BR112018073316A2 - seleção direta de células que expressam altos níveis de proteínas heteroméricas usando vetores de complementação intragênica da glutamina sintetase - Google Patents

seleção direta de células que expressam altos níveis de proteínas heteroméricas usando vetores de complementação intragênica da glutamina sintetase

Info

Publication number
BR112018073316A2
BR112018073316A2 BR112018073316A BR112018073316A BR112018073316A2 BR 112018073316 A2 BR112018073316 A2 BR 112018073316A2 BR 112018073316 A BR112018073316 A BR 112018073316A BR 112018073316 A BR112018073316 A BR 112018073316A BR 112018073316 A2 BR112018073316 A2 BR 112018073316A2
Authority
BR
Brazil
Prior art keywords
intragenic
cells expressing
high levels
glutamine synthetase
direct selection
Prior art date
Application number
BR112018073316A
Other languages
English (en)
Portuguese (pt)
Inventor
A Fomina Yadlin Dina
T Mcgrew Jeffrey
M Daris Kristine
Jagdish Agrawal Neeraj
R Ketchem Randal
P Munro Trent
Original Assignee
Amgen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amgen Inc filed Critical Amgen Inc
Publication of BR112018073316A2 publication Critical patent/BR112018073316A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1055—Protein x Protein interaction, e.g. two hybrid selection
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y104/00—Oxidoreductases acting on the CH-NH2 group of donors (1.4)
    • C12Y104/01—Oxidoreductases acting on the CH-NH2 group of donors (1.4) with NAD+ or NADP+ as acceptor (1.4.1)
    • C12Y104/01002—Glutamate dehydrogenase (1.4.1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y603/00—Ligases forming carbon-nitrogen bonds (6.3)
    • C12Y603/04—Other carbon-nitrogen ligases (6.3.4)
    • C12Y603/04004—Adenylosuccinate synthase (6.3.4.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y603/00—Ligases forming carbon-nitrogen bonds (6.3)
    • C12Y603/04—Other carbon-nitrogen ligases (6.3.4)
    • C12Y603/04005—Argininosuccinate synthase (6.3.4.5)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • C12N2015/8518—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic expressing industrially exogenous proteins, e.g. for pharmaceutical use, human insulin, blood factors, immunoglobulins, pseudoparticles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y603/00—Ligases forming carbon-nitrogen bonds (6.3)
    • C12Y603/01—Acid-ammonia (or amine)ligases (amide synthases)(6.3.1)
    • C12Y603/01002—Glutamate-ammonia ligase (6.3.1.2)

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
BR112018073316A 2016-05-11 2017-05-11 seleção direta de células que expressam altos níveis de proteínas heteroméricas usando vetores de complementação intragênica da glutamina sintetase BR112018073316A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662334966P 2016-05-11 2016-05-11
PCT/US2017/032139 WO2017197098A1 (en) 2016-05-11 2017-05-11 Direct selection of cells expressing high levels of heteromeric proteins using glutamine synthetase intragenic complementation vectors

Publications (1)

Publication Number Publication Date
BR112018073316A2 true BR112018073316A2 (pt) 2019-06-04

Family

ID=58873869

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112018073316A BR112018073316A2 (pt) 2016-05-11 2017-05-11 seleção direta de células que expressam altos níveis de proteínas heteroméricas usando vetores de complementação intragênica da glutamina sintetase

Country Status (17)

Country Link
US (2) US11384140B2 (enExample)
EP (2) EP3455359B1 (enExample)
JP (2) JP6972022B2 (enExample)
KR (2) KR102323519B1 (enExample)
CN (2) CN115873904A (enExample)
AU (2) AU2017263454B2 (enExample)
BR (1) BR112018073316A2 (enExample)
CA (1) CA3024027A1 (enExample)
CL (1) CL2018003210A1 (enExample)
EA (1) EA201892588A1 (enExample)
ES (1) ES2914118T3 (enExample)
IL (2) IL300591A (enExample)
MA (1) MA44976A (enExample)
MX (2) MX394682B (enExample)
SG (1) SG11201810040WA (enExample)
TW (2) TWI757291B (enExample)
WO (1) WO2017197098A1 (enExample)

