KR102001069B1 - A composition for improving inflammatory skin disease containing evergreen woody species - Google Patents

Abstract

The present invention relates to a composition for improving inflammatory skin diseases, which comprises an extract of an herbaceous woody plant. More specifically, the present invention relates to a composition for improving inflammatory skin diseases, which comprises Elaeocarpus sylvestris var. a cosmetic composition, and a health functional food, which comprises an ellipticus (Thunb.) H. Hara extract, a Cleyera japonica Thunb. extract, or a combination thereof as an active ingredient.

Description

Technical Field [0001] The present invention relates to a composition for improving inflammatory skin diseases, which comprises an extract of a woody plant of the genus Arthropoda,

The present invention relates to a composition for improving inflammatory skin diseases, which comprises an extract of an herbaceous woody plant. More specifically, the present invention relates to a composition for improving inflammatory skin diseases, which comprises Elaeocarpus sylvestris var. a cosmetic composition, and a health functional food, which comprises an ellipticus (Thunb.) H. Hara extract, a Cleyera japonica Thunb. extract, or a combination thereof as an active ingredient.

Skin is a human body that protects the body from external stimuli and environmental changes and transmits tactile senses, and plays a symbolic role of youth, health, and sexual ability. In modern society, skin is not only cosmetic beauty but also its own expression, and it is an important factor that determines fashion trend with costume and makeup. The causes of skin abnormalities include genetic factors, stress, hormonal abnormalities, parasitic parasites on the skin, and harmful skin infections. In healthy skin, beneficial bacteria are present at a ratio of 80% and harmful bacteria are present at a ratio of 20%, and the skin is maintained to be slightly acidic (pH 5.5). In this case, the beneficial gynecological secretion from the sweat secreted by the immune system is combined with immunity. In addition, when the acid is weak, the healthy skin keeps the skin moist, prevents water leaks from the skin as a barrier to the body, and helps the vitamins biosynthesis. Incorrect cleaning habits, long-term use of chemical or oil products, and imbalance of testosterone due to biorhythm and physiological changes may aggravate sebaceous glands and exacerbate sebum secretion. If the hair follicle is clogged with an abnormal angle to the hair cell wall cells by the excessive secreted sebum, sebum will not be discharged and it will stagnate. Thus, the skin becomes homogenous and the anaerobic bacteria, aerobic bacteria, fungi and the like are reproduced by creating a condition for the growth of the microorganisms in the pores. At this time, Propionibacterium As acnes secretes lipase, triglyceride of sebum component changes into free fatty acid. After the hair follicles are densely packed around the hair follicles, the hair follicles are destroyed and the contents of the hair follicles are infiltrated into the dermis and inflammation occurs.

Staphylococcus aureus , Staphylococcus. epidermidis and Propionibacterium acnes , yeast Malassezia and furfur . The strain also causes scalpitis, seborrheic dermatitis, eczema, dandruff, itching, hair loss and acne. This phenomenon is not directly related to life, but it is a serious problem because it causes low psychological-social impacts such as self-evaluation, self-confidence, loss of self-esteem, relationship between people and social position.

Currently, steroid preparations, topical immunosuppressants, topical antibiotics, and antihistamines are currently used as therapeutic agents for inflammatory skin diseases. However, when the drug preparation is used for a long period of time, Side effects such as palpitation may occur. In particular, when steroids are applied to a wide range of sites, medication can be systemically absorbed through the skin, which may interfere with growth of the child and systemic side effects such as osteoporosis can occur in adults. Therefore, it is necessary to develop a composition derived from a natural substance, which has few side effects and is safe for the human body and is excellent in an inflammatory skin disease-improving effect.

Therefore, the inventors of the present invention have found that, in order to find a substance having an inflammatory skin disease improving effect and a lesser side effect on human body, the present inventors have found that Elaeocarpus sylvestris var. ellipticus (Thunb.) H. Hara ] extracts or Cleyera japonica Thunb. extract showed significantly improved antimicrobial activity against Staphylococcus aureus, Staphylococcus epidermidis, and Propionibacterium acnes , The present invention has been accomplished by discovering the facts.

