KR20130036800A - A composition for stimulating immune response comprising an extract of angelicae gigantis radix and astragali radix - Google Patents

Abstract

PURPOSE: A composition for increasing immunity is provided to increase an immune function by changing factors related to fatigue. CONSTITUTION: A pharmaceutical composition for increasing immunity comprises Angelica and Astragalus membranceus bunge extract as active ingredient. The extract of pharmaceutical composition for increasing immunity is water extracts. The mixing ratio of Angelica and Astragalus membranceus bunge extract is 2:5. Angelica and Astragalus membrabceus extract are included as an active ingredient.

Description

A composition for enhancing immunity comprising Angelica extract and Angelica extract as active ingredient {A composition for Stimulating Immune Response Comprising an extract of Angelicae gigantis Radix and Astragali Radix}

The present invention relates to a composition for enhancing immunity comprising the extract of Angelica sinensis and Astragalus as an active ingredient.

Immunity is largely divided into innate immunity that has been born since birth and acquired immunity that is acquired by adapting to life. Here, innate immunity, also known as 'natural immunity', is a nonspecific response to the antigen and does not show a special memory action. Congenital immune systems include skin, mucous tissues that block antigen invasion, acidic acid, and phagocytic cells such as macrophage (macrophage) and polymorphonuclear leukocytes that are responsible for phagocytosis to remove invaders. This innate immunity actually protects against most infections. On the other hand, acquired immunity has a memory function for the first invasive antigen, and when invasion occurs again, it is characterized by specific reaction to effectively remove the antigen.

When this immune function is reduced, various immune diseases such as asthma, seasonal or perennial rhinitis, allergic sinusitis, conjunctivitis, atopic dermatitis, urticaria, hemolysis of red blood cells, acute glomerulonephritis may occur. Prevention of these immune diseases is more important than cure, but currently available therapeutics are difficult to take for prevention. Thus, there is a need for a method for preventing and treating immune dysfunction without taking an adjuvant with possible side effects.

Therefore, many studies have been conducted on new drug research and drug development that can prevent and treat immune dysfunction, and in particular, attention has been focused on the development of anticancer substances in natural products with few side effects.

On the other hand, the concept of an adjuvant to enhance immune function has been proposed by Roman, and it is known that the administration of any immunopotentiator with the vaccine further enhances the effect of the vaccine as compared with the administration of the vaccine alone. Started. In addition, the immunopotentiator refers to all substances that raise the specific or non-specific, cellular and humoral immune response of the host, has recently attracted a lot of attention as the use of immune fortification in the treatment of infections or tumors.

 Angelicae Gigantis Radix, on the other hand, is a dried perennial herb, the perennial herb, which is sweet and tastes very warm. Astragali Radix is a perennial plant of the dicotyledonous rosewood legume, and is distributed in Korea, Japan, Manchuria, northeastern China and eastern Siberia. It is often cultivated as a herb, and it is known that herbal medicine is harvested in the fall to remove outcrops and fine roots, and dried in the sun.

Accordingly, the present inventors have long-term use because there are no side effects in vivo, and as a result of continuing research on the development of materials from natural resources to find new immunopotentiating compositions that can prevent and treat immune dysfunction, the extracts of Angelica and Astragalus are fatigued. The present invention was completed by confirming that the factors related to) increase immune function and activate IL-12, TNF-α, NO, and NF-kB, and thus can be usefully used as an adjuvant. .

It is an object of the present invention to provide a pharmaceutical composition for enhancing immunity comprising the extract of Angelica gigas and Astragalus as an active ingredient.

An object of the present invention to provide a pharmaceutical composition for the prevention or treatment of immune diseases, including the extract of Angelica gigas and Astragalus as an active ingredient.

Still another object of the present invention is to provide an immune enhancing health functional food comprising the extract of Angelica sinensis and Astragalus as an active ingredient.

In order to achieve the above object, the present invention provides a pharmaceutical composition for enhancing immunity comprising Angelica extract and Angelica extract as an active ingredient.

In another aspect, the present invention provides a pharmaceutical composition for preventing or treating immunological diseases, including extracts of Angelica gigas and Astragalus as active ingredients.

In another aspect, the present invention provides a health functional food for immune enhancement comprising Angelica extract and Angelica extract as an active ingredient.

Immunity-enhancing compositions comprising extracts of Angelica gigas and Astragalus according to the present invention as active ingredients increase fatigue function by changing factors related to fatigue and activate IL-12, TNF-α, NO and NF-kB. It has a function to make it useful as an immune enhancer and an agent for preventing or treating immune diseases.

