KR100830403B1 - Natural cosmetic composition having the cactus fermented liquid and grape seed extracts - Google Patents

Abstract

The present invention relates to a natural cosmetic composition containing palm cactus fermentation broth and grape seed extract.

Natural cosmetic composition of the present invention is a palm cactus fermentation broth containing 10 to 80% by weight relative to the total weight of the cosmetic composition, grape seed extract contains 0.1 to 10% by weight relative to the total weight of the cosmetic composition, prepared by adding other materials according to the cosmetic formulation It consists of doing.

The present invention provides a natural cosmetic composition containing the palm cactus fermentation broth and grape seed extract, and provides a natural cosmetic composition excellent in skin moisturizing, skin elasticity and anti-wrinkle effect.

Cosmetics, cactus cactus, fermentation, grape seed, skin moisture, elasticity, wrinkle suppression

Description

NATURAL COSMETIC COMPOSITION HAVING THE CACTUS FERMENTED LIQUID AND GRAPE SEED EXTRACTS}

The present invention relates to a natural cosmetic composition containing palm cactus fermentation broth and grape seed extract.

Recently, cosmetics applied to the face of a person is also known to be a natural material that humans can eat, have no side effects on the skin and excellent efficacy, making various attempts to manufacture a natural cosmetic composition using a variety of natural materials.

Among them, Opuntia ficus indica is a perennial plant that grows in its natural state in the open field in four seasons, and is a viable plant that survives cold below 20 degrees Celsius.

It grows on soil mixed with loess and sandy soil, and does not die from pests, so it is not necessary to use herbicides or chemical fertilizers such as pesticides, and it is a potent plant, especially palm cactus, which contains antioxidants such as phenolic flavonoids. It is known to contain an amount of anti-aging, as well as anti-cancer, strengthening immunity against various adult diseases.

In addition, this palm cactus contains dietary fiber, vitamin C, calcium, minerals and amino acids, complex polysaccharides, and other various nutrients important to the human body, and is widely used as a health food.

Recently, there have been attempts to use cosmetics or skin external preparations using cactus, which is known for such efficacy.

Korean Patent Application Publication No. 10-0435855 (External skin composition containing palm cactus extract) is a skin external composition comprising palm cactus extract extracted with water or organic solvent as a main component. Disclosed is a composition for external application for skin that is effective in enhancing water content, preventing skin drying, preventing and improving atopic and contact dermatitis symptoms, and enhancing skin barrier function.

In addition, Korean Laid-Open Patent Publication No. 10-2005-0093322 (skin anti-aging cosmetics), extracts the cheonnyeoncho cactus with at least one solvent selected from the group consisting of water, lower alcohol, ethyl acetate and acetone, and extracts the cheonnyeoncho cactus, It is disclosed that the extract contains 0.1 to 10.0% by weight relative to the total weight of the cosmetic for preventing skin aging cosmetics.

However, conventionally, as described above, a cactus is extracted with water or an organic solvent and is a natural cosmetic composition using the extract, and when heated at a high temperature, the useful components of the cactus are destroyed, so there is a problem in that it does not exert its efficacy properly. When heated, there is a problem that it is difficult to extract useful components of the cactus.

To solve the problem of the extract as described above is a cactus fermented fermented cactus.

In Korean Laid-Open Patent Publication No. 2003-0094879 (Palm cactus fermentation product and its preparation method), after crushing palm cactus fruit, the fermentation accelerator and the useful microbial agent are mixed, and the fermentation broth obtained by fermentation for 2 weeks or more at room temperature is lyophilized. Disclosed is a fermented cactus fermentation product, which is composed of a powder.

However, when the fermented cactus fermented using the microorganisms as described above, there was a problem that the recovery power of the useful component is lowered.

Therefore, it is necessary to study a cactus fermentation broth that contains much more useful ingredients of cactus than the conventional cactus fermentation broth as above, and has excellent efficacy, and develops a natural cosmetic composition containing such a cactus fermentation broth with less side effects, and moisturizes the skin and There is a need for further research on natural cosmetic compositions having excellent antibacterial effects.

On the other hand, grape seed (Grape seed) has recently been rich in polyphenols, showing antioxidant and cholesterol inhibitory effects, and excellent in anti-aging, and containing catechin ingredient, has detoxification, bactericidal effect and anticancer effect, and skin elasticity. It is known to promote and prevent aging, and its use is diversified.

Korean Patent Publication No. 10-0339271 (grape seed extract natural anti-filling agent) is an extract extracted by heating or heating grape seeds using water or an organic solvent, alone or mixed with mugwort, green tea, black tea, lettuce extract, etc. It discloses about the natural anti-cavity composition which suppresses activity and shows antimicrobial activity.

In addition, Korean Patent Publication No. 10-0448447 (composition containing a grape seed extract having alpha glucosidase inhibitory activity, preparation method and use thereof), alpha glucosidase was prepared by adjusting the pH by adding water to grape seed. It is disclosed that the composition is excellent in the effect of inhibiting the action of the agent to inhibit the absorption of glucose in the body to regulate blood glucose.

However, as described above, the conventional grape seed extract is prepared by extracting at high temperature using water or an organic solvent, or by adjusting the pH and extracting using an organic solvent. There was a problem such as extract only a part.

In order to solve the above problems, the present invention is to prepare a fermented cactus fermentation broth and grape seed extract containing a large amount of useful components, to prepare a natural cosmetic composition containing them.

