KR102416743B1 - Composition comprising fermented germinating plant extract and preparation method thereof - Google Patents
Composition comprising fermented germinating plant extract and preparation method thereof Download PDFInfo
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- KR102416743B1 KR102416743B1 KR1020200092148A KR20200092148A KR102416743B1 KR 102416743 B1 KR102416743 B1 KR 102416743B1 KR 1020200092148 A KR1020200092148 A KR 1020200092148A KR 20200092148 A KR20200092148 A KR 20200092148A KR 102416743 B1 KR102416743 B1 KR 102416743B1
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- fermented
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- germinated plant
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Abstract
본 발명은 발아식물을 발효하여 얻은 추출물을 포함하는 조성물 및 그 제조방법에 관한 것이다.
본 발명에 따르면, 발아식물이 가지는 유효성분의 함량을 증가시키는 동시에 세포에 유해한 독성 성분을 최소화하여 유익한 효과를 증대시킬 수 있다. 또한, 항산화 또는 항염 효과가 향상된 천연물질로서 다양한 분야에 용이하게 적용할 수 있다.The present invention relates to a composition comprising an extract obtained by fermenting a germinated plant and a method for preparing the same.
According to the present invention, it is possible to increase the beneficial effect by increasing the content of the active ingredient of the germinating plant and at the same time minimizing the toxic ingredient harmful to the cell. In addition, as a natural substance with improved antioxidant or anti-inflammatory effects, it can be easily applied to various fields.
Description
본 발명은 발아식물을 발효하여 얻은 추출물을 포함하는 조성물에 관한 것이다.The present invention relates to a composition comprising an extract obtained by fermenting a germinated plant.
또한, 본 발명은 상기 조성물을 제조하는 방법에 관한 것이다.The present invention also relates to a method for preparing said composition.
식물의 씨앗 내에는 생명의 유지에 필요한 영양소가 충분하다. 또한, 식물을 비롯한 생명체는 보통 싹이 트거나 성장하는 어린 시기에 성장력이 가장 왕성하다. 이 때 성장에 필요한 물질들이 생합성되며, 발아 시기에는 비타민, 미네랄 등의 유효성분 함량이 일반 성체의 최소 3배 이상이다.Plant seeds contain enough nutrients to sustain life. In addition, living things, including plants, usually have the most vigorous growth during the budding or growth period. At this time, the substances necessary for growth are biosynthesized, and the content of active ingredients such as vitamins and minerals is at least three times higher than that of a general adult during the germination period.
식물의 싹이 발아할 때 생합성되는 새로운 물질들은 인체에 유익한 효과를 주는 성분들을 많이 함유하지만, 그 중 독성 성분이 포함되는 경우가 있다. 발아 시 생성되는 독성 성분은 인체에 유해한 영향을 줄 수 있으며, 예를 들면 감자, 가지, 토마토 등의 식물에 있어서 독성 성분이 생성될 수 있으므로 주의가 필요하다.New substances biosynthesized when plant buds germinate contain many components that have beneficial effects on the human body, but some of them contain toxic components. Toxic components generated during germination may have a harmful effect on the human body, and for example, in plants such as potatoes, eggplants and tomatoes, toxic components may be generated, so caution is required.
종래에는 새싹 또는 발아식물들이 포함하는 유효성분들의 효과가 알려지며 이를 적용한 제품들이 제조되고 있다.Conventionally, the effects of active ingredients contained in sprouts or germinated plants are known, and products to which they are applied are being manufactured.
그러나, 발아식물의 함량을 증가시키는 경우 이들이 가지는 독성 성분으로 인하여 세포가 유해한 영향을 받을 수 있으며, 예를 들면 배탈, 설사, 알러지 등이 유발될 수 있다.However, when the content of germinated plants is increased, cells may be adversely affected due to their toxic components, and for example, stomach upset, diarrhea, allergies, etc. may be induced.
그러므로, 발아식물이 가지는 유효성분의 효능을 극대화하면서 독성에 대한 안전성을 향상시키기 위한 연구가 필요하다.Therefore, there is a need for research to improve safety against toxicity while maximizing the efficacy of active ingredients of germinated plants.
본 발명의 목적은 발아식물을 발효하여 얻은 추출물을 포함하는 조성물을 제공하는 것이다.It is an object of the present invention to provide a composition comprising an extract obtained by fermenting a germinated plant.
본 발명의 다른 목적은 상기 조성물을 제조하는 방법을 제공하는 것이다.Another object of the present invention is to provide a method for preparing the composition.
상기 과제를 해결하기 위하여, 본 발명은In order to solve the above problems, the present invention
발아식물에 미생물을 접종하여 발효물을 제조하는 단계; 및preparing a fermented product by inoculating a germinating plant with microorganisms; and
상기 발효물을 용매 추출하여 추출물을 수득하는 단계를 포함하는, 발아식물 발효 추출물을 포함하는 조성물의 제조방법을 제공한다.It provides a method for preparing a composition comprising a germinated plant ferment extract, comprising the step of obtaining an extract by solvent extraction of the fermented product.
일구현예에 따르면, 상기 발아식물은 카뮤트(Kamut) 새싹, 새싹귀리, 밀크시슬 새싹, 알팔파(alfalfa) 새싹 및 브로콜리 새싹으로 이루어지는 군으로부터 선택되는 하나 이상을 포함할 수 있다.According to one embodiment, the germinated plant may include one or more selected from the group consisting of kamut sprouts, sprout oats, milk thistle sprouts, alfalfa sprouts, and broccoli sprouts.
일구현예에 따르면, 상기 미생물은 Saccharomyces sp., Lactobacillus sp. 또는 이들의 조합을 포함할 수 있다.According to one embodiment, the microorganism is Saccharomyces sp. , Lactobacillus sp. or a combination thereof.
일구현예에 따르면, 상기 발효물을 제조하는 단계는 상기 발아식물에 상기 미생물을 1일 1 내지 5회 접종하여 4 내지 10일 동안 발효하는 단계를 포함할 수 있다.According to one embodiment, preparing the fermented product may include inoculating the germinating plant with the
일구현예에 따르면, 상기 발효물을 제조하는 단계는 상기 발아식물에 Saccharomyces sp.를 1일 1 내지 5회 접종하여 2 내지 5일 동안 발효하는 단계; 및According to one embodiment, the step of preparing the fermented product is Saccharomyces sp. Inoculating 1 to 5 times a day and fermenting for 2 to 5 days; and
Lactobacillus sp.를 1일 1 내지 5회 접종하여 2 내지 5일 동안 발효하는 단계를 순차적으로 포함할 수 있다. Lactobacillus sp. may include sequentially inoculating 1 to 5 times a day and fermenting for 2 to 5 days.
일구현예에 따르면, 상기 발효물을 제조하는 단계는 상기 발아식물에 Saccharomyces sp. 및 Lactobacillus sp.를 2:1 내지 10:1 중량비로 접종하는 단계를 포함할 수 있다.According to one embodiment, the step of preparing the fermented product is Saccharomyces sp. and Lactobacillus sp. may include the step of inoculating a 2:1 to 10:1 weight ratio.
일구현예에 따르면, 상기 용매는 물, 에탄올 또는 이들의 조합을 포함할 수 있다.According to one embodiment, the solvent may include water, ethanol, or a combination thereof.
