KR102205348B1 - 외인성 항원을 포함하는 인간 시토메갈로바이러스 - Google Patents
외인성 항원을 포함하는 인간 시토메갈로바이러스 Download PDFInfo
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- KR102205348B1 KR102205348B1 KR1020197018702A KR20197018702A KR102205348B1 KR 102205348 B1 KR102205348 B1 KR 102205348B1 KR 1020197018702 A KR1020197018702 A KR 1020197018702A KR 20197018702 A KR20197018702 A KR 20197018702A KR 102205348 B1 KR102205348 B1 KR 102205348B1
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Abstract
이종유래 항원을 포함하는 인간 시토메갈로바이러스 벡터가 개시된다. 상기 벡터는 TR 스트레인으로부터 유래하며, 간시클로비르-민감성이고, 활성인 US2, US3, US6, US7 및 UL131A 유전자를 포함하며, UL82 유전자에 유해 또는 불활성화 돌연변이를 가짐으로써 pp71의 발현을 방지한다.
Description
[관련 출원의 상호 참조]
본 출원은 발명의 명칭이 HUMAN CYTOMEGALOVIRUS COMPRISING EXOGENOUS ANTIGENS인 2014년 7월 16일자 U.S. 가출원 제62/025,348호에 대하여 우선권을 주장하고, 그 개시내용은 전문이 참조로 포함된다.
[기술분야]
일반적으로, 분야는 백신 플랫폼과 연관되어 있다. 더 구체적으로, 분야는 외인성 항원을 발현하는 재조합 인간 시토메갈로바이러스(cytomegalovirus) 벡터와 연관되어 있다.
시토메갈로바이러스 (CMV)-벡터화 백신이 하기의 점에서 독특하다는 것이 동물 실험에서 입증되어 있다: a) 고빈도의 림프외 T 세포 반응 (소위 이펙터 기억 T 세포)을 유도 및 유지하며; b) CMV-양성 숙주를 과-감염시키고; c) 숙주-대-숙주 확산이 결핍되게 된 경우에도 면역원성을 유지함. 또한, 동물 모델 실험에서, 동물 CMV로부터 유래하는 백신 벡터가 감염성 질환 및 암에 대한 보호 면역 반응을 유도한다는 것이 밝혀져 있다 (US 20080199493호; US 20100142823호; US 20130136768호; 및 US 20140141038호; 모두 본원에 참조로 포함됨). 특히 놀라운 것은 붉은털원숭이(rhesus) CMV (RhCMV)-벡터화된 유인원 면역결핍 바이러스 (SIV)-백신이 비-인간 영장류에서 AIDS를 예방할 수 있었을 뿐만 아니라 궁극적으로 해당 동물들을 SIV로부터 치유한다는 발견이다 (문헌 [Hansen SG et al., Nature 502, 100-104 (2013)]; 본원에 참조로 포함됨).
시토메갈로바이러스 백신의 개발에서는 약독화된 스트레인을 사용하는 것이 중요한데, 비약독화 스트레인이 숙주에서 숙주로 확산될 수 있으며, 잠재적으로 적어도 면역손상 개체에서는 병원성일 수 있기 때문이다. 지금까지, 약독화된 인간 CMV (HCMV) 스트레인은 하기에 실패하였었다: a) 잠복 감염을 확립하는 것 (문헌 [Plotkin SA and Huang ES, J Infect Dis 152, 395-397 (1985)]; 본원에 참조로 포함됨); b) 장기-지속 면역을 유도하는 것 (문헌 [Jacobson MA et al., J Clin Virol 35, 332-337 (2006)]; 본원에 참조로 포함됨); c) 이미 자연적으로 CMV에 의해 감염되어 있는 상당 비율의 집단을 재감염시키는 것 (문헌 [Heineman TC et al., J Infect Dis 193, 1350-1360 (2006)]; 본원에 참조로 포함됨); 또는 d) 지속적인 감염을 산출하는 것 (WO2013/036465호; 본원에 참조로 포함됨). 또한, HCMV 게놈의 임상용 스트레인은 시험관 내에서 매우 불안정해서, 섬유모세포에서 성장되는 경우, UL131A의 결실과 같은 섬유모세포 적응(fibroblast adaptation)을 초래한다.
생체 내에서 벡터 기능을 수행하는 능력에 있어서의 조직 배양에 대한 그와 같은 적응의 영향은 거의 알려져 있지 않다. 시험관 내 및 생체 내에서 안정하도록 하는 약독화에 대한 필요성 이외에도, 이와 같은 벡터는 재현성 있는 결과로 제조될 수 있도록 하는 것이 중요하다. 가장 안정한 약독화 전략은 유전자 결실이다. 그러나, 이는 일반적으로 시토메갈로바이러스를 성장시키는 데에 사용되는 초대 세포에서는 달성하기가 어려운 보완 세포주(complementing cell line)의 생성을 필요로 한다.
[발명의 개요]
본원에서 개시되는 것은 HCMV TR 스트레인의 유전적으로 변형된 버전인 HCMV-TR3 유래의 심하게 약독화된 확산-결핍성 (즉 세포 대 세포 확산의 결핍) 벡터이다. 개시되는 벡터는 지속적인 감염을 확립하여 유지하며, 이종유래 항원에 대한 이펙터 기억 T 세포를 유도하여 유지하고, CMV-혈청양성 숙주를 재-감염시킨다. 상기 벡터는 비-CMV 병원체 특이적 항원 또는 종양 항원과 같은 이종유래 항원을 포함한다.
구체적으로, TR3는 간시클로비르-민감성이 되도록 조작되었다. 일 예에서, 이는 활성 UL97 유전자 (TR3의 원래 임상 단리물에서 돌연변이되었던 것)의 첨가에 기인한다. TR3는 TR3의 원래 임상 단리물의 BAC 클로닝 동안 제거되었던 활성 US2, US3, US6 및 US7 유전자를 포함하도록 추가적으로 조작되었다. TR3의 추가적인 버전은 pp71-코딩 UL82 유전자에 유해 (즉 불활성화) 돌연변이를 포함하는데 - 본원에서 이는 TR3Δpp71 또는 달리 TR3ΔUL82로 지칭될 수 있다.
벡터의 추가적인 예에서, 이종유래 항원을 코딩하는 유전자의 발현은 UL82 프로모터 또는 또 다른 바이러스 프로모터 예컨대 UL7, UL38, UL45 또는 US13 프로모터에 의해 추진될 수 있다. 또 다른 예에서는, 바이러스 유전자 프로모터가 이종유래 항원 유전자의 발현을 추진하도록, 이종유래 항원을 코딩하는 다수의 유전자가 UL82 및 또 다른 바이러스 유전자 예컨대 UL7, UL38, UL45 또는 US13 대신 삽입될 수 있다.
또한 본원에서 개시되는 것은 기능성인 pp71 단백질 (UL82 유전자에 의해 코딩됨)이 결핍되어 있는 HCMV의 제조 방법이다. 상기 방법은 기능성 pp71 단백질이 결핍되어 있는 HCMV를 사용하여 세포를 감염시키는 것을 포함하며, 여기서 상기 세포는 DAXX 유전자를 침묵화하는 siRNA를 함유한다. 다른 실시양태에서, 상기 방법은 기능성 pp71 단백질이 결핍되어 있는 HCMV를 사용하여 세포를 감염시키는 것을 포함하며, 여기서 상기 세포에서의 DAXX 유전자의 발현은 관련 기술분야에 알려져 있는 다른 기술에 의해, 예를 들면 RNA 간섭 (예컨대 마이크로RNA 표적화 및 짧은 헤어핀 RNA (shRNA) 표적화), 리보자임 절단, 조건부 또는 유도가능 프로모터에 의한 발현 조절, DAXX 결합 단백질의 발현, 또는 유비퀴틴화 및 분해에 대한 DAXX 또는 DAXX 단백질 복합체의 표적화)에 의해 단백질 또는 RNA 수준에서 하향조절되어 있다. 이러한 방법들을 사용함으로써, 보완(complementation) 없이 효율적으로 HCMV가 제조된다. 상기 세포는 인간 섬유모세포를 포함한 임의의 세포일 수 있다.
본원의 도면 중 일부는 컬러로 제시될 때 더 잘 이해되는데, 특허 출원 공개에서는 그것이 가능하지 않다. 그러나, 본 출원인은 컬러 도면이 원래 개시내용의 일부이며, 차후 진행시 본원 도면의 컬러 버전을 제시할 권리가 확보되어 있다고 생각한다.
도 1a 및 1b는 합쳐서 HCMV TR이 다른 HCMV 스트레인에 비해 잠복성을 확립하고 잠복으로부터 재활성화 (+G-CSF)되는 데에 있어서 탁월하다는 것을 보여준다. 도 1a는 도 1b에 사용되는 HCMV 클론의 게놈 구성 맵이다. HCMV 게놈에는 말단 반복체 (표시된 바와 같은 TRL 및 TRS), 및 고유 장 (UL) 및 고유 단 (US) 영역을 분리하는 내부 반복체 (IRS)가 측접한다. 각 구축물에서의 BAC 카세트의 위치는 B로 표시된 영역으로 표시하였다. HCMV TR의 US 영역에는 BAC-카세트의 삽입으로 인하여 US2-7이 결핍되어 있다. TRΔ4는 US2-7이 결핍되어 있는 것 이외에, 유전자 UL128-UL150도 결핍되어 있다. UL131A 유전자에는 AD169가 결핍되어 있으나, AD169 BAD UL131A에서는 복구된다 (하기 문헌 [Wang and Schenk, 2005]). 톨레도는 UL128의 결실과 함께 UL133-128 영역의 역전(inversion)을 보유한다 (하기 문헌 [Murphy et al., 2003]). 도 1b는 인간 CD34+ 줄기 세포가 이식되어 있으며 표시된 HCMV 스트레인으로 감염된 인간 섬유모세포를 사용하여 복막내로 접종된 NOD/SCID/IL2Rγ-무효(null) (NSG) 마우스의 결과를 요약하는 플롯이다. 감염 4주 후, 과립구 집락-자극 인자 (G-CSF) 처리에 의해 인간 조혈 줄기 세포를 가동화하고, 정량 PCR에 의해 간에서 바이러스 부하를 측정하였다.
도 2는 원래의 HCMV TR 스트레인에 존재하는 오픈 리딩 프레임 (ORF)의 변경을 보여주는 HCMV-TR3 게놈의 그래픽 표시이다. 간시클로비르 민감성을 부여하기 위하여, HCMV TR의 UL97을 HCMV AD169의 것으로 대체하였다. BAC 카세트에는 loxP 부위들이 측접하는데, cre-매개 자기-절단 후에는, 단일 loxP 부위가 게놈에 잔존한다. HCMV-TR BAC에 US2-7이 결핍되어 있기 때문에, HCMV AD169 유래의 상응하는 유전자를 삽입하였다. 말단 (ab 및 c'a) 반복체 및 내부 반복체 (b'a'c)를 나타내었다.
도 3은 HCMV-TR3이 간시클로비르 (GCV)에 민감성이나, HCMV-TR은 그렇지 않다는 것을 보여주는 플롯이다. 성장-중지 인간 태아 섬유모세포 MRC-5 세포를 HCMV TR3, HCMV TB40E 및 원래의 HCMV TR로 감염시키거나 (1 PFU/세포의 MOI), 모의 감염시켰다. 표시될 경우, 감염 90분 후에 비처리 대조군에서 광범위한 바이러스 세포변성 효과가 관찰될 때까지 (감염 4일 후) 점증 농도의 GCV를 사용하여 세포를 처리하였다. 다음에, 세포 배양 상청액을 MRC-5 세포에서의 표준 플라크 감소 검정에 의해 감염성에 대하여 검정하였다. 플라크 수를 약물 농도의 함수로서 플로팅하고, IC50을 측정하였다. 값들은 2회 개별 측정의 평균이다.
도 4a 및 4b는 HCMV-TR3이 놀랍게도 내피 세포를 감염시키는 능력을 유지하며, 다중 계대 후에도 게놈 안정성을 유지한다는 것을 보여준다. 도 4a는 하기를 보여주는 겔 이미지이다: HCMV-TR3 BAC를 MRC-5 세포에서 재구성한 다음, 시험관 내 또는 초대 인간 섬유모세포에서 20회 계대하였다. 계대 1, 5, 10, 15 및 20에서, 감염된 세포로부터 바이러스 DNA를 추출하고, 제한 분해 분석, 및 다중 계대의 결과로서 빈번하게 돌연변이되는 영역인 UL128-131 영역의 PCR 서열분석에 적용하였다 (하기 문헌 [Dargan et al., 2010]). 도 4b는 MRC-5 세포에서의 다중 계대 후 인간 제대 정맥 내피 세포 (HUVEC)에서의 TR3의 감염성을 보여주는 플롯이다. 계대 10에서 정제된 바이러스 모액을 제조하고, MOI = 0.5에서 HUVEC을 감염시키는 데에 사용하였다. 동시에, 대조군으로서 HCMV 실험실 적응 스트레인 AD169를 사용하여서도 HUVEC을 감염시켰다. 감염 5, 10, 15 및 20일 후에 (pi) 상청액 및 세포를 수확하여, MRC-5 세포에서의 플라크 검정에 의해 적정하였다. 시간이 지남에 따른 역가의 증가는 HCMV TR3가 무손상 UL131A-128 영역과 일치하여 HUVEC에서 성장할 수 있는 반면, HCMV AD169는 성장하지 않는다는 것을 표시한다.
도 5는 UL128-131의 존재가 세포-무함유 HCMV-TR3의 수율을 감소시키지 않는다는 것을 보여주는 플롯이다. HCMV-TR3에 의해 0.01의 MOI로 감염된 MRC-5 세포, 및 TR3와 동일하나 UL128-131이 결실된 스트레인 (HCMVΔUL128-131)을 사용하여 다-단계 성장 곡선 분석을 수행하였다. 감염된 세포 및 상청액의 역가는 MRC-5 세포에서 표준 플라크 검정에 의해 감염 2, 5, 10, 15 및 20일 후에 측정하였다.
도 6a는 EF1α 프로모터의 조절하에 있는 SIVgag를 문헌 [Hansen SG et al., Nat Med 15, 293-299 (2009)] (본원에 참조로 포함됨)에 기술되어 있는 바와 같은 BAC 돌연변이유발을 사용하여 HCMV-TR3 게놈으로 삽입하였을 때의 결과를 보여주는 2개 플롯 세트이다. CMV에 대하여 혈청-양성인 붉은털원숭이(Rhesus macaques) (RM)에 105 플라크-형성 단위 (PFU)의 SIVgag를 발현하는 HCMV-TR을 접종하였다. 나타낸 것은 HCMV 용해물 (다이아몬드) 또는 중복 SIVgag (정사각형) 펩티드에 반응하는 말초 혈액 단핵 세포 (PMBC)에서의 % 기억 T 세포이다. 정준(canonical) CM9 펩티드 (원)에 대한 T 세포의 부재에 유의해야 하는데, HCMV에 의해 유도된 T 세포 반응이 RhCMV에 대하여 기술되었던 바와 같은 다른 벡터들의 것과 다르다는 것을 표시한다 (하기 문헌 [Hansen et al., Science 2013]). 좌측의 플롯은 CD4+ T 세포 반응을 나타낸다. 우측의 플롯은 CD8+ T 세포 반응을 나타낸다.
도 6b는 UL128-131이 결실되어 있는 UL78 프로모터 조절하에 HIVgag를 발현하는 HCMV (ΔUL128-131HCMVgag), 또는 UL128-131이 무손상인 UL82 프로모터 조절하에 HIVgag를 발현하는 HCMV (Δpp71HCMVgag)가 접종된 RM에서의 HIVgag-특이적 T 세포 반응을 보여주는 2개 플롯 세트이다. 106 PFU의 ΔUL128-131 벡터를 RM에 접종하였을 경우, HIVgag에 대한 CD4+ 또는 CD8+ T 세포 반응은 관찰되지 않았다. 반면, Δpp71 HCMVgag 벡터에서는 HIVgag-특이적 T 세포 반응이 관찰되었다. 좌측의 플롯은 CD4+ T 세포 반응을 나타내고, 우측의 플롯은 CD8+ T 세포 반응을 나타낸다.
도 7a는 어떻게 야생형 HCMV에 의한 감염 동안 외피 단백질 pp71이 세포 보조억제인자 DAXX를 분해하는지를 예시하는 도면이다. pp71의 부재하에서는, DAXX가 바이러스 유전자 발현 및 그에 따른 용해성 복제를 억제한다. 그러나, DAXX-특이적 siRNA를 사용한 유전자 녹다운에 의해 DAXX mRNA가 제거될 경우, pp71의 부재하에서도 바이러스 유전자 발현이 정상적으로 진행될 수 있다.
도 7b는 DAXX-특이적 siRNA에 의해 형질감염되고, 형질감염 24시간(h) 후에 MOI=0.05로 TR3 및 TR3Δpp71HIVgag에 의해 감염된 MRC-5 세포의 플롯이다. 표시된 감염 후 시간에, 세포 및 상청액을 별도로 수확하고, pp71을 발현하는 보완 세포에서 적정하였다.
도 8a 및 8b는 HCMVTR3ΔUL82(Δpp71)가 인간화된 마우스에서 잠복성을 확립하나 재활성화되어 확산되는 그의 능력은 결핍되어 있다는 것을 보여주는 플롯이다. 양 플롯에 있어서, CD34+ 줄기 세포가 이식된 NOD/SCID/IL2Rγ-무효 (NSG) 마우스에 TR3 또는 TR3ΔUL82 바이러스로 감염된 섬유모세포를 복막내로 접종하였다. 감염 4주 후, G-CSF 처리에 의해 인간 조혈 줄기 세포를 가동화하고, 정량 PCR에 의해 골수 (TR3, 도 8a) 및 간 (TR3ΔUL82, 도 8b)에서 바이러스 부하를 측정하였다.
도 9는 HIVgag를 발현하는 pp71-결실 HCMV-TR3가 비-인간 영장류에서 HIVgag-특이적 이펙터 기억 T 세포를 유도하는 능력을 유지한다는 것을 보여주는 플롯 세트이다. HIVgag를 발현하나 pp71이 결핍되어 있는 HCMV는 UL82(pp71) 유전자를 HIVgag로 대체함으로써 구성하였다. 생성 바이러스는 DAXX siRNA를 사용하여 회수하였다. 106 또는 105 PFU의 생성 바이러스를 RM에 피하로 접종하고, 표시된 일자에 세포내 시토카인 염색에 의해 HIVgag에 대한 T 세포 반응을 측정하였다. 나타낸 것은 중복 HIVgag 펩티드에 반응하는 말초 혈액 단핵 세포 (PMBC)에서의 CD4+ (좌측) 및 CD8+ (중앙) 기억 T 세포의 백분율이다. 우측 패널은 반응하는 T 세포가 이펙터 기억 표현형을 나타낸다는 것을 보여준다.
도 10a는 SIVenv 및 SIVpol 양자를 발현하는 이중 RhCMV 벡터의 결과를 보여주는 6개 플롯 세트이다. 이중 발현 벡터는 먼저 Rh110 (pp71의 RhCMV 상동체)을 SIVenv로 대체함으로써 구성하였다. 다음에, HCMV 유전자 UL7 (Rh19), UL78 (Rh107) 또는 US13 (Rh191)의 상동체들을 SIVpol로 대체하였다. 생성 벡터는 pp71-발현 붉은털원숭이 섬유모세포에서 회수하였다. 5×106 PFU의 각 벡터를 각각 2 마리의 RM에 접종하였다 (1 마리의 RM은 짙은 선으로 나타내고, 다른 RM은 점선으로 나타내었음). CD4+ 및 CD8+ T 세포 반응은 표시된 일자에 SIVpol 또는 SIVenv 중 어느 하나에 상응하는 중복 15량체 펩티드를 사용하여 PBMC에서 측정하였다. T 세포 기억 풀(pool) 내의 % SIV-특이적 T 세포를 나타내었다.
도 10b는 MRC-5 세포를 모의-감염시키거나, 또는 MOI 0.5의 TR3ΔUL7HIVgag, TR3ΔUL45HIVgag 또는 TR3ΔUL78HIVgag를 사용하여 감염시켰을 때의 결과를 보여주는 SDS-PAGE 겔 이미지이다. 단백질 추출물은 감염 96시간 후 (hpi)에 제조하였다. 20 마이크로그램의 단백질을 10 % SDS-PAGE에서 분리하고, 항-Gag (p24) 항체를 사용하여 면역블럿을 설치하였다.
도 11은 EF1α 프로모터 조절하의 SIVgag를 사용한 결과를 보여주는 2개 플롯 세트이다. 문헌 [Hansen SG et al., Nat Med 15, 293-299 (2009)] (본원에 참조로 포함됨)에 기술되어 있는 바와 같이 BAC 돌연변이유발을 사용하여 HCMV-TR3 게놈에 SIVgag를 삽입하였다. CMV에 대하여 혈청-양성인 붉은털원숭이 (RM)에 105 플라크-형성 단위 (PFU)의 SIVgag를 발현하는 HCMV-TR3를 접종하였다. 나타낸 것은 중복 SIVgag 펩티드에 반응하는 말초 혈액 단핵 세포 (PMBC)에서의 % CD4+ (좌측 패널) 및 % CD8+ (우측 패널) T 세포이다. 접종 378일 후를 넘어서면서 플롯이 2 마리의 붉은털원숭이 (Rh31017, Rh31219)에 대하여 안정한 면역 반응을 나타낸다는 것에 유의하라.
도 12는 TR3ΔUL78HCMV/HIVgagΔUL128-130으로 접종된 2 마리 RM의 T 세포 면역 반응을 플로팅하고 있다. UL131A의 결실을 포함하였던 구축물과 달리, UL128-130로 결실을 제한하는 것은 지속적인 CD4+ 및 CD8+ T 세포 반응을 초래한다.
도 13은 일련 범위의 세포 당 감염성 입자에 걸친 DAXX siRNA의 존재 (원) 또는 부재 (다이아몬드)하에서의 야생형 TR3 (정사각형) 대 ΔUL82(pp71)HIVgag의 성장 동역학을 비교하는 플롯이다. DAXX-특이적 siRNA로 형질감염되고 형질감염 24시간 후에 TR3 및 TR3Δpp71HIVgag에 의해 감염된 MRC-5 세포를 siRNA에 의해 기능적으로 보완한 임상적으로는 적절한 낮은 MOI에서 성장 결합이 나타나거나, 또는 DAXX siRNA의 부재하에서는 복제하는 데에 실패하였다. 낮은 MOI에서의 복제의 결핍은 바이러스가 세포 대 세포 확산이 결핍되어 있다는 것을 표시한다. 표시된 감염 후 시간에, 상청액을 수확하고, pp71 보완 조건 (DAXX siRNA 형질감염된 MRC-5 세포)하에서 적정하였다.
도 14는 HCMVTR3ΔUL82(Δpp71)가 인간화된 마우스에서 잠복성은 확립하였으나, 재활성화 및 확산되는 그의 능력은 결핍된다는 것을 입증하는 3개 그래프 세트이다. CD34+ 줄기 세포를 이식한 NOD/SCID/IL2Rγ-무효 (NSG) 마우스에 TR3, TR3ΔUL82 또는 TR3ΔUL82ΔUL128-130 바이러스로 감염된 섬유모세포를 복막내 접종하였다. 감염 4주 후, G-CSF 처리에 의해 인간 조혈 줄기 세포를 가동화하고, 골수 (상부 좌측 패널), 간 (상부 우측 패널) 및 비장 (저부 패널)에서 바이러스 부하를 측정하였다. 정량 PCR을 바탕으로, 총 DNA 마이크로그램수의 함수로서의 상대적 바이러스 카피 수를 플로팅하였다. 과립구 집락 자극 인자 (G-CSF) 부재하에서의 값은 잠복 바이러스 부하를 나타내며, G-CSF 자극 후의 값은 잠복으로부터 출현하는 바이러스의 재활성화를 나타낸다. pp71이 결실된 구축물은 잠복 감염은 확립하였으나, 바이러스 게놈 DNA의 카피수에 의해 측정하였을 때 G-CSF 자극에 반응하는 데에 실패하였다.
도 15는 TR3/HCMVΔpp71(HIVgag) 구축물이 접종된 3 마리 RM의 면역 반응을 특성화하는 3개 플롯 세트이다. 벡터를 성장시킨 후, siRNA의 존재하에 적정하고, 피하 접종용으로 농축하였다. 나타낸 것은 중복 HIVgag 펩티드에 반응하는 말초 혈액 단핵 세포 (PMBC)에서의 CD4+ (좌측 패널) 및 CD8+ (중앙 패널) 기억 T 세포의 백분율이다. 상이한 투여량의 구축물에 대한 반응을 접종 후 294일까지 그래프화하였다. 우측 패널은 Δpp71(HIVgag)TR3/HCMV의 CD8+ 반응이 T-이펙터 기억 표현형과 일치한다는 것을 입증하고 있다.
도 16은 BAC 클론 서열과 비교한 9 계대를 통한 HCMV/TR3ΔUL82(pp71)HIVgag의 서열 정렬을 그래픽으로 도시한다. 오픈 리딩 프레임 (ORF)을 화살표로 도시하였는데, 자기-절단 BAC는 백색 화살표로 도시하였으며, 바이러스 ORF는 회색 화살표로 도시하였고, UL82 ORF를 대체하는 HIVgag 삽입물은 흑색 화살표로 도시하였다. 내부 및 말단 반복체는 회색 타원으로 도시하였다. LOD 1 %로써, 유의성 있는 다형은 관찰되지 않았다.
도 17a 및 17b는 수회의 감염 주기에 걸친 gag 삽입물 발현 및 균질성을 확인해 준다. 도 17a는 ΔUL82(pp71) 구축물에서의 pp71 단백질 발현의 존재, 및 HIVgag(p24) 발현의 존재를 확인해 주는 복합 웨스턴 블럿을 도시한다. HCMV 발현의 양성 대조군 (pp28) 및 베타-액틴에 대한 로딩 대조군을 포함시켰다. 도 17b는 gag 삽입물의 서열이 이러한 초기 계대 동안 생거 서열분석에 의해 검출되는 다형(polymorphism) 없이 안정하다는 것을 보여준다.
도 18은 어떻게 대안적인 삽입 부위 및 프로모터들이 삽입물 안정성에 영향을 줄 수 있는지의 예를 보여주는 플롯이다. 이와 같은 예에서, SIVgag 삽입물을 추진하는 EF1a 프로모터는 UL36 좌위에 위치되었다. 이와 같은 구축물은 바탕 수준을 초과하는 다형의 출현을 나타낸다. 이와 같은 경우에서, G > T 치환의 출현은 정지 코돈을 생성시킴으로써, 벡터화된 항원을 말단절단시킨다.
[서열 목록]
서열식별번호(SEQ ID NO): 1은 HCMV TR3ΔUL82 BAC의 핵산 서열이다.
서열식별번호: 2는 DAXX를 침묵화하는 siRNA 센스 가닥의 핵산 서열이다.
서열식별번호: 3은 DAXX를 침묵화하는 siRNA 안티센스 가닥의 핵산 서열이다.
인간 DAXX mRNA는 수많은 스플라이스 변이(splice variant)를 포함한다. 스플라이스 변이의 예에는 하기 진뱅크(GenBank) 등재물들이 포함된다: 모두 본원에 참조로 포함 AB015051; CR457085; AF006041; NM_001254717.1; NM_001350; NM_001141969; NM_001141970; HQ436529; HQ436528.
도 1a 및 1b는 합쳐서 HCMV TR이 다른 HCMV 스트레인에 비해 잠복성을 확립하고 잠복으로부터 재활성화 (+G-CSF)되는 데에 있어서 탁월하다는 것을 보여준다. 도 1a는 도 1b에 사용되는 HCMV 클론의 게놈 구성 맵이다. HCMV 게놈에는 말단 반복체 (표시된 바와 같은 TRL 및 TRS), 및 고유 장 (UL) 및 고유 단 (US) 영역을 분리하는 내부 반복체 (IRS)가 측접한다. 각 구축물에서의 BAC 카세트의 위치는 B로 표시된 영역으로 표시하였다. HCMV TR의 US 영역에는 BAC-카세트의 삽입으로 인하여 US2-7이 결핍되어 있다. TRΔ4는 US2-7이 결핍되어 있는 것 이외에, 유전자 UL128-UL150도 결핍되어 있다. UL131A 유전자에는 AD169가 결핍되어 있으나, AD169 BAD UL131A에서는 복구된다 (하기 문헌 [Wang and Schenk, 2005]). 톨레도는 UL128의 결실과 함께 UL133-128 영역의 역전(inversion)을 보유한다 (하기 문헌 [Murphy et al., 2003]). 도 1b는 인간 CD34+ 줄기 세포가 이식되어 있으며 표시된 HCMV 스트레인으로 감염된 인간 섬유모세포를 사용하여 복막내로 접종된 NOD/SCID/IL2Rγ-무효(null) (NSG) 마우스의 결과를 요약하는 플롯이다. 감염 4주 후, 과립구 집락-자극 인자 (G-CSF) 처리에 의해 인간 조혈 줄기 세포를 가동화하고, 정량 PCR에 의해 간에서 바이러스 부하를 측정하였다.
도 2는 원래의 HCMV TR 스트레인에 존재하는 오픈 리딩 프레임 (ORF)의 변경을 보여주는 HCMV-TR3 게놈의 그래픽 표시이다. 간시클로비르 민감성을 부여하기 위하여, HCMV TR의 UL97을 HCMV AD169의 것으로 대체하였다. BAC 카세트에는 loxP 부위들이 측접하는데, cre-매개 자기-절단 후에는, 단일 loxP 부위가 게놈에 잔존한다. HCMV-TR BAC에 US2-7이 결핍되어 있기 때문에, HCMV AD169 유래의 상응하는 유전자를 삽입하였다. 말단 (ab 및 c'a) 반복체 및 내부 반복체 (b'a'c)를 나타내었다.
도 3은 HCMV-TR3이 간시클로비르 (GCV)에 민감성이나, HCMV-TR은 그렇지 않다는 것을 보여주는 플롯이다. 성장-중지 인간 태아 섬유모세포 MRC-5 세포를 HCMV TR3, HCMV TB40E 및 원래의 HCMV TR로 감염시키거나 (1 PFU/세포의 MOI), 모의 감염시켰다. 표시될 경우, 감염 90분 후에 비처리 대조군에서 광범위한 바이러스 세포변성 효과가 관찰될 때까지 (감염 4일 후) 점증 농도의 GCV를 사용하여 세포를 처리하였다. 다음에, 세포 배양 상청액을 MRC-5 세포에서의 표준 플라크 감소 검정에 의해 감염성에 대하여 검정하였다. 플라크 수를 약물 농도의 함수로서 플로팅하고, IC50을 측정하였다. 값들은 2회 개별 측정의 평균이다.
도 4a 및 4b는 HCMV-TR3이 놀랍게도 내피 세포를 감염시키는 능력을 유지하며, 다중 계대 후에도 게놈 안정성을 유지한다는 것을 보여준다. 도 4a는 하기를 보여주는 겔 이미지이다: HCMV-TR3 BAC를 MRC-5 세포에서 재구성한 다음, 시험관 내 또는 초대 인간 섬유모세포에서 20회 계대하였다. 계대 1, 5, 10, 15 및 20에서, 감염된 세포로부터 바이러스 DNA를 추출하고, 제한 분해 분석, 및 다중 계대의 결과로서 빈번하게 돌연변이되는 영역인 UL128-131 영역의 PCR 서열분석에 적용하였다 (하기 문헌 [Dargan et al., 2010]). 도 4b는 MRC-5 세포에서의 다중 계대 후 인간 제대 정맥 내피 세포 (HUVEC)에서의 TR3의 감염성을 보여주는 플롯이다. 계대 10에서 정제된 바이러스 모액을 제조하고, MOI = 0.5에서 HUVEC을 감염시키는 데에 사용하였다. 동시에, 대조군으로서 HCMV 실험실 적응 스트레인 AD169를 사용하여서도 HUVEC을 감염시켰다. 감염 5, 10, 15 및 20일 후에 (pi) 상청액 및 세포를 수확하여, MRC-5 세포에서의 플라크 검정에 의해 적정하였다. 시간이 지남에 따른 역가의 증가는 HCMV TR3가 무손상 UL131A-128 영역과 일치하여 HUVEC에서 성장할 수 있는 반면, HCMV AD169는 성장하지 않는다는 것을 표시한다.
도 5는 UL128-131의 존재가 세포-무함유 HCMV-TR3의 수율을 감소시키지 않는다는 것을 보여주는 플롯이다. HCMV-TR3에 의해 0.01의 MOI로 감염된 MRC-5 세포, 및 TR3와 동일하나 UL128-131이 결실된 스트레인 (HCMVΔUL128-131)을 사용하여 다-단계 성장 곡선 분석을 수행하였다. 감염된 세포 및 상청액의 역가는 MRC-5 세포에서 표준 플라크 검정에 의해 감염 2, 5, 10, 15 및 20일 후에 측정하였다.
도 6a는 EF1α 프로모터의 조절하에 있는 SIVgag를 문헌 [Hansen SG et al., Nat Med 15, 293-299 (2009)] (본원에 참조로 포함됨)에 기술되어 있는 바와 같은 BAC 돌연변이유발을 사용하여 HCMV-TR3 게놈으로 삽입하였을 때의 결과를 보여주는 2개 플롯 세트이다. CMV에 대하여 혈청-양성인 붉은털원숭이(Rhesus macaques) (RM)에 105 플라크-형성 단위 (PFU)의 SIVgag를 발현하는 HCMV-TR을 접종하였다. 나타낸 것은 HCMV 용해물 (다이아몬드) 또는 중복 SIVgag (정사각형) 펩티드에 반응하는 말초 혈액 단핵 세포 (PMBC)에서의 % 기억 T 세포이다. 정준(canonical) CM9 펩티드 (원)에 대한 T 세포의 부재에 유의해야 하는데, HCMV에 의해 유도된 T 세포 반응이 RhCMV에 대하여 기술되었던 바와 같은 다른 벡터들의 것과 다르다는 것을 표시한다 (하기 문헌 [Hansen et al., Science 2013]). 좌측의 플롯은 CD4+ T 세포 반응을 나타낸다. 우측의 플롯은 CD8+ T 세포 반응을 나타낸다.
도 6b는 UL128-131이 결실되어 있는 UL78 프로모터 조절하에 HIVgag를 발현하는 HCMV (ΔUL128-131HCMVgag), 또는 UL128-131이 무손상인 UL82 프로모터 조절하에 HIVgag를 발현하는 HCMV (Δpp71HCMVgag)가 접종된 RM에서의 HIVgag-특이적 T 세포 반응을 보여주는 2개 플롯 세트이다. 106 PFU의 ΔUL128-131 벡터를 RM에 접종하였을 경우, HIVgag에 대한 CD4+ 또는 CD8+ T 세포 반응은 관찰되지 않았다. 반면, Δpp71 HCMVgag 벡터에서는 HIVgag-특이적 T 세포 반응이 관찰되었다. 좌측의 플롯은 CD4+ T 세포 반응을 나타내고, 우측의 플롯은 CD8+ T 세포 반응을 나타낸다.
도 7a는 어떻게 야생형 HCMV에 의한 감염 동안 외피 단백질 pp71이 세포 보조억제인자 DAXX를 분해하는지를 예시하는 도면이다. pp71의 부재하에서는, DAXX가 바이러스 유전자 발현 및 그에 따른 용해성 복제를 억제한다. 그러나, DAXX-특이적 siRNA를 사용한 유전자 녹다운에 의해 DAXX mRNA가 제거될 경우, pp71의 부재하에서도 바이러스 유전자 발현이 정상적으로 진행될 수 있다.
도 7b는 DAXX-특이적 siRNA에 의해 형질감염되고, 형질감염 24시간(h) 후에 MOI=0.05로 TR3 및 TR3Δpp71HIVgag에 의해 감염된 MRC-5 세포의 플롯이다. 표시된 감염 후 시간에, 세포 및 상청액을 별도로 수확하고, pp71을 발현하는 보완 세포에서 적정하였다.
도 8a 및 8b는 HCMVTR3ΔUL82(Δpp71)가 인간화된 마우스에서 잠복성을 확립하나 재활성화되어 확산되는 그의 능력은 결핍되어 있다는 것을 보여주는 플롯이다. 양 플롯에 있어서, CD34+ 줄기 세포가 이식된 NOD/SCID/IL2Rγ-무효 (NSG) 마우스에 TR3 또는 TR3ΔUL82 바이러스로 감염된 섬유모세포를 복막내로 접종하였다. 감염 4주 후, G-CSF 처리에 의해 인간 조혈 줄기 세포를 가동화하고, 정량 PCR에 의해 골수 (TR3, 도 8a) 및 간 (TR3ΔUL82, 도 8b)에서 바이러스 부하를 측정하였다.
도 9는 HIVgag를 발현하는 pp71-결실 HCMV-TR3가 비-인간 영장류에서 HIVgag-특이적 이펙터 기억 T 세포를 유도하는 능력을 유지한다는 것을 보여주는 플롯 세트이다. HIVgag를 발현하나 pp71이 결핍되어 있는 HCMV는 UL82(pp71) 유전자를 HIVgag로 대체함으로써 구성하였다. 생성 바이러스는 DAXX siRNA를 사용하여 회수하였다. 106 또는 105 PFU의 생성 바이러스를 RM에 피하로 접종하고, 표시된 일자에 세포내 시토카인 염색에 의해 HIVgag에 대한 T 세포 반응을 측정하였다. 나타낸 것은 중복 HIVgag 펩티드에 반응하는 말초 혈액 단핵 세포 (PMBC)에서의 CD4+ (좌측) 및 CD8+ (중앙) 기억 T 세포의 백분율이다. 우측 패널은 반응하는 T 세포가 이펙터 기억 표현형을 나타낸다는 것을 보여준다.
도 10a는 SIVenv 및 SIVpol 양자를 발현하는 이중 RhCMV 벡터의 결과를 보여주는 6개 플롯 세트이다. 이중 발현 벡터는 먼저 Rh110 (pp71의 RhCMV 상동체)을 SIVenv로 대체함으로써 구성하였다. 다음에, HCMV 유전자 UL7 (Rh19), UL78 (Rh107) 또는 US13 (Rh191)의 상동체들을 SIVpol로 대체하였다. 생성 벡터는 pp71-발현 붉은털원숭이 섬유모세포에서 회수하였다. 5×106 PFU의 각 벡터를 각각 2 마리의 RM에 접종하였다 (1 마리의 RM은 짙은 선으로 나타내고, 다른 RM은 점선으로 나타내었음). CD4+ 및 CD8+ T 세포 반응은 표시된 일자에 SIVpol 또는 SIVenv 중 어느 하나에 상응하는 중복 15량체 펩티드를 사용하여 PBMC에서 측정하였다. T 세포 기억 풀(pool) 내의 % SIV-특이적 T 세포를 나타내었다.
도 10b는 MRC-5 세포를 모의-감염시키거나, 또는 MOI 0.5의 TR3ΔUL7HIVgag, TR3ΔUL45HIVgag 또는 TR3ΔUL78HIVgag를 사용하여 감염시켰을 때의 결과를 보여주는 SDS-PAGE 겔 이미지이다. 단백질 추출물은 감염 96시간 후 (hpi)에 제조하였다. 20 마이크로그램의 단백질을 10 % SDS-PAGE에서 분리하고, 항-Gag (p24) 항체를 사용하여 면역블럿을 설치하였다.
도 11은 EF1α 프로모터 조절하의 SIVgag를 사용한 결과를 보여주는 2개 플롯 세트이다. 문헌 [Hansen SG et al., Nat Med 15, 293-299 (2009)] (본원에 참조로 포함됨)에 기술되어 있는 바와 같이 BAC 돌연변이유발을 사용하여 HCMV-TR3 게놈에 SIVgag를 삽입하였다. CMV에 대하여 혈청-양성인 붉은털원숭이 (RM)에 105 플라크-형성 단위 (PFU)의 SIVgag를 발현하는 HCMV-TR3를 접종하였다. 나타낸 것은 중복 SIVgag 펩티드에 반응하는 말초 혈액 단핵 세포 (PMBC)에서의 % CD4+ (좌측 패널) 및 % CD8+ (우측 패널) T 세포이다. 접종 378일 후를 넘어서면서 플롯이 2 마리의 붉은털원숭이 (Rh31017, Rh31219)에 대하여 안정한 면역 반응을 나타낸다는 것에 유의하라.
도 12는 TR3ΔUL78HCMV/HIVgagΔUL128-130으로 접종된 2 마리 RM의 T 세포 면역 반응을 플로팅하고 있다. UL131A의 결실을 포함하였던 구축물과 달리, UL128-130로 결실을 제한하는 것은 지속적인 CD4+ 및 CD8+ T 세포 반응을 초래한다.
도 13은 일련 범위의 세포 당 감염성 입자에 걸친 DAXX siRNA의 존재 (원) 또는 부재 (다이아몬드)하에서의 야생형 TR3 (정사각형) 대 ΔUL82(pp71)HIVgag의 성장 동역학을 비교하는 플롯이다. DAXX-특이적 siRNA로 형질감염되고 형질감염 24시간 후에 TR3 및 TR3Δpp71HIVgag에 의해 감염된 MRC-5 세포를 siRNA에 의해 기능적으로 보완한 임상적으로는 적절한 낮은 MOI에서 성장 결합이 나타나거나, 또는 DAXX siRNA의 부재하에서는 복제하는 데에 실패하였다. 낮은 MOI에서의 복제의 결핍은 바이러스가 세포 대 세포 확산이 결핍되어 있다는 것을 표시한다. 표시된 감염 후 시간에, 상청액을 수확하고, pp71 보완 조건 (DAXX siRNA 형질감염된 MRC-5 세포)하에서 적정하였다.
도 14는 HCMVTR3ΔUL82(Δpp71)가 인간화된 마우스에서 잠복성은 확립하였으나, 재활성화 및 확산되는 그의 능력은 결핍된다는 것을 입증하는 3개 그래프 세트이다. CD34+ 줄기 세포를 이식한 NOD/SCID/IL2Rγ-무효 (NSG) 마우스에 TR3, TR3ΔUL82 또는 TR3ΔUL82ΔUL128-130 바이러스로 감염된 섬유모세포를 복막내 접종하였다. 감염 4주 후, G-CSF 처리에 의해 인간 조혈 줄기 세포를 가동화하고, 골수 (상부 좌측 패널), 간 (상부 우측 패널) 및 비장 (저부 패널)에서 바이러스 부하를 측정하였다. 정량 PCR을 바탕으로, 총 DNA 마이크로그램수의 함수로서의 상대적 바이러스 카피 수를 플로팅하였다. 과립구 집락 자극 인자 (G-CSF) 부재하에서의 값은 잠복 바이러스 부하를 나타내며, G-CSF 자극 후의 값은 잠복으로부터 출현하는 바이러스의 재활성화를 나타낸다. pp71이 결실된 구축물은 잠복 감염은 확립하였으나, 바이러스 게놈 DNA의 카피수에 의해 측정하였을 때 G-CSF 자극에 반응하는 데에 실패하였다.
도 15는 TR3/HCMVΔpp71(HIVgag) 구축물이 접종된 3 마리 RM의 면역 반응을 특성화하는 3개 플롯 세트이다. 벡터를 성장시킨 후, siRNA의 존재하에 적정하고, 피하 접종용으로 농축하였다. 나타낸 것은 중복 HIVgag 펩티드에 반응하는 말초 혈액 단핵 세포 (PMBC)에서의 CD4+ (좌측 패널) 및 CD8+ (중앙 패널) 기억 T 세포의 백분율이다. 상이한 투여량의 구축물에 대한 반응을 접종 후 294일까지 그래프화하였다. 우측 패널은 Δpp71(HIVgag)TR3/HCMV의 CD8+ 반응이 T-이펙터 기억 표현형과 일치한다는 것을 입증하고 있다.
도 16은 BAC 클론 서열과 비교한 9 계대를 통한 HCMV/TR3ΔUL82(pp71)HIVgag의 서열 정렬을 그래픽으로 도시한다. 오픈 리딩 프레임 (ORF)을 화살표로 도시하였는데, 자기-절단 BAC는 백색 화살표로 도시하였으며, 바이러스 ORF는 회색 화살표로 도시하였고, UL82 ORF를 대체하는 HIVgag 삽입물은 흑색 화살표로 도시하였다. 내부 및 말단 반복체는 회색 타원으로 도시하였다. LOD 1 %로써, 유의성 있는 다형은 관찰되지 않았다.
도 17a 및 17b는 수회의 감염 주기에 걸친 gag 삽입물 발현 및 균질성을 확인해 준다. 도 17a는 ΔUL82(pp71) 구축물에서의 pp71 단백질 발현의 존재, 및 HIVgag(p24) 발현의 존재를 확인해 주는 복합 웨스턴 블럿을 도시한다. HCMV 발현의 양성 대조군 (pp28) 및 베타-액틴에 대한 로딩 대조군을 포함시켰다. 도 17b는 gag 삽입물의 서열이 이러한 초기 계대 동안 생거 서열분석에 의해 검출되는 다형(polymorphism) 없이 안정하다는 것을 보여준다.
도 18은 어떻게 대안적인 삽입 부위 및 프로모터들이 삽입물 안정성에 영향을 줄 수 있는지의 예를 보여주는 플롯이다. 이와 같은 예에서, SIVgag 삽입물을 추진하는 EF1a 프로모터는 UL36 좌위에 위치되었다. 이와 같은 구축물은 바탕 수준을 초과하는 다형의 출현을 나타낸다. 이와 같은 경우에서, G > T 치환의 출현은 정지 코돈을 생성시킴으로써, 벡터화된 항원을 말단절단시킨다.
[서열 목록]
서열식별번호(SEQ ID NO): 1은 HCMV TR3ΔUL82 BAC의 핵산 서열이다.
서열식별번호: 2는 DAXX를 침묵화하는 siRNA 센스 가닥의 핵산 서열이다.
서열식별번호: 3은 DAXX를 침묵화하는 siRNA 안티센스 가닥의 핵산 서열이다.
인간 DAXX mRNA는 수많은 스플라이스 변이(splice variant)를 포함한다. 스플라이스 변이의 예에는 하기 진뱅크(GenBank) 등재물들이 포함된다: 모두 본원에 참조로 포함 AB015051; CR457085; AF006041; NM_001254717.1; NM_001350; NM_001141969; NM_001141970; HQ436529; HQ436528.
용어:
본원에서 사용될 때, "항원"이라는 용어는 대상체에서 면역 반응을 유도할 수 있는 통상적으로 단백질인 물질을 지칭한다. 상기 용어는 또한 일단 대상체에 투여되고 나면 (직접적인 것, 또는 단백질을 코딩하는 뉴클레오티드 서열 또는 벡터를 대상체에게 투여하는 것 중 어느 하나에 의함) 그 단백질에 대하여 유도되는 체액 및/또는 세포 유형의 면역 반응을 야기할 수 있다는 점에서 면역학적으로 활성인 단백질을 지칭한다.
