KR102419683B1 - Asthma relief or therapeutic biologic composition - Google Patents

Abstract

The present invention discloses a natural complex for relieving asthma.
According to the natural complex for relieving asthma according to the present invention, it is characterized in that it contains chrysanthemum, licorice, astragalus, angelfish, and native honey. , Unlike direct injection, it is easy to take orally to relieve asthma and to relieve asthma that can be treated innovatively.

Description

Asthma relief or therapeutic biologic composition

The present invention relates to a natural complex for relieving asthma, which can be used as a medicinal acupuncture component of traditional herbal acupuncture by applying it to traditional oriental acupuncture, or can be easily taken orally to relieve asthma and treat epoch-making asthma, unlike direct injection. It relates to a natural complex that relieves the

Asthma is a chronic allergic respiratory disease, which refers to a condition in which the bronchi in the lungs are very sensitive. , is an allergic disease caused by an allergic inflammatory reaction of these bronchial tubes.

These symptoms appear repeatedly, causing the bronchial mucosa to swell due to inflammation of the bronchial tubes, and the bronchial muscles spasm and block the bronchial tubes.

These allergic diseases are caused by the interaction of genetic factors and environmental factors, and in most cases, are a representative intractable chronic disease that is not easy to cure. It is called an allergen, and typical allergens include house dust mites, pollen, animal hair or dander, cockroaches, food, and drugs. These include physical activity, climate change, yellow dust, and stress.

In addition, asthma is a chronic inflammatory disease of the airways, and airway inflammation and airway hypersensitivity cause symptoms such as cough, wheezing, shortness of breath, and chest tightness. Mucosal mast cells secrete airway constriction mediators such as histamine (bronchial constriction and inflammatory response), leukptrienes, and prostaglandin D2 (bronchoconstrictor), and activate eosinophils and TCR invariant natural killer T cells. Symptoms are caused by secretion of inflammatory substances such as cytokines (regulating the overall inflammatory response) and chemokines (mobilizing inflammatory cells) that damage airway epithelial cells.

In addition, along with the occurrence of hypertrophy and hyperplasia of smooth muscle cells, the blood vessels in the airway proliferate under the influence of growth factors such as vascular endothelial growth factor (VEGF), and the thickness of the airway wall increases.

On the other hand, asthma is a chronic and recurrent disease, and treatment methods include drug therapy using drugs to relieve symptoms and prevent recurrence, and to find substances that cause asthma to minimize exposure to causative allergens and avoid exacerbating factors. There is avoidance therapy, and there are treatment methods such as immunotherapy, which improves allergic constitution by injecting a small amount of a causative agent.

Among them, asthma treatment drugs used in drug therapy are divided into symptom relievers that relieve narrowed bronchi within a short time and disease control agents that prevent asthma attacks by suppressing allergic inflammation of the bronchi. As representative symptom relievers, beta-2 agonists (agonists), xanthine drugs such as theophylline, and anticholinergics (anticholinergics) are widely used. However, symptom relievers such as beta-2 agonists do not fundamentally treat asthma, so they must be used regularly and may cause side effects in the body.

If steroids are also used for a long time, serious side effects such as blood sugar rise, blood pressure rise, weight gain, mood changes, osteoporosis, gastric ulcer, etc. There is a problem in that it does not respond at all to a very dangerous state.

Therefore, the technical problem to be solved by the present invention is to solve asthma, which can be used as a medicinal acupuncture component of acupuncture or directly by injection by applying it to traditional oriental acupuncture, or by taking it orally, which can be easily treated orally to relieve asthma and achieve breakthrough treatment to provide a natural complex that

The present invention provides a natural complex for relieving asthma, characterized in that it contains rice seed, licorice, Astragalus, Angelica, native honey in order to solve the above-described technical problem.

According to another embodiment of the present invention, the seedling may include its roots.

According to another embodiment of the present invention, it may be to use 8 to 12 parts by weight of licorice, 8 to 12 parts by weight of astragalus, 10 to 16 parts by weight of angelicae, 100 to 200 parts by weight of native honey with respect to 100 parts by weight of the rice seedlings.

According to another embodiment of the present invention, it may be a mixture of the extracts of the rice seedlings, licorice, astragalus, and Korean angelfish and native honey.

According to another embodiment of the present invention, the native honey may further include royal jelly, pollen, and beeswax in addition to honey.

According to another embodiment of the present invention, the mixing of the extract and native honey may be performed so that the viscosity of the mixture reaches 4000 to 8000 cP.

According to another embodiment of the present invention, the rice seedlings, licorice, Astragalus, Angelica, and native honey may be prepared in the form of a ring by drying and then grinding and mixing with native honey.

According to another embodiment of the present invention, it may be prepared in the form of a jelly in a state in which the extract of the active ingredient is extracted from the rice seedlings, licorice, Astragalus, and Angelica, and mixed with native honey.

