KR102461687B1 - Cosmetic composition for scalp having antioxidant and anti-aging effect and method for preparing the same - Google Patents

Abstract

The present invention relates to a cosmetic composition for scalp having antioxidant and anti-aging effects, and a manufacturing method thereof. More specifically, the cosmetic composition for scalp comprising a persimmon leaf extract, hibiscus flower extract, Acacia concinna fruit extract, and malt extract of the present invention has excellent antioxidant and anti-inflammatory effects due to a synergistic effect of using the extracts together and can also show an effect on improving a condition of scalp, thereby having an anti-aging effect of delaying or alleviating aging of the scalp. In addition, the cosmetic composition for scalp of the present invention can be safely used because the composition contains the extracts obtained from natural raw materials.

Description

COSMETIC COMPOSITION FOR SCALP HAVING ANTIOXIDANT AND ANTI-AGING EFFECT AND METHOD FOR PREPARING THE SAME}

The present invention relates to a cosmetic composition for the scalp having antioxidant and anti-aging effects and a method for preparing the same.

The scalp is a skin tissue that protects the head, and its essential role is to maintain and protect the inside of the head by buffering physical or chemical stimuli from the outside. occupies In other words, problems that occur continuously on the scalp, such as inflammatory scalp and hair loss, aggravate depression or anxiety symptoms, increase the perception of stress, and cause difficulties in human or heterosexual relationships, which in turn drastically reduce the quality of life. develops to

Various causes have been pointed out as the causes of problems on the scalp, such as physical and chemical stimulation applied to the scalp due to hair dyes and perming agents for beauty, environmental pollution such as fine dust, stress, and changes in eating habits. Problems such as , stinging, and inflammation often lead to hair loss.

As such, when there is a problem with the scalp, if you look at the condition of the scalp by using a device to enlarge it, the pores lose elasticity and sagging, that is, the aging of the scalp is in many cases, and various wastes and sebum are in the enlarged pores. It builds up and causes inflammation, causing redness and itching.

The aging of the scalp is less sensitive than the face and can be recognized only after trouble occurs. In particular, since there is a problem that the scalp, which is the starting point of the skin, sags and the facial line collapses. do.

On the other hand, conventional methods of using natural extracts to protect or improve the condition of the scalp, or to alleviate hair loss, etc. have been devised.

For example, Korea Patent Publication No. 10-2018-0111015 discloses a cosmetic composition for preventing hair loss or promoting hair growth containing lavender, lemongrass, rosemary, chamomile and peppermint oil as active ingredients. In addition, Korean Patent Application Laid-Open No. 10-2014-0139930 discloses a shampoo composition for improving scalp and hair containing natural extracts of oriental medicine such as gold as an active ingredient.

However, the above techniques have a problem that the intended effect may not be properly exhibited because the process is complicated or the active ingredient contained in the raw material is denatured or destroyed in the process of preparing the extract using high temperature and high pressure in the extraction process.

Therefore, there is a need to develop a cosmetic composition for the scalp that can exhibit excellent antioxidant and anti-aging effects when applied to the scalp while using natural materials that do not have safety problems when applied to the human body.

Korean Patent Publication No. 10-2018-0111015 Korean Patent Publication No. 10-2014-0139930

The present inventors recognized the problems of the prior art, and confirmed that a composition for scalp comprising a persimmon leaf extract, a hibiscus flower extract, a shikakai fruit extract and a malt extract exhibits excellent antioxidant and anti-aging effects, and the present invention came to completion.

The present invention relates to a cosmetic composition for the scalp comprising a persimmon leaf extract, a hibiscus flower extract, a shikakai fruit extract and a malt extract.

The present invention relates to a method for preparing the cosmetic composition for the scalp.

The present invention relates to a method of applying the cosmetic composition for the scalp to the scalp.

Hereinafter, this will be described in detail. All combinations of the various elements disclosed herein are within the scope of the present invention. In addition, it cannot be considered that the scope of the present invention is limited by the following specific description.

In addition, unless otherwise defined in this specification, all terms used in the specification should be understood to have the same meaning as commonly understood by one of ordinary skill in the art to which the present invention belongs.

Also, the singular expression includes the plural expression unless the context clearly dictates otherwise.

