KR102525622B1 - Method of Producing Adlay Oil and Cosmetic Composition for Skin Whitening Comprising Adlay Oil Produced by the Method - Google Patents

Abstract

The present invention relates to a method for preparing adlay oil, and more particularly, to a method for preparing adlay oil comprising roasting adlay, and a cosmetic composition for skin whitening containing adlay oil prepared by the method.
Adlay oil prepared by the manufacturing method according to the present invention has a high milking yield, excellent tyrosinase inhibitory activity and DPPH radical scavenging activity, and uses safe natural substances, so it can be used in functional cosmetics for suppression of melanin production and skin whitening. can be useful In addition, through the diversification of products using adlay, it is possible to expand the consumption of adlay and improve added value.

Description

Method of Producing Adlay Oil and Cosmetic Composition for Skin Whitening Comprising Adlay Oil Produced by the Method}

The present invention relates to a method for preparing adlay oil, and more particularly, to a method for preparing adlay oil comprising roasting adlay, and a cosmetic composition for skin whitening containing adlay oil prepared by the method.

The scale of domestic and foreign cosmetics and cosmetic materials market is growing rapidly every year. The global cosmetics industry is expected to grow at an average annual rate of 5% by 2022, reaching $464.8 billion in 2017 (2018 Global Cosmetics Industry White Paper (KOTRA)), and Euromonitor predicted that dermatology (with functional ingredients) among global cosmetic types in 2017 The growth rate of the dermatology market was 5.7% compared to the previous year, exceeding the overall growth rate of cosmetics (5.0%).

On the other hand, as interest in beauty increases, interest in whitening functional cosmetics for improving dull skin and relieving blemishes such as freckles and freckles is increasing, and accordingly, research on the development of whitening materials is being actively conducted.

The synthesis of melanin is achieved by making melanosomes in melanocytes present in the basal layer of the skin, and a series of processes in which melanin is synthesized are collectively referred to as melanogenesis. Melanin production uses tyrosine, one of the amino acids, as a substrate, and DOPA (3, After conversion to DOPA quinone through 4-dihydroxyphenylalanine), melanin is synthesized by polymerization with amino acids or proteins after undergoing a non-enzymatic reaction and spontaneous oxidation process (Korean J. FOOD SCI. TECHNOL., 2000. 32(3):736;Journal of Life Science, 2013.23(12):1445;Pigment Cell Res.1999.12(1):4-12.).

Therefore, since tyrosinase plays the most important role in the first step of melanin production, studies on whitening agents are mainly focused on tyrosinase activity inhibitors or tyrosinase substrates to regulate tyrosinase activity. Research has been conducted in the direction of developing antagonists (Kor. J. Pharmacogn., 44(3):220, 2013). Currently, substances widely used as whitening agents include ascorbic acid, kojic acid, arbutin, hydroquinone, and the like. However, ascorbic acid is not suitable as a melanin production inhibitor due to its low tyrosinase activity inhibitory effect and low stability. Kojic acid and arbutin have excellent tyrosinase activity inhibitory effects, but are stable due to side effects such as oxidation and discoloration and skin irritation when formulated in cosmetics. have a problem with In addition, hydroquinone has safety concerns due to its toxicity to the skin. Therefore, there is a demand for the development of whitening materials that are not toxic to the skin and have an excellent melanin production inhibitory effect, and accordingly, natural materials with various active ingredients and excellent safety are attracting attention.

In particular, natural substances often have antioxidant activity, and antioxidant activity suppresses the oxidation of DOPA quinone in the melanin production process to show whitening activity. can induce an increase in activity (J. Am. Acad. Dermatol. 3: 99-105, 1994).

On the other hand, with respect to the composition containing adlay as a natural substance having whitening activity, a composition for skin whitening using adlay rice bran extract or its fermented product (KR 10-2124255) and a whitening composition containing adlay root extract (KR 10- 1579500), etc. have been studied, but these are extracts extracted from rice bran or root of adlay using a solvent such as water or ethanol, and there has been no report on adlay oil prepared by roasting and then pressing adlay.