According to the natural complex for relieving asthma according to the present invention, it is possible to relieve asthma and treat epoch-making asthma by easily taking it orally, unlike conventionally applied to oriental acupuncture and used as a medicinal acupuncture component of acupuncture, or directly injected by injection. has the effect of resolving

Hereinafter, the present invention will be described in detail.

While this specification contains numerous specific implementation details, they should not be construed as limitations on the scope of any invention or claim, but rather as descriptions of features that may be specific to particular embodiments of particular inventions. should be understood Certain features that are described herein in the context of separate embodiments may be implemented in combination in a single embodiment.

On the other hand, the embodiments of the present invention disclosed in the present specification and drawings are merely presented as specific examples to aid understanding, and are not intended to limit the scope of the present invention. It will be apparent to those of ordinary skill in the art to which the present invention pertains that other modifications based on the technical spirit of the present invention can be implemented in addition to the embodiments disclosed herein.

The natural complex for relieving asthma according to the present invention is characterized in that it contains rice seedlings, licorice, astragalus, Angelica, and native honey.

As a perennial herbaceous plant belonging to the chrysanthemum family, it was called Jangpi (獐矣皮) and Jeouihwacha (猪矣和次) as the name of Iduhyang in the Goryeo Dynasty, and Gyenoji (季奴只) in the Joseon Dynasty. , 『Donguibogam』, 『Hunmongjahoe (訓蒙字会)』, 『Chongagugeukbang』, etc. are recorded as 'Gyerogi', and the scientific name is Adenophora remotiflora (S. et Z). .) It is MIQ., but it is also called Togilgyeong because the root resembles a bellflower.

This plant grows in a slightly shady place in the forest. It is 40-100 cm high, has thick roots, and has alternate heart-shaped leaves, 5-20 cm long and 3-8 cm wide, with sharp tips and sharp edges. It has sawtooth, and the flowers are purple and bloom from August to September.

In particular, the root of Togilgyeong has antipyretic, detoxifying, and expectorant properties, so it is used when coughing and fever with a cold, and it is also used to clear phlegm. It promotes diuresis in pregnant women after childbirth and has the effect of relieving drug addiction.

The licorice (Glycyrrhiza uralensis FISCH) is used as a herbal medicine by drying the root, and it is called licorice because its taste is sweet. In particular, glycyrrhizin detoxifies diphtheria toxin, tetanus toxin, codeine hydrochloride, strychnine acetate, snake venom, pufferfish venom, etc. It has a depressant effect, and promotes the secretion of bile, and also acts as an antitussive and analgesic.

In addition, Astragalus propinquus or Mongolian milkvetch (黃耆, Astragalus propinquus, or Mongolian milkvetch) is a perennial plant distributed in Korea, China, and Japan that grows in crevices of rocks in mountainous areas, is 40-70 cm high, has white soft fine hairs, and has a stalk. The leaf is a radix one-time right-sided bifoliate composed of 6-11 pairs of leaflets. The leaflet is about 1-2cm long and ovate oval, the leaf edge is flat, the stipule is lanceolate with a long pointed tip, and the cost is from the leaf axil to the raceme. Long spikelets come out, 5-10 flowers hang, yellow-white flowers bloom in July-August, about 2cm long, small peduncle about 3mm long, calyx about 5mm long, blackish brown hairs, split into 5, stamens There are 10, and the pods are long oval, pointed at both ends, 2-3cm long, and contain 5-7 seeds.

It is commonly cultivated as a medicinal herb, and in oriental medicine, collected in autumn, removing the outcrops and fine roots, and dried in sunlight is called astragalus of herbal medicine. It is also prescribed for qi-hyeol collapse, uterine dislocation, visceral drainage, cold sweat, peripheral nerve palsy, hemiplegia, edema, and chronic nephritis.

On the other hand, the angelica gigas root grows wild in deep mountains, but is mainly cultivated, the stem is about 1 to 2 m in height, and the root is used as a drug. is about 20 to 25 cm, and the diameter is about 1 to 5 cm, and the main ingredients are essential oil, uterine excitatory ingredients, sucrose, vitamin E, etc. , is known to have a strong property of entering and acting mainly in the heart, liver, and spleen.

In addition, it is effective for menstrual cramps, and it is used in combination with Earl Potion, Yeonhosak, and Hyangbuja. In addition, it is also effective for amenorrhea and menstrual irregularities. When the body looks dry and exhausted due to non-hyeol-heo, it is sometimes used as an angelic yanghyeol-geonbi.

In addition, Angelica is used for yang and yangyang when dizzy, closed eyes, ringing in the ears, and muscle cramps, and is also used for eohyeol. It is known to improve blood circulation and relieve pain.