1 and 2 are pictures confirming the effect of the extract of Angelica and Astragalus (DGBHT) in FST.
Figure 3 is a figure confirming the effect of the extract of Angelica and Astragalus (DGBHT) on Glc and TP in mouse blood.
Figure 4 is a figure confirming the effect of the extract of Angelica and Astragalus (DGBHT) on LDH, CK and BUN in mouse blood.
Figure 5 is a figure confirming the effect of the extract of Angelica and Astragalus (DGBHT) on the production of IL-12 in mouse peritoneal macrophages.
Figure 6 is a figure confirming the effect of the extract of Angelica and Astragalus (DGBHT) on the production of TNF-α in mouse peritoneal macrophages.
Figure 7 is a figure confirming the effect of the extract of Angelica and Astragalus (DGBHT) on the production of NO in mouse peritoneal macrophages.
8 is a figure confirming the effect of the extract of Angelica and Astragalus (DGBHT) on the expression of iNOS in mouse peritoneal macrophages.
9 is a diagram confirming the effect of the extract of Angelica and Astragalus (DGBHT) on the expression of NK-kB in mouse peritoneal macrophages.

Hereinafter, the present invention will be described in more detail.

In one embodiment, the present invention provides a pharmaceutical composition for enhancing immune system comprising Angelica extract and Angelica extract as an active ingredient.

Angelica and Astragalus extract of the present invention can be obtained as follows.

Angelica and Astragalus are washed with water to remove foreign substances, dried in the shade and crushed. Angelica and Astragalus can be used without limitation, such as cultivated or commercially available. Appropriate amount of solvent is added to the ground Angelica and Astragalus to ensure complete immersion. As the extraction solvent, a solvent selected from water, a lower alcohol having 1 to 4 carbon atoms or a mixed solvent thereof is preferable, and more preferably water. Next, the extract of Angelica and Astragalus obtained above was filtered and concentrated under reduced pressure to obtain a final extract. The mixing ratio of Angelica and Astragalus may be 1: 1 to 1:10, preferably 2: 5.

In still another aspect, the present invention provides a pharmaceutical composition for preventing or treating immune diseases, including the extract of Angelica gigas and Astragalus as an active ingredient.

In the present invention, "immune disease" includes various inflammatory diseases, allergic diseases and cancers caused by abnormal immune system of the living body, for example, cold, asthma, pneumonia, allergic rhinitis, allergic conjunctivitis, atopic dermatitis Dermatitis, allergy, rheumatoid arthritis, Alzheimer's disease, autoimmune thyroid disease, inflammatory bowel disease, aplastic anemia, lupus erythematosus and psoriasis, and the like, but are not limited thereto.

The composition of the present invention may include one or more known active ingredients having an effect of preventing or treating immunity enhancer or immune disease together with Angelica sinensis and Astragalus extract.

The composition of the present invention can be used alone or in combination with methods using surgery, radiation therapy, hormone therapy, chemotherapy and biological response modifiers for the prevention and treatment of immune enhancing or immune diseases.

The pharmaceutical compositions of the present invention may further comprise suitable carriers, excipients and diluents conventionally used in the manufacture of pharmaceutical compositions. The pharmaceutical composition according to the present invention may be formulated in the form of oral, granule, tablet, capsule, suspension, emulsion, syrup, aerosol or the like oral preparation, external preparation, suppository and sterilized injection solution according to a conventional method . Suitable formulations known in the art are preferably those disclosed in Remington ' s Pharmaceutical Sciences, Mack Publishing Company, Easton PA. Examples of carriers, excipients and diluents that can be included in the pharmaceutical composition of the present invention include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium Silicates, cellulose, methylcellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. In the case of formulation, a diluent or excipient such as a filler, an extender, a binder, a wetting agent, a disintegrant, or a surfactant is usually used. Solid preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, and the solid preparations may include at least one excipient such as starch, calcium carbonate, sucrose in the extract. ) Or lactose, gelatin and the like are mixed. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Oral liquid preparations include suspensions, solvents, emulsions, and syrups, and may include various excipients, such as wetting agents, sweeteners, fragrances, and preservatives, in addition to commonly used simple diluents such as water and liquid paraffin. . Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Examples of the suspending agent include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like. As the base of the suppository, witepsol, macrogol, tween 61, cacao butter, laurin butter, glycerogelatin and the like can be used.

The preferred dosage of the pharmaceutical composition of the present invention varies depending on the condition and the weight of the patient, the degree of disease, the type of drug, the route of administration and the period of time, but can be appropriately selected by those skilled in the art. However, for the desired effect, the composition of the present invention may be administered at 0.0001 to 10000 mg / kg, preferably at 0.001 to 1000 mg / kg. The administration may be carried out once a day or divided into several times. The dose is not intended to limit the scope of the invention in any way.