In addition, an object of the present invention is to provide a natural cosmetic composition excellent in skin moisturizing, skin elasticity and anti-wrinkle effect containing the palm cactus fermentation broth and grape seed extract of the present invention.

The present invention relates to a natural cosmetic composition containing palm cactus fermentation broth and grape seed extract.

Natural cosmetic composition of the present invention is a palm cactus fermentation broth containing 10 to 80% by weight relative to the total weight of the cosmetic composition, grape seed extract contains 0.1 to 10% by weight relative to the total weight of the cosmetic composition, prepared by adding other materials according to the cosmetic formulation It consists of doing.

At this time, the other materials added according to the cosmetic formulation uses the additive material of the conventional natural cosmetic composition using natural materials such as vegetable oil.

Palm cactus fermentation broth used in the present invention is to prepare the rice malt juice and wort respectively, to prepare the rice malt juice culture medium to cultivate the white mold, to prepare the wort culture medium to cultivate the white mold, After cultivating the white mold together to prepare a yeast culture solution, this yeast culture solution was repeatedly cultured using rice malt juice, and the culture was expanded, and the expanded yeast culture solution was prepared by anaerobic fermentation using wort to prepare a liquid fermentation agent. Next, the palm cactus is washed and pulverized, and sugar is mixed with the ground powder in a weight ratio of 1: 1, and the mixture and water are mixed in a weight ratio of 1:10 to 1:20, and here Add sugar or oligosaccharide so that the sugar content is 12 to 18 Brix, and the liquid fermentation agent prepared here is 1 to 5 wt% The mixture was then mixed and fermented at 30 to 45 ° C. for 5 to 8 days, and then the fermented product was placed in a stirrer and stirred at 50 to 60 ° C. for 2 to 7 hours to remove alcohol components. To prepare the fermented cactus fermentation.

In addition, the grape seed extract used in the present invention, after washing the grape seed, and mixing the washed grape seed and water in a weight ratio of 1: 1 to 1: 3, put in a water bath and maintain the internal temperature at 50 ~ 60 ℃ 2 Extracted by heating in a hot water for ~ 4 days, the extract is filtered and then put in a cooler and aged for 3 to 7 days at 1 ~ 10 ℃ to prepare a grape seed extract.

Opuntia ficus indica contains more antioxidant flavonoids than any other plant group, so it is known to be effective in preventing aging and strengthening immunity against cancer and various adult diseases. It is a plant that contains calcium, minerals and amino acids, complex polysaccharides, and various nutrients important to the human body.

The components of the palm cactus, the main material of the present invention, are shown in Table 1 below.

Table 1 Constituents of Palm Cactus

ingredient protein carbohydrate Ash Fat amino acid stem 28% 32% 13% 0.77% 0.23% Fruit 21% 35% 6% 3.33% 0.21% Root 20% 21% 7% 2.14% 0.13%

However, it is rich in such useful ingredients, and its physiological activity is also difficult to use directly because it is very viscous and sticky liquid when pulverized in order to use excellent palm cactus. It is weak and there is a problem that useful components are destroyed.

Therefore, by applying the applicant of the present invention to the patent application 10-2005-0029005 (millennial cactus fermentation broth and food using it) that has not yet been published, the palm cactus fermentation broth is adapted to be suitable as a natural cosmetic composition Prepared.

In addition, even when the palm cactus fermentation broth of the present invention using the liquid fermentation agent used in the preparation of the millennial cactus fermentation broth, the applicant of the present invention, the cactus difficult to ferment, while easily fermenting cactus-specific viscosity, so as not to destroy useful components It was.

In other words, the liquid fermentation agent used in the present invention, the rice malt juice and rice malt juice to 1 to 3% by volume of agar to melt the rice malt juice culture medium, and then put the rice malt juice culture medium into the incubator at 20 ~ 35 ℃ 2 In the first step of culturing white mold by incubating for 5 days, add 1 to 3% by volume of agar to the volume of wort and wort to melt wort culture medium, and then add wort culture medium to the incubator. Incubate the white mold by incubating the white mold at 35 ℃ for 2 to 5 days, put the white mold cultivated in the first process and the white mold cultivated in the second process in the incubator, and put the rice malt juice so that the mold is submerged. Next, the third step of incubation for 12 to 24 hours at 20 to 40 ℃, the wort is added to the culture solution 5 to 15 times the weight of the rice malt juice added in the third process, at 20 to 35 ℃ for 12 to 24 hours Anaerobic fermentation process 4 To prepare a liquid fermentation agent.

 Conventional traditional liquor that uses liquor to make liquor is based on 55% alcohol concentration. When making liquor by the traditional method, the liquor production rate can only produce up to 56% of the weight of raw materials, but it is based on 50% alcohol. When the liquor is manufactured using the above-mentioned liquid fermentation agent, the turnout rate of corn reaches 80 to 100%, and the turnout rate reaches 100% when using rice as a raw material. In addition, this liquid fermentation agent is a fermentation agent that can easily ferment raw raw materials when making alcohol.

Using the liquid fermentation agent to prepare a fermented cactus fermentation broth to suit the cosmetic composition.

That is, the palm cactus fermentation broth, which is the main material of the present invention, is crushed after washing the fruit, stem or root of the palm cactus, and the sugar is mixed with the ground product in a weight ratio of 1: 1.

