KR101138157B1 - Natural soap and manufacturing thereof - Google Patents

Abstract

PURPOSE: A natural soap is provided to release pimples and to have excellent skin-moisture effect by using ecklonia cava extract, Dictyota dichotoma extract, Saururus chinensis Baill extract, and green tea extract without using synthetic surfactant. CONSTITUTION: A manufacturing method of a natural soap comprises: a mixing step forming hot water extract mixture by mixing oil mixture, hot water extract and potassium hydroxide; a gelation step for gelatinizing the hot water extract mixture; an aging step for forming soap paste by measuring the hot water extract mixture; a pH control step for randomly controlling the pH of the soap paste to pH 6-7; and ethanol extract mixing step for mixing ethanol extract into the soap paste.

Description

Natural soap and manufacturing method thereof

The present invention relates to a natural soap and a method of manufacturing the same, and preferably, natural soaps having excellent skin moisturizing effect as well as alleviating acne by using Ecklonia cava extract, sesame seed extract, triticale extract and green tea extract, Furthermore, the present invention relates to a method for producing natural soap using an extract obtained by continuously extracting ethanol and hot water.

When attached to human skin, there are various kinds of soaps used to remove waste, sebum, etc. to maintain the cleanliness of the skin, but in general, alkali salts of higher fatty acids are used for soaps used for human cleansing. It is made by saponifying fats and oils.

Therefore, the types of fats and oils, which are the raw materials of soap, are limited to various conditions, and the characteristics of the products vary according to the unique properties of the fats and oils used in soaps. It should be effective. Accordingly, the development trend of soap has recently been promoted in the direction of developing something given a specific function.

However, most commercially available soaps use surfactants synthesized in the petroleum system. Surfactants are a generic term for substances that impart activity between water and oil, skin and wastes to mix water and oil together or to easily remove waste products from the skin. Surfactants with the ability to adsorb to the interface to create bubbles and remove the dirt are left on the skin surface and pores, removing excessive sebum and moisturizing factors that help skin health, making the skin dry and promoting skin aging. In combination with other chemicals, nitrosamine, a carcinogen, can be synthesized and seriously affected. As such, the active agent has a risk of acting as a factor causing cell destruction and various diseases.

On the other hand, acne is a chronic disease of the sebaceous glands that occur mainly on the face, chest, and neck of young men and women, such as adolescent age, and forms comedones, papules, pustules, cysts, or nodules. In severe cases, the lesion may leave a scar. If acne causes severe skin damage, it can also cause mental damage, such as depression or avoiding interpersonal relationships.

The cause of acne has not been elucidated yet, but it is recognized that the following three factors are excluded when genetic and environmental factors are excluded.

The first cause is when sebum is abnormally over secreted. Excess sebum secretion is closely related to the testosterone, a testosterone, which is converted to dihydrotestosterone (DHT) by 5 alpha-reductase in skin cells to enhance the activity of sebaceous cells. It is known to promote sebum secretion (Rosenfield RL and Deplewski D. Role of androgen in the developmental biology of the pilosebaceous unit.The American Journal of Medicine 1995; 16: 80s-88s).

The second cause is when the pores are closed due to hyperkeratinzation of the hair follicles. The produced sebum should be discharged smoothly out of the skin, but the pores are blocked due to the exaggeration of the hair follicles, thereby suppressing the smooth discharge of sebum, and consequently, sebum is stagnated in the hair follicles to form microsurfaces. This condition is the early stage of acne outbreaks.

Third, it is a case of worsening acne lesions by promoting the growth of the acne bacteria resident in the hair follicles propionibacterium acnes (Propionibacterium acnes). As acne bacteria grow in the sebaceous glands, triglycerides that make up sebum are converted into free fatty acids to act as irritants, or to act as a comedonogenic substance. (Sergio Nacht, Cosmetics Toiletries, 101, 47-55, 1986; Wei-Li Lee et al., Infection and Immunity, 35, 71-78, 1982).

In other words, acne bacteria resident in the hair follicles form a pore wall irritant, and the fine cotton cloth is gradually increased in size as the sebum excessive secretion continues to develop into the cotton cloth, and the hair follicle wall becomes thin and is an irritant produced by acne bacteria. Stimulates the thinned hair follicle wall, causing the scalp to deteriorate and progress to papules, pustules, nodules, and scars.