일구현예에 따르면, 상기 추출물을 수득하는 단계는 상기 발아식물에 5 내지 20중량 배수의 용매를 첨가하고, 10초 내지 12시간 동안 교반하는 단계를 포함할 수 있다.According to one embodiment, obtaining the extract may include adding a solvent of 5 to 20 weight multiples to the germinating plant, and stirring for 10 seconds to 12 hours.
일구현예에 따르면, 본 발명에 따른 제조방법은 상기 추출물을 농축하여 분말을 제조하는 단계를 더 포함할 수 있다.According to one embodiment, the manufacturing method according to the present invention may further include the step of preparing a powder by concentrating the extract.
본 발명의 다른 구현예에 따르면, 상기한 바와 같은 방법으로 제조된, 발아식물 발효 추출물을 포함하는 조성물을 제공한다.According to another embodiment of the present invention, there is provided a composition comprising a germinated plant ferment extract prepared by the method as described above.
일구현예에 따르면, 상기 조성물은 항염 또는 항산화용으로 적용될 수 있다.According to one embodiment, the composition may be applied for anti-inflammatory or antioxidant.
본 발명의 또 다른 구현예에 따르면, 상기 조성물을 포함하는 항염 또는 항산화용 화장료 조성물을 제공한다.According to another embodiment of the present invention, there is provided a cosmetic composition for anti-inflammatory or antioxidant comprising the composition.
또한, 본 발명의 다른 구현예에 따르면, 상기 조성물을 포함하는 항염 또는 항산화용 건강 기능성 식품 조성물을 제공한다.In addition, according to another embodiment of the present invention, there is provided a health functional food composition for anti-inflammatory or antioxidant comprising the composition.
또한, 본 발명의 다른 구현예에 따르면, 상기 조성물을 포함하는 항염 또는 항산화용 약학 조성물을 제공한다.In addition, according to another embodiment of the present invention, there is provided a pharmaceutical composition for anti-inflammatory or antioxidant comprising the composition.
기타 본 발명에 따른 구현예들의 구체적인 사항은 이하의 상세한 설명에 포함되어 있다.The specific details of other embodiments according to the present invention are included in the detailed description below.
본 발명에 따르면, 발아식물의 유효성분 함량을 증가시키는 동시에 독성을 최소화하여 세포에 유익한 효과를 증대시킬 수 있다.According to the present invention, it is possible to increase the beneficial effect on cells by increasing the content of the active ingredient of the germinating plant and at the same time minimizing the toxicity.
또한, 항산화 또는 항염 효과가 향상된 천연물질로서 다양한 분야에 용이하게 적용할 수 있다.In addition, as a natural substance with improved antioxidant or anti-inflammatory effects, it can be easily applied to various fields.
도 1은 세포독성을 나타내는 그래프이다.
도 2는 유효성분의 함량 변화를 나타내는 그래프이다.
도 3은 활성산소 소거능을 나타내는 그래프이다.
도 4 및 5는 염증 유발 물질의 발현 정도를 나타내는 그래프이다.1 is a graph showing cytotoxicity.
Figure 2 is a graph showing the change in the content of the active ingredient.
3 is a graph showing the active oxygen scavenging ability.
4 and 5 are graphs showing the level of expression of inflammatory substances.
본 발명은 다양한 변환을 가할 수 있고 여러 가지 실시예를 가질 수 있는 바, 특정 실시예를 도면에 예시하고 상세한 설명에 상세하게 설명하고자 한다. 그러나, 이는 본 발명을 특정한 실시 형태에 대해 한정하려는 것이 아니며, 본 발명의 사상 및 기술 범위에 포함되는 모든 변환, 균등물 내지 대체물을 포함하는 것으로 이해되어야 한다. 본 발명을 설명함에 있어서 관련된 공지 기술에 대한 구체적인 설명이 본 발명의 요지를 흐릴 수 있다고 판단되는 경우 그 상세한 설명을 생략한다.Since the present invention can apply various transformations and can have various embodiments, specific embodiments are illustrated in the drawings and described in detail in the detailed description. However, this is not intended to limit the present invention to specific embodiments, and should be understood to include all modifications, equivalents, and substitutes included in the spirit and scope of the present invention. In describing the present invention, if it is determined that a detailed description of a related known technology may obscure the gist of the present invention, the detailed description thereof will be omitted.
본 명세서 내에서 특별한 언급이 없는 한, "내지"라는 표현은 해당 수치를 포함하는 표현으로 사용된다. 구체적으로 예를 들면, "1 내지 2"라는 표현은 1 및 2를 포함할 뿐만 아니라 1과 2 사이의 수치를 모두 포함하는 것을 의미한다.Unless otherwise specified in the present specification, the expression "between" is used as an expression including the corresponding numerical value. Specifically, for example, the expression "1 to 2" is meant to include all numbers between 1 and 2 as well as 1 and 2.
이하, 본 발명의 구현예에 따른 발아식물 발효 추출물을 포함하는 조성물 및 그 제조방법에 대하여 보다 상세하게 설명한다.Hereinafter, a composition comprising a fermented germinated plant extract according to an embodiment of the present invention and a method for preparing the same will be described in more detail.
근래에는 발아식물의 유익한 효능이 알려지면서 이를 다양한 제품에 적용하려는 시도가 계속되고 있다. 식물은 발아를 위하여 필요한 많은 유효성분을 생합성한다. 하지만, 이 때, 곰팡이, 박테리아 등과 같은 외부 환경으로부터의 방어를 위하여 독성 성분 또한 생합성할 수 있다.In recent years, as the beneficial effects of germinated plants are known, attempts to apply them to various products are continuing. Plants biosynthesize many active ingredients necessary for germination. However, at this time, toxic components may also be biosynthesized for defense from external environments such as mold and bacteria.
그러므로, 본 발명에서는 발아식물이 가지는 유효성분을 극대화하고, 독성 성분을 최소화하기 위한 조성물 및 그 제조방법을 제공한다.Therefore, the present invention provides a composition for maximizing the active ingredient of the germinating plant and minimizing the toxic ingredient and a method for preparing the same.
구체적으로, 본 발명은 발아식물에 미생물을 접종하여 발효물을 제조하는 단계; 및Specifically, the present invention comprises the steps of preparing a fermented product by inoculating a germinating plant with microorganisms; and
상기 발효물을 용매 추출하여 추출물을 수득하는 단계를 포함하는, 발아식물 발효 추출물을 포함하는 조성물의 제조방법을 제공한다.It provides a method for preparing a composition comprising a germinated plant ferment extract, comprising the step of obtaining an extract by solvent extraction of the fermented product.
일구현예에 따르면, 상기 발아식물은 종자(씨앗)를 1일 내지 10일 동안 생장시킨 식물을 포함할 수 있다. 예를 들면, 카뮤트(Kamut) 새싹, 새싹귀리, 밀크시슬 새싹, 알팔파(alfalfa) 새싹 및 브로콜리 새싹으로 이루어지는 군으로부터 선택되는 하나 이상을 포함할 수 있다.According to one embodiment, the germinating plant may include a plant in which seeds (seeds) are grown for 1 to 10 days. For example, it may include one or more selected from the group consisting of kamut sprouts, sprout oats, milk thistle sprouts, alfalfa sprouts, and broccoli sprouts.
