KR102351953B1 - Cosmetic Composition for Moisturizing Skin and Soothing Skin Comprising Extracts of Angelica Archangelica and Forsythia Suspense Fruits - Google Patents

Abstract

The present invention relates to a skin moisturizing cosmetic composition containing angelica extract and yeonkyo extract as active ingredients and having a skin soothing effect.

Description

Cosmetic Composition for Moisturizing Skin and Soothing Skin Comprising Extracts of Angelica Archangelica and Forsythia Suspense Fruits

The present invention relates to a skin moisturizing cosmetic composition containing angelica extract and yeonkyo extract as active ingredients and having a skin soothing effect.

The skin forms a major barrier from the external environment, and plays an important role in protecting our body from wounds, infections, water loss and UV rays. Ultraviolet rays are one of the main causes of inducing skin aging by damaging the skin, and many studies on the relationship between ultraviolet rays and the skin are being conducted in the skin field.

Moisturizing the skin protects the body from various external stimuli and at the same time has a barrier function to block moisture from the inside. will do Structurally, the cells of the stratum corneum act like bricks, and the intercellular lipids of the keratinocytes act as mortar and constitute the skin barrier.

However, as people age, dry skin tends to increase, and the need for a skin moisturizer is increasing due to indoor heating and air pollution. For this purpose, polyhydric alcohols and sugars as skin moisturizers have been mixed and used in cosmetics as general moisturizing agents. However, there is a disadvantage that stickiness occurs on the skin, and there is also a problem that does not give a sufficient moisturizing effect to the skin.

In addition, in summer when humidity is very high, the skin is sticky by drawing moisture from the outside, and in winter when the humidity of the external atmosphere is low, moisture is drawn from the skin and the skin is rather dry. Human skin is very sensitive to relative humidity and usually feels comfortable when the relative humidity is 40-70%. Relative humidity is a value expressed as a percentage of the amount of saturated water vapor at the same temperature as the amount of water vapor in ^{1 m 3 of air.} In the case of Korea, the humidity is high in summer and very humid, and in winter, the relative humidity is low and the air is very dry. Human skin is also greatly affected by seasonal changes and changes in humidity. Discomfort increases in summer when the relative humidity is high due to high temperature and humidity, and when the relative humidity is low in dry winter, the lips and skin also become dry and cracked, sometimes causing difficulty in breathing. I also feel When the relative humidity is high, it absorbs moisture from the air and becomes sticky. On the contrary, when the relative humidity is low, the moisture in the skin evaporates and becomes dry. In particular, when the relative humidity is low, the dryness of the skin is often accompanied by tightness and itching.

Various cosmetic compositions use various moisturizing agents such as oils, sugars, and polyols to overcome the dryness of the skin. For example, it uses ceramide, cholesterol, and fatty acids, which are components of the skin, to strengthen the skin barrier function, thereby suppressing moisture evaporating from the skin, or using sugars and polyols, which have strong ability to bind moisture on the skin surface, to retain moisture in the skin. to enhance the moisturizing effect of the skin. However, it is difficult to maintain the effect for a long time in winter when the relative humidity is low, and it is rather unpleasant in the summer when the relative humidity is high, making it difficult to prepare an appropriate cosmetic composition according to the change in relative humidity.

Various patent documents have been published on the moisturizing effect of plant extracts. Korean Patent No. 10-1814498 discloses a cosmetic composition containing a mixed extract of eucalyptus leaves and red shamrock leaves to have skin protection effects such as skin moisturizing enhancement and skin irritation relief. In addition, Korean Patent Registration No. 10-1926263 discloses a cosmetic composition containing marjoram, molochia and lemon balm mixed fermented extract to improve skin wrinkles, skin whitening, skin moisturizing, skin irritation relief and antioxidant effects.