Therefore, the method for evaluating acne treatment and alleviating effects is also used to evaluate sebum secretion control, hyperkeratosis, antibacterial activity against acne bacteria and acne alleviating effects based on the aforementioned mechanism of action (Madli Puhvel, Cosmetics Toiletries, 98, 117-119, 1983; Kathy Perisho et al., J. of Invest.Dermatol, 90, 350-353, 1988; Hyeyoung Kim et al., Korean J. Ginseng Sci., 14, 391-398, 1990; RA Bojar et al., British J. of Dermatology, 132, 204-208, 1995; AM Kligman et al., British J. of Dermatology, 100, 699-702, 1979).

It is a commonly used method for treating acne using drugs. It uses salicylic acid or retinoic acid, a derivative of vitamin A, to suppress or eliminate the formation of keratin in the pores, thereby smoothing the secretion. By treating acne or by using benzoyl peroxide, triclosan, sulfur or tea tree oil preparations to exfoliate the skin and anti-acne Mitigating methods are used. However, salicylic acid preparations may not only have a minimal therapeutic effect, but may also cause skin redness, edema or skin repellence. Retinoic acid preparations may be effective in inhibiting hyperkeratosis, but contact dermatitis, erythema, skin drying and peeling may occur. have. In addition, benzoyl peroxide or trichromic acid formulations have side effects such as allergic contact dermatitis and scars, and severe erythema. On the other hand, the relatively recently introduced azelaic acid (azelaic acid) preparations have reduced drug side effects, but there is a problem that the therapeutic effect is lower than the existing therapeutic products.

Therefore, it is urgent to develop a soap that can alleviate acne without using a synthetic surfactant.

The present invention has been made to solve the above problems, an object of the present invention is to provide a natural soap that can alleviate acne using Ecklonia cava extract, sesame seed extract, three hundred seconds extract and green tea extract.

In addition, it is an object of the present invention to provide a natural soap and a method of manufacturing the same that not only relieve acne but also have an excellent skin moisturizing effect.

In addition, another object of the present invention is to use ethanol extraction and hot water extraction for the Ecklonia cava, three hundred seconds, green tea in sequence to be used in the human body without discarding the useful components.

In order to solve the above problems, the present invention may include the following means.

Natural soap manufacturing method according to the present invention comprises the soap paste forming step of forming a soap paste; A soap making step of making soap; The soap paste forming step may include mixing the oil mixture, the hot water extract, and the caustic mixture to form a hot water extract mixture, a gelling step of forming the hot water extract mixture to be gelled, and aging the gelled hot water extract mixture. By further comprising a aging step to form a soap paste, the soap production step, characterized in that it further comprises an ethanol extract mixing step of mixing the ethanol extract to the aged soap paste.

The soap production step is characterized in that it further comprises a pH adjustment step to form a pH of the soap paste to pH 6 ~ 7.

The ethanol extract is characterized in that any one selected from Ecklonia cava ethanol extract, sesame net ethanol extract, three hundred seconds ethanol extract and green tea ethanol extract extracted by ethanol.

The hot water extract is characterized in that the Ecklonia ethanol extract, 300 seconds ethanol extract and green tea ethanol extract are extracted by purified water, respectively, to form Ecklonia hot water extract, 300 seconds hot water extract, green tea hot water extract.

The oil mixture is characterized in that it is formed of one or two or more selected from coconut oil, olive oil, camellia oil, castor oil, sunflower oil, soybean oil.

The natural soap according to the present invention contains Ecklonia chinensis ethanol extract, sesame seed ethanol extract, three hundred sec ethanol extract and green tea ethanol extract, and Ecklonia hot water extract, three hundred seconds hot water extract, green tea hot water extract as an active ingredient.

Acne-relieving natural soap according to the present invention contains Ecklonia ethanol extract, sesame seed extract ethanol extract, three hundred seconds ethanol extract and green tea ethanol extract, Ecklonia hot water extract, three hundred seconds hot water extract, green tea hot water extract.

The present invention uses the Ecklonia cava extract, sesame seed extract, three hundred seconds extract and green tea extract to alleviate acne.

In addition, the present invention not only relieves acne but also has an excellent skin moisturizing effect.

