 70 μm), PM2.5 is very small, only about 1/20 to 1/30 of a human hair. Accordingly, fine dust, which is so small that it is invisible to the eye, stays in the air and can infiltrate the lungs through the respiratory tract or move into the body along the blood vessels, causing adverse effects on health.

When acutely exposed to fine dust, coughing, difficulty breathing, worsening asthma, and arrhythmia occur due to airway irritation, and when chronically exposed to fine dust, lung function decreases, chronic bronchitis increases, mortality rate increases, and skin aging occurs. , can cause various diseases such as exacerbation of allergic diseases.

In addition, fine dust can cause intracellular inflammatory responses and oxidative stress.

The oxidative stress is caused by an imbalance between the production of reactive oxygen species (ROS) and the antioxidant reaction that removes them, resulting in an increase in reactive oxygen species (ROS) within cells, which oxidizes DNA, proteins, lipids, etc., damaging their structure and function. It refers to a phenomenon that causes changes and ultimately causes functional or structural damage to cells.

The banana peel extract is extracted using an extraction solvent selected from the group consisting of water, C ₁ to C ₆ lower alcohols, and mixtures thereof.

Specifically, to prepare banana peel extract, the steps include washing the banana peel; Drying after washing; Grinding banana peels after drying; leaching the pulverized material using an organic solvent; Leaching and drying the sample; Leaching using water; And including the step of leaching, a banana peel extract can be obtained.

The extract extracted using the organic solvent may further include the step of performing fractionation using the organic solvent, and the method of preparing the extract may be a conventional extraction method in the art, such as ultrasonic extraction, leaching, and reflux extraction. You can.

Specifically, it may be an extract obtained by extracting a banana peel extract from which foreign substances have been removed by washing and drying with water, alcohol with 1 to 6 carbon atoms, or a mixed solvent thereof, and may be an extract obtained by sequentially applying the solvents to the sample.

The reflux extraction method is based on 100 mL of water and alcohol with 1 to 6 carbon atoms, 10 to 30 g of pulverized natural product, reflux time of 1 to 3 hours, and 50 to 100% of alcohol or water with 1 to 6 carbon atoms. More specifically, based on 100 mL of alcohol with 1 to 6 carbon atoms or 100 mL of water, 10 to 20 g of pulverized natural product, reflux time of 1 to 2 hours, and 70 to 90% of alcohol or water with 1 to 4 carbon atoms. .

The leaching method is carried out at 15 to 30°C for 24 to 72 hours, and water or 50 to 100% alcohol with 1 to 6 carbon atoms is used as the extraction solvent. More specifically, the extraction is performed at 20 to 25°C for 30 to 54 hours, and the extraction solvent is water or 70 to 80% alcohol with 1 to 6 carbon atoms.

The ultrasonic extraction method proceeds at 30 to 50°C for 0.5 to 2.5 hours, and the extraction solvent is water or 50 to 100% alcohol with 1 to 6 carbon atoms. Specifically, extraction is performed at 40 to 50°C for 1 to 2.5 hours, and the extraction solvent is water or 70 to 80% alcohol with 1 to 6 carbon atoms.

The extraction solvent can be used in an amount of 2 to 50 times, more specifically, 2 to 20 times the weight of the sample. For extraction, the sample may be left in the extraction solvent for 1 to 72 hours, more specifically, 24 to 48 hours.

After extraction, the extract can be fractionated by sequentially applying a new fractionation solvent. The fractionating solvent used during fractionation is at least one selected from the group consisting of water, hexane, butanol, ethyl acetic acid, ethyl acetate, methylene chloride, and mixtures thereof, and is preferably ethyl acetate or methylene chloride. After obtaining the extract or fraction, additional methods such as concentration or freeze-drying can be used.

The composition for skin anti-pollution includes spearmint extract, apple mint extract, peppermint extract, lemon balm extract, sweet basil extract, golden lemon thyme extract, pineapple extract, sage extract, lavender extract, rosemary extract, chamomile extract, marjoram extract, It may further include a natural extract selected from the group consisting of L. chinensis extract, L. chinensis extract, and mixtures thereof, and preferably may further include marjoram extract, L. L. plant extract, and L. plant extract.

The marjoram (Origanum majorana) is a perennial plant of the Oysteraceae family. Its leaves are broadly egg-shaped and light yellow or white flowers bloom from June to August. It is a herb with soothing, analgesic, antiseptic, antiseptic, antispasmodic, stimulating, vasodilating, wound healing, antiviral, sterilizing, carminative, and diaphoretic effects. It has a very strong scent and is used as a flavoring and medicinal agent. It is also used as

Hemerocallis fulva L. is a perennial plant of the genus Daylily of the family Daylily and is also called daylily. It mainly grows in mountainous areas and is about 1m tall. The roots spread in all directions and thicken into a cone shape, and the leaves are arranged in two rows, about 80cm long and 1.2-2.5cm wide, with drooping ends. Flowers bloom from July to August. The flower stem grows out from between the leaves, and branches split at the end to produce 6 to 8 flowers in a raceme. The color is orange, the length is 10 to 13 cm, and the tube is 1 to 2 cm long. The bracts are line-shaped and lanceolate, 2 to 8 cm long, and the floret stems are 1 to 2 cm long. It is commonly planted for ornamental purposes, but the young shoots are eaten as vegetables and the flowers are used in Chinese cuisine.