At this time, when the palm cactus is crushed, the viscosity is strong and sticky, and since it is difficult to use it as it is, sugar is added to it to be released gently.

However, when cactus grounds and sugar are simply mixed in a 1: 1 ratio, it is difficult to mix properly, so the cactus grounds and sugars are alternately layered and mixed.

The mixture and water are mixed at a weight ratio of 1: 10 to 1: 20, and sugar or oligosaccharide is added thereto so that the sugar content is 12 to 18 brix, and the liquid fermentation agent prepared there is the weight of palm cactus pulverized as raw material. Add 1 to 5% by weight and mix.

The mixture was fermented at 30 to 45 ° C. for 5 to 8 days, then placed in a stirrer and stirred at 50 to 60 ° C. for 2 to 7 hours to remove alcohol components, and then filtered by stirring the mixture to prepare a fermented cactus fermentation broth. do.

At this time, the stirring time for removing the alcohol component may vary depending on the amount of the mixture, and when the alcohol component is not properly removed due to the amount of the mixture, the above process may be repeated 2 to 3 times.

Palm cactus fermentation broth prepared in this way is added to 10 to 80% by weight of the total weight of the natural cosmetic composition to prepare a natural cosmetic composition of the present invention.

Palm cactus fermentation broth can be adjusted according to the formulation and skin type of the cosmetic composition, less than 10% by weight of the total weight of the composition is less effective.

Palm cactus fermentation broth is excellent in itself, but 10 to 80% by weight of the total weight of the composition is suitable in consideration of the addition ratio and economical efficiency of other cosmetic compositions.

In addition, grape seed (Grape seed), one of the main ingredients of the natural cosmetic composition of the present invention is rich in polyphenols, exhibits antioxidant and cholesterol inhibitory effects, excellent anti-aging, and contains a catechin component, detoxification and bactericidal effect It is known to have anti-cancer effects, enhance skin elasticity and prevent aging.

In the present invention, the grape seed extract having the above-described efficacy is added together with the fermented cactus fermentation broth when preparing the natural cosmetic composition of the present invention to prepare a natural cosmetic composition.

Grape seed extract of the present invention, after washing the grape seed is mixed with the washed grape seed and water in a weight ratio of 1: 1 to 1: 3, and then extracted by heating.

At this time, if the grape seed is directly heated and extracted, the skin of the grape seed is damaged, and the useful components are destroyed. Therefore, the internal temperature is maintained at 50 to 60 ℃ using a water bath. Much is extracted.

After filtering the extract, it is put in a cooler and aged at 1 ~ 10 ℃ for 3 to 7 days to prepare a grape seed extract.

Grape seed extract thus prepared is added to 0.1 to 10% by weight relative to the total weight of the natural cosmetic composition to prepare a natural cosmetic composition of the present invention.

Grape seed extract may vary the addition ratio according to the formulation and skin type of the cosmetic composition, less than 0.1% by weight of the total composition is less effective, considering the effect and the addition ratio of palm cactus 0.1 ~ 10 weight of the total composition It is appropriate to make it%.

Natural cosmetic composition containing the palm cactus fermentation broth and grape seed extract of the present invention has excellent skin moisturizing effect, excellent skin elasticity effect and anti-wrinkle effect.

That is, the palm cactus fermentation broth of the present invention has a much higher moisture content than the palm cactus extract or fermentation broth prepared by the conventional method, and has an excellent skin moisturizing effect.

Grape seed extract of the present invention also has a high water content, it was confirmed that when applied to the cosmetic composition with the palm cactus fermentation broth significantly affects the skin moisturizing effect, was also excellent skin elasticity effect.

In addition, it was found that the cosmetic composition containing the fermented cactus fermentation broth and grape seed extract of the present invention has excellent elastase inhibitory activity and excellent anti-wrinkle function.

In particular, the cosmetic composition of the present invention by using the additives of the natural cosmetic composition with the palm cactus fermentation broth and grape seed extract, to significantly reduce the side effects and further excellent effect.

Hereinafter, the natural cosmetic composition of the present invention will be described in more detail.

<Manufacturing process of natural cosmetic composition containing palm cactus fermentation broth and grape seed extract>

1. Manufacturing Process of Liquid Fermentation Agent

1) First step: cultivation of mold using rice malt juice

After washing 500 g of rice, pour 1.2-1.5 times as much water as the volume of rice and cook.

After the cooked rice is cooled to 40 ° C. or lower, and kept at 20-40 ° C. under aeration for 20 hours, a pale yellow strain appears to produce rice malt.

After washing 1500 g of rice, it is mixed with the prepared rice malt and saccharified by heating at 50 to 75 ° C. for 2 to 4 hours.

After saccharification, the mixture is boiled at 90 to 100 ° C., filtered through a cloth, discarded and the filtered liquid is left until no precipitate is formed.

Next, except for the settled sediment, the top water is prepared separately to prepare rice malt juice.

Put the rice malt juice prepared in a pressure sterilized test tube, and put together 1-3% by volume of agar relative to the volume of rice malt juice to make a rice malt culture medium.

Put the rice malt juice culture medium prepared in the incubator and incubated for 2 to 5 days at 20 ~ 35 ℃ to cultivate white mold on the surface of the medium.

2nd step: cultivation of mold using wort

Prepare dried malt into powder.