The present invention provides a manufacturing method to prevent the useful components are discarded by using ethanol extraction and hot water extraction continuously in the present invention.

Hereinafter, the present invention will be described in detail.

On the other hand, the 'extract' referred to in the present invention is a concept that includes all of the general extract is used as a concept including the hot water extract and ethanol extract, in particular 'hot water extract' refers to that extracted by the hot water extraction method using distilled water In addition, "ethanol extract" refers to an extract using a polar solvent selected from a lower alcohol having 1 to 4 carbon atoms or a mixed solvent thereof, preferably ethanol.

The present invention can provide a natural soap that can alleviate acne without using a chemical surfactant by using Ecklonia cava extract, sesame seed extract, triticale extract and green tea extract, and excellent skin moisturizing effect A soap and its manufacturing method can be provided.

Ecklonia cava used in the present invention (Ecklonia cava) is a perennial seaweed belonging to the kelp family of brown algae. Distributed on the south east coast, south coast and Jeju island, it is the most mature in spring. The stem is a thick cylinder, and the upper part is the flat leaf part. In the spring, between the stem and the fronds, a new frond grows for 3-4 years. The color is brown but when it is dry it becomes black and the quality is leather. Dried Ecklonia is used as a raw material for alginic acid or as a fertilizer for bulb crops.

The three hundred sec used in the present invention is a perennial herb belonging to the three hundred genus (Saururaceae), and three to three leaves on the upper part of this plant stem is called three hundred sec (三 白草). Saururaceae is distributed in Korea, Japan, China, North America, Mexico, etc., and is classified into 5 genus 7 species as follows [Wu.CY, Acta Phytotaxon, Sinica, 6, 222 (1957)] Has been used for detoxification, rubella, stool, hypertension, diuresis, and is also used for the treatment of customary arthritis. Its main ingredients include Quercetin, Quercitrin, Isoquercitrin, and Hyperin in the leaves, and methyl-n-nonylketone (Essential oil) in the outpost. Methyl-n-nonyl ketone) contains rutin in the stem (Chinese medicine dictionary). These quercetin, quelcitrin, isocuelcitrin, hyphenin, rutin and the like are flavonoids (flovonoides), flavonoids such as polyphenols (polyphenols) have an antioxidant action, that is, free radical scavenging action. Zheng et al. Presented flavonoid assays in the outpost. (徐 (, 沙 世 炎: 中草藥 有效 成分 分析 法: 下 冊, 東京, 34 (1984))

Green tea used in the present invention (Green-tea, Camellia teaceac) is a non-alcoholic beverage containing caffeine (caffeine) along with coffee, cocoa and black tea is one of the favorite beverages that Koreans have enjoyed since ancient times. Green tea is known to supply vitamins and minerals, satisfy tastes and flavors. Among 100 mg of dry green tea leaves, 13 mg of carotene, 65.4 mg of vitamin E, and It is reported that dietary fiber contains 10.6 mg and the like and is rich in minerals such as Ca, Fe and K. The main components of the astringent taste of these green teas are catechins, which are polyphenols, which are epicatechin, epigallocatechin, epicatechin gallate and epigallo, depending on their derivatives. Catechin gallate (Epigallocatechin gallate) and the like. Catethin is about 8-20% of dry green tea, and is soluble in water when it is boiled with water. The bitterness of green tea is mainly due to caffeine, which is known to have awakening, diuretic and fatigue recovery effects. The unique characteristic ingredient is theanine, which is double-glutamic acid ethylamide. Accounts for about half, and contains more than 20 amino acids. On the other hand, green tea contains various physiologically active substances. Especially, catechins have the ability to prevent blood clot formation and atherosclerosis, inhibit the production of lipid peroxide and prevent fatty acidization by reducing LDL cholesterol in the blood. It turns out and is used also as a natural antioxidant. In addition, green tea is known to prevent food poisoning, as well as anti-aging and inhibits the growth of cancer and has a carcinogenic function as well as an anti-toxin action to neutralize the toxin. In particular, it was found that Epigallocatechin gallate contained in green tea is the main active substance, and it has 25 times higher antioxidant activity than vitamin E and 100 times higher than vitamin C, which can be used for preventing or preserving lipid oxidation of aquatic foods. In recent years, it has been proven to be effective in removing heavy metals.

