KR102403435B1 - 이중 관형 구조 - Google Patents

이중 관형 구조 Download PDF

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KR102403435B1
KR102403435B1 KR1020187029134A KR20187029134A KR102403435B1 KR 102403435 B1 KR102403435 B1 KR 102403435B1 KR 1020187029134 A KR1020187029134 A KR 1020187029134A KR 20187029134 A KR20187029134 A KR 20187029134A KR 102403435 B1 KR102403435 B1 KR 102403435B1
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cells
epithelial
mesenchymal
microfluidic channel
gel
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KR20180131569A (ko
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폴 부우투
도로타 마우고르자타 쿠렉
아드리아너스 테오도루스 요오레
세바스티안 요하네스 트리치
헨리에테 레오노레 란츠
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미메타스 비.브이.
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0697—Artificial constructs associating cells of different lineages, e.g. tissue equivalents
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M31/00—Means for providing, directing, scattering or concentrating light
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/089—Virtual walls for guiding liquids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/16—Surface properties and coatings
    • B01L2300/161—Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/16—Surface properties and coatings
    • B01L2300/161—Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
    • B01L2300/163—Biocompatibility
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0457—Moving fluids with specific forces or mechanical means specific forces passive flow or gravitation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/13—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells
    • C12N2506/1346—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from mesenchymal stem cells
    • C12N2506/1392—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from mesenchymal stem cells from mesenchymal stem cells from other natural sources
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/23—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from cells of the gastro-intestinal tract
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2513/00—3D culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2531/00—Microcarriers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Clinical Laboratory Science (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Sustainable Development (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Fluid Mechanics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
KR1020187029134A 2016-03-09 2017-03-09 이중 관형 구조 Active KR102403435B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
NL2016404A NL2016404B1 (en) 2016-03-09 2016-03-09 Double tubular structures.
NL2016404 2016-03-09
PCT/NL2017/050145 WO2017155399A1 (en) 2016-03-09 2017-03-09 Double tubular structures

Publications (2)

Publication Number Publication Date
KR20180131569A KR20180131569A (ko) 2018-12-10
KR102403435B1 true KR102403435B1 (ko) 2022-05-30

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US (1) US20190076842A1 (https=)
EP (1) EP3426769B1 (https=)
JP (1) JP7117245B2 (https=)
KR (1) KR102403435B1 (https=)
CN (1) CN109072184A (https=)
AU (1) AU2017231580B2 (https=)
BR (1) BR112018068303A2 (https=)
CA (1) CA3017100A1 (https=)
NL (1) NL2016404B1 (https=)
RU (1) RU2756404C1 (https=)
WO (1) WO2017155399A1 (https=)

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Publication number Priority date Publication date Assignee Title
CA3076664C (en) * 2017-09-21 2023-10-10 EMULATE, Inc. Physiology and pathophysiology of human gut: intestine-on-chip
NL2020518B1 (en) 2018-03-02 2019-09-12 Mimetas B V Device and method for performing electrical measurements
US20210341462A1 (en) 2018-10-05 2021-11-04 The Trustees Of The University Of Pennsylvania Artificial human pulmonary airway and methods of preparation
CN109926112A (zh) * 2019-04-10 2019-06-25 中南大学 三维微结构聚合物微流控芯片的叠层制造方法
NL2024202B1 (en) * 2019-11-08 2021-07-20 Mimetas B V Microfluidic cell culture system
US12480079B2 (en) 2021-04-23 2025-11-25 Massachusetts Institute Of Technology Fluidic platforms for perfusable vascularized tissues with infiltrates
EP4358982A4 (en) * 2021-06-25 2025-05-14 The J. David Gladstone Institutes, A Testamentary Trust Established under The Will of J. David Gladstone Primordial germ cells
CN115301301B (zh) * 2022-07-29 2026-01-13 江苏艾玮得生物科技有限公司 微流控芯片
WO2024072511A1 (en) * 2022-09-30 2024-04-04 Xellar, Inc. Microfluidic devices containing hydrogels, and techniques for making and using
PL443644A1 (pl) * 2023-01-31 2024-08-05 Politechnika Warszawska System mikroprzepływowy do wielonarządowej hodowli komórkowej i jego zastosowanie
WO2025232822A1 (en) * 2024-05-09 2025-11-13 Anivance Ai Corporation Chip, microfluidic cell culture device including the same, and method for cell culture using the same