Water per 500 g of malt powder is mixed at a rate of 2 to 3 ℃, heated at 40 to 75 ℃ for 2 to 5 hours, saccharified, boiled at 90 to 100 ℃, left at room temperature and cooled to 40 ℃ or less. .

Filter through a sieve, add 2 to 4 egg whites, stir, and filter again to prepare wort.

Put the wort prepared in autoclaved test tube, put together 1 ~ 3% by volume of agar to the volume of wort to make wort culture medium.

Put the wort culture medium prepared in the incubator and incubate for 2 to 5 days at 20 ~ 35 ℃ to culture the white mold on the surface of the medium.

3) Step 3: Cultivation

Take out the white mold cultivated in the first step and the white mold cultivated in the second step into the incubator in the incubator, respectively, and put the rice malt juice prepared in the first half of the first step to the extent that the mold is submerged. Shake and mix evenly and incubate at 20 to 40 ℃ for 12 to 24 hours.

4th stage: fermentation

Put the wort in the culture medium of the third step to 5 to 15 times the weight of the rice malt juice used in the third step, and anaerobic fermentation at 15 to 25 ℃ for 12 to 24 hours to prepare an opaque liquid fermentation agent of the present invention. .

2. Manufacturing Process of Palm Cactus Fermentation Broth

1) First Step: Material Preparation

Buy a cactus on the market and wash it to prepare.

2) second process: grinding

Put the washed cactus into the grinder and grind.

3) Step 3: Add Sugar

In the palm cactus pulverized product of the second step, sugar is added at a weight ratio of 1: 1 and mixed.

At this time, the palm cactus crushed powder and sugar are alternately layered to mix evenly.

4) 4th process: input of the sugar and liquid fermentation agent

Water is mixed into the mixture of the third step in a weight ratio of 1:10 to 1:20, and sugar or oligosaccharide is added thereto and the sugar content is allowed to be 12 to 18 brix.

The liquid fermentation agent prepared here is mixed with 1 ~ 5% by weight relative to the weight of the palm cactus crushed material as a raw material.

5th Process: Fermentation

The mixture of the fourth step is fermented at 30-45 ° C. for 5-8 days.

6) 6th Step: Alcohol Removal

The fermented product of the fifth step is put in a stirrer and the alcohol is removed while stirring at 50 ~ 60 ℃ for 2 to 7 hours.

At this time, the stirring time for removing the alcohol component may vary depending on the amount of the mixture, and when the alcohol component is not properly removed due to the amount of the mixture, the above process may be repeated 2 to 3 times.

7) Step 7: Filtration

The stirring solution from which the alcohol component of the sixth step was removed was filtered to prepare a palm cactus fermentation broth.

3. Manufacturing process of grape seed extract

1) First Step: Material Preparation

Buy grape seeds from the market and wash them to prepare.

2) second process: extraction

Grape seeds and water prepared in the first step is mixed at a weight ratio of 1: 1 to 1: 3, and then put in a water bath and the internal temperature is maintained at 50 ~ 60 ℃ and heated in a water bath for 2 to 4 days to extract the grape seeds.

3) Third Process: Filtration

The extract of the second step is placed in a filter and filtered.

4) 4th process: aging

The filtrate of the third step is put in a cooler and aged at 1 ~ 10 ℃ for 3 to 7 days to prepare a grape seed extract.

4. Preparation of Natural Cosmetic Composition

Palm cactus fermentation broth is 10 to 80% by weight relative to the total weight of the cosmetic composition, grape seed extract is added to 0.1 to 10% by weight relative to the total weight of the cosmetic composition, and is prepared by adding other materials according to the type of cosmetic and skin type.

At this time, the other materials added according to the cosmetic formulation uses the additive material of the conventional natural cosmetic composition using natural materials such as vegetable oil.

Hereinafter, the present invention will be described in detail with reference to Examples and Experimental Examples for palm cactus fermentation broth and grape seed extract containing the present invention, but these do not limit the scope of the present invention.

Example 1 Preparation of Liquid Fermentation Agent

Rice and dried malt were purchased commercially and prepared.

The malt powder was prepared from the dried malt powder.

After washing 500 g of rice, poured 600 g of water, cooked rice, and incubated for 20 hours until light yellow strains appeared at 30 ° C. to make rice malt.

Here, 1500 g of rice was washed, mixed with warmed rice, and heated to 60 ° C for 3 hours to saccharify.

After saccharification, the mixture was boiled to 100 ° C., filtered through a cloth, discarded, and left the filtered liquid for 6 hours.

Then, the rice malt was prepared by putting the upper water apart except the settled sediment.

0.2 ml of agar was added to 10 ml of rice malt juice prepared in a slope test tube and melted at room temperature to make a rice malt juice culture medium.

Rice malt juice culture medium prepared in the incubator was incubated at 25 ℃ for 4 days to incubate white mold.

500 g of the prepared malt powder was mixed with 3 L of water, and then heated to 60 ° C. for 3 hours to saccharify it.

After saccharification, the mixture was heated to 90 ° C., left at room temperature, cooled to 40 ° C. or less, and filtered through a sieve.

Here, only whites were taken out of three eggs, stirred, and filtered again to prepare wort.

0.2 ml of agar was added to 10 ml of the wort prepared in a slope test tube and melted at room temperature to make wort culture medium.

Put the wort culture medium prepared in the incubator was incubated at 25 ℃ for 4 days to incubate white mold.