Precedent literature

Korean Patent No. 10-1731794

The present invention relates to a pharmaceutical composition for preventing or treating an inflammatory skin disease comprising an extract of an herbaceous herbaceous plant as an active ingredient, a cosmetic composition for improving inflammatory skin diseases including an herbal botanical extract, and an inflammatory And to provide a health functional food for improving skin diseases.

As used herein, the term " anti-inflammatory " refers to resistance to inflammation, which is the pathological condition of an abscess formed by the invasion of external infectious agents such as bacteria, fungi, viruses, it means.

The term "active ingredient ", as used herein, refers to a component which alone exhibits the desired activity or which itself can exhibit activity with a carrier that is not active.

As used herein, the term "unbranched woody plants" refers to leaves, branches, roots, or mixtures thereof of an evergreen woody plant to be extracted, regardless of the extraction method, in an alcohol of 1 to 5 carbon atoms such as methanol, ethanol, butanol, Ethyl acetate, chloroform, dichloromethane, water or a mixed solvent thereof, and an extract obtained by fractionating the extract with the above-listed solvents. As long as it is extracted through the step of immersing the leaves, branches, roots, or a mixture thereof in the extraction solvent, the extraction method may be any method such as freezing, refluxing, heating, It should be understood that the invention can be applied. However, the above-mentioned "evergreen woody plant" is preferably obtained by extracting the leaves, branches, roots or a mixture thereof of water, alcohols or a mixed solvent thereof, which is an extract of concentrated, Or an extract of a solid form, an extract of a fraction solvent layer obtained by fractionating the solid extract with water, hexane, water and dichloroform, water and ethyl acetate or water and butanol, or a solid form of the extract, Followed by suspending, followed by fractionation using hexane, dichloroform, ethyl acetate and butanol. Here, "sequential fractionation" means that the fraction of the water layer is continuously used after fractionation and is fractionated into the solvent of the above-mentioned order.

According to a first embodiment,

The present invention provides a pharmaceutical composition for the prevention or treatment of inflammatory skin diseases including an herbaceous plant extract . The extract of the herbaceous herbaceous plant is a plant extract of Elaeocarpus sylvestris var. ellipticus (Thunb.) H. Hara ] extract, Cleyera japonica Thunb. extract, or a combination thereof.

In the pharmaceutical composition according to the present invention, the inflammatory skin disease may be any one selected from the group consisting of atopic dermatitis, contact dermatitis, seborrhea and acne, But is not limited thereto.

In the pharmaceutical compositions according to the invention, the sangrokseong woody plant extracts star fatigue nose kusu aureus (Staphylococcus aureus), star fatigue nose kusu epidermidis (Staphylococcus epidermidis), and propionic sludge tumefaciens arc Ness (Propionibacterium acnes Can be inhibited.

In the pharmaceutical composition according to the present invention, the extract of the herbicidal woody plant may be a leaf extract, an eggplant extract or a root extract of an evergreen woody plant. Preferably, the herbaceous plant extract is an evergreen tree leaf extract.

In the pharmaceutical composition according to the present invention, the extract of the herbicidal woody plant may be an alcoholic extract of an evergreen woody plant. The alcohol may be a lower alcohol having 1 to 4 carbon atoms. For example, the alcohol may be methanol, ethanol, n-propanol, iso-propanol, n-butanol, iso-butanol or tert-butanol. Preferably, the alcohol may be methanol or ethanol.

In the pharmaceutical composition according to the present invention, the above-mentioned extract of the herbaceous herbaceous plant is obtained by extracting a methanol extract of the herbaceous herbaceous plant in a fraction of water, hexane, water and dichloromethane, water and ethyl acetate or water and butanol Extract of layer; Or b) an extract obtained by successively fractionating using hexane, dichloroform, ethyl acetate and butanol. Preferably, the herbaceous plant extract may be a butanol fraction of a methanol extract of an evergreen tree plant.