Accordingly, the present inventors have repeatedly studied to prepare a cosmetic composition that maintains the moisturizing power of the skin and maintains the effect for a long time regardless of seasonal changes and changes in relative humidity, as a result, angelica extract and yeonkyo extract When applied to the skin, it was confirmed that a continuous moisturizing effect can be exhibited regardless of changes in relative humidity, and the present invention was completed. It is an object of the present invention to provide a cosmetic composition for moisturizing the skin that imparts continuous moisturizing power regardless of changes in relative humidity and has a skin soothing effect through this.

The above object is achieved by a cosmetic composition for moisturizing the skin comprising an angelica extract and yeonkyo extract as active ingredients.

Preferably, the angelica extract may be contained in an amount of 0.0001 to 80.0% by weight based on the total weight of the composition.

Also preferably, the yeonkyo extract may be contained in an amount of 0.0001 to 10.0% by weight based on the total weight of the composition.

The cosmetic composition has a skin soothing effect.

The cosmetic composition according to the present invention contains angelica extract and yeonkyo extract as active ingredients, and has an excellent moisturizing effect and skin soothing effect.

1 is a graph showing the amount of transepidermal moisture loss of a cosmetic composition according to the present invention.

All technical terms used in the present invention, unless otherwise defined, have the following definitions and have the meanings as commonly understood by one of ordinary skill in the art of the present invention. In addition, although preferred methods and samples are described herein, similar or equivalent ones are also included in the scope of the present invention.

The term "about" means 30, 25, 20, 15, 10, 9, 8, 7, 6, 5, means an amount, level, value, number, frequency, percentage, dimension, size, amount, weight or length varying by 4, 3, 2 or 1%.

Throughout this specification, unless the context requires otherwise, the terms "comprises" and "comprising" include, but are not limited to, a given step or element, or group of steps or elements, but any other step or element, or It is to be understood that a step or group of elements is not excluded.

The present invention relates to a cosmetic composition for moisturizing the skin comprising an angelica extract and yeonkyo extract as active ingredients.

Angelica extract and Yeongyo extract according to the present invention do not show cytotoxicity and can exhibit moisturizing effect.

Angelica (Angelica archangelica) is a plant of the umbel family native to Europe, and it is derived from the word 'angelus' meaning 'angel'. became The aromatic bitter taste of angelica is very good for the stomach and is known to relieve indigestion, abdominal pain, colic, flatulence, and has a stimulating and warming effect on the digestive system. In addition, angelica promotes blood circulation and is effective in anemia, and has been confirmed to have antibacterial and antifungal actions.

Yeongyo refers to the fruit of forsythia, a plant in the ash family. Yeongyo is a deciduous shrub with a height of 2~3m, and its branches are long and thin, hanging down or hanging down and hollow. The leaf has 1 or 3 bifoliate opposite and is egg-shaped, and the side bottom is wide wedge-shaped. The flowers bloom before the leaves, and 1-4 clusters are epiphytic on the leaves. The fruit is narrow oval, with a short beak at the tip, and splits into 2 pieces when fully ripe. It grows in the wasteland of the mountains and is harvested when the fruits are first or fully ripe, and dried in the sun.

Angelica extract and Yeongyo extract have been studied to some extent on physiology, nutrition, or activity, but research on their skin moisturizing effect is insufficient.

The cosmetic composition of the present invention contains an angelica extract and yeonkyo extract sufficient to maintain skin moisture homeostasis. The content of the angelica extract is 0.0001 to 80.0% by weight, preferably 0.001 to 50.0% by weight, based on the total weight of the cosmetic composition, and the yeonkyo extract is preferably 0.0001 to 10.0% by weight based on the total weight.

When the content of angelica extract is less than 0.0001% by weight based on the total weight of the cosmetic composition, there is a problem that the skin moisturizing effect is somewhat insufficient, and when the content of angelica extract exceeds 80% by weight based on the total weight of the cosmetic composition, the content increases. Accordingly, there is a problem in that the stability of the formulation and skin sensitivity increase.