10 ml of rice malt juice was added to the incubator, and white molds grown in the rice malt culture medium and white molds cultured in the wort culture medium were taken out with a platinum wire and put together in a test tube containing rice malt juice.

The incubator was shaken and evenly mixed, followed by incubation at 35 ° C. for 20 hours.

100 ml of wort was added to the culture solution, an anaerobic fermentation was performed at 20 ° C. for 20 hours to prepare a transparent liquid fermentation agent, and stored at 5 ° C.

<Example 2> Preparation of palm cactus fermentation broth of the present invention

The liquid fermentation agent prepared by the method of Example 1 of the present invention was prepared.

Palm cactus fruit was purchased from the market, washed and prepared.

The washed palm cactus fruits were put in a grinder and ground.

The palm cactus pulverized product 1 kg and sugar 1 kg were mixed, but the palm cactus pulverized product and sugar were alternately layered and mixed to mix evenly.

30 kg of water was added to the mixture, and then 4.8 kg of sugar was added thereto, and the sugar content was adjusted to 15 brix. Then, 30 g of the liquid fermentation agent prepared was added and mixed.

The mixture was fermented at 40 ° C. for 6 days and then placed in a stirrer to remove alcohol by stirring at 50 ° C. for 5 hours.

The stirring solution from which the alcohol component was removed was filtered to prepare a palm cactus fermentation broth of the present invention.

Comparative Example 1 Comparative Preparation of Palm Cactus Extract

Palm cactus fruit was purchased on the market and palm cactus extract was prepared by the conventional method as follows.

That is, after washing the prepared palm cactus, pulverized, put 2 kg of water in 200 g of the palm cactus pulverized, while stirring well, extracted for 2 hours at 80 ℃, and filtered.

4 kg of ethanol was added to the filtrate, and the mixture was left at room temperature for 48 hours, and the precipitate obtained by filtration was dissolved in 2 kg of distilled water, and the filtrate was filtered under reduced pressure.

The concentrate was lyophilized, and then 70% ethanol was added, followed by heating at 80 ° C. for 2 hours to prepare palm cactus extract.

Comparative Example 2 Comparative Preparation of Palm Cactus Fermentation Broth

Palm cactus fruit was purchased on the market and the palm cactus fermentation broth was prepared by the conventional method as follows.

That is, 100 g of molasses and 200 g of a commercially available useful microbial agent (Korea EMRO) were added to 700 g of the prepared Cactus Crushed Powder, and then fermented at room temperature for 2 weeks to prepare a fermentation broth.

Experimental Example 1 Measurement of Skin Moisturizing Effect of Palm Cactus Fermentation Solution of the Present Invention

Palm cactus fermentation broth prepared by the method of Example 2 of the present invention was prepared.

Palm cactus extract of Comparative Example 1 and Palm cactus fermentation broth of Comparative Example 2 prepared by a conventional method were prepared.

Forty women in their 30s and 60s with dry skin were divided into four groups with 10 age groups.

After washing several times with water, the water was removed, and after 5 minutes, purified water, fermentation broth of Example 2, Comparative Example 1 and Comparative Example 2 were corrected.

After 10 minutes, it was measured using a Corneometer (Courage-Khazaka Electronics, Germany; CM 825), and five more times at 10 minute intervals.

In the experiment, the room temperature was 23 ℃, the relative humidity was 22%, and the water content was calculated by the following equation.

The average value was used by measuring three times repeatedly, and the average value for 40 people is described in Table 2 below.

Moisture content (%) = value after sample application-value before sample application

<Table 2> Comparative results of skin moisturizing effect

Minutes Purified water(%) Example 2 (%) Comparative Example 1 (%) Comparative Example 2 (%) 10 8.5 45.2 24.1 29.3 20 5.4 39.1 20.7 24.0 30 0.1 30.8 12.4 15.9 40 0.0 23.7 6.8 8.3 50 0.0 15.3 2.9 5.6 60 0.0 7.6 1.2 2.8

As shown in Table 2, the palm cactus fermentation broth of the present invention, unlike the palm cactus extract or fermentation broth prepared by the conventional method, was found to have a significantly higher water content and a superior sustaining effect.

Example 3 Preparation of Grape Seed Extract of the Present Invention

Commercially prepared grape seeds were washed and washed.

500 g of the prepared grape seed and 1 kg of water were mixed, and then, the mixture was put in a water bath and the internal temperature was maintained at 50 ° C., followed by heating in a water bath for 3 days to extract grape seeds.

The extract was put into a filter and filtered, and the filtrate was put into a cooler and aged at 4 ° C. for 4 days to prepare a grape seed extract.

Example 4 Preparation of Skin Containing Palm Cactus Fermentation Solution and Grape Seed Extract of the Present Invention

Palm cactus fermentation broth prepared by the method of Example 2 of the present invention and grape seed extract prepared by the method of Example 3 were prepared.

The ingredients shown in Table 3 below were added and mixed in the same amounts as described to prepare the skin for oily skin of the present invention.

<Table 3> Preparation Example of Skin for Oily Skin

material 100 ml standard Purified water 86 ml Palm cactus fermentation broth 10 ml Grape Seed Extract 0.4 ml Vitamin E 0.5 ml glycerin 2 ml Hyaluronic acid 1 ml Lavender Essential Oil 0.1 ml

<Example 5> lotion containing the palm cactus fermentation broth and grape seed extract of the present invention

Palm cactus fermentation broth prepared by the method of Example 2 of the present invention and grape seed extract prepared by the method of Example 3 were prepared.