The pharmaceutical composition of the present invention can be formulated by adding a pharmaceutically acceptable carrier. For formulation, refer to Remington's Pharmaceutical Science, Mack Publishing Company, Easton PA. The pharmaceutically acceptable carrier means those conventionally used in the manufacture of a pharmaceutical composition for a person skilled in the art to which the present invention belongs. For example, there may be mentioned lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methylcellulose, microcrystalline cellulose, Water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, and mineral oil. Pharmaceutically acceptable carriers also include diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrants, surfactants, and the like.

The amount of the herbicidal extract of the herbaceous plant contained in the pharmaceutical composition of the present invention varies depending on the condition and body weight of the patient, the severity of the disease, the drug form, the administration route and the period, but may be appropriately selected depending on the case. For example, the herbicidal extract of the herbicide may be administered at a dose of 0.0001 to 1000 mg / kg per day, preferably 0.1 to 1000 mg / kg per day, and the application may be applied once a day or divided into several times . In addition, the pharmaceutical composition may contain 0.0001 to 50% by weight of the above-mentioned non-specific woody plant extract, based on the total weight of the composition. The pharmaceutical composition may be applied to mammals such as humans by various routes, for example, transdermal, oral, intravenous, intramuscular, or subcutaneous injection.

The pharmaceutical composition of the present invention may contain at least one active ingredient which exhibits improvement, alleviation, treatment or prevention of skin inflammation in addition to the extract of the herbicide of woody plants.

The pharmaceutical composition of the present invention can be used alone, for improving, alleviating, treating or preventing skin inflammation, or in combination with methods using surgery, hormone therapy, drug therapy and biological response modifiers.

According to a second embodiment,

The present invention provides a cosmetic composition for improving inflammatory skin diseases, which comprises an extract of an herbaceous herbaceous plant, wherein the herbaceous plant extract of Eucalyptus is Elaeocarpus sylvestris var. ellipticus (Thunb.) H. Hara ] extract, Cleyera japonica Thunb. extract, or a combination thereof.

In the cosmetic composition according to the present invention, the inflammatory skin disease may be any one selected from the group consisting of atopic dermatitis, contact dermatitis, seborrhea and acne, But is not limited to.

In the cosmetic composition according to the present invention, the sangrokseong woody plant extracts star fatigue nose kusu aureus (Staphylococcus aureus), star fatigue nose kusu epidermidis (Staphylococcus epidermidis), and propionic sludge tumefaciens arc Ness (Propionibacterium acnes) Can be inhibited.

In the cosmetic composition according to the present invention, the extract of the herbicide of the present invention may be a leaf extract, an eggplant extract or a root extract of an evergreen woody plant. Preferably, the herbaceous plant extract is an evergreen tree leaf extract.

In the cosmetic composition according to the present invention, the extract of the herbicidal woody plant may be an alcoholic extract of an evergreen woody plant. The alcohol may be a lower alcohol having 1 to 4 carbon atoms. For example, the alcohol may be methanol, ethanol, n-propanol, iso-propanol, n-butanol, iso-butanol or tert-butanol. Preferably, the alcohol may be methanol or ethanol.

In the cosmetic composition according to the present invention, the above-mentioned extract of the herbaceous herbaceous plant is obtained by extracting a methanol extract of an evergreen woody plant with a) one fraction solvent layer obtained by fractionating water with hexane, water and dichloroform, water and ethyl acetate or water and butanol ≪ / RTI > Or b) an extract obtained by successively fractionating using hexane, dichloroform, ethyl acetate and butanol. Preferably, the herbaceous plant extract may be a butanol fraction of a methanol extract of an evergreen tree plant.

The cosmetic composition of the present invention can be used as a cosmetic composition for cosmetics such as softening lotion, convergent lotion, nutritional lotion, nutritional cream, massage cream, essence, eye cream, eye essence, cleansing cream, cleansing foam, cleansing water, pack, powder, body lotion, , A body essence, a makeup base, a foundation, a hair dye, a shampoo, a rinse and a body cleanser.