In this specification, terms such as first, second, etc. may be used to distinguish or describe one component from another component, but the components should not be construed as being limited by the terms. For example, within the scope of the present invention, a first component may be termed a second component or vice versa.

In addition, in this specification, "degree" combined with a number represents degrees Celsius (℃), and may be used interchangeably with "℃" (for example, "100 degrees" has the same meaning as "100 degrees Celsius") used as).

The present invention provides a cosmetic composition for the scalp comprising a persimmon leaf extract, a hibiscus flower extract, a shikakai fruit extract and a malt extract.

In the present invention, "persimmon leaf" refers to the leaf part of the persimmon tree, and the persimmon tree (Diospyros kaki) is a broad-leaved arboreous tree, a fruit tree widely cultivated in northern central China, Japan, and south central Korea as a special species of the temperate zone in East Asia. The persimmon leaves contain a large amount of ascorbic acid, which has antioxidant and whitening effects. It is reported that there is

In the present invention, "hibiscus flower" means the flower part of hibiscus, and the hibiscus is a generic term for plants belonging to the genus Mallow and Mugunghwa, a dicotyledonous plant. means The hibiscus is known to have a diuretic, antihypertensive, and antibacterial action because it contains active ingredients useful for the human body, and is known to implement skin whitening and moisturizing effects.

In the present invention, "shikakai fruit" means the fruit part of shikakai, and the shikakai (Acacia concinna) maintains sufficient moisture in the hair to the extent that it has the meaning of a tree fruit for hair, and makes it clean and shiny. It is known as a natural plant that helps to eradicate bacteria and eliminate itching by acting as an antiviral and disinfectant.

In the present invention, "malt (Hordei Fructus Germinatus)" refers to a medicinal material made by taking sprouts from the rice family, drying them, and then lightly roasting them. When the malt sprouts, an enzyme that converts starch in the seeds into sugar is produced to give the malt sweetness, which is used to make sikhye, syrup, and red pepper paste. Malt contains diastase, invertase, B vitamins, fat, phospholipids, and the like.

In the present invention, "extract" means the result of extraction using an appropriate extraction solvent, and the extract is an extract obtained by extraction, a diluted or concentrated solution of the extract, a dried product obtained by drying the extract, a prepared or purified product thereof, Or fractions, or mixtures thereof, including extracts of all formulations that can be formed using the extract itself and the extract.

The extract of the present invention may be prepared and used in the form of a dry powder after extraction, but is not limited thereto.

In the present invention, the extract may be prepared through an extraction method commonly used in the art. For example, methods such as hot water extraction, hot water extraction, cold extraction, reflux cooling extraction, ultrasonic extraction, high-temperature pressure extraction, and the like may be used.

In preparing the extract in the present invention, water as an extraction solvent; lower alcohols having 1 to 4 carbon atoms, such as methanol, ethanol, propyl alcohol, and butyl alcohol; polyhydric alcohols such as glycerin, butylene glycol and propylene glycol; hydrocarbon solvents such as chloroform, methyl acetate, ethyl acetate, benzene, hexane, diethyl ether, petroleum ether and dichloromethane; Or a mixture thereof may be used, but is not limited thereto.

The persimmon leaf extract and/or hibiscus flower extract included in the composition of the present invention may be prepared by the following method.

S1-1) drying and pulverizing persimmon leaves and/or hibiscus flowers, and adding ethanol;

S1-2) separating the residue by filtration after leaving the pulverized product at a temperature of 20 to 24;

S1-3) adding purified water to the separated residue;

S1-4) extracting the residue at a temperature of 30 to 60 degrees for 24 to 72 hours; and

S1-5) filtering the extract, and aging at a temperature of 4 to 10 degrees for 5 to 10 days.

Steps S1-1 and S1-2 include adding a primary solvent to the raw material, leaving the raw material mixed in the solvent, filtering and removing the active ingredient extracted with the primary solvent, and separating the residue to obtain to be.

In the step S1-1, the particle size of the raw material after pulverization is not limited, but the smaller it may be, the more advantageous it is to increase the effect of the extract prepared through the subsequent step.