When the content of yeonkyo extract is less than 0.0001 wt% based on the total weight of the cosmetic composition, there is a problem that the skin moisturizing effect is somewhat insufficient. There is a problem with

According to one embodiment of the present invention, angelica extract and Yeongyo extract may be included in a mixture in a weight ratio of 2:1 to 100:1. More preferably, the angelica extract and Yeongyo extract may be mixed in a weight ratio of 5:1.

The angelica extract of the present invention can be prepared according to a conventional method of cooling extraction using liquid nitrogen known in the art, that is, under conditions of conventional temperature and pressure, using a conventional solvent. Preferably water, anhydrous or hydrous lower alcohols having 1 to 4 carbon atoms (e.g., methanol, ethanol, propanol and butanol), propylene glycol, 1,3-butylene glycol, glycerin, acetone, diethyl ether, ethyl acetate , butyl acetate, dichloromethane, chloroform, hexane, and can be obtained using one or more extraction solvents selected from the group consisting of mixtures thereof. More preferably, it is obtained using ethanol, 70% (v/v) ethanol or water, and most preferably, it can be obtained using 70% (v/v) ethanol.

The components contained in the cosmetic composition of the present invention include components commonly used in cosmetic compositions other than angelica extract, for example, conventional auxiliary agents such as antioxidants, stabilizers, solubilizers, vitamins, pigments and fragrances, and carriers. include

The cosmetic composition of the present invention may be prepared in any formulation conventionally prepared in the art, for example, a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, cleansing containing surfactant, etc. It may be formulated as, but is not limited thereto. More specifically, it is formulated in the form of flexible lotion, nourishing lotion, milk lotion, nourishing cream, massage cream, essence, eye cream, cleansing lotion, cleansing cream, cleansing foam, cleansing water, powder wash, cleansing oil, mask and pack. can be manufactured.

When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, ester oil, wax, paraffin, starch, tracanth, cellulose derivative, carbomer, polyacrylamide, polyethylene glycol, silicone, bentonite, Silica, talc or zinc oxide may be used.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 ,3-butylene glycol, oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan.

When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester; Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar, or tracanth may be used.

When the formulation of the present invention is a surfactant-containing cleansing agent, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide ether as carrier components Sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester may be used.

The cosmetic composition containing the angelica extract and yeonkyo extract of the present invention maintains skin moisture homeostasis, and has excellent skin safety because irritation to the skin is low.

Hereinafter, the present invention will be described in more detail through examples. These Examples are for explaining the present invention in more detail, and the scope of the present invention is not limited by these Examples. In addition, parts, %, and ratios used in this example correspond to weight standards unless otherwise specified.

production example 1: Preparation of Angelica Extract

Angelica leaves were washed with clean water and then dried, quenched using liquid nitrogen and crushed, extracted under reflux 3 times for 5 hours each with 70% (V/V) aqueous ethanol solution, cooled and cooled, Whatman (Whatman) ) was filtered with #4 filter paper. The filtered extract was concentrated under reduced pressure at 50° C. or lower and freeze-dried.

production example 2: annual school extract preparation

After washing the dried fruit part of forsythia with clean water, drying and powdering, adding a 70% (V/V) aqueous solution of ethanol 10 times the dry weight of the forsythia, and immersing it at room temperature for 5 days to make the active ingredient to extract The extract solution thus extracted was concentrated under reduced pressure using a distillation apparatus equipped with a cooling condenser to obtain an extract of Yeongyo.