The cosmetic composition of the present invention can be prepared in various forms according to a conventional cosmetic preparation method using the above-mentioned extract of the herbaceous woody plant, and can be used as a stabilizer, a dissolving agent, a vitamin, a pigment, , ≪ / RTI > and the like. When the cosmetic composition is prepared in the form of a perfumed product containing the gosaricin alcohol extract, a shampoo, a hair lotion, a hair cream, a hair gel, etc., it can be diluted with a usual cleansing solution, a converging solution and a moisturizing solution. The cosmetic composition of the present invention is particularly preferably prepared in the form of a lotion, lotion, cream, or essence.

In the cosmetic composition of the present invention, the extract of the herbicide of the present invention may be added to the cosmetic composition in an amount of 0.1 to 10 wt% based on the total weight of the cosmetic composition, and may be added in an amount of 0.001 to 5 wt% , Preferably 0.01 to 3% by weight, based on the total weight of the cosmetic composition.

According to a third embodiment,

The present invention provides a food composition for improving inflammatory skin diseases, which comprises an extract of a herbaceous herbaceous plant. The herbaceous plant extract of the present invention can be used as an extract of Elaeocarpus sylvestris var. ellipticus (Thunb.) H. Hara ] extract, Cleyera japonica Thunb. extract, or a combination thereof.

In the food composition according to the present invention, the inflammatory skin disease may be any one selected from the group consisting of atopic dermatitis, contact dermatitis, seborrhea and acne, But is not limited to.

In the food composition according to the invention, the sangrokseong woody plant extracts star fatigue nose kusu aureus (Staphylococcus aureus), star fatigue nose kusu epidermidis (Staphylococcus epidermidis), and propionic sludge tumefaciens arc Ness (Propionibacterium acnes) Can be inhibited.

In the health functional food according to the present invention, the above-mentioned non-specific woody plant extract may be a leaf extract, an eggplant extract or a root extract of an evergreen woody plant. Preferably, the herbaceous plant extract is an evergreen tree leaf extract.

In the health functional food according to the present invention, the extract of the herbicidal woody plant may be an alcoholic extract of the herbaceous woody plant. The alcohol may be a lower alcohol having 1 to 4 carbon atoms. For example, the alcohol may be methanol, ethanol, n-propanol, iso-propanol, n-butanol, iso-butanol or tert-butanol. Preferably, the alcohol may be methanol or ethanol.

In the health functional food according to the present invention, the abovementioned extract of the herbaceous herbaceous plant is obtained by extracting a methanol extract of an herbaceous herbaceous plant in a fraction of water, hexane, water and dichloromethane, water and ethyl acetate or water and butanol, Extract of layer; Or b) an extract obtained by successively fractionating using hexane, dichloroform, ethyl acetate and butanol. Preferably, the herbaceous plant extract may be a butanol fraction of a methanol extract of an evergreen woody plant.

The health functional food of the present invention can be used as a nutritive, a vitamin, a mineral (electrolyte), a flavoring agent such as a synthetic flavor agent and a natural flavor agent, a coloring agent and an aging agent (cheese, chocolate etc.), a pectic acid and its salt, A salt, an organic acid, a protective colloid thickener, a pH adjusting agent, a stabilizer, a preservative, a glycerin, an alcohol, a carbonating agent used in a carbonated beverage, and the like, as well as the production of natural fruit juice and fruit juice drinks and vegetable drinks ≪ / RTI > These components may be used independently or in combination.

The health functional food of the present invention may be any one of meat, sausage, bread, chocolate, candy, snack, confectionery, pizza, ramen, gum, ice cream, soup, beverage, tea, functional water, Lt; / RTI >

The health functional food of the present invention may further contain food additives and the suitability of the food additives as "food additives" is to be determined in accordance with the General Rules and General Test Methods approved by the Food and Drug Administration, Shall be determined according to the relevant standards and standards. Examples of the products listed in the above-mentioned "food additives" include natural products such as ketones, chemical products such as glycine, potassium citrate, nicotinic acid and cinnamic acid, coloring matter, licorice extract, crystalline cellulosic, high- , A sodium L-glutamate preparation, a noodle-added alkaline agent, a preservative preparation, a tar coloring agent, and the like.

