KR102420704B1 - 염 처리를 통해 실리카 나노입자에 고정화된 목적 단백질의 안정성을 증가시키는 방법 - Google Patents

염 처리를 통해 실리카 나노입자에 고정화된 목적 단백질의 안정성을 증가시키는 방법 Download PDF

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KR102420704B1
KR102420704B1 KR1020200072553A KR20200072553A KR102420704B1 KR 102420704 B1 KR102420704 B1 KR 102420704B1 KR 1020200072553 A KR1020200072553 A KR 1020200072553A KR 20200072553 A KR20200072553 A KR 20200072553A KR 102420704 B1 KR102420704 B1 KR 102420704B1
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South Korea
Prior art keywords
silica
protein
bca
immobilized
target protein
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KR1020200072553A
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English (en)
Korean (ko)
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KR20210155257A (ko
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조병훈
임균택
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경상국립대학교산학협력단
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Priority to KR1020200072553A priority Critical patent/KR102420704B1/ko
Priority to PCT/KR2021/004311 priority patent/WO2021256674A1/fr
Publication of KR20210155257A publication Critical patent/KR20210155257A/ko
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Publication of KR102420704B1 publication Critical patent/KR102420704B1/ko

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K17/00—Carrier-bound or immobilised peptides; Preparation thereof
    • C07K17/14—Peptides being immobilised on, or in, an inorganic carrier
    • C—CHEMISTRY; METALLURGY
    • C01—INORGANIC CHEMISTRY
    • C01B—NON-METALLIC ELEMENTS; COMPOUNDS THEREOF; METALLOIDS OR COMPOUNDS THEREOF NOT COVERED BY SUBCLASS C01C
    • C01B33/00—Silicon; Compounds thereof
    • C01B33/113—Silicon oxides; Hydrates thereof
    • C01B33/12—Silica; Hydrates thereof, e.g. lepidoic silicic acid
    • C01B33/18—Preparation of finely divided silica neither in sol nor in gel form; After-treatment thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/01—Hydro-lyases (4.2.1)
    • C12Y402/01001—Carbonate dehydratase (4.2.1.1), i.e. carbonic anhydrase
    • C—CHEMISTRY; METALLURGY
    • C01—INORGANIC CHEMISTRY
    • C01P—INDEXING SCHEME RELATING TO STRUCTURAL AND PHYSICAL ASPECTS OF SOLID INORGANIC COMPOUNDS
    • C01P2004/00—Particle morphology
    • C01P2004/60—Particles characterised by their size
    • C01P2004/64—Nanometer sized, i.e. from 1-100 nanometer
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/35—Fusion polypeptide containing a fusion for enhanced stability/folding during expression, e.g. fusions with chaperones or thioredoxin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Inorganic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
KR1020200072553A 2020-06-15 2020-06-15 염 처리를 통해 실리카 나노입자에 고정화된 목적 단백질의 안정성을 증가시키는 방법 KR102420704B1 (ko)

Priority Applications (2)

Application Number Priority Date Filing Date Title
KR1020200072553A KR102420704B1 (ko) 2020-06-15 2020-06-15 염 처리를 통해 실리카 나노입자에 고정화된 목적 단백질의 안정성을 증가시키는 방법
PCT/KR2021/004311 WO2021256674A1 (fr) 2020-06-15 2021-04-07 Procédé permettant d'augmenter la stabilité d'une protéine cible immobilisée dans une nanoparticule de silice par traitement salin

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1020200072553A KR102420704B1 (ko) 2020-06-15 2020-06-15 염 처리를 통해 실리카 나노입자에 고정화된 목적 단백질의 안정성을 증가시키는 방법

Publications (2)

Publication Number Publication Date
KR20210155257A KR20210155257A (ko) 2021-12-22
KR102420704B1 true KR102420704B1 (ko) 2022-07-14

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KR1020200072553A KR102420704B1 (ko) 2020-06-15 2020-06-15 염 처리를 통해 실리카 나노입자에 고정화된 목적 단백질의 안정성을 증가시키는 방법

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KR (1) KR102420704B1 (fr)
WO (1) WO2021256674A1 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114702832B (zh) * 2022-06-07 2022-08-23 江西中医药大学 玉米蛋白-二氧化硅复合物乳剂及其制备方法和应用

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2762488B1 (fr) * 2011-09-30 2018-11-21 Korea University Research and Business Foundation, Sejong Campus Peptide pour la synthèse de la silice, et son utilisation
KR101990773B1 (ko) * 2012-08-28 2019-06-20 고려대학교 세종산학협력단 실리카를 합성할 수 있는 변형 녹색형광단백질 및 이의 용도
KR102107515B1 (ko) * 2016-04-22 2020-05-07 고려대학교 세종산학협력단 실리카 합성을 위한 재조합 단백질 및 이의 용도
KR101964155B1 (ko) * 2016-05-10 2019-04-02 포항공과대학교 산학협력단 바이러스 피막 단백질 및 실리카 형성 펩타이드를 포함하는, 융합 단백질 및 이의 용도
WO2018152270A1 (fr) * 2017-02-15 2018-08-23 Massachusetts Institute Of Technology Molécules synthétiques à base de peptides et nanostructures de silice
KR102704701B1 (ko) * 2018-11-08 2024-09-10 한국전력공사 탄산무수화효소 및 실리카 친화성 펩타이드를 포함하는 융합 단백질 및 이의 제조방법
KR102401223B1 (ko) * 2019-02-15 2022-05-25 고려대학교 세종산학협력단 실리카 형성 펩타이드를 포함하는 링커 조성물

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Jun-Kun Lai et al., ‘Efficient and stable enzyme immobilization in a block copolypeptide vesicle-templated biomimetic silica support’, Colloids and Surfaces B: Biointerfaces, Vol. 80, pp. 51-58, 2010.*

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KR20210155257A (ko) 2021-12-22
WO2021256674A1 (fr) 2021-12-23

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