Accordingly, the present inventors have made diligent efforts to find a natural substance with low concern for human toxicity and excellent skin whitening effect. The present invention was completed by confirming that there is an effect of inhibiting synase inhibition and melanin production.

The above information described in this background section is only for improving the understanding of the background of the present invention, and therefore does not include information that forms prior art known to those skilled in the art to which the present invention belongs. may not be

An object of the present invention is to provide a method for preparing adlay oil having excellent skin whitening efficacy.

Another object of the present invention is to provide a cosmetic composition for whitening containing adlay oil prepared by the above method.

In order to achieve the above object, the present invention is (a) roasting the washed adlay at a temperature of 130 ~ 150 ℃; And (b) providing a method for preparing adlay oil comprising the step of obtaining adlay oil by squeezing and filtering the roasted adlay.

The present invention also provides a cosmetic composition comprising adlay oil and a carrier oil prepared by the above method.

Adlay oil prepared by the manufacturing method according to the present invention has a high milking yield, excellent tyrosinase inhibitory activity and DPPH radical scavenging activity, and uses safe natural substances, so it can be used in functional cosmetics for suppression of melanin production and skin whitening. can be useful In addition, through the diversification of products using adlay, it is possible to expand the consumption of adlay and improve added value.

1 is a schematic diagram showing the manufacturing process of adlay oil of the present invention.
Figure 2 shows that the skin whitening clinical test by manufacturing a test product with 40% by weight of Coix oil content shows that the melanin pigment level is improved by 4.76% compared to the control product after 4 weeks of use (Example 3).
Figure 3 shows that when the test product was used for 4 weeks, melanin production was inhibited and there was a whitening effect compared to the control product (Example 3).

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. In general, the nomenclature used herein is one well known and commonly used in the art.

In one embodiment of the present invention, after washing the adlay, it was roasted until it reached 140 ° C based on the temperature of the grain (adlay) and then compressed in a press at a pressure of 600 kgf / cm 2 . Thereafter, the supernatant was separated by centrifugation, firstly filtered with a pore size of 22 μm, secondly filtered with a pore size of 1 μm, and thirdly filtered with a pore size of 0.8 μm to obtain Adlay Oil was obtained (Example 1).

Therefore, the present invention from one aspect (a) roasting the washed adlay at a temperature of 130 ~ 150 ℃; And (b) it relates to a method for producing adlay oil comprising the step of obtaining adlay oil by squeezing and filtering the roasted adlay.

In the present invention, the temperature of step (a) may be characterized in that 140 ℃.

In the present invention, the temperature of step (a) means roasting until the temperature of adlay is 130 ~ 150 ℃. If the adlay is not roasted, it is not milked in brown rice and adlay, and even when roasted at less than 120 ° C, compression is impossible (Examples 1-1 and 1-2). When roasted at 130 ° C. or lower, the milk yield is low and the tyrosinase inhibitory ability is low, and when roasted at 150 ° C. or higher, the tyrosinase inhibitory ability is significantly lowered (Table 3).

In the present invention, the compression in step (b) may be characterized in that the compression is performed at a pressure of 400 to 800 kgf/cm 2 , preferably at a pressure of 600 kgf/cm 2 .

In the present invention, a step of separating the supernatant by centrifugation after compression in step (b) may be further included.

In the present invention, the filtration of step (b) is performed by primary filtration with a pore size of 15 to 25 μm; Secondary filtration with a pore size of 0.5 to 1.5 μm; and tertiary filtration with a pore size of 0.3 to 0.9 μm, preferably, primary filtration with a pore size of 22 μm; Secondary filtration with a pore size of 1 μm; and tertiary filtration with a pore size of 0.8 μm.

In the present invention, the adlay oil may be characterized as having one or more of the following characteristics:

1) increased tyrosinase inhibition;

2) increased DPPH radical scavenging activity;

3) increased milk yield;

4) increased chromaticity L value; and

5) Increased content of saturated and unsaturated fatty acids.