카뮤트(Kamut)는 이집트에서 재배하기 시작한 고대 원시 곡물 중 하나이다. 또한, 호라산 밀(Khorasan Wheat)의 한 종류로서 일반 밀보다 2배 정도 큰 크기이며 단백질 함량이 높다. 식이섬유, 미네랄, 마그네슘, 아연, 철분, 비타민 B군 및 셀레늄 등의 유효성분을 포함한다. 다만, 과하게 섭취하는 경우 구토, 설사, 탈모 등의 부작용이 발생할 수 있다.Kamut is one of the ancient primitive grains that began to be cultivated in Egypt. In addition, as a type of Khorasan wheat, it is twice as large as normal wheat and has a high protein content. Contains active ingredients such as dietary fiber, minerals, magnesium, zinc, iron, vitamin B group and selenium. However, if consumed in excess, side effects such as vomiting, diarrhea, and hair loss may occur.
새싹귀리는 귀리를 발아시켜 수확한 새싹으로, 베타글루칸, 아미노산, 폴리페놀, 엽록소, 칼슘, 비타민 B 등의 유효성분을 포함한다. 다만, 과다 섭취 시 복통, 설사, 복부팽만, 신장질환의 부작용이 발생할 수 있다.Sprout oats are sprouts harvested by germinating oats, and contain active ingredients such as beta-glucan, amino acids, polyphenols, chlorophyll, calcium, and B vitamins. However, if consumed in excess, side effects such as abdominal pain, diarrhea, abdominal distension, and kidney disease may occur.
밀크시슬(milk thistle)은 잎, 줄기, 뿌리, 씨앗의 모든 부분을 식용할 수 있고, 특히 열매 및 씨앗에는 플라보노리그난을 함유되어 있고, 전체 주요 구성 성분으로는 실리마린을 포함한다. 실리마린은 간세포의 세포막을 보호하여 간 기능을 개선한다고 알려져 있다. 다만, 과다 섭취 시 구역질, 가스, 설사 등이 발생할 수 있다.Milk thistle (milk thistle) is edible all parts of the leaves, stems, roots and seeds, especially the fruits and seeds contain flavonolignans, and the whole main component contains silymarin. Silymarin is known to improve liver function by protecting the cell membrane of hepatocytes. However, if consumed in excess, nausea, gas, and diarrhea may occur.
알팔파 새싹은 콩과 다년생초본으로써 비타민 A,B,C,D,K,U 등 포함되어 있고, 사포닌과 베타카로틴, 아르기닌, 파이토에스트로겐 등 유효성분을 함유하고 있다. 다만, 과다 섭취 시 위장장애, 설사 등을 유발할 수 있다.Alfalfa sprout is a soybean and perennial herb and contains vitamins A, B, C, D, K, U, etc., and contains active ingredients such as saponin, beta-carotene, arginine, and phytoestrogens. However, excessive intake may cause gastrointestinal disturbances and diarrhea.
브로콜리 새싹은 브로콜리 씨앗을 4~6일 정도 생장시킨 것이다. 다 자란 브로콜리에 비하여 설포라판(sulforaphane)이 풍부하고, 비타민 C, 비타민 A, 비타민 B, 칼륨, 인, 칼슘 등의 유효성분을 포함한다. 다만, 과다 복용 시 위장장애, 설사, 복통의 부작용이 발생할 수 있다.Broccoli sprouts are broccoli seeds grown for 4-6 days. Compared to mature broccoli, it is rich in sulforaphane and contains active ingredients such as vitamin C, vitamin A, vitamin B, potassium, phosphorus, and calcium. However, side effects such as gastrointestinal disturbances, diarrhea and abdominal pain may occur when taking an overdose.
본 발명은 발아식물이 가지는 독성 성분으로 인한 부작용을 최소화하기 위하여 미생물을 이용한다. 미생물은 예를 들면, Saccharomyces sp., Lactobacillus sp. 또는 이들의 조합을 포함할 수 있다. 1종 이상의 미생물은 발아식물에 동시에 또는 각각 순차적으로 접종할 수 있다.The present invention uses microorganisms to minimize side effects due to toxic components of germinating plants. Microorganisms are, for example, Saccharomyces sp. , Lactobacillus sp. or a combination thereof. The one or more microorganisms may be inoculated simultaneously or sequentially with each germinating plant.
일구현예에 따르면, 상기 발효물을 제조하는 단계는 발아식물에 미생물을 1일 1 내지 5회, 예를 들면 1일 1 내지 3회 접종하여, 4 내지 10일, 예를 들면 5 내지 7일 동안 발효하는 단계를 포함할 수 있다.According to one embodiment, the step of preparing the fermented product is inoculated with a
구체적으로 예를 들면, 발아식물에 1종 이상의 미생물을 동시에 1일 1 내지 5회 접종하고, 4 내지 10일 동안 발효하여 발효물을 제조할 수 있다. 또한, 예를 들면 발아식물에 Saccharomyces sp.를 1일 1 내지 5회, 예를 들면 1 내지 3회 접종하여 2 내지 5일, 예를 들면 3 내지 4일 동안 발효한 후, Lactobacillus sp.를 1일 1 내지 5회, 예를 들면 1 내지 3회 접종하여 2 내지 5일, 예를 들면 3 내지 4일 동안 발효하여 발효물을 제조할 수 있다. 이 때, Saccharomyces sp. 처리량은 1 X 107 ~ 1 X 1012 CFU/ml, 예를 들면 1 X 1010 ~ 1X1012 CFU/ml 농도로 1일 10% 내지 80%일 수 있고, Lactobacillus sp. 처리량은 1 X 107 ~ 1 X 1012 CFU/ml. 예를 들면 1 X 1010 ~ 1 X 1012 CFU/ml 농도로 1일 5% 내지 10%일 수 있다.Specifically, for example, germinating plants may be simultaneously inoculated with one or
또한, 예를 들면 발아식물에 Lactobacillus sp.를 1일 1 내지 5회, 예를 들면 1 내지 3회 접종하여 접종하여 2 내지 5일, 예를 들면 3 내지 4일 동안 발효한 후, Saccharomyces sp.를 1일 1 내지 5회, 예를 들면 1 내지 3회 접종하여 2 내지 5일, 예를 들면 3 내지 4일 동안 발효하여 발효물을 제조할 수 있다.In addition, for example, in germinating plants, Lactobacillus sp. 1 to 5 times a day, for example, inoculated by
일구현예에 따르면, 상기 발효물을 제조하는 단계는 상기 발아식물에 Saccharomyces sp. 및 Lactobacillus sp.를 접종하는 단계를 포함할 수 있다. 이 때, 접종한 Lactobacillus sp. 총 중량에 대한 Saccharomyces sp. 총 중량은 2 이상일 수 있다. 또한, 예를 들면, Saccharomyces sp. 및 Lactobacillus sp.의 중량비는 2:1 내지 10:1, 예를 들면 3:1 내지 5:1일 수 있다.According to one embodiment, the step of preparing the fermented product is Saccharomyces sp. and Lactobacillus sp. It may include the step of inoculation. At this time, the inoculated Lactobacillus sp. Saccharomyces sp. for total weight. The total weight may be two or more. Also, for example, Saccharomyces sp. and Lactobacillus sp. The weight ratio may be 2:1 to 10:1, for example 3:1 to 5:1.
일구현예에 따르면, 상기 발효물을 열수 추출, 에탄올(주정)추출 또는 이들 조합의 방법으로 추출하여 추출물을 수득할 수 있다. 예를 들면, 상기 발효물로부터 에탄올 추출하여 추출물을 제조할 수 있다.According to one embodiment, the extract can be obtained by extracting the fermented product by hot water extraction, ethanol (alcohol) extraction, or a combination thereof. For example, an extract may be prepared by ethanol extraction from the fermented product.