Ewax was first put in a container to melt, and then oil base, jojoba, top, and olive oil were added as shown in Table 4 and heated to 50 ° C.

Palm cactus fermentation broth and grape seed extract were also heated to 50 ° C in another vessel.

The materials of both vessels were placed in a stirrer and stirred at low speed for 10 minutes to thicken.

After the stirring was lukewarm, all the other ingredients of Table 4 were added and mixed, and the mixture was stirred to increase the speed of the stirrer to a cream state, thereby preparing the sensitive skin lotion of the present invention.

Table 4 Preparation Example of Sensitive Skin Lotion

material 100 ml standard Palm cactus fermentation broth 67.75 ml Grape Seed Extract 10 ml Jojoba 5 ml Dot 5 ml olive 5 ml Ewax 3 ml Vitamin E 1 ml glycerin 2 ml Hyaluronic acid 1 ml Grapefruit Seed Extract 0.15 ml Jasmine Essential Oil 0.1 ml

<Example 6> nutrition cream containing palm cactus fermentation broth and grape seed extract of the present invention

Palm cactus fermentation broth prepared by the method of Example 2 of the present invention and grape seed extract prepared by the method of Example 3 were prepared.

Wax was first added to the container to dissolve it, and then oil-based avocado, sweet almond, and rosehip oil were added as shown in Table 5 and heated to 50 ° C.

Palm cactus fermentation broth and grape seed extract were placed in another container and heated to 50 ° C.

The materials from both vessels were mixed and placed in a stirrer to agitate at low speed for 15 minutes to thicken.

After the stirring was lukewarm, all the other ingredients added in Table 5 were added and mixed, and the mixture was stirred to increase the speed of the stirrer to a cream state to prepare a nourishing cream for dry skin (combination of eye cream) of the present invention.

<Table 5> Preparation Example of Nutritional Cream for Dry Skin (Eye Cream Combination)

material 60 g standard Palm cactus fermentation broth 21 g Grape Seed Extract 6 g avocado 4 g Dot 4 g Sweet Almond 4 g Rose Hip 4 g Ewax 5 g Vitamin E 1 g glycerin 2 g Grapefruit Seed Extract 0.5 g Hyaluronic acid 0.8 g Marine Elastin 0.8 g Collagen 0.8 g Ceramide 0.8 g Placenta 0.8 g Aloe veragel 4.4 g Rose Essential Oil 0.1 g

Example 7 Preparation of Essence Serum Containing Palm Cactus Fermentation Solution and Grape Seed Extract of the Present Invention

Palm cactus fermentation broth prepared by the method of Example 2 of the present invention and grape seed extract prepared by the method of Example 3 were prepared.

First, the emulsion wax was dissolved in a container, and oil-based rose hips, borage, evening priming rose, avocado, jojoba, black sesame, sweet almonds, and the like were heated to 50 ° C.

Palm cactus fermentation broth and grape seed extract were placed in another container and heated to 50 ° C.

The two vessels were mixed and placed in a stirrer and stirred at low speed for 10 minutes to thicken.

After the stirring was lukewarm, all the other ingredients of Table 6 were added and mixed, and the mixture was stirred to increase the speed of the stirrer to a cream state to prepare an essence serum for dry skin of the present invention.

<Table 6> Preparation Example of Essence Serum for Dry Skin

material 60 g standard Palm cactus fermentation broth 21 g Grape Seed Extract 0.6 g Rose Hip 3 g Borage 3 g Evening Priming Rose 3 g avocado 3 g Jojoba 5 g Black Sesame 2 g Sweet Almond 3 g Emulsifying Power Wax 1 g Vitamin E 1 g glycerin 2 g Grapefruit Seed Extract 1.5 g Hyaluronic acid 1 g Marine Elastin 1 g Collagen 1 g Ceramide 1 g Placenta 1 g lecithin 1 g Aloe veragel 4.8 g Neroli essential oil 0.1 g

Example 8 Preparation of Cleansing Cream Containing Palm Cactus Fermentation Solution and Grape Seed Extract of the Present Invention

Palm cactus fermentation broth prepared by the method of Example 2 of the present invention and grape seed extract prepared by the method of Example 3 were prepared.

The beeswax and emulsion wax were first dissolved in a container, and the oil base was added to jojoba, precut, and olive, and heated to 50 ° C.

Palm cactus fermentation broth and grape seed extract were placed in another container and heated to 50 ° C.

The two vessels were mixed and placed in a stirrer and stirred at low speed for 15 minutes to thicken.

After the stirring was lukewarm, all the essential oils were added, and the stirring speed was increased to a cream state to prepare a cleaning cream of the present invention.

<Table 7> Preparation Example of Cleansing Cream

material 100 g standard Palm cactus fermentation broth 53.8 g Grape Seed Extract 10 g Jojoba 10 g Epricut 10 g olive 10 g Emulsifying Wax 3 g Beads wax 3 g Peppermint Essential Oil 0.1 g Geranium Essential Oil 0.1 g

<Example 9> Preparation of sunscreen containing palm cactus fermentation broth and grape seed extract of the present invention

Palm cactus fermentation broth prepared by the method of Example 2 of the present invention and grape seed extract prepared by the method of Example 3 were prepared.

