KR102297271B1 - 당뇨 및 고지혈증을 포함하는 대사성 질환의 예방 또는 치료 활성을 갖는 락토바실러스 람노서스 nbm 17-4 균주 - Google Patents
당뇨 및 고지혈증을 포함하는 대사성 질환의 예방 또는 치료 활성을 갖는 락토바실러스 람노서스 nbm 17-4 균주 Download PDFInfo
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Abstract
Description
도 2는 저 글루코스 DMEM으로 배양한 세포 및 고 글루코스 DMEM으로 배양한 세포에 대해 측정한 TEER를 비교한 결과를 나타낸다.
도 3a는 라이브 셀(프로바이오틱스)을 처리한 세포에서의 TEER 변화값을 측정한 결과를 나타낸다.
도 3b는 CFS (cell free supernatant)를 처리한 세포에서의 TEER 변화값을 측정한 결과를 나타낸다.
도 3c는 CFE (cell free extract)를 처리한 세포에서의 TEER 변화값을 측정한 결과를 나타낸다.
도 4a는 열에 의해 치사된 셀(프로바이오틱스)의 세포(Caco-2) 독성을 확인한 결과를 나타낸다.
도 4b는 CFS의 세포(Caco-2) 독성을 확인한 결과를 나타낸다.
도 4c는 CFE의 세포(Caco-2) 독성을 확인한 결과를 나타낸다.
도 5는 라이브 셀(프로바이오틱스)을 처리한 세포에서의 글루코스 흡수 능력을 평가한 결과를 나타낸다.
도 6a는 라이브 셀(프로바이오틱스)을 처리한 세포에서의 GLUT2 유전자 발현을 측정한 결과를 나타낸다.
도 6b는 라이브 셀(프로바이오틱스)을 처리한 세포에서의 SGLT1 유전자 발현을 측정한 결과를 나타낸다.
도 7은 CFS 또는 CFE의 세포(C2C12) 독성을 확인한 결과를 나타낸다.
도 8은 C2C12 세포에서 인슐린 저항성 세포 모델의 구축하기 위해, 팔미테이트 처리에 따른 phospho-AKT 발현량을 확인한 결과를 나타낸다.
도 9a는 C2C12 세포 인슐린저항성 모델을 사용하여 측정한 프로바이오틱스의 항 당뇨 및 인슐린 저항성 개선능을 웨스턴 블롯으로 평가한 결과를 나타낸다.
도 9b는 도 9a의 웨스턴 블롯 결과에서 pAKT/AKT 비율을 정량화한 결과를 나타낸다.
도 10은 프로바이오틱스의 당뇨병에 대한 효능 평가를 위한 동물 실험의 수행 방법에 대해 나타낸다.
도 11a는 NBM 17-4(프로바이오틱스 균주)의 당뇨병에 대한 효과를 평가하기 위해 마우스의 체중을 측정한 결과를 나타낸다.
도 11b는 NBM 17-4의 당뇨병에 대한 효과를 평가하기 위해 마우스의 식이섭취량을 측정한 결과를 나타낸다.
도 11c는 NBM 17-4의 당뇨병에 대한 효과를 평가하기 위해 마우스의 공복혈당을 측정한 결과를 나타낸다.
도 12는 NBM 17-4의 당뇨병에 대한 효과를 평가하기 위해 마우스를 12시간 공복시킨 후 글루코스로 자극한 뒤의 혈당을 측정한 결과를 나타낸다.
도 13a는 NBM 17-4의 당뇨병에 대한 효과를 평가하기 위해 마우스의 혈장 글루코스를 측정한 결과를 나타낸다.
도 13b는 NBM 17-4의 당뇨병에 대한 효과를 평가하기 위해 마우스의 혈장 인슐린을 측정한 결과를 나타낸다.
도 13c는 NBM 17-4의 당뇨병에 대한 효과를 평가하기 위해 마우스의 HOMA-IR (Homeostatic Model Assessment of Insulin Resistance)을 측정한 결과를 나타낸다.
도 13d는 NBM 17-4의 당뇨병에 대한 효과를 평가하기 위해 마우스의 혈장 C-펩타이드를 측정한 결과를 나타낸다.
도 13e는 NBM 17-4의 당뇨병에 대한 효과를 평가하기 위해 마우스의 혈장 GLP-1 (glucagon-like peptide-1)을 측정한 결과를 나타낸다.
도 14a는 NBM 17-4의 당뇨병에 대한 효과를 평가하기 위해 마우스의 총 트리글리세라이드를 측정한 결과를 나타낸다.
도 14b는 NBM 17-4의 당뇨병에 대한 효과를 평가하기 위해 실험 동물의 총 콜레스테롤을 측정한 결과를 나타낸다.