용매로 사용되는 물은 증류수를 사용할 수 있으나, 화장료, 식품 또는 의약품에 사용할 수 있는 정도의 것이라면 특별히 제한되지 않는다. 또한, 에탄올 추출 용매로는 예를 들면, 70%(v/v) 농도의 에탄올을 사용하는 것이 바람직하다.Distilled water may be used as the water used as the solvent, but it is not particularly limited as long as it can be used for cosmetics, food, or pharmaceuticals. In addition, it is preferable to use, for example, 70% (v/v) concentration of ethanol as the ethanol extraction solvent.
일구현예에 따르면, 상기 추출물을 수득하는 단계는 상기 발아식물에 5 내지 20중량 배수, 예를 들면 5 내지 15 또는 5 내지 10 중량 배수의 용매를 첨가하고, 10초 내지 12시간, 예를 들면 1시간 내지 4시간 동안 교반하는 단계를 포함할 수 있다.According to one embodiment, the step of obtaining the extract is 5 to 20 weight multiples, for example, 5 to 15 or 5 to 10 weight multiples of a solvent added to the germinating plant, 10 seconds to 12 hours, for example It may include stirring for 1 hour to 4 hours.
일구현예에 따르면, 본 발명은 상기 추출물을 정제하는 단계를 더 포함할 수 있다. 정제방법으로는 원심분리, 필터 등 당업계에서 일반적으로 사용하는 방법이라면 특별히 제한되지는 않는다.According to one embodiment, the present invention may further include the step of purifying the extract. The purification method is not particularly limited as long as it is a method generally used in the art, such as centrifugation or a filter.
또한, 액상 상태의 추출물을 농축하여 분말을 제조하는 단계를 추가로 포함할 수 있다. 농축은 가열, 감압, 통풍, 냉동, 분무, 삼투압, 역삼투압 방법 등을 적용할 수 있으나, 상기한 방법에 특별히 한정되지는 않으며, 예를 들면, 회전증발농축기를 사용할 수 있다.In addition, the step of concentrating the extract in a liquid state to prepare a powder may be further included. Concentration may be performed by heating, decompression, ventilation, freezing, spraying, osmosis, reverse osmosis, and the like, but is not particularly limited to the above-described method, and for example, a rotary evaporator may be used.
가열농축은 농축 대상을 가열하여 용매만을 기체로 증발시키는 방법이다. 감압농축은 감압 하에서 진행하여 농축 속도를 향상시킬 수 있다. 통풍농축은 실온 또는 고온에서 기체와 액체의 계면에 기체를 가하여 농축하는 방법이다. 냉동농축은 열에 약한 용질에 적용하기 용이한 것으로, 농축 대상을 냉각하여 고체상이 된 용매를 석출하는 방법이다. 분무농축은 농축 대상을 압출하여 표면적을 증대시키는 것으로, 가열 또는 감압을 함께 적용하여 급속한 농축을 진행할 수 있다. 삼투압 또는 역삼투압 농축은 반투막을 이용하여 농축 대상이 가지는 삼투압 이상의 압력을 가하여 통과시켜 분리하는 방법이다.Heat concentration is a method in which only the solvent is evaporated into a gas by heating the object to be concentrated. Concentration under reduced pressure may be performed under reduced pressure to improve the concentration rate. Gout concentration is a method of concentration by adding gas to the interface between gas and liquid at room temperature or high temperature. Freezing concentration is easy to apply to a solute weak to heat, and is a method of cooling a concentration target to precipitate a solid solvent. Spray concentration is to increase the surface area by extruding the object to be concentrated, and rapid concentration can be carried out by applying heating or reduced pressure together. Osmotic pressure or reverse osmosis concentration is a method of separating by passing a pressure higher than the osmotic pressure of the object to be concentrated using a semi-permeable membrane.
본 발명의 다른 구현예에 따르면, 상기한 바와 같은 방법으로 제조된, 발아식물 발효 추출물을 포함하는 조성물을 제공한다.According to another embodiment of the present invention, there is provided a composition comprising a germinated plant ferment extract prepared by the method as described above.
일구현예에 따르면, 본 발명에 따른 조성물은 발아식물 원재료에 비하여 항산화 물질의 함량을 월등히 향상시킬 수 있다. 항산화 물질로는 대표적으로 폴리페놀(polyphenol)과 같은 성분을 포함할 수 있다. 폴리페놀은 활성 산소를 저해하여 항산화 효과를 가지며, 노화를 방지한다. 또한, 활성 산소에 노출되어 손상되는 DNA 보호, 세포 구성 단백질 및 효소를 보호하는 기능이 뛰어나 다양한 질병에 대한 위험도를 낮춘다고 알려져 있다. 더불어 항암 작용과 함께 심장 질환을 막아주는 역할을 한다.According to one embodiment, the composition according to the present invention can significantly improve the content of antioxidants compared to the raw material of germinated plants. Antioxidants may include components such as polyphenols. Polyphenols have antioxidant effects by inhibiting free radicals and prevent aging. In addition, it is known that it has excellent functions in protecting DNA, cell constituent proteins and enzymes, which are damaged by exposure to reactive oxygen species, thereby lowering the risk of various diseases. In addition, it acts as an anti-cancer agent and prevents heart disease.
일구현예에 따르면, 본 발명의 조성물은 항염 또는 항산화 용도로 사용될 수 있다.According to one embodiment, the composition of the present invention may be used for anti-inflammatory or antioxidant purposes.
또한, 본 발명의 조성물은 통상적인 화장료 조성물, 피부 외용제 약학 조성물 등 피부학적으로 적용이 가능한 어떠한 제형으로도 제조될 수 있다. 피부학적으로 적용이 가능하다는 것은 적용 대상에게 비교적 비독성이고 무해한 유효작용을 할 수 있는 조성물로서, 피부에 적용할 수 있는 외용제를 포함할 수 있으며, 상기 조성물로부터 기인하는 부작용이 유효 성분의 효능을 저하시키기 않고, 적용 대상에 심각한 자극을 유발하지 않으면서 유효성분들의 활성 및 물성을 손상시키지 않는다는 것을 의미한다.In addition, the composition of the present invention can be prepared in any formulation that can be applied dermatologically, such as a conventional cosmetic composition, a pharmaceutical composition for external application to the skin, and the like. Dermatologically applicable is a composition that can have a relatively non-toxic and harmless effective action to the subject, and may include an external application that can be applied to the skin. It means that the activity and physical properties of the active ingredients are not impaired without deteriorating, causing serious irritation to the application target.
또한, 본 발명의 피부 외용제 조성물은 당업계에서 통상적으로 제조되는 어떠한 제형으로도 제조될 수 있으며, 예를 들어, 용액, 현탁액, 유액, 유탁액, 페이스트, 겔, 팩, 크림, 로션, 파우더, 비누, 계면활성제-함유 클렌징, 오일, 분말 파운데이션, 유탁액 파운데이션, 왁스 파운데이션, 스프레이 및 모발 화장료 등으로 제형화될 수 있으나, 이에 한정되는 것은 아니다. In addition, the composition for external application for skin of the present invention may be prepared in any formulation conventionally prepared in the art, for example, a solution, suspension, emulsion, emulsion, paste, gel, pack, cream, lotion, powder, It may be formulated into soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, spray, and hair cosmetics, but is not limited thereto.