On the other hand, the native nectar can be collected by using a soil-bong, and the soil-bong does not move and grows bees only in one place. This is because it flies freely in the fields and mountains, collecting nectar from various flowers.

Another characteristic of native honey is that it is harvested only once a year, after frost in late autumn. As native honey matures for a long time in the beehive, almost no moisture is lost, so the honey is thick, and royal jelly and pollen are added to the honey. It is different from beekeeping honey in that it contains beeswax.

This nectar is stored by enzymatic action by storing a small amount of nectar contained in flowers in the honey glands of bees, and the royal jelly is a substance secreted by bees and is the main amount of the queen bee larvae, and pollen is used for the legs and body of the feet in the stamens of flowers. It is buried and stored through the enzymatic action of bees with honey, and contains essential amino acids, high-unit natural vitamins, fats, carbohydrates, and minerals. will be used

On the other hand, the above-mentioned mother seed, licorice, Astragalus, Angelica, and native honey content is important, based on 100 parts by weight of the rice seedling, licorice in 8 to 12 parts by weight, Astragalus 8 to 12 parts by weight, Angelica 10 to 16 parts by weight, native honey 100 to 200 parts by weight may be used.

The above-mentioned rice seed, licorice, Astragalus, Angelica, and native honey can be taken in the form of pills by drying and then grinding and mixing with native honey.

In addition, the extract of the active ingredient from the seedlings, licorice, astragalus, and angelfish can be made and taken in the form of a jelly mixed with native honey.

First, the herbal medicines in a dried or dried state, licorice, astragalus, and Angelica keiskei are immersed in an aqueous ethanol solution to extract active ingredients from the herbal medicines in a hot water bath.

Here, even when extracting the essence with a bath, there is a risk of fire due to ethanol, so the concentration of ethanol is preferably 5 to 15% by weight. Conversely, if it is less than 5% by weight, it may be difficult to extract the active ingredient, so there may be inconvenience of frequently replenishing the aqueous ethanol solution.

Next, after starting the bath, 10 to 60% of the ethanol aqueous solution remains with respect to the initial input amount, and the bath is terminated to obtain the extract extracted from the herbal medicines.

If the ethanol aqueous solution is less than 10% of the initial input amount after the start of the bath, the extract may stick to the fiber components of the herbal medicine. Conversely, if it exceeds 60%, it may be difficult to sufficiently extract the extract.

Of course, it is possible to additionally extract the extract by pressing the herbal medicines after obtaining the extract.

Next, it is uniformly mixed with native honey. To prevent sediment from occurring in the essence, the native honey is immersed in the essence and shaken or rotated to mix. It is important to maintain the temperature between 80 °C and 100 °C.

If the temperature of the extract is less than 80 ℃ during mixing, it is difficult to evaporate the aqueous ethanol solution from the extract, so an excess of alcohol may remain. Mixing can be difficult.

On the other hand, when the viscosity of the mixture of the extract and native honey reaches 4000 to 8000 cP, the stirring is terminated and natural cooling is performed. It may be difficult to achieve uniform mixing.

Examples and Comparative Examples

In Example 1, 1600 g of rice seedlings, 150 g of licorice, 150 g of astragalus, and 200 g of Angelica were dried and finely ground and mixed with 2.2 kg of native honey to prepare a ring with a diameter of 0.5 to 2 mm. g, 150 g of licorice, 150 g of Astragalus, 200 g of Angelica basil, pulverized and immersed in an aqueous ethanol solution (10% by weight of ethanol) and maintained at 80 ° C. In the remaining state, the bath was terminated to obtain the essence through filtration, and the mixture was stirred with local honey while maintaining 90 ° C. When the viscosity of the honey and mixture reached 6000 cP, stirring was terminated, and a jelly-like natural complex was prepared.

As a comparative example, 100 parts by weight of dried turmeric, 100 parts by weight of hyangbuja, 100 parts by weight of peony, 100 parts by weight of ginger, 100 parts by weight of angelicae, 75 parts by weight of safflower, 75 parts by weight of licorice, distilled water and an aqueous solution of ethanol with a concentration of 62.5% by weight 1: Prepare a mixed solvent mixed in a 0.25 volume ratio, and then, 3.25L of the mixed solvent per 1 kg of the herbal compound was mixed to prepare a mixed solution, and then, the mixed solution was stirred and heated to 80 ~ 82 ℃ and distilled to obtain a concentrated extract, which was mixed with sterile physiological saline (N/S) (Lot No: 12062, source: JW Pharmaceutical) and diluted to a concentration of 35% by volume to prepare an injection.

Experimental Example 1

Asthma induced and administered with ovalbumin in BALB/c mice. Total white blood cell count (WBC), eosinophil and differential cell count in bronchoalveolar lavage fluid (BALF). , IgE) was measured.

