BR112018068303A2 - método de cultivo e/ou monitoramento de células epiteliais usando um sistema de cultura celular microfluida, uso das células no sistema de cultura celular microfluida, composição ou sistema e sistema de cultura celular microfluida - Google Patents

método de cultivo e/ou monitoramento de células epiteliais usando um sistema de cultura celular microfluida, uso das células no sistema de cultura celular microfluida, composição ou sistema e sistema de cultura celular microfluida

Info

Publication number
BR112018068303A2
BR112018068303A2 BR112018068303A BR112018068303A BR112018068303A2 BR 112018068303 A2 BR112018068303 A2 BR 112018068303A2 BR 112018068303 A BR112018068303 A BR 112018068303A BR 112018068303 A BR112018068303 A BR 112018068303A BR 112018068303 A2 BR112018068303 A2 BR 112018068303A2
Authority
BR
Brazil
Prior art keywords
cell culture
culture system
cells
microfluid cell
microfluid
Prior art date
Application number
BR112018068303A
Other languages
English (en)
Portuguese (pt)
Inventor
Theodorus Joore Adrianus
Malgorzata Kurek Dorota
Leonore Lanz Henriëtte
Vulto Paul
Johannes Trietsch Sebastiaan
Original Assignee
Mimetas B V
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mimetas B V filed Critical Mimetas B V
Publication of BR112018068303A2 publication Critical patent/BR112018068303A2/pt

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M31/00—Means for providing, directing, scattering or concentrating light
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0697—Artificial constructs associating cells of different lineages, e.g. tissue equivalents
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/089—Virtual walls for guiding liquids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/16—Surface properties and coatings
    • B01L2300/161—Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/16—Surface properties and coatings
    • B01L2300/161—Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
    • B01L2300/163—Biocompatibility
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0457—Moving fluids with specific forces or mechanical means specific forces passive flow or gravitation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/13—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells
    • C12N2506/1346—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from mesenchymal stem cells
    • C12N2506/1392—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from mesenchymal stem cells from mesenchymal stem cells from other natural sources
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/23—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from cells of the gastro-intestinal tract
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2513/00—3D culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2531/00—Microcarriers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Clinical Laboratory Science (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Sustainable Development (AREA)
  • Physics & Mathematics (AREA)
  • Fluid Mechanics (AREA)
  • Hematology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
BR112018068303A 2016-03-09 2017-03-09 método de cultivo e/ou monitoramento de células epiteliais usando um sistema de cultura celular microfluida, uso das células no sistema de cultura celular microfluida, composição ou sistema e sistema de cultura celular microfluida BR112018068303A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
NL2016404A NL2016404B1 (en) 2016-03-09 2016-03-09 Double tubular structures.
PCT/NL2017/050145 WO2017155399A1 (en) 2016-03-09 2017-03-09 Double tubular structures

Publications (1)

Publication Number Publication Date
BR112018068303A2 true BR112018068303A2 (pt) 2019-03-06

Family

ID=55967375

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112018068303A BR112018068303A2 (pt) 2016-03-09 2017-03-09 método de cultivo e/ou monitoramento de células epiteliais usando um sistema de cultura celular microfluida, uso das células no sistema de cultura celular microfluida, composição ou sistema e sistema de cultura celular microfluida

Country Status (11)