The wax was first dissolved in a container, and the oil base was added to jojoba and black sesame, which were heated to 50 ° C.

Palm cactus fermentation broth and grape seed extract were added to another vessel and heated to 50 ° C.

The two vessels were mixed and placed in a stirrer and stirred at low speed for 15 minutes to thicken.

After the stirring was lukewarm, all the other additives of Table 8 were added and mixed, and the stirring speed was increased to a cream state to prepare a sunscreen agent for oily skin (SPF25) of the present invention.

<Table 8> Preparation Example of Sunscreen Agent for Oily Skin

material 100 g standard Palm cactus fermentation broth 60 g Grape Seed Extract 5 g Jojoba 7 g Black Sesame 8 g Ewax 4.5 g Vitamin E 1 g glycerin 2 g Grapefruit Seed Extract 1.5 g Hyaluronic acid 1 g Titanium dioxide 2.4 g Zinc oxide 2.4 g Aloe veragel 5 g Geranium Essential Oil 0.1 g Lavender Essential Oil 0.1 g

Experimental Example 2 Experiment for Measuring Skin Moisturizing Effect of Cream of the Present Invention

A nourishing cream for dry skin prepared in the same manner as in Example 6 of the present invention was prepared.

To prepare a cream in the same manner as in Example 6, the nutrition cream of Comparative Examples 3 to 5 prepared by varying the amount of the palm cactus fermentation broth and grape seed extract as shown in Table 9.

<Table 9> Comparative Preparation of Nutritional Cream

division Palm cactus fermentation broth Grape Seed Extract (%) Purified water(%) Example 6 21 g (35%) 6 g (10%) 0 Comparative Example 3 10.2 g (17%) 6 g (10%) 10.2 (17%) Comparative Example 4 0 6 g (10%) 21 g (35%) Comparative Example 5 21 g (35%) 3 g (5%) 3 g (5%) Comparative Example 6 21 g (35%) 0 6 g (10%) Comparative Example 7 0 0 27 g (45%)

The nutrition moisturizing cream prepared as shown in Table 9 above was measured and compared with the skin moisturizing effect as follows.

That is, six women in their 20s and 60s with dry skin were divided into six groups with equal age groups.

After washing several times with water, the water was removed, and after 5 minutes, the creams of Example 6 and Comparative Examples 3 to 7 were applied properly.

After 10 minutes, it was measured using a Corneometer (Courage-Khazaka Electronics, CM 825), and five more times at 10 minute intervals.

In the experiment, the room temperature was 23 ℃, the relative humidity was 22%, and the water content was calculated by the following equation.

The average value was used by measuring three times repeatedly, and the average value for 40 people is described in Table 2 below.

Moisture content (%) = value after sample application-value before sample application

<Table 10> Comparative results of skin moisturizing effect on the nourishing cream of the present invention

division 10 minutes 20 minutes 30 minutes 40 mins 50 minutes 60 mins Example 6 56.5% 51.4% 45.8% 39.2% 34.6% 29.1% Comparative Example 3 48.6% 42.7% 36.9% 29.3% 23.6% 17.9% Comparative Example 4 38.7% 33.1% 27.7% 22.1% 16.8% 11.7% Comparative Example 5 53.1% 48.5% 43.6 5 38.0% 32.5% 27.6% Comparative Example 6 50.2% 46.0% 41.3% 36.7% 31.1% 24.3% Comparative Example 7 20.1% 15.8% 8.9% 3.5% 1.7% 0.5%

As shown in Table 10, it was found that the water content greatly depends on the amount of the palm cactus fermentation broth and grape seed extract of the present invention.

This can be seen that the grape seed extract, along with the fermented cactus fermentation broth of the present invention, also significantly affects skin moisturization.

Experimental Example 3 Anti-wrinkle Effect Experiment on Nutritional Cream of the Present Invention

A dry skin eye cream combined nutrition cream prepared in the same manner as in Example 6 of the present invention was prepared.

In addition, comparative compositions of Comparative Examples 3 to 7 prepared in Experimental Example 2 were prepared.

The elastase inhibitory activity was measured with respect to the cosmetic composition of Example 6 and Comparative Examples 3-7 which were prepared.

Elastase inhibitory activity was measured using azocasein as follows.

That is, 0.005 g of azocasein was dissolved in 0.2 M Tris-CI buffer (pH 8.0) and used as a substrate.

In this experiment, 1 unit / 100 μl elastase and 0.8 mM N-Suc- (Ala) 3-ρ-nitroanilide were added to 0.2 M Tris-CI buffer (pH 8.0) for 20 minutes at 25 ° C. Absorbance was measured at nm to quantify the amount of ρ-nitroanilide produced from the substrate.

One unit of elastase inhibitory activity was defined as the amount of the enzyme of elastase that produced ρ-nitroanilide of 1 Чmol per minute.

In addition, inhibitory activity is computed from the following formula.

