KR102205717B1 - 신규 l-트립토판 배출 단백질 변이체 및 이를 이용한 l-트립토판을 생산하는 방법 - Google Patents

신규 l-트립토판 배출 단백질 변이체 및 이를 이용한 l-트립토판을 생산하는 방법 Download PDF

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KR102205717B1
KR102205717B1 KR1020190040397A KR20190040397A KR102205717B1 KR 102205717 B1 KR102205717 B1 KR 102205717B1 KR 1020190040397 A KR1020190040397 A KR 1020190040397A KR 20190040397 A KR20190040397 A KR 20190040397A KR 102205717 B1 KR102205717 B1 KR 102205717B1
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wex
amino acid
tryptophan
seq
leu
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KR20200118334A (ko
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정무영
김현아
서창일
이임상
김지원
김태연
손성광
정기용
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씨제이제일제당 주식회사
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Priority to US17/268,691 priority patent/US11524981B2/en
Priority to EP20784578.5A priority patent/EP3789398A4/en
Priority to CA3105666A priority patent/CA3105666C/en
Priority to MX2021000685A priority patent/MX2021000685A/es
Priority to RU2020139396A priority patent/RU2763603C1/ru
Priority to PCT/KR2020/003855 priority patent/WO2020204427A1/ko
Priority to JP2021517540A priority patent/JP7539871B2/ja
Priority to BR112021000765-2A priority patent/BR112021000765B1/pt
Priority to CN202080004146.7A priority patent/CN112823165B/zh
Publication of KR20200118334A publication Critical patent/KR20200118334A/ko
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Priority to JP2022155934A priority patent/JP2022185040A/ja
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/22—Tryptophan; Tyrosine; Phenylalanine; 3,4-Dihydroxyphenylalanine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/22—Tryptophan; Tyrosine; Phenylalanine; 3,4-Dihydroxyphenylalanine
    • C12P13/227—Tryptophan
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/15—Corynebacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185—Escherichia
    • C12R2001/19—Escherichia coli

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
KR1020190040397A 2019-04-05 2019-04-05 신규 l-트립토판 배출 단백질 변이체 및 이를 이용한 l-트립토판을 생산하는 방법 Active KR102205717B1 (ko)

Priority Applications (11)

Application Number Priority Date Filing Date Title
KR1020190040397A KR102205717B1 (ko) 2019-04-05 2019-04-05 신규 l-트립토판 배출 단백질 변이체 및 이를 이용한 l-트립토판을 생산하는 방법
BR112021000765-2A BR112021000765B1 (pt) 2019-04-05 2020-03-20 Variante de proteína de exportação de l-triptofano e seu uso, polinucleotídeo que codifica a dita variante, vetor, microrganismo que produz l-triptofano e método para produzir l- triptofano
CA3105666A CA3105666C (en) 2019-04-05 2020-03-20 Novel l-tryptophan-exporting protein variant and method for producing l-tryptophan using the same
MX2021000685A MX2021000685A (es) 2019-04-05 2020-03-20 Novedosa variante de proteina exportadora de l-triptofano y procedimiento para producir l-triptofano utilizando el mismo.
RU2020139396A RU2763603C1 (ru) 2019-04-05 2020-03-20 Новый вариант белка-экспортера l-триптофана и способ получения l-триптофана с его применением
PCT/KR2020/003855 WO2020204427A1 (ko) 2019-04-05 2020-03-20 신규 l-트립토판 배출 단백질 변이체 및 이를 이용한 l-트립토판을 생산하는 방법
US17/268,691 US11524981B2 (en) 2019-04-05 2020-03-20 L-tryptophan-exporting protein variant and method for producing L-tryptophan using the same
EP20784578.5A EP3789398A4 (en) 2019-04-05 2020-03-20 Novel L-TRYPTOPHANE EXPORT PROTEIN VARIANT AND METHOD FOR MANUFACTURING L-TRYPTOPHANE USING THEREOF
CN202080004146.7A CN112823165B (zh) 2019-04-05 2020-03-20 新型l-色氨酸输出蛋白变体及使用其生产l-色氨酸的方法
JP2021517540A JP7539871B2 (ja) 2019-04-05 2020-03-20 新規ｌ－トリプトファン排出タンパク質変異体及びそれを用いてｌ－トリプトファンを生産する方法
JP2022155934A JP2022185040A (ja) 2019-04-05 2022-09-29 新規ｌ－トリプトファン排出タンパク質変異体及びそれを用いてｌ－トリプトファンを生産する方法