The content of the extract of the herbicidal woody plant of the present invention added to foods containing beverages in the process of producing the health functional food of the present invention can be appropriately increased or decreased, By weight to 15% by weight.

The herbaceous plant extracts of the present invention, such as Elaeocarpus sylvestris var. The extract, the Cleyera japonica Thunb. extract, or a combination thereof may be selected from the group consisting of Staphylococcus aureus , Staphylococcus epidermidis , Staphylococcus epidermidis , And Propionibacterium acnes , thereby having an anti-inflammatory effect against various skin inflammation reactions. Accordingly, the descendant water extract, the sour chestnut extract, or a combination thereof can be used safely in pharmaceutical compositions, cosmetic compositions and health functional foods because of no cytotoxicity and skin side effects.

Hereinafter, the present invention will be described in more detail by way of examples. However, the present invention is not limited by the following examples.

Materials and methods

1. Experimental material

Elaeocarpus sylvestris var. Leaves and branches of ellipticus (Thunb.) H. Hara and Cleyera japonica Thunb. were collected from Jeju Island and lyophilized for 48 hours using a freeze dryer (FD8512, Ilsin, Korea) . The dried samples were finely pulverized with a grinder (Hood mixer FM-681C, Hanil, Korea) and stored in a deep freezer (Ultra-low temperature freezer SW-U5-200, Samwon, Korea) at -70 ± 2 ℃ Respectively.

2. Ultrasonic Extraction

Ultrasonography was performed using a 30 × 24 × 14.5 cm ultrasonic cleaner (5510-DTH, Bransonic, USA). 1 g of the dry sample and solvent were mixed in a glass bottle and attached to an acrylic plate. 5 L of water was added to the inside of the ultrasonic bath, and the mixture was filled with water at a height of about 9 cm from the bottom and then extracted for 30 minutes. The water temperature in the ultrasonic bath at the time of extraction was 20 ± 2 ℃, and it was 1.0 ± 0.5 ℃ after 15 minutes, 2.5 ± 0.8 ℃ after 30 minutes and 3.6 ± 0.7 ℃ after 45 minutes. All extracts were extracted once without re-extracting the residues, and extracted twice to add accuracy of extraction. After the extraction, the filtrate was filtered through a vacuum pump (GAST) using a filter paper (Advantec No. 2, Toyo Roshi Kaisha Ltd., Japan), and the final concentration of the filtered extract was determined to be 50 times by using a solvent. After drying for 2 days or more in a dry oven (KPI-507L, Korea Power Ind. Co., Ltd., Korea) at 60 ± 2 ° C in 5 mL of evaporation dish from 2 kinds of extracts obtained from one kind of dry sample, The water content was removed to obtain a soluble solid content (g · g-1 db) of the extract. The remaining extracts were mixed with nitrogen and stored at -70 ° C (SW-UF-200, Samwon Engineering Co., Korea).

3. Strain material

In order to measure the antimicrobial activity of the extracts, the strains used in the experiment were skin-harmful bacteria that could cause skin inflammation, and Staphylococcus aureaus (KCTC 1927), Staphylococcus epidermidis ( KCTC 1917) and Propionibacterium acnes (KCTC 3320) were purchased from the Korean Gene Bank.

4. Culture environment and growth characteristics

(1) Culture environment

The medium used for the microbiological culture was Mueller Hinton (275730, Difco Laboratories, USA) and RCM broth (Reinforced Clostridial Medium, Difco, USA). All microorganisms were cultured at 37 ℃ (Table 1). The microorganisms that were subcultured at intervals of 3 to 5 weeks were inoculated with 100 μL of microbial culture in 10 mL of broth at intervals of 3 to 5 days two weeks before the experiment. P. acnes , an anaerobic bacterium, was cultured in agar broth and placed in a sealed container containing gas pak (AnaeroPack ^® , Mitsubishi Gas Chemical Co., Inc, Japan) and incubated at 37 ° C in a CO ₂ incubator (VS-9108MS, Vision, The strain that was cultured was used.