본원에서 사용될 때, "뉴클레오티드 서열" 및 "핵산 서열"이라는 용어는 비제한적으로 전령 RNA (mRNA), DNA/RNA 혼성체 또는 합성 핵산을 포함한 데옥시리보핵산 (DNA) 또는 리보핵산 (RNA) 서열을 지칭한다. 핵산은 단일-가닥, 또는 부분적 또는 완전 이중-가닥 (이중체)일 수 있다. 이중체 핵산은 단일이중체 또는 이종이중체일 수 있다.
본원에서 사용될 때, "소형 간섭 RNA" ("siRNA") (관련 기술분야에서는 "짧은 간섭 RNA"로도 지칭됨)라는 용어는 약 10-50개 뉴클레오티드 길이 ("뉴클레오티드"라는 용어는 뉴클레오티드 유사체를 포함함), 바람직하게는 약 15-25개 뉴클레오티드 사이 길이, 예컨대 약 20-24개 또는 21-23개 뉴클레오티드 길이, 더욱 바람직하게는 약 15, 16, 17, 18, 19, 20, 21, 22, 23, 24 또는 25개 뉴클레오티드 길이의 RNA 작용제, 바람직하게는 이중-가닥 작용제를 지칭하는 것으로써, 임의적으로 상기 가닥은 예를 들면 RNA 간섭을 유도하거나 매개할 수 있는 1, 2 또는 3개의 돌출형(overhanging) 뉴클레오티드들 (또는 뉴클레오티드 유사체들)을 포함하는 돌출형 말단을 가진다. 자연-발생 siRNA는 세포의 RNAi 기구 (예컨대 다이서(Dicer) 또는 그의 상동체)에 의해 더 긴 dsRNA 분자 (예컨대 >25개 뉴클레오티드 길이)로부터 생성된다.
"단백질", "펩티드", "폴리펩티드" 및 "아미노산 서열"이라는 용어는 본원에서 호환가능하게 사용되어 임의의 길이를 가지는 아미노산 잔기들의 중합체를 지칭한다. 상기 중합체는 선형 또는 분지형일 수 있으며, 변형된 아미노산 또는 아미노산 유사체를 포함할 수 있고, 아미노산이 아닌 다른 화학적 잔기가 거기에 개재될 수 있다. 상기 용어는 또한 자연적으로, 또는 개재; 예를 들면 디술피드 결합 형성, 글리코실화, 지질화, 아세틸화, 인산화, 또는 임의의 다른 조작 또는 변형, 예컨대 표지 또는 생체활성 성분과의 접합에 의해 변형되어 있는 아미노산 중합체를 포괄한다.
본원에서 사용될 때, "재조합"이라는 용어는 자연에서는 발생하지 않는 뉴클레오티드 또는 단백질 분자로 이어지는, 재조합 DNA 기술의 사용을 통하여 생성된 뉴클레오티드 또는 단백질 분자를 의미한다. 재조합 핵산의 일 예는 이종유래 (비-CMV) 항원을 발현하는 HCMV 벡터를 코딩하는 핵산이다.
본원에서 사용될 때, "벡터"라는 용어는 CMV 바이러스와 같은 바이러스를 포함하여, 하나의 환경으로부터 또 다른 환경으로의 핵산 분자의 전달을 가능케 하거나 용이하게 하는 임의의 생물학적 분자를 포괄한다.
단백질 및 그것을 코딩하는 핵산은 본원에서 예시 및 기술되는 정확한 서열과 다른 수 있는 것으로 이해되어야 한다. 이에 따라, 본 발명은 다른 HCMV 벡터가 여전히 이종유래 항원에 대한 면역 반응을 생성시킬 수 있는 동시에, a) 고빈도의 림프외 이펙터 기억 T 세포 반응 (소위 이펙터 기억 T 세포)을 유도 및 유지하며; b) CMV-양성 개체를 재감염시키고; c) 면역원성을 유지하면서도 확산-결핍 (즉 하나의 대상체 또는 숙주로부터 또 다른 대상체 또는 숙주로의 확산의 결핍)으로 유지되는 한, 나타낸 서열에 있어서의 결실, 첨가, 말단절단 및 치환도 고려한다.
이와 관련하여, 치환은 특성상 보존성, 즉 아미노산 계열 내에서 이루어지는 치환일 수 있다. 예를 들면, 아미노산들은 일반적으로 하기 4개 계열로 나누어진다: (1) 산성 -- 아스파르테이트 및 글루타메이트; (2) 염기성 -- 리신, 아르기닌, 히스티딘; (3) 비-극성 -- 알라닌, 발린, 류신, 이소류신, 프롤린, 페닐알라닌, 메티오닌, 트립토판; 및 (4) 비하전 극성 -- 글리신, 아스파라긴, 글루타민, 시스테인, 세린, 트레오닌, 티로신. 페닐알라닌, 트립토판 및 티로신은 때로는 방향족 아미노산으로 분류된다. 이소류신 또는 발린을 사용한 류신의 단발적인 대체 또는 그 반대의 경우; 글루타메이트를 사용한 아스파르테이트의 단발적인 대체 또는 그 반대의 경우; 세린을 사용한 트레오닌의 단발적인 대체 또는 그 반대의 경우; 또는 구조적으로 관련되어 있는 아미노산을 사용한 아미노산의 유사한 보존성 대체는 생물학적 활성에 대하여 중대한 효과를 가지지 않게 된다는 것은 합리적으로 예측가능하다. 따라서, 예시 및 기술된 서열과 실질적으로 동일한 아미노산 서열을 가지나 벡터의 활성에는 실질적으로 영향을 주지 않는 미미한 아미노산 치환을 보유하는 단백질은 본 발명의 영역에 속하는 것이다.
대안적으로, 상동체는 기술된 단백질 또는 핵산 서열에 대비한 % 상동성 기준으로 표현될 수 있다. 상동체는 본원에서 기술되는 HCMV 벡터 및/또는 이종유래 항원에 대하여 적어도 50 %, 적어도 55 %, 적어도 60 %, 적어도 65 %, 적어도 70 %, 적어도 75 %, 적어도 80 %, 적어도 85 %, 적어도 86 %, 적어도 87 %, 적어도 88 %, 적어도 89 %, 적어도 90 %, 적어도 91 %, 적어도 92 %, 적어도 93 %, 적어도 94 %, 적어도 95 %, 적어도 96 %, 적어도 97 %, 적어도 98 % 또는 적어도 99 %의 상동성 또는 동일성을 가질 수 있다.
서열 동일성 또는 상동성은 중복 및 동일성은 최대화하면서 서열 간극은 최소화하도록 정렬되었을 때의 서열들을 비교하는 것에 의해 측정될 수 있다. 구체적으로, 서열 동일성은 수많은 수학적 알고리즘들 중 어느 것을 사용하여 측정될 수 있다. 2개 서열의 비교에 사용되는 수학적 알고리즘의 비제한적인 예는 문헌 [Karlin & Altschul, Proc. Natl. Acad. Sci. USA 87, 2264-2268 (1990)]의 알고리즘으로써, 문헌 [Karlin & Altschul, Proc. Natl. Acad. Sci. USA 90, 5873-5877 (1993)]에서 변형되었다.
서열의 비교에 사용되는 수학적 알고리즘의 또 다른 예는 문헌 [Myers & Miller, CABIOS 4, 11-17 (1988)]의 알고리즘이다. 이와 같은 알고리즘은 GCG 서열 정렬 소프트웨어 패키지의 일부인 얼라인(ALIGN) 프로그램 (버전 2.0)에 통합되어 있다. 아미노산 서열을 비교하기 위하여 얼라인 프로그램을 이용하는 경우에는, PAM120 중량 잔기 표, 12의 간극 길이 벌점, 및 4의 간극 벌점이 사용될 수 있다. 국소적 서열 유사성 및 정렬의 영역을 확인하기 위한 또 다른 유용한 알고리즘은 문헌 [Pearson & Lipman, Proc. Natl. Acad. Sci. USA 85, 2444-2448 (1988)]에 기술되어 있는 바와 같은 파스타(FASTA) 알고리즘이다.
블라스트(BLAST) 알고리즘을 사용하는 것들을 포함하여 생물학적 서열을 비교하는 데에 사용되는 방법의 다른 예는 US 국립 생물공학 정보 센터(National Center for Biotechnology Information) 웹사이트 (http://www.ncbi.nlm.nih.gov/)에서 용이하게 입수가능하다.
HCMV 벡터
본원에서 개시되는 것은 인간 시토메갈로바이러스 (HCMV) 벡터이다. 상기 벡터는 대상체에서 대상체로의 바이러스 확산 (즉 세포 대 세포 확산)을 예방하고 또한 이미 자연적으로 HCMV에 의해 감염되어 있는 대상체를 지속적으로 감염시키도록 조작되어 있다. 상기 벡터는 이종유래 항원에 대한 지속적인 면역 반응을 생성시키며, 약물인 간시클로비르에 대하여 민감성이다.
구체적인 예에서, 상기 벡터는 HCMV TR 스트레인으로부터 유래하며, 활성인 UL97 유전자 (원래의 TR 임상 단리물에는 존재하지 않음)는 물론, 활성인 US2, US3, US6 및 US7 유전자 (클로닝 동안 원래의 TR-BAC로부터 제거됨)를 포함하도록 조작되어 있다. 이러한 변화들을 포함하는 TR 스트레인 벡터의 일 예는 본원에서 TR3로 지칭된다. TR3는 AD169 스트레인으로부터의 UL97은 물론, US2, US3, US6 및 US7 유전자를 포함한다. 일부 실시양태에서, HCMV TR 스트레인으로부터 유래하는 상기 벡터는 추가적으로 활성인 UL131A 유전자를 포함한다. TR3는 무손상 UL131A 유전자를 포함한다.
추가적인 TR3 변이는 UL82 (pp71 단백질을 코딩함), UL7, UL45, UL78 및/또는 US13을 포함한 1종 이상의 다른 바이러스 유전자에 유해 또는 불활성화 돌연변이를 가진다. 상기 유해 또는 불활성화 돌연변이는 유전자의 코딩 서열 및/또는 프로모터의 부분적이거나 전체적인 결실을 포함하는 돌연변이를 포함하여, 유전자에 의해 코딩되는 단백질의 기능 결핍을 초래하는 임의의 돌연변이일 수 있다. 유해 또는 불활성화 돌연변이에는 유전자에 의해 코딩되는 단백질의 기능 결핍을 초래하는 유전자의 코딩 서열 및/또는 프로모터의 점 돌연변이 및 프레임시프트 돌연변이도 포함된다.
TR3 변이는 또한 병원체 특이적 항원 또는 종양 항원과 같은 이종유래 항원을 발현할 수 있다. 이러한 이종유래 항원은 UL82, UL7, UL45, UL78 및/또는 US13 프로모터 또는 HCMV 조기-발현 프로모터(immediate-early promoter)를 포함하여, 내인성 HCMV 프로모터를 포함한 임의의 프로모터에 의해 발현될 수 있다. 관련 TR3 변이에서, 이종유래 항원은 바이러스 UL82, UL7, UL45, UL78 및/또는 US13 유전자를 대체한다. 또 다른 관련 TR3 변이에서, 제1 이종유래 항원은 UL82 유전자를 대체하며, 제2 이종유래 항원은 바이러스 UL7, UL45, UL78 또는 US13 유전자를 대체한다.
TR3 변이의 다른 예에서는, HCMV가 아닌 다른 CMV (예컨대 MCMV-IE 또는 RhCMV-IE) 유래의 프로모터, CMV가 아닌 다른 헤르페스바이러스 유래의 프로모터, 헤르페스바이러스가 아닌 다른 바이러스 유래의 프로모터, 또는 EF1α와 같은 비-바이러스 프로모터를 가지는 이종유래 항원이 제공된다.
일부 실시양태에서, 프로모터는 최소 프로모터에 해당하는 DNA 서열 및 상류 조절 서열의 결합을 포함한다. 최소 프로모터에는 CAP 부위 더하기 TATA 박스가 포함된다. 이들은 기본적인 비조절 전사를 위한 최소한의 서열이다. 상류 조절 서열에는 인핸서 서열과 같은 상류 요소들이 포함된다. 말단절단된 프로모터는 전체-길이 프로모터 중 소정 일부가 제거되어 있는 프로모터이다.
역시 본원에서 개시되는 것은 본원에서 기술되는 HCMV 벡터들 중 어느 것을 코딩하는 핵산이다. 대표적인 핵산 서열이 제공되기는 하지만, 관련 기술분야 통상의 기술자라면, 유전자 코드에 있어서의 중축성(degeneracy)으로 인하여 많은 서로 다른 핵산 서열들이 동일한 단백질 서열을 코딩할 수 있다는 것을 알 수 있을 것이다. 역시 개시되는 것은 상기 HCMV 벡터, 및/또는 HCMV 벡터를 코딩하는 핵산 서열을 포함하는 세포이다. 그와 같은 세포는 포유동물 또는 인간 세포, 예컨대 인간 태아 섬유모세포 및 기타 세포일 수 있다. 일부 예에서, 세포는 DAXX 유전자와 같은 특정 유전자의 발현을 침묵화하는 siRNA를 발현하도록 조작될 수 있다.
본원에서 추가적으로 개시되는 것은 세포 (예컨대 단리된 세포)에서의 약독화된 HCMV 벡터의 제조 방법이다. 상기 방법은 약독화된 HCMV 벡터를 사용하여 세포를 감염시키는 것을 포함한다. 상기 세포는 그렇지 않으면 약독화된 HCMV 벡터가 세포에서 성장하는 것을 막게 되는 유전자를 침묵화하는 siRNA에 의해 형질감염되거나, 그것을 발현한다. 일 예에서, HCMV 벡터는 pp71에서의 결실과 같은 유해 또는 불활성화 돌연변이를 포함하며, siRNA는 DAXX 유전자의 발현을 침묵화한다. 역시 개시되는 것은 세포에서의 DAXX 유전자의 발현이 관련 기술분야에 알려져 있는 다른 기술에 의해, 예를 들면 RNA 간섭 (예컨대 마이크로RNA 표적화 및 짧은 헤어핀 RNA (shRNA) 표적화), 리보자임 절단, 조건부 또는 유도가능 프로모터에 의한 발현 조절, DAXX 결합 단백질의 발현, 또는 유비퀴틴화 및 분해에 대한 DAXX 또는 DAXX 단백질 복합체의 표적화)에 의해 단백질 또는 RNA 수준에서 하향조절되는, 세포 (예컨대 단리된 세포)에서 기능성인 pp71 단백질이 결핍되는 약독화 HCMV 벡터의 제조 방법이다.
본원에서 기술되는 유형의 위치-지정 돌연변이는 합성 올리고뉴클레오티드를 사용하여 도입될 수 있다. 이러한 올리고뉴클레오티드는 원하는 돌연변이 부위에 측접하는 뉴클레오티드 서열을 포함한다. 적합한 방법에 대해서는 문헌 [Morinaga et al., Biotechnology 2, 646-649 (1984)]에 개시되어 있다. 효소-코딩 뉴클레오티드 서열에 돌연변이를 도입하는 또 다른 방법은 문헌 [Nelson and Long, Analytical Biochemistry 180, 147-151 (1989)]에 기술되어 있다. BAC에 있어서의 위치 지정 돌연변이유발 방법에 대해서는 문헌 [Chadburn A et al., Histopathology 53, 513-524 (2008)]; [Lee E et al., Genomics 73, 56-65 (2001)]; 및 [Yu D et al., Proc Natl Acad Sci USA 97, 5978-5983 (2000)]에 기술되어 있는 바, 모두 본원에 참조로 포함된다.
RNA 간섭 (RNAi)은 이중-가닥 RNA (dsRNA)의 직접적인 도입에 의해 유도되는 전사 후 유전자 침묵화 방법으로써, 다양한 생물체에서 특정 유자의 발현을 녹 아웃(knock out)하는 데에 유용한 도구로 출현한 것이다. RNAi에 대해서는 문헌 [Fire et al., Nature 391, 806-811 (1998)] (본원에 참조로 포함됨)에 기술되어 있다. 한 가지 그와 같은 방법은 형질감염에 의한 세포에의 siRNA (소형 간섭 RNA)의 도입을 포함한다. 특정 플라스미드 벡터 시스템과 같은 다른 시스템은 세포에서 안정한 siRNA 발현을 초래한다 (예를 들면 pSUPER 시스템 - 문헌 [Brummelkamp TR et al., Science 296, 550-553 (2002)]; 본원에 참조로 포함됨). 임의의 유전자를 효율적으로 침묵화할 수 있는 siRNA의 설계 방법에 대해서는 관련 기술분야에 알려져 있다.
이종유래 항원
이종유래 항원은 HCMV에서는 원래 발현되지 않는 임의의 단백질로부터 유래할 수 있으며, 병원체 특이적 항원, 종양 항원, 마커 (예컨대 형광 단백질 또는 효소), 성장 인자, 융합 단백질, 또는 그에 대하여 면역 반응이 생성될 수 있는 임의의 다른 단백질 또는 그의 단편 (예컨대 MHC 클래스 I 또는 클래스 II 제한 펩티드)이 포함된다.
본원에서 기술되는 HCMV 벡터 중 이종유래 항원은 병원체 특이적 항원일 수 있다. 예를 들면, 바이러스 병원체 유래의 단백질이 사용될 수 있다. 바이러스 병원체에는 아데노바이러스, 콕사키에바이러스, A형 간염 바이러스, 폴리오바이러스, 리노바이러스, 단순 헤르페스 유형 1, 단순 헤르페스 유형 2, 바리셀라-조스터 바이러스, 엡스타인-바르 바이러스, 카포시 육종 헤르페스바이러스, B형 간염 바이러스, C형 간염 바이러스, 황열병 바이러스, 뎅기 바이러스, 웨스트 나일 바이러스, 인간 면역결핍 바이러스 (HIV), 인플루엔자 바이러스, 홍역 바이러스, 볼거리 바이러스, 파라인플루엔자 바이러스, 호흡기 세포융합 바이러스, 인간 폐렴후바이러스, 인간 파필로마바이러스, 광견병 바이러스, 풍진 바이러스, 인간 보카바이러스 및 파르보바이러스 B19가 포함되나, 이에 제한되는 것은 아니다. 일부 실시양태에서, HCMV 벡터 중 이종유래 항원은 gag, pol, env, rev, tat 및 nef를 포함한 HIV 항원일 수 있다. 유리하게는, 상기 HIV 항원에는 모두 본원에 참조로 포함되는 U.S. 공개 제2008/0199493 A1호 및 2013/0136768 A1호에 논의되어 있는 HIV 항원이 포함되나, 이에 제한되는 것은 아니다.
대안적으로, 이종유래 항원은 세균 병원체 유래의 단백질일 수 있다. 세균 병원체에는 하기가 포함된다: 보르데텔라 페르투씨스(Bordetella pertussis), 보렐리아 부르그도르페리(Borrelia burgdorferi), 브루셀라 아보르투스(Brucella abortus), 브루셀라 카니스(Brucella canis), 브루셀라 멜리텐시스(Brucella melitensis), 브루셀라 수이스(Brucella suis), 캄필로박터 제주니(Campylobacter jejuni), 클라미디아 뉴모니아에(Chlamydia pneumoniae), 클라미디아 트라코마티스(Chlamydia trachomatis), 클라미도필라 프시타시(Chlamydophila psittaci), 클로스트리듐 보툴리눔(Clostridium botulinum), 클로스트리듐 디피실레(Clostridium difficile), 클로스트리듐 페르프린젠스(Clostridium perfringens), 클로스트리듐 테타니(Clostridium tetani), 코리네박테리움 디프테리아에(Corynebacterium diphtheriae), 엔테로코쿠스 파에칼리스(Enterococcus faecalis), 엔테로코쿠스 파에시움(Enterococcus faecium), 에셰리키아 콜리(Escherichia coli), 프란시셀라 툴라렌시스(Francisella tularensis), 하에모필루스 인플루엔자에(Haemophilus influenzae), 헬리코박터 파일로리(Helicobacter pylori), 레지오넬라 뉴모필라(Legionella pneumophila), 렙토스피라 인테로간스(Leptospira interrogans), 리스테리아 모노시토제네스(Listeria monocytogenes), 마이코박테리움 레프라에(Mycobacterium leprae), 마이코박테리움 튜베르큘로시스(Mycobacterium tuberculosis), 마이코박테리움 울세란스(Mycobacterium ulcerans), 마이코플라스마 뉴모니아에(Mycoplasma pneumoniae), 네이쎄리아 고노로에아에(Neisseria gonorrhoeae), 네이쎄리아 메닝기티디스(Neisseria meningitidis), 슈도모나스 아에루지노사(Pseudomonas aeruginosa), 리켓챠 리켓치이(Rickettsia rickettsii), 살모넬라 타이피(Salmonella typhi), 살모넬라 타이피무리움(Salmonella typhimurium), 쉬겔라 손네이(Shigella sonnei), 스태필루코쿠스 아우레우스(Staphylococcus aureus), 스태필루코쿠스 에피데르미디스(Staphylococcus epidermidis), 스태필루코쿠스 사프로피티쿠스(Staphylococcus saprophyticus), 스트렙토코쿠스 아갈락티아에(Streptococcus agalactiae), 스트렙토코쿠스 뉴모니아에(Streptococcus pneumoniae), 스트렙토코쿠스 파이오제네스(Streptococcus pyogenes), 트레포네마 팔리둠(Treponema pallidum), 비브리오 콜레라(Vibrio cholera) 및 여시니아 페스티스(Yersinia pestis).
대안적으로, 이종유래 항원은 기생성 생물체 유래의 단백질일 수 있다. 기생성 생물체에는 가시아메바, 바베시아증, 대장섬모충증, 블라스토시스토시스, 콕시디오이데스, 디엔트아메바증, 아메바증, 편모충, 포자충증, 리슈만편모충증, 원발성 아메바 수막뇌염 (PAM), 말라리아, 리노스포리듐증, 톡소포자충증, 기생성 폐렴, 트리코모나스증, 수면병 및 샤가병과 같은 질환을 야기하는 원생동물이 포함되나, 이에 제한되는 것은 아니다.
대안적으로, 이종유래 항원은 원충 생물체 유래의 단백질일 수 있다. 원충 생물체에는 하기가 포함되나, 이에 제한되는 것은 아니다: 구충, 회충, 촌충, 메디나충, 간 흡충, 장 흡충, 폐 흡충, 쉬스토소모사(Schistosomosa) 및 편충.
대안적으로, 이종유래 항원은 종양 유래의 단백질일 수 있다.
이종유래 항원은 코돈 최적화될 수 있다. HIV 및 기타 렌티바이러스를 포함한 많은 바이러스들이 많은 수의 희귀 코돈을 사용하고 있으므로, 이들 코돈을 원하는 대상체 (예를 들면 인간)에서 통상적으로 사용되는 코돈에 상응하도록 변경하는 것에 의해, 항원의 발현 강화가 달성될 수 있다. 예를 들면, HIV 단백질에서 사용되는 희귀 코돈은 고도로 발현되는 인간 유전자에서 빈번하게 출현하는 것으로 돌연변이될 수 있다 (문헌 [Andre et al., J Virol 72, 1497-1503, (1998)]). 또한, 항원은 병원체의 여러 스트레인들 유래의 컨센서스 서열, 또는 그로부터의 서열 단편을 포함하는 모자이크 항원일 수 있다.
면역원성 조성물:
본원에서 개시되는 것은 개시되는 재조합 HCMV 벡터 및 제약상 허용되는 담체 또는 희석제를 함유하는 면역원성 조성물이다. 재조합 HCMV 벡터를 함유하는 면역원성 조성물은 면역학적 반응을 도출한다. 상기 반응은 반드시는 아니지만 보호성이다. 백신 조성물은 일반적으로 면역학적 기억의 발생과 연관되어 있는 보호 반응을 도출한다.
대상체에서 면역학적 반응을 유도하는 방법 역시 개시된다. 그와 같은 방법은 개시되는 재조합 HCMV 벡터 및 제약상 허용되는 담체 또는 희석제를 포함하는 면역원성 또는 백신 조성물을 대상체에게 투여하는 것을 포함한다. 본 명세서의 목적상, "대상체"라는 용어에는 모든 동물 및 인간이 포함된다.
상기 면역원성 또는 백신 조성물은 비경구 경로 (진피내, 근육내 또는 피하)를 통하여 투여될 수 있다. 다른 투여는 점막 경로, 예컨대 경구, 비내, 생식기내 등을 통할 수 있다.
면역원성 또는 백신 조성물은 제약 관련 기술분야의 통상의 기술자에게 잘 알려져 있는 표준 기술에 따라 제제화 및 투여될 수 있다. 조성물은 단독으로 투여될 수 있거나, 또는 다른 HCMV 벡터 또는 다른 면역원성 백신 또는 치료용 조성물과 함께 공동-투여 또는 순차 투여될 수 있다.
그와 같은 조성물의 예에는 주사가능 투여 - 예를 들면 비경구, 피하, 진피내, 근육내 또는 정맥내 투여 -를 위한 제제와 같은 액체 제제, 예컨대 멸균 현탁액 또는 에멀션이 포함된다. 그와 같은 조성물에서, HCMV 벡터는 적합한 담체, 희석제 또는 부형제 예컨대 멸균수, 생리 식염수, 글루코스 등과의 혼합물이다.
면역원성 또는 백신 조성물은 아주반트를 함유할 수 있다. 알룸 (알루미늄 포스페이트 또는 알루미늄 히드록시드)이 전형적인 아주반트이다. 사포닌 및 그의 정제된 성분 퀼(Quil) A, 프로인트 완전 아주반트(Freund's complete adjuvant), 프로인트 불완전 아주반트(Freund's incomplete adjuvant) 및 기타 아주반트들이 연구 및 수의 적용분야에서 종종 사용된다.
조성물은 주사가능 투여 또는 다른 투여용의 단일 투약 형태로 포장될 수 있는데, 유효 투약량 및 투여 경로는 조성물의 특성, 발현 생성물의 특성 및 기타 인자들에 의해 결정된다. 개시되는 HCMV 벡터의 투약량은 102 pfu 초과, 103 pfu 초과, 104 pfu 초과, 105 pfu 초과, 106 pfu 초과 또는 107 pfu 초과의 투약량을 포함한 플라크 형성 단위 (pfu)로 표현될 수 있다.
[실시예]
하기의 실시예들은 개시되는 방법의 예시이다. 본 개시에 비추어, 관련 기술분야의 통상의 기술자라면, 개시되는 방법의 이들 실시예 및 기타 실시예들의 변이가 과도한 실험 없이도 가능하게 된다는 것을 알고 있을 것이다.
실시예 1 - HCMV-TR3 벡터 플랫폼
이펙터 기억 T 세포-유도 CMV 벡터의 임상적 사용은 유전적으로 안정하며 지속적인 감염을 유지하나 HCMV가 병원성이 될 수 있는 면역손상 대상체로 확산되는 능력은 결핍되어 있는 벡터를 요구한다. 임상 시험에 들어간 HCMV 변이를 위한 이전의 약독화 전략은 섬유모세포에서의 바이러스의 연속 계대(serial passaging) (문헌 [Plotkin SA et al., J Infect Dis 134, 470-475 (1976)]; 본원에 참조로 포함됨), 약독화된 것의 비-약독화 HCMV 스트레인과의 재조합 (상기 문헌 [Heineman J et al. 2006]), 또는 복제-결핍 재조합 벡터의 생성 (WO2013/036465호; 본원에 참조로 포함됨)에 의존하였었다. 그러나, 생성되는 바이러스들은 병원성을 유지하거나, 또는 이전에 자연적으로 CMV에 의해 감염되었던 대상체에서 잠복 감염 또는 이차 감염을 확립하는 능력과 같은 유익한 특징을 상실하는 것 중 어느 하나였다.
본원에서 개시되는 것은 이러한 한계들을 극복한 HCMV 벡터 플랫폼 - HCMV-TR3 -이다. HCMV TR3은 분자 클론 HCMV-TR (문헌 [Murphy E et al., Proc Natl Acad Sci USA 100 14976-14981 (2003)]; 본원에 참조로 포함됨)의 변형된 버전이다. HCMV TR은 생체 내에서 잠복성 및 지속성을 확립하는 데에 있어서 다른 HCMV 스트레인에 비해 탁월하다. HCMV-TR은 또한 시험관 내에서도 다른 HCMV 임상 단리물에 비해 탁월한데, 그것이 다중 계대시 HCMV에서 통상적인 섬유모세포-적응을 나타내지 않기 때문이다. TR3를 그것이 간시클로비르-민감성이 되도록, 그것이 이전에 감염된 대상체를 재감염시킬 수 있게 되도록, 그리고 세균 인공 염색체 (BAC) 시스템으로부터의 CMV 벡터의 회수를 용이하게 하도록 변경하였다.
구체적으로, TR3로부터의 UL82 유전자 (pp71 단백질을 코딩함)의 결실은 확산-결핍 (즉 세포 대 세포 확산의 결함) 벡터의 생성을 초래한다. 그러나, pp71 발현이 결핍되어 있는 지금까지의 바이러스들은 시험관 내에서의 성장을 위해서는 보완을 필요로 하는 것으로 나타났었다 (문헌 [Bresnahan, W.A., and T.E. Shenk. Proc Natl Acad Sci USA 97:14506-11 (2000)]; 본원에 참조로 포함됨). UL82 비리온 단백질은 인간 시토메갈로바이러스-감염 세포에서 조기 발현 바이러스 유전자들의 발현을 활성화하는데, 이는 다시 바이러스가 활성인 pp71에 의해 야생형으로 복구될 위험성을 초래한다. 결과적으로, pp71이 결핍되어 있는 HCMV 벡터를 성장시킬 새로운 방법을 개발하고, 하기에 상세하게 기술하였다.
비-인간 영장류 모델은 또한 pp71-결실 HCMV-TR3이 이펙터 기억 T 세포 반응을 유도하고 유지하는 능력을 유지하는 반면, 통상적으로 섬유모세포를 통한 계대에 기인하는 바이러스 적응을 반복하는 HCMV-TR3의 향성-결핍 버전은 그렇지 않다는 것을 입증하고 있다.
또한, pp71-결실된 HCMV-TR3 벡터는 잠복 감염은 유지하나, 인간화된 마우스를 재활성화하는 능력은 결핍되어 있다.
또한, 이종유래 항원을 삽입하여 발현하는 데에 사용될 수 있는 내부 발현 부위들이 개시되어 있다. 이들은 다수의 이종유래 항원들을 포함하는 HCMV 벡터를 생성시키는 데에 사용될 수 있다.
실시예 2 - HCMV-TR은 잠복 감염의 확립에 있어서 다른 HCMV 스트레인에 비해 탁월하다
HCMV 잠복성 및 재활성화를 연구하는 것을 가능케 하는 인간화된 마우스 모델이 문헌 [Smith MS et al., Cell Host Microbe 8, 284-291 (2010)] (본원에 참조로 포함됨)에 기술되어 있다. 이와 같은 모델을 사용하여 HCMV-TR이 지속적인 감염을 확립하는 데에 있어서 다른 HCMV 스트레인 (AD169, 톨레도(Toledo))에 비해 탁월하다는 것을 입증하였다. 지속적인 감염은 이펙터 기억 T 세포의 유도에 중요하다. 지속적 감염을 생성시키는 능력은 이미 HCMV 백신의 임상 시험에 사용된 모든 스트레인 (AD169, 타우네(Towne) 및 톨레도)을 포함하여, 많은 HCMV 스트레인에서 돌연변이되어 있는 UL128-150 영역과 관계가 없다. AD169 스트레인에서의 UL131A의 복구는 잠복성을 확립하는 능력을 복구하지 못하지만, UL128-150이 결핍되어 있는 HCMV-TRΔ4 스트레인은 잠복성을 확립하는 능력을 유지한다 (도 1b). 이러한 이전의 임상 시험들이 이종유래 항원을 포함하는 HCMV와 연관되었던 것은 아님에 유의하라. 이들 스트레인의 유전자 맵을 도 1a에 나타내었다.
실시예 3 - HCMV-TR3은 간시클로비르에 대하여 민감성이며 US2-7 영역을 포함하는 반면, 원래의 HCMV-TR은 그렇지 않다
항바이러스 약물인 간시클로비르에 대하여 내성인 바이러스 단리물로부터 BAC 재조합조작에 의해 HCMV TR을 클로닝하였다 (문헌 [Smith IL et al., J Infect Dis 176, 69-77 (1997)]; 본원에 참조로 포함됨). HCMV 벡터에서 간시크로비르 내성은 바람직한 특질이 아닌데, 간시클로비르를 사용한 처리가 HCMV-기재 벡터에 의해 야기되는 CMV-연관 질환의 경우에 중요해질 것이기 때문이다. 간시클로비르 내성의 확인을 도 3에 나타내었다.
무손상 UL97 유전자를 HCMV TR에 삽입하여 (도 2) 간시클로비르-민감성 벡터를 생성시켰다. HCMV-TR의 분자 클론을 추가적으로 변형시켰다. 원래의 HCMV TR 클로닝 동안의 BAC 카세트의 삽입은 US2-7 영역의 결실을 초래하였다 (상기 문헌 [Murphy et al. 2003]). US2-7은 나중에 CMV-양성 개체의 재감염에 필수적인 영역인 것으로 확인되었다 (문헌 [Hansen SG et al., Science 328, 102-106 (2010)]; 본원에 참조로 포함됨). HCMV 스트레인 AD169의 US2-7 영역을 삽입하여 BAC 카세트를 변형시킨 변형된 HCMV-TR 버전을 생성시켰다. 이와 같은 변형은 원래의 HCMV TR 클론에서는 섬유모세포의 형질감염에 의해 바이러스가 재구성될 때 그 BAC 카세트가 제거될 수 없었기 때문에 이루어졌다 (문헌 [Lauron E et al., J Virol 88, 403-416 (2014)]; 본원에 참조로 포함됨). 이에 따라, 전체 게놈 서열분석에 의해 나타난 바와 같이, HCMV-TR3는 바이러스 재구성시 AD169의 US2-7 영역 및 US7과 US8 사이의 loxP 부위도 포함한다 (도 2).
실시예 4 - HCMV-TR3은 섬유모세포를 통한 다중 계대시 탁월한 게놈 안정성을 나타낸다
섬유모세포에서의 HCMV의 계대는 UL128-131A 영역 (문헌 [Dargan DJ et al., J Gen Virol 91, 1535-1546 (2010)]; 본원에 참조로 포함됨) 및 RL13 유전자 (문헌 [Stanton RJ et al. J Clin Invest 120, 3191-208; (2010)]; 본원에 참조로 포함됨)에 유해 (즉 불활성화) 돌연변이를 가지는 벡터의 우선적인 선택을 초래한다. 그러나, 섬유모세포를 통한 계대는 백신을 제조할 때 최고의 바이러스 수율을 초래한다. 도 4a는 놀랍게도 HCMV-TR3의 게놈이 섬유모세포에서의 20 계대 후에도 안정하게 유지된다는 것을 보여준다.
실시예 5 - 다른 HCMV 스트레인과 달리 UL128-131A의 존재가 세포-무함유 HCMV-TR3의 수율을 감소시키지 않는다
백신 제조의 경우, 세포 펠렛이 아닌 세포 상청액이 백신 벡터를 단리하기에 바람직하다. 대부분의 HCMV 스트레인에서, 유전자 UL131A, UL130 및 UL128이 무손상인 경우 섬유모세포로부터의 세포-무함유 바이러스 수율은 급격하게 감소된다 (문헌 [Wang D and Shenk T, J Virol 79, 10330-10338 (2005)]; 본원에 참조로 포함됨). 놀랍게도, HCMV-TR3에 있어서, UL131A-128의 제거는 세포-무함유 대 세포-회합 바이러스의 비에 영향을 주지 않았다 (도 5).
실시예 6 - HCMV-TR3는 원숭이에서 이펙터 기억 T 세포를 유도하는 반면 UL128-131 영역이 결핍되어 있는 HCMV 돌연변이는 그렇게 할 수 없다
비-인간 영장류 (NHP; 도 6a)에서, SIV의 Gag-항원을 발현하는 HCMV-TR3은 Gag에 대한 이펙터 기억 T 세포 반응을 유도할 수 있다. 중요한 것은 이와 같은 이펙터 기억 T 세포 반응이 오랜 시간 동안 유지된다는 것이다 (도 11). 반면, 시험관 내에서의 연속 계대에 의해 약독화된 HCMV 스트레인에서 빈번하게 돌연변이되는 유전자 영역인 유전자 UL128-131이 결핍되어 있는 HCMV-TR3은 그렇게 할 수 없다 (도 6b). 이는 비-인간 영장류 모델에서 이종유래 항원에 대한 면역 반응을 유도하는 HCMV 벡터의 처음으로 알려진 입증이기도 하다. 이와 같은 게놈 영역에서의 추가적인 결실은 UL128 및 UL130이 결핍되어 있는 바이러스가 모체 벡터와 유사하게 생체 내에서 이종유래 항원에 대한 면역 반응을 도출할 수 있다는 것을 입증하고 있다 (도 12). 이에 따라, UL131A가 HCMV에 의한 감염에 필수적이라는 결론을 내렸다.
실시예 7 - DAXX siRNA를 사용한 비보완 pp71-결실 HCMV-TR3의 생성. 보완 또는 FKBP-융합 없이 약독화된 바이러스를 성장시키는 방법
필수 유전자, 또는 시험관 내에서의 최적의 복제에 필요한 유전자가 결핍되어 있는 HCMV의 제조에 있어서의 주된 한계는 보완 - 즉 생산자 세포주에서의 결실된 유전자의 외인성 발현 -의 필요성이다. 생산자 세포주는 특히 GMP 백신 제조 맥락에서는 제조하고 유지하기가 어려운 것으로 잘 알려져 있다.
보완에 사용되는 한 가지 접근법은 필수 유전자를 분해 도메인 (예컨대 FKBP)에 융합시키는 것으로써, WO2013/036465호 (본원에 참조로 포함됨)에 기술되어 있는 전략이다. FKBP-융합이 생체 내에서 복제 결핍인 비-지속성 백신의 제조에 유용할 수 있기는 하지만, 본원에서 기술되는 돌연변이 HCMV의 경우, 분해 도메인이 돌연변이되고 그에 따라 약독화가 상실됨으로써 HCMV가 숙주에서 숙주로 확산할 수 있게 될 위험성이 존재한다.
본원에서 개시되는 것은 예를 들면 siRNA를 사용하여 항바이러스 숙주 세포 인자를 침묵화하는 것을 포함하는 접근법이다. 생체 내에서 그것이 약독화 유지되는 경우에도 돌연변이 HCMV가 시험관 내에서 성장될 수 있기 때문에, 결과는 보완을 필요로 하지 않는 세포주이다. 이와 같은 과정의 예를 도 7a에 예시하였다. 상기한 바와 같이, 인단백질 71 (pp71)을 코딩하는 UL82 유전자가 결핍되어 있는 HCMV-TR3는 섬유모세포에서 성장할 수 없다. 그러나, 섬유모세포에서 발현되는 siRNA에 의해 항바이러스 단백질 DAXX의 발현이 침묵화되는 경우, HCMV-TR3ΔUL82는 높은 수율로 성장될 수 있다 (도 7b 및 도 13).
실시예 8 - UL82 (pp71)가 결핍되어 있는 HCMV-TR3은 생체 내에서 지속성을 유지하지만 잠복으로부터 재활성화되는 그의 능력은 결핍된다
인간 시토메갈로바이러스 (HCMV)는 시간별 발현 바이러스 유전자를 통하여 조절되는 숙주 세포에서의 잠복 감염을 확립한다. HCMV pp71은 세포 단백질 Daxx (사멸 도메인 연관 단백질)의 숙주 고유 면역 분해에 대항하는 외피 단백질이다 (문헌 [Penkert, RR, and RF Kalejta, Future Virol 7, 855-869 (2012)]; 본원에 참조로 포함됨). pp71에 의한 Daxx의 분해는 최적의 조기 발현 유전자 발현 및 용해성 복제(lytic replication)에 필수적이다. 시험관 내 데이터는 HCMV가 잠복성의 확립 동안에는 감염된 세포의 세포질에서의 pp71의 봉쇄에 의해 Daxx의 pp71-매개 분해를 방지한다는 것을 암시하고 있다. 그러나, HCMV 지속성, 잠복성의 유지 및 재활성화에서의 pp71의 생체 내 역할에 대해서는 알려져 있지 않다. 면역 결핍 마우스 (HU-NSG)에 이식된 인간 조혈 줄기 세포 (HSC)의 HCMV 감염이 G-CSF 처리 후 재활성화될 수 있는 바이러스 잠복을 초래한다는 것은 이미 밝혀진 바 있다. 이와 같은 모델이 중요하기는 하지만, HU NSG 마우스에는 성숙한 인간 T-세포가 결핍되어 있다. 반면, 인간 태아 간 및 흉선과 함께 HSC가 이식된 NSG 마우스 (BLT 마우스)는 성숙한 CD4 및 CD8 T-세포를 포함하여 기능성인 인간 면역 시스템에 필요한 모든 인간 조혈 세포 계통을 발생시켰다. 이와 같은 새로운 인간화 마우스 모델에서, HCMV가 HU-NSG 마우스와 유사한 잠복성 및 재활성화를 확립한다는 것이 입증되었다. 잠복 감염된 마우스는 또한 인간 IgG는 물론, HCMV-특이적 T-세포 반응도 생성시킨다. 중요한 것은 조건부 발현 pp71 (TR UL82-FKBP) 또는 pp71 녹아웃 (TR(델타)UL82)을 사용한 BLT 마우스의 감염이 감염의 확립은 초래하나, 재활성화하는 데에는 실패하였다는 것이다. 이러한 데이터는 pp71이 HCMV 재활성화에서 중요한 역할을 한다는 것, 및 복제 결핍 바이러스가 T-세포 반응을 생성시킬 수 있다는 것을 표시한다. 도 1b에 나타낸 바와 같이, 시험관 내에서 복제하는 능력은 바이러스가 잠복성을 확립할 수 있는지 여부에 대한 우수한 지표는 아니다. 예를 들면, 도 1b에 나타낸 바와 같이, AD169는 시험관 내에서 잘 복제되지만, 잠복성은 확립할 수 없다. 그러나, DAXX siRNA 발현 MRC-5 세포에서 성장되는 HCMV-TR3ΔUL82는 인간화된 마우스에서 잠복성을 확립하지만, 재활성화되거나 확산되지는 못한다 (도 8). 유사한 결과가 HCMV-TR3ΔUL82 및 HCMV-TR3ΔUL82ΔUL128-130에 대하여 NSG 마우스에서 수득되었다 (도 14).
실시예 9 - HIVgag를 발현하는 pp71-결실 HCMV-TR3는 생체 내에서 HIVgag 특이적 이펙터 기억 T 세포를 유도하는 능력을 유지한다
커다란 그의 게놈으로 인하여, HCMV는 다수의 이종유래 항원을 바이러스 벡터에 삽입할 기회를 제공한다. HCMV에 의한 다수 이종유래 항원의 발현은 벡터 기능에 영향을 주지 않으면서 외래 서열을 삽입하는 데에 사용될 수 있는 내인성 유전자의 확인을 필요로 한다. 이미, 트랜스포손 분석상, 시험관 내의 HCMV 게놈에서 모든 비필수 유전자들이 확인되어 있다 (문헌 [Yu D et al., Proc Natl Acad Sci USA 100, 12396-12401 (2003)]; 본원에 참조로 포함됨).
그러나, 이것이 시험관 내 비-필수 유전자가 생체 내에서 비-필수가 될 것이라는 것, 그리고 나아가 이종유래 항원을 코딩하는 유전자를 사용한 바이러스 유전자의 대체가 대체된 유전자의 프로모터에 의해 이종유래 항원의 발현이 추진될 때 면역 반응을 유도할지 여부에 관한 예측을 제공하는 것은 아니다. 도 9 및 도 15는 HIVgag를 사용한 UL82(pp71)의 대체가 생체 내에서 이펙터 기억 유형 T 세포 면역 반응을 도출하여 유지한다는 것을 보여준다.
이종유래 항원을 사용한 대체를 위한 추가적인 부위에는 HCMV UL7, UL78 및 US13이 포함된다. 이들 각각이 이미 항원 (SIVenv)에 의한 pp71-ORF의 대체를 보유하는 벡터 중 이종유래 항원 (SIVpol)으로 대체될 경우, 매번 면역 반응이 생성되었다. 결과를 도 10a에 요약하였다. 도 10b는 HCMV에서의 HIVgag를 사용한 UL7, UL45 및 UL78의 대체가 시험관 내에서 HIVgag 발현을 초래한다는 것을 보여준다.
실시예 10 - 조건 보완하에서의 성장 및 제조 동안의 pp71 결실 HCMV-TR3의 안정성
이전의 연구는 섬유모세포에서 성장될 경우, HCMV의 임상 단리물이 시험관 내에서의 빠른 적응에 적용된다는 것을 입증하고 있다. 특히, 조직 배양에서의 적은 수의 계대 후에도 RL13에서의 조기 정지 코돈으로 이어지는 프레임시프트 돌연변이의 생성, 그리고 오량체 복합 단백질 (UL128, UL130 및 UL131A) 1종 이상의 발현 상실이 발생할 수 있다 (문헌 [Stanton RJ et al. J Clin Invest 120(9), 3191-3208 (2010)]; 본원에 참조로 포함됨). BAC에서의 완전한 인간 시토메갈로바이러스 게놈의 재구성은 RL13이 복제의 강력한 억제인자라는 것을 밝혔다 (상동). 결과적으로, 임상 연구에 이미 사용된 모든 HCMV 스트레인 (AD169, 타우네, 톨레도)은 다중 재배열 및 결실을 나타낸다 (문헌 [Murphy, ED et al. Proc Natl Acad Sci U.S.A. 100(25), 14976-14981 (2003)]; 본원에 참조로 포함됨). 이러한 섬유모세포-적응은 AD169에서 관찰된 바와 같은 UL131A의 결실을 초래함으로써, 바이러스가 생체 내에서 비-감염성이 되도록 할 수 있다. DAXX siRNA로 처리된 섬유모세포 세포에서 성장된 UL82-결실 HCMV-TR3/HIVgag가 다중 계대시 유사하게 불안정성을 나타내게 되는지 여부를 확인하기 위하여, 차세대 서열분석 (NGS)에 의해 바이러스 게놈을 분석하였다.