In steps S1-1 and S1-2, ethanol is used as the primary solvent, and according to embodiments of the present invention, it may be 50 to 90% (v/v) of ethanol, preferably 80% ( v/v) may be ethanol, but is not limited thereto.

Step S1-2 may be performed at a temperature of 15 to 70 degrees, and according to embodiments of the present invention, may be performed at a temperature of 20 to 24 degrees.

If it is carried out below the temperature range, the effect to be achieved through step S1-2 in the present invention may not be sufficient because the extraction of the component to be removed is not sufficient, and when it is performed beyond the temperature range, the removal In addition to the desired component, other components may be excessively eluted from the raw material, and the active component to be utilized may be destroyed or denatured, so that the intended effect of the present invention may not be sufficiently exhibited.

Step S1-2 may be performed for 3 to 10 hours.

When it is performed less than the time range, the effect to be achieved through step S1-2 in the present invention may not be sufficient because the extraction of the component to be removed is not sufficient, and when it is performed outside the time range, the removal In addition to the desired component, other components may be excessively eluted from the raw material, and the active component to be utilized may be destroyed or denatured, so that the intended effect of the present invention may not be sufficiently exhibited.

Steps S1-3 and S1-4 are steps of adding a secondary solvent to the residue separated in steps S1-1 and S1-2, and extracting the active ingredient from the raw material.

In steps S1-3 and S1-4, purified water is used as a secondary solvent, and is used as a solvent for extracting the active ingredient substantially used in the present invention from raw materials such as persimmon leaves and hibiscus flowers.

Step S1-4 may be performed at a temperature of 30 to 60 degrees for 24 to 72 hours.

When it is carried out below the temperature range, the extraction of the active ingredient is not sufficient and the intended effect in the present invention may not be sufficiently exhibited, and when it is carried out above the temperature range, the active ingredient may be destroyed or denatured.

In addition, when it is carried out less than the time range, the extraction of the active ingredient is not sufficient and the intended effect in the present invention may not be sufficiently exhibited. Since the extraction rate of the active ingredient is not high compared to time, it may not be suitable for manufacturing efficiency.

The step S1-5 is a step of aging the extract obtained in step S1-4, and is performed to increase the antioxidant and anti-aging effect intended by the present invention due to the extract.

Step S1-5 may be performed for 5 to 10 days at a temperature of 4 to 10 degrees, but is not limited thereto.

The Shikakai fruit extract and/or malt extract included in the composition of the present invention may be prepared by the following method.

S2-1) pulverizing Shikakai fruit and/or malt and adding ethanol;

S2-2) extracting the pulverized product at a temperature of 20 to 24 for 24 to 72 hours; and

S2-3) filtering the extract, and aging at a temperature of 4 to 10 degrees for 5 to 10 days.

Step S2-1 is a step of pulverizing the raw material and adding a solvent.

According to embodiments of the present invention, ethanol is used as a solvent in step S2-1, and according to embodiments of the present invention, it may be 50 to 90% (v/v) of ethanol, preferably It may be 80% (v/v) ethanol, but is not limited thereto.

Step S2-2 is a step of extracting the active ingredient from the raw material mixed with the solvent after grinding in step S2-1.

Step S2-2 may be performed at a temperature of 20 to 24 degrees for 24 to 72 hours.

When it is carried out below the temperature range, the extraction of the active ingredient is not sufficient, so the intended effect may not be sufficiently exhibited in the present invention, and when it is carried out above the temperature range, the active ingredient may be destroyed or denatured .

In addition, when it is carried out less than the time range, the extraction of the active ingredient is not sufficient, so the intended effect may not be sufficiently exhibited in the present invention, and when it is performed beyond the time range, the active ingredient is destroyed or denatured or , it may not be suitable for manufacturing efficiency because the extraction rate of the active ingredient is not high compared to the time.

The step S2-3 is a step of aging the extract obtained in step S2-2, and is performed to increase the antioxidant and anti-aging effects intended by the present invention due to the extract.

Step S2-3 may be performed for 5 to 10 days at a temperature of 4 to 10 degrees, but is not limited thereto.

In the cosmetic composition of the present invention, the persimmon leaf extract, hibiscus flower extract, shikakai fruit extract and malt extract contained in the composition may be included in a specific content or a specific weight ratio.

