KR102323519B1 - 글루타민 합성효소 유전자내 상보성 벡터를 사용한 높은 수준의 헤테로머 단백질을 발현하는 세포의 직접적인 선택 - Google Patents

글루타민 합성효소 유전자내 상보성 벡터를 사용한 높은 수준의 헤테로머 단백질을 발현하는 세포의 직접적인 선택 Download PDF

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KR102323519B1
KR102323519B1 KR1020187035185A KR20187035185A KR102323519B1 KR 102323519 B1 KR102323519 B1 KR 102323519B1 KR 1020187035185 A KR1020187035185 A KR 1020187035185A KR 20187035185 A KR20187035185 A KR 20187035185A KR 102323519 B1 KR102323519 B1 KR 102323519B1
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랜덜 알. 켓쳄
제프리 티. 맥그류
얘들린 디나 에이. 포미나
트렌트 피. 문로
니르야 자그디쉬 아그라왈
크리스틴 엠. 다리스
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암젠 인크
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
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    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
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    • C12Y104/00—Oxidoreductases acting on the CH-NH2 group of donors (1.4)
    • C12Y104/01—Oxidoreductases acting on the CH-NH2 group of donors (1.4) with NAD+ or NADP+ as acceptor (1.4.1)
    • C12Y104/01002—Glutamate dehydrogenase (1.4.1.2)
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    • C12Y—ENZYMES
    • C12Y603/00—Ligases forming carbon-nitrogen bonds (6.3)
    • C12Y603/01—Acid-ammonia (or amine)ligases (amide synthases)(6.3.1)
    • C12Y603/01002—Glutamate-ammonia ligase (6.3.1.2)
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    • C12Y603/04004—Adenylosuccinate synthase (6.3.4.4)
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    • C12Y603/04—Other carbon-nitrogen ligases (6.3.4)
    • C12Y603/04005—Argininosuccinate synthase (6.3.4.5)
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    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • C12N2015/8518—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic expressing industrially exogenous proteins, e.g. for pharmaceutical use, human insulin, blood factors, immunoglobulins, pseudoparticles

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  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
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  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
KR1020187035185A 2016-05-11 2017-05-11 글루타민 합성효소 유전자내 상보성 벡터를 사용한 높은 수준의 헤테로머 단백질을 발현하는 세포의 직접적인 선택 Expired - Fee Related KR102323519B1 (ko)

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KR1020217035841A KR102439719B1 (ko) 2016-05-11 2017-05-11 글루타민 합성효소 유전자내 상보성 벡터를 사용한 높은 수준의 헤테로머 단백질을 발현하는 세포의 직접적인 선택

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US201662334966P 2016-05-11 2016-05-11
US62/334,966 2016-05-11
PCT/US2017/032139 WO2017197098A1 (en) 2016-05-11 2017-05-11 Direct selection of cells expressing high levels of heteromeric proteins using glutamine synthetase intragenic complementation vectors

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KR1020217035841A Active KR102439719B1 (ko) 2016-05-11 2017-05-11 글루타민 합성효소 유전자내 상보성 벡터를 사용한 높은 수준의 헤테로머 단백질을 발현하는 세포의 직접적인 선택

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BR112019024750A2 (pt) 2017-05-24 2020-06-09 Thoeris Gmbh uso de glutamina sintetase no tratamento da hiperamonemia
CN109988777A (zh) * 2017-12-29 2019-07-09 南京金斯瑞生物科技有限公司 谷氨酰胺合成酶基因以及应用
SG11202109642SA (en) * 2019-05-07 2021-10-28 Amgen Inc Vectors and expression systems for producing recombinant proteins
WO2023011442A1 (en) * 2021-08-03 2023-02-09 Shanghai Zhenge Biotechnology Co., Ltd. Selectable markers for eukaryotic expression system
CN118804978A (zh) * 2021-12-27 2024-10-18 上海臻格生物技术有限公司 用于重组生产系统的新型标志物
EP4496884A1 (en) * 2022-03-23 2025-01-29 Boehringer Ingelheim International Gmbh New glutamine synthetase variants as selection marker
AU2023238775A1 (en) 2022-03-23 2024-10-31 Boehringer Ingelheim International Gmbh Bacterial glutamine synthetase as selection marker in mammalian cells
WO2025037708A1 (en) * 2023-08-16 2025-02-20 Korea Advanced Institute Of Science And Technology Method for selection of cell line expressing high levels of recombinant protein using glutamine synthetase split expression vector
WO2025128343A1 (en) 2023-12-11 2025-06-19 Just-Evotec Biologics, Inc. Protein expression using trans-splicing and split selectable markers
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CN109219660A (zh) 2019-01-15
TW202223093A (zh) 2022-06-16
KR102439719B1 (ko) 2022-09-02
EP4067493A1 (en) 2022-10-05
JP2019514417A (ja) 2019-06-06
JP6972022B2 (ja) 2021-11-24
MA44976A (fr) 2019-03-20
KR20190005193A (ko) 2019-01-15
CN115873904A (zh) 2023-03-31
AU2023200660B2 (en) 2024-12-12
US20230048658A1 (en) 2023-02-16
JP2022033734A (ja) 2022-03-02
US11384140B2 (en) 2022-07-12
IL262923B1 (en) 2023-04-01
CA3024027A1 (en) 2017-11-16
SG11201810040WA (en) 2018-12-28
AU2017263454A1 (en) 2018-12-06
WO2017197098A1 (en) 2017-11-16
TW201805422A (zh) 2018-02-16
IL262923B2 (en) 2023-08-01
BR112018073316A2 (pt) 2019-06-04
EA201892588A1 (ru) 2019-06-28
CL2018003210A1 (es) 2019-02-15
MX2018013882A (es) 2019-03-14
CN109219660B (zh) 2023-01-06
AU2023200660A1 (en) 2023-03-09
EP3455359B1 (en) 2022-03-09
US20190127452A1 (en) 2019-05-02
IL262923A (en) 2019-01-31
IL300591A (en) 2023-04-01
MX2022009674A (es) 2022-09-09
TWI821905B (zh) 2023-11-11
MX394682B (es) 2025-03-24
JP7344949B2 (ja) 2023-09-14
EP3455359A1 (en) 2019-03-20
TWI757291B (zh) 2022-03-11
AU2017263454B2 (en) 2023-02-09
ES2914118T3 (es) 2022-06-07
KR20210135635A (ko) 2021-11-15

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