도 14c는 NBM 17-4의 당뇨병에 대한 효과를 평가하기 위해 마우스의 GOT (Glutamic oxaloacetic transminase)를 측정한 결과를 나타낸다.
도 15a는 NBM 17-4의 당뇨병에 대한 효과를 평가하기 위해 간에서의 IRS2(인슐린 신호전달 관련 유전자)를 측정한 결과를 나타낸다.
도 15b는 NBM 17-4의 당뇨병에 대한 효과를 평가하기 위해 간에서의 AMPK(인슐린 신호전달 관련 유전자)를 측정한 결과를 나타낸다.
도 16a는 NBM 17-4의 당뇨병에 대한 효과를 평가하기 위해 부고환 지방에서 IRS2를 측정한 결과를 나타낸다.
도 16b는 NBM 17-4의 당뇨병에 대한 효과를 평가하기 위해 부고환 지방에서 AMPK를 측정한 결과를 나타낸다.
도 16c는 NBM 17-4의 당뇨병에 대한 효과를 평가하기 위해 부고환 지방에서 Akt2(인슐린 신호전달 관련 유전자)를 측정한 결과를 나타낸다.
도 17a는 NBM 17-4의 당뇨병에 대한 효과를 평가하기 위해 간에서 FOXO1(글루코스 신생 관련 유전자)를 측정한 결과를 나타낸다.
도 17b는 NBM 17-4의 당뇨병에 대한 효과를 평가하기 위해 간에서 G6P(글루코스 신생 관련 유전자)를 측정한 결과를 나타낸다.
도 18은 NBM 17-4의 당뇨병에 대한 효과를 평가하기 위해 간에서 IL-6(염증 사이토카인 관련 유전자)를 측정한 결과를 나타낸다.
도 19a는 NBM 17-4의 당뇨병에 대한 효과를 평가하기 위해 부고환 지방에서 IL-6를 측정한 결과를 나타낸다.
도 19b는 NBM 17-4의 당뇨병에 대한 효과를 평가하기 위해 부고환 지방에서 TNF-α(염증 사이토카인 관련 유전자)를 측정한 결과를 나타낸다.
도 19c는 NBM 17-4의 당뇨병에 대한 효과를 평가하기 위해 부고환 지방에서 MCP-1(염증 사이토카인 관련 유전자)을 측정한 결과를 나타낸다.
도 20a는 NBM 17-4의 당뇨병에 대한 효과를 평가하기 위해 부고환 지방에서 렙틴(식욕 조절 호르몬 관련 유전자)을 측정한 결과를 나타낸다.
도 20b는 NBM 17-4의 당뇨병에 대한 효과를 평가하기 위해 부고환 지방에서 아디포넥틴(식욕 조절 호르몬 관련 유전자)을 측정한 결과를 나타낸다.
도 21a는 NBM 17-4의 당뇨병에 대한 효과를 평가하기 위해 부고환 지방에서 PPARγ(지방 대사 관련 유전자)을 측정한 결과를 나타낸다.
도 21b는 NBM 17-4의 당뇨병에 대한 효과를 평가하기 위해 부고환 지방에서 CD36(지방 대사 관련 유전자)을 측정한 결과를 나타낸다.
도 21c는 NBM 17-4의 당뇨병에 대한 효과를 평가하기 위해 부고환 지방에서 FABP4(지방 대사 관련 유전자)을 측정한 결과를 나타낸다.
도 22a는 NBM 17-4의 당뇨병에 대한 효과를 평가하기 위해 부고환 지방에서 PGC1α(미토콘드리아 신생 관련 유전자)을 측정한 결과를 나타낸다.
도 22b는 NBM 17-4의 당뇨병에 대한 효과를 평가하기 위해 부고환 지방에서 UCP1(미토콘드리아 신생 관련 유전자)을 측정한 결과를 나타낸다.