구체적으로, 상기 화장료 조성물은 스킨로션, 스킨소프너, 스킨토너, 아스트린젠트, 로션, 밀크로션, 모이스쳐 로션, 영양로션, 맛사지크림, 영양크림, 모이스처크림, 핸드크림, 파운데이션, 에센스, 영양에센스, 팩, 비누, 헤어샴푸, 풋샴푸, 클렌징폼, 클렌징로션, 클렌징크림, 바디로션 및 바디클린저의 제형 등으로 제조될 수 있다.Specifically, the cosmetic composition is a skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nourishing lotion, massage cream, nourishing cream, moisture cream, hand cream, foundation, essence, nourishing essence, pack, It can be prepared in the formulation of soap, hair shampoo, foot shampoo, cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser.
본 발명의 또 다른 구현예에 따르면, 상기한 바와 같은 조성물을 포함하는 기능성 식품 조성물을 제공할 수 있다. 예를 들면, 상기 기능성 식품은 영양제, 비타민, 광물(전해질), 합성 풍미제 및 천연 풍미제 등의 풍미제, 착색제 및 중진제(치즈, 초콜릿 등), 펙트산 및 그의 염, 알긴산 및 그의 염, 유기산, 보호성 콜로이드 증점제, pH 조절제, 안정화제, 방부제, 글리세린, 알콜, 탄산 음료에 사용되는 탄산화제 등을 포함할 수 있다. 또한, 천연 과일 쥬스 및 야채 음료의 제조를 위한 과육을 포함할 수 있다.According to another embodiment of the present invention, it is possible to provide a functional food composition comprising the composition as described above. For example, the functional food may include nutrients, vitamins, minerals (electrolytes), synthetic flavoring agents and flavoring agents such as natural flavoring agents, coloring agents and thickening agents (cheese, chocolate, etc.), pectic acid and salts thereof, alginic acid and salts thereof , organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like. It may also contain pulp for the production of natural fruit juices and vegetable beverages.
일구현예에 따르면, 상기 기능성 식품은 충진제, 증량제, 결합제, 습윤제, 붕해제, 계면활성제, 희석제, 부형제 등을 첨가하여 제제화할 수 있다. 상기 담체, 부형제, 희석제 및 첨가제의 구체적인 예로는 이에 한정하는 것은 아니나, 락토즈, 덱스트로즈, 슈크로즈, 솔비톨, 만니톨, 에리스리톨, 전분, 아카시아 고무, 인산칼슘, 알지네이트, 젤라틴, 칼슘 포스페이트, 칼슘 실리케이트, 미세결정성 셀룰로즈, 폴리비닐피롤리돈, 셀룰로즈, 폴리비닐피롤리돈, 메틸셀룰로즈, 물, 설탕시럽, 메틸셀룰로즈, 메틸 하이드록시 벤조에이트, 프로필하이드록시 벤조에이트, 활석, 스테아트산 마그네슘 및 미네랄 오일로 이루어지는 그룹으로부터 선택되는 하나 이상을 포함할 수 있다.According to one embodiment, the functional food may be formulated by adding a filler, an extender, a binder, a wetting agent, a disintegrant, a surfactant, a diluent, an excipient, and the like. Specific examples of the carrier, excipient, diluent and additive include, but are not limited to, lactose, dextrose, sucrose, sorbitol, mannitol, erythritol, starch, acacia gum, calcium phosphate, alginate, gelatin, calcium phosphate, calcium Silicate, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, polyvinylpyrrolidone, methylcellulose, water, sugar syrup, methylcellulose, methyl hydroxy benzoate, propyl hydroxy benzoate, talc, magnesium stearate And it may include one or more selected from the group consisting of mineral oil.
일구현예에 따르면, 본 발명에 따른 약학 조성물은 약제적으로 허용 가능한 담체를 포함할 수 있다. 약제학적으로 허용되는 담체는 결합제, 활탁제, 붕해제, 부형제, 윤활제, 가용화제, 분산제, 안정화제, 현탁화제, 색소, 향료, 완충제, 보존제, 무통화제, 가용화제, 등장제 등을 혼합하여 사용할 수 있다. 본 발명의 약학 조성물의 제형은 상술한 바와 같은 약제학적으로 허용되는 담체와 혼합하여 다양하게 제조될 수 있다. 본 발명에 따른 약학 조성물의 제형은 용액, 현탁액, 연고, 정제, 캡슐 등으로 제형화 할 수 있으나, 상기한 바에 한정하지는 않는다.According to one embodiment, the pharmaceutical composition according to the present invention may include a pharmaceutically acceptable carrier. Pharmaceutically acceptable carriers include binders, lubricants, disintegrants, excipients, lubricants, solubilizers, dispersants, stabilizers, suspending agents, pigments, fragrances, buffers, preservatives, softening agents, solubilizers, isotonic agents, etc. can be used The dosage form of the pharmaceutical composition of the present invention can be prepared in various ways by mixing with a pharmaceutically acceptable carrier as described above. The formulation of the pharmaceutical composition according to the present invention may be formulated as a solution, suspension, ointment, tablet, capsule, etc., but is not limited thereto.
이하, 본 발명이 속하는 기술 분야에서 통상의 지식을 가진 자가 용이하게 실시할 수 있도록 본 발명의 실시예에 대하여 상세히 설명한다. 그러나 본 발명은 여러 가지 상이한 형태로 구현될 수 있으며 여기에서 설명하는 실시예에 한정되지 않는다.Hereinafter, embodiments of the present invention will be described in detail so that those of ordinary skill in the art can easily carry out the present invention. However, the present invention may be embodied in several different forms and is not limited to the embodiments described herein.
실시예Example
카뮤트(Kamut®) 종자를 4 내지 6일 동안 생장시켜 싹을 틔운 후, 건조된 카뮤트 새싹을 분말로 제조하였다. 카뮤트 새싹 분말은 상업적으로 입수 가능한 것으로 대체할 수 있다.Kamut ® seeds were grown for 4 to 6 days to germinate, and dried kamut sprouts were prepared as powder. The kamut sprout powder can be substituted with commercially available ones.
Saccharomyces cerevisiae(사카로미세스 세레비시아, KCTC7296, 생물자원센터)를 YM배지에 1 내지 3일 배양하여 용액을 제조하였다.Saccharomyces cerevisiae (Saccharomyces cerevisiae, KCTC7296, Center for Biological Resources) was cultured in YM medium for 1 to 3 days to prepare a solution.
또한, Lactobacillus sakei(락토바실러스 사케이, KCTC3802, 생물자원센터)를 MRS배지에 1 내지 3일 배양하여 용액을 제조하였다.In addition, Lactobacillus sakei (Lactobacillus sakei, KCTC3802, Center for Biological Resources) was cultured in MRS medium for 1 to 3 days to prepare a solution.
카뮤트 새싹 분말에 먼저 Saccharomyces sp. 용액을 3일 동안, 1일 1 내지 3회 분무 접종하여 발효하였다. 그 후, Lactobacillus sp. 용액을 3일 동안, 1일 1 내지 3회 분무 접종하여 발효하여 발효물을 제조하였다. 이 때, 접종한 Saccharomyces sp. 용액은 1 X 1010 ~ 1 X 1012 CFU/ml 농도로 1일 10% 내지 80% 양이고, Lactobacillus sp.용액은 1 X 1010 ~ 1 X 1012 CFU/ml 농도로 1일 5% 내지 10% 양이다.Camute sprout powder was first added to Saccharomyces sp. The solution was fermented by
각각의 균주 분무량은 표 1과 같다.The spray amount of each strain is shown in Table 1.