The above-mentioned Prunella asiatica Nakai is a perennial herb that commonly grows in mountains and fields across the country and is also distributed in China and Japan. There is a rhizome. The stem is red, hairy, and 20-60cm high. The leaves are opposite each other, ovate or oval-shaped, and have smooth or slightly serrated edges. Flowers grow densely in spikes at the ends of stems from May to July, and are purple, pink, white, and lip-shaped. The calyx is shaped like a lip and is divided into five branches. The lower lip of the corolla is split into three parts. There are 4 stamens, 2 of which are long. The fruit is a small nut, splits into four pieces, and ripens to yellowish brown.

The composition contains banana peel extract as an active ingredient, and may further include marjoram extract, Chrysanthemum extract, and Lamifolia extract. Specifically, based on 100 parts by weight of the banana peel extract, it may include 10 to 20 parts by weight of marjoram extract, 10 to 20 parts by weight of Chrysanthemum extract, and 10 to 20 parts by weight of Lamifolia extract. When mixed and used within the above range, the skin anti-pollution effect increases due to the interaction between the complex extracts. When provided as a food composition, it can be provided as a food with high preference, and when provided as a cosmetic composition, it has a high preference. It provides fragrance and can be manufactured as a product with excellent spreadability.

The cosmetic composition according to another embodiment of the present invention is the banana peel?? It includes an anti-pollution composition containing an extract as an active ingredient.

The cosmetic composition of the present invention can be prepared in any formulation commonly prepared in the field, for example, solution, suspension, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, It can be formulated as powder foundation, emulsion foundation, wax foundation, and spray, but is not limited thereto. More specifically, flexible lotion, nourishing lotion, nourishing cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder formulation, oil-in-water (O/W) type or water-in-oil type. It can be prepared as a (W/O) type formulation or ointment.

In addition, in addition to the above cosmetic composition, it can be prepared in the form of an adjuvant for topical or systemic application commonly used in the art by containing a dermatologically acceptable medium or base.

Suitable cosmetic formulations that can be manufactured from the above cosmetic composition include, for example, cosmetics such as astringent lotion, softening lotion, nourishing lotion, various creams, essences, packs, foundations, etc., as well as cleansers, face washes, soaps, treatments, or beauty essences. There is.

Specific formulations include skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutritional lotion, massage cream, nutritional cream, moisture cream, hand cream, essence, nutritional essence, pack, soap, shampoo, and cleansing foam. , including formulations such as cleansing lotion, cleansing cream, body lotion, body cleanser, emulsion, pressed powder, loose powder, or eye shadow.

A food composition according to another embodiment of the present invention contains an anti-pollution composition containing the banana peel extract as an active ingredient.

The food composition according to the present invention may be provided in the form of powder, granules, tablets, capsules, syrup or beverage, and may be used with other foods or food additives, and may be used appropriately according to conventional methods. The mixing amount of the active ingredient can be appropriately determined depending on its purpose of use, such as prevention, health, or therapeutic treatment.

As a specific example, the above food composition can be used to produce processed food that can enhance anti-pollution activity against fine dust. These processed foods include, for example, snacks, beverages, alcoholic beverages, fermented foods, canned foods, processed milk foods, processed meat foods, noodles, etc. Confectionery includes biscuits, pies, cakes, bread, candy, jellies, gum, cereals, etc. Beverages include drinking water, carbonated beverages, functional ionic beverages, functional ionic beverages, juices (e.g., apple, pear, grape, aloe, tangerine, peach, carrot, tomato juice, etc.), Sikhye, etc. Alcoholic beverages include sake, whiskey, soju, beer, liquor, and fruit wine. Fermented foods include soy sauce, soybean paste, and red pepper paste. Canned food includes canned seafood (e.g., canned tuna, mackerel, saury, canned conch, etc.), canned livestock products (canned beef, pork, chicken, turkey, etc.), and canned agricultural products (canned corn, peaches, canned file apple, etc.). Milk processed foods include cheese, butter, yogurt, etc. Processed meat products include pork cutlet, beef cutlet, chicken cutlet, and sausage. Includes sweet and sour pork, nuggets, neobiani, etc. Includes noodles such as raw noodles in sealed packages. In addition, the composition can be used in retort foods, soups, etc.