According to embodiments of the present invention, the composition comprises 0.1 to 10 parts by weight of persimmon leaf extract, 0.1 to 5 parts by weight of hibiscus flower extract, 0.1 to 5 parts by weight of Shikakai fruit extract, and 0.1 to 5 parts by weight of malt extract. However, the present invention is not limited thereto.

In addition, according to embodiments of the present invention, the composition may include persimmon leaf extract, hibiscus flower extract, shikakai fruit extract and malt extract in a weight ratio of 2:1:1:1, but is limited thereto. not.

In the experimental examples described below, when persimmon leaf extract, hibiscus flower extract, shikakai fruit extract and malt extract are included in a specific content or a specific weight ratio in the cosmetic composition for the scalp, antioxidant and anti-aging effects due to the synergistic effect of the extracts In particular, it has been specifically demonstrated that it can be excellent.

In the present invention, the cosmetic composition may exhibit antioxidant and anti-inflammatory effects, and thus may have anti-aging effects.

In the present invention, "antioxidation" means to prevent oxidation and aging of other cells or tissues by removing them with active oxygen.

In the present invention, "anti-inflammatory" refers to an inflammatory process or a property to inhibit it.

The components included in the cosmetic composition of the present invention may include components commonly used in the cosmetic composition, for example, conventional auxiliary agents such as antioxidants, stabilizers, solubilizers, vitamins, pigments and fragrances, and carriers. .

The carrier includes purified water, oil, wax, fatty acid, fatty alcohol, fatty acid ester, emulsifier (xanthan gum, ceteareth-6 olivate, etc.), moisture absorbent, thickener (xanthan gum, magnesium aluminum silicate, etc.), viscosity stabilizer, kill A rating agent, a buffering agent, a preservative (1,2-hexanediol, etc.), a moisturizing agent (butylene glycol, etc.), lower alcohol, etc. may be used without limitation, but is not limited thereto.

In addition, an anti-inflammatory agent, an antibacterial agent, an antifungal agent, a sunscreen agent, an antibiotic, etc. may be used in the composition of the present invention, if necessary.

The cosmetic composition of the present invention is, for example, a solution, suspension, emulsion, paste, gel, powder, cream, lotion, lotion, water-soluble liquid, essence, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation , spray, shampoo, rinse, body wash, soap, etc. may be formulated in the formulation, and according to embodiments of the present invention, it may be formulated in the shampoo type, but is not limited thereto, and in the cosmetic industry It may be prepared in any form of formulation that is conventionally prepared.

When the cosmetic composition of the present invention is formulated as a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide as a carrier component and the like may be used, but is not limited thereto.

When the cosmetic composition of the present invention is formulated as a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additional chlorofluoro propellants such as, but not limited to, hydrocarbons, propane/butane or dimethyl ether.

When the cosmetic composition of the present invention is formulated as a solution or emulsion, a solvent, solubilizer or emulsifier is used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate. , propylene glycol, 1,3-butyl glycol oil, glycerol fatty ester, polyethylene glycol or fatty acid ester of sorbitan, but is not limited thereto.

When the cosmetic composition of the present invention is formulated as a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester, such as suspension agent, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracanth may be used, but is not limited thereto.

When the cosmetic composition of the present invention is formulated with surfactant-containing cleansing, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarco Cinate, fatty acid amide ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative or ethoxylated glycerol fatty acid ester may be used, but is not limited thereto.

Other ingredients other than the above-mentioned various moisturizing ingredients can be appropriately selected and formulated by those skilled in the art without difficulty depending on the formulation or purpose of use.

Another aspect of the present invention provides a method for producing a cosmetic composition for the scalp comprising the following steps.

S3-1) preparing persimmon leaf extract, hibiscus flower extract, shikakai fruit extract and malt extract, respectively; and

S3-2) mixing the prepared extract.

In the manufacturing method, the persimmon leaf extract or hibiscus flower extract may be prepared by a method comprising the following steps.

