KR102519502B1 - 만능성 줄기 세포의 현탁 배양 - Google Patents

만능성 줄기 세포의 현탁 배양 Download PDF

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KR102519502B1
KR102519502B1 KR1020217022958A KR20217022958A KR102519502B1 KR 102519502 B1 KR102519502 B1 KR 102519502B1 KR 1020217022958 A KR1020217022958 A KR 1020217022958A KR 20217022958 A KR20217022958 A KR 20217022958A KR 102519502 B1 KR102519502 B1 KR 102519502B1
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KR20210094141A (ko
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벤자민 프라이어
다이나 라니아우스카스
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얀센 바이오테크 인코포레이티드
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells

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KR1020217022958A 2014-12-19 2015-12-09 만능성 줄기 세포의 현탁 배양 Active KR102519502B1 (ko)

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US201462094509P 2014-12-19 2014-12-19
US62/094,509 2014-12-19
PCT/US2015/064713 WO2016100035A1 (en) 2014-12-19 2015-12-09 Suspension culturing of pluripotent stem cells
KR1020177019548A KR102281752B1 (ko) 2014-12-19 2015-12-09 만능성 줄기 세포의 현탁 배양

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US (1) US20160215268A1 (enExample)
EP (1) EP3234109A4 (enExample)
JP (1) JP6800854B2 (enExample)
KR (3) KR102519502B1 (enExample)
CN (1) CN107250349A (enExample)
AR (1) AR103080A1 (enExample)
AU (1) AU2015363008B2 (enExample)
BR (1) BR112017012974A2 (enExample)
CA (1) CA2970935A1 (enExample)
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RU (1) RU2017125728A (enExample)
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TW (1) TW201636421A (enExample)
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Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3408375A4 (en) 2016-01-26 2019-07-17 Centre for Commercialization of Regenerative Medicine PROCESS FOR DISSOCIATION OF CELL AGGREGATES
US10391156B2 (en) 2017-07-12 2019-08-27 Viacyte, Inc. University donor cells and related methods
JP2020527956A (ja) * 2017-07-21 2020-09-17 センマ・セラピューティクス・インコーポレーテッド 幹細胞由来の膵臓ベータ細胞の再凝集
US20200199540A1 (en) * 2017-09-11 2020-06-25 Novo Nordisk A/S Enrichment of nkx6.1 and c-peptide co-expressing cells derived in vitro from stem cells
CN118497104A (zh) 2017-11-15 2024-08-16 沃泰克斯药物股份有限公司 胰岛细胞制备性组合物和使用方法
EP3833365A4 (en) 2018-08-10 2022-05-11 Vertex Pharmaceuticals Incorporated Stem cell derived islet differentiation
US10724052B2 (en) 2018-09-07 2020-07-28 Crispr Therapeutics Ag Universal donor cells
TWI862513B (zh) * 2018-09-19 2024-11-21 日商千紙鶴治療公司 包含胰島素產生細胞之細胞集團、包含該細胞集團之醫藥、其用途及製造方法
CN113728090B (zh) * 2019-04-08 2024-04-05 诺和诺德股份有限公司 从干细胞衍生的定形内胚层生成胰腺内胚层
JP7555975B2 (ja) 2019-05-31 2024-09-25 ダブリュ．エル．ゴア アンド アソシエイツ，インコーポレイティド 生体適合性メンブレン複合体
WO2020243666A1 (en) 2019-05-31 2020-12-03 W. L. Gore & Associates, Inc. A biocompatible membrane composite
JP2022534545A (ja) 2019-05-31 2022-08-01 ダブリュ．エル．ゴア アンド アソシエイツ，インコーポレイティド 生体適合性メンブレン複合体
CA3257300A1 (en) 2019-05-31 2025-06-12 W. L. Gore & Associates, Inc. Cell encapsulation devices with controlled oxygen diffusion distances
US11118195B2 (en) 2019-09-05 2021-09-14 Crispr Therapeutics Ag Universal donor cells
AU2020340622A1 (en) 2019-09-05 2022-03-03 Crispr Therapeutics Ag Universal donor cells
KR20230146007A (ko) 2020-12-31 2023-10-18 크리스퍼 테라퓨틱스 아게 범용 공여자 세포
CN119432595A (zh) * 2023-08-07 2025-02-14 浙江霍德生物工程有限公司 利用生物反应器进行诱导多能干细胞的悬浮培养
WO2025117331A1 (en) * 2023-12-01 2025-06-05 Eli Lilly And Company Methods of making stem cell-derived islet-like cells, as well as populations and compositions including the same