Country Link
US (1) US20190076842A1 (https=)
EP (1) EP3426769B1 (https=)
JP (1) JP7117245B2 (https=)
KR (1) KR102403435B1 (https=)
CN (1) CN109072184A (https=)
AU (1) AU2017231580B2 (https=)
BR (1) BR112018068303A2 (https=)
CA (1) CA3017100A1 (https=)
NL (1) NL2016404B1 (https=)
RU (1) RU2756404C1 (https=)
WO (1) WO2017155399A1 (https=)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019060735A1 (en) * 2017-09-21 2019-03-28 EMULATE, Inc. PHYSIOLOGY AND PATHOPHYSIOLOGY OF HUMAN INTESTINES: INTESTIN-ON-CHIP
NL2020518B1 (en) 2018-03-02 2019-09-12 Mimetas B V Device and method for performing electrical measurements
EP3861097A4 (en) * 2018-10-05 2022-07-27 The Trustees of the University of Pennsylvania ARTIFICIAL HUMAN PULMONARY TRACHE AND METHOD OF PRODUCTION
CN109926112A (zh) * 2019-04-10 2019-06-25 中南大学 三维微结构聚合物微流控芯片的叠层制造方法
NL2024202B1 (en) * 2019-11-08 2021-07-20 Mimetas B V Microfluidic cell culture system
US12480079B2 (en) 2021-04-23 2025-11-25 Massachusetts Institute Of Technology Fluidic platforms for perfusable vascularized tissues with infiltrates
JP2024522861A (ja) * 2021-06-25 2024-06-21 ザ ジェイ． デビッド グラッドストーン インスティテューツ、 ア テスタメンタリー トラスト エスタブリッシュド アンダー ザ ウィル オブ ジェイ． デビッド グラッドストーン 始原生殖細胞
CN115301301B (zh) * 2022-07-29 2026-01-13 江苏艾玮得生物科技有限公司 微流控芯片
WO2024072513A1 (en) * 2022-09-30 2024-04-04 Xellar, Inc. Pipette interface systems and methods for viscous fluid injection
PL443644A1 (pl) * 2023-01-31 2024-08-05 Politechnika Warszawska System mikroprzepływowy do wielonarządowej hodowli komórkowej i jego zastosowanie
WO2025232822A1 (en) * 2024-05-09 2025-11-13 Anivance Ai Corporation Chip, microfluidic cell culture device including the same, and method for cell culture using the same

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4829000A (en) 1985-08-30 1989-05-09 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Reconstituted basement membrane complex with biological activity
US9157060B2 (en) * 2005-06-09 2015-10-13 The United States Of America, As Represented By The Secretary Of The Navy Micro blood vessels and tissue ducts
CN103555655B (zh) * 2005-10-21 2018-02-27 细胞研究私人有限公司 自脐带羊膜分离和培养干/祖细胞及其分化的细胞的应用
WO2008079320A1 (en) 2006-12-22 2008-07-03 The Regents Of The University Of California Microfluidic platform for cell culture and assay
WO2008098184A2 (en) * 2007-02-09 2008-08-14 The General Hospital Corporation Methods for the induction of a cell to enter the islet 1+ lineage and a method for the expansion thereof
EP2310491B1 (en) * 2008-06-20 2018-03-07 Universiteit Maastricht Self-assembling tissue modules
EP2213364A1 (en) 2009-01-30 2010-08-04 Albert-Ludwigs-Universität Freiburg Phase guide patterns for liquid manipulation
US9752124B2 (en) 2009-02-03 2017-09-05 Koninklijke Nederlandse Akademie Van Wetenschappen Culture medium for epithelial stem cells and organoids comprising the stem cells
TW201137121A (en) * 2010-04-26 2011-11-01 Univ Nat Changhua Education Cell culture real-time observation system
DK2681306T3 (en) * 2011-02-28 2019-04-23 Harvard College CELL CULTURE SYSTEM
GB201103917D0 (en) 2011-03-08 2011-04-20 Univ Leiden Apparatus for and methods of processing liquids or liquid based substances
CN103087912A (zh) * 2011-10-27 2013-05-08 中国科学院大连化学物理研究所 一种产生稳定浓度梯度的微流控芯片及细胞共培养方法
US20140335496A1 (en) * 2011-12-05 2014-11-13 Research Triangle Institute Human conducting airway model comprising multiple fluidic pathways
JP2015517804A (ja) 2012-04-01 2015-06-25 イー・エム・デイー・ミリポア・コーポレイシヨン 細胞培養及び傾斜移動アッセイ方法及び装置
GB2505706A (en) * 2012-09-10 2014-03-12 Univ Leiden Apparatus comprising meniscus alignment barriers
US20170182221A1 (en) * 2014-05-05 2017-06-29 National University Of Singapore Methods Of Producing Tissue-Mimetic Constructs And Uses Thereof
WO2015178427A1 (ja) * 2014-05-20 2015-11-26 国立大学法人 東京大学 中空マイクロファイバ
EP3362554B1 (en) * 2015-10-16 2025-09-03 Wake Forest University Health Sciences Multi-layer airway organoids and methods of making and using the same