Dictyota dichotoma (Hudson) Lamourorux (sensus in Okamura) of the present invention used in the present invention is taxonomically classified as Phaeophyta order Dictyotales, family Dictyotaceae, Tribe

Dictyoteae), this species grows entangled with each other at depths of 1 to 5 m in the subsea zone of the south coast including the east coast and Jeju island. They are so thin that they are unusually shaken by the flow of waves or algae, they are pale grey-brown in color, and have a blue fluorescence throughout their bodies, making them clearly distinguishable from other nets in the open air.

Natural soap manufacturing method according to the present invention, the soap paste forming step of forming a soap paste; Soap production step of producing a soap.

The soap paste forming step further includes a mixing step, a gelling step, and a aging step.

The mixing step is a step of mixing the oil mixture, hot water extract and caustic (potassium hydroxide) to form a hot water extract mixture, generally using a caustic when preparing natural soap, conventionally when water is dissolved caustic However, the present invention uses a hot water extract containing a component such as polysaccharide or alginic acid soluble in water to dissolve the caustic to form a soap paste, so that the natural soap according to the present invention is more moisturizing effect.

On the other hand, the oil mixture may be used in a variety of oils, coconut oil, olive oil, camellia oil, castor oil, sunflower oil, soybean oil is formed of one or two or more selected from various composition ratios, preferably the oil Silver is suitably mixed at 0.1 to 8% with respect to the composition of the whole natural soap, more preferably it is preferable to contain all together coconut oil, olive oil, camellia oil, castor oil, sunflower oil, soybean oil.

On the other hand, the hot water extract is added to the purified water of the Ecklonia cava ethanol extract, three hundred seconds ethanol extract and green tea ethanol extract to be described below in 10 times the amount of purified water and stirred for 2-5 hours at 70 ~ 100 ℃ preferably 90 ℃ It is preferable to extract the Ecklonia cava hot water extract, three hundred seconds hot water extract, green tea hot water extract, wherein the Ecklonia cava hot water extract, three hundred seconds hot water extract, green tea hot water extract is contained in 0.1 to 3% by weight relative to the total natural soap composition Is suitable. By hot water extraction by such purified water, substances such as polysaccharides that are well dissolved in water are highly extracted.

On the other hand, the extract of the present invention is that the hot water extraction is made after the ethanol extraction, the hot water extraction is applied to the heat is applied to the weak components of the Ecklonia cava, three hundred seconds, the green tea is a problem that the deformation and hot water Since the extraction is made first, the problem of changing the moisture content of the Ecklonia cava, three hundred seconds, green tea occurs, it is preferable to perform the hot water extraction after the ethanol extraction first to prevent this.

Further, by using the ethanol extract to obtain the useful components first to use in natural soap, by extracting the hot water for ethanol extracted Ecklonia cava, three hundred seconds, green tea used in the present invention, the useful components are not discarded and used in the human body To be possible.

In the gelling step, the hot water extract mixture is placed on a hot plate and stirred with a hand blender until it is jelly at 70 to 80 ° C.

The aging step is a step of completing the soap paste by sealing the gelled hot water extract mixture at various temperatures, preferably at room temperature, and then aging for 1 to 3 months to add a substance having an antiseptic effect such as sugar. It may be.

On the other hand, the soap production step may further include a pH adjustment step, ethanol extract mixing step.

The pH control step is to form a pH of the aged soap paste 6 ~ 7 using vinegar, etc. to alleviate the skin irritation, the aged soap paste is a predetermined amount of purified water to the aged soap paste It is also possible to use the diluted.

The ethanol extract mixing step is to add the ethanol extract extracted by ethanol to the aged soap paste, the ethanol extract may be prepared as follows.

After washing the Ecklonia cava, three hundred seconds, green tea, sesame seed meal with water, and then dried in the shade or sunlight, it is ground to a suitable size.

First, pulverized Ecklonia cava, three hundred gree, green tea, and sesame seed meal are prepared by using a polar solvent selected from a lower alcohol having 1 to 4 carbon atoms or a mixed solvent thereof, preferably ethanol, and more preferably 70% alcohol. After leaching for 20 to 40 days by filtration to obtain the Ecklonia cava ethanol extract, three hundred s ethanol extract, green tea ethanol extract, sesame seed ethanol extract, respectively. By ethanol extraction, bioactive substances such as lipids, which are insoluble in water, are highly extracted.

