EA201700456A1 - Оптимизированная нуклеотидная последовательность и фармацевтическая композиция на ее основе с пролонгированной экспрессией трансгена vegf - Google Patents

Оптимизированная нуклеотидная последовательность и фармацевтическая композиция на ее основе с пролонгированной экспрессией трансгена vegf

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Publication number
EA201700456A1
EA201700456A1 EA201700456A EA201700456A EA201700456A1 EA 201700456 A1 EA201700456 A1 EA 201700456A1 EA 201700456 A EA201700456 A EA 201700456A EA 201700456 A EA201700456 A EA 201700456A EA 201700456 A1 EA201700456 A1 EA 201700456A1
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Eurasian Patent Office
Prior art keywords
pharmaceutical composition
nucleotide sequence
volf
transgene
basis
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EA201700456A
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English (en)
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EA039395B1 (ru
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Сергей Львович КИСЕЛЕВ
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Общество с ограниченной ответственностью "НекстГен"
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Publication of EA201700456A1 publication Critical patent/EA201700456A1/ru
Publication of EA039395B1 publication Critical patent/EA039395B1/ru

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A61K48/005—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy characterised by an aspect of the 'active' part of the composition delivered, i.e. the nucleic acid delivered
    • A61K48/0066—Manipulation of the nucleic acid to modify its expression pattern, e.g. enhance its duration of expression, achieved by the presence of particular introns in the delivered nucleic acid
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P21/00—Drugs for disorders of the muscular or neuromuscular system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/515—Angiogenesic factors; Angiogenin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/50—Vector systems having a special element relevant for transcription regulating RNA stability, not being an intron, e.g. poly A signal

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Epidemiology (AREA)
  • Physical Education & Sports Medicine (AREA)
  • Neurology (AREA)
  • Vascular Medicine (AREA)
  • Cardiology (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Выявлены точечные изменения нуклеотидной последовательности 3'UTR гена vegf, которые существенно увеличивают время жизни продукта его транскрипции - мРНК в клетках млекопитающих. Экспериментальным путем выбрана генная конструкция с оптимальным перечнем выявленных мутаций, характеризующаяся наибольшим временем жизни мРНК. Подтвержден биологический эффект разработанной генной конструкции и фармацевтической композиции на ее основе in vitro и in vivo.
EA201700456A 2015-05-26 2015-12-07 СПОСОБ ПОЛУЧЕНИЯ ГЕННОЙ КОНСТРУКЦИИ И ПЛАЗМИДНАЯ ДНК С УВЕЛИЧЕННЫМ ВРЕМЕНЕМ ЖИЗНИ мРНК ТРАНСГЕНА VEGF В КЛЕТКЕ МЛЕКОПИТАЮЩЕГО, ПЛАЗМИДНАЯ ДНК ГЕНА VEGF165, мРНК, ФАРМАЦЕВТИЧЕСКАЯ КОМПОЗИЦИЯ И ПРИМЕНЕНИЕ ПЛАЗМИДНОЙ ДНК EA039395B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
RU2015119768A RU2612497C2 (ru) 2015-05-26 2015-05-26 Оптимизированная нуклеотидная последовательность и фармацевтическая композиция на ее основе с пролонгированной экспрессией трансгена vegf
PCT/RU2015/000852 WO2016190774A1 (ru) 2015-05-26 2015-12-07 Оптимизированная нуклеотидная последовательность и фармацевтическая композиция на ее основе с пролонгированной экспрессией трансгена vegf

Publications (2)

Publication Number Publication Date
EA201700456A1 true EA201700456A1 (ru) 2018-06-29
EA039395B1 EA039395B1 (ru) 2022-01-21

Family

ID=57392933

Family Applications (1)