[Table 1]

^z RCM broth (Reinforced Clostridial), MH broth (Mueller Hinton)

2) Growth curve and number of living cells

Growth curves and microbial counts of the microorganisms used in the experiments were measured to determine the growth characteristics. Preactivated microorganisms were adjusted to an optimal density (OD) of 1.0 at 650 nm using a visible spectrophotometer (Novaspec ^™ Plus, Biochrom, England). 100 μL of broth and OD of 1.0 were added to a 96-well plate (96-cell culture plate; F-type, SPL, Korea) with a Multichannel pipette (Transferpette ^® -8, Brand, Germany) And then the growth curve was measured at intervals of 4 hours while culturing in an incubator at 37 ° C. for 48 hours.

18 hours before the experiment, 10 mL of culture was inoculated into 10 mL of broth and activated, and OD was adjusted to 1.0 at 650 nm immediately before the experiment to measure viable cells. Broth containing 1.5% Agar was dispensed in a volume of 12 mL onto a 90 × 15 mm plate (Petri dish, Green Cross Corp., Korea) and pre-hardened in a clean bench. The culture broth of OD 1.0 was diluted, and 100 μL of the agar broth was divided into 3 replicates for each concentration. After spreading to a spreader (Spreade; 90050, SPL, Korea), colony formed after 24 hours was counted. However, P. acnes The number of viable cells was examined 48 hours after the experiment.

5. Antimicrobial activity of extracts by agar diffusion method

The methanol extract, which was stored at -70 ° C by filling with nitrogen gas, was concentrated under reduced pressure and then dissolved in DMSO to adjust the concentration of soluble solid to 50 mg · mL ^-1 . The extract of 50mg · mL- ¹ concentration was injected 40μL into an 8mm disc (Filter paper, Advantec Toyo Roshi International Inc., Japan) sterilized in a clean bench to be 2mg / disc. After the injection, it was dried to sufficiently volatilize the solvent.

The soft agar broth containing 0.7% agar was sterilized at 121 ° C and 1.2 atm for 15 min, and then the temperature of agar broth was adjusted to 60 ° C in a constant temperature water bath. The pre-activated microbial cultures were inoculated at 1% v / v in soft agar broth at 60 ° C with OD adjusted to 1.0. After inoculating the microbial culture, the mixture was evenly mixed with a magnetic stirrer, and 12 mL of agar broth was dispensed into a 90 × 15 mm plate. The soft agar broth containing microorganisms was blotted on a plate and solidified in a clean bench. The discs were infused with extracts within 15 minutes after dispensing. To facilitate the penetration of the extract into the medium, the cells were incubated in a refrigerator at 4 ° C for 1 hour, transferred to an incubator at 37 ° C for 24 hours, and the clear zone including the diameter of the 8 mm disc was measured after culturing. To compare the antimicrobial activity of the extracts, phenoxyethanol (0H2108, Junsei Chemical Co., Ltd., Japan), methylparaben (8K5015, Junsei Chemical Co., Ltd., Japan) Were diluted to 0.4, 1, 2, and 4 mg / disc and cultured under the same conditions as the extracts.

6. Statistical Analysis

The antimicrobial activity analysis by Agar diffusion method was repeated three times, and the analysis of antimicrobial activity by the concentration of extract and incubation time was repeated 6 times for 1 time twice. The mean and standard errors were calculated using SAS version 9.3 (SAS Instruute Inc., Cary, NC, USA) and statistical analysis was performed using Duncan's multiple range test at a significance level of p <0.05. For the antimicrobial activity according to the extraction conditions, the significance test was performed for each factor between extraction site, solvent and time.

<Examples>

Example 1: Antimicrobial activity against dermatophytes

1-1. Antibacterial activity against Staphylococcus aureaus

The effect of methanol extracts from the paddy field and bamboo shoots on the inhibitory activity of Staphylococcus aureaus (KCTC 1927), known as skin harmful bacteria, was analyzed by agar diffusion method. The extracts obtained by ultrasonication of 1 g of lyophilized and untrimmed leaves and branches with 100% methanol (Merck, Germany) in a solvent for 30 minutes were used in the experiment. The antimicrobial activity at 2 mg / disc concentration was confirmed using an agar diffusion method by adjusting the concentration of soluble solids to a concentration of 50 mg · mL-1, and the results are shown in Table 2 below Respectively. As a control, methylparaben and phenoxyethanol were used as synthetic antibiotics at the concentrations of 0.4, 1.0, 2.0, and 4.0 mg / disc.