Furthermore, Ecklonia cava ethanol extract, three hundred s. Ethanol extract, green tea ethanol extract, and sesame seed ethanol extract were added to purified water, stirred at 70 to 100 ° C., preferably at 90 ° C. for 2 to 5 hours, and then filtered. Hot water extract, three hundred seconds hot water extract, green tea hot water extract, sesame seed based hot water extract will be prepared. By hot water extraction by such purified water, substances such as polysaccharides that are well dissolved in water are highly extracted.

 On the other hand, Ecklonia cava ethanol extract, three hundred seconds ethanol extract, green tea ethanol extract, sesame net ethanol extract is preferably contained in 0.1 to 3% by weight based on the total soap composition. When the Ecklonia cava, iris and green tea extract are included in less than 0.1% by weight, the moisturizing effect of the shampoo composition is insignificant, and when added in excess of 3.0% by weight, the synergistic effect due to the overinjection is not economical. It can have a negative impact.

On the other hand, natural soap according to the present invention may be formed in a liquid or solid phase, Ecklonia chinensis ethanol extract, sesame seed ethanol extract, three hundred seconds ethanol extract and green tea ethanol extract, persimmon hot water extract, three hundred seconds hot water extract, green tea hot water extract It is contained as an active ingredient.

In addition, the natural soap according to the present invention contains an Ecklonia ethanol extract, a sesame seed ethanol extract, three hundred seconds ethanol extract and green tea ethanol extract, and the Ecklonia hot water extract, three hundred seconds hot water extract, green tea hot water extract as an active ingredient to relieve acne In addition, it is excellent for skin moisturizing.

The components included in the natural soap of the present invention include components commonly used in addition to the active ingredient, and may include, for example, conventional auxiliaries such as antioxidants, stabilizers, solubilizers, pigments and flavors, and carriers. It is also possible to contain lavender essential oil, tea tree essential oil, rosemary essential oil, peppermint, menthol and the like within the scope of not impairing the effect of the invention.

In addition, the present invention may be prepared in any formulation conventionally prepared in the art, including, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing, It may be formulated as an oil, powder foundation, emulsion foundation, wax foundation and spray, shampoo, but is not limited thereto. More specifically, it may be prepared in the form of liquid soap, flexible lotion, nourishing lotion, nourishing cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.

Hereinafter, the present invention will be described in more detail with reference to Examples and Experimental Examples. The following Examples and Experimental Examples are only for more specifically describing the present invention, and the scope of the present invention is not limited by them according to the gist of the present invention. It will be obvious to him.

Preparation Example 1 Preparation of Ecklonia cava Ethanol Extract and Ecklonia cava Water Extract

After washing the Ecklonia cava twice in fresh water to remove salt and foreign substances, dried in the sun, dried and finely pulverized, then leached into 10% 70% alcohol of crushed Ecklonia cava for 30 days and then filtered to prepare Ecklonia ethanol extract. The leaching filtration finished Ecklonia cava ethanol extract was placed in 10 times the amount of purified water and heated at 90 ° C. for 3 hours with stirring, followed by filtration to prepare Ecklonia cava extract.

Preparation Example 2-Preparation of 300 sec Ethanol Extract and 3 300 sec Hot Water Extract

The dried three hundred seconds powder is leached in 10% of 70% alcohol for about 30 days and then filtered to prepare a three hundred seconds ethanol extract. The leaching was added to purified water in an amount of 10 times the weight of the extracted three hundred seconds ethanol extract was stirred at 90 ℃ for 3 hours, followed by filtration to prepare a three hundred seconds hot water extract.

Preparation Example 3-Preparation of Green Tea Ethanol Extract and Green Tea Hot Water Extract

Purchasing dried green tea leaves, finely pulverized, and leaching in 70% alcohol of 10 cultures of green tea for about 30 days and filtered to extract green tea ethanol extract. The leached tea green tea ethanol extract was added to 10 times the weight of purified water, stirred at 90 ° C. for 3 hours, heated, and filtered to prepare green tea hot water extract.