Test Example 1: Cytotoxicity test

In order to examine the cytotoxicity of the angelica extract and Yeongyo extract prepared in Preparation Examples 1 and 2, toxicity was confirmed through MTT analysis. MTT (3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyl-tetrazolium bromide (3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyl-tetrazolium The bromide assay is one of the methods to check the growth of cells. The MTT assay utilizes the mitochondrial ability to reduce the water-soluble yellow tetrazolium salt MTT to blue-violet, water-insoluble formazan crystals in living cells. When the cell viability is more than 95%, it is stable to cells, so it is an appropriate method to check the appropriate concentration. Keratinocytes ^{are inoculated in a 48-well plate at 1Х 10 5} cells/well per well, and 1.0 ml of DMEM (Dulbecco Minimum Essential Medium) medium) for 24 hours, each well was treated with 1.0% angelica extract and sodium guaiazulene, and cultured for another 24 hours.The cultured plate was incubated with 0.5 mg/ml MTT solution (Sigma, USA). ) was added, the medium was removed 4 hours later, the cells were lysed with 1.0 ml of DMSO solution, and the absorbance at 570 nm was measured for the reaction solution using an ELISA Reader (Molecular Device, USA). The cell proliferation rate was calculated according to Equation 1 below using the untreated group (control group) and each of the Angelica extract and Yeongyo extract, which were not treated with the extract and Yeongyo extract, as a comparison group, and the results are shown in Table 1 below.

division addition amount Cell proliferation rate (%) control additive-free 100.0 # One Yeongyo extract (0.1%) 101.2 # 2 Yeongyo extract (2.0%) 101.7 #3 Angelica Extract (1.0%) 101.3 # 4 Angelica Extract (2.0%) 102.4 #5 Angelica Extract (3.0%) 102.3 #6 Angelica Extract (5.0%) 103.8 #7 Angelica Extract (10.0%) 102.2 # 8 Angelica Extract (20.0%) 103.2 # 9 Angelica Extract (30.0%) 102.3 # 10 Angelica Extract (80.0%) 104.2

As can be seen from the results in Table 1, since each of the angelica extract and Yeongyo extract of the present invention does not show cytotoxicity to keratinocytes, it was confirmed that the angelica extract and Yeongyo extract of the present invention were significantly safe against cytotoxicity. .

Test Example 2: Measurement of moisturizing power according to concentration

To 20 adult men and women who complain of dryness or who have dry skin, the cosmetic compositions (unit: weight %) of Comparative Examples 1 and 1 to 10 having the composition in Table 2 below were applied to the inner side of the forearm twice daily for 4 weeks made to do Moisturizing power was compared by measuring Trans Epidermal Water Loss (TEWL) under constant temperature and constant humidity conditions (at 24°C and 40% relative humidity) at 2 weeks and 4 weeks after application start and 2 weeks after application. is shown in FIG. 1 .

Raw material name comparative example One Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Example 7 Example 8 Example 9 Example 10 Example 11 Purified water remaining amount remaining amount remaining amount remaining amount remaining amount remaining amount remaining amount remaining amount remaining amount remaining amount remaining amount remaining amount glycerin 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 Dipotacium Glycyrinate 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 Disodium EDIT 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 Hydroxyethyl Cellulose 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 dipropylene glycol 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 antiseptic appropriate amount appropriate amount appropriate amount appropriate amount appropriate amount appropriate amount appropriate amount appropriate amount appropriate amount appropriate amount appropriate amount appropriate amount squalane 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 Polyoxyethylene Hydroxycastor Oil 1.1 1.1 1.1 1.1 1.1 1.1 1.1 1.1 1.1 1.1 1.1 1.1 Sodium P.A. 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Glyceryl Stearate 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Polyglyceryl-3-methylglucose distearate 1.2 1.2 1.2 1.2 1.2 1.2 1.2 1.2 1.2 1.2 1.2 1.2 Spices appropriate amount appropriate amount appropriate amount appropriate amount appropriate amount appropriate amount appropriate amount appropriate amount appropriate amount appropriate amount appropriate amount appropriate amount pigment a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount Angelica Extract - - - 1.0 5.0 10.0 1.0 5.0 10.0 2.0 5.0 10.0 Yeongyo extract - 0.1 2.0 - - - 0.1 0.1 0.1 1.0 2.0 2.0 Sum 100.0 100.0 100.0 100.0 100.0 100.0 100.0 100.0 100.0 100.0 100.0 100.0

<Method for measuring Trans Epidermal Water Loss (TEWL)>

Principle: The amount of water evaporated from the skin is calculated according to the area and time, and the amount of evaporated water is read with a temperature and moisturizing electronic sensor and quantified to measure the moisturizing power of the skin.