Fatty acids include saturated fatty acids and unsaturated fatty acids, saturated fatty acids include palmitic acid, and unsaturated fatty acids include oleic acid, linoleic acid, alpha linolenic acid, and the like. Palmitic acid, oleic acid, linoleic acid, alpha linolenic acid, etc. are known to exhibit moisturizing properties such as helping the skin to retain moisture by constructing the skin barrier (Carrasco, F. (2009). 〈Ingredientes Cosmιticos〉. 《Diccionario de Ingredientes, 4th ed., page 428; Diezel, W.E.; Schulz, E.; Skanks, M.; Heise, H. (1993) "Plant oils: Topical application and anti-inflammatory effects (croton oil test)". Dermatologische Monatsschrift” 179: 173.).

DPPH (1,1-diphenyl-2-picrylhydrazyl) radical is a very stable free radical. Antioxidant effect was evaluated as the degree of scavenging this radical.

In the present invention, the term "Adlay (Coix lachryma-jobi var. mayuen (Roman.) Stapf.)" is an annual herbaceous plant belonging to the Poaceae family, its flowers bloom in July and its fruits are well known to the general public as edible. In oriental medicine, it is called uiyiin and is also used as medicine.

The nutritional components of adlay are shown in Table 1 below, and the fatty acid composition of adlay (raw material) is shown in Table 2.

Nutritional content of Adlay (unit: g/100g, dry matter weight) Mixed grains crude protein crude fat views crude fiber Adlay Mugyeo 15.87 20.21 6.28 18.36 Brown rice adlay 16.41 6.86 1.94 6.41 adlay rice 14.65 6.04 1.74 5.15

Fatty Acid Composition of Adlay (Raw Material) fatty acid name Fatty acid composition (%) Adlay Mugyeo Brown rice adlay adlay rice saturated fatty acids 17.3±0.0 16.9±0.1 31.6±0.2 Myristic acid C14:0 0.1±0.0 - 0.1±0.0 Palmitic acid C16:0 15.0±0.0 14.1±0.0 26.4±0.2 Margaric acid C17:0 0.2±0.0 0.1±0.0 0.2±0.0 Stearic acid C18:0 1.7±0.0 2.0±0.0 3.9±0.0 Arachidic acid C20:0 0.3±0.0 0.3±0.0 0.6±0.0 Behenic acid C22:0 - 0.1±0.0 0.2±0.0 Lignoceric acid C24:0 - 0.1±0.0 0.2±0.0 unsaturated fatty acids 82.7±0.0 83.1±0.3 68.4±0.6 Palmitoleic acid C16:1 0.2±0.0 0.2±0.0 0.3±0.0 Margaroleic acid C17:1 - - 0.1±0.0 Oleic acid C18:1 42.0±0.0 45.3±0.2 - Linoleic acid C18:2 39.4±0.0 36.8±0.1 66.5±0.6 Linolenic acid C18:3 0.9±0.0 0.6±0.0 0.9±0.0 Eicosenic acid C20:1 0.2±0.0 0.2±0.0 0.4±0.0 Eicosadienoic acid C20:2 - - 0.1±0.0 Erucicacid C22:1 - - 0.1±0.0

In Table 1, Coal radish bran refers to the peeled skin of Coal radish after peeling the Coal radish, and is used in combination with Coal radish rice bran. Brown rice adlay is peeled adlay, which is not removed from the skin, and adlay rice is the bran layer removed from brown adlay, and is generally used for mixing.

As shown in Table 1 above, the crude fat content of coix radish is about 20%, whereas brown rice and coix radish have a low fat content of 6 to 7%. do. When unroasted adlay is pressed, oil may be absorbed into the starch contained in adlay, so that adlay oil may not be extracted.

When roasting and then pressing adlay, oil is extracted from all three types of adlay bran, brown rice, and adlay, and oil can be extracted only from unroasted adlay bran. As shown in Table 2, brown rice radish has the highest composition of unsaturated fatty acids such as oleic acid.