The pharmaceutical composition of the present invention can be administered to mammals such as rats, mice, livestock, humans, and the like in various routes. All modes of administration may be expected, for example, by oral, rectal or intravenous, intramuscular, subcutaneous, intra-uterine or intracerebroventricular injections.

In another aspect, the present invention provides a health functional food for immune enhancement comprising Angelica extract and Angelica extract as an active ingredient.

When the composition of the present invention is used as a food additive, the composition can be added as it is or can be used together with other food or food ingredients, and can be suitably used according to a conventional method. The mixed amount of the active ingredient may be appropriately determined depending on the purpose of use (prevention, health or therapeutic treatment). In general, the composition of the present invention is added in an amount of not more than 15% by weight, preferably not more than 10% by weight based on the raw material, in the production of food or beverage. However, in the case of long-term intake for the purpose of health and hygiene or for the purpose of health control, it may be below the above range, and the active ingredient may be used in an amount above the above range because there is no problem in terms of safety.

There is no particular limitation on the kind of the food. Examples of the food to which the substance may be added include meat, sausage, bread, chocolate, candy, snacks, confectionary, pizza, ramen, other noodles, dairy products including gum, ice cream, various soups, beverages, tea, drink, Alcoholic beverages and vitamin complexes and the like, and include all of the dietary supplements in the conventional sense.

The health beverage composition of the present invention may include various flavors or natural carbohydrates, and the like as an additional ingredient, as in a general beverage. Such natural carbohydrates include monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, and natural sweeteners such as dextrin and cyclodextrin, synthetic sweeteners such as saccharine and aspartame, and the like . The proportion of natural carbohydrates is generally about 0.01 to 0.4 g, preferably about 0.02 to 0.03 g per 100 ml of the composition of the present invention.

In addition to the above, the composition of the present invention may further contain various nutrients, vitamins, electrolytes, flavors, colorants, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloid thickeners, pH adjusters, stabilizers, preservatives, glycerin, A carbonating agent used in a carbonated beverage, and the like. In addition, the composition of the present invention may include a pulp for the production of natural fruit juice, fruit juice drinks and vegetable drinks. These components may be used independently or in combination. The proportion of such additives is not critical but is generally selected in the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the composition of the present invention.

Hereinafter, preferred embodiments and production examples are provided to facilitate understanding of the present invention. However, the following Examples and Preparation Examples are provided only for the purpose of easier understanding of the present invention, and the present invention is not limited by the Examples and Production Examples.

Example  1. Angelica and Hwanggi  Preparation of extract

8 g of Angelicae gigantis Radix and 20 g of Astragali Radix were mixed, extracted and filtered for about 3 hours in distilled water, and then lyophilized under reduced pressure to prepare an extract of Angelica and Astragalus (DGBHT). Angelica and Astragalus were purchased from Daehak Oriental Pharmacy in Korea. The yield of dried extract was about 3%.

Example  2. Forced swimming model FST )

According to a known experimental method, immobility was measured during 6 minutes of forced swimming test (FST) using a 4 week old ICR male. As a result of the experiment, as shown in FIG. 1, after 10 and 14 days, the immobility time was significantly reduced in the extracts of Angelica and Astragalus (DGBHT). In particular, it showed an excellent effect compared to the group administered with the extract of Angelica or Astragalus.

In order to confirm the effects of DGBHT, the negative control group in which ICR mice were administered distilled water, the positive control group in which Chlorella vulgaris (CV) was administered at 150 mg / kg, and the experimental group in which DGBHT was administered at 100 mg / kg or 200 mg / kg The experiment was divided into four groups. Each material was administered for a total of 14 days, and the dead time was measured on the 3rd, 10th and 14th days. As shown in FIG. 2, there was no change in the negative control group, but it was confirmed that the experimental group treated with DGBHT at 200 mg / kg showed a significant change on the 10th and 14th days. In particular, it showed more excellent effect than the positive control CV.

Example  3. Blood Biochemical Analysis

Mouse blood of Example 2 was analyzed to determine the effect of DGBHT on energy sources and fatigue. As shown in FIG. 3, the concentration of Glc was significantly increased in the DGBHT-administered group, and it was confirmed that there was no change in the concentration of TP. In addition, as shown in FIG. 4, the concentration of lactate dehydrogenase (LDH) was significantly decreased in the DGBHT-administered group, and it was confirmed that there was no change in the concentration of CK (creatine kinase) and BUN (blood urea nitrogen).