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20140038279A1 (en) * 2011-02-28 2014-02-06 President And Fellows Of Harvard College Cell culture system

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4829000A (en) 1985-08-30 1989-05-09 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Reconstituted basement membrane complex with biological activity
US9157060B2 (en) * 2005-06-09 2015-10-13 The United States Of America, As Represented By The Secretary Of The Navy Micro blood vessels and tissue ducts
EP1937326B1 (en) * 2005-10-21 2018-09-12 CellResearch Corporation Pte Ltd Isolation and cultivation of stem/progenitor cells from the amniotic membrane of umbilical cord and uses of cells differentiated therefrom
US20110129850A1 (en) 2006-12-22 2011-06-02 UCLA Office of Intellectual Property Microfluidic platform for cell culture and assay
EP2222837A2 (en) * 2007-02-09 2010-09-01 The General Hospital Corporation Methods for the induction of a cell to enter the islet 1+ lineage and a method for the expansion thereof
WO2009154466A1 (en) * 2008-06-20 2009-12-23 Universiteit Twente Self-assembling tissue modules
EP2213364A1 (en) 2009-01-30 2010-08-04 Albert-Ludwigs-Universität Freiburg Phase guide patterns for liquid manipulation
US9752124B2 (en) 2009-02-03 2017-09-05 Koninklijke Nederlandse Akademie Van Wetenschappen Culture medium for epithelial stem cells and organoids comprising the stem cells
TW201137121A (en) * 2010-04-26 2011-11-01 Univ Nat Changhua Education Cell culture real-time observation system
GB201103917D0 (en) 2011-03-08 2011-04-20 Univ Leiden Apparatus for and methods of processing liquids or liquid based substances
CN103087912A (zh) * 2011-10-27 2013-05-08 中国科学院大连化学物理研究所 一种产生稳定浓度梯度的微流控芯片及细胞共培养方法
WO2013085909A1 (en) * 2011-12-05 2013-06-13 Research Triangle Institute Human conducting airway model comprising multiple fluidic pathways
CN106399094A (zh) 2012-04-01 2017-02-15 Emd密理博公司 细胞培养和梯度迁移化验方法和装置
GB2505706A (en) * 2012-09-10 2014-03-12 Univ Leiden Apparatus comprising meniscus alignment barriers
WO2015171074A1 (en) * 2014-05-05 2015-11-12 National University Of Singapore Methods of producing tissue-mimetic constructs and uses thereof
JP6710000B2 (ja) * 2014-05-20 2020-06-17 国立大学法人 東京大学 マイクロファイバ
CA3001342A1 (en) * 2015-10-16 2017-04-20 Wake Forest University Health Sciences Multi-layer airway organoids and methods of making and using the same

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20140038279A1 (en) * 2011-02-28 2014-02-06 President And Fellows Of Harvard College Cell culture system

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
J BIOMED MATER RES., vol.72A, pp.146~160(2005)*

Also Published As

Publication number Publication date
KR20180131569A (ko) 2018-12-10
EP3426769B1 (en) 2024-09-25
EP3426769A1 (en) 2019-01-16
US20190076842A1 (en) 2019-03-14
JP2019507602A (ja) 2019-03-22
WO2017155399A1 (en) 2017-09-14
CN109072184A (zh) 2018-12-21
EP3426769C0 (en) 2024-09-25
BR112018068303A2 (pt) 2019-03-06
AU2017231580A1 (en) 2018-09-27
NL2016404B1 (en) 2017-09-26
NL2016404A (en) 2017-09-19
JP7117245B2 (ja) 2022-08-12
CA3017100A1 (en) 2017-09-14
AU2017231580B2 (en) 2023-03-30
RU2756404C1 (ru) 2021-09-30

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