From another point of view, the present invention relates to adlay oil prepared by the above production method.

In one embodiment of the present invention, when 10 to 20% by weight of adlay oil was mixed with jojoba oil (a type of carrier oil), tyrosinase inhibitory activity and DPPH radical scavenging activity were the highest, and 20 to 40 When mixed by weight %, the highest tyrosinase inhibitory activity was shown, and the DPPH radical scavenging activity was also high.

Therefore, from another aspect, the present invention relates to a cosmetic composition comprising adlay oil and a carrier oil prepared by the above method.

In the present invention, the composition may be characterized in that it is for whitening.

In the present invention, the composition may be characterized in that it is for antioxidant.

In the present invention, the composition may be characterized by comprising 10 to 40% by weight of adlay oil and 60 to 90% by weight of a carrier oil, preferably 40% by weight of adlay oil and 60% by weight of a carrier oil, more preferably 40% by weight of adlay oil, 54% by weight of a carrier oil, 1% by weight of tocopherol and 5% by weight of fragrance.

In the case of mixed oil containing 90% by weight or more of carrier oil (content of adlay oil 10% by weight or less), tyrosinase inhibitory activity and DPPH radical scavenging activity were low (Table 6), and carrier oil was 60% by weight or less In the case of mixed oil containing (content of adlay oil of 40% by weight or more), the inhibitory ability of tyrosinase was significantly lowered (Table 7).

In the present invention, the carrier oil may be jojoba oil, argan oil, or camellia oil, preferably jojoba oil or camellia oil, and more preferably jojoba oil.

In the present invention, the composition may further include tocopherol or fragrance.

In the present invention, the term "whitening" refers to a method of increasing the brightness of skin whose brightness is reduced due to an excess of pigments such as melanin or maintaining the brightness of the skin at a certain level, and skin with increased brightness formed by the above method. means inclusive.

The cosmetic composition for whitening of the present invention may be prepared in any formulation commonly prepared in the art, for example, a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant - It may be formulated into cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray, etc., but is not limited thereto. More specifically, the cosmetic composition for whitening of the present invention is a formulation of softening lotion, astringent lotion, nutrient lotion, nutrient cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder. can be manufactured.

When the cosmetic formulation for whitening of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tragacanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or Zinc oxide and the like can be used. When the cosmetic formulation for whitening of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. When the cosmetic formulation for whitening of the present invention is a solution or emulsion, a solvent, solubilizing agent or emulsifying agent is used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzo Fatty acid esters of ethyl alcohol, propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic esters, polyethylene glycol or sorbitan may be used. When the cosmetic formulation for whitening of the present invention is a suspension, water, a liquid diluent such as ethanol or propylene glycol, an ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester as carrier components. A suspending agent, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tragacanth, and the like may be used. When the cosmetic formulation for whitening of the present invention is a surfactant-containing cleanser, as carrier components, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, and sarcosyl nates, fatty acid amide ether sulfates, alkylamidobetaines, fatty alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

In addition, the cosmetic composition for whitening of the present invention may contain a surfactant. Surfactants can be anionic surfactants, cationic surfactants, nonionic surfactants or compatible mixtures of surfactants. Surfactants can also be ampholytic or amphoteric surfactants with anionic or cationic properties depending on the pH of the composition.

In addition, the cosmetic composition for whitening of the present invention may also contain a hydrotrope. Hydrotropes are compounds that have the ability to enhance the water solubility of other compounds. Specific examples of hydrotropes include, but are not limited to, sodium cumene sulfonate, ammonium cumene sulfonate, ammonium xylene sulfonate, potassium toluene sulfonate, sodium toluene sulfonate, sodium xylenesulfonate, toluene sulfonic acid and xylene sulfonic acid. Other useful hydrotropes include sodium polynaphthalene sulfonate, sodium polystyrene sulfonate, sodium methyl naphthalene sulfonate, sodium camphor sulfonate, and disodium succinate.