연번 | 균주 | 종 |
1 | NBM 7-1 | Bifidobacterium longum |
2 | NMC 5-3 | Bifidobacterium pseudocatenulatum |
3 | NMC 3-4 | Lactobacillus acidophilus |
4 | NM 5-3 | Lactobacillus fermentum |
5 | NMC 15-1 | Lactobacillus plantarum |
6 | NBM 17-4 | Lactobacillus rhamnosus |
7 | NM 20-2 | Lactobacillus sakei |
8 | NBM 5-5 | Lactobacillus salivarius |
9 | NM 11-5 | Lactococcus lactis |
10 | NT 13-2 | Lactococcus lactis subsp. lactis |
11 | NM 11-3 | Pediococcus pentosaceus |
유전자 | 프라이머 | 서열 | 서열번호 |
GAPDH | Forward | 5'-CAAGTTCAACGGCACAGTCAAGG-3' | 2 |
Reverse | 5'-ACATACTCAGCACCAGCATCACC-3' | 3 | |
PPARγ | Forward | 5'-CCTGAACATCGAGTGTCGAATAT-3' | 4 |
Reverse | 5'-GGTCTTCTTCTGAATCTTGCAGCT-3' | 5 | |
PGC1α | Forward | 5'-AAGTGTGGAACTCTCTGGAACTG-3' | 6 |
Reverse | 5'-GGGTTATCTTGGTTGGCTTTATG-3' | 7 | |
SREBPF1c | Forward | 5'-TAGTCCGAAGCCGGGTGGGCCGCGCCAT-3' | 8 |
Reverse | 5'-GATGTCGTTCAAAACCGCTGTGTGTCCAGTTC-3' | 9 | |
ACC | Forward | 5'-GCCTCTTCCTGACAAACGAG-3' | 10 |
Reverse | 5'-TGACTGCCGAAACATCTCTG-3' | 11 | |
FAS | Forward | 5'-GCTGCGGAAACATCTCTG-3' | 12 |
Reverse | 5'-GACTCGCTCTCAGATGCCCTAC-3' | 13 | |
CD36 | Forward | 5'-TGGTGGATGGTTTCCTAGCCTTTC-3' | 14 |
Reverse | 5'-TCGCCAACTCCCAGGTACAATC-3' | 15 | |
CPT1 | Forward | 5'-ATCTGGATGGCTATGGTCAAGGTC-3' | 16 |
Reverse | 5'-GTGCTGTCATGCGTTGGAAGTC-3' | 17 |
유전자 | 프라이머 | 서열 | 서열번호 |
UCP1 | Forward | 5'-AGATCTTCTCAGCCGGAGTTT-3' | 18 |
Reverse | 5'-CTGTACAGTTTCGGCAATCCT-3' | 19 | |
UCP2 | Forward | 5'-ACCAAGGGCTCAGAGCATGCA-3;' | 20 |
Reverse | 5'-TGGCTTTCAGGAGAGTATCTTG-3' | 21 | |
PPARα | Forward | 5'-CCTGAACATCGAGTGTCGAGCA-3' | 22 |
Reverse | 5'-GGTCTTCTTCTGAATCTTGCAGCT-3' | 23 | |
Leptin | Forward | 5'-CTCCAAGGTTGTCCAGGGTT-3' | 24 |
Reverse | 5'-AAAACTCCCCACAGAATGGG-3' | 25 | |
Adiponectin | Forward | 5'-GCACTGGCAAGTTCTACTGCAA-3' | 26 |
Reverse | 5'-GTAGGTGAAGAGAACGGCCTTGT-3' | 27 | |
IRS2 | Forward | 5'-TCTACACCCGAGACGAACACT-3' | 28 |
Reverse | 5'-TGGGCCTTTGCCCGATTATG -3' | 29 | |
AMPK | Forward | 5'-AAGATCGGACATACGTCCTG-3' | 30 |
Reverse | 5'- TGCCACTTTATGGCCTGTCAA -3' | 31 | |
AKT2 | Forward | 5'-ACGTGGTGAATACATCAAGACC -3' | 32 |
Reverse | 5'-ACCCAATGAAAGATCCATCACTC-3' | 33 |
유전자 | 프라이머 | 서열 | 서열번호 |
FOXO1 | Forward | 5'-CCTGAGCCTGCTGGAGGAGAG -3' | 34 |
Reverse | 5'-GCACGCTCTTCACCATCCACTC-3' | 35 | |
G6PC | Forward | 5'-AGGTCGTGGCTGGAGTCTTGTC-3' | 36 |
Reverse | 5'-AATGCAGGCGAAGCGGAATGG -3' | 37 | |
TNF-α | Forward | 5'-CCCAGACCCTCACACTCAGATC-3' | 38 |
Reverse | 5'-GCCACTCCAGCTGCTCCTC-3' | 39 | |
IL-6 | Forward | 5'-GAGGATACCACTCCCAACAGACC-3' | 40 |
Reverse | 5'-AAGTGCATCATCGTTGTTCATACA -3' | 41 | |
MCP-1 | Forward | 5'-CCAACTCTCACTGAAGCCAGCTC-3' | 42 |
Reverse | 5'-TTGGGATCATCTTGCTGGTGAA-3' | 43 | |
FABP4 | Forward | 5'-TGGGAACCTGGAAGCTTGTCTC-3' | 44 |
Reverse | 5'-GAATTCCACGCCCAGTTTGA-3' | 45 | |
IL-1β | Forward | 5'-CTGTGTCTTTCCCGTGGACC-3' | 46 |
Reverse | 5'-CAGCTCATATGGGTCCGACA-3' | 47 |
Claims (8)
- 수탁번호 KCTC 14324BP인 락토바실러스 람노서스(Lactobacillus rhamnosus) NBM 17-4 균주에 있어서, 상기 균주는 장벽 강화 효과가 있는 것인, 균주.