The rats used in this experiment are widely used for asthma tests. They are females at 6 weeks of age at the time of acquisition and 7 weeks of age at the time of this experiment. Red oil magic was used during the acclimatization period and black oil magic was used during administration and observation period, and an individual identification card was attached to the breeding box. %, ventilation frequency 10 - 20 times/hr, lighting time 12 hours, and illuminance of 150 ~ 300 Lux were bred in a breeding area. The animals were bred in boxes, and the weights of animals determined to be healthy during the acclimatization period were measured and ranked, and the average weight of each group was uniformly distributed.

As an asthma trigger, refrigerated ovalbumin (OVA) (Catalog number: A5503, source: Sigma Aldrich, Louis, MO, USA) was used, and refrigerated aluminum hydroxide (Alum) was used as an immune adjuvant. ) (Catalog number: 77161, source: Thermo Fisher Scientific lnc, Waltham, MA, USA,) was used.

And, as a positive control material, dexamethasone (DEX) stored at room temperature (Product Number: 13099, Source: SAMNAM Pharm, Chungnam, Korea) was used.

The intraperitoneal injection for inducing asthma in mice was injected twice a week with OVA 20 μg+, Alum 1 mg+, and sterile physiological saline 500 μL/head for 2 weeks.

In addition, the asthma-induced mice were injected with physiological saline for the control group and dexamethasone for the positive group, and were injected twice a week for 4 weeks as shown in Table 1 below in Comparative Example, and the injection amount and oral dosage were indicated.

In addition, after 4 weeks, white blood cells (WBC), eosinophils, and IgE were measured from mouse blood, and the average measurement results are shown in Table 2 below.

division Asthma Expressing Mice Injection volume (μl/g), oral dose (mg) physiological saline DEX herbal composition control 3 (Female) 30 positive group 3 (Female) 28 0 comparative example 3 (Female) 30 Example 1 3 (Female) 30 Example 2 3 (Female) 30

division WBC (Х10 ³ cells/μl) Eosinic water (Х10 ² cells/μl) IgE (μg/ml) control 61.20 2.32 59.53 positive group 39.83 1.41 23.45 comparative example 45.50 1.82 29.77 Example 1 40 1.7 25 Example 2 38 1.6 24

Referring to Table 2, in the case of the positive group and the comparative example compared to the control group, it can be seen that WBC, eosinophil and IgE are significantly lowered, and in the case of Examples 1 and 2, WBC, eosinophil and IgE measurement values are higher than that of the positive group Although slightly higher, compared with the control group, WBC, eosinophils and IgE were significantly lowered.

In contrast, in the case of the comparative example, although the WBC, eosinophil and IgE measurement values were lower than that of the control group, the WBC, eosinophil and IgE reducing effect was decreased compared to the positive group or Examples.

Experimental Example 2

For 20 asthma patients, 10 each were given the herbal composition according to Examples 1 and 2, and the results are shown in Tables 3 and 4 below, respectively. check for asthma relief)

division 4 weeks Week 8 Week 12 very relaxed 5 6 9 ease 2 2 One slightly relieved 2 One 0 no change One One 0

division 4 weeks Week 8 Week 12 very relaxed 6 8 9 ease 2 2 One slightly relieved 2 0 0 no change 0 0 0

Through the above experiment, it was confirmed that asthma can be alleviated by the herbal composition according to the present invention.

Claims (8)

Contains rice seedlings, licorice, Astragalus, Angelica, and local honey,
After immersing the dried or pulverized herbal medicines in an aqueous solution of ethanol with an ethanol concentration of 5 to 15% by weight, active ingredients are extracted from the herbal medicines in a bath, and after starting the bath After completing the bath with 10 to 60% of the initial amount of ethanol solution remaining, the extract is obtained from herbal medicines, and the temperature of the extract is maintained at 80 ° C to 100 ° C during mixing and uniformly mixed with local honey. A natural complex for relieving asthma, characterized in that it is prepared so that the viscosity of the mixture is 4000 to 8000 cP.
The method of claim 1,
The mother seed is a natural complex for relieving asthma, characterized in that it contains the root.
The method of claim 1,
8 to 12 parts by weight of licorice, 8 to 12 parts by weight of astragalus, 10 to 16 parts by weight of angelicae, and 100 to 200 parts by weight of native honey based on 100 parts by weight of the rice seedlings, a natural complex for relieving asthma.
delete The method of claim 1,
The native honey is a natural complex to relieve asthma, characterized in that it further comprises royal jelly, pollen, and beeswax in addition to honey.
delete The method of claim 1,
The mother seed, licorice, Astragalus, Angelica, and native honey are dried and then crushed and mixed with native honey to relieve asthma, characterized in that they are prepared in the form of a pill.
The method of claim 1,
A natural complex for relieving asthma, characterized in that it is prepared in the form of a jelly mixed with native honey by extracting the extract of the active ingredient from the rice seedlings, licorice, astragalus, and angelfish.