In addition, the cosmetic composition for whitening of the present invention may include a thickening agent or a gelling agent. Thickeners or gelling agents are, for example, polymers or copolymers unsaturated carboxylic acids or unsaturated esters, polysaccharide derivatives, gums, colloidal silicates, polyethylene glycol (PEG) and its derivatives, polyvinylpyrrolidone and its derivatives, polyacrylamide and its derivatives. derivatives, polyacrylonitrile, hydrophilic silica gel or mixtures thereof.

In addition, the cosmetic composition for whitening of the present invention may be prepared by including a skin whitening component known in the art, a skin wrinkle improvement component, a UV protection component, a skin moisturizing component, etc. can Specifically, skin whitening ingredients include arbutin, niacinamide, ascorbyl glucoside, alpha-bisabolol, oil soluble licorice (Glycyrrhiza) Extract, and the like, and skin wrinkle improvement ingredients include retinol and retinyl palmitate. tate, adenosine, polyethoxylated amide, and the like, and examples of UV protection ingredients include drometrizole, drometrizoletrisiloxane, digaloyltrioleate, dimethicodyethylbenzalmalonate, and diethylhexylbutami. Compounds such as dotria zone and pine bark extract, phosphatidylserine, finger root extract powder, complex extracts such as red ginseng and cornus officinalis, and the like. In addition, as moisturizing ingredients, AP collagen enzyme decomposition peptide, Collactive collagen peptide, N-acetylglucosamine, konjac potato extract, complex extracts such as dandelion, rice bran extract, corn germ extract, low molecular weight collagen peptide, weed extract powder, phosphatidylserine, hyaluronic acid, etc. can be heard For other skin-whitening ingredients, wrinkle-improving ingredients, UV protection ingredients, and skin-moisturizing ingredients, the functional cosmetics code according to the Korean Cosmetics Act (Functional Cosmetics Standards and Test Methods in the Ministry of Food and Drug Safety’s notice) and the Health Functional Foods Act of Korea Reference can be made to the Functional Food Codex (MFDS Notice 「Standards and Specifications for Health Functional Foods」).

Hereinafter, the present invention will be described in more detail through examples. These examples are only for illustrating the present invention, and it will be apparent to those skilled in the art that the scope of the present invention is not to be construed as being limited by these examples.

Example 1: Manufacturing method of adlay oil

After washing and dehydrating the adlay, it was roasted until it reached 140 ° C based on the temperature of the grain (adlay), and then compressed in a press at a pressure of 600 kgf / cm 2 . Thereafter, the supernatant was separated by centrifugation, firstly filtered with a pore size of 22 μm, secondly filtered with a pore size of 1 μm, and thirdly filtered with a pore size of 0.8 μm to obtain Adlay Oil was obtained.

Example 1-1: Characteristics of adlay oil according to extraction method and roasting temperature

go. General characteristics and functionality

As shown in Tables 3 and 4, adlay oil prepared by roasting adlay at 140 ° C based on the grain temperature and then pressing it has the highest milking yield than when roasting at other temperatures, color L value, polyunsaturated fatty acid, DPPH radical scavenging Activity and tyrosinase inhibition were the highest.

Tyrosinase inhibitory ability was calculated by measuring absorbance at 510 nm after 15 minutes of reaction ((EC - S) / EC X 100).

General Characteristics and Functionality of Adlay Oil division milking
transference number
(%) turbidity chromaticity Tyrosinase
Inhibition (%) DPPH
Radical scavenging activity
(%) polyphenols
(ppm) L a b milking method Stir-fry temperature pressure 120℃ 0.95 181.3 73.48 -0.36 27.46 17.1ab 80.3a 833d 140℃ 2.81 105.7 75.90 -2.35 28.38 27.4a 82.7a 641e 160℃ 2.71 85.0 71.30 1.68 30.75 14.0b 80.6a 1,261c 180℃ 1.66 128.7 61.07 13.81 27.73 8.3b 59.5b 1,524b ethanol extraction 3.10 42.2 84.49 -8.44 39.27 -20.2c -72.9c 2,385a

me. fatty acid

As shown in Tables 3 and 4, Coix oil prepared by roasting at 140 ° C and then pressing was significantly superior to DPPH radical scavenging activity and tyrosinase inhibitory activity compared to ethanol extraction, polyunsaturated fatty acids and fatty acids known to have moisturizing function (Palmitic acid, oleic acid, linoleic acid, alpha linolenic acid) content was high.

