KR102292431B1 - 세포내 생물발광 공명 에너지 전이를 이용하여 생물활성제에 의한 세포 표적 결합의 인식 - Google Patents

세포내 생물발광 공명 에너지 전이를 이용하여 생물활성제에 의한 세포 표적 결합의 인식 Download PDF

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KR102292431B1
KR102292431B1 KR1020207024622A KR20207024622A KR102292431B1 KR 102292431 B1 KR102292431 B1 KR 102292431B1 KR 1020207024622 A KR1020207024622 A KR 1020207024622A KR 20207024622 A KR20207024622 A KR 20207024622A KR 102292431 B1 KR102292431 B1 KR 102292431B1
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bioactive agent
fluorophore
leu
gly
bret
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캐롤린 더블유. 히트코
토마스 커클랜드
토마스 마흐라이트
레이첼 에프. 오하나
매트 로버스
키이스 우드
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프로메가 코포레이션
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    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
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    • C12N15/62—DNA sequences coding for fusion proteins
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
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    • G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
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    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]

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KR1020207024622A 2012-12-12 2013-12-12 세포내 생물발광 공명 에너지 전이를 이용하여 생물활성제에 의한 세포 표적 결합의 인식 Active KR102292431B1 (ko)

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US201261736429P 2012-12-12 2012-12-12
US61/736,429 2012-12-12
US201361794461P 2013-03-15 2013-03-15
US61/794,461 2013-03-15
US201361880048P 2013-09-19 2013-09-19
US61/880,048 2013-09-19
PCT/US2013/074765 WO2014093677A1 (en) 2012-12-12 2013-12-12 Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer
KR1020157018230A KR102150479B1 (ko) 2012-12-12 2013-12-12 세포내 생물발광 공명 에너지 전이를 이용하여 생물활성제에 의한 세포 표적 결합의 인식

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KR102292431B1 true KR102292431B1 (ko) 2021-08-20

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KR1020157018230A Active KR102150479B1 (ko) 2012-12-12 2013-12-12 세포내 생물발광 공명 에너지 전이를 이용하여 생물활성제에 의한 세포 표적 결합의 인식

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US (1) US10024862B2 (https=)
EP (2) EP2932267B1 (https=)
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KR (2) KR102292431B1 (https=)
CN (2) CN104871001B (https=)
AU (1) AU2013359166B2 (https=)
BR (1) BR112015013710B1 (https=)
CA (2) CA3174484A1 (https=)
DK (1) DK2932267T3 (https=)
ES (1) ES2803503T3 (https=)
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KR20250004513A (ko) 2023-06-29 2025-01-08 부산대학교 산학협력단 Bret-기반 rab11a 활성 바이오센서 및 이를 포함하는 세포주

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Publication number Priority date Publication date Assignee Title
KR20250004513A (ko) 2023-06-29 2025-01-08 부산대학교 산학협력단 Bret-기반 rab11a 활성 바이오센서 및 이를 포함하는 세포주

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JP7676487B2 (ja) 2025-05-14
CN104871001A (zh) 2015-08-26
EP2932267B1 (en) 2020-05-06
JP2016500263A (ja) 2016-01-12
JP7092691B2 (ja) 2022-06-28
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