구체적으로, 재조합 세균 인공 염색체 DNA를 섬유모세포에의 도입 전에 서열분석하고, 섬유모세포에서의 재구성시 계대 5 및 계대 9에서 바이러스 DNA를 단리하였다. 20 % 수크로스 완충제를 통한 바이러스 정제 후, 히르트 추출(Hirt extraction) (문헌 [Hirt B. J Mol Biol. 26(2):365-369 (1967)]; 본원에 참조로 포함됨)에 의해 감염된 인간 섬유모세포의 상청액으로부터 게놈 DNA를 단리하였다. TruSeq DNA 샘플 제조 키트를 사용하여 DNA 라이브러리를 생성시키고, 알려져 있는 프라이머 결합 부위를 가지는 어댑터들을 DNA 단편의 각 말단에 결찰시켰다. 일루미나(Illumina) MiSeq NGS 서열분석기에서 MiSeq 시약 키트 v2를 사용하여 300 주기 동안, 미지 DNA의 각 말단에서 150 bp를 분석하는 쌍별 말단 서열분석으로 수행하였다. 결과적인 서열 해독치를 지니오우스(Geneious) 8.1.4로 불러, 0.1 %의 더 높은 오차 가능성을 가지는 모든 염기 쌍이 결실되는 것을 의미하는 0.001의 최저 가능 오차 가능성 한계로 트리밍하였다(trimmed). 250.000 내지 1.000.000개의 해독치를 사용하여 신규 서열 어셈블리를 수행함으로써, 언바이어스드 방식으로 DNA 서열을 확인하였다. 예측 서열과 비교하였을 때, 주요 삽입, 결실 또는 게놈 재배열이 관찰되지 않았다. 다음에, 참조로서 신규 서열을 사용하여 모든 해독치의 참조-안내 어셈블리를 수행함으로써, 전체의 올바른 주요 서열을 확인하였다. 평균 최소 커버리지(coverage)는 > 150배이었다.
도 16은 최종 서열의 정렬을 보여준다. 자기-절단 BAC 카세트에 코딩되어 있는 오픈 리딩 프레임 (ORF)을 백색의 화살표로 도시하였으며, 바이러스 ORF를 회색의 화살표로 도시하였다. 황색의 화살표는 UL82 ORF를 대체하는 HIVgag ORF를 도시한다. 회색의 타원은 내부 및 말단 반복체를 도시한다. 비-코딩 영역은 흑색 막대로 보이는 코딩 영역의 중단으로 나타내었다. 예상대로, BAC 카세트는 조직 배양에서의 바이러스 재구성시 절단되었다. 그러나, 대부분 서열의 모든 다른 뉴클레오티드들은 예측 서열 (컨센서스)과 동일하였다. 중요한 것은 9 계대를 통하여서도 ORF에 있어서 어떠한 아미노산의 변화도 관찰되지 않았다는 것이다. 여기에는 UL128-131A 유전자, RL13은 물론, AD169-유래 유전자 UL97 및 US2-7을 코딩하는 ORF가 포함된다. 이러한 관찰은 DAXX siRNA 존재하에 섬유모세포에서의 다중 계대에도 불구한 UL82-결실 HCMV-TR3의 놀라운 안정성을 암시하고 있다.
중요한 것은 UL82 프로모터에 의해 발현되는 HIVgag를 코딩하는 ORF에서의 변화가 없었다는 것이다. 이는 독립적으로 UL82(pp71)-결실 HCMV-TR3의 재구성 후 5, 6 및 7 계대에서의 HIVgag 삽입물의 면역블럿 및 생거-서열분석에 의해 확인되었다. 도 17a는 표시된 바이러스에 의해 감염된 섬유모세포로부터의 용해물의 면역블럿을 나타낸다. 용해물을 SDS-PAGE에 의해 분리한 후, 나일론 멤브레인상으로 전달하고, pp71, HIVgag (p24), 및 바이러스 단백질 pp28 및 세포 단백질 액틴에 대하여 특이적인 항체들과 반응시켰다. 예상대로, pp71은 모체 TR3 바이러스에는 존재하였으나, HIVgag에 의한 UL82의 대체로 인하여 HIVgag-발현 벡터에는 존재하지 않았다. 중요한 것은 HIVgag가 각 계대시 안정하게 발현되었다는 것이다. 도 17b는 HIVgag 유전자를 포괄하며 표시된 계대에 바이러스 DNA로부터 수득된 PCR-단편의 서열 분석을 기준으로 한 정렬을 나타낸다. 뉴클레오티드 변화는 관찰되지 않았다.
내인성 UL82 프로모터에 의해 발현되는 HIVgag의 놀랍도록 안정한 발현과 달리, 이종유래 프로모터에 의한 이종유래 항원의 발현은 보통 다중 계대시 불안정하다. 예를 들면, RhCMV 68-1.2 벡터에서 이종유래 EF1α 프로모터에 의해 발현되는 SIVgag는 점 돌연변이로 인하여 코딩 영역의 조기 붕괴를 나타내었다. 도 18은 EF1α 프로모터를 사용하여 SIVgag를 발현하는 RhCMV 68-1.2의 클론으로부터 유래하는 UL36-결실 RhCMV 벡터의 차세대 서열 분석으로부터의 참조 서열과 비교한 단일 뉴클레오티드 다형 (SNP)의 빈도를 나타낸다. 대략 38 %의 게놈이 SIVgag 서열에서 조기 정지 코돈을 나타내었다.
SEQUENCE LISTING
<110> Frueh, Klaus
Nelson, Jay
Caposio, Patrizia
Picker, Louis
Hansen, Scott
<120> HUMAN CYTOMEGALOVIRUS COMPRISING EXOGENOUS ANTIGENS
<130> 1996-1/065335-202240/P070PCT
<150> 62/025,348
<151> 2014-07-17
<160> 3
<170> PatentIn version 3.5
<210> 1
<211> 244443
<212> DNA
<213> Human cytomegalovirus
<400> 1
ggtggtgttg cctgcggcgg ggacgggggt tgcgctggga tcgggggtgg cgccggggac 60
ggggggcttt cgcagcgggg aacacacacc gcctatttaa cctccacccg ctacaacaca 120
cacatgccgc acaatcatgc cagccacaga cacaaacagc acccacacca cgccgcttca 180
cccagaggac caacacacgt tacccgtaca ccacagtaac acacaaccgc aagtccaaac 240
ctcggacaaa cgcgccgccg aagaccaccg cacgcagatg gagctcgacg ccgcggacta 300
cgctgcttgc tcacaggccc gccaacacct ctacggtcaa acacaacccc aactacacgc 360
ataccccaac gccaacccac aggaaagcgc tcattttttc acagagaatc aacatcaagt 420
cacgcatcta cttcacaaca ttggcgaggg cgcagcgctc ggctaccccg tcccccgcgc 480
ggaaatccgc cgtggcggtg gcgactgggc cgacagcgca agcgacttcg acgccgactg 540
ctggtgcatg tggggacgct tcggaaccat gggccgccaa cctgtcgtga ccttactgtt 600
ggcgcgccaa cgcgacggcc tcgctgactg gaacgtcgta cgctgccgcg gcacaggctt 660
tcgcgcacac gattccgagg acggcgtctc tgtctggcgt cagcacctgg tttttttact 720
cggaggccac ggccgccgtg tacagttaga acgtccatcc gcgggagaag cccaagctcg 780
aggcctattg ccacgcatcc ggatcacccc cctctccaca tctccacgcc caaaaccacc 840
ccagcccacc acatccaccg cctcgcaccc acatgctacg gctcggccag atcacacgct 900
ctttcctgtc ccttccacac cctcaaccac ggttcacaat ccccgaaact acgccgtcca 960
acttcacgcc gaaacgaccc gcacatggcg ctgggcacga cgcggtgaac gtggcgcgtg 1020
gatgccggcc gagacattta cgtgtcccaa ggataaacgt ccctggtaga cggggtaggg 1080
ggatctacca gcccagggct cgcgtatttc gccgccacgc tgcttcaccg atatccaata 1140
aacccatccc ctcgccacga cgtctccgcg tatctttgta gcctcaggaa tccgtcccca 1200
cgtccaccca tcccgagcac tccacacgct ataacagacc acggacacgg caaatgcatg 1260
caaacttctc atttattgtg tctactactc tgtgttgcta cagggggtga aggcaaagaa 1320
aaaaaaaagg aacaaaataa tagattagca gaaggaataa tccgtgcgac cgagcttgtg 1380
cttcttttct tataaggagg caaatatact agggaaaaca taagaatagg aagaaaccga 1440
ggtttgggag aaaagctgag ataaaatagc gcattttcca tacagaggtt gttgtttttg 1500
tggatcctaa gaggtttcaa gtgcgaatct taaagttctc acgagaatat tgtcttcaag 1560
aatcgacaac tgtggtccaa gatttttttt tggtcttttt aggttctgcg agggacatca 1620
cgatggatcg ttgcgatgaa gtcacgcgta cgcctctggt gtggcgcggt gtcgtgacag 1680
gagagtgtgt tttcagtgca gagctgtctt gattcctata tccgagtatc tgttttctcg 1740
taaggacggt aatcttcttt ggtgtaagta catctaaaag ctgcaaacta tattttaagg 1800
gctgtctcta ggtgtacttt gatgctggag tttttcgctg tgttgatgtg aataaatcta 1860
ctactactat tatatgcaga aagagtgatt atgccgagac aagattgcat tggctgaact 1920
gtttcaaaaa cgcctacact ctacttatcc gtaaacctaa ggtaatacta tgtgtaagtt 1980
gttttttttt tctttttgta gtaaaatggt gatacgtgca attaaaactg tattccatgt 2040
ttccatcctt tcatttcaac tttaaaggcg gctttgagag cgaagaagtg cgaggataaa 2100
aatggatgac tccttcgtgt ccagggagtc gactactgca acgctgattg attaaaagat 2160
ggtctccgat gatgatgttg ttattgatcg aatcatggtg cagaacggcg acggagagga 2220
gcgtgtccgc cgccgggaag gtggtctctt tctcttttct tttttcaaga aatcttccat 2280
gtgtttatcg tagtgatcga aatcgactga tctcgggttc tttttgttgg tttcttttcg 2340
gttaatcatg tattgttttc tttttttaca gaaagatact tttttcatga gcaattcctc 2400
gcccggcgcc ggcatgccga ggtggggcca ctgcgatcag cggcatgccg acgccgaccc 2460
ggggatcttg gattcaccgt tttctctctt ctctctctac atacagaccg ggtggcagga 2520
gcggtaagga atcatcgtcg tctttcattc ttcgatgatt atggtaatac taaatcttat 2580
ctaggagcat atacatctaa gattggagta ctagtagtcg tttgtggttt ctattttttt 2640
ttatatttat ctatgacagt ttttctgttt ttcgttttga taataatata ataaaaactc 2700
atggacgtga aatctggctt ggttgtggtg atttcattct cattattgtt gttttctttc 2760
cgtcttgcgg atgaagatgt tgcgatgcgg ttgttgttgg tgttgctata caccgagaga 2820
gatgatcttt ttgttcttct ggttcatttc ctatgattgt ttggctgctg accgacgcgt 2880
caggatgtgc agggcatgcg gggaatcagg accggacacg ggataatttc atctacctat 2940
acggagatcg cggtcctcgc catgaggatc gcgacaggcg cgtcgagggg gcaggaacac 3000
ccttgcggat tgacattctt ggtggtgttt cgttgttgtc ggtagttgtt gttgacgatg 3060
aggataaata aaaatgacct tgtttttgtt ctgttttctc ttgttgggaa tcgtcgactt 3120
tgaattcttc gagttatcgg aaagctgagg tacccaaatg tctgtagctt ttttcttttt 3180
accctcttgt ttatcatctg cgattcgtgg taggtaggag agggaaatga taatccgaga 3240
ttaaggaaag gagaagataa aataaaaaaa aaataaaaca gaagccgacc ggccgccgac 3300
ccgttcccca ggaccagcct acgaggaacg gataacgcgg tggcgacggc agcggtggtg 3360
gcgctggggg tggcggtagt ggtactgctg atggtagtcg ggacggagga gagacgatgc 3420
atacatacac gcgtgcatgc tgcatgggtg gatggtccga ccgggagacg cggaagagaa 3480
actcacataa aaaggtgaca aaaagagcgg ttgaaaaaag aaaacgagat tcgaccagac 3540
agaagaggag gaccggggct tggcgaccct tccacgactg ctgttgtcat ctcggctcct 3600
ccgtcttctc ccggccacgg gcggctaagt caccgccgtt ctccccatcc gtccgagcgc 3660
cgaccgacca gccggccgat tcgcccgccg gggcttctgg agaacgccgg ggcagcagcg 3720
atctggagaa gccgctaaac ccctgcgttt ttatatggta gctctgccga gcgcgggctg 3780
acgcgttaag taagcggaaa gacgtgtgtg acgaaaaggg gtcccatggt atttcacgtg 3840
acgatgagga gatgcggttt ggagcacata cggtttagaa aaagggagtt gtcgtgacaa 3900
gggctgaggg acctctgtct ccatgtgtgt ataaaaagca aggcacgttc ataatgtaaa 3960
aaagaacacg ttgtaaacaa gctattgctg tatcattcgg ctgactatgc ttcattcgga 4020
ctgattttct tttcctaacg gcgtaactta aagtgattaa cgtatgatat ttgttcccca 4080
gagttatact atagtcatca tcctaaaatt cagatataaa tgaacacatg tcgtatgaga 4140
ttattaagaa accgaaacca cccatagttc accatcctct tcatcattca gccgatgacc 4200
cactccgtac aacgactcag tctgcttcgt catattgcaa agcacaagcg acgtatgtga 4260
acaacttgaa acacagactg tgttattaat gaccgttgta ccattactag tcacattgca 4320
taaagatcct ccgccgtcgt cccatctttt ccactcggtg gaaaaccggt cgctatcatc 4380
aactatggtg agattttcac cctgcgtggt attcagtttc ttcatattca taccttggat 4440
tccattatta aaccccaata ttaagcacgt tattagtacc cccccccccc accaaggaat 4500
gtgactggac cggttcctag cagctctggg agccatgttc aggttgaacc acagctacag 4560
cgaaaccgag tccagtgacc ggtaaccacg tccagcccct gcgtatgtac cagtccaagc 4620
acgtccggtc attgttctac acaggaaatc taactaggtc aacgtaattt tattccaccg 4680
ttacgcagaa tactaacaaa aaactacaca aatgtaacgg attacacata atttattacg 4740
tgaaaactgt aagaaagcca attcaccaag cgatacattt atttgacttc caagtgccac 4800
acatcaccac tatattcatc catgttttca ccgaaccaac gagacagatc gaagaagcca 4860
gaatcttccg actttaaatt acataaatcc aacgtattat gaccacagct cgacacacaa 4920
atagttgcgt tactattcac agtggcatta cctatacccg taacgttgca caaccactga 4980
tcaccattgt caccaaaaac ggttttccac ttagttgtca acggatcttt cctatgcgta 5040
atggtaaaat tactaccagt cgtcgctttt agctcattac gagtattatc cgcatccaca 5100
tatatcaacg tcatagctag gcacgctata agtacccccc ccccacaatg gaatgttgcc 5160
aaaccggttc tttcccgtta tagccatagc gttcccaggc aaaagcaaac gccgaaccta 5220
atgcagtaaa aagcgcttgc agccagaacc agcttatgta ccagccacga taacatccgg 5280
tcactgtttc cacaggaaac cctaccaggg tagagccccg cttgtttttt cctgtctatc 5340
ttgtttagca actcgtaaac tgtcagtcta gccacgtccg tttagatcaa aagtcacgta 5400
tactgcgacg ttgtttccac ccgtttcccc gtcccgccgt ttccgaacaa cccacccggg 5460
ttcagacaac cgaccaccaa cagaaatata cacacagacc accgggagtt cagttaaaga 5520
tttcatcagg tttattttgg ctgctgctag tcttttgctt cttagaaaaa aaatacccat 5580
atagagaaat aatgatagtt tgacaacaca tatggcaggg atttcttctt catcaataag 5640
atatgcaatt cccccaggga gagactttca acaattgaat ttacaaaaac aaaattacat 5700
caggagaaag agaggataca ttaataaata tattatatct ggtgtatata ctgaatgctg 5760
ctggttcata aggtaacgat gctacttttt ttaattccaa gatggttttt ctttgttagt 5820
cttttgttga cttgctggtt cctaaaagtt ctcaaaaacg attgtgtgaa gattttatga 5880
cgttggttga ctagttcatg agattctgct gtacgtgtga tggttattcg ctggttcgtt 5940
ctaagatgag tatcgtactg tgtctgcgat ggtcgtctct tactggcatt ctctcggctg 6000
cctcttgctt tcatgattga aaaggaaaaa aggactccga gggcgcggtc atcttttact 6060
tttcggtttt ttcgttggcg ggtcagaggt agtcagatca tgagactgtc gtggtcgatg 6120
aaactgtgtc tgctcaagtg acgtccattt cttgtacgga gaaaaaagtc atcgggataa 6180
ataaggctat acaaggcgtt gtcaagcgtg cggctctaaa caaattaagc gatacaaaat 6240
tacagtaata cgaataataa gttaccccct ccccctgtgg tccccccgag acgagagcca 6300
cccatcgtgt actctcgcac cacccacgac cacagaggga gacgggacga agagacgacg 6360
cagagcgcca tctccttctg gaggccggcg gcgttaactg ctacagctgc ggcggcgaca 6420
acagctgcga tttgtcggcc gacatgccga tggtatgggc ggcggcggca gtggccgcgg 6480
cagcggggag gagaggagag agaagaggag cggggcgtcc gaaggcgagg atggcatggt 6540
ctcgccggag cgcccggctt ttatggaacg ctcgcgtccg gtcgggcagc gcccacagga 6600
agatgagtca aaacttttaa accatcctga gacccgagta gcggtttaca ggccgcacgc 6660
cagtcttagc taaaaacagc ggacagtccc acgctgtttc tgttgtggct ctctccagtt 6720
tcctcatcgc cgtcccgatc tccgtcgtca tcggaagaat accacccgct ctcatgcggc 6780
agtcgatcga cctcgacgaa cgagacgcgg cgacgcctct ctacggccga ctggttgtgg 6840
tggtgaaaga agagcaccag caatcccagg aggagcaaca agccctcaca tgtccaggag 6900
gtcggggaga gggcctgtcg gagatggccg tgaggcatca cgtacggcag ctgaggagaa 6960
acggagaaga aaggaaaatt accgtcaggg gccggggttc ttattagaga aacagcacgt 7020
aggtcaggat ccagatgcta atggcaatca tgatgacgat gatcatgcag gccaagacgc 7080
ggcgcaccaa tgccgaatcc aatagccgcc gtgcctccgg ttggtggccg gcggcatcta 7140
gagacatgat ttggggggga ccggcggcgc gaaaagacag ggagatggac agtgtcacgg 7200
tgttttgtta tgattaggac atggggaccg gaagccgaga cagagtacta cagggtgttg 7260
aagggtaacg tgagggagat catgtcatgg gcgggctgaa gaccgtgcgg ggaggattgg 7320
cgtgtgcggt gcttgtggaa cacggtgttt taatatgtat ccgcgtgtaa tgcacgcggt 7380
gtgcttttta gcactcggct tgataagcta cgtggccgtt tgcgccgaaa acacggttac 7440
caccaactgt ctcgtgaaaa cagaaaatac ccacctaaca tgtaagtgca atccgaatag 7500
cacatctacc aatggcagca agtgccacgc gatgtgcaaa tgccgggtca cagaacccat 7560
taccatgcta ggcgcatact cggcctgggg cgcgggctcg ttcgtggcca cgctgatagt 7620
cctgctggtg gtcttcttcg taatttacgc gcgcgaggag gagaaaaaca acacgggcac 7680
cgaggtagat caatgtctgg cctatcggag cctgacacgc aaaaagctgg aacaacacgc 7740
ggctaaaaag cagaacatct acgaacggat tccataccgg ccctccagac agaacgacaa 7800
ctccccgttg atcgaaccga cgggcacaga cgacgaagag gacgaggacg acgacgtctg 7860
ataaggaagg cgagaacgtg ttttgcacca tgcagaccta cagcaccccc ctcacgcttg 7920
tcatagtcac gtcgctgttt ttgttcacaa ctcagggaaa tttatcgaac gccgtcgaac 7980
caaccaaaaa acccctaaag ctcgccaact accgtgccac ctgcgaggac cgtacacgca 8040
cgctggttac caggcttaac actagtcatc acagcgtagt ctggcaacgt tatgatatct 8100
acagcagata catgcgtcgt atgccgccac tttgcatcat tacagacgcc tataaagaaa 8160
ccacgcatca gggtggcgca actttcacgt gcacgcgcca aaatctcacg ctgtacaatc 8220
ttacgattaa agatacggga gtctatcttc tacaggatca gtgtaccggc gatgtcgagg 8280
ctttctacct catcatccac ccacgtagct tctgccgagc tttggaaacg cgtcgatgct 8340
tttatccggg accagggaga gttgtggtta cggattccca agaggcagac cgagcaatta 8400
tctcggattt aaaacgccag tggtccggcc tctcactcca ttgcgcctgg gtttcgggac 8460
tgatgatctt tgttggcgca ctggtcatct gcttcctgcg atcacaacga atcggggaac 8520
aggacgctga acagctgcgg acggacctgg atacggaacc tttgttgttg acggtggacg 8580
gagatttgga ataaaagatg cgcgtcaacc gtcaaagacg caacaacctt acgtaccgac 8640
aaacggtata tgtaattctg accttctaca ttgtacatag gggcatatgt aacagcaccg 8700
ataccaacaa ttctacatct acttcaaata gcaccgtctc tgatactaat gtatattcta 8760
ctccaaatcc tcccagtgta tcttctacaa ctcttgatac atctaccgac tcacagatat 8820
caatcgcctc aaacaccata tccagcacta caaatacatt gaccgcatat tctataacta 8880
cgttaaacac ctcgacttca tcttcaactc ttactgctgt ctctagtacc catacaagat 8940
cctcaatact ctccaacaac gcatcataca ccacatcatt ggataataca actacagaca 9000
taacgtccag cgaaagttca atcaacgtgt cgacagttta caacaccacg tacattcctg 9060
taacatcgct tgctattaat tgtactgcta caattaacgg aacaaataat tctagttcaa 9120
aaacttgcca acaagacatt gaaacaatac ctgtgaaatc aactccacta acggcagaag 9180
aaggaacaaa tattacaata catggcaatg acacgtggga ttgtcccgac gtggtttggt 9240
atcgacatta taattggtct acacatggac accacattta tcccaataca cattacaaaa 9300
ctttgataca tcgacgcaag atcctaacgt cacatcctat atgttattct gatcgctcat 9360
cacctaccgc gtatcatgat ctatgccgtt catgtaataa aacagaacta cgcctttacg 9420
atttaaacac caccaattct ggtagatata gccgacggtg ttacaaacag taccatcacc 9480
agggaccaca cgaggatgaa aatttcggac taactgtaaa tcccaggaac aacactgaca 9540
attataccat cccagtatgt cccagatacg tagaaacaca atcacaggaa gatgaacaag 9600
acgacgatta tacactaagc actaccataa ataataatct tatgcgcaaa acaggtcact 9660
atgacatctc acatggcacg cacactacat gggctcttat actaatttgc atagcctgca 9720
tgcttctttt ttttgttcga cgagccctca ataaaaaata tcgtccacta cgagatgata 9780
ttagtgaatc tagccttgtt gtgcaatatc atcctgaaca tgaagactaa cgtttccgga 9840
catgcaacac ataaaattaa gtaacatatc taccatgaag tacagcaaat atctactaat 9900
gtctatccat ccaacagtga taccatgcac tggcatcttg cgattacatg gacggtaatc 9960
atatccacgt tttcggaatg ttgtaaccaa acttgtccgt gttcctgcgt ttgtgtcaat 10020
tctacaacag tcaacatatc cacaaatgaa acaacgtcta aagccatcac tccaactgct 10080
acgacaaata ccgcaaaaac aacgtcaagc cttgttatta ctacaccgtc atcagtaacg 10140
attagcaaag ccgtgtctac tgcagcttca tcaaccatac tatctcaaac caatcgcagt 10200
catacaagta atgtcatcac aaccccaaaa acgcggtttg aatataatat cacgggatat 10260
gttggccaag aagtgacttt caacttcagt ggatcatttt ggagctacat tgaatggttc 10320
cggtacagtt ctccaggctg gctttattcc tcggaaccaa tatgcaccgt taccaacagt 10380
tatcatcata ctttccctcg tggtacctta tgtttcgatt gtaacatgac aaaatttgtt 10440
atttacgatc taacgttaaa cgattctgga aagtacgttg tcaagagaac acgtcatgac 10500
aatcaatacg aagaagcatg ctacaatctc acggtaattt atgccaacac gacagccata 10560
gttaccaaca ggacgtgtga tagaagacaa acaaaaaata cagacactac taaccatgga 10620
atcgggaaac atattattga aactattaaa aaagccaaca ttcccctggg gattcatgct 10680
gtgtgggcgg gcatagtggt atcagtggca ctcatagcac tatatatggg taaccgtcgt 10740
aggcccagga aaccgcgtta taccagactt cctaaatacg acccggatga gttttggact 10800
aaaacctgat atgcacatca ataaactttt ttgtatcttt agttattaat gtctgtgtgt 10860
ttattcagaa taactcattt ataatataag acggaatatt catatacatt aaaaacatgg 10920
gtgtacaata taacactaaa ctgttattag ccgtattagc aattatccca gctggcattc 10980
tagtacaggc aatttcacat gagcaaaaaa catcctaccg gcaacttttg ctgcaaagtg 11040
aacgtgtgca aatacccatc acaacagtcg agggagatac aatttgcttt aacgttagta 11100
acaacccctg caacttttcc agttattgga atcacaataa ttgtgaactt tgcggttgga 11160
caccgttttt ctttgaatat gctggatata ctgaaaacac gtcgtgtcac ccacgattta 11220
cctgtattca tgatactaaa ggtctaaaac tatacaatgt aaccatgaat gactcgggaa 11280
tttatacaca acacgtttat cactgtgata ttccatgtaa catcagcgat gatcgtaaat 11340
ataacgtaga tgacattgat aactgcaacg ctactataaa tgtaaccgac tatattatta 11400
ccgtgttgtc ttcacgttat tctaaacgca ccgattacca cgtagatact tacattggtt 11460
atgcaaccac tgtggtgaca atagtattta tctgtgtttt aacttgcatt aacgtctcag 11520
caactctaag gcacagacta cgaactagaa acaacgttaa cagcataacg tgattacaaa 11580
gtatcaacgc tagtttatcc aagagaaact ttcatgaagg atcgcaataa agcattgcta 11640
tgtatcatct ttattttaat catgtacctc atttatattt attttaaacg tcgttgtatt 11700
cctactccgt ccccagacaa agcggatctg cgagtggaat ttccctcatt acccccgtgt 11760
gtcggcatac agtgcgctgc ataagaacac gcatgacaca tagcgtacct ctggacggta 11820
cagtatatga taacatgatt caaggaaagt atggattcct accgacatgt tatgacagaa 11880
cacacaggtt ttctgcgtgt tttataaaag agcgtctcga agcagcttga gccacactac 11940
ggtccagata acgagcgttg caaaaaatat gccgcgcagt agtcgaaagc cgtactgagc 12000
gtgcgaagcg ggtagggtgc cgaacgacgg atatgcgccg ttgtcatctt cgactataag 12060
gatcgcgacc gagtcttcgg gcatggtaaa agccacacgg tgtggttgat atgtagcgta 12120
tccggtttgg aatcgttcgg ctccggctca ggggatagtg aggaattctc aggggacgat 12180
atgggaccca atgactggat aaaagaaggg tttttcccag taagatgatc cccgtatcac 12240
atgaggtctg gatatatata aatgaggagt gaaataggca aagggtatca gacaccagcc 12300
tcgtcatgca gccgttggtt ctctcagcgg aggaactatc gtctctgcta atttgcaaat 12360
acatcccacc ttaagcgacg agtccataaa gcaccgttgt ccgggtacgg tgaaagtgac 12420
ccggattgta gcacgtccct tttttgtttt tgcatcgttt atcgtcacca ctagtgcaat 12480
attttgatcg taaggctgaa agagtattgt tatgatgctt agaacgtgga gattattaca 12540
gatggtactg cttgccacgt actgttatta tgtttttgcg aattgttcaa tcagcacgac 12600
gactgctcct gtggaatgga agtctcccaa ccgtcagatt cccaagaata ttacctgcgc 12660
taattactca gggaccgtcg gcggtaacgt tacttttcag ggtctcaaga ataaaacgga 12720
agacttttta tcttggctac tcgggtctgg ctataagtcc atttgctcgt tcttcccaca 12780
actccctggt gattctaatg agcagcatta cagatatgaa gtaaccaacc tcacgtacaa 12840
ttgtacctat gaccgcctaa cgttactgaa tctgacaatg gaaaacagca ggaattacta 12900
tttcagaaga gaagatgcga attccacctt ctactactct tgttacaatc tgaccgtgtc 12960
ctagagaacg cacgtgaagt tccacagagc cgcgtggctg tagctattgt ttacgttgct 13020
tttgaaatgt taagcgtccc tacggcgcta acatgtttct aggctactct gactgtgtag 13080
atcccggctt tgctgtatat cgtgtatcta gatcacgctt gaagctcgtg ttgtcttttg 13140
tgtggttggt cggtttgcgt ctccatgatt gtgccacgtt cgaatcctgc tgttacgaca 13200
tcaccgaggc ggagagtaac aaggctatat caagggacga agcagtattc acctccagcg 13260
tgagcacccg cacaccgtcc ctggcgatcg cgccgcctcc tgaccgatcg atgctgttat 13320
cacgggagga agaactcgtt ccgtggagtc gtctcatcat cactaagcag ttctacggag 13380
gcctgatttt ccacaccacc tgggttaccg gcttcgtttt gctaggactc ttgacgcttt 13440
tcgccagcct gtttcgtgtg ccgcaatcca tctgtcgttt ctgcatagac cgtctccggg 13500
acatcgcccg tcctttgaaa taccgctatc aacgtctcgt cgccaccgtg tagctagtta 13560
gccagctgtg tatagtttgt tgtgttttgc ttttgcgtat ttgttttcag tcagagagtc 13620
tgaaacgggg tgggagggac ttttgcgggt aatgcatgct aaaataaacg ggtgggctgg 13680
ggtgtgcttg gtaactcact gtttgaatac gcgctcacgc acatatgtag cactcaacat 13740
gttagctttt gcccgcacgc cccggggcgt gccgagctgc ctttttaata aagtctgggt 13800
ttccagatac gcgctggttc tgattttgat gatttgtgcc tctgaaagct ctacgagctg 13860
ggccgtgaca tccaatcgac tgcctaactg tagcacggta actacaacag cgggtcaaga 13920
cgctgaattg cacggtccgg caccgttaag ctgtaatgtg acccagtggg gacgttacga 13980
gaatgaaagc acacccgtat tatggtgcac tttatgggga tcacgcatgc gagtctcatt 14040
aggacaccgt gtagcgtttg gctgttcttg gaaaacattt tttatttata acgtttctga 14100
aagtagcggt ggcacttact atcaaaaagg ttacaactgc accgacaaac atataacact 14160
atcttgtttc aacctaacgg tggttcctcg agcggttcaa agcacaacca ccgtaatgac 14220
acccacggtg gttacaaact ccacattcag tgtgtcactt attgcgttga gactgacgac 14280
aaattccagc gcggttggac acgctagtta tcaacgacaa cagcgtgttg aaaacgggac 14340
gttatccaag aacataacta acttggcatt cacctatggc agctggggcg ttgcgatgct 14400
gctgtttgcc gccgtgatgg tgctcgttga tttgggtttg cctcaatcgg cttggcgacg 14460
ctggcaaagc cacgtggacg atgaagaacg tggtttgtta atataggaag taaaaggcac 14520
tgttttagca tgactgtttc caaaccgtaa cgtggtaaat aaatcatggc ttccgacgtg 14580
ggttctcatc ctctgacagt tacacgattc cgctgcagag tgcatcatgt gtacaataaa 14640
ctgttgattt tagctttgtt tgcccccgtg attctggaat ccgtcatcta cgtgtccggg 14700
ccacagggag ggaacgttac cctgatatcc aacttcactt caaacatcag cgtacggtgg 14760
tttcgctggg acggcaacga tagccatctc atttgctttt acaaacgtgg agagggtctt 14820
tctacgccct atgtgggttt aagcttaagt tgtgcggcta accagatcac catcttcaac 14880
ctcacgttaa acgactccgg tcgttacgga gcagaaggtt ttacgagaag cggcgaaaat 14940
gaaacgtttc tgtggtataa tttgaccgtg aaacccaaac ctttggaaac tactccagct 15000
agtaacgtaa caaccatcgt cacgacgaca tcgacggtga ccggcgcgaa aagtaacgtt 15060
acggggaacg ccagtttagc accacaacta cgtgccgtcg ctggattctc caatcagacg 15120
cctttggaaa acaacacgca catggccttg gtaggtgttg tcgtgtttct agccctaata 15180
gttgtttgta ttatggggtg gtggaagttg ttgtgtagta aaccagagtt atagtaatgt 15240
gttttttatc agggagaagg ttttgtacca acaatgacta catcggggct atctgtgtcg 15300
gaaaattatg acggaaatta tggactcacg gaaaccgcca atacaacgcg tacaaatagc 15360
agtgactggg taacgttagg aaccagtacg ccactgttgg gaagcacgga gactgcgatc 15420
aatttcggca acgcaactac ggttattcca caacctgtgg aacacccggc tggagaagta 15480
cagtaccaaa gaacgacaac gcattactct tggatgctga ttatcgttat cattttcatc 15540
atttttatta tcatctgtct acgagcacct cgaaaagttt atgatcgttg gaaagacagc 15600
agagagtacg gacaagtgtt tatgacggat acagaactgt aatatactat gatgtctaag 15660
aagtgtttgc ggttatttcc atggatgaca attttgtttt gcataccaaa agcacaacat 15720
tggaactata tgacaatacc atgcgttctt aaaattggac gcggcggtca aaatatgagt 15780
ttgcctcccc ttaacaattc attgtacgga aacgatattt ttcaatggta tacagacaga 15840
ccgacagtca ccaacacgtt atgtctttat caaaacaatg agtactacac acaatccaat 15900
gaagatattt caaacatcaa atggcaatgt acaaaaaacc atacgttaat tcttattaac 15960
ctaaccgcaa catatagtag gaactattac tttcaatctc ttaaaactct tgggcaagga 16020
ataccgagac cgagcagctt atgttataat gttagtgtac accttaccca ccaaacacat 16080
tgtcatacaa ccacattatc cctgtatcca cctacacctg tacacaattc attaacaata 16140
tcaccgtcat tagcttcaac caactttaca catgttgcgg tccatcatgc cgcaggtaac 16200
gttgaagcac aacacaacac tgccactcca catacaacgt ggatcatacc cctggttatc 16260
attataacaa tcatcatttt aatttgtttc aaatttcccc agaaagcttg gaataaattc 16320
acacaatacc gatacaacag tatgctcgcc gccgcttaaa gaatcaccgt cgaggaaact 16380
aaaagctatg tacgtttatt tttcagctca ctgtttgaat accgtaaaca taatgacgta 16440
catatacgtg gttatacaac aggtgtttgt gttatgcggc gactgattaa ccatatcgtg 16500
aaccatgatc ttttccgatg gtctgtcgtg accgcaatga tattttacaa gtattccgaa 16560
acctgtatgg aggtcactgt cagagtaggt gatccagtta ccctcggtag tggacatggt 16620
tatcatccag gacaaaaagt acactggtat aaccagtcat gcgtcggcat cagcaacggc 16680
gaaaatacgc atcctatctg cacctacgac cctcctaaac ctggtagaca aaagacaatg 16740
aaaaccactc cgttgccatc accactgttg tatgaatgtc acaattccac attaagcatt 16800
cttcatgtaa acgtctcaga tcccagaaac tattgcaggc gaaaatgtcc accaaagggt 16860
aactgtgagt ttcccacatg ttttacatta tcgctgattt ctagaacgac aaccaccaga 16920
agacccggac aaaaaactac gctgtcgcga ttaaaaacta cgccaaataa acatacgcag 16980
cacaaaagat ccacgcgaag aacgtcactt aaagattaca atgtaacggg tctgccgaaa 17040
ggctttgcgg actcgtttac cggtaacgta gaggcacata gagccaaaga tgccgcacac 17100
agcgcatgga ttctcattgt catcatcatt atcatagtcg tcattctgtt tttcttcaag 17160
attcctcaaa gactccgaga gaaatgggac accaagggat acctttacaa agggaccgat 17220
ggcctgccca ctacggacta attatcgtga gcggacggat atgtccggtt tcaaactcac 17280
tgtttgaata tagggacagt ccctacggaa cctgagaaca tgtggaaatc acctgtggta 17340
gaatgctgct caggtacatt acctttcatc gcgaaaaggt actttaccta acggctgcat 17400
gcatctttgg tgtctacatc agcctccatg atgcctgcat accggtggtt ggcaagatag 17460
gtaccaacgt tacgttgaac gcggtagatg ttctttcccc tcgcgatcaa gttcgttggt 17520
catacggtcc aggcgggcaa ggctacatgt tatgcatttt cactggcaca tcaacaacaa 17580
cgtttaacag cacgcgcttt aatttttcat gtctgagtaa ttacagcctc ctcctcatta 17640
acgttaccgc gcagtatagt actacctatc gtactatgac atcgctagac gattggcgtc 17700
accaaaaaca taaccatggt tttcgatgga ctttagacac atgttacaat ctgacagtga 17760
acgaaaacgg tacattcccc actaccacca ccaaaaagcc cactacgact acgagaacga 17820
caactaccac aacacaaaaa acaaccacca cgagaacaac caccaccgcc aagaagacga 17880
cgataagcac tacccatcat aaacactcca gtcccaaaaa atccaccacc cctaacagtc 17940
acgtagaaca tcacgttggt tttgaagcca cagcagcgga aacaccgtta caaccaagcc 18000
cacagcacca acacgtggct acacacgccc tctgggtttt agcggtcgta atcgttatta 18060
tcatcattat cattttctac tttcgaatac cgcaaaagct gtggctgctc tggcagcatg 18120
acaagcacgg catcgtgctc atcccccaaa ccgatctgtg agcaagtcgc gtaggaaacg 18180
attgcatgaa atcactgtga aacgccaact ccgtgccaac tggcacggcg gacaggcctt 18240
tgacgtattt gaagccaggc gcgctcttga taccgaaagg atccgagggg gctttccaaa 18300
gccgacgtcc ctgattccct tcataaagct gttgaccggc cctagaaaga ccaagagcat 18360
gctgtgggcc cactgcggtc gcttcttgcg ttatcatctg ctcccgctgc tgctgtgtag 18420
actgccattc ttactccttt ttcagcggcc gcagtgggcc cacggcttgg acattgtcga 18480
ggaggacgag tggctacggg agatacaagg agcgacgtac cagctgtcca tagtgcgcca 18540
agctatgcag cacgccggat tccaagtcag agcggcgtcg gtcatgacac ggcgaaacgc 18600
cgttgacctg gaccgaccgc cgctttggtc gggatcgctc ccgcatttgc ccgtctacga 18660
tgtgcgttcc ccgcggccgt tgagaccgcc gtcatcacag catcacgccg tatcacccga 18720
actgccgtcg cgaaacggga tacgttggca gtaccaagag ttgcagtata tggtggaaga 18780
acaacggcgg cgaaatcagt cgcgtaatgc gattccgaga ccctcgttcc cccccccgga 18840
tccaccatcg cagccggcag aggatgcacg agacgcggac gcagaacgtg ccgaatcacc 18900
acatagtgca gaaagcaccg tcaggcacga cgcgagtgag aacgcagtgc ggcaacggcg 18960
cgaaagacgg cgctataacg ctctgacggt ccgcagccgg gactcgctgc tcctgacgcg 19020
aatacgcttc tccaaccaac ggtgtttcgg acgcgggcgt ttgagacatc ccgcgggaag 19080
tggtcccaac accggcggac cgcgacccgg cggtgcggga ctccgtcaac tacgccaaca 19140
actgacggtc cgctggcagc tgttccgcct acggtgccac ggttggacac agcaagtctc 19200
tagccagatc agaacccgct gggaggaaag caacgtcgtg agccagacgg ccacgcgagt 19260
acgtacgtgg tttgtggaaa gaaccacgtt ttggcgtcgc acgtggattc cgggacagaa 19320
cccggcggcc gaagcgcaag aactggccgt cataccgctg gcacccacgg tgctccagca 19380
gaacgaggaa ccacgtcaac agcttacggg agaggagaca agaaattcaa cgcacactca 19440
acgtgaagaa gtggaggacg tttcgagaga ggacgcgaga gaagggaatg atgggagccg 19500
agcaagtgga aacgacgaga gaaggaataa tgcgggaaga tatgatgatc acgaggttca 19560
agagccgcag gtcacttatc cagcgggaca aggagaactg aacaggaggt cacaggagga 19620
gaacgaggaa ggtggaccgt gtgaatcgcc gccaatgacg acaaatacgc tgaccgtggc 19680
ctgtccgccc cgcgaacccc cgcatcgtgc cctgtttcgt ctatgcttag gactgtgggt 19740
ctcgagctac ctggttcgac ggcccatgac gatttagaat acaccgagcc attcctttat 19800
ttcccccccc cccatccccg gtcgcttatg cgtgtcaaac actaccaata aagataatct 19860
gccaatagca ccttatatat aatatgtggt cgcgtgtggt ctttttaagg agccctgaaa 19920
cacagacagg tatgggcggt ggccggctgc cgccgctgtg gctgccgcta ctgatcgcct 19980
ggagcgagtg gggcaactgc tgcctcgatg cgcctccggt ggtgcgttcg ccctgtctgc 20040
agccggtgcg cgaccgcaac cgcgagcgga acccgggctc accgcagttg ctgccttacg 20100
gcgaccgtct ggaggtggcc tgcatcttcc ccgcgcacga ctggccagag gtctctatcc 20160
gagtccacct ctgctactgg cccgagatcg tgcgttcgct ggtggtggac gcacgcagcg 20220
gtcaggtgtt acacaacgac gccagctgtt acatcgccgg cgggcgctgg cgttttgagg 20280
acggcggcgc ggcgcagcgg ctgagcctct cgtttcgtct catcaccgag accgcgggca 20340
cctacacctg cgtgctgggc aacgagaccc acagcctggc gaccgagacc acggcgctgg 20400
tggccgacgt gcacgacctg cgccactcgg accgctcctg cgacctggct ttcggatcgc 20460
gctcacagac gcggtacctg tggacgcccg atccctccag gttgcgcagt ataaactgcg 20520
gttgggaggg tgaacggcac cgcgtagtcc actacatccc cggcacctcg ggtctgctgc 20580
cctcgtgcga ggaggacgag cgcgaactgt gcgtgccctt catcagccat agcatcgccg 20640
acaacaactg cagccgccgg catcgagtag acggcgctag gcggcgctat catctgcgga 20700
gggattactg gctgacggat ccgaagatcg ggctgctggc cgcgggatcg gtggccctga 20760
cctccctctg ccacctgctg tgctactggt gttccgaatc gtaccggcgc ctgaacaccg 20820
aagaggaaaa cgaggcggcg gaggaaactg ccgcgggaga agcctctgcg gtagcggcgg 20880
cggccgtctc tgaggaagag cagcagcggg agtaaacggg gagagccatg aagcggatga 20940
ttcgcagtca cggcaggaaa acggaatgtc agatgacggg cgccggcgag cgacgcggct 21000
ccgccgtcgg tgcgctcatc tgcgacagcg gtacccgacg cggcagcggc gccaacgaac 21060
gccgcgactc cgacgtcggt cccatcgccc acagtagcgg taccagacgc ggttcggcaa 21120
atgaaacgtc cgcctgtacg cggaccgatc accagaaggc ggacattggg ctgtggttca 21180
tgtttctgtt ttttggactg tgttcgtggt tagcgatgcg gtatcgcgca caataaattt 21240
tgaatccata tcaaggaacg cgtgttttgt attttattgg gaatattggc ggggataaac 21300
cggtttcgga tgtttaccct taatcttacc ggggacctcg ttgtcctctc ccccttcttc 21360
ctcggacacc gggcttcatg ctgacgtagg taccgactgg ggtcaaaagc ctgggtactt 21420
atggggagcg cgcacaaagg accgtcaggc gccggcatgg agcgtcgccg aggtacggta 21480
ccgctgggat gggtgttttt tattctttgc ttatctgcct cttccccgtg tgctgttgac 21540
ctgggtagca agtcctcaaa ctctacctgc cgcttgaatg tgacggagtt ggcctcgatc 21600
cgtcctgggg aaacgtggac gttacacgga atgtgtatct ctatctgcta ctacgagaat 21660
gtgaccgagg acgagatcat cggcgtggct tttacttggc agcataacga gtctgtggtt 21720
gacctgtggt tgtaccagaa cgatacggtg atccgcaatt tcagcgacat caccaccaac 21780
atcttgcaag acggactgaa aatgcgaacc gtccctgtga ctaaactgta caccagccgc 21840
atggtcacta atcttaccgt gggccgttat gactgtttac gctgcgagaa cggtacgatg 21900
aaaataatcg agcgcctcca cgtccgattg ggctcgctat atccgagacc gcccggatcc 21960
gggctcgcca aacacccctc cgtaagagcc gacgaggaac tgtccgcgac cttggcgaga 22020
gacatcgtgt tggtctcggc catcactctg ttcttcttct tgctggccct acggatcccc 22080
cagcgactgt gtcagcggct gcgcattcgc ctgccgcatc gataccagcg gttacgcacc 22140
gaggactgaa cggataaccg caaaggccac gtgcaacgtt cacgctgcta taagaaggcc 22200
atgtcccccg tggacgggtc tctttgacac gagcgcggca cgccgttgcc acgagcatgg 22260
atcacgcgct cctcacacac ttcgtcggcc ggccccgtca ctgtcggttg gaaatgttga 22320
ttctggacga acaggtgtct aagagatcct gggacaccac ggtttaccac aggcgccgca 22380
aacatctacc tcgacgtcgc gctccgtgcg gcccccagag gcccgccgag attcccaaaa 22440
gaagaaaaaa ggcggccgtc cttctgtttt ggcacgattt gtgctggctg tttcgacgac 22500
ttttctttcc tcgggaggac tcggagccac tgatgtcgga tccggcacgg tctcccgaag 22560
aggaggagta aacaacacac ggctaagagg atacatcatc aaagaagata ggaggggtca 22620
aaacgcggac tgaaagtata taacgccgat catgtccgag gaactgttaa taaaacgcca 22680
tgatgacaat gtggtgtctg acgttgtttg tgctgtggat gttgagagtg gtgggaatgc 22740
acgtgttgcg ttacgggtac acggggattt tcgatgatac atcgcatatg acgttgaccg 22800
ttgtggggat ttttgacggg caacactttt ttacctatca cgttaattcc agcgataaag 22860
cgtcaagtcg ggccaacggt accatttctt ggatggctaa cgtctcggcg gcctacccca 22920