The common names and chemical formulas of fatty acids are as follows: palmitic acid (C16:0), oleic acid (C18:1(n-9)), linoleic acid (C18:2(n-6)c), alpha linolenic acid (C18:3 (n-3)).

Fatty acid content of adlay oil division Composition of saturated and unsaturated fatty acids (%) Major Fatty Acids (%) milking method Stir-fry temperature saturation single
unsaturated approach
unsaturated trans palmitic acid oleic acid linoleic acid alpha linolenic acid etc pressure 120℃ 15.9c 41.4e 42.7b 0.1 12.8 41.0 34.3 8.1 3.8c 140℃ 15.7d 41.5d 42.8a 0.0 12.9 41.1 34.7 7.8 3.4d 160℃ 15.9c 41.9c 42.1c 0.1 12.7 41.6 35.8 6.1 3.8b 180℃ 17.1b 42.6b 40.4d 0.1 13.5 42.1 35.6 4.6 4.2a ethanol extraction 17.2a 44.6a 38.2e 0.0 14.5 44.1 37.0 0.6 3.9bc

Example 1-2: Adlay Oil Characteristics with or without Roasting

As shown in Table 5, Adlay oil prepared by roasting adlay at 140 ° C. based on the grain temperature and then pressing it showed significantly higher tyrosinase inhibitory activity and DPPH radical scavenging activity compared to oil obtained by cold-pressing adlay bran.

In the case of including the step of roasting adlay, it is interpreted that the same result is shown because oil is milked in brown rice adlay.

In addition, since the bran is in the form of powder, it cannot be roasted, and if it is not roasted, it is not milked from brown rice.

Oil characteristics according to whether roasted or not and type of adlay division milking
transference number
(%) turbidity chromaticity Tyrosinase
Inhibition (%) DPPH
Radical scavenging activity
(%) Poly
phenol
(ppm) Adlay type Milking method and
Stir-fry temperature L a b Brown rice adlay Stir-fry at 140℃ and press 2.81 105.7 75.90 -2.35 28.38 23.1 19.2 332.8 Adlay Mugyeo cold pressed 5.92 123.0 76.34 -0.92 34.55 3.8 -20.1 404.4

Example 2: Functionality of oil according to mixture of adlay oil and carrier oil

Jojoba oil, argan oil, and camellia oil, which are widely used in face oil and are quickly absorbed into the skin, are mixed with 0 to 20% by weight of adlay oil to examine the functionality of the oil. When mixed over, tyrosinase inhibition was the highest, and argan oil had higher tyrosinase inhibition as more joblay oil was added (Table 6).

Functionality of oil mixed with 0-20% by weight of adlay oil in carrier oil division Tyrosinase
Inhibition (%) DPPH radical scavenging activity (%) Polyphenols (ppm) carrier oil type Adlay Oil Mixing Ratio jojoba oil 0% 29.0b 40.9d 84.3e 5% 37.1b 35.3e 121.7c 10% 50.8a 52.2c 128.4b 15% 49.0a 62.0b 101.2d 20% 54.5a 65.6a 145.6a argan oil 0% 6.7d 23.5e 27.0d 5% 18.2c 26.7d 38.8c 10% 21.9bc 55.0c 93.1a 15% 29.0b 56.8b 89.2b 20% 37.8a 67.5a 92.3a camellia oil 0% 37.2c 42.5d 135.0b 5% 62.2b 59.2c 66.5e 10% 65.2a 60.1bc 87.2d 15% 66.5a 61.0b 126.7c 20% 65.1a 65.8a 137.4a

As a result of analysis by mixing jojoba oil with adlay oil mixing ratio of 0 to 100% by weight, mixing jojoba oil with 20 to 40% by weight of adlay oil showed the highest tyrosinase inhibitory activity.