Also Published As

Publication number Publication date
JP7117245B2 (ja) 2022-08-12
CA3017100A1 (en) 2017-09-14
CN109072184A (zh) 2018-12-21
JP2019507602A (ja) 2019-03-22
AU2017231580B2 (en) 2023-03-30
RU2756404C1 (ru) 2021-09-30
EP3426769A1 (en) 2019-01-16
EP3426769C0 (en) 2024-09-25
KR102403435B1 (ko) 2022-05-30
KR20180131569A (ko) 2018-12-10
NL2016404B1 (en) 2017-09-26
US20190076842A1 (en) 2019-03-14
EP3426769B1 (en) 2024-09-25
NL2016404A (en) 2017-09-19
WO2017155399A1 (en) 2017-09-14
AU2017231580A1 (en) 2018-09-27

Similar Documents

Publication Publication Date Title
BR112018068303A2 (pt) método de cultivo e/ou monitoramento de células epiteliais usando um sistema de cultura celular microfluida, uso das células no sistema de cultura celular microfluida, composição ou sistema e sistema de cultura celular microfluida
BR112016023769A2 (pt) célula hospedeira recombinante para a expressão de proteínas de interesse
BR112016023304A2 (pt) célula hospedeira recombinante engendrada para superexpressar proteínas auxiliares
CU20180024A7 (es) Moléculas de unión a linfopoyetina estromal tímica (tslp), composiciones farmacéuticas que las contienen y método de producción de las mismas
BR112018002214A2 (pt) ?métodos para cultivar células e para produzir uma proteína, meios de cultura de células cho e de batelada alimentada e/ou alimentação, e, alimentação de cultura de células cho?
PE20181529A1 (es) Vacunas de acido nucleico para el virus varicela-zoster (vzv)
MX2017003847A (es) Proteinas biespecificas activables por proteasas.
AR090017A1 (es) Agonistas de fgfr1 y sus metodos de uso
BR112019000430A2 (pt) sistema crispr-cas para uma célula hospedeira de algas
BR112017017025A2 (pt) métodos e composições para analizar componentes celulares
BR112015022490A2 (pt) métodos e composições para classificação de amostras
EP4368992A3 (en) Simultaneous quantification of a plurality of proteins in a user-defined region of a cross-sectioned tissue
EP4368728A3 (en) Simultaneous quantification of gene expression in a user-defined region of a cross-sectioned tissue
BR112016020287A2 (pt) células resistentes a vírus e usos das mesmas
AR095348A1 (es) Medios de cultivo celular y métodos de producción de anticuerpos
MX2015013202A (es) Cultivo, produccion, procesamiento y uso de cannabis de especialidad.
MX2021015301A (es) Métodos de cultivo celular y composiciones para la producción de anticuerpos.
AR094375A1 (es) Métodos de uso de inhibidores del ciclo celular para modular una o más propiedades de un cultivo celular
MX2016004564A (es) Cultivo celular metabolicamente optimizado.
UY32501A (es) Anticuerpo antagonista específico para el heterodímero alfa4-beta7
MX389093B (es) Composiciones y metodos para la glicosilacion de proteinas.
WO2016154623A3 (en) Anti-cd133 monoclonal antibodies and related compositions and methods
BR112016028512A8 (pt) método de cultivo de uma célula de mamífero e método de produção de uma proteína recombinante
DK3284816T3 (da) Dyrkningsmedietilsætningsstof, dyrkningsmediesammensætning og fremgangsmåde til at dyrke celler eller væv ved anvendelse af samme
CO2017006156A2 (es) Un método para manufacturar una proteina de interes, donde dicha proteina es expresada en una celula huesped

Legal Events

Date Code Title Description
B350 Update of information on the portal [chapter 15.35 patent gazette]
B06W Patent application suspended after preliminary examination (for patents with searches from other patent authorities) chapter 6.23 patent gazette]
B11B Dismissal acc. art. 36, par 1 of ipl - no reply within 90 days to fullfil the necessary requirements