cctacctgga cggggaaaga gccaaaggtg accttatttt taaccaaacc gagcaaaacc 22980
tgttagagct ggaaattgcg ttgggttacc ggtcacagag cgtgctgacg tggacgcacg 23040
agtgtaatac cacggaaaac ggtagttttg tagccggtta cgagggattt gggtgggacg 23100
gggaaacttt aatggagctc aaggataacc tgacactatg gacgggcccc aattacgaaa 23160
ttagttggtt gaagcaaaac aaaacgtaca tcgacggtaa aattaaaaac atcagcgagg 23220
gggatactac aatacaaagg aactatctca agggtaattg cactcaatgg tccgtcattt 23280
atagcgggtt tcaaaccccc gtcacccacc cagtggtaaa gggcggtgtc cgaaaccaga 23340
atgacaacag agctgaagca ttctgtacat cttacgggtt ctttccaggg gaaattaata 23400
ttacttttat ccattacggt aataaggcgc ccgatgatag cgagcctcaa tgcaatccgc 23460
tacttcccac cttcgatggg actttccatc agggatgtta cgtagccatc ttttgcaatc 23520
aaaactacac ctgccgcgtt acacacggta attggacggt ggaaatcccc atcagcgtta 23580
cctcacctga cgacagttcc tcgggggagg tccctgatca cccgacagct aacaaacgct 23640
ataacaccat gaccatcagc agtgtcctcc tagccctgct tttatgcgct ttgctattcg 23700
cgttcctgca ctactttacc accttgaaac aatacctacg taacctggcc tttgcgtggc 23760
gctatcgcaa ggtccggtcg tcatgaccag caacgccctg tatgagctgt ttcgacgtcg 23820
gttaccgcgt gcccccgtca acacggtcat gtttctcacg cgacgcactc gtgatgggtt 23880
ctgcggtcgg ttgacgtcca tcgccacgaa ttcccactac actatgttcg tgttagatca 23940
cggatccgtg cgcatcgagc gaccgagtca gtcagaagtg gattgcgcca gtttaatgga 24000
aacgctgaag cggattcggt tacgaaattc gtgggtagcg tcagaagacg agctagatgt 24060
gagtcgcagg gacgcgtgac acgaaacgcg ttcaggatta acgtaggttt tcgaaataac 24120
ctacgtccgt gagtgacgcg gtttcgtgtt gaaacccgcg cccgcttctc acggtggttt 24180
atgatgaaac cggcgttgcg gatccacgcg ggttcctcat tcaacctgcg aaaagaggaa 24240
gttgcggtaa aaccacgtca ataaagacgt caatgacacc tcaatgttgc gttggaacgg 24300
tctttatata tacaaacgcc gttatgatca gtgtccggca agatgctcgg gatacgggct 24360
atgctggtga tgctggatta ctactggata cagttgataa cgaacaatgg cactcgaagc 24420
aacaataccg ataccatctt tgtatctctc cttaccgggc ccaacggagt tactcgcaca 24480
gccatcggag gtctgtattc aaactacacc aacttaactg gagcatttgg cttcacttca 24540
acaaatatgt cagcaaccaa ctcttccgct gaggataatt ggagcgtaac caacctgacg 24600
gagagttgca tcaaccgcgg tgagtcctat gtgactacca tctggcttct ggactgcact 24660
aaaaacgata cttattggta ctatggaaat gcctacaatc atacatgtga aggtacaatt 24720
tcgggatatc tcctgggcat gtgcaagcta tggaaaagtt gggtcaataa tattacttct 24780
tataacactg tcagagtcga atcgctggga aatgaaacac ggtgcatgct gctccctaga 24840
cagtatactc tcaacgccac ggtggaatgg tacaacaaat ctgaaggtga cgtaccagaa 24900
gaattcatgg actatgttat cctgaccccc ttggctgtgc ttacatgcgg actgcaggaa 24960
gcttatatac tcgacaaagg tcgtagatac atgtatttgt tttccgtgtc ctgcgtggga 25020
atcacaggta ccgtatctat tatactcgtc tccctatcgc tgctcatcct catctgttac 25080
tatcgctgtg gccggcttct gatatgccca cgcggctttg aactcttgcc agaattcact 25140
gaggaagagg aggaaaaaga aaaattgtta acgcataatg acattgaagt ccaagtgcct 25200
attcgcacgc ggcgactact cgtcccttgg atccgggaga gcaaaatgtg ggtactacca 25260
cccccgttgc ctccacgacc tccccactta atagaattcc cgccgtctcc tccgtcatcg 25320
cctgggccca tgcacatggt ggtctgcatg ccagcatgac gaactttgga ctctgagccc 25380
caagcggtac gaactacata ttttccataa atctacactg aacttgagca caaagatact 25440
gacaatagac tggatataca gacttttata tgatccctgt acagatgtaa ataaaatgct 25500
tttatttaaa actggtccca atgttcttcg ggaatcatgg ggtggggacg ggggacgcgg 25560
tagggagcaa aaccgggtac atggggggga acatcgtcca acagtagcac cagcggattg 25620
ggtaggggtt gctgcggagg tcggtcgatg acgatgtcga tctccatcgg cagatccggc 25680
aacatctctt catctccctc accgaccagc actcggcgct gttctggatg tatatgattc 25740
tggaaaagcc tccgacgagc tcgcggcgcg tagaaagcca agcggcgcaa gggccggcga 25800
gcccgaaagt ccatgcgcac agatggcatg agtccttgag tgacggtggt gagctgggga 25860
acagggctac ctcccatcgc gacggtgaca gtggatccat gagagaggcg ccgcacgctg 25920
catggctaaa taccgtgaat cccctgacgt cgtctttcgt cccgaacgcg tcatgttggg 25980
ggcgaggcgt aaaccgtcga ggttgaaaaa ccgcgtatct gcgacccgtc cggactacgt 26040
tgttttttag aagcggccac atgacctcga gatgtcgtca cccaaggtat ttaacggcac 26100
acagccagac gcgttcgtca gcagcgacgc cgacaagacc tcagcatggc tcggaggcta 26160
tggatcttga gcttactagc cgtgaccttg acggtggctt tggcggcacc ttctcagaaa 26220
tcgaagcgca ggtaaacgga atctggggaa ttcaacacag gtaagaaata taaaaaaata 26280
acgtgattgt gaacgcggtt atcgtgtttt tgcagcgtga cggtggaaca acccagtacc 26340
agcactaact ccgatggtaa taccaccccc agcaagaacg taactctcag tcaggggggg 26400
tccaccaccg acggagatga agattactcc gggggagact atgacgtttt gattacggat 26460
acagatggag gtaaccatca gcaaccacaa gagaagaccg acgaacacaa gggagaacac 26520
accaaagaaa atgaaaagac ccagtagcag cagcagatcc caagggttaa agaccatgtt 26580
gactattttg ttttttatta aaaagctgta aggttttgct ctaaaaacac cccgcctccg 26640
gtcttttttc ttttgtattc ggcacgcgaa acacggtttc ttcccatagc ctgtctaact 26700
agccttcccg tgagagttta tgaacatgta tctcaccaga atgctagttt gtagaggcta 26760
tgcgggatgc tgcggcggcg cgaccttccc tctccaccca gccccgtcaa aacacacgcg 26820
actcgagcgg ttcgtatgaa aaataaaaaa cagcttttta tttacaggaa cggggaaaaa 26880
aaaggcacac ggtccgtggg agacgcgggt tcacgcgtcg tcaaaaagtt ggtggtccac 26940
tccgtaagga caggtaggct tatttagctt ccgcatgctc ctggttccgt aataaatgcc 27000
gttttcgtgg cagcgtgtca tgccgcgagt cacaaactcc atcaaactgt cggccacgat 27060
gcaaacgtgc tgattgttgg cagcaaagac gcgcatacag tcgtccacga agaggttgat 27120
cacgtcgtag gggctcacca accagcctaa aggttccacg tggttactgc cgaccatgac 27180
cctccagtcg ttaatctcgc tccagtcgta cagccgaatc gtggagacgc gaatgacgct 27240
gtaatcaccc atgaccatga gtcggccgcg atacgtagca cgccactgcg cgaacgcgtg 27300
gatgtgcatg cagccggcca gcgctctaag cgaggcggtg tgcggcagct cctctgggac 27360
ggtgatgaag ttgcagcgtc gcaaaccgat gttgagaaat tcagtgatgc tctcggccac 27420
aaaggtcaac gagtcagagt agatgtggtc ggtccacagg tacatggcgc ccgaggcgcc 27480
caggtacagt tcagacggca cgttgtgatc gcccttgtgt ttaagaaagt tgtaggtgca 27540
gatgctgccg acgaaacgca gcggctcggg gcagcagagg tagctggcca gacgctgtgc 27600
atcccgtcct tcgtcgcgca ccaagcgcca gcgacgccgg ataacgaggc agcggtcttt 27660
gggccagacc agggccacgc gttgcccggg tttccacggt cgcgacgtct taggaggcct 27720
ccagcggtcg agcagattga gaaaacagtc cttgattacc gacatcgcgg tcgcgcgtcg 27780
gtggacaaaa agaaatcggg ccgatccaga aaaaaaaaaa acgacagcga aacaccgccg 27840
tgctcgagcg aagggtggcg gagggccaga agaggcggcc ttgacgacgt tggcagcgaa 27900
aaaattggca cgcgagtcaa acgggaagta gcgtcggtgt tttatgcccc aagcagcgtc 27960
gtcgtcactc gtggcgtcac agtcaacggt gctgacgtcc tttggggcag tcgggcacgc 28020
gatcgtagat gccgttgtgg ccgctgaaac gtcggttttc aaacagcagg ttaagtccca 28080
gacacatgaa cgtgttcaga ttatctccca cccggatgta gcggtcgtcg cgcacgtcgc 28140
aggcgtagac ggccccggta taggcgacga cgatggggat aaggtcgacg ggccagcgca 28200
ggtgaggaaa gggcgcgttc tcgcccttga ggctgacggt tcccaggccg agaacgcgca 28260
ttccgaaagc ggttttgatg ttgcgcagca agtgaccgcc ttccacgctg ttttcgaaac 28320
acctgaggtt gcatagacgc agttccgttc ccggcgggaa cgtcaatggc atgaactgcc 28380
cgtggtggcg gatgatgaat cgtgccatgg tatccaaacc gaggctccag gcgcgcaaca 28440
gcgggcgaaa gtagcgctta accaacgacg aggtcaggta gcgcatgcag tgcagggttt 28500
cgacggcgcg cagcccgacg cgcgcaaact ccatgaggtt gcgggccagg tagtagacgg 28560
cggtgtcctc gcgtacatag caaaagacat agccctcgtc cgagatgagg cacacggcgg 28620
tcttcttctg ctgatccggc gacaacacgg cctcgttcac gaagcgaccc acgaaggcca 28680
ggcgcgtctc gcagcacagg tagtgactcc aagctttcac gtcctccggt ttgaagtcct 28740
cgtccgtctc gatctcctgc agcactaggt tccagcccgg cggccagacc acgggcaaca 28800
cctggcctgc gttgatgcgc acgtaagctt ccagacagcc caggccgaac tcggccgtga 28860
gcgccaggct agccagatcg ctcatgtgac gcgccgagtc ggtgggcgag cccgggggcc 28920
cgtcgcacac cacgctccgt cttcttgtcc tcaccgcggc cagcgtggcg aggacacttt 28980
ccgcgcccga ggctgtatct tcggtttgcc cgccggagcc ggccctcact atataacgtc 29040
ccgcccgggt ctcctccatg tatgcaggta agcaactgag ccgaacgcac ctcagcagac 29100
gagaggatgt cgtcgcggcg tcgcagctcg tcacgtcgct ctggcgaacc ctcgacggtg 29160
atttatatcc cctcgagcaa cgaggacacg ccggcggatg aggaggcgga ggacagcgtt 29220
ttcacgagca cgcgggcgcg cagcgccacg gaagatctgg atcgcatgga ggccggtttg 29280
tcgccctaca gcgtctcctc ggacgctccg tcgtccttcg agctcgtgcg cgagaccggc 29340
ggcaccggcg ccgccaagaa accgagcgaa aagaaacgat cgtcgtcacg tcggcaaccg 29400
cagatcgcag cgggcgcgcc tcggggctcg ccggcgacac ccaaggccgg caagtcgcct 29460
aaagtctcgc gaccgcctag tgtgccctcg ctgcccgaga acggcgccgg cggcggtggc 29520
gacgataaca gcagcagcgg cggtagcagc agtcgcacca ccagtaacag tagcagaagc 29580
accagtcccg tggcgccagg tgagccgtcc gctgccgagg gcgatgagtt ttccttctgt 29640
gacagcgaca tcgaagactt tgagcgcgaa tgttaccggg tcagcgtggc cgacaatctg 29700
ggcttcgagc ccagcgtggt cgcgccgcag cacgtcgagt atctcaaatt cgtgctgcaa 29760
gactttgacg tgcagcacct ccgccgcctc aacgaatgca tacccatgcc ggccttcgcg 29820
ctcaccagcc tcgtcgaccc cgtcttaaac aacgtagcgc ctggcgagcg cgatctcacg 29880
cgtcggataa tcacgcacgc ggtgatcatc aactattact acgtggcgca aaagaaagcg 29940
cgccacatgg tggaggccat acggaccacc gtgcgggacg acacggtacg ccgggtagcc 30000
gcgcaggtca acaaccagag ccgttcgggg cgtgcggccg cgctagcgct tcactttctc 30060
acgtcacgaa aaggagtgac ggacggtcag tacgccacgt ctctgcggcg gctggacgaa 30120
gagctgcggc atcgcggcac gcccgaatcg ccgcggctca ccgaggtcta ccagacgcta 30180
cgcgattaca acgtgctctt ctataccgcc cactacacct cgcgcggcgc gctctacctc 30240
tatcggcaaa acctgcagcg gctcaacgaa aaccaccggg gcatgctccg gctgctttcg 30300
gtcgaagaga tatgcgaaga gcacacgctc aacgatctgg cgttcctagt aggcgtcgag 30360
cttatgatca cgcactttca acgcaccatt cgcgtgctgc gctgctatct ccagcaccag 30420
ctgcagagca tctcggagct gtgttacctc atctatgtac aactgccgtc gctgcgcgaa 30480
gactacgcgc agcttagtga cgtgctctac tgggccgtca gtcaaaacta cgactacgcg 30540
ctctacgcga gcacgccggc gttgtttgac tttttacgcg tcgtgcgtca gcaggacgcc 30600
ttcatttgca ccgactacgt gtactgcgcc ctgcgtctgc tggcctgtcc cgacagacct 30660
attatcggtg acaccggcgg cagcagtagc tcccaacgcc tcgtaggcga gtttatggtg 30720
cgcgatccgc tgttgcgcga cccgcgcgcc acccacctgc gccagaaact catcacccgc 30780
gacatatgcg tggcgcggtt gcaagcgcag ccctcgagtc gacacattcc ggtcgaacac 30840
acgggtgtct cctccgtcac cctgctcaag atctttagcc aggtcccccc cgacgaacgc 30900
gaagaagaca cgttacgcga gatggctctt aaagcgttta tggaagcgaa cggtaatcac 30960
cccgaacaaa tctgccgatc cccaccaccc ccgctgccac cgcgcgacta tcctcaacgc 31020
gacgagcggg accgtcaccg tcgcgaccgc cgcgacagcg gggaatactg ttgctgatgg 31080
tgggacgaaa cagcagggcg gaacagttta tgatagaaag tcacaggaaa gtatgtgttg 31140
tttttttttt aatgtaccaa gaataaaaag tgcgtctacg accaaagcgg tgtgtggacg 31200
ctcgtcctct gtcttctccg gttttttttt atgtgtgtgt ttttcttttc cttcctattt 31260
tgttacggca acagcgctga tggcacgttg ccggcttcga acatcgcgtc ggtgatttct 31320
tgcttgcccg gcgtcacacg gtgacgcagc agcgcgcggc tcacgtagca ggccgactcg 31380
cggatgacct ggccgtcggc gtcgcgtcgc aggcccgagc ggttgccgtg acgcagtcgg 31440
ccctgcgcgg cgcgctccac gtcttcaaag tagctgtgta gcaggccgcg ctccagcagc 31500
tgcggcagcg agtcggcggc gcgcaccaca aagttctcac ggctgatctc gtagcacagc 31560
acgctgccgt cggctgccac gccggccacg ctgcggtccc aactgaagag gttggcgagt 31620
ccgatggtgc cgatgacgcg caactgaccc tgggtcacca ccagcagctt ccagtattct 31680
acgtcgcgcg gggtgaggat ggtctcctcc acgtcgcaga caaacaacgt gtagccgcgc 31740
ggatagggca gatccaggtg gcgaccgcgc tggcggcgca taaaatcgtc taaattcaaa 31800
ccgccgtcgg gtgcgcgcct gctcgtcatc gccgcgcctc gtcggtcgat gaccccacgg 31860
tgcttataac gcgccgccgc ggcttcatgt ggcgtgacct ccgacctcgt gaggccgaaa 31920
acggcgtaca tgaagacgct caaacttttg aatgtgggcc cggtagcgca ccgagggccc 31980
cggggcggcg acgacggcgg gtccgagttc cagcggggcc ttgcggcggc agcggttggc 32040
gtggttgctc agctcggcgt ccgagagcgc cgagctgaac tgcggcagcc gcgtgcgatc 32100
ctgcggcgcg tccccgtgtc gcagcgagtg ccagagcagg cgctggacgc gcgccgtctc 32160
gggcgtcggc ggcgcgcgac agccccggcg cagcttgaaa acgtgcaggc acagcagctc 32220
gcgcttgatg cgcagcgaca cgctgcggta gtcgggaatc cgctgcacca gctcgagaaa 32280
gtcgcagaag gtctccacga acgtgtcctc ggtgaagcga atgcgcttca gatcgtggac 32340
gtgtttgcga aaccgcgaca gttctcgacg ttgcacgggg ttctgagcga gtcccttgcg 32400
cagcagcgca gcctcgcctt taaacagcct gatgagccgc tgcacgtccc cgctcaacat 32460
acgtatacac gccgtgtact cgtgacgtat actggcgcgc agcagccgaa tgatacgcag 32520
ggccagcacg gcgttggagg ccaggtacat ggcgtagccg cgacgcgggt tggcacaggc 32580
ccagcccgcg gggagcagaa agtagtcgtc gaccagcgtc tgcgaccagt cggcgaagcc 32640
caggtcacgt gatacgctgt cctggacgcg ggccacgtcg ccggctgtga ggtggcggat 32700
cgccggcagg tgaaacgcgc ccaggtgtcg attgcgctcc agcctcagct cggcgtgctc 32760
caaacgggaa tggtgggacg ccaccgcgga gggcgacaaa gaggagtggt cgccgccgcc 32820
gtagttaccg ttgtgattac cgccgtcgtc gcgcccgtcg ccgcactcgc aaaaggccgc 32880
gtagaggtcc ttcaacgccg cttcggctcg cgccataaac gtggcgtgga aaaaaacggc 32940
ggcgcggtgc gtccggtact tgacgggcaa cccgcggcac agggccgccg gcaggcagcg 33000
gccgatgagt tcgcgctcct cgggctccag aaacaggcac agggtgccgt ccaggcgcag 33060
gtacagctcc tcggtcatcg agcatagctg ccgcaagtaa tgggtgcgcg tcccaaaggt 33120
cttgtaatcg agcaacgtgc acaccacgta ttgccccgtg gccacggcca gagcgatgcg 33180
tttggcggcg cgactgatct ctggcaagta ctgcgcctcg tgcaccagac ggcggaaagc 33240
gccggcgttg agccagcgaa aatgctgcgg atcgggcggc aagggcacgc ctcgaagcgc 33300
ggcccagaca gcgaggtccg actcgagcgt cagaccgcgg atgtcgtact tgccgtgcgc 33360
cgtagcgcag gctgaatgga ccagacagct gcggcgaatg tacaccatgg cgtgcttggg 33420
atgtttgggc gccggcgttt tctttttctg accgccggcg gccgccagat cctcgggcgt 33480
gcgacacaac aggccggcgc gcacagcctc ctgtcgatta cgaatcggcg tcaggtaggc 33540
gcgcaggaac tggtgacaaa actcctcatc atcacgacag tcgtcgagat actcgtacgt 33600
ggtgagcgga tcgcgaaata ggcgctcgtc accgtcgtca tggtcttctt tagcctgctc 33660
ctccggctgc tgggttggcg gtggaggcgg cggctgatcc acggggttca tgactgagag 33720
gaagaagaag gtggcggcga agcgacgcgg agcgacggcg gtaaagccag acaccggcta 33780
tatagctagt catcacagtc tcctccttca cgacgccccc gtgccgctca cgctatccag 33840
cacgctacgg cccgaaaaca cgtactcgct gacgtcgtac gcgggcgatg tatggctgct 33900
caccggtttc gcggcgacgg ttgcgctcga gtccaacggc gagaagcaaa aacgccgtgg 33960
gcaacgaaac cagaaggagc cctgacggat aaaaccgcgc agcgtctcgg ccaacttaac 34020
cagcatcgta ccgtacagca gtacgtgaat gccgccgtgc gcgtccataa atacggcttt 34080
gtttacgggt tccatccatc cgatgactac aaagtgagcc tgttctagca cgccgatcac 34140
aaaattgttg gcctcgtcgg cctcggccac attccacgag ccgaaagtga aagtacaagc 34200
gggcgagccg cccaggcgga ttttgctacc ggcgtggagc tgacatacgc gcagcagatt 34260
ggcgcggtcg tgcagtatct gggagagttc gtacatgccc gcgaaggtgt gcttaaacca 34320
cgcgccctct acgatttcat ccacgtaatc gcgctcaaag aagctataca cggcaaagag 34380
gccgttctca aaaaactcgc cgaacgagag ccccagcacg tacaccttgt cctcgccggg 34440
caggtacgca aaggcgtgcc cgtgcccgga gacccagatc tcgggcgccg tgtttgcgtc 34500
cggcacgcat tcgtacacac tgacgaggcc gataaagtac aagcggccag cctggcgcag 34560
gcacgagaag cgccggtagg tcttgtgatc gcgcaccacc ccaaagtact gagtgtcgcc 34620
cagcatgatg ccgtgcagcg gcggccagca cagcgggagc caacgacccg ccgtggcgcg 34680
cacgtagcgc tgcaggtgaa ccccgctcgc acgctcgcgc ggcttcgggc gcttgtgggt 34740
ccaggcatca cgcagaccgc gccagatgct gctgaacttg ggctgcccgc gcagatagag 34800
cgacgagagc gagtcaaagt agcccacgac gagcctgtcg ggagacacaa gagcgcgaaa 34860
atcaaaccta gagcgacgac ggtgaaaaaa ccgattataa gcgcgtgtct caaacacgct 34920
actttcggtt ataaaaacac cgtcgcccta tttctgggcg tgtgtacact gatgactcac 34980
ctacgctttt tgaacggcag tctcagctcg ggattggcct cgtacagcga gctgcggtcc 35040
acggggccga tgctctcgta gcgaaagtcg tcgatgagca gcgccagccc cacgcgcacg 35100
aagcccctga ggtcgcgcgc cagccgcacc aacttatcct gccccaccag cgccgcgtac 35160
acggtacccg tgtcgccgca gagaatccgc acgcggtgaa agaaggtctt gtcctcggcg 35220
ccctcgatct cgcccagcgg catgacgggc tcgcgcgtgt acaacgaacg ttgaaagcgg 35280
cgcagcatcg aggccgagag ccccagatcg cgcgccgtgc gcagcaccag ggaatgcttc 35340
tcgggccaga tgagggtcag ctgcgcctcg cgatgcgcct ctacgtaggc gcagcgagcg 35400
gcggtgtcct cgcaagccag caactcgcgg aaagccagca gcgaacgtag gtagcggccg 35460
cgagcggagg cgcgcgagcg gcggcacagc tcggcccgat gatcgggatg caccaagggc 35520
acgttgggtt gcagacgcgc gcagatggat tcgtgcaccg ggtcgcagcg gatcatgccc 35580
ttggcaaaaa atccggccag atccgaggcc aactcgtaca ggcagtcctc ttgcgcgtcg 35640
taggcgaaca cggcgccgta cgcgtccacg aacacctggt accggcaggt ggcgtgcgag 35700
accgtgccaa tgagatgcag agctcggaat tcgccgaaaa agtcgttctg gcagtgctcc 35760
agatcgatct cggtcagcga gtgcggcgaa tgctcgcccc cgaccacgta gatgcactgc 35820
gagggccagc ccagcgacac gcacgagccc tcgaagcgcc gcaagtaacg ccgcaggccc 35880
tcatagtcgc gtcgcacgca caggtcggcc aagtcgcgcg tgcaaaagac ctcgggtacc 35940
aagcagcgtt tgcgacgcgg ccgacgcgcg tgcccgggca gaggaggaag gcgcgacggc 36000
ggcgacgacg aggaggaaga cgccgtggcc gccgagcagc ccttgcgacg gccggacatg 36060
ccggcagtcc gcgacgatcc acaggagaca aaaaagcaga agcagcagta gcctcggcga 36120
cccgctccac cccgtcctcc acacgctcag ccgcgactga acgccggggc gcgccgctac 36180
ttgggttttt atagccatct gccccccgtc tcgggcaccc gggagcgatc tacggagacc 36240
tgacagcagt tgggcaacac aagataggga aatacaaaga cacttttaat aaaaaacgag 36300
actactttgt gtgtgtgctc cgtaaactgt ttattctccc cctccgcttc gctctggatg 36360
ggctccgggc ccgtcaacac gcgactcgcg cggcaaaagg cacgctgttg acggcgcgag 36420
agcccgtcgt gatagtccat catgccccgg agatcgtgca caaagcagct gtcgccgcgc 36480
agaaaccgac gcagcgtctc cacgtgctgc agctgccggc gcgtatcagg agccgtcatc 36540
gctgatgtcg tcatcgccct gacaggcgcg tagatggctc cgcgagatca cgcgcgtttt 36600
caaccgccgt gacacatcag gtccatcttg agctggcgcc gggcctcgcg caggtgtcgc 36660
acgcgttgtg agcgggaggc gagttcggct tcttgctcga actcctgctg ctcactgtcc 36720
gagagggtgc gataaaaggc ggcaaagtcc tccaagtcgg ctacatgcgc cctgggtctg 36780
acgctccaaa gcgtacgcag tctgatgaag cggacccatc gagcgtcacg gcacgccgtc 36840
ttgaacgcgg ggcccgggaa gaggttcttc tccccggcgc gctcgggccg gcgaggccga 36900
cgcggtttat atacaccgtc tcggacggcg ggacgccgag cccgcgccgc ggccgctcat 36960
ccggagacgg cggaaaccgc ggcgccggag gaaacgggga ccggcaacga cggcggtggc 37020
ggcgaccaga ttatggggga aaaacccacg cttgtaaccc tgttgaccgt cgccgtgtcg 37080
tcgccgccac cgtcgtcgcc gctgccgctt gtcagcttca cggagctgct gttaccgccg 37140
ccgtccgtcg ccgccgccgc ggtggcggcg acagcgacga gcgaggtggg cgagaaaacc 37200
gcggagcaag aggtagcggc tgcgggtccg gagaccggga atgagagaag agaaaacagg 37260
gagaacgaag gaggggagac gaggacgaca gacaccaccg cggtcaaaag gtcgcacgac 37320
ggtatccctc gccaactagc agagcgcctg cggctgtgcc gccacatgga ccccgagcag 37380
gactatcgtc tgccggcgca ggacgtggtg acctcgtgga tcgaagcgct acgcgacgcg 37440
gaccgcgata actacggtcg ctgcgtgcgc cacgctaaga ttcaccgttc ggcctcgcac 37500
ctgacggcct acgaatcgta cttggtgtcc atcaccgagc agtacaacac ggcctcgaac 37560
gtgacggaga aagcttcgta cgtgcagggc tgcatctttc tctcgtttcc cgtcatttac 37620
aacaacacgc agggctgcgg ctacaagtac gactggtcca acgtggtgac gcccaaggcg 37680
gcgtacgccg agcttttctt tctgctctgc tccaccagcg agagctccgt ggtgctgcaa 37740
ccgctcatca ccaagggcgg gctctgctcg tccatggcgg tttacgacga ggaaaccatg 37800
cggcagtcgc aggcggtgca gatcggtttt ctgcacacac aactggtcat ggtgcccttc 37860
gtgccgcacg cctgcccgca ttacgccgtg cctttcacga cgccgggaaa gccgggctgc 37920
ggcggtgctc cgagcggcgt tgcggggttg gaggaggcgg cgccctttgg acgggtcagc 37980
gtcacgcggc atggcgcgac gctgctgtgt cgcgtggacc atctgacctg gatcagtaag 38040
cgcgtaacca cgtacggaca caaaaaaatt acgcgctacc tcgcgcagtt ccgcggcacg 38100
atggacgacg acgaggcggc gctacccggt gaggacgaag cgtggatcgc gtccaaaaac 38160
gtgcagtacg aattcatggg tctcattttc accgtcaacg tggattcact atgcgtggac 38220
gcggaacagc gccaactgct gggcaccgtg gccacctcct tctgtcaccg cgtctcggac 38280
aagatcacgg cgcgcaacat gccgcgcgcc ttttccttct acctgctgac gagcgcgcag 38340
cgcgggtacg acctgcgatt tagccgcaac ccgtcactct tttttagcgg cgacgcgctc 38400
aactgtccgc ttctcaatga gcccaacgtg ttttcgctca cggtgcacgc gccttacgat 38460
atccacttcg gggtgcaacc gcggcagacg gtggagttgg acttgcgcta cgtgcagatc 38520
acagaccggt gtttcttggt ggccaacttg ccacacgagg acgcctttta cacggggctc 38580
agcgtgtggc gcggtggcga gccgctcaaa gtcacgctgt ggacgcgcac gcgttccatc 38640
gtgatcccgc agggcacccc catcgccacg ttgtatcaaa tcaccgaggg cgacggtaac 38700
gtgtactcgt acaaccacca cacggtgttt cggcagatgc acgccgccgg agcaaccacg 38760
ttctttctgg gcgacatgca attgcccgcg gacaactttc tcacgtctcc ccatccctga 38820
ccctccgtcc gtcctccttt cccgacacgt cactatccga tggtttcatt aaaaagtacg 38880
tctgcgtgtg tgtttcttaa ttattcctcc gtgttcttaa tcttctcgat cttttggagg 38940
atgttctgca cggcgtccga cggcgttttg gcgcccccca tgccggcaga acccggttgc 39000
ggccccgtac cgctcttctg gggcgacgat aggtcgaaag ccaccgtttt catgcccgtc 39060
gtgctcttga cgggggaacc tacggcggcg gttcccgtcg agcggcgtga ttgcaaagcc 39120
gcgctcgccc ccggtttcag gatggaggga gaggccacag gcggcgcatt cgatacgctg 39180
cttttggccg tagacgacgg tgggtaaacg gtggttaccg cgggatacgt cggcgtggtc 39240
gaggcggccc ggctggtgcc ggacaggcga cccggcgcgc taccgctcac ggggaccgag 39300
ggcggtcgac ctaccaccgc tttgccgccc aaagtaggtt tcaaggaagg aacaacaccg 39360
acacggccgc cccggccttt caccggagac gggggggcac tcttggccgg ggacggagag 39420
gctgacgaaa gcatggacag cggcgatgtg gcgggggaca cgacatcatc ctccgtgggc 39480
gacaaaacgg acgccgaagc tgacggctgt cgagccgaag cggaagaggt tcccgcgcca 39540
gaagtcacgt tccttgatga cgtcgtttta gacgaagccg gttgaggttg caacagcgtg 39600
gcgggtaccg tcgacggcgt gcccgacacc tgtttctcta gccttccctg aaccggtgtc 39660
gacgtcaccg tctgcgctcg ggcggacgcg tgcggcgtcg cgactcgctt gcccagcacc 39720
ggtttctggc tcgtggatgt cgtcgtcatt ggagacgata acttagcttt acgtattctg 39780
gacggcgtcg actgctcggg cgtctgactg ggaggcgaaa tgacgtcgtt gtaatcggac 39840
gacggtgttg tgtgtcccag gctgacgacg gagccggtgt ccgaggagtc gtcgtcttcc 39900
tcctcgctgt cttcgaccgg tgactctgca gtttggtccc ttaaagccca aacctcatca 39960
gcggcgttct gagacgctgt ttgtgtcacc gcggcgcgtg gagtcgacgg cctccgaggg 40020
gtggtggaca cggtgttttg agaagccgtg gaagtcgtag gcatcctgaa gggattgtga 40080
gccaggtgag gattcttgag ggcccacgcg cgttcgcgcg gccagttggc ggggttcata 40140
tccccgggca acggcgccgt cggagcccag ggcgagttac cgttgaccgg ggtttgggta 40200
cccgcgaagg taggtgtcgg ggccggagcg ggggccgtgg aaggattgac aggcgtcggc 40260
gtgaggatgg cagtgccggc gccagcaggg acgttaactc cggcgccgaa cgtcaacgtc 40320
ggttgctcga acttgtacgc ggtggtgacg ggcggtttgg cactcgtctc ggtatccgtg 40380
atgtccacca gcgtgtcggt gaaacgcgga tcttgacggt tggggggata gccatccgag 40440
ctgtcggaat cctcgtcgcc cgagaaaaga tcccctctgg tctccgtgag cggcctcacg 40500
tcccacgcgc tgtcccgacg gacccttccc gggctggcct tggtcacctg cggggagacg 40560
agactgaaag ccgcgtgacg ctgttgttgc tgcgggatgt tcaagggacc gctggtcggt 40620
ttctgactgc ccgaggataa caggccgctg aaaacgctgg aaacaccgcc accactagcg 40680
acgcccttgc cgctagttcc cggtttcttg atgggcgtaa agatgttttt ctcgtcatcg 40740
tcatcgtcgt cgtcctcatc ggcactggag ccaaagagcc tccgggaggc gctcggttta 40800
cgtgccgggg gcggtggttg ctgttgacgt tgctgcaggt tctgctgcct ctcctcccaa 40860
gccttcagct gctgtttctc acgctgcacc acctcgtcgt ccacccgttt ctgccgctcg 40920
cgacgctttt cctcttcgtc gtaatagccg acggccgccg aacgggcggc gtgggcgtcg 40980
gcggccggtg ccagagaacc atgggcctcg aagcggaacg gtttgtgtcc cttccaggga 41040
ctggcgatcc agctccagcc gtccagcggc tgcgtgggga catgtttctt gggtaccgac 41100
gagaaggccg aaccgccgcc gagcgagagg agattggcgt catcatcaaa ctccaacgac 41160
ggcgagcgcg cgcccaaaaa ggtgtgcgcc gactgcggga agctgtccac gtagatgtca 41220
aagtcctcga tgagcagctc cagcagcgtg tcggccgagt cgccgttttc cacggcgtgc 41280
ttgaggatat tgcgacagta gttggaatca aaggaaaggc acatacgcag ctccttgacc 41340
agcagcttgc agcgctcctg aatgcgcgcc agacatttgc gctccagctc ctcccaagac 41400
ctacgcacgt tcatgatgag acggcccgtg tacacgagct tgttgacggc gttgaccagc 41460
gccgtgttgg cgtgccggtc caggttaagg tcgagcggtt tcacacagaa catgttacgg 41520
cgcacaccct ccaggttttc ttcaatgcgc tgcacctccg tatccttgag gtgcacaaag 41580
gcgatgggtt ccgtctggcc gatggctgtg accagcgtct cgcgcaccga catcttggcc 41640
agaatgaccg cgcttacgag cgcgcgctcg acgatctcgg catcgtggcg cacgtccgta 41700
tcgaattcgg tatggtctag cacagccagg tgatcgcgcg ccttaccacg atcaccgaac 41760
gggtaagtgt agccgcgacg cgccacggcc gcgcaacgca cctcgaactc ctcgagcacc 41820
gaggagaggt cggggttgtg gaaacgcagc tcgcggtagt atcccaacca aagcatgagc 41880
tcgttgaaca gcaccgtacg ccggtgcagg cgtttttcgc cacatttttt caggatcttg 41940
gggtgtgcct cgagatccac gtcgggcttt tgcgtgagat ggcgcagaaa gttgaccagg 42000
gctaccacat cgcgccgctg tagaccgata aactgcaaac tcatgctggc ttttctccag 42060
aacccggaag cgtcgtcgcc ccggactgcg cccgcggtct gctattcgcc cgcgatggac 42120
accatcatcc acaactcggt gagcgtccca cccaaaggga gggggggtag tttaatagcg 42180
gaggcggata cgcggttttc ttttaagcgc cgctgacttg tttcttctgt tttttcgccc 42240
cgtgtgctgt tccgcccaga cccgcaacaa cactcctccg cacatcaatg acacttgcaa 42300
catgacaggg ccgctattcg ccattcgaac caccgaagcc gtactcaaca cattcatcat 42360
cttcgtgggc ggtccactta acgccatagt gttgatcacg cagctgctca cgaatcgcgt 42420
gcttggctat tcgacgccca ccatttacat gaccaacctc tactctacta attttctcac 42480
gcttactgtg ctacccttta tcgtactcag caaccagtgg ctgttgccgg ccggcgtggc 42540
ctcgtgtaaa tttctatcgg tgatctacta ctcaagctgc acagtgggct ttgccaccgt 42600
agctttgatc gccgccgatc gttatcgcgt ccttcataaa cgaacatacg cacgccaatc 42660
ataccgttca acctatatga ttttgctatt gacatggctc gctggactaa ttttttccgt 42720
gcccgcagct gtttacacca cggtggtgat gcatcacgat gccaacgata ccaataatac 42780
taatgggcac gccacctgtg tactgtactt cgtagctgaa gaagtgcaca cagtgctgct 42840
ttcgtggaaa gtgctgctga cgctggtatg gggtgccgca cccgtgataa tgatgacgtg 42900
gttctacgca ttcttctact caaccgtaca gcgcacgtca cagaaacaaa ggagtcgtac 42960
cttaaccttt gttagcgtgc tactcatctc cttcgtggcg ctacagactc cctacgtctc 43020
tctcatgatc ttcaacagtt atgccacaac cgcctggccc atgcagtgtg aacacctcac 43080
actgcgacgc accattggca cgctggcgcg tgtggtgccc cacctacact gcctcattaa 43140
tcccatcctg tacgcactgc tgggtcatga ctttctgcag cgcatgcggc agtgtttccg 43200
cggccagttg ctggaccgcc gcgctttcct gagatcgcag cagaatcagc gagctacagc 43260
ggagacaaat ctagcggctg gcaacaattc acaatcagtg gctacgtcat tagaccccaa 43320
tagcaaaaac tgcaatcagc acgccaaacg cagcgtgtct tttaactttc ccagcggtac 43380
gtggaaaggc ggccagaaaa ccgcgtccaa cgacacatcc acaaaaatcc cccatcgact 43440
ctcacaatcg catcataacc tcagcggggt atgagctttc ctgttacttt attcagaaag 43500
caccagaacc cgtcgccatt tcccctcata tacggtacac gtccccctga tctgtcatca 43560
cggtacacag atttcgcccg actgcggacg ccgacggcca atcgcgtggc gtaggagtgg 43620
cgccccggct tcattataac gccacgtcgg agcccctgcg cgccacaacg ccgtccggcg 43680
caacttctgt ctcggcacgg tacgataaaa acgacgtccc ccgtcgacgt tgttttctcc 43740
gagcggtgat cgttcccgtc cctatcctcc ctccgcggcc cccacggcgg cggcctgctc 43800
gcacggacct atactattac cgccccaccg ccgtcgtcgt catgaacttc atcatcacca 43860
cccgagactt ctccaacgac gattcagtcc tgcgagccgc cgagatgcgt gacaacgtgg 43920
caggctcgat ttccaaagcg tacaagggca cggtacgcgc cgaaggcaag aagaagctgc 43980
tgctgaagca cttgcccgtg ccgcccggcg gctgctcgcg ccgcaacagc aacctcttcg 44040
ttttctgcac cgaacgcgac taccgcaagt tccaccaggg catcgcacag ctcaagcgcg 44100
cgccggccga actggacccc cacgagatcc agcaagtcac ggccagtatc cgctgccgcc 44160
tgcagcccag tctccgcgag ccgcccacgc cggccgacga gctgcagacg gctgtgtcgc 44220
gcgtgtgcgc gctcttcaac cagctggttt tcacggccca gctgcgccac tactgcgagc 44280
accaggacaa ggtggtgagc tacgcgcgcg acgagttgac caaacgctgc ggcgaaaaat 44340
cggcgctggg cgtggaggtg catcaactgg tagccttgct gccacacgag cgccaccgcg 44400
aactgtgcca cgtcctcatc ggcttgttgc accagacgcc gcacatgtgg gcgcgctcca 44460
tccgtctcat cggacacctg cgccactacc tgcagaacag cttcctacac ctgttgatga 44520
actcaggttt ggatatcgcg caagtcttcg acggctgtta ccacagcgag gcctaccgca 44580
tgctcttcca gatcggtcat acggactcgg tgtcggcggc cctggaactc tcacacagcg 44640
cggcggccgg gctgcccgag gccgatgaga acaacgacga gggagaggag gacgacgacg 44700
agctccgtca cagcgacccg gcgccgcttc acgagtccaa gaagccccgc aacgcccgtc 44760
gtccccgcac acgcatgccg cctcacgagc aaaagcccga agaaaacgag gaggaagaag 44820
aggagctgtt tccctcctgc aaggcaaccg cagcattcct gcgggcagaa ccctccgtct 44880
ccaacgacga cggcaacggc ggcgaacgct gcgacacgct agcgaccgcc ctgcggcatt 44940
gcgccgacga agaagacgga cctctagcca gccagaccgc tgtgcgggtc gccgcgaccc 45000
cctcaccttc agtcacccca gcccttaccc ccgtcacgtc ccccataacc ccgttgtgta 45060
tttaacgtca ctggagaaca ataaagcgtt gatttctcaa gttccgctct ggttttggtt 45120
tcgttttcaa agggagcccc atcatggccc aaggatcgcg agccccatcg ggcccgccac 45180
tgcccgttct ccccgtggac gactggctca actttcgggt tgacctgttt ggggacgagc 45240
accggcgcct gctgctcgaa atgttgaccc agggctgctc caactttgtg gggctgctca 45300
acttcggcgt gcccagcccc gtatacgcgc tggaggccct ggtggacttc caggtgcgca 45360
acgcttttat gaaggtaaag cccgtggccc aggagattat ccgtatctgc atcctcgcta 45420
accactaccg caacagccgc gacgtgttgc gggacctgcg cacgcagctc gacgtgctgt 45480
actcggagcc gcttaagacg cggctgctta gagggctcat ccggctctgc cgcgctgcgc 45540
aaaccggcgt caagcccgag gacatcagcg tgcacctggg cgccgacgat gtgacattcg 45600
gcgtgctaaa acgagcgctg gtccggctgc accgggtacg cgacgcgctg gggctgcgcg 45660
cgtctcccga ggccgaggcg cgctatccgc gcctcaccac ctacaacctg ctgttccacc 45720
caccgccctt caccacggtc gaggcggtgg atctgtgcgc cgagaacctg tccgacgtaa 45780
cacaacgtcg caaccgaccg ctgcgctgcc tcacctccat caaacgcccg ggctcacgca 45840
ccctggagga cgcactaaac gatatgtatc tgttgttgac gctgcgacac ttgcagctgc 45900
gacacgcgct ggagctacaa atgatgcagg actgggtagt ggaacgctgc aaccggcttt 45960
gcgacgcgct ttacttttgt tacacgcaag cccccgagac gcggcagact ttcgtcacgc 46020
tggtgcgtgg gctggaactt gcgcggcaac acagcagtcc ggccttccag ccgatgctgt 46080
acaatctgtt gcagctactg acgcaactgc acgaggccaa cgtgtacctc tgcccgggat 46140
atttacattt cagcgcgtac aagctgctga aaaagatcca atcggtctcg gacgcccgcg 46200
agcgcggcga gttcggggac gaggacgaag agcaggagaa cgacggcgag ccgcgcgagg 46260
cccagctcga tctcgaagcc gatcccacgg cgcgcgaggg cgagcttttt ttcttctcca 46320
agaacctgta cggcaacggt gaggttttcc gcgtgccaga acagcccagc cgctacctgc 46380
gccgacgtat gttcgtggaa cggcccgaaa ccctgcagat cttctataac ttccacgaag 46440
gcaagatcac caccgagacg tatcacctcc agcgcatcta tagcatgatg atcgagggcg 46500
cctctcggca gacgggcctg acacccaagc gcttcatgga actcctcgac agagcgcctc 46560
tgggccagga gtcggaaccc gagatcacag aacatcgcga tttatttgcc gatgtttttc 46620
gccgtcctgt gaccgacgcg gcttcttcgt cgtccgcgtc ttcgtcgtcg tcctcagcat 46680
ctccgaattc tgtttcgctg ccgtctgcca ggtcgtcatc cacacgaacc accacgcccg 46740
cgtccacgta cacctcggcc gggacttctt ctaccacggg tctcttgctc tcctcttctt 46800
ccttgtcggg atcgcacggc attagctccg cggacctgga gcagccgccc cggcaacgac 46860
gccgcatggt cagcgtgacc ctcttttcgc cctactcggt agcctacagc caccaccgac 46920
gtcaccgaag acgacgcagc ccgccacccg caccccgagg gccggcccac acacgcttcc 46980
agggacccga cagcatgccg agcactagct acggcagcga cgtcgaagac ccgcgggacg 47040
atctggccga aaacctacgg catctctgaa cgcggttttt cctctttttc tacgtgtctg 47100
tctcaggacg agacgtcgat atcaataaaa ataccgtcga cgtggttttt ttaacagtgt 47160
ggttttcttt attgactagc ggagtacaca gtttacgagt aaaaaagaca gggaaaggtt 47220
atataaaatg ctgtattata tacaaaaaca tgcacataaa cagacgggac caccgtgctc 47280
gtcatcctct cctcaatcag ttgttcatgt aggcgtgtgg cggggtgagg ggcggcatgc 47340
cgttggcggc gccgggaata atgtgccgtc gaccgacgtc gcacaccttg aaacgccgtc 47400
ggcgcacgca gcggtcgcag gacgggatat cccagaggaa gcccatgtag gtctcggggt 47460
cctcgtcgtg aaagcggtag gagagttcaa agtggtgcaa cgagcccgtc cgagctcgca 47520
gcttctggcg aacaccctcc acgtcatcgg tgcacaacga cagtgctggg ctctcacaca 47580
gggcctgaag ctcctgcggc cacaggtgcg tggccagggg cgagtccgtc gtcaccagtt 47640
tgacgcagtg catcaggttc tcggtgatgg cgtcgtacag gcgactctca gcctcctcgt 47700
gcgtcatcac gtttcgaggc agcgacagct cgtcgtcgtc atcctcgtca aacatgatca 47760
tggggtcagg ggtttttttg ggatgttgac aggtgggtgt cttttccaga cgcacgatgg 47820