상기 발효물에 70%(v/v) 농도로 에탄올을 10배의 중량배수로 첨가하여 2시간 동안 교반 추출하였다. 그 후, 원심분리 및 필터하여 순수한 액상 형태의 추출물을 수득하였다. 수득한 추출물을 회전증발농축기로 분말화하여 발효 카뮤트 새싹 분말을 제조하였다.Ethanol at a concentration of 70% (v/v) was added to the fermented product at a concentration of 10 times by weight, followed by extraction with stirring for 2 hours. Thereafter, centrifugation and filtration were performed to obtain an extract in a pure liquid form. The obtained extract was powdered with a rotary evaporator to prepare fermented kamut sprout powder.
비교예: 발효 전 카뮤트 새싹 분말 제조Comparative Example: Preparation of kamut sprout powder before fermentation
발효 전 카뮤트 새싹 분말을 제조하기 위하여 카뮤트 새싹 건조물에 그의 10배 중량의 해당하는 양의 70% (w/v) 농도의 에탄올을 첨가하여 2시간 동안 교반 추출한다. 그 후, 원심분리 및 필터하여 순수한 액상 형태의 추출물을 수득한다. 수득한 추출물을 회전증발농축기로 분말화하여 발효 전 카뮤트 새싹 분말을 제조하였다. In order to prepare kamut sprout powder before fermentation, 70% (w/v) concentration of ethanol in a corresponding amount of 10 times its weight is added to the dried kamut sprouts, followed by extraction with stirring for 2 hours. Thereafter, centrifugation and filtration are performed to obtain an extract in a pure liquid form. The obtained extract was pulverized with a rotary evaporator to prepare kamut sprout powder before fermentation.
실험예 1: 세포독성 확인Experimental Example 1: Confirmation of cytotoxicity
본 발명에 따른 조성물의 세포독성을 확인하기 위하여 Raw264.7 세포를 사용하였다. Raw264.7 세포에 비교예에 따른 발효하지 않은 카뮤트 새싹 분말(Kamut 발효 전) 및 실시예에 따른 발효 카뮤트 새싹 분말(Kamut 발효 후)을 농도 별로 처리하여 WST-1 assay로 세포의 생존율을 확인하였다. WST는 살아있는 세포의 양을 측정하는 제품으로써 cell viability를 측정하는데 사용된다. 96 well plate에 각각의 셀을 2 X 104 cell/well로 시딩(seeding)하고, 5% CO2, 37℃로 배양기에서 24시간 배양하였다. 배양 후, 세럼 프리 배지로 교체하여 starvation 시키고 24시간 배양하였다. 발효하지 않은 카뮤트 새싹 분말(Kamut 발효 전)과 발효 카뮤트 새싹 분말(Kamut 발효 후)를 각각의 농도로 처리하여 5% CO2, 37℃로 배양기에서 24시간 배양한 후, WST-1 solution을 처리하고 2시간 배양하였다. 배양 후, 흡광도 측정 기기(Multiskan GO, Thermo Fisher Scientific)로 450 nm에서 흡광도를 측정하였다. 결과를 바탕으로 아래의 세포생존율을 산출식을 통해 세포의 생존율을 시료를 처리하지 않은 군과 비교하여 %로 나타내었다.Raw264.7 cells were used to confirm the cytotoxicity of the composition according to the present invention. Raw264.7 cells were treated with unfermented kamut sprout powder (before Kamut fermentation) according to the comparative example and fermented kamut sprout powder (after Kamut fermentation) according to the embodiment by concentration, and cell viability was evaluated by WST-1 assay. Confirmed. WST is a product that measures the amount of living cells and is used to measure cell viability. Each cell was seeded at 2
세포 생존율(%) = [시료첨가군의 흡광도 (450 nm) / 시료 무첨가군의 흡광도 (450 nm)] × 100Cell viability (%) = [Absorbance of the sample added group (450 nm) / Absorbance of the non-sample group (450 nm)] × 100
도 1에 도시된 세포 독성 결과와 같이, 발효 전 Kamut는 약 0.6mg/ml 농도 이하에서 독성이 없음을 확인하였고, 발효 후 Kamut는 1.25mg/ml 농도 이하에서 독성이 없음을 확인하였다. 즉, 발효 후 Kamut(실시예)는 발효 전 Kamut에 비하여 2배 이상 높은 농도로 사용할 수 있음을 알 수 있다.As shown in the cytotoxicity results shown in FIG. 1 , it was confirmed that Kamut had no toxicity at a concentration of about 0.6 mg/ml or less before fermentation, and it was confirmed that Kamut had no toxicity at a concentration of 1.25 mg/ml or less after fermentation. That is, it can be seen that Kamut (Example) after fermentation can be used at a concentration twice or more higher than that of Kamut before fermentation.
실험예 2: 유효성분 함량 분석Experimental Example 2: Active ingredient content analysis
발효 전, 후 카뮤트 새싹 분말의 유효성분 함량 변화를 확인하였다. 구체적으로 0.1% w/v Gallic acid 표준 용액을 제조하여 10 내지 100 ppm 농도로 희석 후 2 N Folin & Ciocalteu's phenol reagent를 넣어 상온에서 5분간 반응시킨다. 이 후 7% w/v Na2CO3 용액과 증류수를 첨가하여 상온에서 90분간 반응시킨다. 반응 종료 후 750nm 파장에서 흡광도를 측정하여 검정곡선을 구하고, 카뮤트 새싹 분말의 발효 전, 후 분말 시료도 상기와 같은 방법으로 반응시킨 후 750nm 파장에서 흡광도를 측정하여 Gallic acid 검정곡선에 대입하여 폴리페놀(polyphenol) 함량을 분석하였다. 이 때 대조군으로 증류수를 이용하였다.Changes in the active ingredient content of the kamut sprout powder before and after fermentation were confirmed. Specifically, a 0.1% w/v gallic acid standard solution is prepared, diluted to a concentration of 10 to 100 ppm, 2 N Folin &Ciocalteu's phenol reagent is added, and reacted at room temperature for 5 minutes. After that, 7% w/v Na 2 CO 3 solution and distilled water are added and reacted at room temperature for 90 minutes. After completion of the reaction, the absorbance is measured at 750 nm wavelength to obtain a calibration curve, and the powder samples before and after fermentation of kamut sprout powder are also reacted in the same way as above. The phenol (polyphenol) content was analyzed. In this case, distilled water was used as a control.
도 2 및 표 2에 도시된 바와 같이, 발효 전의 카뮤트 새싹 분말은 폴리페놀의 함량이 2.52%로 배양 시간에 따른 변화가 없다. 반면, 발효한 카뮤트 새싹 분말(실시예)는 배양 시간에 따라 폴리페놀의 함량이 증가하는 것을 확인하였다.As shown in FIG. 2 and Table 2, the kamut sprout powder before fermentation had a polyphenol content of 2.52%, and there was no change with the incubation time. On the other hand, in the fermented kamut sprout powder (Example), it was confirmed that the polyphenol content increased with the incubation time.