[Table 2]

As can be seen from Table 2, methylparaben (13.80mm) and 4mg / disc concentrations of control (4mg / disc) were used as controls for leaves extracts of 2mg / disc and 23.30mm and 2, Of phenoxyethanol (9.80 mm).

1-2. Antibacterial activity against Staphylococcus epidermidis

The effects of methanol extracts of leaves and branches on the moth and elk trees and on the inhibitory activity of Staphylococcus epidermidis ( KCTC 1917), which is known as skin noxious bacteria, were examined in the same manner as above, and the results are shown in Table 3 below.

[Table 3]

As can be seen from Table 3, in the case of leaves extract of 2 mg / disc, 17.60 mm and 2 mg / disc, 19.25 mm, methylparaben (2 mg / disc, 15.20 mm) and phenoxyethanol , 16.30 mm).

1-3. Antibacterial activity against Propionibacterium acnes

Propionibacterium The effect of the methanol extract of the leaves of the pine trees and the tsukkii on the inhibitory activity of acnes (KCTC 3320) was investigated in the same manner as above, and the results are shown in Table 4 below.

[Table 4]

As can be seen from Table 4, in the case of leaves extract of 2 mg / disc, 27.10 mm and 27.2 mm, methylparaben (2 mg / disc, 24.70 mm) and phenoxyethanol (2 mg / disc, , 13.40 mm).

Example 2. Comparison of antibacterial activities according to extraction conditions

2-1. Antibacterial activity according to extraction conditions

The antimicrobial activity of the extracts was determined by using 100% methanol as a solvent and using Staphylococcus aureaus as a skin extract and skin harmful bacteria. (Deionized water, nano pure grade), 80% ethanol (ethanol, Jin chemical parmaceut., Korea) and 100% methanol (methanol, Merck, Germany) were used for the extraction solvent and extraction time. ) As a solvent for 15, 30 and 45 minutes, and the results are shown in Table 5 below. The antimicrobial activity of the extracts stored at -70 ° C was measured at 2mg / disc concentration using agar diffusion method using DMSO as a solvent at a concentration of 50mg · mL-1.

[Table 5]

As can be seen from Table 5, the antimicrobial activity of S. aureus was significantly different depending on the site, and when the methanol extract was applied (23.3 mm) for 30 minutes, Respectively. Methanol (18.6 ~ 23.3mm) and 80% ethanol leaf extract (18.9 ~ 21.8mm) showed no significant difference in S. aureus inhibitory activity.

2-2. Antibacterial activity according to the extraction condition of the sibling tree

The antibacterial activity according to the extraction conditions of the sibuch tree was examined in the same manner as above, and the results are shown in Table 6 below.

[Table 6]

As can be seen from the above Table 6, the S. aureus inhibitory activity of the sibuki bean larvae varied greatly depending on the sites. The 30 minutes methanol extract (21.3 mm) of ultrasound and the 80% ethanol extract (21.3 mm ) Showed the highest inhibitory activity against S. aureus .

Example 3 Comparison of Antimicrobial Activity According to Solvent Fractions

The leaf extracts of 100% methanol were used as a solvent for ultrasonic treatment for 30 minutes to prepare crude extracts. The crude extract was concentrated under reduced pressure using a rotary vacuum evaporator (N-1000, EYELA, Japan) and lyophilized (FD8512, Ilshin Laboratories Ltd., Korea) to obtain 1 g of a solid sample. The obtained soluble solid sample, distilled water and n-hexane were mixed and fractionated at a ratio of 1: 9: 10, and the n-hexane fraction was concentrated to obtain n-hexane fraction. After the fractionation of n-hexane, the aqueous layer was further fractionated with chloroform, ethyl acetate and n-butanol in that order. Each fraction was concentrated under reduced pressure and lyophilized to obtain a soluble fraction. Each fraction was dissolved in distilled water and used for antibacterial experiments. The inhibitory activity against the staphylococcus aureaus of the wash- bottom water according to the solvent fraction was confirmed, and the results are shown in Table 7 below.