cctcacgccg gccgctgaaa cggtggtttc ggtgtccctt ctttcccatg acgcaggtga 47880
acataaccac gtcctcggcc aaacggtaga cggcgtccat ggcggggtcg tagccgtaga 47940
cgacgccgaa agtgtccacc aagacgtact ggcgtacgag gaactctttg cgttctggca 48000
cctcgtggcc cagcgcgccc aacaactggt ggtaacaggt gatgcgcggc acggtacgga 48060
tcatgagctc catggtctgg atgctgccgc ccgcgcggac gacgctgaag gatgtttcct 48120
tgaacttcat aacctctgtg ttgtgggtcc agaaggcgaa atgggtgtcg ggacactcat 48180
cgaaagggtc gtcgatggtg taggaagcgt agccccgctt ggtcacctcg gccgacaggc 48240
tctccacgtc accgcggtag agcatgacgg cgttccagta atcgtcgtac tgcaccatgg 48300
gccgctggta gtcgcgcata gtgtggaagt ggtcgcagtg acgaaagcca tgccgcagaa 48360
agtccttcat ggtggatgcc agctcgtaga cgcagtcgcg caggtcatcg tagcagtaga 48420
tgccgccgcg ctgcccgatg agcacgatga gttggtagcg cataaagccc ggaccctcga 48480
cgaagccaaa ggggtgcagg tattcctgac agcacacgta agcacctggt ggagaaataa 48540
gaaaaatcca cgcacgttga aaacacctgg aaagaacgtg cccgagcgaa cgtcctcttt 48600
ccaggtgtct tcaacgacgt ggggcttacc ttgcgaacag acggtgccca tcttgcccac 48660
gaagggcccc agggcgttgc gcgaacggag ctggatgaag cagcgttcgg gccaggccac 48720
gtgcagccgg gtgccgcatt cctgctccag aaagtcgttg agaccgttaa agtccccggc 48780
tcgaatggcg atgcagccgt aggccatcag cgtgtcccgt aggtcgtcca tgacggactc 48840
ctctaccttc gctcgccgac gctgcgcttc tccagccacc gctgcggtcg acagactcct 48900
tcgtccgcct tcggagaact acggcgcggc ggcacggcct ttatagacac tatcagcgtt 48960
gacgtcagac gatccgatga acgtcgtttt ttgtgctgga acttccctcg tcccgacaaa 49020
tgtagcggaa atcttcaagc aaatcgcgac gaagtccgat gaggaggatg caaaagaggc 49080
tgagcaacgc gatgctgccc gccgccacag tacatatgct caacaacgcc cagtgtccca 49140
acgcgcgact tttggctcgg agcagagccg aacggcggtt tctccacatg acagataacg 49200
tggtccagta cgtccatcct ttgcattccg gcgtccagac gggaagcgtt gtcatgttag 49260
ttcccgtaaa ggtcgtgttt tgtcttgttt tgtttctcat gagtttaaca gtccttttta 49320
gaaaccgcgg gcacatgtct tgtagaaaga tgtaatcact ctccgcgtat gtcgctaggg 49380
ttgacatcac agtggtagtg ttttccgaag aagtgacgtt gtcagtgata ttgtcagtga 49440
cgttaatttc ttcccagtgt acggataact cgaacggtgt cgtatgcgcc accgctctca 49500
acacgtaact acggccggtg aggttaagtg ttagttgtcc cacggtcaca ttggtgtcat 49560
ttgtaaaaca cgcgatttct ccgcgaactt ccgtgacgtt ggtttcacgg gtctcgttga 49620
gaacacgcag aggaaaccag ccttccagat gatactggaa accaaacgta agcatgacgc 49680
tatgccattg tctccgtggt tgccgaaacg ttacgttcag aggcagtttg gcttcggctc 49740
ctgcgcaagg cccgttatag atttgcgtgt cattgcgcgt acagtttaac cggcagttca 49800
tactcgtggt gttagaagtg atgttaacac ccgtgccgtg gtacgtgcat cggaccgaaa 49860
caccgtgtcc cgtgctccaa aacagcgtca acaacagcca cacagacacc tacgtggaga 49920
cgacacggga ctttttattg acggagactc acgtttctac cctccccttt cccgtaggta 49980
aaaacccacg tttatcacac acgttgtttt tacctgaaac ccgcgcagcc cgtggacgcg 50040
acaaaaaacc gcggcactag aaagaaaatg aaacaagtat gtttattaag cagcatgtgg 50100
ggctaatagg ggggataact gaggtatagc aactatgaaa aaatactaca aaaaaaaaag 50160
ctgaacatgg tcatctagca gcaaagttct ccttctagac cacgaccacc atctgtacca 50220
cgtcgccctc cccggtcgtg tacatcacat ccttcaccac gaccggtggc aacggcggcg 50280
acgaggacaa ctcgctctcg acggaggccg ggacgacaga ggacgggggg gtggtggcgg 50340
cggaggacgg aggggtggcg gcgacagcgg ggtcttcttc cgacacgggc gacggcaggc 50400
tcggcggcgc ggacagcacc cgttgcgccg gggcgtgaga aggctgagcc ccggtggcct 50460
ggatgtgggc caacgaattg gctcgcagcg agtcgcgatc cacgaaggtc ataggaattt 50520
tcccttcgcg gatccgccgc tcagattcca ggatggcgcg cacgtagctg ttcaccgact 50580
tggcaaaagt gcgcggccct tccgtattct tgtcgcgacg cgcttccagc acctgctttt 50640
cgtagtccag ctggtggaag accatcacca ggtcgtccat agtgtgcgcg tgctgacgga 50700
cgtgggagcg cacctccacc gggaacaaag cgttccaata ctccagcacg atggcaccgt 50760
gccagaactg cgccatgctg ggcgccagga aaaacaggat accggagtcg taggcgaaca 50820
cgtcccactt gggcgtcatg aacaacacca gctgacgcgt gggccgcacc gaagcttcct 50880
cccaggcctc gatgaccccg aacatgatga gctcctggtc caacgggggg cagtgtcgct 50940
ccagccaact gatcttgctc aggttcatct gcagaaactc gtaagagggg tcgcagatgc 51000
acacgtagag acccgagtcg tgccgcagcc tggctccgcg cttcatcagt ttcctcaccg 51060
cgtagcgaag cgccaccttg cccaacgccg acgcctggat cagtcccccc acgtccatct 51120
gcgtctgtcg ccactcggcc tcgtccagca ggctcgtgat agcggaagtg ctatgcgtgg 51180
tcgtagtcat cctttctatc cttctctatg aatagcagca atagcggtaa agtcccttct 51240
tatactatcc cggagtctgt ggtttttttt gtttacccct gcttactggt gagactgctg 51300
ggggccgttg tgctgcagca gctgagctcg tcgccgccgt tgccacagga accggtgcct 51360
ccgcagggcc tttttgaggg cctcgcaggc ttctcgcgca agtcctgaga ggccctcggc 51420
gtcgatgggg ttcacctcgg gcgtccgagc ctcgttttct tcttcttcat cctccctttc 51480
ctcctccgtg tcctcccgct ctgtgtcctc cgttacgctc tcctccccgg cctcggccaa 51540
gagcgcagcc accaagtcca cggaccgctc ggtctccgag ttctcaccgt caattacgcc 51600
atgttggcgg cgtaaccggt gccgagaacg ccgggtgagc gcacatgctt ttttctttct 51660
taaccaaggc gggagaggat cttcaaggcg ttttcgctgg atccagcggt agctaaagta 51720
ccaaaaggcc agcaggccca cgctacctaa cagattcacg tagactggag acataattaa 51780
agaaagaagt gaaacccgcg tgtgggtctc acgtcgtctt gaaacaccgt cttatataca 51840
tgaagatgcc ggacatgacg cgcccaagac acgtggggtt tcccccttag gggacccggt 51900
ttcttaagat gtttttcatc ttcgcacgcg atgtactaca tcaaagggtc ggctgaccga 51960
ccgcattgac gcacagtttc cgagtacgcg cgtctcggag cacctgacgg tgagccaccc 52020
agctcacgcg gataggggac aacactgacg tgaggggcga ttcacgtcac tgacggctga 52080
cgggaataag acgggtgagg gatttccacc tttttcttaa gtgtgactct ccttacggta 52140
aatcgcacct gtgacctctt aacccctcct ccctggtacc caataacagt gaaaaacaca 52200
caccacacgt cacgacaccg atcgattttc tttattctta gtgtgatgat aggtaagggc 52260
actcgtgagg atgtgcagtt atcattatca agccttcttc aaggcgtagt gatgatcgtt 52320
gggcagaacc cccaagctcc tagcgatctg ggaatagaag gaggagaacg accccagggc 52380
cagaatgccc acagtgtaca tggcccaggt ctccagaccg aacgtggcgg gtcgcagctt 52440
cagatggtag gccacccgct ccgagagttg tgaatgctcg ttcaggcaac aggactgcag 52500
gtgggtgagc ccaaaagcgc tttcgtttac gccgcgcacg tgcaccgtct gggccgggca 52560
atcctggtgt tgcgcgcgaa aatggtcctg acaggagatt ccgtctacgt ggcggcgcgt 52620
gttgttaccc acttcgatca gcaacgtgtt atcggcagga tgatgcgaga acgcgacgac 52680
ggtgttgctg gaggtctggc ggcagcagta cacgtcgagc gtcatgaggg ccatgtcgcc 52740
ttggtggtac acggcgtacg cccaaccctg gaacacgagc ggacatcgcg gaccgtgagc 52800
ggacatcgcg ccggcggttg ttaccgtcgt ctcggcagga gaacacaata aactcctgat 52860
cctcatacac aggagtccaa gcgtcagaat taaagtccgc ggagccataa ccgcgcaagt 52920
gaagccgata cgagtgttgc tgaatttgtt cattctgccg actgttgctc acgagcgttc 52980
ggaggcggtg ccacaggctg ttggccatta aaaagtcctg gcccgaatga cgacgagaca 53040
gagcccgagg cgaagaaaaa ggcgcccgtc atgaagacgt aggcagggga attcccatat 53100
ttttatggct tcttttaaaa gtctgtatcc gactccatcc ggcgcttttc ccaaaccgtg 53160
gtctcctcgt cgtccgactc ggtacccagg aggtggtaag tcttttgccg cacgtagaaa 53220
gctttcaacg tggagcaaaa aatgagaata aagaccccga aaacgaaaca aaccacgccg 53280
atcatgccga tgcagacgtt catgtcgacg tagccggcgg tgctgttggc ggtgcggcaa 53340
aagagtgtca tgtcgtgcgt gcacaaaaaa caacacacac cacaggccag gtcgtagcgt 53400
agttattatt ccgtagcagc aatgatggta cagtcaagca catgctctat ttcccgttac 53460
cccgatgatg atgatgatgt tgtccccgtt gcagtggaat tgtcccggtt aatcaccacg 53520
gtgaacacca cggccaagaa aatgatccct aatatagcga ccactaagag agcaaaagtc 53580
catttccagc cgttgtcaaa gtacgccccc gtggtgggat gcatggtggc gggcatttcc 53640
atcatatcca tgtcgaacgt gtgtcgcggc gacggcgaac taaccaggca gtacgggggt 53700
cgatagggcg gtgggctgca gtcgggtggt ggcggcggtg gcgtggaaac cgtcgtcggg 53760
cacagaccca tggcctgctc gtaggtgggg ggcgcgtcgt cgtgatcccg gtcgcggagc 53820
atcggcgtgg gctccatgtc ggtggcagtg acggcgacgg tggtaactgt ggtggagacg 53880
gtaccgacgg cgtccgcggc tcaccttcga gcaaagagcc ccttcttttt gcgcaaacga 53940
cggcaaaaca gttctctggg acagccggtg gcgcggtaag cgggtgccac gctttcaggg 54000
tgggtaaaac agtcgcgggc gaagcagtag ttgttgcaga accgcaaaaa cccgacgcga 54060
aagaagccca ggagtccgcg cgccagaaag tgcgcctgcc gcgtctcggg atgcacgccg 54120
aagacggcgc cgctctcgtt caccagtatg gagatgtcca ggcgctgctg cgactccacc 54180
ggcacggccc gcaccacaaa tacctgcagc acgttcagcg agcacgtctc ttttaaccag 54240
ttgccgtggg ccggatcctc gtaagtctgg ctcccgttca agacgaccgt cgtcagcgcc 54300
tcattaccgt ctcgccagct gaagatggaa ccctcgcgct tcatgcacag gcgccacagg 54360
gccagcaggt cgcgcgccaa catgaactcg cgacccacgt cgccgccggt ctcgaagcgg 54420
acatagccca gttcttcgcg cagcggcgcg tagttgcgca ggccctcctg cacgaagccg 54480
cggaaaccgg accgcgacac caggtacagc gattccacca cgggcgagta gacgtagacg 54540
cggccgccct cgccgatgag tacgggtagc ggtgggcggc cgatggcttc gcaacgactc 54600
acagtgccca ccggcagcag gaacttgtcg cagcacagga aggtcttctc caaaccttta 54660
atattgagat gtccaaagta gccgacgcgt aacaggtcgc agtaggtgaa aaaccaaccg 54720
ttcggccagc tgagacgcag caccgtgccg ctgacgcgac gaaccagctt ctgcaggtcc 54780
ttgcgggcgt cggcggtgac agagcagcgg aaggtctcgt tgaccagctc gacagccagc 54840
gcgtcctcca gcgtacgttc cttcatctcg tcgttgatgc tctggcggcg ccgccggatt 54900
tcgtcgaaac gagccgcgga ggcggcgacc gacgcggagg tcgtccgaac gccctctgtg 54960
acgctgtcgt ccggccagtc aagaaagcta aggctggcgc tgcgccgcct aaagtgtccg 55020
atccgcgcgg gacgtcgctg agggacggtg gctggtctgc tggggcgggt acggccgcgg 55080
gtgtccgcgg acacgttagt tatacacgga attgagtcac gtggcacgtt gccagctgaa 55140
accgccgtcg tctccgccgg cgttttctcc atcacgggac cgcgccgtgc gcgcgttccc 55200
aggcacgcgg cccacgctct acccgcactt ttgcttcttg gtgttaggga cgaactcgaa 55260
cgttacagaa tcctcgctgt cgctctcctc tttcgcgtcg ttaaagtaat tgccggagtt 55320
gcgatccaaa ccgccgcctc ctcctcctcc gccgccgccc gatccacctt tggacgtcag 55380
gtagctggtg atcttgtgct gctcgtattt ttccttggag gaaagaccgt ggtcgtgatc 55440
accgccgccg ccaccgctgc tcattttccg cgtaccggaa ccaccgccac caccgcggtc 55500
gtgcttcttg ccgccaccgc cgccacctcc tcccagaccg ccgagaccca tgggttcgtt 55560
catgagatcg ttatccagac ccgggccgtc gtcgtgcaga ccgccggcat tggccagcga 55620
agagaggctg ccgccaccac cgccgccgcc acgcgacttg ccgctgttcc cgacgtaatt 55680
tttatcgaag ggatcgccac gctggaaagg ttcctcggtg agaaaattct ccacggcgaa 55740
cagaccgttg cgactggcca cgtacaacag cgtgtcgtgc tccgtaacta tacgcaacgt 55800
gcacggcagt ttggtgacgg cgcaattgag cagcgtctgg tagaagttct tcagctgcac 55860
gttgatacgc atgtttttta cgccgtggaa actgacgcgg ttattggccg tgaattccag 55920
ctcgctgccg ttggtcagga tgaatttgat ggccggcgga ccggcgtgca ccagaatctg 55980
cacggtgccc gtagggcagg gcgctttttt aacgttacgc ttgacgcggg tatgcggccc 56040
gatccactta agcaggtcgg ccaccacgcc gaaatctaga tccacgtgca cggccgaatt 56100
ctcgctttcg cgcacaatgt cttggccgtg cacgcaggcc gagctgaact ccatattgaa 56160
atcgggcgcg cacatggaga tcttggccga aaggtccgag atgtcctgca cgtagaactt 56220
ggtcaggtcc ttgctggaag tcaggtacat gaaattaccg agcagcggcg tggaattgtt 56280
aatggtcttg ggctgaaacg acttgtcagt gatgtagaga catgagctgt taaaagtgat 56340
ttttgacacg cagtgactgc gtaccgtttg caagataagc gacggcgtgg gcaagaaggt 56400
aaccgtggtg ttctccttga gcgcacggat cacagatcgc agctgctgga tagccgtctt 56460
gtacggcttc agccgcagcg ccagcgtcgg cggctccgag aggcgcgtct tgcgatccat 56520
cccggacagc gtgcaagtct cgactaagga gcgggcgcga gcgagcgaaa gttttataga 56580
gagcacacac gacgaccggg aacgctgcga agacgcccgg cgtctaataa tacagccgcg 56640
ccgagccagc gggcccccga ctaagaggca cagtacttat atactccgac cttaaagcgc 56700
cagtggtacc acttgagcat cctggccaga agcacgtcgg gcgtcatccc cgagtcatag 56760
tagaaaacca gggccacgca ctggtccaca aacacgctca ggttcacggc cgccatttcc 56820
acgtcgtttt ggatcgccgg cgccgcctgg aacagacact gcgtcgcctt accctcctcc 56880
tggtgctgct ccaaccacgc gtaattcacc acgggcacgc gcagcggcct ccgcaccacg 56940
gtggggaagt aacactcacg gttgggcggg cacaatgacc acaccgtctc ctcctcgaac 57000
acggtgccgc gcgaagccca cactgacggc gtcacgcccc acagatgcgc cacctcgtcg 57060
tcgggaccca ccgccagaaa ctgacagttg cgcaatccga actcgagcat gtcggcgcgc 57120
agcgcttccc agcgcgcgct ggcgatagag agccgcggca accgatacaa ttcgaaaatg 57180
aatttgccct cttgatagat ggtgcgttcg aaccactcgc agcgcggcaa acccgacttg 57240
cacaaatcga cgctagcgcg caccgcggca aagtacatgt gctcaaagat gcgctcgatc 57300
aagtcccaag aggcaaagta cgtgaaccct aaccgcatga gcgccgtgtg caagccggcc 57360
acgccgatgt gcagcggacg cagtttttcc agcgcgctct ctacccacca ttcggacgct 57420
gacattagcg cgtccaggcg cgcgttgccc caaaccaccg cctcggtcac caactcgcgc 57480
agcacgctca aatcaaagta acgtcgcgtg ttccccaaaa ccacgtcggg tagatgcagc 57540
ttctgctcgt cgctacgcgc aaacacgcag cgagccacgt tcaccgtcag ccgctgcacc 57600
ggcatgtcac actcgccaaa gtggcacgac gccatatcgg gactcaagca cggcggcagg 57660
cacacgctgt cggccataat cgagtacttg actacgtgat ggacaaagac caccgaggca 57720
cggcccttga gcgcgcacag caacatcttt ttcagaaaat cgtccgtgtt cacgatcacc 57780
ttggggcacg attgctcgca gcgcgaatac tctttctcga aagccgactc ctgacccagg 57840
tccgagagcc gccgggagac aggccgcccg aacagcgagt agcgctgctc acgcgcacgg 57900
tatcgcttca ttaacacgct aggcacgttg aaagcgtagc aaacccccgt caactccgac 57960
gtgctttctt tgagaataaa gttaatcacg cggatagcgg ccacgtccca catgtccaca 58020
aacacacgta ccacgggtcg atgcacctcc ttctcgcgta tcaaatcgca gtatcccccc 58080
aggcaacgaa tcacgctgtt cacatcggcg ttaagtcgcg ttacgttcac cgacacagaa 58140
acgccgcaac tcaaggtgct catccatttg cacatagccg cccaactggc gtcacgcgaa 58200
aaagggtcgg ccgagatcag aaagtcgtac tgcggcacgc gatcgaaacc cacggtagac 58260
atggtgaagg tggacagcga cagctgccca tcgcgacagc gcttcaacac cgattccaac 58320
acctcgcctt cgaaacgcgc atccagatgg aaacgataga tgcgcgagtg cctactgttc 58380
tcgatagccg ccgtcaacgc cacggcgatg cgcaaaaaca cgccgcccgg actctcgtcc 58440
tgtccgtgca gttggcgaca caccttatcc aaacacaaaa tggccgcgta caagccccag 58500
caaccggcca attccacaaa acgcgccgtc tcctcggcca gcttgggtag atcctccatg 58560
tgacgcagca caaaacggcg caccgactca tcgcacagct ccgaagcgta acacagtggc 58620
gtgcggcttt cacgcgccca gttggctttg aaataaaagc gacccaacag cagatcgcaa 58680
cgcggcgagt gacgaattag acagggaccg tggcgcatga taagctgaaa cagcctgaaa 58740
ctgcccaaac cggcactgtg ccgcgacacg gtgtccatct cgcgccacag cgcgttcctg 58800
tcggacggca gctcccgtgc cggctcctgt acgccgcaaa agcgaaactt gccccaatag 58860
ccgtgacaat gacacttttt gcccatcaac atgcgcgtag cctgtatcgg cggcgatact 58920
ttgcagagcg aagccccgaa atcgtcctcc tcctcgacac tgtccagctc catcctggtc 58980
gcgccggtcg gattgaaggt gctcaaaccg ctactcacgc gtccaccgcg actgggcacg 59040
gcggaaccgc tgtcacgcgt caacgacagc acagacggcg tgccgtcagg agacggcgac 59100
tcgggacgcc aactgacgac gccgccacca ctcgtaaaac ccgctacaca cgctacgccg 59160
ctcgacatgt tagtattttc agcggatgct tccttgtcac ccccgggcag cggcccttcc 59220
tcgagctcgc tgtcatctcc cccggtagta tcagcgacgg cctctgccga cgattcctcc 59280
gtctcggttt ccgcgccgcg gctcggaatc ctacctggcc ggcaccgatg tgcgggcacc 59340
gaggacaccc gctgttcctc gtccgcgtca gccggagtca taagtttacg aggaaaagaa 59400
caaagaaatc aggtagattt caataaagtg agtctagatg gcgccgataa ctacggttta 59460
taaagtctgt gtgcgatgtg tttatttttt tcttctgtgt ctcctccccg tatgctgtca 59520
gcgccgctca gacgaattct cgaaagtctc ccaattcgac gctaaagttg tccaaacgga 59580
cgacggacag tttgagttct ttgtgtacca ggaacgaggt gtgaatgtcg tcagccaggc 59640
accagcccag cttttgtatg accccggtac acagagggat ctggcgtggg cgcgtgatgc 59700
gacggttgac aaagctacag cgctcgcggg cgaactttcc gcgtgcaacg tcgaccaggg 59760
tctgccagtg tgcgatgctg gaggtgagca cgtagatgcc gggacgtgtt tcgggcccgt 59820
catagtcata gacgatgatt aaatacacgt attgcagccg tccccgggtc tcttcccacg 59880
tcagatacat gtctttcggt atcatcaacg cgaacacctc cgttttgagc gtgttgtaaa 59940
ggtagccgcg catgacgcag gtgagcaacg aggtgatgcc cagcgagacg gtcttgacgc 60000
agcccagcgt ctcgaggcgg cggtgcagca gatgcgggcc cagatccagc cactgcagcg 60060
cggcgcgcgc ggccgaggcc gtgtacacgc tttcgagcag gcagcgcgtg ctggccgaga 60120
cgttggaggc gcgaatgcct aacaggtaga ggctaatgta gaggtgtcgc ggcgagtcgc 60180
aacccgtctc catgcggatg agcagcgcgc ccggctgcgc ctcgaactct accaggccct 60240
cgggcacgaa gaaacgcgcc gtgagcgcct ggtgatcggc gtggtagagg tagcgcaccg 60300
atatagtatt tacctcgcgt ttggctttga gcgccgtcac tagttcattg tcctcgtcgg 60360
ccgggtcgcg cggccgtttg gccaccgcgc gcgcgtccat gatggcgagg cgcacggtag 60420
atttcaaaaa gttgatagag cagctgcggg cacgggccac ggacaaagcg gaggcgttaa 60480
ataccgtgag ccaattggag atcggcgcgg tggatgccca ggacgtgacc gcgagcgccg 60540
tgcgcgcctt cgtgggtgcg ttgccgagct cgggctacca ctttggcttc gtgcgtcaga 60600
acgtggtctt ttacctccta agccacgcca cggtacagac ggcgcgcgac ccgctgtacg 60660
ccgccgagca gttgcacgaa cagctggacc gcttcctgcg acaccagcac gacggcggcg 60720
gggacgagga ccggttgccg ttctaccaca acggggccac actgacggct ttccagaagc 60780
tgttgcagac cctgcgcgag atccagaccg taatagccga acagagcggc ggcaccgcgg 60840
cagcggcgga cttgatcgcc agtaacaacg cgtcgaccga gcgccgcggc aagaagggcg 60900
gttcgagttc cgggggccag cagccgctgg tccgccgggt gatcacgcag ctggaaacgg 60960
ctgccacgga ggcgcggccc tacgtcaatt gtcgcgccgt ggccgaactc ctggacctga 61020
cctaccagcg gctcatctac tgggcctgca cgctcatgcc ctacgtgttg tttcggcgcg 61080
acaccgacac cgaactggac acggtgcttc tgatgcattt tttttacaca cactaccgtt 61140
cggttaacgg cgatttggcc gtggagtttc aaaactacgt caagaacagc gtgcggcaca 61200
tgagctcttt cgtcagttcc gatatcgacg gcgaccagaa gcccggtgcc gaacacatgc 61260
gtgacgtcag ctacaagctg ttcgtgggta atctgcaggc gcgtgacgcc agcggcctca 61320
tgtttcccat cattagcacg cgcatctcca ccgtgaacct ttacctgtcg cccgaacgta 61380
tgtttttcca cccgggtctg atctcgcgtc tgttgagtga ggaagtttcg ccacgcgcca 61440
acctagacgc ttacgcgcgc gtgtgcgatc gcgtgctgga agaccacttg catacgccgc 61500
gacgcgtgca gcggctactg gatctgacgc agatggtaac gcgactggtg gaactgggtt 61560
tcaatcacga tacctgcgcg gcctacgcac aaatggcgct gatccagccg gccagtcaga 61620
agagctcgct ctttgtcagc gagattcgcg agaaactcat acagatcatc tacaattttt 61680
acacgttttt catgtgcctc tatgtgtaca gccccacgtt cctgttcgac caccggcggc 61740
ggttgatttt ggagcagcat cgatccacgt tgatcggctc caaggaggaa ctacagcacg 61800
tctggagcaa cgtgatactg aacgtcaata cgcactttgc ggttcagtac acggaagaag 61860
actttgaggc acatacgaag ggtgccacgg aggcggagcg cgagtacctg tatcgggacc 61920
tgcacagcaa gtggggcgtg cacctgttta ccttgcgtcc gtctcgcggc gcggccggcg 61980
cggcctcgcc tttgcctccg cttgacggcg tcacacgctc cgacatctta cgcgaatgcg 62040
cgctcgttaa tctgaacgaa ggccgcgtca actacgcctc cctgctagcc ttcagtcatc 62100
atcccgagtt ccccagcatc ttcgcgcagt tggtggtggt aactgaattt tcggagatct 62160
ttggtatccc gcagggcctg tttcaagccg tgggttcgcc gcgtcttttt gcgctcattc 62220
agctgtgtcg tgtattgttg cccgagcagg tgacgctgta ccagaacctg gtctccatct 62280
acaacctgac cacctttgtc aagcacatcg acgccgcggt ttttaagacg gtacgcgatt 62340
gcgtcttcga catcgccacg accctcgagc acctcagcgg tgtacccgtc acgcccaatg 62400
tggacctgct ggccgagctc atggcgcgct ccgtagcgca taacctgtac accaccgtca 62460
acccgctgat cgaggacgtg atgcgcagca gcgccggcag tctgagaaac tatctgcgac 62520
acacgcgact ctgtttcggt ctggcgcgtg ggcgggcgcg cctctcggag gacggcgtga 62580
cggtgtacgt ggaggtacag ggtcagtacg gactgcgcgt acctaccacg cgtttcgtag 62640
aacagttgcg cgagctggtt cgccgcgatc ggctgttggc cgagaatctg cgcggcttga 62700
atgagcgcct gctgagtgtt cgcgtgcgcg tacgtcagat cagcagcgac acagaggaag 62760
taagccgaca cgccaagggt caccgcacgg tggcccagat gagcaaggcg ctcaaaaaga 62820
cggcctccaa aatcaaagtg ttggaaacac gcgtgacatt ggcgctcgag caggcgcaac 62880
gttccaatgg cgccgtcgtt accgcggtgc aacgcgcgct agccgtcttt gacgtactaa 62940
gtcgcgagaa cttggaacgc cgcggcgcac agctctgtct gacggaagcg acgagcctac 63000
tgcaccgaca tcgcgcgcta gcgccgatga cctggcccgc gggcacgggc gttgcggcgg 63060
cggccgaagc ggatcgcgcc ttacgcgagt tcttggaggc gccctgggaa tcggcgcccc 63120
aaccgccgcg actccgcatg acgcccgaca ccgatcacga agaatcgacg gcaggcgcga 63180
cgtccgtacc ggaggtcctg ggtgcgcgct acgaacccgc acacctggcc gcgagcgacc 63240
tattaaactg gtacatcgtc cccgtaagcc aggcgcagca ggacatcttg tcttcgatcg 63300
acccgcccgc cggctcgaca tcggtgtccc tgccgccggc ctcgccatga aagtcacgca 63360
ggccagctgc caccagggcg acatcgctcg ctttggagcg cgagcgggca atcaatgcgt 63420
ctgcaacggc atcatgttcc tacacgcctt gcacctgggt ggaacgagcg ccgtcctgca 63480
gaccgaggcg ctggacgcca ttatggaaga gggcgcgcgt ctggacgcgc ggctagagcg 63540
cgagttgcaa aagaagctgc ccgccggcgg gcggctgccg gtctaccgac tgggcgacga 63600
agtgccgcgc cgcctggagt cgcggttcgg ccggaccgtg cacgcgctct cgcggccctt 63660
caacggcacc accgagacgt gcgacctgga cggctacatg tgtccgggca tcttcgactt 63720
tctgcggtac gcgcacgcca aaccgcggcc cacctacgta ctcgtcaccg tcaactcgtt 63780
ggcgcgcgcc gtggtcttca ccgaggacca catgttggtc tttgatccgc acagctccgc 63840
ggaatgtcac aacgccgccg tgtatcactg cgagggtctc catcaggtgc tgatggtgct 63900
cacgggcttc ggcgtgcagc tgtcgcccgc tttctactat gaggcccttt ttctctacat 63960
gctggatgtg gcgaccgtgc cagaggctga gatcgccgcg cgtttggtct ccacctatcg 64020
cgaccgcgat atcgacctca ccggcgtcgt ccgggaaagc gcggacacgg cggcgacaac 64080
gaccaccgcc gcaccttcct tacctccgct gcccgacccc atcgtcgacc cgggctgccc 64140
tcctggcgtg gcgcccagca ttcccgtcta cgatccctcg tcctcaccca aaaaaacacc 64200
cgagaaacgc cgcaaggacc tcagcggtag caaacacgga ggcaaaaaga aacccccgtc 64260
cacgacgtcc aaaacactgg ccaccgcctc ctcctcctcc tcagcgatag cggcggcctc 64320
ttcttcgtcc gcggtaccac cgtcctacag ctgcggcgaa ggggccctgc cggccctggg 64380
ccgctaccaa cagctggtcg acgaggtaga gcaggagttg aaggctctga cgctgccgcc 64440
gttgcctgcc aacaccagcg cctggacgtt gcacgcggcg ggtaccgaaa gcggcgctaa 64500
cgcggcaacg gccacggcgc cgtccttcga cgaagctttc ctcaccgatc gtctccagca 64560
gctcatcatc catgccgtca atcaacgctc gtgtctgcgt cgcccctgcg gcccgcaatc 64620
ggcggcgcag caggcggtac gcgcctatct gggcctatcc aagaaactgg atgcctttct 64680
gctcaattgg ctgcaccacg gcctggatct gcggcgcatg cacgactacc tgagccacaa 64740
gaccaccaaa ggcacgtact cgacgctgga tcgcgcactg ctggagaaga tgcaagtcgt 64800
cttcgatccc tacggacgtc agcacggccc ggcgctcatc gcctgggtgg aggagatgct 64860
gcgctacgtg gaaagcaagc ccactaacga actgtctcaa cgactgcaac gtttcgtaac 64920
caagcgaccg atgcccgtta gcgacagctt cgtctgcctg cgacccgtag actttcagcg 64980
tctgacgcag atcatcgaac agcgacgtcg ggtgttgcaa cgtcaacgcg aggaatacca 65040
cggcgtttac gagcacttgg ccggcctcat caccagcatc gacattcacg acctagacgc 65100
cagcgatctg aaccgacgcg aaattctgaa agcgctgcag ccgttggacg acaacgccaa 65160
gcaggaactc tttcgcctgg gcaacgccaa aatgctagag ttgcagatgg acctggaccg 65220
tctgagcacg cagctgctga cgcgcgtgca caatcacatc ctcaacggct ttttgccggt 65280
agaggacctg aagcagatgg aacgcgtcgt cgagcaggta ctgagactct tttacgacct 65340
gcgcgacctg aaactgtgtg acggcagcta cgaagaggga ttcgtcgtca tacgagaaca 65400
actgagctac ctcatgacgg gcactgtgcg cgacaacgta ccgctactgc aagagatcct 65460
gcagctgcga cacgcgtacc agcaagccac gcagcaaaac gagggtcgcc tcacgcagat 65520
ccacgacctg cttcatgtca tcgagacgct ggtgcgcgac ccgggcagcc gcggctcggc 65580
gctgacactg gccttggtac aggagcagct agctcaactg gaagcgctag gcggcctgca 65640
gctacccgaa gtgcagcagc gcctacagaa cgcgcaactc gcgctaagcc gcctctacga 65700
agaggaagag gaaacgcagc gtttcctcga cggactctcg tacgacgatc cgcccaccga 65760
acagaccatc aagcgacacc cacaattacg cgagatgtta cgtcgcgacg aacagacgcg 65820
tctgcgactc atcaacgccg tactgagcat gttccacaca ttagtgatgc gactggcgcg 65880
cgacgagtcg ccgcgaccga cgttttttga cgccgtcagt ctgttgttgc agcaactgcc 65940
acccgactcg catgaacgtg aggatctgcg tgccgccaac gccacgtacg cgcagatggt 66000
caagaaactg gagcagatcg agaaagccgg taccggcgca tccgaaaaac gcttccaagc 66060
gttacgggaa ttggtttact ttttccgtaa ccatgaatat ttctttcaac atatggtcgg 66120
acgactgggc gtcggacctc aggtaacgga actctacgag cgatatcaac acgagatgga 66180
agaacagcac ctggaacggc tagaacgtga atggcaagaa gaggccggca agctcacggt 66240
aacttctgtg gaggacgtgc agcgtgtctt ggcccgggca ccgagccatc gtgtcatgca 66300
tcaaatgcaa caaacgttaa ccaccaagat gcaagacttt ttagacaagg agaaacgtaa 66360
acaggaagaa cagcaacggc agctactgga cggctaccaa aaaaaggtgc agcaggattt 66420
gcaacgcgtg gtggacgcca ttaagggcga gatgctctcc accatcccgc accaaccact 66480
ggaggccaca ctcgagctgc tcttgggcct agatcaacgc gcccaaccgt tactggacaa 66540
gttcaaccag gacttgctgt cggcgctaca gcagctgagc aaaaaactag acgggcgaat 66600
caacgagtgt ctgcacggcg tgctgacggg tgatgtagag cgacgctgtc acccgcaccg 66660
agaagcggct atgcaaaccc aagcctcgct aaaccacttg gaccaaattt tgggtccaca 66720
actcctgatc catgagacgc agcaggccct gcaacacgcc gtccatcaag cgcagttcat 66780
cgagaagtgt caacagggcg atccaactac agccatcacg ggcagcgagt tcgagggcga 66840
ctttgcacgc taccgcagca gtcaacagaa gatggaggga caattacaag agactagaca 66900
acagatgacc gaaactagcg agcggctgga tcgctcgctg cgccaggatc ccgggaacag 66960
ctccgtcacg cgtgtacccg aaaaaccctt caagggtcag gagctggcgg gtcgaatcac 67020
gccgccaccc gccgacttcc agcggcccgt cttcaaaacg ctgctagatc agcaggccga 67080
cgcggcccgg aaagcgctca gcgacgaggc cgatctgctg aatcagaaag tacagacgca 67140
gttgcgacaa cgcgacgagc agctgagcac ggcgcagaac ctgtggactg atctggtcac 67200
gcgccacaaa atgagcggcg gactggacgt gaccaccccc gacgccaagg cgctgatgga 67260
aaagccgctg gagacacttc gcgagctgtt gggcaaagcc acgcaacaac tgccgtacct 67320
gtcggcggag cgcacggtgc gctggatgct ggcctttctg gaggaagccc ttgcgcaaat 67380
caccgcggac cctacgcacc cgcatcacgg aagcaggacc cactaccgga acctacaaca 67440
gcaagccgtc gagagcgccg tgacgctagc gcatcaaatc gaacaaaacg cggcctgtga 67500
aaattttatt gcacagcatc aagagacgac tgccaacggc gcgtccacgc cgcgggtcga 67560
catggtccag gcggtggaag cggtctggca gcgactggaa cccggacgcg tagccggcgg 67620
cgccgcgcgt catcaaaaag tgcaggaact gttgcagcgc ttgggtcaga cgctaggcga 67680
cctagaactg caggaaacgt tggcgacgga atactttgcg ctgttacacg gaatccagac 67740
cttcagctac gggctggact ttcggtcgca gttggaaaag atccgcgatc tgcggacccg 67800
ttttgcggaa ctggccaagc gacgcggtac gcgtctctcc aacgagggag ccctgcccaa 67860
cccccggaaa ccgcaggcga cgacttcgct gggcgccttt acacgcgggt tgaacgcgct 67920
ggaacgacac gtccagctgg gtcaccagta tctgctcaac aagctcaacg gctcatcgct 67980
agtctatagg ctggaagaca ttcctagcgt gcttccgcca acgcacgaga ccgatcccgc 68040
gctgataatg cgcgaccgcc tgcgtcgcct atgcttcgcg cgtcaccacg acaccttcct 68100
tgaagtggta gacgtcttcg gcatgcgaca aatcgtcacg caagccggcg aacccattca 68160
cctggtcacc gattacggca acgtagcctt taagtacttg gcgctgcgag acgatggccg 68220
gcccctggca tggcggcgcc gctgtagcgg cggaggactc aagaacgtcg tcaccacacg 68280
ttataaagcc atcacggtag ccgtggccgt ctgtcagaca ttgcgcactt tctggccgca 68340
gatctcgcag tacgacctac gaccctacct cacgcagcat cagagccaca cgcaccccac 68400
ggagactcac acgttacata accttaagct cttttgttat ctggtgagca ccgcctggca 68460
ccagcgcatc gacacgcagc aggagctgac ggccgccgat cgcgtaggaa gcggcgaggg 68520
tggtgacgta ggggaacaaa gaccgggccg cggtaccgtg ctgcgcctga gtctgcaaga 68580
gttttgtgta ctcatagcag ctctgtaccc cgagtacatc tacaccgtcc tcaagtaccc 68640
ggtgcaaatg tcactaccct ccctcacagc tcacctacat caggatgtga tacacgcggt 68700
agtcaataac acacacaaaa tgccccccga ccacctcccc gaacaggtca aggccttctg 68760
tatcaccccc acccaatggc ccgccatgca gctcaataaa ctgttttggg aaaataaact 68820
ggtacagcaa ctgtgccagg taggcccgca aaaaagcaca ccacccctag gcaagctatg 68880
gctctacgcc atggccacgc tggtctttcc acaagacatg ctgcaatgtc tgtggctaga 68940
actgaaaccc cagtacgccg agacctacgc ctcggtgtcc gaattggtac agacgttgtt 69000
tcagattttc acgcaacaat gcgagatggt gaccgagggg tacacgcaac cgcagctccc 69060
caccggagag ccggtgcttc agatgatccg cgtgcgacac caggacacaa ccaccacaga 69120
cacaaacacg accacagagc caggactttt agatgttttt attcaaacag aaaccgccct 69180
agactacgcg ttgggctcct ggcttttcgg catacccgtg tgtctcggcg tgcacgtagc 69240
cgacctgctg aaaggccaac gtgtactagt agcgcgccac ctcgaataca cgtcgcgaga 69300
ccgcgacttc ctccgcatcc aacgctcccg ggacctcaat ctcagtcaac tgctccagga 69360
cacgtggacc gaaacgccgc tggagcactg ctggctacaa gcccaaatca gacggctacg 69420
cgattacctg cgtttcccca cccgcttaga gtttattccc ctagtcattt acaacgcaca 69480
ggaccacacc gtcgtacgcg tgctgcgacc gccctccacg ttcgaacagg accacagtcg 69540
gctggtgttg gacgaggcct tccccacctt cccgctgtat gaccaagatg ataactcatc 69600
cgcggacaac gtcgctgcgt ctggcgccgc tccaacaccg ccggtacctt tcaaccgcgt 69660
gccagtcaat attcagtttc tgcgtgaaaa cccgccaccc atcgcgcgag ttcagcagcc 69720
gccgcgccga catcgtcatc gagcggccgc ggccgcagac gacgacggac agatagatca 69780
cgtacaagac gatacatcaa ggacagccga ctctgcatta gtctctaccg cctttggcgg 69840
gtccgtcttt caagaaaacc gactgggaga aacaccacta tgccgagatg aacttgtggc 69900
cgtggcgccc ggcgccgcca gcaccagttt cgcctcgccg cctatcacgg tgctcacgca 69960
gaacgtcctc agtgctctag aaatactgcg gctagtgcga ttggacctgc gacaactggc 70020
gcaatctgtg caggacacta ttcaacacat gcggtttctc tatcttttgt aaccgacact 70080
gacagtagcg ggtaataaaa acaagaggat tgttatcgtt tttttatgat aaaaaaacaa 70140
cgtgtcattt tcacggtgat ttattcttgc tattattttt ccccatgggc tgtcagcgtc 70200
gggtgcgcga cactgctacc atgcgcaaca ggtccagttt aaaggcgcac ttgtcgttaa 70260
acaggctgga catgcgtgta tatttgctca gcatggtggc cagcaccggg tgggtggcct 70320
ctgagatctc ggtcggcaac tccaaaacga cgttgacgac gtgacggtgt ttttcgtccc 70380
gcttgttggc caccgtgggt cccggcgcgg tgttagacat ggggcaggcc gtggggggag 70440
gacgaggagg aagtcgctgc taaaccgcca cgcgcctgct gcacaatgtg gccgccgacg 70500
tggcaggcgg tctgtttaac cagcgcgcag ccccgacaca gcggggcgcc gtcttcgctt 70560
tccaaacagc tgtcgcggta ctcgcccgtc tgacagcgcg cgcacagcag gccgtgcccg 70620
tgcgaagtga ggcgcaggag acgcgggacc gtcacgccgc gtaccaccac agtggagtcg 70680
caggtgcgtg ccgcgcaggg cagaatgacg tcgaaagcca gccggtgatc gtacacggcg 70740
caagccgcgt tgaggcccag cacggctttc cagcccacgc gtacgcagcg ctgtccaaag 70800
agcgtctcgg agacgagctc gtagacgcgc tgccgcacca cccgctgact gccgcagagc 70860
gagcagtgta cgagctcggc gtgcgtgttg aagatgacgc tcttttcttg acggtcccga 70920
taatagaaca tcgagttgag cggaaaattt tgctggcagt gtagcttttc cttacccagg 70980
ttgaggcagt gtccgcactg ccgacagacc acggccacca gcgagcgcgc gtccagatgg 71040
cgctcgcact tgagtcgaca cagacaccag agcggcaggt cgatgacgct gccgatgagg 71100
ccgccgcgca gcgcggcgct gagtgcaaag aggacgatct tggtgggctc tacgtgacgc 71160
gcctgctgtc cggcgcccgc gtgtcctacc gccgcagctg ccgccgtcga gcctcctccg 71220
cgcgtctcgt cgtgcagacc cagtgcccgc aacggcacca ggtatcgcgg acacgtgtcg 71280
caaaacgtct gcaccgcttg tcgggccagt acgtagagcg ggtttccgca gggtaccttc 71340
ccagcgtgcc ggcgcaaggc tgcgatgagg ccccgcagct gcggcgaccg cggctgccgt 71400
tggtgacacc actggttacg gtggtatacg gccaaatcag cgcgggcgtc gaagcgcttg 71460
gcgcgtagta gtgctaggca cggcgagctg gtggggtgaa gcacgggcag ccgaaggtcc 71520
accccgaaaa ggaaacggtg aaggtcacct agcagcgagg cggtgacacc gtccaacaac 71580
gcgtgcagcc gctcgggcgg gtagagccgc agacggcgca gcaggtagtc ggtgtcgtag 71640
cgttcgaaac gcagaaaggc catcgtgcgg acggccacgg tgtgcagaca gtccatgctg 71700
tagacgtaag cgagaaacac aaagtagggc ttggtcataa ccatacgctg aaagagcgcc 71760
gtcaccgcct cccgctcggc ctgccgacac accagccatt cgcgcaggaa gcgttggtag 71820
agacggtcgc ccagctcccg attcagaaag cgcttatccg tcacgaagag atgaaggacg 71880
caagaacgtg gcacgtgatg caccagctgc tgctggagga ccgccgacgt ctgcgccgca 71940
aactgcgccg gtggctgcga cgtttctacc gccgcttcct ccggctgcag cgcaccgcgg 72000
ccgatcacca gctgcacatg gaaatggtcc tcgtgaacgc agaggggcgc gaagagacgg 72060
cgcagagcct ggtggaactc atcagtcgcg gtgtgcggag cgtgtcggag acgacgactg 72120
gccatgaccg cgccacagca gagccagcac cagcagaaga gccagcacca gcgggcccag 72180
agtcgcaaag cgcgcgggca gccacggccc agactgcggt cgcgatggcc cggagcgcgc 72240
tcgccaccac gatgacggtg cccaacgata accagtccgc tccaaggacg gcgcgcacgg 72300
cggagacggc ggatgacggt gatgggtcga cacccctcgc cgacgactca cgtgctcctc 72360
cagaggccga cgcgcggacc ctccgacgtc ctggcccgcc gctgccgccg ccgccttccc 72420
ttctcccgcc agagccagca actcctcctc ctcttcatca gcgtctccct cgcttgcgca 72480
tccgcatcgt cccatacagg cctcacaacg acacagccgc cacgaccccg ccgccatggg 72540
tggcggcggc ggccgaggcc cggcagcggc gccgccagcg gcgaccatgg tgggagagca 72600
actcggatga cgaggaggag gaggaggagg gggagatgcg gtccgagagg accgctttcc 72660
cgccgttcgc gtgagcgcgg ccgacatgcg ggcgcgccac agggacggac cgctgccgct 72720