Functionality of oil mixed with jojoba oil and adlay oil in an amount of 0 to 100% by weight division Tyrosinase
Inhibition (%) DPPH radical scavenging activity (%) Polyphenols (ppm) carrier oil type Adlay Oil Mixing Ratio jojoba oil 0% 6.2d 36.6e 83f 20% 46.3a 60.7d 122e 40% 50.1a 66.7c 209c 60% 30.9b 71.6a 199d 80% 26.8bc 71.4a 287a 100% 21.2c 67.6b 257b

Based on this, when manufacturing adlay oil as a whitening functional cosmetic, mixing 10-40% by weight of adlay oil with 90-60% by weight of carrier oil such as jojoba oil and camellia oil was predicted to have the highest whitening effect.

Example 3: Skin whitening clinical efficacy test using adlay oil ampoule

For the clinical efficacy test, the functionality of the control product and the test product was compared. The test product was composed of 40% of Coix oil + 30% of Camellia oil + 24% of jojoba oil + 1% of tocopherol + 5% of fragrance, and the control product used caprylic/capric triglyceride instead of Coix oil.

go. Functional comparison

Compared to the control product, the test product showed significantly higher tyrosinase inhibitory activity and DPPH radical scavenging activity.

Functional comparison of control product and test product division Tyrosinase
Inhibition (%) DPPH radical scavenging activity (%) Polyphenols (ppm) Control product -70.1 59.9 532 test product 20.0 71.2 194

me. Clinical efficacy test results

Six women between the ages of 40 and 59 were applied twice a day for 4 weeks (half-test).

As a result of a skin whitening clinical test by manufacturing a test product with 40% by weight of coix oil, the melanin pigment level was improved by 4.76% compared to the control product after 4 weeks of use (FIG. 2).

In addition, as a result of measuring the image of the 50-year-old test subject S01, it was confirmed that when the test product was used for 4 weeks, melanin production was inhibited and the whitening effect was compared to the control product (FIG. 3).

As above, specific parts of the present invention have been described in detail, and it will be clear to those skilled in the art that these specific descriptions are merely preferred embodiments, and the scope of the present invention is not limited thereby. will be. Accordingly, the substantial scope of the present invention will be defined by the appended claims and their equivalents.

Claims (12)

(a) roasting the washed adlay at a temperature of 140 ° C; and
(b) A method for producing adlay oil comprising the step of obtaining adlay oil by squeezing and filtering the roasted adlay at a pressure of 400 to 800 kgf/cm 2 .
delete delete The method of claim 1, further comprising separating the supernatant by centrifugation after pressing in step (b).
The method of claim 1, wherein the filtration in step (b) comprises: first filtering with a pore size of 15 to 25 μm; Secondary filtration with a pore size of 0.5 to 1.5 μm; And Adlay oil manufacturing method comprising the step of tertiary filtration with a pore size of 0.3 ~ 0.9㎛.
The method according to claim 1, wherein the adlay oil has one or more of the following characteristics:
1) increased tyrosinase inhibition;
2) increased DPPH radical scavenging activity;
3) increased milk yield;
4) increased chromaticity L value; and
5) Increased content of saturated and unsaturated fatty acids.
A cosmetic composition comprising Adlay oil and a carrier oil prepared by the method of any one of claims 1 and 4 to 6.
The cosmetic composition according to claim 7, wherein the composition is for whitening.
The cosmetic composition according to claim 7, wherein the composition is for antioxidation.
The cosmetic composition according to claim 7, wherein the composition comprises 10 to 40% by weight of adlay oil and 60 to 90% by weight of a carrier oil.
The cosmetic composition according to claim 7, wherein the carrier oil is jojoba oil, argan oil, or camellia oil.
The cosmetic composition according to claim 7, further comprising tocopherol or fragrance.

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