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110280842A1 (en) * 2008-08-22 2011-11-17 Presidents And Fellows Of Harvard College Methods of reprogramming cells

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5843780A (en) 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
CN101240262A (zh) 2001-12-07 2008-08-13 杰龙公司 源自人胚胎干细胞的胰岛细胞
ES2564044T3 (es) 2003-06-27 2016-03-17 DePuy Synthes Products, Inc. Células posparto derivadas de tejido del cordón umbilical y métodos de preparación y uso de las mismas
US20050266554A1 (en) * 2004-04-27 2005-12-01 D Amour Kevin A PDX1 expressing endoderm
JP4819697B2 (ja) 2003-12-23 2011-11-24 ヴィアサイト，インコーポレイテッド 胚体内胚葉
KR101178786B1 (ko) 2004-03-23 2012-09-07 다이이찌 산쿄 가부시키가이샤 다능성 줄기세포의 증식 방법
JP2008538276A (ja) 2005-01-28 2008-10-23 ノヴァセラ・リミテッド 胚幹細胞培養のための方法
AU2006202209B2 (en) 2005-05-27 2011-04-14 Lifescan, Inc. Amniotic fluid derived cells
US8476070B2 (en) 2005-08-29 2013-07-02 Technion Research & Development Foundation Limited Media for culturing stem cells
EP3584311A1 (en) * 2005-10-27 2019-12-25 Viacyte, Inc. Pdx-1 expressing dorsal and ventral foregut endoderm
US7695965B2 (en) 2006-03-02 2010-04-13 Cythera, Inc. Methods of producing pancreatic hormones
DK2650359T3 (da) * 2006-03-02 2022-06-13 Viacyte Inc Endokrine prekursorceller, pancreatiske hormon-udtrykkende celler og fremstillingsfremgangsmåder
AU2007277364B2 (en) 2006-07-26 2010-08-12 Viacyte, Inc. Methods of producing pancreatic hormones
ES2704401T3 (es) 2006-08-02 2019-03-18 Technion Res & Dev Foundation Métodos de expansión de células madre embrionarias en un cultivo en suspensión
DK2173863T3 (en) 2007-06-29 2019-01-21 Fujifilm Cellular Dynamics Inc Automated method and apparatus for embryonic stem cell culture
KR101651661B1 (ko) * 2008-06-30 2016-08-26 얀센 바이오테크 인코포레이티드 만능 줄기 세포의 분화
CN102695790A (zh) 2009-10-29 2012-09-26 詹森生物科技公司 多能干细胞
WO2011139628A1 (en) * 2010-04-25 2011-11-10 Mount Sinai School Of Medicine Generation of anterior foregut endoderm from pluripotent cells
US9388386B2 (en) * 2011-12-22 2016-07-12 Janssen Biotech, Inc. Differentiation of human embryonic stem cells into single hormonal insulin positive cells
RU2684215C2 (ru) * 2012-12-31 2019-04-04 Янссен Байотек, Инк. Способ получения панкреатических эндокринных клеток (варианты) и способ увеличения выхода бета-клеток
US8859286B2 (en) 2013-03-14 2014-10-14 Viacyte, Inc. In vitro differentiation of pluripotent stem cells to pancreatic endoderm cells (PEC) and endocrine cells

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110280842A1 (en) * 2008-08-22 2011-11-17 Presidents And Fellows Of Harvard College Methods of reprogramming cells

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KR102700499B1 (ko) 2024-08-28
US20160215268A1 (en) 2016-07-28
EP3234109A4 (en) 2018-06-27
AU2015363008A1 (en) 2017-06-29
CA2970935A1 (en) 2016-06-23
KR102281752B1 (ko) 2021-07-23
WO2016100035A1 (en) 2016-06-23
BR112017012974A2 (pt) 2018-01-09
EP3234109A1 (en) 2017-10-25
JP2017537649A (ja) 2017-12-21
SG11201704961VA (en) 2017-07-28
AR103080A1 (es) 2017-04-12
TW201636421A (zh) 2016-10-16
KR20230050478A (ko) 2023-04-14
MX2017008176A (es) 2018-02-09
PH12017501153A1 (en) 2017-11-27
KR20210094141A (ko) 2021-07-28
KR20170087520A (ko) 2017-07-28
AU2015363008B2 (en) 2021-12-09
ZA201704868B (en) 2019-02-27
CN107250349A (zh) 2017-10-13
RU2017125728A3 (enExample) 2019-06-03
RU2017125728A (ru) 2019-01-22
JP6800854B2 (ja) 2020-12-16

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