The present invention has an anti-pollution effect on the skin against fine dust, organic or inorganic pollutants, etc., prevents problems such as skin problems caused by pollutants, minimizes skin irritation, and has an excellent antioxidant effect.

In addition, it has excellent skin applicability and absorption, can be used as a cosmetic formulation with high preference, and can be provided as a functional food composition with high preference.

Figure 1 shows the results of cytotoxicity evaluation according to an embodiment of the present invention.
Figure 2 shows the results of cytotoxicity evaluation according to an embodiment of the present invention.
Figure 3 shows the results of a NO production inhibition test by a composition according to an embodiment of the present invention.
Figure 4 shows the results of an experiment on inhibition of PGE ₂ production by a composition according to an embodiment of the present invention.
Figure 5 shows the results of an experiment on inhibition of cytokine production by a composition according to an embodiment of the present invention.
Figure 6 shows the results of an experiment on inhibition of cytokine production by a composition according to an embodiment of the present invention.

Hereinafter, embodiments of the present invention will be described in detail so that those skilled in the art can easily implement it. However, the present invention may be implemented in many different forms and is not limited to the embodiments described herein.

Manufacturing Example 1

Preparation of banana peel extract

The banana peel was washed, dried, and pulverized. Distilled water as an extraction solvent was added at a ratio of 1:10 (w:v), then completely immersed, and extraction was repeated three times for 3 hours while refluxing at 80°C. The extract was Whatman No. 2 Filtered with filter paper. The filtrate was concentrated under reduced pressure at 60°C to prepare a powder sample and used in the experiment. Banana peel extract (BF) was prepared by hot water extraction and concentration filtration.

Production example 2

Preparation of other natural extracts

After washing and drying marjoram, dandelion root, and honeysuckle, respectively, the extract was prepared in the same manner as the banana peel extract.

An anti-pollution composition was prepared within the weight range as shown in Table 1 below.

Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 BF 100 100 100 100 100 100 MF - 5 10 15 20 25 HF - 5 10 15 20 25 PF - 5 10 15 20 25

(Unit weight parts, MF is marjoram extract, HF is ginseng extract, PF is Lamifolia extract)

Experimental Example 1

Cytotoxicity evaluation

RAW 264.7 cells were purchased from Korea Cell Line Bank (Korea) and incubated with Dulbeccommodia containing 10% (v/v) fetal bovine serum (FBS), 100 U/ml penicillin, and 100 U/ml streptomycin. The cells were cultured at 37°C and 5% CO ₂ (v/v) using Pied Eagle medium (in Dulbecco's modified Eagle's medium).

The cytotoxicity of banana peel extract (BF) to the RAW 264.7 cells was measured through MTT reduction assay.

Cells were treated with various concentrations of banana peel extract (BF). Afterwards, the medium was removed, and 0.5 mg/ml of MTT solution (Sigma-Aldrich Chemical Co.) was distributed to each well and reacted at 37°C. After incubation for 3 hours, the supernatant was removed and DMS was added to dissolve the blue formazan crystals for 10 minutes. The absorbance per well was measured at a wavelength of 540 nm using an enzyme-linked immunosorbent assay (ELISA) plate reader (Dynatech Laboratories, Chantilly, VA, USA).

Referring to Figure 1, it was confirmed that there was no cytotoxicity to RAW 264.7 cells at a concentration of 500 μg/ml or less of banana peel extract (BF), and Figure 2 shows standard diesel particulate matter (SRM 1650b, hereinafter PM2. It was confirmed that administering less than 500 μg/ml of banana peel extract (BF) to RAW 264.7 cells treated with (referred to as 5) did not have a significant adverse effect on cell survival.

Below, the concentration of banana peel extract (BF) was confirmed to have a skin anti-pollution effect at 500 μg/ml, and 500 μg/ml was also used as the standard when manufacturing the complex extract.

NO, PGE ₂ and measurement of cytokines.

RAW 264.7 cells were treated with 200 μg/ml and 500 μg/ml banana peel extract for 1 hour and then stimulated with 50 μg/ml PM2.5 for 24 hours. NO levels in the medium were assessed by the amount of nitrite measured using Griess reagent (Sigma-Aldrich Chemical Co.). 100 μL of cell conditioned medium was mixed with an equal amount of Griess reagent for 10 minutes. Absorbance was measured at 540 nm using an ELISA reader and calculated by comparison with a sodium nitrite (NaNO ₂ ) standard curve.

Additionally, to examine PGE ₂ and cytokine levels, culture supernatants were collected and evaluated using an ELISA kit (R & D Systems Inc., Minneapolis, MN, USA). Absorbance was measured at a wavelength of 450 nm using an ELISA reader.