S1-1) drying and pulverizing persimmon leaves or hibiscus flowers, and adding ethanol;

S1-2) separating the residue by filtration after leaving the pulverized product at a temperature of 20 to 24 degrees;

S1-3) adding purified water to the separated residue;

S1-4) extracting the residue at a temperature of 30 to 60 degrees for 24 to 72 hours; and

S1-5) filtering the extract, and aging at a temperature of 4 to 10 degrees for 5 to 10 days.

In addition, in the preparation method, the Shikakai fruit extract or malt extract may be prepared by a method comprising the following steps.

S2-1) pulverizing Shikakai fruit or malt and adding ethanol;

S2-2) extracting the pulverized product at a temperature of 20 to 24 degrees for 24 to 72 hours; and

S2-3) filtering the extract, and aging at a temperature of 4 to 10 degrees for 5 to 10 days.

Another aspect of the present invention provides a method for applying the cosmetic composition to the scalp comprising the steps of:

applying the cosmetic composition to the scalp as a whole; and

Massage for 5 to 10 minutes so that the cosmetic composition is absorbed into the scalp.

In the manufacturing method of the cosmetic composition and the scalp application method of the present specification, specific details are the same as those described in the cosmetic composition unless otherwise stated, and are omitted to avoid excessive repetition of the same content in the specification.

The cosmetic composition for scalp comprising the persimmon leaf extract, hibiscus flower extract, shikakai fruit extract and malt extract of the present invention has excellent antioxidant and anti-inflammatory effects due to the synergistic effect of using the extracts together, and improves scalp condition Because it can also exhibit an effect, it has an anti-aging effect that delays or renews aging of the scalp.

In addition, since the cosmetic composition for the scalp of the present invention contains an extract obtained from a natural raw material, it can be safely used.

1 shows the results for dose-dependent cytotoxicity of the composition of the present invention.

Hereinafter, the present invention will be described in more detail by way of Examples. However, these examples are for illustrative purposes only, and the scope of the present invention is not limited by these examples. In addition, it should be understood that terms not specifically defined in this specification have meanings commonly used in the technical field to which the present invention pertains.

Preparation Example 1. Preparation of persimmon leaf extract and hibiscus flower extract (1)

Persimmon leaves and hibiscus flowers (purchased from the open market) were dried and pulverized, placed in separate containers, and then 5 times the volume of 80% (v/v) ethanol was added.

Then, the residue was separated by filtration through filter paper after leaving it to stand for about 6 hours while maintaining the temperature of 20 to 24.

After putting the separated residue in a container, 10 times the volume of purified water was added thereto, and extraction was performed at a temperature of about 40°C for about 2 days.

Thereafter, the extract was filtered through filter paper, and aged at a temperature of 4 to 10 degrees for one week to prepare a persimmon leaf extract and a hibiscus flower extract.

Preparation Example 2. Preparation of persimmon leaf extract and hibiscus flower extract (2)

Persimmon leaves and hibiscus flowers (purchased from the open market) were dried and pulverized, each put in a container, 10 times the volume of purified water was added, and extracted at a temperature of 30 to 60 degrees for about 2 days.

Thereafter, the extract was filtered through filter paper, and aged at a temperature of 4 to 10 degrees for one week to prepare a persimmon leaf extract and a hibiscus flower extract.

Preparation Example 3. Preparation of Shikakai Fruit Extract and Malt Extract

Shikakai fruit and malt powder (purchased from the open market) prepared by drying and pulverizing were placed in each container, 10 times the volume of 80% (v/v) ethanol was added, and then extracted at a temperature of 20 to 24 for about 2 days. .

Thereafter, the extract was filtered through filter paper and aged at a temperature of 4 to 10 degrees for one week to prepare a Shikakai fruit extract and a malt extract.

Preparation of Examples 1 to 9

The extracts prepared in Preparation Example 1 and Preparation Example 3 were prepared by mixing according to the following weight ratio.

Persimmon Leaf Extract Hibiscus Flower Extract Shikakai fruit extract malt extract Example 1 One One One One Example 2 2 One One One Example 3 One 2 One One Example 4 One One 2 One Example 5 One One One 2 Example 6 2 2 One One Example 7 One 2 2 One Example 8 One One 2 2 Example 9 2 One One 2

Preparation of Comparative Examples 1 and 2

It was prepared by mixing the extracts prepared in Preparation Examples 2 and 3 according to the following weight ratio.

persimmon leaf
extract hibiscus flower
extract Shikakai fruit
extract malt
extract Comparative Example 1 One One One One Comparative Example 2 2 One One One

Experimental example 1. Check for cytotoxicity

The dose-dependent cytotoxicity of the composition of the present invention was confirmed by MTT assay.