Example  4. In mouse peritoneal macrophages Immune factor  And NO  Generation analysis

Non-primed cells and r-IFN-r-primed cells were treated with DGBHT to analyze the effect on the production of immune factors in mouse peritoneal macrophages. IL-12 production was analyzed by ELISA. As shown in FIG. 5, IL-12 and TNF-α production in rIFN-r-induced cells was significantly increased in the DGBHT treated group. No cytotoxicity was observed in the DGBHT treated group.

In addition, as a result of analyzing the effect of DGBHT on the production of NO, as shown in Figure 6, the treatment of DGBHT in mouse macrophages did not affect. However, primed mouse rIFN-r for 6 hours and treated with DGBHT significantly increased NO production.

In addition, based on the results of previous studies that the treatment of rIFN-r and LPS in macrophages induce iNOS and NF-kB expression, the effect of DGBHT treatment was confirmed. As shown in FIG. 7 and FIG. 8, iNOS and NF-kB were significantly increased in the DGBHT-treated group, and synergistic iNOS and NF-kB were increased in the group treated with rIFN-r and DGBHT.

Hereinafter, a pharmaceutical composition containing the composition of the present invention and a preparation of a health functional food will be described, but the present invention is not intended to be limited thereto but is specifically described.

Formulation example  1: Preparation of pharmaceutical preparation

One. Sanje  Produce

Angelica Extract and Angelica Extract 2g

1g lactose

The above components were mixed and packed in airtight bags to prepare powders.

2. Preparation of tablets

Angelica and Astragalus Extract 100mg

Corn Starch 100mg

Lactose 100mg

2 mg magnesium stearate

After mixing the above components, tablets were prepared by tableting according to a conventional method for producing tablets.

3. Preparation of Capsule

Angelica and Astragalus Extract 100mg

Corn Starch 100mg

Lactose 100mg

2 mg magnesium stearate

After mixing the above components, the capsules were filled in gelatin capsules according to the conventional preparation method of capsules.

Formulation example  2 ; Manufacture of food

1. Preparation of dietary supplements

Angelica and Astragalus Extract 100 mg

Vitamin mixture quantity

Vitamin A acetate 70 μg

Vitamin E 1.0 mg

Vitamin B1 0.13 mg

Vitamin B2 0.15 mg

Vitamin B6 0.5 mg

Vitamin B12 0.2 μg

Vitamin C 10 mg

Biotin 10 μg

Nicotinamide 1.7 mg

Folic acid 50 μg

Calcium pantothenate 0.5 mg

Mineral mixture

Ferrous Sulfate 1.75 mg

Zinc Oxide 0.82 mg

Magnesium carbonate 25.3 mg

15 mg potassium monophosphate

Dicalcium Phosphate 55 mg

Potassium Citrate 90 mg

Calcium Carbonate 100 mg

Magnesium chloride 24.8 mg

Although the composition ratio of the above-mentioned vitamin and mineral mixture is comparatively mixed with a composition suitable for health food as a preferred embodiment, the compounding ratio may be arbitrarily modified, and the above ingredients are mixed according to a conventional method for producing healthy foods , Granules can be prepared and used in the manufacture of health food compositions according to conventional methods.

2. Health drink  Produce

Angelica and Astragalus Extract 100 mg

15 g of vitamin C

Vitamin E (powder) 100 g

19.75 g of ferrous lactate

3.5 g of zinc oxide

Nicotinic acid amide 3.5 g

Vitamin A 0.2 g

Vitamin B1 0.25 g

Vitamin B2 0.3g

Water quantification

The above components were mixed according to a conventional health drink manufacturing method, and the mixture was stirred and heated at 85 DEG C for about 1 hour. The solution thus prepared was filtered and sterilized in a sterilized 2 liter container, It is used in the production of the health beverage composition of the invention.

Although the compositional ratio is relatively mixed with a component suitable for a favorite drink, it is also possible to arbitrarily modify the compounding ratio according to the regional or national preference such as the demand class, the demanding country, and the use purpose.

Claims (6)

Immunity-enhancing pharmaceutical composition comprising Angelica extract and Astragalus extract as an active ingredient. The composition of claim 1, wherein the extract is a water extract. According to claim 1, wherein the extract is a composition characterized in that the mixing ratio of Angelica and Astragalus 2: 5. Pharmaceutical composition for preventing or treating autoimmune diseases, including Angelica gigas and Astragalus extract as active ingredients. The method of claim 4, wherein the immune disease is cold, asthma, pneumonia, allergic rhinitis, allergic conjunctivitis, atopic dermatitis, allergy, rheumatoid arthritis, Alzheimer's disease, autoimmune thyroid disease, inflammatory bowel disease, aplastic anemia, erythema A composition characterized in that it is selected from the group consisting of reflective lupus and psoriasis. Health functional food for immune enhancement comprising Angelica extract and Angelica extract as active ingredients.

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