Preparation Example 4 Preparation of True Net Ethanol Extract

Wash the sesame seeds in fresh water twice to remove salt and foreign substances, dry them in the sun, dry them finely, grind them finely, leach them in 10% of 70% alcohol for about 30 days, and then filter and prepare sesame seeds ethanol extract.

Preparation Example 5-Preparation of Soap Paste

To prepare natural soap, first prepare soap paste.

1. Weigh oil according to Table 1.

Configuration Usage (g) % Of total soap Coconut (g) 300 4.01 olive 225 3.01 Grapeseed oil 225 3.01 Camellia oil 100 1.34 Palma Christi 200 2.68 Sunflower oil 150 2.01 Soybean oil 300 4.01

2. Measure the hot water extract of Ecklonia cava, green tea, and 300 sec prepared in Preparation Examples 2, 3, and 4 according to Table 2 to form a hot water extract mixture.

Configuration Usage (g) % Of total soap Ecklonia cava extract 100.0 1.34 Green Tea Hot Water Extract 100.0 1.34 Three hundred seconds hot water extract 154.0 2.06

3. Weigh 354g of caustic fish and dissolve it in hot water extraction mixture of 2.

4. Mix the oil and caustic melted hot water extract mixture between 70 ℃ ~ 80 ℃.

5. Place 4 on hotplate, set temperature to 70-80 ° C and stir with hand blender until jelly.

6. Once the jelly has formed, put it down on a hot plate, add sugar-dissolved water and stir well.

7. After sealing, aged for 3 months to prepare a soap paste.

8. Put brown rice vinegar in the market into matured soap paste and prepare soap paste by adjusting pH to pH 6 ~ 7.

[Manufacture example 6]-Soap production

Including the extract and soap paste prepared in Preparation Examples 1 to 5 to prepare a natural shampoo of Example 1 in the composition ratio of Table 3, the commercial liquid soap was commercially available in Comparative Example 1.

Configuration Example 1 (% by weight)  Example 3 (% by weight) Soap Paste 30.75 30.75 Ecklonia ethanol extract 1.34 0.8 Green Tea Ethanol Extract 1.34 1.5 Ethanol Extract of 300C 1.34 1.5 True Net Ethanol Extract 0.67 1.0 Lavender Essential Oil 0.27 0.46 Tea Tree Essential Oil 0.20 0.27 Rosemary 0.13 0.08 Purified water To 100 To 100

Experimental Example 1

In order to determine the degree of skin irritation for the shampoo composition according to the present invention, a skin test was carried out for Example 1 and Comparative Example 1. For skin testing, a small sample was placed on the upper arm against five women from each age group from five to ten years of age. After 1 hour, the skin reaction was first visually examined, and after 24 hours, the second visual The test results are shown in Table 5 below.

division Skin irritation degree Example 1 1st: ○, 2nd: ○ Comparative Example 1 1st: △, 2nd: △

(○: no skin cotton, △: slight erythema appears, x: clear erythema appears)

Looking at the results of Table 4, in the case of the typical liquid soap of Comparative Example 1 was found to be mild erythema irritated to the skin, while the natural soap composition of the present invention of Example 1 can be used safely without irritation to the skin Can be.

[Experimental Example 2]

The moisturizing effect experiment for Example 1, Comparative Example 1 was carried out. For this purpose, 20 male and female adults were selected. The ages ranged from 25 to 55 years with 9 males and 11 females. The subjects were divided into two groups of 10 people each, and the composition of Example 1 was used in the first group, and the composition of Comparative Example 1 was used in the second group, so that the face was washed once every morning, lunch, and dinner in the usual manner for two months. After that, the moisturizing effect of the skin was evaluated by a 5-point scale method. The results are shown in Table 5 below.

division Moisturizing effect Example 1 4.5 Comparative Example 1 3.3

(1: Very bad, 2: Bad, 3: Normal, 4: Good, 5: Very good)

Looking at the results of Table 5, it can be seen that the natural soap composition of the present invention is significantly effective in the moisturizing effect when compared with Comparative Example 1 which is a conventional liquid soap.

Experimental Example 3 Sebum Secretion Inhibitory Effect

The sebum secretion inhibitory effect was tested by performing experiments on the inhibition of 5 alpha-reductase activity of Example 1 and Comparative Example 1 as follows.