실험예 3: 항산화 효과 확인Experimental Example 3: Confirmation of antioxidant effect
발효 전, 후 카뮤트 새싹 분말의 항산화 효과를 확인하기 위하여 DPPH(2,2-diphenyl-1-picryl hydrezyl) 라디칼 소거능 분석을 실시하였다. 1mM DPPH 용액을 메탄올에 녹여 즉시 사용하였다. 시료 용액과 DPPH 용액을 1:1로 혼합하여, 상온 암소에서 30 분간 방치하였다. 이때 양성 대조군(positive control)으로 아스코르브산(ascorbic acid) 1 mg/ml을 사용하였다. 30분 방치 후, 흡광도 측정 기기 (Multikan Go, Thermo Fisher Scientific)를 이용하여 540nm에서 흡광도를 측정하였다. 대조군(control) 대비 라디컬 비율 (%)를 산출하였다.DPPH (2,2-diphenyl-1-picryl hydrezyl) radical scavenging activity analysis was performed to confirm the antioxidant effect of kamut sprout powder before and after fermentation. A 1 mM DPPH solution was dissolved in methanol and used immediately. The sample solution and the DPPH solution were mixed at a ratio of 1:1, and allowed to stand in the dark at room temperature for 30 minutes. At this time, 1 mg/ml of ascorbic acid was used as a positive control. After standing for 30 minutes, absorbance was measured at 540 nm using an absorbance measuring instrument (Multikan Go, Thermo Fisher Scientific). The radical ratio (%) compared to the control group was calculated.
도 3에 도시된 바와 같이, 발효하지 않은 카뮤트 새싹 분말(Kamut 발효 전)은 5mg/ml 농도에서 약 58%의 자유라디칼 소거능을 나타내었다. 그에 비하여, 발효한 카뮤트 새싹 분말(Kamut 발효 후)은 5mg/ml 동일 농도에서 약 72%로 더 높은 자유라디칼 소거능을 나타내었다.As shown in FIG. 3 , unfermented kamut sprout powder (before Kamut fermentation) exhibited a free radical scavenging activity of about 58% at a concentration of 5 mg/ml. In contrast, the fermented kamut sprout powder (after Kamut fermentation) exhibited a higher free radical scavenging ability by about 72% at the same concentration of 5 mg/ml.
실험예 4: 항염 효과 확인Experimental Example 4: Confirmation of anti-inflammatory effect
실험예 4-1: COX-2 mRNAExperimental Example 4-1: COX-2 mRNA
발효 전, 후 카뮤트 새싹 분말의 항염 효과를 확인하기 위하여 COX-2 mRNA 발현 정도를 분석하였다. HaCaT cell line을 10% FBS(fetal bovine serum)와 1X 페니실린/스트렙토마이신(P/S)이 함유된 DMEM 배지에 배양하고 세포를 카운팅한 뒤 6-well 플레이트에 5 x 105 cell/well이 되도록 희석하여 시딩하고 5% CO2, 37℃로 배양기에서 24시간 배양하였다. 배양 후 세럼 프리 배지로 교체 후 starvation 시키고, 카뮤트 발효 전 후 시료를 농도별로 희석하여 처리하였다. 양성 대조군(positive control)으로는 덱사메타손(dexamethasone, Dex)을 사용하였다. 10㎍/ml으로 처리 후 시료와 함께 5% CO2, 37℃로 배양기에서 1시간 배양하고 LPS가 1㎍/ml이 되도록 처리 후 5% CO2, 37℃로 배양기에서 24시간 배양하였다. 이후 배지를 제거하고 DPBS로 워싱 하였다. 그 후 각 시료의 세포에서 RNA Extraction Kit (TaKaRa Mini BEST Universal kit)를 이용해서 RNA를 분리한 뒤 Qubit Fluorometer로 RNA를 정량한 후, 각각 1㎍의 RNA를 사용하여 증폭기에서 cDNA를 합성하였다(Step One Plus, Applied Biosystems). 합성된 cDNA에 타겟 단백질인 COX-2 프라이머와 시아닌 염료인 사이버그린(Power SYBR Green PCR Master Mix, Applied Biosystems)을 첨가한 혼합물을 이용하여 Real-Time PCR 기계에서 실시간 중합효소 연쇄반응을 실시함으로써 최종적으로 COX-2 유전자의 발현 정도를 확인하였다. 유전자의 발현량은 GAPDH 유전자에 대한 보정을 통해 최종적으로 분석하고, 도출된 결과를 도 4에 나타내었다. 유전자의 발현을 확인하기 위해 사용한 프라이머를 표 1에 나타내었다. COX-2 mRNA expression level was analyzed to confirm the anti-inflammatory effect of kamut sprout powder before and after fermentation. HaCaT cell line was cultured in DMEM medium containing 10% fetal bovine serum (FBS) and 1X penicillin/streptomycin (P/S), counted, and 5 x 10 5 cells/well in a 6-well plate. It was seeded by dilution and incubated for 24 hours in an incubator at 5% CO 2 , 37°C. After incubation, the medium was replaced with a serum-free medium, followed by starvation, and the samples before and after Kamute fermentation were diluted and processed by concentration. As a positive control, dexamethasone (Dex) was used. After treatment with 10㎍/ml, the sample was incubated with the sample at 5% CO 2 , 37° C. for 1 hour, and LPS was treated to 1 μg/ml, followed by 5% CO 2 , incubated at 37° C. for 24 hours in an incubator. Then, the medium was removed and washed with DPBS. After that, RNA was isolated from the cells of each sample using the RNA Extraction Kit (TaKaRa Mini BEST Universal kit), and RNA was quantified with a Qubit Fluorometer, and cDNA was synthesized in an amplifier using 1 μg of each RNA (Step One Plus, Applied Biosystems). Finally, by performing a real-time polymerase chain reaction in a real-time PCR machine using a mixture in which the target protein, COX-2 primer, and the cyanine dye, Power SYBR Green PCR Master Mix, Applied Biosystems are added to the synthesized cDNA. to confirm the expression level of the COX-2 gene. The expression level of the gene was finally analyzed through correction for the GAPDH gene, and the derived results are shown in FIG. 4 . The primers used to confirm the expression of the gene are shown in Table 1.
도 4에 도시된 바와 같이, LPS 단독 처리군에 대하여, 발효하지 않은 카뮤트 새싹 분말(Kamut 발효 전)은 600ug/ml 농도에서 약 65%로 COX-2 유전자 발현을 억제하였다. 그에 비하여, 발효한 카뮤트 새싹 분말(Kamut 발효 후)은 동일한 농도에서 약 80%로 COX-2 유전자 발현을 억제하였다.As shown in FIG. 4 , for the LPS-only treatment group, unfermented kamut sprout powder (before Kamut fermentation) inhibited COX-2 gene expression by about 65% at a concentration of 600 ug/ml. In contrast, fermented kamut sprout powder (after Kamut fermentation) inhibited COX-2 gene expression by about 80% at the same concentration.