gtgactgctt acggtgacgt ggttccggac cgccaacgac gtcgacgcgg ctttcttggc 72780
gtacagctcg cgcagcagat tctcgtactc gccctcgttt tcgggtccga aggcgatgag 72840
ctcgatgttg aagaccgacg ccgaattgga tttgcgcacc acgcacttcg tcagcactcc 72900
gtaggccgag ggcttgatct cctcgatgtc cttgagcgtg acgatgagcg actcgttcac 72960
cttaagcaca ttgaactcac ctacgtggcg cgccggcgag acgagcttga cgggcgctcg 73020
cacaaaacag cagagggaga cggcgcagcc agtgttttta aagataaaac aaggcacgtg 73080
gtctgtgcgg ctctcccagt agctgagcag atactcgaca caatagaccg tgtctgtctt 73140
gagcatggcg tcgcacaccg agtaattggg atttttacag ataaggccgg cgtcggtgac 73200
gcgcagctcg ctgggaccca acttgaggat acgccgcgtg gcctgcacca gatcctgatg 73260
gagaaccttg ttcatctcca tcgcaccgac gccaccgccg atttatttac ccggcgccgg 73320
ctcgtctttt ccctccagga ttccgttaat gtccatgagc ttgctgacga tcgccgttaa 73380
tagttgcgtc ttctcacgga ggatctctcc gtgactgcag gtcgcgcagt cgccgtgcac 73440
gtacttgagg aaggcggcgt acttctgacc cgcgttcacg aaatttaagc gcgcgtccag 73500
ggagggcagc aacagatcgt agacgcgcgg cagcatcggc tcgaactgta atagcagatc 73560
gtcgtcaaga tcgggtagcg cgtgcccgtc ttcaccgtcc tcgtcgtcac cacctccccc 73620
ctcgagccca ccgctcgtac cagccgcggg ctccgcgtcc tcgtcgatca ccagcggtcg 73680
cgtcggcacc ggagaatcca cgtcatcctg cacgtcgttt tcctcctctc cgtcgtcatc 73740
gtccagaaac ggcacccgct gcttagccca ggacattctt tctccgcgtc ctcaatcagc 73800
ggcgccgatc gccatgaatc cgagtaccca cgtgagcagt aacggcccaa cgactccccc 73860
ccacggggcc cacaccacgc ttcttccccc gaccagcccg gccccgtcca ccagctccgt 73920
cgccgccgct accttgtgca gtccgcaacg acaggccgtt tcgcgttaca gcggctggag 73980
caccgagtac acccagtggc actcggactt gacaactgag ctgctatggc acgcgcaccc 74040
gcgtcaagta cctatggacg aagcgctggc cgccgcggcg gccgcctcat accaggtgaa 74100
tcctcaacac cccgccaacc gttaccgtca ttacgaattc cagacgctca gcctcggcac 74160
ctcgggggta gacgaactgc tcaactgctg tgcggaagaa accacgtgcg gcggcacgca 74220
atccaccgta ctcaccaatg cgaccaacac caccaactgc ggcggagccg tcgccagcag 74280
tagcaacgca ggacctgccg gcgcttcggc cgcctgcgac ctagatgcgg aactggccgg 74340
cctcgaaacc tcggcggccg actttgaaca gctgcggcga ctgtgcgcgc cgctggccat 74400
cgacacgcgc tgtaacctat gcgccatcat cagcatctgc ctcaaacagg actgcgacca 74460
aagctggctc ctcgagtaca gcttactgtg cttcaagtgc agctacgcac cccgtgcggc 74520
gctcagcacg ctcatcatca tgtccgagtt tacgcatctg ctgcagcagc acttttccga 74580
cctgcgcatc gacgacctgt tccgacacca cgttctcacg gtcttcgatt tccacctgca 74640
ctttttcata aatcgttgct ttgaaaaaca agtgggcgac gcggttgata acgagaatgt 74700
caccctgaac catctggccg tggtgcgggc catggtcatg ggcgaagaca cggtgcctta 74760
caacaagcct cggcgccacc cgcaacagaa gcaaaaaaac aacccttatc acgtcgaagt 74820
gccgcaagaa ctgatcgaca actttctaga acacagctca cccagccgcg accgcttcgt 74880
gcagcttctt ttctatatgt gggccggcac cggcgtcatg agcaccacgc cactcacgga 74940
actcacgcac actaagttcg cgaggctaga cgcgttatcc acggcctcgg aaagagaaga 75000
cgcaaggatg acgatggaag aagaggagga tgaagaaggg gaagaaaaag gaggagacga 75060
tccgggccgt cacaacggca gtggcaccag cggggggttc agcgagagca cgctaaagaa 75120
gaacgtgggt cccatttacc tatgtcccgt acccgccttt tttaccaaaa accaaaccag 75180
taccgtgtgt ctgctgtgcg aactcatggc ctgctcctat tacgataacg tcgtcctgcg 75240
cgagctgtac cgccgcgtcg tctcgtactg tcagaacaat gtgaagatgg tggaccgcat 75300
tcaactggta ttggccgatc tgttgcgcga atgcacgtcg ccgctcggcg cggcgcacga 75360
ggacgtggcg cgctgtggac tcgaagcacc cacctcgccc ggaggcgact cggactatca 75420
cggcctgagc ggcgtcgacg gcgcactggc gcgacccgac ccggtatttt gccacgtcct 75480
gcgtcaggcg ggcgttacgg gcatctacaa gcactttttc tgcgacccgc agtgcgccgg 75540
caacatccgc gtcaccaacg aggccgtgct cttcggacgc ctgcaccccc accacgtcca 75600
ggaggtgaaa ctggccatct gccacgacaa ttactatata agtcgacttc cgcgacgtgt 75660
gtggctctgc atcacactct tcaaggcctt tcagattaca aaacgcacct acaaaggcaa 75720
agtgcacctg gcggacttta tgcgcgattt cacgcagctg ttggagagtt gcgacatcaa 75780
gctggtggac cccacgtacg tgatagacaa gtatgtctag cgtgagcggc gtgcgcacgc 75840
cgcgcgaacg acgctcggcc ttgcgctccc tgctccgcaa gcgccgccaa cgcgagctgg 75900
ccagcaaagt ggcgtcaacg gtgaacggcg ctacgtcggc caacaaccac ggcgaatcgc 75960
cgtcaccggc cgacgcgcgc ccgcgcctca cgctgcacga cctgcacgac atcttccgcg 76020
agcaccccga actggagctc aagtatctca acatgatgaa gatggccatt acgggcaaag 76080
agtccatctg cttacccttc aatttccact cgcatcggca gcacacctgc ctcgacatct 76140
cgccgtacgg caacgagcag gtctcgcgca tcgcctgcac ctcgtgcgag gacaaccgca 76200
tcctgcccac cgcctccgac gccatggtgg ccttcatcaa tcagacgtcc aacatcatga 76260
aaaatagaaa cttttattac gggttctgta agagcagcga gctactcaag ctctccacca 76320
accagccgcc catcttccaa atttattacc tgctgcacgc cgctaaccac gacatcgtgc 76380
cctttatgca cgccgaggac ggccggttgc acatgcacgt catcttcgaa aactccgacg 76440
tgcacatccc ctgcgactgc atcacgcaga tgctcacggc ggcgcgcgaa gactacagcg 76500
tcacgctcaa catcgtgcgc gaccacgtcg ttatcagcgt gctgtgtcac gccgtctcgg 76560
ccagcagcgt caagatcgac gtgactattt tgcaacgcaa gattgacgag atggacattc 76620
ccaacgacgt gagcgagtcc tttgagcgct acaaagagct cattcaggag ctgtgtcagt 76680
ccagcggcaa caacctatac gaggaggcca cgtcatccta cgcgatacgg tctcccctaa 76740
ccgcgtcgcc gttgcacgta gtttccacca acggctgcgg cccctcctcc tcctcgtccc 76800
agtccacgcc gcctcatctc cacccgccgt cgcaggcgac gcagccccac cactactctc 76860
accaccagtc tcagtctcag cagcatcatc accgtcccca gtcaccaccg ccgccgctgt 76920
ttctcaacag cattcgtgcg ccttgacact gtacggcaga aaagccggct ccaagtgcaa 76980
gcgccgcggc agcaccatgt gcaaaaactt gtccttgcgc gcggtttcgc cgccgggaaa 77040
gacgggcgac agcacgttgg ttacagcctt gagaacctgc tcaaagtact tgtcggtgtg 77100
aatgggcacg ccgtgctcgc gcacgtagct cggatcttcg gctacctcgt agttgcacac 77160
ggccgacggt ggtttccgcg ccctcttctt tgccggctct cctcctctcc tgttgctctc 77220
ctctaccccg ccgccgtcag cgtcgtcgtc cgtgccatca atcgcgtccg accgggaaac 77280
cacgccggcg gttacagaat caccgttgtc ggaggaaccc tgcggcgccg tccggacacc 77340
gggcgccgtc aggacgtaaa agacccgatc cccgaccgag ggtagctcct cagaacgggc 77400
cgccagtcgc ttaatgacgg caatgtgcgg caggttagat tgacggtaca acgagatgtc 77460
cttagaaagc accgacgaaa gcaccaggtc ctcgacacgc acacggtgca ggtacagatc 77520
gtcgcgggcc tgcaccaggc ggcgcaagat acgccagaaa ccgcgtggca cgccgtattt 77580
cttgacttca tcgagtgaga ggcgcgacag gcgcacggct gcttccgaga cctcgcgatc 77640
ctcaaagagc agcgagagga cgtcacgcgt gacgcccttg acgaactcgc aggccgtctt 77700
gcgcaccaga tccacgccct tcatgctcag acccgaggcg ccctccactt tgccgatgta 77760
acgtttcttg cagatcatca taagagagac gaagaccttt tcaaactcca gcttgacggg 77820
ctccacaaaa agacaggccg tcacgtagtg cgccaggctg ggcccacgcg ccaccagagc 77880
ctgcggcgtc aggccacgaa agcggacaaa cacgctgtcc gtgtccccgt agatgacccg 77940
cgcctccacc cgccgttcgt ccgagccccc tgacgatgtt tcgagcccct ccggtaacgt 78000
gctgctctcc tccgaatccc cctctcgcgt tctcactaca tagtcttcct gattaaaaaa 78060
attgtgcaaa aaacacggct ctgaaaaatt gtctttgatg aaccgcgccg tgcgctctag 78120
catgtcgcga ccgatgcgcg tgatgctggc ggcgatgggc agacacggca tcatgccgtt 78180
gaccacgccg gtaaaaccgt agaaagcgtt acacgttact ttgagtgcca tctgttcctt 78240
atcgagcagc atacggcgca cggggtcttg acactcgcgc atgcattcgc gcacggcacg 78300
gcgctgcgaa acccacttgt tgagcagttc cgaaagcacc gagacgcgca ccgaagcacg 78360
cacaaagcgg tgagtcacgc cgttctctag cgtgacgctg tatacgtcgg cggggtccac 78420
ggggtactcg ccacccggca ccagcagggt ggagtagcag aggttgtgag ccatgatgat 78480
ggaagggtag aggctggcaa agtcgaacac ggccacgggg tcgttgtaat aacccacctc 78540
gggctcaaac accgtggcac cctggtacga aaccgccgca gtaccgccgg cgccgtgact 78600
gtcgttggaa acgccgacgc tgccactact gccggagccg acgctgaaaa cgccgacgct 78660
gctactactg ttactgccag agccgggtaa aacgccgtcc tgactcgacg gcgcagattg 78720
caagggcggc gacatctgaa acatggccgc cacagaaccc gcgtcgccgg gcacggcagc 78780
ggtagagatg atagcggcgt taggtgacac ggcaacgcta ttcgtttcgg gcaccgtcgt 78840
acctttgctg tagtggttgg gcaggataaa atcgcggcag gcgcactcgt ccagcagcga 78900
ggtgtagata cggatctgct gtccgtcaaa gatgacacgc cgcaacggaa ttttagccag 78960
ccgcgcgatg gccccggcct cgtagtgaaa attaatggtg ttgaacagat cgcgcaccaa 79020
tacggcgtcc tgcagacagt aacggcctac ctgggcgcgg ccctcggcat tagccacgaa 79080
acaacgcggg atgtccttgt aggacaggtc atccttgcgt tgccgcaggt aaagctcggc 79140
catagtgttg agcttatagt tgggcgagtt agtcttggcc atgcatacgg ggtacatgtc 79200
gataaccacc gaacccgcaa tatacacctt ggtggcggcc gtgctggccg gattgttgtg 79260
agaagccgag ggaaaggcgg cggcgtactg ccgcttaaaa cccacggcgg ggctgtgtaa 79320
aaagaaacgg ccgccctgcg ccgtgggcaa cttgcagaag cgctgcgagt ccaccttata 79380
caggtactcg aggcgcgtga ggatgtactt caagtcaaaa gagttgatgt tgtaaccggt 79440
cacaaaggcc ggcgcgtacc gttgaaagaa aagcataaag cccagcagca gctcgtattc 79500
ggaagggaac tcgtagacgt ccacgtctgg gcccacctgc ccgcaggtgc cgatcgtaaa 79560
gagatgaaga cccgagtgcc caaagatcac accctccgaa gtgcagcccc gaccatcgtt 79620
cccgtttggg atcccctgat ccacggcggt gtttcccccc gtctcgtagc acacgcacga 79680
gatctgaatg acaatgtcat cggacttctc ggcgcaggga aaaccaccct cgccgctcat 79740
gcactcgata tcgaaggaca ggcatcgata gcgcggccac gagctgtcgt cgggcacggc 79800
caccaggtca gagacatcgc agtcgacctc gatatcacaa gtcgacgcgc gaccctgctg 79860
ccgccagtcg taacgattca cggagcacca gccgaacgtg gtgatccgcc gatcgatgac 79920
caaacgcgtc agcggatcca cacggacctc gtacacggga aaaccctgct ccagcagata 79980
ctcgccgatt tttctggcca tggtccagtt gctgatagac acacactgca aatcgggcac 80040
gggtcgcgtc ccgtacccgt agatggaggt cttggtggcc ggcgtgacag acacggcgta 80100
tggcgtccgc ggctcgggca ctagttcgcc cacgctggca atgacctcac gcagcctatc 80160
ggtgtcgctg tactcacagt aaaagtagct gcgctgcccg aaaacgttga cgcagatact 80220
gtagccgtgt tctgtggccc cgaagaaacg caacacgttc cccgaaggca ccagatgctg 80280
acgatagcgc ggcgacacgt tttcgggcga gtcgaagaag agcacggcgt ccgtctgatc 80340
gtaggtgtga aaacgaatag gtcccaccac gcgacccacc agggtctcgc gccaaggaca 80400
cggccaaacc atgtcatgac tcaacaaatg tttaatctct cgatagaaca tgagaggcag 80460
ccgtcccgtc ttatgcttga tcaaccccgt ctgaccgtcg aacatgacgc ctcgcggcac 80520
gatctgcaaa aactgtttct gtggcggccg cttgcccgag ccctgcgcgg agccgggctg 80580
cgaacgctga cgccggccac ccgcgaccgc accgccggtc acgccgccgc tcagatacgg 80640
gttgaaaaac atagcggacc gtgagaggct gacagcttac gaagcacaat cacaaagaaa 80700
atacacatgc agcacctaga tatccagttt gaccccgtat atcacaagtc tctgtgtcac 80760
tttttttgtc tgtttttttt ttcttctcct ggttcagacg ttctcttctt cgtcagagtc 80820
tttcaagtgt cggtagccgt ttttgcgatg tcgcagtcgg tctagcaggt tgggcttctg 80880
tcccttgtcc tgcgtgccag tctgtccgtc caaagaatct gtaccgttct gctgcgctcg 80940
ctgctctgcg tccagacgga ccagggccag aagcatctgg taagcctgct cgttggtgta 81000
aggcggagcc gccgtggatg catcagacga cggtggtccc gatcctttgc gaccagaatt 81060
ataaacactt tcctcgtagg aaggcggagc ctgtaacgac gtgtctttgg tgttgcccga 81120
cgtcacggtg gtcccgttgg cggacaccag atagggaaag aggttctgca gcggctgcat 81180
gcagagacgc cgctgtcgag tatagatcaa ataagtgata atgactacgg ctatggccac 81240
gaggatgatg gtgaaggctc cgaaggggtt tttgaggaag gtggcaacgc cttcgaccac 81300
ggaggccacc gcgccaccca cggccccaat ggctacgcca acggcctttc ccgcggcgcc 81360
caggccgctc atgaggtcgt ccagaccctt gaggtagggc ggtagcgggt cgactacctt 81420
gtcctccacg tactttaccc gctgcttgta cgagttgaat tcgcgcatga tctcttcgag 81480
gtcaaaaacg ttgctggaac gcagctcttt ctgcgagtaa agttccagta ccctgaagtc 81540
ggtattttcc agcgggtcga tatccagggc gatcatgctg tcgacggtgg agatactgct 81600
gaggtcaatc atgcgtttga agaggtagtc cacgtactcg taggccgagt tcccggcgat 81660
gaagatcttg agactgggaa gctgacattc ctcagtgcgg tggttgccca acaggatttc 81720
gttgtcctcg cccagttgac cgtactgcac gtacgagctg ttggcgaaat taaagatgac 81780
cacgggtcgt gagtagcagc gtcctggcga atccttcacg ttcatatcac gcagcacctt 81840
gacgctggtt tggttgatgg tcacgcagct ggccaggccc aagacatcac ccatgaaacg 81900
cgcggcaatc ggtttgttgt agatggccga gagaatggct gacgggttga tcttgctgag 81960
ttccttgaag acctctaggc tgcgccgttg atccacacac caggcttctg cgatttgcgc 82020
cagcgcccgg ttgatgtaac cgcgcaatgt gtcataggtg aactgcagct gggcgtagac 82080
cagattgtgc accgattcca tgctggataa atgagttgca ttgttgccat ctgcacttct 82140
tttggttcta ctatgagtaa gattcagact ggagcggttg gccaaacgtt cgagttccac 82200
cagagatttt tgcttgatac cttgccagaa caccaccaaa ccaccagtgg tttcaaacac 82260
ggacacgttt ccatattttt catatgtttg attgtatgaa gtattgaaaa tctgctgtaa 82320
cttatttatg gcctcatcac gtacgcagtc cagcgcagag tcggacatgt tcacctcttg 82380
cttcttagat aagaaagtgg cggtcatttt ggcagaagaa aagtgatacg agtcctcggc 82440
ttcggaacga atggtgcgtt ccgaggcttc ccagaaagtg agttgacaag taacattctt 82500
ctcgtcctgt atatcccagg agatcactga gtccgcacgt tcaagaaaag ccaccaacct 82560
gtgggtctct aacgcagaat tcggtcttcc aaagtcggag acgatagtgt agttcggaaa 82620
aatgaaaaac ttgtcggcgt tttctccaaa atagctggca ttgcgattag ttccgttgta 82680
gaaaggagaa atgtcaacca catcacccgt ggaagttgcg aaaaaatgat agggatactt 82740
ggagcgcgca gtagtgatgg tcaccataca attcagatta caggtctcac gatagagcca 82800
ggtgctgccg cggctgtgcc attgatcctt gaccgtcacg taacgggtac tgtgggtgtt 82860
ggaataatcg tcgggcatta attgcatggt tttgttttca tagctgtccc tatgataagc 82920
cacgaaaacc gtgcctgcta taacgcggct gtaggaactg tagcactgac tgtggctgtt 82980
gatatgatga atctcccaca taggaggcgc cacgtattcc gtgttgctgc ccagcagata 83040
agtggtgtgg atgtaagcgt agctacgacg aaacgtcaaa accttctggt agactcgtac 83100
cttaaaggtg tgcgcgacga tgttgcgttt gtagaccacc atgatgccct cgtccaggtc 83160
ttcattgata ggcttcatcg aggtgcagac gatattacgt tcaaagcgaa taagatccgt 83220
accctgtgcc atagaacaca cgcgataggg gtacttggtg gtattgaccc ccaccacatc 83280
tccgtacttg agggtagtgt tgtagatggt ctcgttaaca ccatggctga ccgtttggga 83340
agaagttacg cgttgagaga ctgaaccgga tcgagagtga gcagcagacg tcgtatgaga 83400
ggaatggtga ctgtgagtag cagaagttcc acgagaagta gaagatgagg aaaccgcagc 83460
acccagacag acgatacaca agttaacgca gactaccagg caccagatcc tggattccat 83520
gttcgtcgcg ggccaaatcc agcagcgatg aggcgcgtcg tggtctcttg cgtgttgcgc 83580
ggaccctccg ggaaacgccc gcggtcgagg aggaggggta cggacttggc agccaaggtc 83640
ggtccggctc cctgaaggca cccgagacgg ccgcggcggc cgttagggtg gagggcttgg 83700
ccacgggagc tgttggcacg tcgccactct catccggtct ggacagatgc ctgtagagga 83760
ggagatatag atctttggac ttataaagac ttccttcgtg acgaagcagc agcggccact 83820
ctttgttata cgtgagaatc acatctctgt ccgggtgcag ttcgtcgcgc aggcacgcga 83880
tcgagagttg tttcccgaaa gtttcattat atagtgcgac ggagagcacg agctcccgca 83940
cgtgcatcca catctccttc tgcagcacgt ttaggtcctg acagtccgaa aaattgaaaa 84000
aacccatgta cttcaccacc atccactcac tgggatacac ggtaccttcc gcgcatttga 84060
ccaaatcgtc cttgacgtgg ggtagtacgc ccgcgttgtc gcaggcatag gccatgtcca 84120
cattgtgaga gaggggatag cgatcggtac agtgtgtgaa gaggggcccg ttacacaact 84180
cgtagatctg ctgacccagt agcgggaggg attccacagg cagactcttg tggatcaggt 84240
tattgaccac atacaggtgc tcatcgtacg tgaactgatc ccccacgtcc accacgtctt 84300
ggtcctggtg gtattggctg cggtatagaa acccattcat gagcttagag ataaagtcca 84360
gacacaaggg ccccactagg ttgacatcga tgagtttgct agtcagacgc tcctgcgttt 84420
tgatgcaacg gatcaccttg ccatagccca cctctgagac cttctgcagg taggcgcgtt 84480
tgcgcacgtt cacctcgcgg gtgacgttgt ggatgcggga acgcgcgtcc accaagtcga 84540
gagcctcgtg ttcgtcgcag ttgcgcaccc gtaagccgtt ctcgccgccg tcgccgtcct 84600
gcccattcgc ccctccccct accgctttct tgcctcctcc acgggcccgg ccgccgccac 84660
cgttattcct ctgactgtga gtactgctgt tgctgctgtt gctggccgtc atcaaagtcg 84720
tacccgtccc cgacatcgcc tcccgtccac gcaggtgaat agcctcgccc tcggggccgt 84780
cgccccccgt gccatcgggc agcggacgtc gaatctcctc gagaatatgc ttgattttgg 84840
tgtacatctc gttgctttcg tggagcttgt tgaacaccgg gttgtcctcg aaagcttgaa 84900
tgctgaggga tgtgatgagg tcgatgatcc tgttgggggc ggcaaagacc gaccccacga 84960
acatgcgctc ctccccgtcc aacgcctttt ccccgagcac gaagatgtcc tccacgtcct 85020
ccccgtacag atggcgactg atgccgttca tgagcgcccg gcacagctgg tgatacacat 85080
ttagctgctg gatggtgatg cccacccgct tgacgataac ctccgaggta cgggaccagt 85140
aggtaaaatc cgacaaggaa tatattcgtt ccggtatatc cgtaaacagg ttgtactccc 85200
tcagcgcctc ctccgcctcc tggatgtagc tgtggtaggc cgatgaagaa gagaataggc 85260
ttttgagggc cgaaaggact ccagccaagt gggggatgcg cgttgtcagg tccagcaggt 85320
cctgctccac cgtctggata ttcacatcgg actggcttga cggacggtgg accgctatat 85380
ggttgcacag caagccctgc agccgcttgt tcagcgagcg gccctgattc gggatgatgg 85440
tcagctcctc gtagcattgg gcgcatgtcg tcccttcgac gtacacttcc tgacgcgcca 85500
ccggcgagat gccgcatagg cgacggagga gctccagcag ctgcgcgcag acctccaggc 85560
cggcctccgg cgccaggatc ccgtacacgt agttcatttt gcacaggaag cgctcgatgt 85620
cgttgagtgt ggccagactg acgctgaaac ggacgttgtc cgtaaactgg agctccacgg 85680
tgtgatggcg atcgcagcga tccaaacgga ggacggtacg gtagaaggcc gcccggtccg 85740
gctggcgcga gtaggccatc agcgcccgat ccagcaaagc cgtatcctcg tgcagcgcct 85800
tcagcagcat ctccagatag agcgtcagca gcgaactctg cgtacgattc tgcgccacca 85860
cctccgggta gatcttccgg tacagataca ctatagccgc cgcgtttctc ttgaacggcg 85920
tggactccgc cagtaacacg ttcggatcgc agtactttag acactccagc tccatggcgt 85980
attcgttgca tttcgaacac actacgcata gtttctgtaa caaattcatc tccatgactc 86040
gactcgctca cgtacgagac gctgtcgtcc ggtctggcgc cggccagaga catggagtcg 86100
gtgcacaaat aactcgcggg ccgctcgcta tgccgactga cgttgacgtt aatatataac 86160
gacgtcgtcg acgacgcggg ttctgctccc gaagctgttg ccgccgcttg cggcgcaacc 86220
tcctccacca ccgccgccgc cggctcctcc gcctcgggcg acgggggctc ggagatgacc 86280
ggctgtgtct gacactcctc cccttcctca ggcggcccgg gcgccgacgc gaatgtcgga 86340
gtttgccagc gcggcggcgg tctctgtctc tggtgccgcg gcgctaacct tcggggctgt 86400
tgctgctgtt gatgatgcga cgccgtctgt cgccgctgtt gcggcggtag ctgatacggt 86460
gtcgcctggt gctgctgtgt cggtggctgc tgttgctgct gttgttgcgg tctgaaaagc 86520
ggccacgggg gctgcgactg ttgctgctgt tgttgcgatg ctcgtggctg cggcggccgt 86580
tgtcgcggcg tttgctggcg gttacaaccg gctgcgtttg gccggcaata acccgctgcc 86640
cccgccgccc ccgctgctcc cgccgacgcc gccagcctcg tcttcgccgg cgttcacgag 86700
aaagcagcca cctcccgtct cgccgggcac gccgaagcaa atggagttgc ccgcgacgga 86760
ctcgccgaga agaagaccgc cacccccgac gccggacgcc gcgccgacgc cactgggcgc 86820
gaagagcgcc gacaggtcgt gcacctcccc cccggcggcg tccgttaatc gctgggcgtc 86880
ggcgtccagc acgcgtcgca agttctccag cgaaaagtcc tccacgccct gctcctgcaa 86940
cgcggcaaac ttgtccatca gcgacgcggc cagcgcctcg cagccatcca cgaagaagag 87000
cacatcgtcg gacgcgggga tctcctcgcg cacgctcaga atctcgtaca cggccatcac 87060
ttcggggtcg caatccaagt tctcggcgtc cagcgccagc atgacgcggt tttttataag 87120
atccgcgtca aaaagcacgt tctcgcggcg cgagcgtttg atgagcacgt cggccagacg 87180
cgtagccaag aggtagcgct ggcgcatgaa acgataatct tggccgctca tagagctcac 87240
gttaaggctg cgttccacac cgttgcccga aaagtagccg atctgcccaa actgatagat 87300
ctccttgctg ttgttgatac ccgcatactt ttccacgctc acgggcacgg tcaccaagga 87360
acgatgctca aaaacgctcc gtaccaacga ttcacgcgcc acagtggcgg ccatgggcgc 87420
cggcacgcct gcggtcttca agcccttgac atgcaacgca aattcggcgg gcgacgagaa 87480
acgcggacta gcacctaaca cgtgaggaaa ctgcgcgtgg ttctgcgtcg ttaagcgcgt 87540
cgtcaacccg tgcagcgagc caatgtagtc tttgaagccg tagtagcaga ggaatttgtt 87600
atggaaacgg ctttccacgt aactcagcac acagtctggc gccacatcca gcagatcgtg 87660
ctcctgatag tcagccgtca cagccaccag aaatttgacg aaagcattga actcgcccat 87720
gtcacctatg ggcacattct tgggcaacgc gttggaacag accttctgcc aaaactgtaa 87780
gcaggggaga ccacattcag gaaagagtcg ctcgtgatgt cgatacagca gaaatcccaa 87840
gcagccctta gccggattac gacgcggaac gtgatcgcgg cgaaaaaaca cgctacccgc 87900
gttgcccttg cccgcgcggt agatgggtcg gtttttcact cgcaccatga tcaacgtggg 87960
taccgacagc cgcgagagct tgatctccat gggcaccacg gcgtacgtgc cctgcgcgta 88020
cagcctaaag tccagcaggc ggtcgtgatc cgaattcttg gacgacttga tctgcttggt 88080
gaagagaaag cccttgcgcg acgacgtggt ggagaacgcg ccgtgaatgg attgaaaatg 88140
ctgcgtcatc catttggata ccaagttggt ggtcaacgga ttgtccacaa tgtatgaggt 88200
agcggtaata agcgccacgt tctggatcac gtaaaagacg gatctgaaat aggcgtaggc 88260
cagcagcggc tggaaggcca cggcgtaggg attcagatcc aggttgaagg cctgcgtggc 88320
gcccgccacc tcgtcgcggc tgctcttgag gcgcacctcc gaaacgaaac ccagggcctc 88380
gtcgtccaca aacttgttga gcgccgaaaa gacggccaca aagtcgcttt tgccgtgcgc 88440
gctaaaggta tcctcgcccg tcacggggtc gatgagccgc atcttgcggc agtaatccaa 88500
gatgcgattg agccgatagg tacggtccac gctagcgccc agcatgcgac cgccgcgccc 88560
catcattccc ccggaatccc cgccaccccc accaccacga ccgccgccca gaccgtcgct 88620
cgggcccccg ctcacgtccc gtccaccacc cccgccagca ccgccgcccg gaaccccgtc 88680
gtcacctttg ccgtccaaac ccccgtcctt ggcgtcgacg ttgtaacgcc gaccgaagct 88740
gcccaaaata tccacgtcgt tgagaaaacg cgactgcacg gtgatcacgc agggctcctt 88800
cttgggctgc ttgggcacca cgggcaagcg ggtgcgcacc cgcacgaagg ccgtctgata 88860
acacgtgtgg caacaagtac ccccacaggc ctcgcacagc cccgcggcgc agcccaccag 88920
gtgattcgtg agcgtcgacg aacccgacaa gcccgtgtta tacaccgaga cacgatttag 88980
ataccagacg aagcccgaaa ctagctgcgg acacgtgcca cacaccaacg ccaaatgctg 89040
cggcccatag cgttcgtcct tgagcggcgc gccttgaaat ttgagcacct tgcgcgcgtc 89100
gttgtagacg tcttcgcagg ccgccgacaa cccgttggtg aactgaatag ccttgagcaa 89160
cgtctcctga ctggccgtac cgccggcgct gggatgccgc gccgacgact ggagatacac 89220
cagcctgtgc tggtagagca ccgaattagc gctgaagacc aaggcggcca cgtgcgtcga 89280
gagatgcaac ttgagctcgg tcagcgcgcg gatcagatcg cggtgatcgg ttgcgttggt 89340
cactaaaggc cactcggaaa agagcataga ctcggcaggt tggtaggccg aatcgaaaaa 89400
taccgaggca aaactgaagg ccaactcgca aaccaccgcg tcactcagca tcagatgatc 89460
cttttccaga ctgctgagtc gctggctcat gtaccccaag tagcgcttat gtggcgccag 89520
cttcaccgac tgctgactgt cgtgcacaaa ctgccgcaac gccgcctcga tcagcacacg 89580
cggctccgag aagcgcagcg attgacacca tgacgtgtac acgtagtaga aaagcgtctc 89640
gcttacggcc ggcacgtaga gccctcgcgc ctccacaaaa gcgctgcgcg catccagcga 89700
gacctcgtcg gcttcggcgt caagctgcag cgaattaaag agcgtaggcg ggtacaacgg 89760
cacgcgcacc gcctcgccgc cgtacagtcg caccgtggtc gcctcctcca cgcatggaat 89820
cagctgaccg gcaaagagaa actccttcaa gccgttgccc accaccacgt gcacagtcgt 89880
ctcggacgcc tgacagccca ccgccgcgca caacgccgac agatcggtag gcacgcgatc 89940
cgcctcgggc gtgtaggcct ccaacgcgta cttctggcgg gcgtcctcgc acaaccgatg 90000
cacgtctccg tgatcctcgg taaaagccac gatgccttgc gtatgatgaa agtagagcgc 90060
aaaaggacag aaggacgtga ctttcgtgag caccccgccg tcgtaacaaa gcacaggcgt 90120
gcgcacagag acgccgaaat ccgcctccac cgtcagcccc gccaacagag gagcgatcac 90180
cacgctcgag gaacggtcgc atagcgagag agtggccaga atctcctgcg tttctgcgtt 90240
caacctgctg aagtagagaa aagccgcggg ccccaccggc gctagcgcgg ttagttcctc 90300
gtggctcatg gtggatgaac ggaagacaat ggctacgccg ccactgagtg aattttatac 90360
caaggaaaag ttcagcacgt catgtttgac gcacgacgtc tgatacacca ccgtggccac 90420
cactgcggtc tggctgcggt tgcggaccac caaaggcgac aaccgcaacg atcccagcaa 90480
ttcgtaagaa aagctaaccg ttacggtcgg gcagcctctc gcagccagac cgctagccga 90540
cgcacccgcc cgcgaaaata gcgtgatgtt cgggacggct tcgcgtcacc gcaaactaac 90600
gtcggtagtc gcgcacgtcg tttatcctca gcacaccgtc cgatcacaac ccgttttccc 90660
actcagtcgc acaagcagca cataaaaacc ccacacagtg cacgtgaaaa caacgtccct 90720
agaaaacggt gttttctgtc ctaccgtcac cgggccacac aggcaaatcc cgagcccgat 90780
ccccgaaaac accgtacggt gtttgtggcc tccaaaatca catcagataa caaaccgtga 90840
aaagtcacgt ttcacgaaca cggtgttttt aaatcacaaa gaaccacctg acggtttaca 90900
agcagaaaca ccgcaccacg gtggtacaag cgcggtggat ctggtctcgc aacctcaatc 90960
gccgctatca ccaccgactt tcgctgcgct ccgccgacaa aacgccgtat aagctacaca 91020
ccccaaaaac ccgcacgcct atgggcgcca aacgtgtgta ttatctcaac gtcacgacac 91080
gacacaaaca gcgtaacgtg gtttcccgaa cacgtacgcg gcacagaccc ccgacacgta 91140
ctcgaagacc ttacagttta cgagtcaata aaacaggaaa agatccgaac tttaaaattg 91200
tgtattttta ttttcccatc cccctctttt taccaaaaaa cacatttttc gtcttgtaaa 91260
aagtaacttt cgcccattgc catgaaacac cgtgatgggg aacggtgttg tgtgtcgact 91320
gacgtcacta cggcgatcag tatcgacgtc gtgtatacat aacggtgccc ggtgttttta 91380
ttcggggcgt tgtcgcgtct tgatgtaatg taacctgaaa ccgccgtgtc caagaatgcg 91440
gaagccagcg tgtaatcata acggggtttt gggtacaatc tgacgacatc tggcggcgag 91500
cgtacaccat cgaatgtggc gatcgccggc tctacgtcac aatgacgcaa aaacacactg 91560
taaaacacgc gtagacagct ttcctggtca acgagcgcca tctggtgtcg gcataagaac 91620
aggcatcaac cccgtggccg gcgaggcggt gagcactttt gctggtcacg tgaccatcag 91680
cgcaggaagc gaggcccgta gaaccgccca agaggcggtg ccagatgcca acgtcataat 91740
cacaaggtga tttgttacgt cacgcgcgcg cacgcacgcg cgcgcggtag aatacagcga 91800
tccctagtga agccacaccc attacgtgta gccatatccg cttacgtata cagccacacc 91860
cctaggtacg ccaccttatc taccaatcat agaaacggat atacaatgac ccctccctag 91920
actccacccc ttgtacggaa atttcagata ggtggaaccc gttagggttc caccgtcctc 91980
ggtgtacgta caggcttctc cgtctaccgg aaatatacac ctgctgacgt agacgctact 92040
cccggatacg cgtcataagc tactggaccc taggggggag tgtctacagg gctacgtgca 92100
cgccccctta cttagggtat ccgccccctt cctctgtttt ggcctagtaa acttaacgcc 92160
gccgcttctc acgtgacccc tggcaagcct acgtcacact cgcgtgacca cacccactcc 92220
ggatatacgt catcctgtgg aattccggac atacggtgac gtagcgagcg tagcgagcta 92280
cgtcacgtat gcgtacgtca cctccggcgg aaatcatctc tgatgacgta gcgagcgaag 92340
cgagctacgt catcagtccg ttttacgtat accgggtgct aggcgacgcc ccgtaggggc 92400
ggagcctagc ttccacccct aggatgcata ccctatatag cataattctt ctaacgaaac 92460
gttctacgaa aacggactgg cggaacggga accaccgtaa cccccccccc tcaccccccc 92520
ccttctcctc cggaaccggg gggggcaaat ttttaccaaa tttgggcaac catgttttcc 92580
aatgggacgg cgtttccgtg cgcatgcgca gtcggggcga atttttggtt gtcagggcgt 92640
tgccacgcgg attatgggat gggggctcga gtgcgcatgc gccggggatg ccgcatggaa 92700
agcctatata taaggagggg tgaaccaggg gccccggtgc gcatgcgcgg accaggcccc 92760
gcgggagggt cgccctgcgc atgcgccggt aaaattccac tgggtgtgtg tcgtgcgcat 92820
gcgccagtat ttttccactg gaggcggtca gtgcgcatgc gtcggtaaaa ttccactgga 92880
tgtgcaccgt gcgcatgcgc cggtattttt ccactgggcg gccgcaccta gggagcgcga 92940
gccccgtgcc gggcatgggc cgcggcggtg gaaaattacc gctccgccca cctaggcggg 93000
gcatcttaaa acctataaaa cccggcgtac ccgccgcccc ccggcgcagt ccgcggcagg 93060
gttccggccg tgctgcggtc cgcacgctgc gcccgctccc gcctgcctcc cgccctaccc 93120
cccaccctcc ccggccgagg cccggcgccg gtccgtccgc gggcccgtcc caccgccctg 93180
gagcaccatc cggggccgtg ggccgggcac cgggcgcggc ccgctccgga cctcggccgg 93240
gggtccctcc cctccccccg ctcgaccccc ccatccgacg gcccggccgg gctgggaccc 93300
ccgcaccggg gtcccggttc ccgtccgcgg cccgggggga cccgagcggg ggcttcccac 93360
ccccaccccg ctcctccccg ggctccggcc cgggatccct cgctgctccc ggcgacctcc 93420
gccggcttcc cggtccaccc gccgcggaac ggacgggacc cggggtccgc gcccttcccc 93480
tccccccacg gggggctggg tcacggaccc cggttcctag gctcgttccg cggtgggcga 93540
ccggggatcc cccacccagc tccccttccc ggcccgcccc gctggctttt gggcccctcc 93600
gggctttttt tccggctggg ggtcgcggcg ctcggccgac gacgacggta ggtgggccgg 93660
gtggacggtg gtggggacgg gcgacgcccc ggctcgacgg cagtcggtcc cggaaggttg 93720
ggggctgggg gcccggtcag gagctccggg agcggggtcg accgcgacgg cttccgggtc 93780
tcgcggcggc tccctctcgg cggctccggt tgggctcccc tcccccctct cgagggtccg 93840
gccgccagtc gtgaccgggg gtccctcggc ctagccgccg gctctcggtc cgccttatcc 93900
tgggcgttgg ccggtcccgt gacgctcccc tcccccgctg ctccccaaaa aactccgccc 93960
gaaccgtcgc ggcttgctgg ccctgggcgt ggtcccccac tcccctcccc ccatcggccg 94020
cccagccggg gtcggcgcct cggaccccac caggctgtgg cgtgtgtgct ggccgatgcg 94080
gcggcgaggt tgggtgtggc cggaagcgct cggggtcgac ggtgggccgt catgacacct 94140
caattgccgt cagtacgccc ctccacaatc accgtcccta cacgatgggc ccggcaggtc 94200
acccaacgtt ggttcaggcc cagtcgggtt tttccccggc acaaacgcac gtccccgtgg 94260
gctccacgcg ttttccaccc tttcctggag gggtccggaa caccgtgaat ccgcggggag 94320
ggtctcggca cgggccgagg agaccacgac cgtcccaccc ggcgtgtcga cccgtccgag 94380
acccgggaag ggaacaggcc ccaccctttt ttttcccttc tccgatttgc cgtggaaaac 94440
ccgtgaaccg atacgggtac agacggccga aaaaattcga gacgacaata cgacggcagg 94500
gcgtgatttt cttccccatc cgacaaaacc gtgtccctca aaattcccca cctttctctg 94560
ttcaaatggc cccgaaactg taaaacaccg ttcgaccgca ccccaaccgg cgccatcttg 94620
gtgaccttct cgacggttct ctcgctcgtc atgccgttct gagctccgac atggcggacg 94680
agagaaaatg gcgtcgagag cataggagcg ttttcgctcc aggcgggtaa aagaatagca 94740
cgataacttt tctgtgcttt ttttgagacg ttttagaaga gctttttttc tgctcagagc 94800
gaaaaaatga tagccctgaa aatctcgacg agtctggccg agcggcgcca tcttggagga 94860
ggggcgagtc gcgggcaccg cctcggtacc ccctggccga ggcgagtccg cggtcgccgc 94920
ctgttccgtg atgctaccta gagggcgccg tcgaggcgac tcttcctgtt ttcgccctga 94980
gggctaacgg tcgctgacgt caaaccatct cgtgctcgct gagtcacatc cggttgttga 95040
caagcgatgg aggaccgcac ccaaagtgcg ccctctagtc atcgcgcctg acccctttta 95100
taaactgctc gaagaaaaga acaccttatg tgaaaaaata cagaatgatg acaagttcat 95160
ccaacacaac cgctcaacaa cgccatatct atcagtgtcc aaaaactatc ttctatcctt 95220
tgaaactata aatgctgcct atatacatat ttagtatcca agactcttac cacgtagacg 95280
aaaagaagtg atacaatgat cttgacgtgt atcgtctata tcgtgctaga tatattcaga 95340
taagacgcgc aaaccataga tttctcatca gtatcatgaa agacctatag ctctatatac 95400
gaacctagtc attttaggac agccgccgga gaagccgacg agggatcggg cgggtgcagc 95460
cagaacctca cgcccgatcc cgcctccggt aggcgatttg catctgtttg gtaaaaagct 95520
cataagtctg tatgtgacct atatatatat tatacgctat gtacaccgaa ctgtcgctgt 95580
tgtataagaa gaaaaaactc tccatattta tatcgtctga atttttgctt gatagacacg 95640
tgtttggaac tctgtccccc cacgttttca ctgtgtataa caaaaatatg tgtttctcaa 95700
aagatcttga ggtgtttgaa aacgggggaa acccgcgttt gggtgcgcta agccccggac 95760
tgggacgtag ccggcgtccg gcacctatat ttttctattt tttttacaaa atatatgatg 95820
aaccaagaat aaaactctag ctctcgtcta tttttaatat gctctactta gaaccttttt 95880
aatgacagaa tgaactccat gttatacgct ctttatatag tttctctgca ctaaccttta 95940
aaatcgtatc cttccctgtt gtacaaatca tcttttgata cacaatgatg acctgatatc 96000
cctccatata tatgatcgga tattattccg ttagacttgt cctccttttt tttcctcatc 96060
tcctgtatct ggagatatat gttgaccacc accgccatga ccaccaaaaa gctagccgtc 96120
acgactagaa atgtgtagga ttcggacttt ccgttcgaga agaaagagac cgcgtctctg 96180
gacgctcttt ttgtcggtct gaatcgaccc gggatacgta agagagcggc cctacatcgg 96240
ggggcgctcg agaccgacga cgttccatct gaccagaaaa aaaaaaggca cccctcggta 96300
gcgacctctc accatcgttt gcccgtccgc ccgtccttcg tagccatcat catcatctca 96360
ggctctatcg gtaccatcgt tgtcatctga aaaaaaaaaa ctgcctcacc cacctgcgta 96420
aaaacaccat ctttccggag gtgcggtaag acgggcaaat acggtcgtgc cgaggcaaaa 96480
aaacgcacca tcgacaccac accctcatga gcaccacctg tcggtgttgg tcgtcctcca 96540
tcgttctcta cgaacatctc gacgcccggg tgacggacga cggcaagacg tcccggagaa 96600
gacggtgttc tctcgggcgg tacgctctct ggatctataa tatctatagt agctaaacga 96660
gactgtgagt acgacgaacc acatcatctt ttttttatgt tgctccttta gaaaatgact 96720
tatgtcgacg acactcggca tcagccatct cgtgaaacac gctcgctttt cgtctctcca 96780
aggaacactg ggtccgctga aagggaccgt gtaccgacca aagcaaaaaa cacacacgta 96840
gtaacatgat caaccacgtc tgaatgacac gaaaacacaa tcgtataacg ctctattcat 96900
ggaacgaact tggaataaaa aaaccatcgc aggccagagg ctaagccgaa accgtccggg 96960
gaagcgggcg cgagttttcc gacttagtct ctggtgctcg ttgagcctct ttttttttcc 97020
tgattctctg aagaatcacc gtcacagccc tatgacgcga aatcaattgc tagaacataa 97080
acgttctcaa caggtatgaa atgaacaaac tagatgatgc tataacctta tattgtgtgt 97140
atatagatag gtgtgaaatt tgtaggataa aaagtgtcgt tgtatgatgc acaacgatcg 97200
tgaaactgga gactgtagct ctctaccgaa tgcaaataca caaatgacat cgattcccgt 97260
ccccacataa agaaatgtgc tttactgtga aagaatgaag aagattcttg ttcctcgtac 97320
gacggggccc tcgctcgtcg tacctcttcc cccctccggg agaggggacg tcggggccct 97380
ccgtcgcacc gggccgaagc cagtgaaatg tttactacac tgtcatcaga atatatgatg 97440
tatattattt cctccaaact cctcaccata gccaccaatt cgcatcactt aagaaagtag 97500
tagcaaccgc ggcggcggcg accggccggt cgtcgtctcc tcgtcctcaa atgttgtaca 97560
tgtgcagaaa aatgtgtaaa tacgtgttat ttatcccatg cgtcttgtac atagatatat 97620