에서 5% CO₂ 인큐베이터로 24시간 동안 배양하였다.Specifically, the Raw 264.7 cell line was inoculated into a 12-well plate at 1Х10 ⁴ cells/ml, and 37 in DMEM medium.

5% CO ₂ Incubated in an incubator for 24 hours.

After culturing for 24 hours, the medium was replaced with a fresh medium containing the composition of Example 1, and cultured again for 40 hours.

Thereafter, the cells were washed and incubated in a medium containing 0.5 mg/mL MTT solution for 4 hours.

Finally, the medium was replaced with 200 μL DMSO, and the absorbance was measured at 570 nm to examine the cell viability, and the results are shown in FIG. 1 .

As can be seen in FIG. 1 , when the composition of the present invention was treated, it had little effect on the survival rate according to the dose change, thereby confirming the safety of the composition of the present invention.

Experimental Example 2. Confirmation of antioxidant effect

Antioxidant activity was confirmed through DPPH radical scavenging activity.

Specifically, 50 μl of a DPPH solution was added to 50 μg/ml of samples of Examples and Comparative Examples, and 1 mg/ml of methanol as a control, and mixed by stirring for 10 seconds. Then, the mixed solution was placed in a 12-well plate and left for about 30 minutes. After measuring the absorbance at 517 nm using a UV spectrophotometer, the average value was calculated.

DPPH radical scavenging activity was calculated according to Equation 1 below, and the experimental results are shown in Table 3 below.

[Equation 1]

DPPH radical scavenging activity (%) = [(control absorbance - absorbance of sample sample)/(control absorbance)] x 100

DPPH radical scavenging activity (%) Example 1 67 Example 2 Example 3 67 Example 4 70 Example 5 74 Example 6 75 Example 7 Example 8 74 Example 9 Comparative Example 1 52 Comparative Example 2 55

As shown in the above table, it can be confirmed that the cosmetic composition of the present invention exhibits high DPPH scavenging activity.

In addition, in the case of Example 2, in which persimmon leaf extract, hibiscus flower extract, shikakai fruit extract and malt extract were mixed in a weight ratio of 2:1:1:1, it showed better DPPH scavenging activity compared to other examples. that can be checked

On the other hand, in the case of Comparative Examples 1 and 2, which were carried out with some changes in the manufacturing method of the persimmon leaf extract and the hibiscus flower extract, the DPPH scavenging activity was slightly lower than that of the Example, and the DPPH scavenging activity could be increased even by the difference in the manufacturing method. can confirm.

Experimental Example 3. Confirmation of anti-inflammatory effect

To confirm the anti-inflammatory effect, NO scavenging ability was measured by Griess reagent assay.

에서 5% CO₂ 인큐베이터로 24시간 동안 배양하였다.Specifically, the Raw 264.7 cell line was inoculated into a 12-well plate at 5Х10 ⁴ cells/ml, and 37 in DMEM medium containing 10% FBS, 100 U/mL penicillin and 100 μg/mL streptomycin.

5% CO ₂ Incubated in an incubator for 24 hours.

Then, after treatment with 1 μg/ml of LPS and left for 1 hour, 50 μg/ml of the compositions of Examples and Comparative Examples prepared above were treated, and cultured for 24 hours.

하여 상층액을 수득한 후 96-웰 플레이트로 옮기고, griess 시약과 반응 시킨 후 ELISA reader로 540 nm에서 흡광도를 측정하여 NO 생성율을 하기 표 4에 나타내었다.Finally, the medium was centrifuged (3,000 rpm, 10 min, 4

to obtain a supernatant, transferred to a 96-well plate, reacted with griess reagent, and then measured for absorbance at 540 nm with an ELISA reader, and the NO production rate is shown in Table 4 below.