1. Environmental conditions: The temperature and humidity are kept constant, leaving only a minimum number of people other than the measurer and the subject (preferably cut off the air flow).

2. Place the TEWL probe on the subject's measurement site.

3. Do not apply excessive force to the measurement area (not to leave marks on the skin) and place the probe lightly on it to form a 90 degree angle with the ground.

4. Measure the value at which the TEWL level of the skin becomes constant over time (usually it takes 1-3 minutes).

5. Be careful not to transmit the body temperature of the person who measures the probe by holding the handle of the probe as far away as possible.

6. Calculate the amount of water evaporated per hour per area (g/m ² /h).

Referring to FIG. 1 , the cosmetic compositions of Comparative Example 1 without extract and Examples 1 to 5 containing only angelica extract or yeonkyo extract did not show specific efficacy/effect in terms of water evaporation, but angelica It was confirmed that an excellent moisturizing effect was observed when the extract and Yeongyo extract were mixed and used for 4 weeks.

Experimental Example 3: Inhibition of IL-1α secretion in HaCaT keratinocytes

HaCaT keratinocytes (German Cancer Research Institute, Germany) were inoculated with DMEM (Dulbeco's Modified Eagle's Medium, Gibco) supplemented with 10% FBS at a density of 2Х ^{10 5 cells/well in a 24-well plate, and humidified at 37°C} 5% CO ₂ Cultured in an incubator for 1 day. After exchanging with serum-free DMEM, all samples of Comparative Example 1 and Examples 1 to 11 were treated at 100 ppm, cultured for 1 day, treated with SDS (sodium dodecyl sulfate, Sigma), and further cultured for 4 hours. . After 4 hours, a portion of the culture medium was taken and used for IL-1α quantification and protein quantification. IL-1α quantification was performed according to the kit's IL-1α quantification method using Endogen's human IL-1α kit. The amount of IL-1α secretion per protein was measured. As a positive control, ketoprofen, which has been previously used as a skin irritation alleviation material, was used, and the final IL-1α production rate (%) was obtained by Equation 1 below. The results are shown in Table 3.

sample IL-1α production rate (%) SDS only processing 100.00 Example 1 91.93 ± 5.40 Example 2 91.50 ± 4.80 Example 3 88.22 ± 5.10 Example 4 86.32 ± 3.60 Example 5 84.28 ± 3.66 Example 6 81.33 ± 3.66 Example 7 81. 22 ± 3.30 Example 8 79.30 ± 3.06 Example 9 75.22 ± 3.30 Example 10 71.15 ± 7.98 Example 11 63.15 ± 7.87 ketoprofen 59.15 ± 7.68

In this experiment, the biosynthesis of IL-1α, which can cause erythema that can occur on the skin through SDS treatment, was observed. After calculating the amount of -1α produced, this is a percentage. Referring to Table 3, it can be seen that the amount of IL-1α secretion from keratinocytes after the SDS treatment of the cosmetic composition containing both the angelica extract and the Yeongyo extract decreased in a concentration-dependent manner compared to the samples containing each extract alone. That is, when it is included in both the Angelica extract and Yeongyo extract, it can be said that the effect of alleviating troubles such as erythema that may occur on the skin by reducing the production amount of IL-1α is great.

Claims (4)

A cosmetic composition for skin moisturizing, comprising a mixture of angelica extract and yeonkyo extract as an active ingredient, wherein the angelica extract and yeonkyo extract are mixed in a weight ratio of 5:2, and have a skin soothing effect. The cosmetic composition for moisturizing the skin according to claim 1, wherein the angelica extract is contained in an amount of 0.0001 to 80.0% by weight based on the total weight of the composition. The cosmetic composition for moisturizing the skin according to claim 1, wherein the yeonkyo extract is contained in an amount of 0.0001 to 10.0 wt% based on the total weight of the composition. delete

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