실험예 4-2: IL-6 mRNAExperimental Example 4-2: IL-6 mRNA
발효 전, 후 카뮤트 새싹 분말의 항염 효과를 확인하기 위하여 IL-6 mRNA 발현 정도를 분석하였다. 실험예 4-1에 기재한 것과 동일한 세포를 사용하여 동일 조건으로 실험을 진행하였다. 최종적으로 실시간 중합효소 연쇄반응을 진행하는 과정에서 COX-2 프라이머 대신 IL-6 프라이머를 사용하였다. 유전자의 발현량은 GAPDH 유전자에 대한 보정을 통해 최종적으로 분석하였고 도출된 결과는 도 5에 나타내었다. 유전자 발현을 확인하기 위하여 사용한 프라이머를 표 2에 나타내었다.IL-6 mRNA expression level was analyzed to confirm the anti-inflammatory effect of kamut sprout powder before and after fermentation. Experiments were conducted under the same conditions using the same cells as those described in Experimental Example 4-1. Finally, during the real-time polymerase chain reaction, the IL-6 primer was used instead of the COX-2 primer. The expression level of the gene was finally analyzed through correction for the GAPDH gene, and the derived results are shown in FIG. 5 . The primers used to confirm gene expression are shown in Table 2.
도 5에 도시된 바와 같이, 리포폴리사카라이드(LPS) 단독 처리군에 대하여, 발효하지 않은 카뮤트 새싹 분말(Kamut 발효 전)은 60ug/ml 농도에서 약 4%로 IL-6 유전자 발현을 억제하였다. 그에 비하여, 발효한 카뮤트 새싹 분말(Kamut 발효 후)은 동일한 농도에서 약 35%로 IL-6 유전자 발현을 억제하였다.As shown in FIG. 5 , for the lipopolysaccharide (LPS) alone treatment group, unfermented kamut sprout powder (before Kamut fermentation) inhibited IL-6 gene expression by about 4% at a concentration of 60 ug/ml. did In contrast, fermented kamut sprout powder (after Kamut fermentation) inhibited IL-6 gene expression by about 35% at the same concentration.
상기한 바와 같이, 본 발명에 따른 조성물은 세포에 대한 독성을 감소하고, 항염 및 항산화 효과가 있음을 확인하였다.As described above, it was confirmed that the composition according to the present invention reduces toxicity to cells, and has anti-inflammatory and antioxidant effects.
이상 본 발명의 실시예들을 설명하였으나, 본 발명은 상기 실시예들에 한정되는 것이 아니라 서로 다른 다양한 형태로 제조될 수 있으며, 본 발명이 속하는 기술 분야에서 통상의 지식을 가진 자는 본 발명의 기술적 사상이나 필수적인 특징을 변경하지 않고서 다른 구체적인 형태로 실시될 수 있다는 것을 이해할 수 있을 것이다. 그러므로 이상에서 기술한 실시예들은 모든 면에서 예시적인 것이며, 한정적이 아닌 것으로 이해해야만 한다.Although the embodiments of the present invention have been described above, the present invention is not limited to the above embodiments, but may be manufactured in a variety of different forms, and those of ordinary skill in the art to which the present invention pertains will appreciate the technical spirit of the present invention. However, it will be understood that the invention may be embodied in other specific forms without changing essential features. Therefore, it should be understood that the embodiments described above are illustrative in all respects and not restrictive.
Claims (14)
상기 발효물을 용매 추출하여 추출물을 수득하는 단계를 포함하고,
상기 발아식물이 카뮤트 새싹을 포함하고,
상기 발효물을 제조하는 단계가 Saccharomyces sp.를 접종 및 발효한 다음, Lactobacillus sp.를 접종하여 발효하는 단계를 순차적으로 포함하는, 발아식물 발효 추출물을 포함하는 조성물의 제조방법.preparing a fermented product by inoculating a germinating plant with microorganisms; and
solvent extraction of the fermented product to obtain an extract,
The germinating plant comprises a kamut sprout,
After the step of preparing the fermented product is inoculated and fermented with Saccharomyces sp., and then inoculated with Lactobacillus sp.
상기 발아식물이 새싹 귀리, 밀크시슬 새싹, 알팔파 새싹 및 브로콜리 새싹으로 이루어지는 군으로부터 선택되는 하나 이상을 더 포함하는 것인, 발아식물 발효 추출물을 포함하는 조성물의 제조방법.According to claim 1,
The method for producing a composition comprising a germinated plant ferment extract, wherein the germinated plant further comprises one or more selected from the group consisting of sprout oats, milk thistle sprouts, alfalfa sprouts, and broccoli sprouts.
상기 발효물을 제조하는 단계가 상기 발아식물에 사카로미세스 세레비시아 또는 락토바실러스 사케이를 1일 1 내지 5회 접종하여 4 내지 10일 동안 발효하는 단계를 포함하는 것인, 발아식물 발효 추출물을 포함하는 조성물의 제조방법.According to claim 1,
In which the step of preparing the fermented product comprises the step of inoculating the germinated plant with Saccharomyces cerevisiae or Lactobacillus saccharis 1 to 5 times a day and fermenting for 4 to 10 days, a fermented extract of a germinated plant A method for preparing a composition comprising
상기 발효물을 제조하는 단계가 상기 발아식물에 Saccharomyces sp.를 1일 1 내지 5회 접종하여 2 내지 5일 동안 발효하는 단계; 및
Lactobacillus sp.를 1일 1 내지 5회 접종하여 2 내지 5일 동안 발효하는 단계를 순차적으로 포함하는 것인, 발아식물 발효 추출물을 포함하는 조성물의 제조방법.According to claim 1,
The step of preparing the fermented product is Saccharomyces sp. Inoculating 1 to 5 times a day and fermenting for 2 to 5 days; and
Lactobacillus sp. A method for producing a composition comprising a fermented germinated plant extract, which sequentially comprises the step of inoculating 1 to 5 times a day and fermenting for 2 to 5 days.
상기 발효물을 제조하는 단계가 상기 발아식물에 Saccharomyces sp. 및 Lactobacillus sp.를 2:1 내지 10:1 중량비로 접종하는 단계를 포함하는 것인, 발아식물 발효 추출물을 포함하는 조성물의 제조방법.According to claim 1,
The step of preparing the fermented product is Saccharomyces sp. and Lactobacillus sp. A method for producing a composition comprising a germinated plant ferment extract comprising the step of inoculating a 2:1 to 10:1 weight ratio.
상기 용매가 물, 에탄올 또는 이들의 조합을 포함하는 것인, 발아식물 발효 추출물을 포함하는 조성물의 제조방법.According to claim 1,
The method for producing a composition comprising a germinated plant ferment extract, wherein the solvent comprises water, ethanol, or a combination thereof.
상기 추출물을 수득하는 단계가 상기 발아식물에 5 내지 20중량 배수의 용매를 첨가하고, 10초 내지 12시간 동안 교반하는 단계를 포함하는 것인, 발아식물 발효 추출물을 포함하는 조성물의 제조방법.According to claim 1,
A method for producing a composition comprising a fermented extract of a germinated plant, wherein the step of obtaining the extract comprises adding a solvent of 5 to 20 weight multiples to the germinated plant, and stirring for 10 seconds to 12 hours.
상기 추출물을 농축하여 분말을 제조하는 단계를 더 포함하는 것인, 발아식물 발효 추출물을 포함하는 조성물의 제조방법.According to claim 1,
Concentrating the extract to further comprise the step of preparing a powder, the method for producing a composition comprising a germinated plant ferment extract.
상기 조성물이 항염 또는 항산화용인 것인, 발아식물 발효 추출물을 포함하는 화장료 조성물.11. The method of claim 10,
A cosmetic composition comprising a germinated plant ferment extract, wherein the composition is for anti-inflammatory or antioxidant.
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