[Table 7]

As can be seen from the above Table 7, it was confirmed that the n-butanol fraction exhibits the highest activity among the fractions with a clear zone of 23.1 mm. It was significantly higher than that of crude extract (23.3mm) and higher than that of synthetic antibiotics such as methylparaben and phenoxyethanol. Chloroform fraction (13.5mm) showed significantly higher inhibitory activity than phenoxyethanol (11.9mm).

While the present invention has been particularly shown and described with reference to specific embodiments thereof, those skilled in the art will readily appreciate that many modifications are possible in the exemplary embodiments without materially departing from the novel teachings and advantages of this invention. something to do. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Claims (15)

A pharmaceutical composition for the prevention or treatment of inflammatory skin diseases,
The above-mentioned herbaceous plant extracts were obtained from Elaeocarpus sylvestris var. a leaf extract of ellipticus (Thunb.) H. Hara], a leaf extract of Cleyera japonica Thunb., or a combination thereof is ultrasonically extracted with methanol as a solvent,
The herbicidal herb extract of the present invention is characterized by inhibiting the activity of Staphylococcus aureus , Staphylococcus epidermidis , and Propionibacterium acnes . &Lt; / RTI &gt;
The method according to claim 1,
Wherein the inflammatory skin disease is any one selected from the group consisting of atopic dermatitis, contact dermatitis, seborrhea, and acne.
delete delete The method according to claim 1,
Wherein the herbaceous plant extract is an alcoholic extract of an evergreen tree plant.
1. A cosmetic composition for improving inflammatory skin diseases, comprising an extract of an herbaceous woody plant,
The above-mentioned herbaceous plant extracts were obtained from Elaeocarpus sylvestris var. a leaf extract of ellipticus (Thunb.) H. Hara], a leaf extract of Cleyera japonica Thunb., or a combination thereof is ultrasonically extracted with methanol as a solvent,
The herbicidal herb extract of the present invention is characterized by inhibiting the activity of Staphylococcus aureus , Staphylococcus epidermidis , and Propionibacterium acnes . &Lt; / RTI &gt;
The method of claim 6, wherein
Wherein the inflammatory skin disease is any one selected from the group consisting of atopic dermatitis, contact dermatitis, seborrhea, and acne.
delete delete The method according to claim 6,
The cosmetic composition of the present invention can be used in cosmetics such as soft longevity, astringent lotion, nutritional lotion, nutritional cream, massage cream, essence, eye cream, eye essence, cleansing cream, cleansing foam, cleansing water, pack, powder, body lotion, body cream, Wherein the cosmetic composition is selected from the group consisting of an essence, a makeup base, a foundation, a hair dye, a shampoo, a rinse and a body cleanser.
A health functional food for the improvement of inflammatory skin diseases including an herbaceous plant extract,
The above-mentioned herbaceous plant extracts were obtained from Elaeocarpus sylvestris var. a leaf extract of ellipticus (Thunb.) H. Hara], a leaf extract of Cleyera japonica Thunb., or a combination thereof is ultrasonically extracted with methanol as a solvent,
The herbicidal herb extract of the present invention is characterized by inhibiting the activity of Staphylococcus aureus , Staphylococcus epidermidis , and Propionibacterium acnes . Health functional food, which is one.
The method of claim 11, wherein
Wherein said inflammatory skin disease is any one selected from the group consisting of atopic dermatitis, contact dermatitis, seborrhea, and acne.
delete delete 12. The method of claim 11,
The health functional food may be any one of meat, sausage, bread, chocolate, candy, snack, confectionery, pizza, ramen, gum, ice cream, soup, beverage, tea, functional water, drink, alcoholic beverage, Or a pharmaceutically acceptable salt thereof.