NO production rate (%) Example 1 42 Example 2 25 Example 3 Example 4 44 Example 5 37 Example 6 35 Example 7 52 Example 8 34 Example 9 Comparative Example 1 63 Comparative Example 2

As shown in the above table, it can be confirmed that the cosmetic composition of the present invention has an activity of inhibiting NO production.

In addition, in the case of Example 2, in which the persimmon leaf extract, hibiscus flower extract, shikakai fruit extract and malt extract were mixed in a weight ratio of 2:1:1:1, it was a more excellent NO production in comparison with other examples. It can be seen that the activity is shown.

On the other hand, in the case of Comparative Examples 1 and 2, which were carried out with some changes in the manufacturing method of the persimmon leaf extract and the hibiscus flower extract, it showed a somewhat lower activity of inhibiting NO generation compared to the Example, thereby inhibiting NO generation even by the difference in the manufacturing method. It can be confirmed that the activity can be increased.

Experimental Example 4. Evaluation of usability

To evaluate the feeling of use, a test was conducted for 7 days on 40 adult males.

Specifically, the composition of Example 2 was used for the experiment, and 6 g of polyquaternium-44 1.8 g, xanthan gum 0.3 g, glucamate (PEG-120 Methyl Glucose Dioleate) 6 g, decyl glucoside 50 g, cocoa Betaine 20 g, Olive Liquid (Olive PEG-7 Esters) 2 g, Olive Oil 2 g, Vegetable Glycerin 4 g, Peppermint Essential Oil 0.5 g, Rosemary Essential Oil 0.5 g, Menthol 0.2 g, Silk Amino Acid 0.3 g, Marine Elastin 0.5 g, 0.5 g of marine collagen was additionally mixed, and the mixture of Example 2 was prepared as a shampoo formulation in a commonly used method so as to be included in 12.5 parts by weight based on the total weight of the shampoo, and used in the experiment.

는 샴푸 조성물에 대한 만족도가 높음을, β는 샴푸 조성물에 대한 만족도가 보통임을, Х는 샴푸 조성물에 대한 만족도가 낮음을 의미한다.In Table 5 below

indicates high satisfaction with the shampoo composition, β indicates moderate satisfaction with the shampoo composition, and Х indicates low satisfaction with the shampoo composition.

detergency Soft hair tangling relief improvement of scalp hardness Example 2 O O △ O

As can be seen in Table 5, the cosmetic composition for the scalp of the present invention received good evaluations in terms of cleaning power, softness, hair tangling relief, and scalp hardness improvement.

In particular, with respect to the phenomenon of hardening of the scalp, which is often seen in the aged scalp, it can be confirmed that the scalp hardness is improved when the composition of the present invention is applied to the scalp, so that the composition of the present invention is useful for anti-aging of the scalp It was confirmed that it can be used.

Claims (5)

A cosmetic composition for scalp comprising a persimmon leaf extract, a hibiscus flower extract, a shikakai fruit extract and a malt extract. According to claim 1,
The persimmon leaf extract and hibiscus flower extract are cosmetic compositions for the scalp prepared by the following method:
S1-1) drying and pulverizing persimmon leaves or hibiscus flowers, and adding ethanol;
S1-2) separating the residue by filtration after leaving the pulverized product at a temperature of 20 to 24° C. for 3 to 10 hours;
S1-3) adding purified water to the separated residue;
S1-4) extracting the residue at a temperature of 30 to 60° C. for 24 to 72 hours; and
S1-5) filtering the extract, and aging for 5 to 10 days at a temperature of 4 to 10 ℃. According to claim 1,
The shikakai fruit extract and malt extract is a cosmetic composition for the scalp prepared by the following method:
S2-1) pulverizing Shikakai fruit or malt and adding ethanol;
S2-2) extracting the pulverized product at a temperature of 20 to 24° C. for 24 to 72 hours; and
S2-3) filtering the extract, and aging for 5 to 10 days at a temperature of 4 to 10 ℃. According to claim 1,
The composition is a cosmetic composition for the scalp comprising a persimmon leaf extract, a hibiscus flower extract, a shikakai fruit extract and a malt extract in a weight ratio of 2:1:1:1. According to claim 1,
The composition is a cosmetic composition for the scalp having antioxidant and anti-inflammatory effects.

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