gtttttatat acgctattta tactttatat atccttttgc ataaccatag acagtcaagg 97680
attttaatga tttgctcatc cgcctttgag ccatcgctta ggagttagtt cctctatgtt 97740
ctcggcccac cttttcgact acagtagcaa acccttgtac taccaccccg ataaaaacca 97800
catcatcatc gtcaccacga cctggaaacg acacacgttc ccccccaatc ttgggcatgt 97860
gtatatatat aaagaatggg agggagagga cgtggggctc gagaagaaat aaacgccaag 97920
ctcgattcga accaaaaaac cacatgtgta ttgtgctttt tttgttttta cggtggggaa 97980
aaggaggggg ccgtcattaa cgaaaaccgt gtatggggtc cggacacgaa cagtacacag 98040
cttatgggga aaaaagctca cagagagaaa aaaacaccaa gctcaggcac gcgtacatca 98100
ttatcatcat cggatatctc accacgagtc atagtagtac caaggagtgt gtaacaccat 98160
tttttctttt ttctttgtaa cgggataagg gacagcaatc atcacgcaca acacccttca 98220
ctctcttttt agtcatccat atcatcgctg taacacagca tgtcctcgta atcgggcgtc 98280
tggcagcgca ttaccaccga gtcgtcttct tgcggtaccg gtggtggcgg cggcggcggc 98340
ggctgctgct gggttaccgt cgtactgtga ttaccgttgg cggactgcac cgggatgata 98400
ggctgcttgt ggggaacctg gggtggactg ccgccgtgag aaggcgacgg cgtcatcaag 98460
ttaagctcac cacggtgact ccggacaccg gcgaggggcc ccgggggact gggagggacc 98520
gcggtcgtct tgtagacgac ggtgtccccg tgtcgatccg tggctcgtac cagatcttga 98580
ctgctagcgt cgtcactgtc ttcgtcctct tccagctcgc cctcagagta gtgctgctgc 98640
ggttgcgacg gtggctgggc gggaggagcg gcggcgatca ttggagaggg atgtcgatga 98700
ctcccttctc tgtccttttt atcgtaggct gtcagcgttg ctgggtccgt cctgctttcc 98760
atatttgcgc attgctcatc ggtgggatga atttggtctc ctccccgctg ttgtccgccg 98820
gcagtggcgt ggttgctggc ggttgtcgtt gtcgtaccgg caaagacggt gagatccaat 98880
agtgactgct cgtcgaaggg acagtacgct atcatgaaac gatagggtgc caacgcgcgt 98940
tggatgcgca gttcgcacat ctcgttctga cactcgtggc actgcagggc gcctaggatc 99000
aggtccgaga cagcgccgca gcggtaggta cccatggcgt tgttagtatc gaactggtca 99060
aaaaattggg gcgtaccggt gacttgcaac gcgcgacggc gtagcgagac ggccacgcgc 99120
gagaaagagc acacgtaggc catggcgcgg tgcatgggtt gcgagaaggt ctcgggcgga 99180
cgcttctgca gatcgcagac gtcgtcgcgt agccaggcgc tcatttgacc tggctttttg 99240
actagccgtt tgagcgtgct gcaatggtcg ccccagccgt cctggtggtc caggatgcag 99300
cccaggtcca ggttgttgag tttgttgaag agcagctgac gcatgccgcc caccgtctcc 99360
agatagggat cgtgcgggtt gacgggtagc ccgtgcaggt ggtggtactt catgtagctg 99420
agcgtttcgt cgatgatggc cagcaacgtg tgcaagttgg gagcgttgta cacggcgaag 99480
atcttttcca ccaccagctt gcgcagcaac ggttcctcca gccaatcgaa ctgttgacgg 99540
atgtgcaaca ggtagtcggt gtgcatgagc tcgtcgtgtg acagcaggat gcgaccgcgc 99600
ggctgatgat cttgcgggaa ggcggtgggg accttgagat cggcggggta gggtgccaga 99660
cgtagactct cggccgtgta gcgctgaagg tcgtagacgg gcgaggtaga actcggtgag 99720
gtacccgacg aggcggcgcc gcgctgcaga cgcgctcttt tcttcttttc gatcaaacgg 99780
ctgagttgct gtagttcgtc ctcgtccatg gcgtccagtt cgtcgtcaat aagcgccagc 99840
atctgttgtt gttgcggtcc ggtggacgat ccgtgatgat tattggctga agaggggtga 99900
gaagaaccga aagtcgtagg acaactggga actcggcgac gaagatgcgt cgaatcgccg 99960
ccgtgatggt gcggttcgcc gtcatcgttg tcgtaagact taccgtagtg ggggttaagg 100020
ggcaccgagg cggacgcggc cacgcgtcgc ttgaaagagg aggacgccct atgtccgcca 100080
cggaagcccg cggtgcccat gatgatgtgt ccgccggtgc ccccgagtgc gtggcgggag 100140
gagggtggaa ggggaggagg atagtggtcc ggatcgcctt cggtatcatc gtctttgctg 100200
tagcggggtc gtcgtgcggg gacgcagggt cggtgatgat gcgaggcggc gccgacggta 100260
tcttccgcga gatggtattc gctggcggct gctccgttcc gtgtcgacgg cgaggttgga 100320
cttcgctcgc gtcggaactt ccgtggcacg ggttcgtaat ctagacagaa gcgccgtgcg 100380
cgacgggcgc ggcgttcgcg ctcgctcagg gaagataacg acggagcgtc gtgacggccg 100440
cgtgagtgca gctccatggc cgccgtcgct aggaaggtca cgttcgggca cgctgatgta 100500
tatatagatg agaccgctgc cggggggcgg gtcaccggcg ccgtggaaag tgaggctcag 100560
acggcggtcg ccggcggcat gggcgcgtcg ggcggtctga ttttgatgga aatgtggacg 100620
tttttggcgt tggagtgaca ctttttggtg aaacagcggc tccagaggct ggcccagagc 100680
gcgtagctgt gctcggtgcg caggtcgatg aacacctgca cggtctcttg cgggttgcgg 100740
tgcgtgtagt tgagacagcg aaaatcccgc gtgcgcgcgc cgtcgcgccg cttgacggcc 100800
acgcagcagg cgccgtgggg ctgaaagagg aggacgtggg gtgcggtaaa ctgctcgctg 100860
acgtgcggct cgtagtgttg cgtgaggtgc tcgagcagcg gcggccacac gcgggtgacg 100920
acgagccgct gcaagtccgt gtcggaaatc gcagcggcag tggcgccgtc gccaccgtac 100980
aggtgatagg cgagcacctc ggtgagaccg cggcgtcgat aacgcgtcac gttaagcgag 101040
cgcgtctcga taaagttggc ttcggtcgag gggcagattt tgtcgcgcac gctgagaatg 101100
acgcgtggcg gcggcgacag gggcaacgcg ggcaggtcgt gcggcgggtg gtggtgaagc 101160
aggttacgca gatccagttg ggcgcgcaca aagcctagcg ggtgttcgcg gtaggcgtcg 101220
ggcacgatga acagcggcaa cagacggcga tgcatgaaat agccgtcgtc ttggtccatt 101280
ttatacatgt agggcagacg tacagagcgt ccatggtggt agatgcctgt gtctaggctg 101340
ctctcgggat gcgagatggg gtccagcagc gtgtgcagtt cggcgtcgag acagacggcg 101400
tgattgagca cctgcgccac ggcgcgtaaa acgctggggt gtacggctac ggtgcaggcg 101460
gggaacggcg tgatgatgcg cagccccagt ttgcccttgc agcggcagta agggggtgac 101520
gtgtcaacgg aggacgttgg tttttgaaaa acgccgttat cagggacgtt atttttgtcc 101580
tctttcccgt cttcgtcttc ctctgtgtcg cgctcgtccc ggtaatcgag atagtcgtcg 101640
tcatcgaaag gcgcgccggc cgcgtccacg ggcacgctgt tgggtgggca cgcgcttttg 101700
aagaaataga ccgggtgccg gtcggggtgc gtgtagccaa agaggctcgc ccatacggtc 101760
atccagacgc gtcgtagtcc gcgacataat tcaaagacgg tgtgtcgcgc cagaccggag 101820
acgccgtcgc gcagtcgtaa atcaaagtcg gccacaaaat tgaagacggg cagacgttcg 101880
ttgaagactt cgtgtcgcgt gtagtagaac tgtgtctcgg ggctggtgct ggccacgtcg 101940
tcgtcgtgta gccacacggt ctcggtcagg gcctcgtccg agaaacggct gtcgggtacg 102000
tgacggagca ggtcgcgcgg aaagaggctg cgatgccagg tttcggaggc cacggcgcag 102060
aagacgtgct ggtcattggg caggtgtacg cggtagacgg gcagcggtcg ctccagcagc 102120
ggtgccagcg cgggctcggg tagcaggtag cgacgttgcg agtaacgcgt tagcgtgccg 102180
gtggtgtagg tctgggctgt gcgcagcgag gcgcatagac gtaacaagcc ggacagggag 102240
cgttccagtg gcgagaagac agactcggaa agcgtgttga tgcgttcgag ctggcgcgcc 102300
agctgcgtgg aggtgccaaa gaagcccgcc aggtgcgtgc cgtcgatgcg gccgccgtag 102360
ccggccagcc ccaggccgtg cgggctggtc gccgagtggg gggattcgtc gagacccagt 102420
aggtgcgtct ccacgtagtc gtgcagaaag ttgtcgagcg agaagtattt ttgcatgacg 102480
tccagcagct cggtggaaag ccggcggccc agaaaacccg gttcgcgcgt gcactgcgct 102540
tcgggcgccg cgtcagcgtc gtaagccacc acgcgccggt actcgagcaa ccgcgcgcgt 102600
gccagcgccg tgcggtaggc caggtagacg tagtgcacgc agaccgtgtc gggcagacgc 102660
gcacgttcgc ggaacgcgtt gatctgcgtg tccacctgct ctagctcggt gtagtcgcgg 102720
cggttgcgcg ccacggcgta cgccacgaaa gcggacacgc gctgacggaa gggcgagccc 102780
agtagcagac gcgcgaactc gcccatggag gcgtgcgtgg ggatgatggt gcccaggtcg 102840
cgcgtgcaga agctgcgcac gtactcctcc acggtggaga tggtgctgta ctggccctcg 102900
aataggtagt aggccatggt cagcagcacc tggccctcgg tgtgcccgaa gacgctgatg 102960
aaccacgagg gcgaggtggg gcagaggaag acctggttga gatgacgtag cacggccgcg 103020
tggtgaaagt acaccaggtg cttgaattcg cgcacctcgc cgccgtgttc gggcgagagc 103080
acgggcgtgc ggaagagatg ccggtagagc ggttgcgtct cggcctcgtc cagactggcg 103140
atgagcgccg agagggggat gggctggcgc gcggccaggt agcgcgagag ctgcagcgtt 103200
tcgttgttca cggcgaagac gggcgccacc cgccgcgagt ccgagcactt ttgcgtttgt 103260
aggcagaaat aaacacgtcg cgagacctgg tgtttgacca gcagggggaa gacgcagtgg 103320
tccgtcggtg tctgcgagag tacgttggcg actatatgag cagaatcata ctctgttgcg 103380
aacagaacga gcgtcatcgt cgcgccggca cgatgcagct ggcccagcgc ctgtgcgagc 103440
tgctgatgtg ccgtcgcaaa gccgcgcctg tggccgatta cgtgctgctg cagcctagcg 103500
aggacgtgga gctgcgcgag ctgcaggcgt ttctggacga gaactttaag cagctggaga 103560
tcaccccggc cgacctgcga accttttctc gcgacacgga cgtggtgaac cacctgctga 103620
agctgctgcc gctctatagg caatgccaga gcaagtgcgc gttcctcaag ggctatctct 103680
cggagggctg tttgcctcac acgcggccgg cggccgaggt ggagtgcaag aaatcgcagc 103740
gtatcctaga ggccctggac attctcatcc tcaaactggt ggtgggcgag tttgccatgt 103800
ccgaggccga cagcctggag atgttgctgg acaagttctc cacggatcag gcctcgctgg 103860
tggaggtgca gcgcgttatg ggcctggtgg acatggactg cgagaaaagc gcgtacatgc 103920
tcgaggccgg cgcggctgcg acggttgcac caccgacgcc accggcggtc gttcaggggg 103980
aaagcggcgt ccgcgaggac ggggaaacgg tcgccgccgt gtcggccttt gcctgtccct 104040
cggtttcgga ctcgctgatc cccgaggaaa cgggggtcac gcgtcctatg atgagtttgg 104100
ctcacattaa caccgtctcc tgtcccaccg ttatgaggtt cgaccagcgg ctgctggaag 104160
agggcgacga ggaggatgaa gtgaccgtga tgtcgccgtc acccgagccc gtgcaacagc 104220
agccgccggt cgagcccgtg cagcagcagc cccagggacg cgggtctcac cgtcggcgtt 104280
acaaggagtc ggcgccgcag gagacgctgc ctacgaatca cgaacgcgag attttggatc 104340
tcatgcgaca cagccccgac gtgcctcggg aggcggtgat gtcaccgacc atggtcacca 104400
tacctcctcc ccagataccc tttgtgggtt ccgcgcgtga actcaggggc gtgaagaaaa 104460
agaaacccac ggcggcggcc ttgctgtcct ccgcgtgaac agcctggcac gttttggaaa 104520
acgtacgtga tcacggacac gacgagcacg gggtttctca tagacgtact ttattaggtc 104580
agggatgacg gggaggtttc gggccgacgt caaaaataac gtcactcgtg ttgacagggc 104640
tttctgcgtc ggagctcttt tcatcttctt ctgtctcgtc gacgtcatcg tctaccggcg 104700
agggtgtccg ttgcagcaac gcgtgctcgg gcgtgtgggt gaaaccgatg tcgggggtgg 104760
gcggcacgat catctgtcct agggggtgac tgcccaccgg cagataggta aagcggtggg 104820
tggtaaaaac cgctttggct acggtggtgt gtggggagat gcagacggtg gtgtgcgaag 104880
tgttgaccac cgtcacgccg gccgcggtac ccgggagcca gatggtgggt cgaatgatga 104940
gatccgattg actaaactgg cgcacgccca ctatgagggc gcagataccg ggcgcgtgca 105000
cgtaggccgc gtcaaaatag acggtttgcg tgtgacccgg accgatcacc agcgtctgac 105060
gggtacgtaa tgaaaagaaa cggtgttcgt tgggcggcgg caagttcatg agctgccagg 105120
gttctggcac aaaacagggg aaaacgccga tatcgccttc gatggtgccc ggaaagatgg 105180
actgaaaagt gtcgttgagg ttgacgacat ccaactgcgg gacttgcagc ccggattcca 105240
gcagctcggg catgcaaacg aattgcgcgt ccaggcattt gtaaaaggta atgccaaaaa 105300
aaccttcggg gatatagagg ctgactccca gcgaggtggg cactttgcgc tcgcgtgata 105360
gccaaatgat gtgtttattg taaaaagcca gctgcgtgtg gcattgttta acgatgaaac 105420
tggaaggcat ccacttgtag ggaactttga gcggcgacgg taatggcgac gacgcttcat 105480
cctctcccgg atgctgctct ttgtcgtatt tctcctcggt cgattggggc agcgtaaatg 105540
tggtttgaaa atcgctatcg ctagcgaaac gcacgcagta acgcatgttg acggatttct 105600
cggctaggat gatggagcct gatgacggtg cggactcttc cttcattatt aacgtagggg 105660
tctcccagaa tcgctgaaaa cgggagcgcg gcagccgcga cagtaccagt tgagagtcga 105720
ttctgtcggt caacattgta agcatcgtgg cggtggtgtg atggagtgga aaacagtgat 105780
actaggtgtt tttgttttat cggtggcagc ggggagttct ggtaacagct catccacgtc 105840
aacctccgca actacgttaa aatcgtccag ttctagcgtg tcaacaagca aattgacgac 105900
aactgcgaca actacgacaa ctacgatgag tacgacctca tcgacaacta ccactaaacc 105960
aagttccact actcacgacc ctaatgtgat gaaacgacat actcacgatg atttttacaa 106020
ggcacattgt acatcgcata tgtatgagct ttcactgtcc agcttcgcgg cttggtggac 106080
tatgcttaac gctctcattc tcatgggagc tttttgtatc gtattacgac attgctgttt 106140
ccagaacttt actgcaacca ccaccaaagg ctattaaggg tggacagatt tacagctcga 106200
cggtgttccg gcggggtaag gtttccataa gtgggtgact ggagactaaa gttacggatc 106260
tcatctagaa atagcagcga gtctagatag tcccacaggg gatctataaa cgttctctga 106320
aatcccgttg atggtgacgt aggtgtagtt tcggaagccg ttttgttttc cacgaacatg 106380
gtttcgttgt aatataagga gctcatatca agagtaccgt aaatagtgta cggtgtttca 106440
ttacggatta gtacatgcgt gtttttcata aattctgata cggcggttcg gttgcggctt 106500
gattcacaaa aagggttttg ccggtaacgt agagtggtat acacccacgt cgctaggtcc 106560
cttaattgcg tggtcataat ggacttcata aagctactat caggacgata agcaattgta 106620
gacgtggaaa cccgccttgc ggtggtagta acactataag ttgcgttagt agtgacgttc 106680
agagcggttg acgttgtata gggagaatat ggcgtagtag tactttgaga tttcttactc 106740
tttttttctg attgttcttt gactggagct tgtttacgct tgagttttcg catagtgttt 106800
ttcaacttag taccgttaat atacttaggg acgcgaaata aatttcggct catggcgtta 106860
accaggtaga aactgtgcgt acagttgcgt tgcgcgtaac gtagaagcaa ggcggttagg 106920
cctaaaaagt agatcgtttg actatccacg tttactttct tggaacctac atataacttc 106980
gtgttccaac gtggcacatt gaaaaacatg gggttgaacg tggtgaaatt gccgcagcct 107040
tgttcgccag tatcattacg tttggaaacg tttagcattt cggaaagaca agtcatggaa 107100
ggcaccgtac cgcaagatgg gggtctgaat gttattgttt tagccgtatg attgtattct 107160
gagaaaacgt acttagccgg ttttcgaagc tgagtgctat aaaaatcgaa ccaaagatag 107220
gtaacactgt tattttgaat gggtcccgct aaaatgtaat accgtggaaa ctcggtcatg 107280
ttcatagtca gatttttaat gtgttgtctg gtcatattaa agtattttgt atagatatcc 107340
ttttctagtt gtttcaaaat ctctaatttg aacttgtcta gtctttgctt gcctatcgta 107400
gacagtactt tacctgacca gtaacgtcct acggataatc gtaccgcagc cctacagttt 107460
atgaaagaga atagcaggaa agttagcgac ataaggaaga ataaattaaa aacacctctc 107520
atctctcctt ttctccccat gacagaggag gagaccccgc accgtccgtc tgccttgtgg 107580
tttggcttgc ctgcgtgtac tcactgctga ttctggtcgt tttgctgctc atctaccgct 107640
gttgcatcgg cttccaagac gacctagttt cccgcacctt ggctgtgtac cgagcttgta 107700
tccagggacc gatatgtaac cagacccaca acagtacctc gtaaataaag acgcacacac 107760
ctcacgcata tagtaccatc acaccgtgtg gcgtgtactt tattacaacg agcaagagtg 107820
ccccctaact attggggccc gtaccgtttt agaaggtttt gtgtgaatgt ctttaacttc 107880
tctgtccctt ttctcataaa ctgtcaggtc ctacagtcag catgtcttga gcatgcggta 107940
gagcagatag atgccgatga tggccgatag cgcgtagacg gacatcatga ggagacgact 108000
gtcggtggcg tccacgacaa cgtcagttac ttctaggacc gtaccgtttt tcaaaagcat 108060
gaggtagtga gttcgcggag atgagaccac cacttcgttg tagggatcca gggcgaaaag 108120
gacgtcgtcc gagtcgtgca tgtacatgat gttgatgacg ccttgcgtgt cgtcgtattc 108180
tagcagggcg ctttggcaaa aggcgcagtt ttctagggaa atgttgagcg ccgctgtgat 108240
gctgtgtgtg gtatgcatgt tgcgcgtcag ttcgcattta gtttgactgt ccgtctgggt 108300
gatgatgagg ctttggccta cgacggtggt ggagacaggg taggagatac ctttgatcag 108360
gtactggttt gttacgacat aactgacgtg ttcggagacg gtcagcgcgg agaaggattc 108420
gcctagtggc agacaaaaca ggtcggggaa ggtttccaac gtgcttggtt gcatggtaga 108480
taggatggag agggcggcgg gaacggtagt ggggacggtg gcatcgggga agagacgtgt 108540
gaggcgttcg agcgagtgat cgcgtcgccc gctactggaa cagggtgtgt acaggtcgct 108600
gaggtattcg tggtgcggat gagctagcaa ctgcgtaaag tgtgatagct cggccaatga 108660
acagaggccc gtttctacga tgaagatttc gcgtctctcc gtcgtatgta ccagcatgga 108720
gtggacgagg ctgcccatga ggtagagttc ttgacgcgcg aaggctgaaa gaaaagaggc 108780
caggtgcgtt ttgtgtagtt ttagggcaaa gtcggcgatc tgtcgtagtg cccactgggg 108840
gatgagatgt tgctgattct gtttagagag tatgtagacc aggcgtacga ggctggtgat 108900
gtcggtgatc tgattcggtg tccaaagggc tcgtttggcc aggtccacgg ccgtgggata 108960
cagtagcaac gtggtgcgtg gtggtgtttg tgagaggcag gtgatcataa attcttgtat 109020
ttgtaagagt gcggcctggc ggtctagggc ccgtgggacg gagacttggg cgccggcctc 109080
ttcttgtcgg gctgctgcga acagtgctaa tgcgtaggcg aaggccattt ctaccgtgcg 109140
gcggtccagc atctgacatc gaccgctctt gagtacatcc acggcgtaac ggtgaaagct 109200
gttacgtagt agtgcgctga ggtctaggta gttgaagtca agtgcggcgt caagaaagtc 109260
cgggtctttg agataagagt gacggttcag ttgatctttc ttaactagca ccaggagctc 109320
gtgtttttca gtttgtcgta gtataaagtt gtcgcgttga tagggcgctt tgaaaagtac 109380
gcgtggaaga tggccgaaga taagcagcat gggtgtgtcg tcgtctatgg acaccgtaac 109440
tacgaagaag tcctcggtca gtgtgatttt aacgtaacgt agttcgtcga tgaggtaaaa 109500
gccttggtgc aaacaaggtg tgacggtgct gaatagtaga tcgtgtccat caaagaggat 109560
acaggtctgg ttaaagtgtg gtcggtgtag tcctgaggtg gtatgtgatt ctgtccagcc 109620
gtgtggagtg gtttgcggtg gcatccaaac gtgaggtatt gacaggtcaa tgggcggtgg 109680
cacagtggtg ggctgttcac ctaggctgtc ttgtgccttt agctgctgcg aaaaagatcg 109740
gtagctggcc aggtctttgg ataccagcgc gtaagtgtta agtctctgtt ggtatctttc 109800
cagggtttcg gtcagatcta cctggttcag aaactgctcc gccagaggac ccgcaaaaag 109860
acatcgaggc atatggaata catagtattg attatagctt tggaaaaagt tgaaactgat 109920
ggcgttttcc ctgacgaccg tgctgttacg gaggctgctg ttgtaggtgc actgggtggt 109980
gttttcacgc aggaagcgga tgggtctccc gtaggtgttg agtagtaggt gaaacgcgtg 110040
agggtccagc gcttcggatg cggcgtctgc gccatatcgt tgcgaaggta ggtgactgag 110100
gaggtagacg gcgaagacgg tgaggtagga ggggaggccg ggccgcatag cgcggccgcg 110160
ccgctgggtt cagcggcgtg atccaggtgg tggttggcgt tacacccgag agaaggagaa 110220
aaaggatccc aggaaggagc acccgggtgc ggcgctacgg gttacaaaag tcgcgtctcc 110280
gtctatttaa tacgatgtca ttggccgctg cgaagggaga agaggggaca cgcgaataag 110340
ccatgccgtc cgggcgtggg gacgacgctg atttgacggg gaacgctctg cggagattgc 110400
ctcacgtgcg taagcgaatc ggtaagcgca agcacctgga catctaccgt cgtctgctgc 110460
gggtctttcc ctcgtttgtg gcgctcaacc gcctgttggg aggccttttc ccacccgagt 110520
tgcaaaagta ccgtcgccgt cttttcatcg aagtacgatt aagtcggcgg attcccgact 110580
gcgtgttggt gtttttaccg ccggactctg ggtcgcgcgg catcgtgtat tgctacgtga 110640
ttgagttcaa aaccacgtac tcagacgccg acgatcagtc cgtgcggtgg cacgccaccc 110700
acagcctgca gtacgccgag ggcctgcgcc agctcaaggg cgcactggtg gactttgatt 110760
ttctgcgtct gccacgcggt ggcggtcaag tctggagcgt ggtgcccagt ctggtttttt 110820
ttcagcaaaa ggccgatcgc ccatcctttt atcgggcttt ccgctcaggc cgttttaacc 110880
tgtgtaccga ttctgtcctg gactatctag ggaggcgtca ggatgagtct gttgcacacc 110940
ttttggcggc tacccgtcgc cgtcttcttc gagccgcacg aggaaaacgt gctgcgctgc 111000
cccgagcgcg tgcttcggcg gttgctggag gacgcggcgg tggcaacgcg cggcgggggc 111060
tggcgcgagg acgtgctcat ggaccgggtg cgcaaacggt atctgcgtca ggagctcagg 111120
gatctgggtc acagggtgca gacttactgc gaggatctcg aagggcgcgt gtccgaggcg 111180
gaggcgttgt tgaaccagca gtgcgagctc gacgaaggac cgtcgccgcg gacgctgcta 111240
caaccaccgt gtcgtccgcg ttcgtcgtcc ccagggaccg gcgtggcagg agcttccgcc 111300
gtcccacacg gtctttatag tcggcacgat gccatcacgg gacccgccgc cgccccgtct 111360
gacgcggcga ccgcgtcagc ggccgccggt gcttcttcta cctggctggc gcagtgcgcc 111420
gagcggccgt tgcccgggaa cgtacctagc tactttggaa tcacgcagaa cgatcccttt 111480
atccgctttc acaccgattt tcgtggcgag gtggttaaca ccatgttcga aaacgcctcc 111540
acttggactt tctcctttgg tatctggtac tatcggctca agcgggggtt gtacacgcaa 111600
ccacggtgga aacgagtgta ccatctggcg cagatggaca acttttccat ttcgcaggag 111660
ctgctgcttg gcgtggtcaa cgctttggaa aacgtgacgg tgtatccgac gtacgactgc 111720
gtactctccg atttggaagc cgccgcctgt ctgctggccg cctacggaca cgcgctttgg 111780
gagggccgcg atccgccgga ctccgtgacg gcggtgttga gtgagctacc tcagctgttg 111840
ccgcgtctgg ccgacgacgt gagtcgtgag attgccgctt gggaaggccc cgtcgccgtg 111900
ggtaacaact attacgcgta tcgcgactcg cccgatctac gctactacat gcccctaagc 111960
ggtggtcgcc actatcaccc gggcactttt gatcgtcacg tgctggtgcg gcttttccac 112020
aaacgcggcg tcctccagca tttgccgggc tacgggacga taacggagga gctggtgcaa 112080
gagcgtctgt cgggccaggt gcgcgacgac gtgctttctc tctggagtcg acgtctgctg 112140
gtcggcaagc tgggtcgcga cgtgcccgtc tttgtgcacg aacagcaata tctgcgttcg 112200
ggcctgacct gcctggctgg cctgctgttg ttgtggaagg tgaccaacgc ggatagcgtc 112260
ttcgctccgc gcacgggcaa atttacgttg gccgacctgc tgggttcgga tgccgtagcc 112320
agcggcgggt tgcccggggg gcgcgcgggc ggcgaagagg agggctacgg gggacggcac 112380
gggcgggtac gtaactttga gtttctggtg cagtactaca tcgggccgtg gtacgcgcgc 112440
gaccccgcgg tcacgctgtc gcagctcttt cccggcctgg ctctgttggc cgtgaccgaa 112500
agcgtgcgca gcggctggga tccctcacgt cgcgaggaca gcgccggagg tggcgacggc 112560
ggcggcgccg tgctcatgca gctcagcaag agcaaccccg tggccgacta catgttcgcg 112620
cagagctcca aacagtacgg cgatttacgt cgcttggagg tacacgacgc tctgctcttt 112680
cactacgaac acgggctagg gcggctgttg tcagtgaccc tgccgcgtca tcgtgtgtcc 112740
actctgggct cgtccctctt taacgtcaac gatatttacg aactgttgta ctttttagtg 112800
ttgggttttc ttccgagcgt ggcggtgttg taatttccac cacgtgtcgc tcgctgcata 112860
aagggcgagc gtccccggag agggtatatt cgtttggcga gagcgggcag cggtggtggg 112920
tatgtcccct tctgcggaga agactacctc agtcaccgat tccatcatgc tcgctatcgt 112980
gaatttcaaa tacatgggcc cgttcgaagg ctactctatg tcggccgatc gcgccgcctc 113040
ggatctactc atcggcatgt tcggctccgt tagcctggtc aacctgctga ctatcatcgg 113100
ttgcctctgg gtgttgcgtg ttacgcggcc gcccgtgtcc gtgatgattt ttacttggaa 113160
tctggtactt agtcagtttt tttccatcgt ggccaccatg ttgtccaagg gtatcatgct 113220
gcgtggcgct ctaaatctca gcctctgtcg cttagtgctc tttgttgacg acgtgggcct 113280
atattcgacg gcgttgtttt tcctctttct gatactggat cgtctgtcgg ccatctctta 113340
cggccgtgat ctctggcatc atgagacgcg cgaaaacgcc ggcgtggcgc tctacgcggt 113400
cgcctttgcc tgggttcttt ccatcgtagc cgctgtgccc accgccgcta cgggttcact 113460
ggactaccgt tggctaggct gtcagatccc tatacagtat gccgcggtgg acctcaccat 113520
caagatgtgg tttttgctgg gggcgcccat gatcgccgta ctggctaacg tggtagagtt 113580
ggcctacagc gatcggcgtg accacgtctg gtcctacgtg ggtcgtgtct gcaccttcta 113640
cgtgacgtgt ctcatgcttt ttgtgcctta ctactgcttc agagtcctac gcggtgtact 113700
gcagcccgct agcgcggccg gcaccggttt cggcattatg gattacgtgg aattggctac 113760
gcgtaccctt ctcaccatgc gtcttggcat tctgccgctc tttatcattg cgttcttctc 113820
ccgcgagccc accaaggatc tggatgactc ctttgattat ctggtcgaga gatgtcagca 113880
aagctgccac ggtcatttcg tacgtcggtt ggtgcaggcg ttgaagcggg ctatgtatag 113940
cgtggagctg gccgcgtgtt acttttctac gtccgtccga gacgtcgccg aggcggtgaa 114000
aaagtcctcc agccgttgtt acgccgacgc gacgtcggcg accgttgtgg taacgacggc 114060
cacgtctgag aaagccacgt tggtggagca cgcggaaggt atggcttccg aaatgtgtcc 114120
tgggactacg atcgacgttt cggccgagag ttcctccgtc ctctgcaccg acggcgaaaa 114180
caccgtcgcg tcggacgcga cggtgacggc attatgagcg gcggcgctgt acgccagcgg 114240
ggagaaaagt ggcagataaa tcacgtcagg ttcacacgtc gttagccagc gtcggcatat 114300
gaagggcgcg ggcggccagt acggcctctg ggctgagaca ggacgaggca gggtgagaaa 114360
gaggaggatg ggggggaccg gggtggtggt gctgctgctg ttgtgggtgt ggacggtgcg 114420
gatgccggga cagcgtgccg gcgaacgttc tgtaatcttc cataataaag gtaaaaatgc 114480
ccgtctcgtg tcgactccgc tggatctcga aggcgtcggg ggtaatgcgc atcttgccgg 114540
tgccgatgag ataaaagtac cacatttttt gacagatgat gcgaatcaag ggttcgtacg 114600
cttcggcacc ccagtggcgc gtgaagaagg ccgccagacg aaacaagcgg tgtccgtaga 114660
gcgtgcctag ggagaagagg atgttgccgt tgcgcgccag gtcttcgggg aaaacgaccg 114720
gcaggccggt gtggcgctgc acaaagcgcg tcagcagtcc gccgctcaag cgcgggtgac 114780
acaggcgctg gctgagacgg gcggcgcgcg tttcatcgaa cacggccgcc tcaaagtcca 114840
gccccgggaa ggcctgacgc agttcgcggt acagatgagg ccagtagggt tgcggcgtct 114900
tgcgactaag cacggcgtgg tccgagacgc ccaggttgtt catggtttcg cgcagtagca 114960
gcgtttcgag accgcggtga aagaggagga cgcagatgag gcgtacgatt ttgagttctt 115020
ccaaacgcag cgagctcagc ggctgtccgc gcgacatctt ctcgctaatc tgtaatatta 115080
gatgattggc gcaagtaaag gagaatttgc ccgtgcggac ccgcgggacg gcggggttct 115140
cttcgtcgcg ggccatcatc gttcgctcgg tgagcgggta gcgacggtga cgacaatgac 115200
gatggacgag cagcagtcgc aggctgtggc gccggtctac gtgggcggct ttctcgcccg 115260
ctacgaccag tctccggacg aggccgaatt gctgttgccg cgggacgtag tggagcactg 115320
gttgcacgcg cagggccagg gacagccttc gttgtcggtc gcgctcccgc tcaatatcaa 115380
ccacgacgac acggccgttg taggacacgt tgcggcgatg cagagcgtcc gcgacggtct 115440
tttttgcctg ggctgcgtca cttcgcccag gtttctggag attgtacgcc gcgcttcgga 115500
aaagtccgag ctggtttcgc gcgggcccgt cagtccgctg cagccggaca aggtggtgga 115560
gtttctcagc ggcagttacg ccggcctctc gctctccagc cggcgctgcg acgacgtgga 115620
ggccgcgacg tcgctttcgg gctcggaaac cacgccgttc aaacacgtgg ctttgtgcag 115680
cgtgggtcgg cgtcgcggta cgttggccgt gtacgggcgc gatcccgagt gggtcactca 115740
gcggtttcca gacctcacgg cggccgaccg cgacgggcta cgtgcacagt ggcagcgctg 115800
cggcagcact gctgtcgacg cgtcgggcga tccctttcgc tcagacagct acggcctgtt 115860
gggcaacagc gtggacgcgc tctacatccg tgagcgactg cccaagctgc gctacgacaa 115920
gcaactagtc ggcgtgacgg agcgcgagtc gtacgtcaag gcgagcgttt cgcctgaggc 115980
ggcgtgcgat attaaagcgg cgcccgccga gcgttcgggc gacagccgca gtcgggccgc 116040
cacgccggcg gctggggcgc gcgttccctc ttcatccccg tcacctccag tcgaaccgcc 116100
atctcctgtt cagtcgcctg cgcttccagt gtcgccgtcc gttctccccg cggaatcacc 116160
gccgtcgctt tctccctcgg agtcggcaga ggcggcgtcc atgtcgcacc ctctgagtgc 116220
tgcggttacc gccgctacgg ctcctccagg tgctaccgtg gcaggtgcgt cgccggctgt 116280
gccgtctcta gcgtggcctc acgacggagt ttatttaccc aaagacgctt ttttctcgct 116340
acttggggcc agtcgctcgg cagcgcccgt catgtatccc ggcgccgtag cggcccctcc 116400
ttctgcttcg ccagcaccgc tgcctttgcc gtcttatccc gcgtcctacg gcgcccccgt 116460
cgtgggttac gaccagttgg cggcacgtca ctttgcggac tacgtggatc cccattatcc 116520
cgggtggggt cggcgttacg agcccacgcc gcctttgcat tcgtcttatc ccgtgccgcc 116580
gccaccatca ccggcctatt accgtcggcg cgactctccg ggcggtatgg atgaaccacc 116640
gtccggatgg gagcgttacg acggtggtca ccgtggtcag tcgcagaagc agcaccgtca 116700
cgggggcagc ggcggacaca acaaacgccg taaggaagcc gcggcggcgt cgtcgtcgtc 116760
ctcggacgaa gacttgagtt tccccggcga ggccgagcac ggccgggcgc gaaagcgtct 116820
aaaaagtcac gtcaatagcg acggtggaag tggcgggcac gcgggttcca atcagcagca 116880
gcaacaacgt tacgatgaac tgcgggatgc cattcacgag ctgaaacgcg atctgtttgc 116940
cgcgcggcag agttctacgt tactttcggc ggctctcccc gctgcggcct cttcctcccc 117000
aactactact accgtgtgta ctcccaccgg cgagctgacg agcggcggag gagaaacacc 117060
gacggcactt ctatccggag gtgccaaggt agctgagcgc gctcaggccg gcgtggtgaa 117120
cgccagttgc cgcctcgcta ccgcgtcggg ttctgaggcg gcaacggccg ggccctcgat 117180
ggcaggttct tcttcctgcc cggctagtgt cgtgttagcc gccgctgctg ctcaagccgc 117240
cgcagcttcc cagagcccgc ccaaagacat ggtagatctg aatcggcgga tttttgtggc 117300
tgcgctcaat aagctcgagt aagagagacg ctatatttag ggtttccctc tctttttttt 117360
ttctacaccg tgatacccta ataaagcaca ctgcggttat tatcaacgtc tctgtgtttt 117420
tattatttag aaataaatac agggaatggg aaaaacacgc gggggaaaaa caaagaagtc 117480
tctctctact ggctcagagg atcgttgccg aacagggact tcagggacac cagggggggc 117540
acctgctctc tgtccttctg ctcctgcttg ggcaggctgg cgatgccctc gcccatgccg 117600
aacagctcgg cgggaggggc ggtgggctcg ggtctgctct gggggaaatt gccgggtctg 117660
cccttgctgc taggccaaat cttgcccagg aaattggcct gcctctcggt gcagtccttc 117720
atctggtgcc cttccttgcc acacttccag cagcccttct tcctgggggc tctgcagttt 117780
ctggccaggt ggccctcctt gccgcagttg aagcacttga tccgcttctg gcctctgaag 117840
ttgccccgct gcatcatgat gttggtctgc tgggcctggc tcatggcctc ggccagcact 117900
ctggccttgt ggccgggtcc gcccactccc tggcaggcgg tcatcatctc ctccagtgtg 117960
gcgccgctgc ccagggcctt caggatgctc ttgcagtcgg ggttggcgtt ctgcaccagc 118020
agggtctcgg tcatccagcc cttcacgtcc tgggtggcct gctcggctct cagggccttg 118080
aagaaccggt ccacgtagtc tctgaagggc tccttggggc cctgcttgat atccaggatg 118140
gacacggggc tgtacatccg cacaatcttg ttcaggccca ggatgatcca ccgcttgtag 118200
atgttgccca cagggatggg agggttgccg gtcatccact gcagctgttc ttgaggggtg 118260
gaggtggtgc cggcgatgtc gctgcctctg ggctctctga tctggccagg ggggatgggt 118320
ccggcctgca cggggtgcag tctgtcccac tcggcggcct cctcattgat ggtgtccttc 118380
agcatctgca tggcggcctg gtgtccgccc acaatgttca gcatcacgtt caggtcctgg 118440
ggggtggcgc cctcgctcag ggcgctgaac atagggatca cttcggggct gaaggccttt 118500
tcctcgatca ctttcaccca ggcattcagg gttctggggc tcaggttctg gtggatcatc 118560
tggccctggg cattctggat gatggggtag ttctggctca ctttgctgct gtcgccggtg 118620
tcggcagcgg cctgctgggt tttctgcttg ctcttgttct ggatctcctc gatcttgtcc 118680
agggcctcct tggtatcctt cacgtcgatc cgctggtgca cgcagtacag ggtggccacg 118740
gtgttgaaca ggctcttgat ctcctcggtg ccggttttca cggcgggctg cagctggttc 118800
atgatctgct ggcagccctc ggtggtctcc agcaggctag gattcagggc gaagcgatcc 118860
agctctctgc tggcccacac caggtgcttc agccggtact tcttcttgcc gccaggcctc 118920
agtctgatct tctcccaggc gtccagcttg cccccgctca ggatgctggc tctggcggcc 118980
atggtggctg cgaaggcggg atggggggag ggtcagggga tgcgcaaagg tgaacgggtc 119040
ttcgtgggag gtcgggaagg gttccggcaa ctgtcgcaaa tatagcagcg gcgacaggtg 119100
tggcgcccaa aagtcgcgtg tctgagtgga cgtgggtttt tatagagtcg tcttaagcgc 119160
gtgcgcggcg ggtggctcaa cctcgatgct ttttgggcgt cgaggcgatg catggcccgg 119220
gcagggcttc ttgccggtgg cggcgacgtt tgggttgcgc agcgggctgc catacgcctt 119280
ccaattcggc gaagatgcgg tagatgtcgt tggcgtccca gaagaactcc tggtacttca 119340
gattctgacc ctgaaccgta gccaccatgg gcaccaggtt gcgggccagg atgccggcct 119400
gccagggcgg ccaggtgaac acggccggat tgtggatttc gttgtcggaa tcctcgtcgg 119460
tgtcctcttc gggcgcgacg gtggactcgg ccttaaggcg gccgcgtgtc ataacgcccg 119520
ccgtgcacgc cgtcgccgag gatgctgatt tgcgtttgcg gcccgcggaa gtggaggcgc 119580
ccgccatggc gccgccgccg gtaacgcggg gcgtcttgcg ctcggtggtt acgagttctt 119640
cgtcggagtc cgatccgctg gtccagacgt cgtcgtcgcc ctgggcggca ccctcgtcgt 119700
gccggtccca ggtgtgtcgg tactcaagct tgccctggat gcgatactgg ctggtgaagg 119760
tggggtgctc gctgtactga ggcccgcgct gcagcagcaa gtcgatatcg aaaaagaaga 119820
gcgcagccac gggatcgtac tgacgcagtt ccacggtctc gcgtatcgct tgtacctcca 119880
ggaagatctg ctgcccgttc atcaacaggt tacctgagat gctcaggccc gggatgctct 119940
tgggacacag cagcccaaaa tgctcgtgtg aggtaaaagc cacatccagc atgatgtgcg 120000
agatcttgcc cggtttgatt atcatatttt tgggacacaa caccgtaaag ccgttgcgct 120060
cgtgggggcg catgaagggt tgcgggttgc gggtcatcgt caggtcctct tccacgtcag 120120
agcccagcgt gacgtgcata aagagcttgc cggagggcac gtcctcgcag aaggactcca 120180
ggtacacctt gacgtactgg tcacctatca cctgcatctt ggttgcgcgc gtgttctcca 120240
tggagcaaac cagctcgtgc gcgcacacca cgtgccgcag tgccacgtcc ttggtgggaa 120300
acacgaacgc tgacgtgtag tagacgtcgg gctctttcca ctggttctgc tgacgcgtcc 120360
aggccagtcc cgagaccgtg agacgcgcct gccacatctg cttgcccgac gcgtgaatca 120420
cagcgtcagc tacgggcagg tgtcggtgtt tgcgctcggc cgccgacggg tagtggtgca 120480
cgttgatgct ggggatgttc agcatcttga gcggcagcgc gtacacatag atcgacatgg 120540
gctcctggct ggggcagatg cttcggcccg tggggttgtg cacgttgacc gacacgttct 120600
ccacctcgct gcccgtaaag tacgtgtgct gcacctgcag ctgattgtcg ccgcggtggc 120660
atggcgtcga gtcgggcgtg tactgcgaca ccaggatcag cgagggctgg ctcacgcgta 120720
cgtggatacc cgtctgcagg agtcgcgtct cgtgcggcag caccggcgtg tcgccgcgac 120780
taaacacggc tttcagcacg tgccccgaaa tgggacccag tacggatatc atttcgggac 120840
aacggcgacc gcgcgactcc atgctgcctg cgcgtacggg tgtaggcgac tgagcggcgc 120900
gccctttgcg gccgccgcct tacataggca ggcgaccaaa cgcggaaccc gaaataaaaa 120960
cgttctacac agagacaacc gcggattatt gagtgtcttt ttttattaca aaaaaaaaga 121020
ggcaaagccc caccgtcacc acaccccatc acacaccacc accgattttt tttgttttaa 121080
ccccgtatcg cgcggacgcc tagtgtccgt ttcccatcac cagggtcctc tgtttagaga 121140
tcgccgcaga ccatggctaa agtgacagga ctcgttttct ctgtcgtatt ttccgtgagc 121200
ttacagtctt gcggttccgt ctccggggac gccagtcgca tgggcagcag gtcctccagc 121260
gcgatggaag cgcccagcac cgagagctgc tgttgcgacg gcgaatggga cgtggaccgc 121320
gagtgtagcg tggatttgac ttggtgcgtc attgctgaca ggcaaccgcg attcagcgta 121380
tgctttgacg agataaaata gaggcgtccc aggagcgcgt cccgtgggaa cgtggcgccg 121440
ttctcgtcgc tcaccagtac ggttaattcc aaccaggagc gcggtagcca gaccgtaacg 121500
ggcattttga gtccctgacg gttgtgtggt acaaaaacac ccagataagg cccgtaaaag 121560
cggcggtaga tacgtaacgt gtgcgagttt ttcagcgtca attcgtaagg gacgcgcacc 121620
tccagtccct cgtccgccgc accggagcgt ggcggtacaa agtaaggcag tggcgcgtcc 121680
gaaaagaagg gtcgtcgcac cgtttcgcgt cgcagccgca ggcgaaacgc cactgggtcg 121740
gctggcgcct cggtgcggtc gcaggtcacg ttgaaacgta acatgccgtc ttggtatagc 121800
gtgagtgacg acagcgtcag gtccggcggt gattcgttcg ggtctagctc caatcgtcca 121860
aagacggagg gtcccaatgt cttggcagtg gtttccgaga ggcgcgccga gatacggctg 121920
gtgagtccac gcggccccga gatgccgcct tccactcgat gccagcacag cgcgtgtcgt 121980
acgcgcaccg tcagcgtggg cgtcagatcc gcgtccgttg attccgcggt atcagcgacg 122040
gaagccgcgt tctccgttac gttgtttata tccagcgtcg gctcgaacgt gagttctggc 122100
agatgcagcg caagacagtc gtgtaacgcc gtgtgatgcg cggctttacg tcgtagcggt 122160
agccgtttca gcagcggcgt gatgatacgg agcgcgaaga gattgagtga taagcgcacg 122220