Application Number Title Priority Date Filing Date
EA201700456A EA039395B1 (ru) 2015-05-26 2015-12-07 СПОСОБ ПОЛУЧЕНИЯ ГЕННОЙ КОНСТРУКЦИИ И ПЛАЗМИДНАЯ ДНК С УВЕЛИЧЕННЫМ ВРЕМЕНЕМ ЖИЗНИ мРНК ТРАНСГЕНА VEGF В КЛЕТКЕ МЛЕКОПИТАЮЩЕГО, ПЛАЗМИДНАЯ ДНК ГЕНА VEGF165, мРНК, ФАРМАЦЕВТИЧЕСКАЯ КОМПОЗИЦИЯ И ПРИМЕНЕНИЕ ПЛАЗМИДНОЙ ДНК

Country Status (10)

Country Link
US (2) US9896493B2 (ru)
EP (1) EP3305901B1 (ru)
CN (1) CN107614687B (ru)
AU (1) AU2015396177B2 (ru)
BR (1) BR112017023058B1 (ru)
CA (1) CA2985608C (ru)
EA (1) EA039395B1 (ru)
MX (1) MX2017013206A (ru)
RU (1) RU2612497C2 (ru)
WO (1) WO2016190774A1 (ru)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114908089B (zh) * 2021-02-08 2024-03-15 上海细胞治疗集团有限公司 3’utr的构建方法和应用

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US6153407A (en) 1992-07-28 2000-11-28 Beth Israel Deaconess Medical Center Erythropoietin DNA having modified 5' and 3' sequences and its use to prepare EPO therapeutics
US5587300A (en) * 1994-04-26 1996-12-24 Wisconsin Ulumni Research Foundation Method to increase regulatory molecule production
US6613563B1 (en) 1997-03-14 2003-09-02 Selective Gentics, Inc. Viral vectors with modified tropism
US6627436B2 (en) 1997-10-31 2003-09-30 Stratagene Vector for gene expression in prokaryotic and eukaryotic systems
CA2322232A1 (en) 1998-03-13 1999-09-16 American Home Products Corporation Lyophilized polynucleotide composition, method of preparation, and uses thereof
US7598079B2 (en) * 1998-12-24 2009-10-06 Novation Pharmaceuticals, Inc. Assay for identifying compounds which affect stability of mRNA
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CA2451311A1 (en) * 2001-06-20 2003-01-03 Ludwig Institute For Cancer Research Stimulation of vascularization with vegf-b
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CN100431611C (zh) 2002-07-04 2008-11-12 朱静亚 多肽－脂质体与人血管内皮生长因子基因重组质粒复合物及用途
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CA2753362C (en) 2009-02-24 2018-06-19 The Scripps Research Institute Reengineering mrna primary structure for enhanced protein production
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RU2431669C2 (ru) * 2009-06-23 2011-10-20 Государственное образовательное учреждение высшего профессионального образования "Казанский государственный университет им. В.И. Ульянова-Ленина" Способ получения лекарственного препарата генетически модифицированных клеток
RU2542385C2 (ru) * 2012-08-31 2015-02-20 Общество с ограниченной ответственностью "НекстГен" Способ получения фармацевтической композиции для индукции развития кровеносных сосудов в тканях, фармацевтическая композиция, полученная этим способом, и способ лечения ишемии тканей и/или органов человека

Also Published As

Publication number Publication date
US20160347803A1 (en) 2016-12-01
US9896493B2 (en) 2018-02-20
EP3305901A4 (en) 2018-10-24
US20180086800A1 (en) 2018-03-29
RU2015119768A (ru) 2016-12-20
RU2612497C2 (ru) 2017-03-09
BR112017023058A2 (pt) 2018-10-16
AU2015396177B2 (en) 2019-02-28
WO2016190774A1 (ru) 2016-12-01
MX2017013206A (es) 2018-02-15
EP3305901B1 (en) 2020-09-30
EP3305901A1 (en) 2018-04-11
CN107614687B (zh) 2021-05-28
US10040837B2 (en) 2018-08-07
CA2985608A1 (en) 2016-12-01
CN107614687A (zh) 2018-01-19
BR112017023058B1 (pt) 2024-03-05
AU2015396177A1 (en) 2017-11-02
CA2985608C (en) 2021-06-15
EA039395B1 (ru) 2022-01-21

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