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Application Number Priority Date Filing Date Title
KR1020190040397A KR102205717B1 (ko) 2019-04-05 2019-04-05 신규 l-트립토판 배출 단백질 변이체 및 이를 이용한 l-트립토판을 생산하는 방법

Publications (2)

Publication Number Publication Date
KR20200118334A KR20200118334A (ko) 2020-10-15
KR102205717B1 true KR102205717B1 (ko) 2021-01-22

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KR1020190040397A Active KR102205717B1 (ko) 2019-04-05 2019-04-05 신규 l-트립토판 배출 단백질 변이체 및 이를 이용한 l-트립토판을 생산하는 방법

Country Status (10)

Country Link
US (1) US11524981B2 (https=)
EP (1) EP3789398A4 (https=)
JP (2) JP7539871B2 (https=)
KR (1) KR102205717B1 (https=)
CN (1) CN112823165B (https=)
BR (1) BR112021000765B1 (https=)
CA (1) CA3105666C (https=)
MX (1) MX2021000685A (https=)
RU (1) RU2763603C1 (https=)
WO (1) WO2020204427A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
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WO2024144283A1 (ko) * 2022-12-28 2024-07-04 씨제이제일제당 (주) 외래 글루타민 신테타제가 도입된 미생물 및 이를 이용한 l-트립토판의 생산 방법

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KR102647745B1 (ko) * 2020-05-27 2024-03-14 씨제이제일제당 주식회사 신규 l-타이로신 배출 단백질 변이체 및 이를 이용한 l-타이로신을 생산하는 방법
KR102617168B1 (ko) * 2020-12-09 2023-12-21 씨제이제일제당 (주) 쉬와넬라 오네이덴시스 유래 단백질을 발현하는 미생물, 및 이를 이용한 l-아미노산 생산 방법
KR102284728B1 (ko) * 2021-01-25 2021-08-02 씨제이제일제당 주식회사 신규한 H(+)/Cl(-) 익스체인지 트랜스포터 변이체 및 이를 이용한 L-트립토판 생산 방법
KR102284725B1 (ko) * 2021-01-25 2021-08-02 씨제이제일제당 주식회사 신규한 페로체라테이즈 변이체 및 이를 이용한 l-트립토판 생산 방법
KR102284726B1 (ko) * 2021-01-25 2021-08-02 씨제이제일제당 주식회사 신규한 타우토머레이즈 pptA 변이체 및 이를 이용한 L-트립토판 생산 방법
KR102284727B1 (ko) * 2021-01-25 2021-08-02 씨제이제일제당 주식회사 신규한 단백질 HtrL 변이체 및 이를 이용한 L-트립토판 생산 방법
KR102284729B1 (ko) * 2021-01-29 2021-08-02 씨제이제일제당 주식회사 신규한 시아네이트 트랜스포터 패밀리 단백질변이체 및 이를 이용한 l-트립토판 생산 방법
KR102688095B1 (ko) * 2021-04-28 2024-07-24 씨제이제일제당 주식회사 변이형 SpoT 단백질 및 이를 이용한 L-아미노산을 생산하는 방법
KR102771798B1 (ko) * 2022-10-24 2025-02-25 대상 주식회사 Qin 프로파지; 단백질 YnfQ 신규 변이체 및 이를 이용한 L-방향족 아미노산의 생산 방법
EP4610272A1 (en) * 2022-10-24 2025-09-03 Daesang Corporation Novel variant of dna-binding transcriptional regulator malt and method for producing l-aromatic amino acid using same
WO2024090884A1 (ko) * 2022-10-24 2024-05-02 대상 주식회사 페닐알라닌:h+ 동시수송체 phep 신규 변이체 및 이를 이용한 l-방향족 아미노산의 생산 방법

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JP2022185040A (ja) 2022-12-13
JP2021525108A (ja) 2021-09-24
US11524981B2 (en) 2022-12-13
RU2763603C1 (ru) 2021-12-30
EP3789398A4 (en) 2021-08-25
BR112021000765A2 (pt) 2021-04-13
CN112823165B (zh) 2024-03-26
US20220009973A1 (en) 2022-01-13
CA3105666A1 (en) 2020-10-08
EP3789398A1 (en) 2021-03-10
BR112021000765B1 (pt) 2022-05-10
CN112823165A (zh) 2021-05-18
CA3105666C (en) 2023-06-27
JP7539871B2 (ja) 2024-08-26
MX2021000685A (es) 2021-03-25
WO2020204427A1 (ko) 2020-10-08
KR20200118334A (ko) 2020-10-15

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