- 삭제
- 수탁번호 KCTC 14324BP인 락토바실러스 람노서스(Lactobacillus rhamnosus) NBM 17-4 균주, 상기 균주의 배양물, 상기 배양물의 농축물, 상기 배양물의 건조물 및 이들의 조합으로 이루어진 군으로부터 선택되는 어느 하나 이상을 포함하는 대사성 질환의 예방 또는 치료용 약제학적 조성물로, 상기 균주는 장벽 강화 효과가 있는 것인, 약제학적 조성물.
- 제3항에 있어서, 상기 대사성 질환은 고지혈증, 당뇨병, 고콜레스테롤혈증, 및 고인슐린혈증으로 이루어진 군으로부터 선택되는 것인, 약제학적 조성물.
- 제4항에 있어서, 상기 당뇨병은 제2형 당뇨병인 것인, 약제학적 조성물.
- 수탁번호 KCTC 14324BP인 락토바실러스 람노서스(Lactobacillus rhamnosus) NBM 17-4 균주, 상기 균주의 배양물, 상기 배양물의 농축물, 상기 배양물의 건조물 및 이들의 조합으로 이루어진 군으로부터 선택되는 어느 하나 이상을 포함하는 대사성 질환의 예방 또는 개선용 식품 조성물로, 상기 균주는 장벽 강화 효과가 있는 것인, 식품 조성물.
- 제6항에 있어서, 상기 대사성 질환은 고지혈증, 당뇨병, 고콜레스테롤혈증, 및 고인슐린혈증으로 이루어진 군으로부터 선택되는 것인, 식품 조성물.
- 제7항에 있어서, 상기 당뇨병은 제2형 당뇨병인 것인, 식품 조성물.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1020200165174A KR102297271B1 (ko) | 2020-11-30 | 2020-11-30 | 당뇨 및 고지혈증을 포함하는 대사성 질환의 예방 또는 치료 활성을 갖는 락토바실러스 람노서스 nbm 17-4 균주 |
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Cited By (5)
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US20230131140A1 (en) * | 2021-10-26 | 2023-04-27 | University Of South Florida | Human origin probiotic lactobacilius rhamnosus hl-200 to reduce leaky gut by metabolizing ethanolamine |
KR20230062143A (ko) | 2021-10-29 | 2023-05-09 | 연세대학교 원주산학협력단 | 락토바실러스 람노서스 q1 유효성분으로 함유하는 탈모 예방 또는 치료용 조성물 |
KR20230061882A (ko) | 2021-10-29 | 2023-05-09 | 서울우유협동조합 | 락토바실러스 람노서스 4B15(Lacticaseibacillus rhamnosus 4B15) 균주의 스트레스성 질환 예방 또는 개선용 조성물 |
CN116694537A (zh) * | 2023-07-28 | 2023-09-05 | 善恩康生物科技(苏州)有限公司 | 鼠李糖乳酪杆菌及其在制备治疗2型糖尿病产品中的应用 |
CN118291323A (zh) * | 2024-04-02 | 2024-07-05 | 湖南益百益优生物科技有限公司 | 一种改善高血糖症状的鼠李糖乳酪杆菌bn06及其应用 |
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Cited By (6)
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US20230131140A1 (en) * | 2021-10-26 | 2023-04-27 | University Of South Florida | Human origin probiotic lactobacilius rhamnosus hl-200 to reduce leaky gut by metabolizing ethanolamine |
KR20230062143A (ko) | 2021-10-29 | 2023-05-09 | 연세대학교 원주산학협력단 | 락토바실러스 람노서스 q1 유효성분으로 함유하는 탈모 예방 또는 치료용 조성물 |
KR20230061882A (ko) | 2021-10-29 | 2023-05-09 | 서울우유협동조합 | 락토바실러스 람노서스 4B15(Lacticaseibacillus rhamnosus 4B15) 균주의 스트레스성 질환 예방 또는 개선용 조성물 |
CN116694537A (zh) * | 2023-07-28 | 2023-09-05 | 善恩康生物科技(苏州)有限公司 | 鼠李糖乳酪杆菌及其在制备治疗2型糖尿病产品中的应用 |
CN116694537B (zh) * | 2023-07-28 | 2023-10-31 | 善恩康生物科技(苏州)有限公司 | 鼠李糖乳酪杆菌及其在制备治疗2型糖尿病产品中的应用 |
CN118291323A (zh) * | 2024-04-02 | 2024-07-05 | 湖南益百益优生物科技有限公司 | 一种改善高血糖症状的鼠李糖乳酪杆菌bn06及其应用 |
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