[Elastase inhibitory activity (%) = (1-B / A) × 100]

     * A: Elastase of untreated group

     * B: Elastase of treatment group

Table 11 Results of Elastase Inhibitory Activity

division Elastase inhibitory activity (%) Example 6 (35% cactus, grape seed 10%) 78.5 Comparative example 3 (17% of palm cactus, grape seed 10%) 53.1 Comparative Example 4 (palm cactus 0%, grape seed 10%) 38.8 Comparative Example 5 (35% cactus, 5% grape seed) 67.4 Comparative Example 6 (35% cactus, grape seed 0%) 58.9 Comparative Example 7 (palm cactus 0%, grape seed 0%) 18.4

As shown in Table 11, the nutrition cream of Example 6 containing the palm cactus fermentation broth and grape seed extract of the present invention was shown to have an excellent elastase inhibitory activity as 78.5%.

In addition, as a result of Comparative Example 3 in which the content of the palm cactus fermentation broth was reduced by half and Comparative Example 4 not added at all, it was confirmed that the palm cactus fermentation broth of the present invention was excellent in elastase inhibitory activity.

Further, as a result of Comparative Example 5 in which the content of grape seed extract was reduced in half and Comparative Example 6 not added at all, it was confirmed that the elastase inhibitory activity of the grape seed extract of the present invention was also excellent.

Therefore, it can be seen that the cosmetic composition containing the fermented cactus fermentation broth and grape seed extract of the present invention has excellent elastase inhibitory activity and excellent anti-wrinkle function.

Experimental Example 4 Measurement of Skin Condition Change of Natural Cosmetic Composition of the Present Invention

A cosmetic composition (skin, lotion, essence serum, nourishing cream) prepared by the method of Examples 4 to 7 of the present invention was prepared.

However, essential oils for each skin type were omitted.

In addition, a cosmetic composition was prepared in the same manner as the four cosmetic compositions, but a comparative composition was prepared by adding purified water instead of palm cactus fermentation broth and grape seed extract.

50 women were selected from 10 women in their 20s and 60s, and the skin conditions (moisture content, skin elasticity, and wrinkles) were measured and recorded before starting the test.

The test subjects were allowed to use the four cosmetic compositions for morning and evening for one month in order of skin, essence serum, lotion, and nourishing cream.

The left side of the face face was used four cosmetic compositions of the present invention, the right side was to use the palm cactus fermentation broth and comparative composition without the addition of grape seed extract.

After 1 month, it measured about moisture retention, skin elasticity, and wrinkles.

Moisturizing power was evaluated by measuring moisture content using a Corneometer (Courase-Khazaka Electronics, Germany; CM 825), skin elasticity and wrinkles were measured using a Cutometer (Courase-Khazaka Electronics, Germany SEM 474).

The results are shown in Table 12 below.

<Table 12> Experimental results of skin condition change

division Cosmetic composition of the present invention Comparative composition Before use after use Before use after use Moisturizing low 32 people 4 people 38 people 25 people usually 10 people 18 people 14 people 13 people high 8 people 38 people 8 people 12 people Skin elasticity bad 30 people 6 people 30 people 21 people usually 11 people 19 people 11 people 13 people good 9 people 25 people 9 people 16 people wrinkle little 12 people 18 people 8 people 9 people usually 17 people 20 people 21 people 23 people many 21 people 12 people 21 people 20 people

As shown in Table 12, the site using the cosmetic composition of the present invention for one month was found to be significantly improved moisturizing and skin elasticity and wrinkles.

However, the portion using the comparative composition without adding the fermented cactus fermentation broth and grape seed extract was slightly improved because it was a cosmetic composition using natural materials, but there was no significant effect.

According to the present invention, a natural cosmetic composition containing palm cactus fermentation broth and grape seed extract is provided.

In addition, the present invention provides a natural cosmetic composition excellent in skin moisturizing, skin elasticity and anti-wrinkle effect.

Claims (4)

delete In the cosmetic composition, Palm cactus fermentation broth comprises 10 to 80% by weight based on the total weight of the composition, grape seed extract comprises 0.1 to 10% by weight relative to the total weight of the composition, The fermented cactus fermentation broth is prepared by preparing rice malt juice and wort, cultivating white mold by producing rice malt juice culture medium, and cultivating white mold by preparing wort culture medium, and then the two types of white mold After culturing together to prepare a yeast culture solution, this yeast culture solution was repeatedly cultured by repeating culture with rice malt juice, and the expanded culture of yeast culture solution was prepared by anaerobic fermentation using wort juice, and then palm palm. The cactus is washed and pulverized, and the sugar is mixed with the ground powder in a weight ratio of 1: 1, and the mixture and water are mixed in a weight ratio of 1:10 to 1:20, and sugar or oligosaccharide is added thereto. Put sugar into 12 to 18 Brix, and add 1 to 5% by weight of the liquid fermentation agent prepared here, compared to the weight of crushed palm cactus, 30, After fermentation at ˜45 ° C. for 5-8 days, the fermentation product was placed in a stirrer and stirred at 50-60 ° C. for 2-7 hours to remove alcohol, and then the filtration was carried out to prepare a fermented cactus fermentation broth. Characterized by Natural cosmetic composition containing palm cactus fermentation broth and grape seed extract. The method of claim 2, Grape seed extract, wash the grape seed, mix the washed grape seed and water in a weight ratio of 1: 1 ~ 1: 3, put it in a water bath and maintain the internal temperature at 50 ~ 60 ℃ heated for 2 to 4 days After extracting, the extract is filtered and put into a cooler characterized in that the grape seed extract prepared by aging for 1 to 10 ℃ for 3 to 7 days, Natural cosmetic composition containing palm cactus fermentation broth and grape seed extract. delete