KR102439719B1 - 글루타민 합성효소 유전자내 상보성 벡터를 사용한 높은 수준의 헤테로머 단백질을 발현하는 세포의 직접적인 선택 - Google Patents

글루타민 합성효소 유전자내 상보성 벡터를 사용한 높은 수준의 헤테로머 단백질을 발현하는 세포의 직접적인 선택 Download PDF

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KR102439719B1
KR102439719B1 KR1020217035841A KR20217035841A KR102439719B1 KR 102439719 B1 KR102439719 B1 KR 102439719B1 KR 1020217035841 A KR1020217035841 A KR 1020217035841A KR 20217035841 A KR20217035841 A KR 20217035841A KR 102439719 B1 KR102439719 B1 KR 102439719B1
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랜덜 알. 켓쳄
제프리 티. 맥그류
얘들린 디나 에이. 포미나
트렌트 피. 문로
니르야 자그디쉬 아그라왈
크리스틴 엠. 다리스
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
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    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
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    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
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    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
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    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y104/00—Oxidoreductases acting on the CH-NH2 group of donors (1.4)
    • C12Y104/01—Oxidoreductases acting on the CH-NH2 group of donors (1.4) with NAD+ or NADP+ as acceptor (1.4.1)
    • C12Y104/01002—Glutamate dehydrogenase (1.4.1.2)
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    • C12Y—ENZYMES
    • C12Y603/00—Ligases forming carbon-nitrogen bonds (6.3)
    • C12Y603/01—Acid-ammonia (or amine)ligases (amide synthases)(6.3.1)
    • C12Y603/01002—Glutamate-ammonia ligase (6.3.1.2)
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    • C12Y603/00—Ligases forming carbon-nitrogen bonds (6.3)
    • C12Y603/04—Other carbon-nitrogen ligases (6.3.4)
    • C12Y603/04004—Adenylosuccinate synthase (6.3.4.4)
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    • C12Y603/00—Ligases forming carbon-nitrogen bonds (6.3)
    • C12Y603/04—Other carbon-nitrogen ligases (6.3.4)
    • C12Y603/04005—Argininosuccinate synthase (6.3.4.5)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • C12N2015/8518—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic expressing industrially exogenous proteins, e.g. for pharmaceutical use, human insulin, blood factors, immunoglobulins, pseudoparticles

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  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
KR1020217035841A 2016-05-11 2017-05-11 글루타민 합성효소 유전자내 상보성 벡터를 사용한 높은 수준의 헤테로머 단백질을 발현하는 세포의 직접적인 선택 Active KR102439719B1 (ko)

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US201662334966P 2016-05-11 2016-05-11
US62/334,966 2016-05-11
KR1020187035185A KR102323519B1 (ko) 2016-05-11 2017-05-11 글루타민 합성효소 유전자내 상보성 벡터를 사용한 높은 수준의 헤테로머 단백질을 발현하는 세포의 직접적인 선택
PCT/US2017/032139 WO2017197098A1 (en) 2016-05-11 2017-05-11 Direct selection of cells expressing high levels of heteromeric proteins using glutamine synthetase intragenic complementation vectors

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KR102439719B1 true KR102439719B1 (ko) 2022-09-02

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KR1020217035841A Active KR102439719B1 (ko) 2016-05-11 2017-05-11 글루타민 합성효소 유전자내 상보성 벡터를 사용한 높은 수준의 헤테로머 단백질을 발현하는 세포의 직접적인 선택

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CN109988777A (zh) * 2017-12-29 2019-07-09 南京金斯瑞生物科技有限公司 谷氨酰胺合成酶基因以及应用
CA3137457A1 (en) * 2019-05-07 2020-11-12 Amgen Inc. Vectors and expression systems for producing recombinant proteins
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WO2023125389A1 (en) * 2021-12-27 2023-07-06 Shanghai Zhenge Biotechnology Co., Ltd. Novel markers for recombinant production system
CN118900915A (zh) 2022-03-23 2024-11-05 勃林格殷格翰国际公司 作为哺乳动物细胞中的选择标记物的细菌谷氨酰胺合成酶
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WO2025037708A1 (en) * 2023-08-16 2025-02-20 Korea Advanced Institute Of Science And Technology Method for selection of cell line expressing high levels of recombinant protein using glutamine synthetase split expression vector
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IL300591A (en) 2023-04-01
KR102323519B1 (ko) 2021-11-09
EP3455359A1 (en) 2019-03-20
TW202223093A (zh) 2022-06-16
CN115873904A (zh) 2023-03-31
AU2017263454A1 (en) 2018-12-06
CA3024027A1 (en) 2017-11-16
MX394682B (es) 2025-03-24
IL262923B1 (en) 2023-04-01
BR112018073316A2 (pt) 2019-06-04
CN109219660A (zh) 2019-01-15
AU2023200660A1 (en) 2023-03-09
EP3455359B1 (en) 2022-03-09
US20190127452A1 (en) 2019-05-02
KR20210135635A (ko) 2021-11-15
TW201805422A (zh) 2018-02-16
MX2022009674A (es) 2022-09-09
TWI821905B (zh) 2023-11-11
KR20190005193A (ko) 2019-01-15
US11384140B2 (en) 2022-07-12
MX2018013882A (es) 2019-03-14
AU2017263454B2 (en) 2023-02-09
IL262923B2 (en) 2023-08-01
WO2017197098A1 (en) 2017-11-16
TWI757291B (zh) 2022-03-11
SG11201810040WA (en) 2018-12-28
JP7344949B2 (ja) 2023-09-14
AU2023200660B2 (en) 2024-12-12
MA44976A (fr) 2019-03-20
IL262923A (en) 2019-01-31
CN109219660B (zh) 2023-01-06
US20230048658A1 (en) 2023-02-16
CL2018003210A1 (es) 2019-02-15
JP2022033734A (ja) 2022-03-02
EP4067493A1 (en) 2022-10-05
ES2914118T3 (es) 2022-06-07
EA201892588A1 (ru) 2019-06-28
JP2019514417A (ja) 2019-06-06
JP6972022B2 (ja) 2021-11-24

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