Testosterone (3H-1,2,4,5,6,7-) bound to dithiothreitol, NADPH, and radioisotopes in 30 µl of Phosphate Buffered Saline (PBS / pH 6.6) 10 μl of testosterone, 40 μl of 5 alpha-reductase enzyme extract and 20 μl of the components of Examples 1, 2 and Comparative Example 1 were mixed at 0.1% concentration, respectively. Subsequently, after reacting in an incubator for 2 minutes, the reaction solvent is blown out, the remaining material is dissolved in the developing solvent, and the thin layer chromatogram (TLC) is developed. The quantitative analysis was carried out to measure the conversion rate of testosterone to dihydrotestosterone by 5 alpha-reductase, and the result was compared with the control group (conversion rate when no extract was added) according to the following equation (1) Inhibition rate (%) for 5 alpha-reductase enzymes of 1 and Comparative Example 1 was calculated and the results are shown in Table 6 below.

[Equation 1]

% Inhibition = (dihydrotestosterone conversion rate of testosterone in samples without Cryptotansinone) × 100 / (dihydrotestosterone conversion rate of testosterone in samples added with Cryptotansinone)

% Inhibition of activity of alpha-reductase Example 1 89.2 Comparative Example 1 39.4

As can be seen in Table 6, Example 1 is very effective in inhibiting sebum secretion, it is determined that acne can be prevented and alleviated.

Experimental Example 4 Antimicrobial Activity

In order to measure the antimicrobial activity of the Propionibacterium acnes of Example 1 and Comparative Example 1, the Propionibacterium acnes stored in a bacterium in a bacteriostatic state 3 days before the test, the BHI (Brain Heart Infusion) solid After incubation on the plate medium, Example 1 and Comparative Example 1 was inoculated with propionibacterium acnes pre-cultured in a non-Echi'ai solid plate medium containing a certain concentration. Then, the minimum inhibitory concentration (MIC) was determined by measuring the number of propionibacterium acnes after 3 days, and is shown in Table 7.

Minimum Inhibition Concentration (MIC (Mole)) Example 1 95 ppm Comparative Example 1 421ppm

As can be seen in Table 7, the natural soap according to the present invention was confirmed that the growth inhibitory effect of Propionibacterium acnes was excellent, thereby finding that it is effective in preventing and alleviating acne, thus completing the present invention. .

Claims (8)

A soap paste forming step of forming a soap paste;
A soap making step of making soap;
The soap paste forming step,
A mixing step of mixing the oil mixture, the hot water extract, and the caustic to form a hot water extract mixture,
A gelation step of forming the hot water extract mixture to be gelled;
Further comprising the maturation step of forming the soap paste by aging the gelled hot water extract mixture,
The soap production step,
Natural soap production method characterized in that it further comprises the step of mixing the ethanol extract to the aged soap paste ethanol extract. The method of claim 1, wherein the soap manufacturing step,
Natural soap production method further comprises a pH adjusting step of forming the pH of the soap paste to pH 6 ~ 7. The method of claim 1, wherein the ethanol extract,
Method for producing natural soap, characterized in that any one or more selected from the ethanol extract extracted from ethanol, sesame net ethanol extract, three hundred seconds ethanol extract and green tea ethanol extract. The method of claim 3, wherein the hot water extract,
And extracting the Ecklonia cava ethanol extract, three hundred sec ethanol extract and green tea ethanol extract with purified water, respectively, to form persimmon hot water extract, three hundred sec hot water extract, and green tea hot water extract. The method of claim 1, wherein the oil mixture,
Natural soap manufacturing method characterized in that formed by one or more selected from coconut oil, olive oil, camellia oil, castor oil, sunflower oil, soybean oil. Natural soap containing Ecklonia chinensis ethanol extract, sesame seed ethanol extract, three hundred ethanol extract and green tea ethanol extract, and Ecklonia cava hot water extract, three hundred sec hot water extract, green tea hot water extract as active ingredients. Natural soap for alleviating acne, containing Ecklonia chinensis ethanol extract, sesame seed ethanol extract, three hundred ethanol extract and green tea ethanol extract, and Ecklonia hot water extract, three hundred sec hot water extract, green tea hot water extract as active ingredients. Natural soap prepared by the method of any one of claims 1 to 5.