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WO2018093331A1 (en) * 2016-11-16 2018-05-24 Agency For Science, Technology And Research Attenuated glutamine synthetase as a selection marker
BR112019024750A2 (pt) 2017-05-24 2020-06-09 Thoeris Gmbh uso de glutamina sintetase no tratamento da hiperamonemia
CN109988777A (zh) * 2017-12-29 2019-07-09 南京金斯瑞生物科技有限公司 谷氨酰胺合成酶基因以及应用
SG11202109642SA (en) * 2019-05-07 2021-10-28 Amgen Inc Vectors and expression systems for producing recombinant proteins
WO2023011442A1 (en) * 2021-08-03 2023-02-09 Shanghai Zhenge Biotechnology Co., Ltd. Selectable markers for eukaryotic expression system
CN118804978A (zh) * 2021-12-27 2024-10-18 上海臻格生物技术有限公司 用于重组生产系统的新型标志物
EP4496884A1 (en) * 2022-03-23 2025-01-29 Boehringer Ingelheim International Gmbh New glutamine synthetase variants as selection marker
AU2023238775A1 (en) 2022-03-23 2024-10-31 Boehringer Ingelheim International Gmbh Bacterial glutamine synthetase as selection marker in mammalian cells
WO2025037708A1 (en) * 2023-08-16 2025-02-20 Korea Advanced Institute Of Science And Technology Method for selection of cell line expressing high levels of recombinant protein using glutamine synthetase split expression vector
WO2025128343A1 (en) 2023-12-11 2025-06-19 Just-Evotec Biologics, Inc. Protein expression using trans-splicing and split selectable markers
CN118006685B (zh) * 2024-04-07 2024-07-23 上海碧博生物医药科技有限公司 一种快速的高表达单克隆细胞株构建方法
WO2025250814A1 (en) * 2024-05-31 2025-12-04 Amgen Inc. Intein based split glutamine synthetase engineering

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Also Published As

Publication number Publication date
CN109219660A (zh) 2019-01-15
TW202223093A (zh) 2022-06-16
KR102439719B1 (ko) 2022-09-02
EP4067493A1 (en) 2022-10-05
JP2019514417A (ja) 2019-06-06
JP6972022B2 (ja) 2021-11-24
MA44976A (fr) 2019-03-20
KR20190005193A (ko) 2019-01-15
CN115873904A (zh) 2023-03-31
AU2023200660B2 (en) 2024-12-12
US20230048658A1 (en) 2023-02-16
JP2022033734A (ja) 2022-03-02
US11384140B2 (en) 2022-07-12
IL262923B1 (en) 2023-04-01
CA3024027A1 (en) 2017-11-16
SG11201810040WA (en) 2018-12-28
AU2017263454A1 (en) 2018-12-06
WO2017197098A1 (en) 2017-11-16
TW201805422A (zh) 2018-02-16
IL262923B2 (en) 2023-08-01
EA201892588A1 (ru) 2019-06-28
CL2018003210A1 (es) 2019-02-15
MX2018013882A (es) 2019-03-14
CN109219660B (zh) 2023-01-06
AU2023200660A1 (en) 2023-03-09
EP3455359B1 (en) 2022-03-09
US20190127452A1 (en) 2019-05-02
IL262923A (en) 2019-01-31
IL300591A (en) 2023-04-01
MX2022009674A (es) 2022-09-09
KR102323519B1 (ko) 2021-11-09
TWI821905B (zh) 2023-11-11
MX394682B (es) 2025-03-24
JP7344949B2 (ja) 2023-09-14
EP3455359A1 (en) 2019-03-20
TWI757291B (zh) 2022-03-11
AU2017263454B2 (en) 2023-02-09
ES2914118T3 (es) 2022-06-07
KR20210135635A (ko) 2021-11-15

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