The results of the above experiment show that, as shown in Figures 3 and 4, PM2.5 treatment significantly increased the release of NO and PGE ₂ compared to the control group that was not treated with PM2.5, but this increase was due to the treatment concentration of banana peel extract. As it increased, it decreased significantly.

Afterwards, we investigated whether banana peel extract could inhibit the expression of iNOS and COX-2 by PM2.5. Referring to Figures 3 and 4 below, it was confirmed that the mRNA and protein expressions of iNOS and COX-2 increased by PM2.5 were significantly suppressed in the presence of banana peel extract (BF) according to RT-PCR and Western blot results. did.

Additionally, the effect of banana peel extract on the production and expression of pro-inflammatory cytokines increased by PM2.5 treatment is shown in Figures 5 and 6. Specifically, it was confirmed that after stimulation by PM2.5, the amount of pro-inflammatory cytokines including IL-6 and IL-1β released into the culture supernatant significantly increased. However, it was confirmed that the production of pro-inflammatory cytokines by PM2.5 was significantly inhibited by pretreatment with banana peel extract.

Experimental Example 2

Skin anti-pollution effect of complex extract

According to the results of confirming the skin anti-pollution effect of the banana peel extract, the skin anti-pollution effect of the complex extract was tested in the same manner.

In the cytotoxicity results, it was confirmed that there was no cytotoxicity to RAW 264.7 cells even when MF, HF, and PF were treated at the same concentration (500 μg/ml), and there was also no cytotoxicity when treated with the mixed complex. Confirmed.

Afterwards, experiments were also conducted to measure NO, PGE ₂ , and cytokines. The above experiment is the result of the experiment for Example 1. For relative comparison, Example 1 was set to an index of 5, and Examples 2 to 6 were evaluated by the index.

The results are shown in Table 2 below.

Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Inhibition of NO and PGE ₂ expression 5 3 6 8 8 4 Inhibition of inflammatory cytokine (IL-6, IL-1β) production 5 3 6 9 7 5

(unit exponent)

According to the above experimental results, it was confirmed that compared to Example 1, Examples 3 to 5 showed an equally excellent effect of suppressing NO and PGE ₂ expression and were also excellent in suppressing inflammatory cytokine production.

Sensory evaluation

A moisturizing cream was prepared in a conventional manner using the example composition as follows. Afterwards, 20 men and women were asked to evaluate the scent, applicability, and moisturizing effect.

Example composition 3.0% by weight

Glycerin 8.0% by weight

Butylene glycol 5.0% by weight

Glyceryl stearate/PEG-100 stearate 1.0% by weight

Polysorbate 60 0.5% by weight

Sorbitan stearate 0.5% by weight

Phytosqualane 5.0% by weight

Jojoba oil 3.0% by weight

Caprylic/capric triglyceride 15.0% by weight

Aloe butter 2.0% by weight

Beeswax 1.0% by weight

Stearyl alcohol 0.5% by weight

Beheryl alcohol 0.5% by weight

Stearic acid 0.5% by weight

Carboxyl vinyl polymer 0.2% by weight

Xanthan gum 0.1% by weight

Arginine 0.5% by weight

Dimethicone 0.5% by weight

Beta-glucan 0.2% by weight

Preservative 0.5% by weight

Purified water remaining

The evaluation of each item was requested based on a 10-point scale, and the evaluation results were derived as the average value.

Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 incense 5 5 6 8 7 4 Spreadability 6 7 8 8 9 3 Moisturizing 8 6 7 8 8 7

According to the above experimental results, it was confirmed that Examples 3 to 5 received excellent evaluations in fragrance, spreadability, and moisturizing properties, and also received excellent evaluations in overall preference.

Although the preferred embodiments of the present invention have been described in detail above, the scope of the present invention is not limited thereto, and various modifications and improvements made by those skilled in the art using the basic concept of the present invention defined in the following claims are also possible. falls within the scope of rights.

Claims (7)

Contains banana peel extract, marjoram extract, seaweed extract, and honeysuckle extract as active ingredients.
Composition for skin anti-pollution. According to paragraph 1,
The banana peel extract is extracted using an extraction solvent selected from the group consisting of water, C ₁ to C ₆ lower alcohols, and mixtures thereof.
Composition for skin anti-pollution. According to paragraph 1,
The composition has an excellent effect of suppressing the production of NO, PGE ₂ and inflammatory cytokines induced by harmful factors.
Composition for skin anti-pollution. According to paragraph 3,
The harmful factors are selected from the group consisting of fine dust, polycyclic aromatic hydrocarbons, aldehydes, nitrogen oxides, and mixtures thereof.
Composition for skin anti-pollution. delete Comprising the composition for skin anti-pollution according to any one of claims 1 to 4.
Functional cosmetic composition. Comprising the composition for skin anti-pollution according to any one of claims 1 to 4.
Functional food composition.