atggccatgc gcgtcagttg ctggtcgatt actgagcgca ggatatggca gcctgggcgt 122280
gcgggaaaga gagagaaggc cgggcgtacg tcagaatcct cgttagatac cacgcataga 122340
atgccgcgtt cacgatcgtc gttgcggtca tcctcgtcct cttctttttt cttctctttt 122400
tccttttttt tctcgggctc atgggaagcc gccgtttctt cttcttgcga cgtcgcggag 122460
tcggtttgag actcgccgtt cgcttccccc aattgcagcg gcgtagagag cagaatctgg 122520
aagggatccc gcaattcttc gggtcggagg tcgaggtgca actggatcag atggtaggtg 122580
ccgcggtgca cccgaggctg acggatgtcg tgtttatccg tcagtgtgag gatggtctgc 122640
ggcgagccgc tgtgcttgtc cagctcgtcc ggcgttttca ggaggaggct gtcgtcgtcg 122700
gtactggcga cgcccatcat ggtcgtggtg gtagtggtgg cgaggaaagt gagcggcggc 122760
gctgacagag ctcggcgttg gcggcggcat ttgccgctgt gtcggctgct attgctgcca 122820
acgccaccgc cgccgcctcg tctggctcgt ggccggcggg cccgattccg aaggttgggg 122880
tcgacgcgtg gcatgcttgg tgtctgcggg cgcgagaggg ctggctcagc ctttaaatat 122940
gcaggtcgcg gatttgttat cgggtgaaac gtcacacacc gtgaagacga cctgttcgcg 123000
gatgaggtca tccagctgtc gcagcatgac gaaaagcgcc gacagccgcg cgatctcgtc 123060
gtcgggcgac acgtgctgcg gccgcgcggg cgtgcgcggc tcgccgacgc tgcgctcgcg 123120
gtccagccgc atcagcagct cctggcactt gacgagcagc atggagctgt cctctagcgc 123180
caacttgcgc acgtaggtca tggtcagctc cgaggctagg ttggccacca tggacatgga 123240
gaggcaggcg gtcttcatgt cgatcagcag gtgctggtcg atgaccggat cggggatggt 123300
gaaggtggcg tcgcgaaaag taatggtctg cagctgctgc acggcagcct ttacctcctc 123360
gtacgaacgg tcgagcgaga agaggcccat gatgagtagt cgctggttga tttccagcgc 123420
cagtggcatg ggtacgatcc agggcagcac cagctcccac tggcccagcg tcagcaggtt 123480
ctcgcgcgcc agcggtccgt ggaagagcgg cggcagcacg catagcgcgt cgcccttctc 123540
ccaagtcacg ggtcccgtgt tgaggacggt gtagagcagt ccgtgcgtcg gtacgtgtag 123600
gaggatctgg ttgccttcta cgcgccgcat caacgtcagc gtcatattgc gcagcaggcc 123660
gcgcagtcgt acgtagccgc gggtgtgatc tacgaactgg tgtaggccca gctggtagtg 123720
tttgatgaga tgtagacgct gcggaatggg cacaacggcc gctactagct tggtcagttt 123780
gcctacgtcg gcgatgctga gcttgtggtc gaaagtgcag aagatgttgg cctccatggc 123840
cgccatagcg gcggtgaaat cctggccgcg acggaggaga agcagagacg aacaacgtct 123900
gcaccgggcg cggcgtcaga gcgagcgtgg cgcgtccggg cccgcgtttg cgtctaggtg 123960
attcgccgtt aacctgcggt cgtcgccgtc ctcctcaccg gacggcctca cgagttaaat 124020
aacatggatt gctgtagcgg gatgatttcg cctacgacgt agttaccaaa gtgcgtttcg 124080
gacgtggcaa aagccccggc gccacccttg agtttggtct ccatcagcgc cagcgtggtg 124140
gtgctgagga tcggtagcgc ttcctgcgtc agacggcacg ggttttcgat gagttgttcc 124200
gtgccttcga cgcagacgta ctgcgtgtcc gtgtcgccgc ggatgcagtc cttggcgcgt 124260
agcaggtact cgtcgatggt tttgaagagc gttttgttgg ccgcgataat ctcttccgtg 124320
ttaaagtact gcgcgcaggg gctgtagaat ttggagttgt agcctagacg ttcgcgatgt 124380
cgggtgttgt agagtacgtc gctcagacag ccggcttgcg aggcccaggg gttgtgtgtg 124440
gccgcgaaag tctgtgcgtc cgcttcgcga tggtcgtaga tggccttggt ggcggcctcc 124500
gtgtcgtacg gatcgacggc cagcatgcag gaggcacgcc cgcgcgggtt gttggggatc 124560
ttaaagtaat taacgtccat cgtcaccggc gtaaggatta gttcgcacgc ggccttttgt 124620
ccgtgcaccg tggcggcggc attgcgctcg gacatgctgc cgaacgtcag catggagatg 124680
gtctccgtgt ctaacagttg cggccgttct acgccggccg cgtgccggat ccagcggtcc 124740
acctcgtcgt gccggtacac gttcataggg aagacgcgaa agaggtcctg cacgcggacg 124800
cccatgtcgg ttcgcacgcg gtttacgtag gctacgcagg tatttgacgt gtaacccaga 124860
cccatgtcta cggtgttaat gttctgcgtg acgtggtacg tggtgctgat gtcgcgttcc 124920
tccttggtca cgatagggtt gttgatgata actgacgtgc acgatttgcc gctgtagagc 124980
agcatgtcca cctcgaaggt gtcggtgcgt acggccgtga gtgcgaatcc cgggtggatg 125040
tgcgccttgg tctgcagcac cagtgaaact ggtgagattt tgtataacat ggcggccagc 125100
gtcatgactg agtgcaacac gttgggacag gtggccgagt aacgcgaaaa gggcgagcgc 125160
agccagttgt ggtactcgtg tgcgaaggct gtgggtagcg ggaaaccacc gtcgtgacgg 125220
tgatagtgcg ggaactcggt cacgtagcgt ttaatgtcgt cgctcaacgc cgcgcagatg 125280
gtggggtttg agtagaaacg gtggaaaggt acgggtaggc tgtactcgat caacgtctta 125340
ggcgccgtca cgacgcagca gccgttgtaa agcacgtgct gacgtgagat aaagtccggc 125400
aggccctgac gttgcgcgtg gtccagaggc gcgcgcactt cgagcacctt gacgtgctcg 125460
cccacgaatt gcacggccaa aaacagttca cgacaggcct gcagcagcgg cgtgtgcgcg 125520
tcggtggcga cgtcctccac cagctcggtc agcatctcgc ctacggcttg acgttgcgcc 125580
gctatcgagt cttcgggggt gacgccgctt gtgctctctt tcgacgtcgt acctgacgtg 125640
gagaccgcgg tggcggccgg catcaggaga aacgccggtc ggtaaaagag gtctactagc 125700
agcgtcttga ggttgagtcc caggccgcag gcccggttgt tggtcatggc gggcatgagg 125760
cagagataaa agaccttttg taacgtccat tcgtcgtcgg tggcacggta atcgtccaca 125820
aacagcggct cgtcggcatc catggcgccc aaacgcggta cgtcagaaac gccgtggtgt 125880
cgcgcctcga tgttggccgg gttcaacggt tgccggtcgg ccactacctg tacgccttcc 125940
atgttacgcg gcaggtgcgt aacgaagggg ggccacagcc ggtggtcgtg cagcgcgttc 126000
acgtaagccg atagcggttc ctcagccagt tgaccgttgt taagtcccgg cagcgctgag 126060
atgcgcgtta ccagacgcag cacggcgacc agattgcggt agtgaaagag caactgcggt 126120
ggtagggcgc catcagccag gtgttcggcg atcaacgtca ccagcgcgta gctgtgcgca 126180
aaaaccagca gctgacgtgt gtgaaacatg ttgacgatac aacgtgctac gaaagtgcgg 126240
attagcaaaa aagcgtcgac gttgccgtgt accagcacgt cgaccaggta gcagagctcg 126300
gggtaattgg ggcttgtcac ggtggttttg aaaagtcgca acgtctcttc gtagtcgggt 126360
ggtggccgca gtcgcatgtg ttccatgatc tcccaggtgc gcagttcgtg gaaggggccc 126420
ggtgccagtc catctggcaa attaccgatg acgatacgcg gtgtacacag cgccaccgtt 126480
tcgctgtttt cctggcagtg cgtaaagtcg aagaaggggt gcagctcggt gtagagcgtg 126540
atgttgccca ccttgtagaa gtcggtgacc acaaagtcct gcttcatttc gttcaccgtg 126600
cgcgggacct cgcgtcgtac gcggtaaaaa tgcggtatgc ggcgcgccgc accgcccatg 126660
ggttcctgct gaaaacgaca ctcgagcagt cgttgcatgg cgggttccga gggcggtccg 126720
cgttccgtga aggtctgtag acagggcgcg ggctcgtgca gcaccgggtg gcacagcgtc 126780
ttgagcgcgt ccacaaagtc tatcttttgt acggcacggt cccggtttag caggtaggcc 126840
gtggtgggca acgcgttgcg aacggtgtcg ttaagcttaa ctttgctttc caccgtggtg 126900
taaccgcgat cctcgggcag atacagccct acggggaaga aaaacgtcag gtccacgtta 126960
cgttctagcg gatctttggt atcggtgttt ttgtagacgc gccgcaagtt ttccataatc 127020
accgtttttt cgcccagtcg gatcacgtcc atgctcagcg gcgttaagct gtgcgccccg 127080
gcctgcgaaa gcgagtcgtt gggcaaatgc ggttggcccg aagtcagatg agccttgtac 127140
gagttgaaat cggccaggat cgagtgatag gatatggcag tgacggcatt ttcgggactg 127200
agtacaaaat tgccgtaggt ggccggcgcc gagaccgttt ctttggtgat gtggcttgag 127260
agcagcgaca tgatgatctg cataacgttg gccgtgctta ccatcacgcc gctgatcttg 127320
gcccccgagc tcgtggtgta cgtcgtgggg ttgtctagga tgctatcggt ggccgcttcg 127380
gctagacgcg tgaggaactt gagcacatag tcgcgatcgc gcgtgcgatt cagcaaaaag 127440
agcgtggcca gcattttggc cttgaagctc tgcaagatgt tgcttcgctg gatgcggttc 127500
agcgcctgtc gcgccagcgt ggcgttctct accagcgtct gcactacaaa gtacggcggc 127560
gccttgcgta gcagtgtctg taaaaagctg tgaatcaagc cgcgttccat ggcgtcggcc 127620
gtgtttttga gcgcgcgcag caccgtgtgc atagcttcca cgttgaggat cttgtccagg 127680
atggtgcctt cgaacgtctc gcgcagatac gtgaggcagg ctgcgctgag ctcaaagggg 127740
atggtgatgg gggatttttc actgtatttg gtgaccataa tggtggtctg acgactggtg 127800
ggcaaaccgg cgccgctggc cacacgcggc acctgcacgt ggaacagcat ttttcccgta 127860
gtcagtttat tgaggtcgtg gaacttgatg gcgtgcgccg ccgcggccaa gccgctggtc 127920
aaaaaataaa cccattccag gcgattgcag aaggtgccga agatggcttc gaagtgaata 127980
ttgtaacgct cggggtcgtc gccgtagtag atgcgtaagg cctcgaacat ctcctcgccg 128040
gcgctggtct tgacgtgcgt cagaaagtca gtgggaatgc ctactttagg caggagctcg 128100
agcgccgacc agttctccat cgcggcggcg gcgtgagcgc gaggcgtcgg agctcgggga 128160
aagcagcgcg acccggagaa tggccggcgc tgcgccgcgc cgcctcggct gtgacgctct 128220
aatagtcgtc ggcggctccg ctacgccgcg ccgggtttta cacgtccccg tgcacgttcg 128280
cgcctgcaac ctcacccaag agctatcgac gggcgaggac gcccgcttct gtcgtccgcg 128340
acccgttaac gtcgaacggg tgcgcgctgt ttttgcggct ctctaccgtg cctgtccgat 128400
acacgtgagg accgagcccg agcgtgtcaa gctggtactg ggtcgtctgt tactgggacc 128460
cgtggccgta ccctgttttt gcgacggtga agtggagggc cacggtgaac atctggtacc 128520
tacgacgcag ttttgtcgcg ggccgctgct ctacgtgcac cgacgttgtt gttgcggatc 128580
cgtgaccgcc gggcgcgcgc tgtcctacca cgttctcgaa aaccacgtgg ccacgcatgt 128640
gctacgcgga ttgctctcgc tgacggaatg gaatcgagaa ttgccgagcc tcttttgcga 128700
ctgtcctggc ggcggtggcg cctcgggaac cgaggaacgc tacgccatgg cctgcctgcc 128760
gcgcgacctc agcctgcacc tggacgacta tccttacctg atggtggaaa tcggacgcgt 128820
actcagtgtc agcgaggtag acgactacgt aaccgccgtc tccggctacc tgggcgaggc 128880
cgcggcgccg cgcattcagg ttcactacaa gctgctcttt ggactcaacg tgcgtccgca 128940
agcgccgtgc gcgttggacg ctacacgcga cttttttctg ctggagctgc aaaagctttg 129000
gctgggcgtt gaatatcacc acgaagtgac gtcggagttt ttcggtcgcg tgctggccca 129060
gctgcatcgc gaccgcgccc gcgtcatgat ggcgcttcgc ttgcccgagc agacggtgtg 129120
ccacctgagc accttcgttc tcagtcgctt caagcgacag gtactgtact tcaagttaca 129180
ggtgagctac ggcaagtgcc ggactggcca cgctgacaga agtgggggag gggggaacgg 129240
tggaaatcag ggacaccaca acctactgtg ttatcgacgc cttagcgtca catttgccga 129300
cacagacacg gtgtggagaa accttttcta cgtttattac gaactagctc gggatctggg 129360
gtcccatggg acagagaacc gacccgtaaa ccgcggttac ggtgtttctt gcgctccgag 129420
gacgtcgcgg ctatcaccgt cagaatcgac ggtggtttcg gcgaacggac acgcgctgtc 129480
ttccaccgcg ctcccgacga cgagcgcggg tcacaagctg tcactgccgc gcgacccggc 129540
cgccgatcgc gttcgacgtt acgtgtgcat tatctcgcgt ctcatgtacg ctcggtacgg 129600
ggagagatgg cgtaaacacc gtcaacggcg gtcggagacg ggagaagagg aggaggaaga 129660
gacgctggaa tcgggggaga ctgacgccac gccgccattt gactttacgg ggcagcagct 129720
gcgccgggcc tatcaggaac accgacgtcg taaacatcta gccgtgcagc gttacgcgcc 129780
gtgccgtcgt aagctcatcg gcgggatgga gtttgccgag gtgacgggcg tgagtctgga 129840
ccgcatcgcc gtcaacgctt tcaacaccaa ccgcgttatc aatatgaagg ccgcgctctc 129900
gtccatcgcc gcgtcgggtc tcggcgtgcg cgcgccgcgg cttcccaaga acatgaccca 129960
cagttttgtg atgtacaagc acacctttaa ggagcccgct tgcaccgtca gcacctttgt 130020
ttccaacgac gccgtctaca tcaactcgct caacgtcaat attcgcggtt cctaccccga 130080
gtttctgtac tcgctgggcg tgtaccggct gcacgttaat atcgatcact tttttctgcc 130140
ggccgtggtg tgcaatagca actcctcgct ggacgtgcat ggactggagg accaggcggt 130200
gatccgctcg gagcgcagca aggtgtactg gaccaccaac tttccgtgca tgatctcgca 130260
tactaacaac gtcaacgtgg gctggttcaa agcggctacg gccattgtgc cgcgcgtctc 130320
gggcgctgac ctggaagcca ttctgctcaa agaactctcg tgcatcaaga acatgcgcga 130380
cgtgtgcatc gattacggtc tgcaccgcgt tttcacgcaa ctagagctgc gcaattcgta 130440
ccagatcccc ttcctggcca agcagttagt gctgtttctg cgtgcttgcc tgctcaagct 130500
gcacggtcga gagaagcggc tgcagttgga ccgcctagta tttgaggcgg cacagcgggg 130560
tctctttgac tacagcaaga acctcacggc gcacaccaag atcaagcaca cttgtgcgct 130620
catcggcagt cgtctagcca acaacgtgcc caagatcctg gcccggaaca aaaaagtcaa 130680
attggatcac ctgggccgga acgccaacgt gctgacggtg tgtcggcacg tggaagccca 130740
caagatccct cgcacgcgcc tcaaagtgtt agtcgaggtg ctgggcgcgt tgcagagtat 130800
cagcggtacg ccgcacacgc gcgaagtgat ccaccagacg ttgtttcgat tgtgctcggc 130860
ggccgcagcc acatcgggcc tgtgttcatc ccctccccca ttgtgtgtgt cctcatcttc 130920
ctccgtcccc tctgtcccaa cctccgtcag cgttgacggc agttctgaac ccacgtcgcc 130980
gcgagcgcgg tttgcatcac gatgatggaa gccgcggccg ctgccgccgc ggcgtttcgt 131040
ccggaggagc gtccgacgcc gggttggcac gacgcggcgt tgttaatgga cgacggtacg 131100
gtgcgcgagc acgcgtttcg caacggaccg ctgtcgcaac tgattcgccg tgtgttaccg 131160
ccgccgcccg acgccgaaga cgacgtggtt tttgcatccg agctgtgttt ttattgcagc 131220
ggtcgtttta accgcaggtc gtccgtcttc tccatctatt ggcagaagca tagcgatctg 131280
gtgtacgcgc ttacgggcat tacccattgc gccaagttgg tggtggaatg cggtcagttg 131340
gggagtagta ggctacggtg gcgcgacggt gatgcgagtg gtgaggagcg ccggggagac 131400
gacgacagca gggacgagct gtacgacgtg ccgggcattt atatgattcg cgtcaacgac 131460
ggcggcagca ccggtcccag acacgttatt tggccgggta ccagcgtgct ttgggcgccg 131520
gacgttgtga tcactacggt gcagcgacga atctcggcgg cgcgcgccct ggtgaacacg 131580
ttccgccaat attttttttt gctggaacgg cgctcgcacg aggagctggt tctttgtccg 131640
cccgagatgg aggagcgtct agcgccgttg ttgcagagtg ccacgcgcgg tgattcggac 131700
atgtttgacg gtgtggtggc cagcgcttat caccgtttgc gaatgagtaa tattccgcgt 131760
tcatccgccc gtctgctgga gcactgcgtg gggctggcgg gtgctaagaa gctgctcttg 131820
ctcgacgtgc cgcgtctgga gaactatttt ctttgtcagg tctgtcttta cgagctggac 131880
gaggacgaga tgggcgagga gatgctgggc atgttggccg gaaagcccga ggatgccgcc 131940
gtctcgggcg caagcggcgg ttttctgcta catcgcaaga cgatgaagct ggccgcctgt 132000
ctgtgtttgt tgctcaattc gctgcatttg caccaggagg cgctggaggc cttggatcct 132060
ccgccgccgc gcgtcgagga gaacgacctt gtcaacgtgg tgctgcgccg ttattatcgc 132120
agtcacggcg gcgtgcaggc gcggacgctg gcggcggccc gggctttgtt agccgactac 132180
gctgaaacgt tttcgccttt ggggagtttt acgcgcctgg gttacgatcg tctcgtttct 132240
gccgatgccg gcgtcagtcg ccggcacctg gtggctctgc tgcgtgccta gctgaccctg 132300
aaacggatgg cgtgtatatc gtcacacagg taggtggcca tgatgacggc gatgataaga 132360
tcgtccgaga tacgattctg gcgcttggcc gagtagcgtg ccgtcgtgcc ttcggccagc 132420
gtgacgcggt gcaggttctg aatctgctcc agaagatact cgatggggtc gtggctcagc 132480
ttgatggtgt aggagacgag ctcttgcgag gctttgatgt agcccgagtt gaaacgcgag 132540
atgaactgtt ccacggccag cgccttgtcg cggcccatga ggtagaaggg ctgttcgatg 132600
tggttctggt cgggcgtgtg gtagaagagc acgcggatga gcgtgctgct ctgcacgctc 132660
tgtcggatga ggcaggcgat gcgcacggcc gccgcctggt tggtgttgcc ctccacggcg 132720
atacgcagtt cgtccaggta agggtgcagg ctcagcaccg agatgatcat gtgcgccgcg 132780
cactcggcga tggctacctc agaactctcg gagaggtcgc gcaaaaagaa atgctctagg 132840
ccgtaaatga gaaactggtg tcggtaggcg cctacggccg ccacgcccgt gcccgaggcc 132900
ttgcggttgg tggtgaaggc cgggtccaga tacacgtaaa gcgtcttgcc gaaataatcg 132960
taggcgttgg tgttgagcgt gctgtaacgc aaaatatcga actcttcgcg gctctggtcc 133020
gtgatgagca cggtgttctg cgagatttta ttggtaccgc cgatgatctc gtccatgaaa 133080
gcgcccggca taaacatgtt ggccgtcttg cgcacctgcg agttgaggct gatgaaggtg 133140
ggcttgtgca gtcggtagca aggacacgcc gtggcgtcgc ccttctccgt gaagctgtgc 133200
aggtgctctt cgcacacgta agagaccacg ttgagcatgt caaagggcgc attgttgagg 133260
cgcgtcaaga aacacgtggc gtcactggta gtgttggtgg acgatatgaa gatgatcttg 133320
gtggtattct gggccaggaa ccccagaatg gtgttgaagg cctctttctt gatgaagtgc 133380
gcctcgtcca ccagcagcaa gtggaagttt tgtcctcgga tgctctgtgt agagaggaga 133440
cagaaaaggg actcttataa ttacgcacgc tcggctggaa gcctacagag tcggggtggg 133500
gccggacagg tgagccaggt gagccgccag gtgaggcggg atcaccgtgt gccaaccggg 133560
ctgcgacctg aaaaccggaa ccaatccgcc gacaccggcg ccgcgtgacg cgcgcccata 133620
aaaacgaaag tgtcgtcgtc gcgacccgcc acagccgcca tgaactcgtt gctggcggaa 133680
ctcaaccgac tgggggtcgc gcacgccact acggaggatg tttttatctt tgtcgaccgc 133740
ctctttcaac acttttcctt ccttttccag gccgaggagt caggcccgcg ccgcttggaa 133800
ctggtcgcgt ccgtgttcga gcacctgacg gtggagtgcg tcaacgacat cctggacgcc 133860
tgcagccatc cggacgtgaa cgtcgcggag acaagcaaca cctgtcgtcc ctgcccttct 133920
cctgccccct ccgcccccaa aactgtcagc gacgctcaga cgtcatgtgc gacgcctcgg 133980
gcgcctgtga catgaggcac gtccagaacg cgtttaccga ggagatccag ttacattcgc 134040
tctacgcgtg cacgcgctgc tttcgcacgc acctgtgtga tctgggcagc ggctgcgcgc 134100
tcgtctccac gctcgagggc tccgtctgcg tcaagacggg cctggtatac gaggctctct 134160
atccggtggc gcgtagccac ctgttggaac ccatggagga ggcctcactg gacgacgtca 134220
acatcatcag cgccgtgctc agcggcgtgt acagctacct catgacgcac gcaggccgtt 134280
acgccgacgt gatccaagag gtggtcgagc gcgaccgcct caaaaagcag gtggaggaca 134340
gtatttactt cacctttaat aaggttttcc gttctatgca taacgtcaac cgtatttcgg 134400
tgcccgtcat cagccaactt tttattcagc ttatcatcgg tatctactca aagcagacca 134460
agtacgacgc gtgtgtcatc aaggttagtc gtaagaagcg cgaggacgcg cttctgaaac 134520
agatgcgttc cgaatatgga aacgcacctg tattcggatc tggcgtttga agcgcggttc 134580
gctgacgatg agcaattgcc tctacatctg gtgctcgacc aggaggtgct gagtaacgag 134640
gaggccgaga cgctgcgcta cgtctactat cgtaatgtag acagcgctgg ccgatccgcg 134700
ggccgcgctc cgggcggaga tgaggacgac gcaccggcct ccgacgacgc cgaggacgcc 134760
gtgggcggcg atcgcgcttt tgatcgcgag cggcggactt ggcagcgggc ctgttttcgt 134820
gtattaccgc gcccactgga gttgcttgat tacctacgtc aaagcggtct cactgtgacg 134880
ttagagaaag agcagcgcgt gcgcatgttc tatgccgtct tcactacgtt gggtctgcgc 134940
tgccccgata atcggctctc aggcgcgcag acgctacacc tgagactggt ctggcccgac 135000
ggcagctatc gtgactggga gtttttagcg cgtgacctgt tacgagaaga aatggaagcg 135060
aataagcgcg accggcagca ccagttggcc acgaccacga atcaccgtcg gcggggcgga 135120
ctgcgtaata acttagacaa tgggtcggat cgccgtttgc ccgaagcggc tgtggcttct 135180
ctggagacgg ccgtcagtac tccatttttt gaaattccga acggagcagg aacctcctcc 135240
gcgaacggcg gcggcagatt cagtaacctg gagcagcggg tagcgcgttt gttgcgcggc 135300
gacgaggaat tcatctatca cgcgggtcca ttggagccgc cttccaagat acgcggtcat 135360
gagttggtgc agctgcgcct ggacgtaaat ccagacctca tgtacgccac cgatccgcac 135420
gaccgcgacg aggtcgcgcg tacggacgag tggaagggtg ccggtgtctc gcgtctccgc 135480
gaggtctggg atgtgcagca tcgcgtgcgc ctccgtgtgc tgtggtacgt caattccttt 135540
tggcgcagtc gcgagctgag ctacgatgac cacgaagtcg aactataccg ggcgttggac 135600
gcttatcggg cgcgcatcgc cgtcgagtac gtgctgattc gcgccgtgcg cgacgagatc 135660
tacgctgtac tacgacggga cagcggcgcg ttgccacagc gtttcgcctg ctacgtgcca 135720
cggaacatgt cctggcgcgt tgtttgggaa ctttgccgtc atgccttggc gctctggatg 135780
gatcgggcgg acgtgcgtag ctgtattatt aaggcgctaa cgcctcgtct gagccggggt 135840
gccgccgctg ccgctcagcg agctcgtcgc cagcgcgagc gctcggcgcc caaaccgcag 135900
gagctgcttt tcggaccgcg gaacgagagc ggtccgcccg ccgaacggac ttggtacgct 135960
gacgtggtgc gctgcgttcg cgcgcaagtg gatttgggcg tggaagtgcg cgcggcgcgt 136020
tgtcctcgca ccgggctttg gatcgtccgt gatcgtcgcg gacgcttgcg acgttggctc 136080
tcgcaggccg aggtgtgcgt gctctacgtc acgccagact tggactttta ctgggtgctg 136140
ccgggcggct ttgccgtctc ttcgcgcgtc actcttcatg gcttggcgca gcgggctttg 136200
cgagaccgat tccagaactt tgaagcagtt cttgcaagag gaatgcatgt ggaagctggt 136260
cggcaagagc cggaaacacc gcgagtatcg ggccgtcgct tgccgttcga cgatctttag 136320
tccggaggac gacggctcgt gtatcttgtg ccaattgctg ttgctctacc gcgacggcga 136380
atggatcctc tgtctttgct gcaacggccg ttatcaaggc cactatggcg tgggccacgt 136440
acatcggcgt cgtcgacgca tctgtcattt acctaccttg taccaactga gcttcggagg 136500
tcctttgggt ccagccagca ttgatttctt gccaagcttt agccaggtga ccagcagtat 136560
gacgtgcgat ggtattacgc ccgacgtgat ttacgaggtc tgcatgttgg tgccccagga 136620
tgaagccaag cgcatcctgg tcaagggtca cggtgccatg gacctgacct gtcagaaggc 136680
agtgacgcta ggcggcgccg gcgcctggtt gctgccgcgt cccgaaggct acacgctttt 136740
cttttacatt ctgtgctacg acctgtttac ctcatgcggc aatcggtgcg atatcccttc 136800
catgacgcgg ctcatggcgg cggccacggc ctgcgggcag gcgggttgca gcttttgcac 136860
ggatcacgag ggacatgtag atcccactgg caattacgtg ggttgcaccc ccgatatggg 136920
ccgctgtctt tgttacgtgc cctgtgggcc catgacgcag tcgctcatcc acaacgatga 136980
acccgcgact tttttctgtg agagcgatga cgccaagtac ctatgcgccg taggttctaa 137040
gaccgcggcg caggtcacac tgggagacgg cctggattat cacatcggtg tcaaggattc 137100
tgagggccga tggctgcccg tcaagaccga tgtgtgggac ctggtcaagg tagaggaacc 137160
tgtgtcacgt atgatagtgt gttcctgtcc ggtgcttaag aacctagtgc actaacgggg 137220
tctgacagtt cacggggaga agaaacaaga aataacaaaa aaaaaaagag gacatggact 137280
cgccacggtt tgtggcaagg cgtatgttat catcatggag ctactcacgt tggtgttgta 137340
gcaactggca aaaagcgccg tgctcttggc gccgcggtgg tcgatgctga tcacgttgtc 137400
cttgttctcg accacgtagt cgcgcgcgaa ggtgtggcgg cagcggaact cgacctcttt 137460
gagcacaaac tgcgacacgt gcttttggtg cgccacgtag ccgatgctga tgccgatcat 137520
gtgcttaagc agaaacgaga taatggggat gatgaaccaa gtcttgccgt gacgtcgcgg 137580
caccaggaac acggtggctt tctgcttaaa gatgtcgatg gaggtctgcg agaggaagtc 137640
gatctggaag gcgtggatga ggtactgcag cacgcgattg gccagcacgg ggatcttggt 137700
cacggctata aaaaagatga cgtgtatcaa taaattcttt tgaaacggtt cgagtcggat 137760
ggcttttgcg tcgccctcga cggcggtact gaagccgccg tcgagccact ttttaaagtc 137820
ggtcatgaag ttgttgatct gctgaaactg cggatcgcgg tagagctcgg tcaacgcgtc 137880
cagcttctgg taggaggcgc gctgctcctc ggagcacggg cgaaacgtca gttcatcgag 137940
cgcgctcttg aggcgctcgt gaaacagcag ctcgcgctgg ctttcctcgg gcgagttgta 138000
gtcgcggtgg cggccgcaga aggccatgag cggcaggaag gcctcgttgc acgagtgggc 138060
cagcccgagt tcggggtgca tcatctggta gcgcttgcgg cacagcgccg ccacattggt 138120
gaaggccgtg gagatgcagg aggtggggtg gctcttgcgc ttctgcagct ccgcgtagcg 138180
ctcctggatc ttggcggccg aatctccgcg caacatgatg gcggcggcgg tggtgcgagc 138240
ggaggttagg cggcagcggc gagaggagag gaaaaagatg gcgtccgcga ggacgacgga 138300
ggatccaccc gaaaaccacg ttgtcgcgga cgtggcttgt gggacgggcg ccgtcactcg 138360
ttcgtcttcg tcgtccttag tggtgtcgtc ctcctcggcg tcaggctcag acgaatcttc 138420
atccgcctct cctctcagtt tccccgtctc ctccccctca actgccgtca ggtctccggg 138480
gtccgccggg gtttcaacgt ccctgtgctc ggtggaacgg atggtcgagc tgtcggcgca 138540
gtctccggcc gccgatttct cggtctccga ggcttggcgc ttcgaggagg ccgtaaatat 138600
ggcgctggtg gcctgcgagg ccgtgtcacc ttacgatcgc tttcgcctaa ttgaaacgcc 138660
cgacgagaat ttcttgttgg tcactaacgt aattccgcgc gagtcggccg aggtgccggt 138720
gttggatagc agtagcagcg gtggcgatag cgggccggag gacaaaaaga aaaacgtcgg 138780
gaataaaacc gcgggggaaa agaacggcgg tgggtctcgg gccaaacgcc gtcgtagacg 138840
acgcgctccg aaaaacgacg ccgccacgcc gtcttttcta cgtcgacacg acgtgctgga 138900
gcgtttcgcg gccgcggctg agcctttgcc gtcgctttgt gtgcgtgatt atgtgttacg 138960
caatgctgac cgtgttacct acgacggcga attaatctac ggcagttacc tgttgtatcg 139020
caaggctcac gtggagctgt cactctccag caacaaggtg caacacgtgg aagccgtgct 139080
gcgacaggtg tacacgccgg gcttgttaga tcatcacaac gtgtgcgacg tggaggccct 139140
gctgtggctg ctgtactgtg gaccgcgaag cttttgcgcg cgtgacacct gtttcggtcg 139200
cgaaaagaac ggctgtcctt tccccgcgtt gttgcccaaa ctcttttacg aacccgtgcg 139260
ggactatatg acctacatga atctggctga gctgtacgtc tttgtttggt atcgcggcta 139320
cgaattccct gcgccgacgc cgcaggcgac gacggcgggt ggtggtggta gtggtggcgg 139380
cggcggggcc ggcgcttgtg cggtcgagac gagcgcgtca gcaggccggg tcgatgacgc 139440
cggcgacgag gtgcatttgc ctttaaagcc cgtctcgctg gaccgtctca gagaggtatt 139500
gcaggcggtg cgcggccgct tctcggggcg cgaggtgccc gcctggccgg cctcgtcgcg 139560
cacctgtttg ttgtgcgcgc tctacagtca gaaccgtctc tgtttagatc tcgcgcgtga 139620
cgaggcgcgg accgtgagtt atagccccat cgttatccaa gactgcgccg cggctgtcac 139680
cgacgtcact ttgagccaca tcttgcccgg ccagagcacc gtctcgcttt tccccgtcta 139740
ccacgtcggc aagttgctgg acgctctctc gctgaacgac gcgggtctca tcacgttgaa 139800
tctatgacgt cggtcaacaa acagctctta aaggacgtga tgcgcgtcga ccttgagcga 139860
cagcagcatc agtttctgcg gcgtacctac ggaccgcagc accggctcac cacgcagcag 139920
gctttgacgg tgatgcgtgt ggccgctcgg gaacagaccc gatacagtca gcgaacgacg 139980
cagtgcgtgg ccgcacacct gttggagcaa cgggcggccg tgcagcaaga gttgcaacgc 140040
gcccgacagc tgcaatccgg taacgtggac gacgcgctgg actctttaac cgagctgaag 140100
gacacggtag acgacgtgag agccaccttg gtggactcgg tttcggcgac gtgcgatttg 140160
gacctggagg tcgacgacgc cgtctaacag gtatagcaat ccccgtcacg cctctgttca 140220
tattttatta aaaaaaaaca caacataacg acagtgtcgg tgtggtagct agtgcagccc 140280
taggaacagg gaagactgtc gccactatgt cctccgcact tcggtctcgg gctcgctcgg 140340
cctcgctcgg aacgacgact cagggctggg atccgccgcc attgcgtcgt cccagcaggg 140400
cgcgccggcg ccagtggatg cgcgaagctg cgcaggccgc cgctcaagcc gcggtgcagg 140460
ccgcgcaggc cgccgccgct caggtcgccc aggctcacgt tgatgaaaac gaggtcgtgg 140520
atctgatggc cgacgaggcc ggcggcggcg tcaccacttt gaccaccctg agttccgtca 140580
gcacaaccac cgtgcttgga cacgcgactt tttccgcatg cgttcgaagt gacgtgatgc 140640
gtgacggaga aaaagaggac gcggcttcgg acaaggagaa cctgcgtcgg cccgtagtgc 140700
cgtccacgtc gtctcgcggc agcgccgcca gcggcgacgg ttaccacggc ttgcgctgcc 140760
gcgaaacttc ggccatgtgg tcgttcgagt acgatcgcga cggcgacgtg accagcgtac 140820
gccgcgctct cttcaccggc ggcagcgacc cctcggacag cgtgagcggc gtccgcggtg 140880
gacgcaaacg cccgttgcgt ccgccgttgg tgtcgctggc ccgcaccccg ctgtgccgac 140940
gtcgtgtggg cggtgtggac gcggtgctcg aagaaaacga cgtggagctg cgcgcggaaa 141000
gtcaggacag cgccgtggca tcgggcccgg gccgcattcc gcagccgctc agcggtagtt 141060
ccggggagga atccgccacg gcggtggagg ccgactccac gtcacacgac gacgtgcatt 141120
gcacctgttc caacgaccag atcatcacca cgtccatccg cggccttacg tgcgacccgc 141180
gtatgttctt gcgccttacg catcccgagc tctgcgagct ctctatctcc tacctgctgg 141240
tctacgtgcc caaagaggac gatttttgcc acaagatttg ttatgccgtg gacatgagcg 141300
acgagagcta ccgcctgggc cagggctcct tcggcgaggt ctggccgctc gatcgctatc 141360
gcgtggtcaa ggtggcgcgt aagcacagcg agacggtgct cacggtctgg atgtcgggcc 141420
tgatccgcac gcgcgccgct ggcgagcaac agcagccgcc gtcgctggtg ggcacgggcg 141480
tgcaccgcgg tctgctcacg gccacgggct gctgtctgct gcacaacgtc acggtacatc 141540
gacgtttcca cacagacatg tttcatcacg accagtggaa gctggcgtgc atcgacagct 141600
accgacgtgc cttttgcacg ttggccgacg ctatcaaatt tctcaatcac cagtgtcgtg 141660
tatgccactt tgacattaca cccatgaacg tgctcatcga cgtgaacccg cacaacccca 141720
gcgagatcgt gcgcgccgcg ctgtgcgatt acagcctcag cgagccctat ccggattaca 141780
acgagcgctg tgtggccgtc tttcaggaga cgggtacggc gcgccgcatc cccaactgct 141840
cgcaccgtct gcgcgaatgt taccaccctg ctttccgacc catgccgctg cagaagctgc 141900
tcatctgcga cccgcacgcg cgtttccccg tagccggcct acggcgttat tgcatgtcgg 141960
agctgtcggc gctgggtaac gtgctgggct tttgcctcat gcggctgttg gaccggcgcg 142020
gtctggacga ggtgcgcatg ggcacggagg cgttgctctt taagcacgcc ggcgcggcct 142080
gccgcgcgtt ggagaacggt aagctcacgc actgctccga cgcctgtctg ctcattctgg 142140
cggcgcaaat gagctacggc gcctgtctcc tgggcgagca tggcgccgcg ctggtgtcgc 142200
acacgctgcg ctttgtggag gccaagatgt cctcgtgtcg cgtacgcgcc tttcgccgct 142260
tctaccacga atgctcgcag accatgctgc acgaatacgt cagaaagaac gtggagcgtc 142320
tgttggccac gagcgacggg ctgtatttat ataacgcctt tcggcgcacc accagcataa 142380
tctgcgagga ggaccttgac ggtgactgcc gccaactgtt ccccgagtaa ccgggacgcg 142440
gaacgtgacg gttgctgagg ggaaaggcaa cagagaaggt acaaacccac cggcggggaa 142500
aataccgagg cgccgccatc atcatgtggg gcgtctcgag tttggactac gacgacgatg 142560
aggagctcac ccggctgctg gcggtttggg acgatgagcc cctcagtctc tttctcatga 142620
acaccttttt gctgcaccag gagggcttcc gtaatctgcc ctttacggtg ctgcgtttgt 142680
cttacgccta ccgcatcttc gccaagatgc tgcgggccca cggtacgcca gtagccgagg 142740
actttatgac gcgcgtggcc gcgctggctc gcgacgaggg tctgcgcgac attttgggtc 142800
agcggcacgc cgccgaagcc tcgcgcgccg agatcgccga ggccctggag cgcgtggccg 142860
agcggtgcga cgaccggcac ggcggctcgg acgactacgt gtggcttagc cggttgctgg 142920
atttggcgcc caactatcgg caggtcgagc tcttccagtt gctggaaaag gaatcgcgcg 142980
gacagtcgcg caactcggtg tggcatctgt tgcgtatgga cacggtctcg gccaccaagt 143040
tctacgaggc cttcgtcagc ggctgtctgc ccggcgccgc ggcggcggac ggttcgggtg 143100
gcggcggctc gcactacacg ggctcgcgcg ccggcgtctc gccgggcatc cagttcggta 143160
tcaaacacga gggcttagtc aaaacgctgg tggaatgtta cgtgatgcac ggacgcgagc 143220
cggtgcgcga cggcctcggt ctgctcatcg accccacgtc ggggctgctg ggcgcttcca 143280
tggacctgtg cttcggcgtg ctcaagcagg gcagcggtcg caccttgctg gtggaaccgt 143340
gcgcgcgcgt ctacgagatc aagtgccgct acaaatattt gcgcaaaaag gaggacccct 143400
ttgtgcagaa cgtgctgcgg aggcacgacg cggcggccgt ggcctcgctg ttgcagtcac 143460
acccggtgcc gggcgtggag tttcgcggtg aacgcgagac cccgtcggca cgcgagtttc 143520
tgctttcgca cgacgcggcg ctcttcaggg ccacgctcaa gcgcgcgcgc ccgctcaagc 143580
cgcccgaacc gctgcgcgag tacctggccg atctgctgta tctcaataag gccgagtgtt 143640
cggaagtgat tgtgtttgac gccaagcacc tgaatgacga caacagcgac ggggacgcca 143700
cgaccactat taacgcgagt ctcgacctag ccgcgggcga cgccgctggc ggcggcgctg 143760
atcaccacct gcggggcagc ccgggcgatt cgccgccgcc gatacctttc gaggacgaaa 143820
acacgcccga gctgctgggc cggctcaacg tgtacgaggt agcgcgcttt tcactgccgg 143880
cttttgtcaa tccgcgtcac cagtattact ttcagatgct cattcagcag tacgtgctca 143940
gccaatacta tataaagaag catccggacc cggagcggat cgatttccgt gacctgccta 144000
ccgtctacct ggtctcggcc atcttccgcg agcgcgagga aagcgaactg ggctgcgagt 144060
tgctggccgg cggtcgcgtt ttccactgcg accacattcc gctcctgctc atcgtcacgc 144120
ccgtggtctt tgaccctcag tttacgcgcc atgccgtctc taccgtgcta gaccgttgga 144180
gtcgcgacct gtcccgcaag acgaacctac cgatatgggt gccgaactct gcaaacgaat 144240
atgttgtgag ttcggtacca cgcccggtga gcccctgaaa gatgctctgg gtcgccaggt 144300
gtctctacgc tcctacgaca acatccctcc gacttcctcc tcggacgaag gggaggacga 144360
tgacgacggg gaggatgacg ataacgagga gcggcaacag aagctgcggc tctgcggtag 144420
tggctgcggg ggaaacgaca gtagtagcgg cagccaccgc gaggccgccc acgacagctc 144480
caagaaaaac gcggtgcgct cgacgtttcg cgaggacaag gctccgaaac cgagcaagcg 144540
gtcaaaaaag aaaaagaaac cctcaaaaca tcaccaccat cagcaaagct ccattatgca 144600
ggagacggac gacctagacg aagaggacac ctcaatttac ctgtccccgc ccccggtccc 144660
ccccgtccag gtggtggcta agcgactgcc gcggcccgac acacccagga ctccgcgcca 144720
aaagaagatt tcacaacgtc cacccacccc cgggacaaaa aagcccgccg cctccttgcc 144780
cttttaactc ataaactttc aggtctcgcg tacgattcgc gagtcgggaa tgggacaccc 144840
gtgggtgttt ctccgtgtgt atattatttt tttttgtgtg tgtgtgtgtt tgcgcccccg 144900
tgtgtctaat gtgctgtttg aaacacgtaa agtagctggt ggaagaacag ataaaccttt 144960
aataaaaaaa aagtatgtgc tcccgaccca cggtctgcgt gtctcttttt tatgtccatg 145020
tctccaagtc tggtgcgggt ggcggcgggg tcaagcgtcc tcgaagtctt catcatcgtc 145080
gtcgtcctct tgttcgcgga ggcgacggct ttccaagctg tcgtggtgac tgagcgcagc 145140
gacttcttcg ccggaggctg tggccagcgc ctggtacttg acactgccgc taccgcgtcc 145200
gcgaaagtag cggacggcgc gacacgtcgt aaacatggcc catatgaaaa agagcatgcc 145260
gaacgaccag ctgatgccgg tgcggtattc gttgctgagg aaggtatcgt actgcacgat 145320
ggggtagatg aggccgcaga gtccaaagaa ggcgcccagg tggtagccga attgcacctt 145380
gacgtattga aaaaagacgg cctcgatcag taaaaagtag atgatggaga tgatagcgta 145440
gaccacgaag acggctaaca ccatgtggcc tgtacgcacg aaaaagttgt ttccgaagcc 145500
gtagcacagg gccatggcta ccacggtggt gttgaaacca agcgctacct ccaccaggtt 145560
gacgatgagc gtgcggaact gcaccgtacc tttgagcttg gggtgcagac gcgagaagaa 145620
aaagagtgag cgtttgtagc tgcggtactg cgtgaccatg ctcacgttga aaatggtcag 145680
gcagaaaaag tgcacggcgg ccatgaaggc gatcatgctg ggcagccgaa atgacatggt 145740
cagtgtgaat agttggaacg tgtccatgct gagaatgaag aggaaggctg tgaggctgtc 145800
gcccatgtac gaaatgtcgc gtgtcgactg gtttaggctc atgcctttgt ccttgcgcat 145860
gctgatcttg atccagcata ccaggtagta gatggtcacg gctaaaaaga cgagctgcat 145920
gaacacggcg tagcacacca actgcaccga gtctaagaaa agcataggcg tgtgcaggtg 145980
cattacgttg taggccgaca tgttgagcct ttcaaagtcc acgacgtgat agtagacgca 146040
ggggtagccc aggtgcggaa aattgctcag cactagatgc acgctgacgt tgacaaaagt 146100
gagcaccatg aaaacgatag aagcgctcca tgtccgtgta ttcactttat ccacgtgcga 146160
gggggccatg gcgatagcgg cggcccgctc gctcgggagg cgatgggggc gcgccgatga 146220
cgacaggctc gcgggtcgtt aaatactacg atgggagccg ccgcggctca cgacgcggtt 146280
tgagcacgtc cgggcgatcg gtgaaaaaag accccgcggg ccttcgcgac tctcttctgt 146340
ccgaggatga ccgctcagcc gccgctgcac caccgccacc acccgtacac cctgttcggg 146400
accagctgtc atctcagctg gtacggcctt ctggaggcct cggtgcctat cgtacaatgt 146460
ctgtttttgg atctgggtgg cggccgtgcc gagccgcggc ttcacacgtt cgtggtgcgc 146520
ggtgaccgtc tgccgccggc tgaggtgcgt gctgtgcatc gtgccagcta cgccgcgctg 146580
gcctcggccg tgactacgga cgccgacgag cgccggcgcg gcctagagca gcgtagcgcc 146640
gtgttggcgc gcgtgttgct agaaggcagc gcgttaatcc gcgtgttggc gcgcaccttc 146700
acgccggtgc agattcagac ggacgctagc ggcgtggaga ttttggaggc cgcaccggca 146760
ctgggcgtgg aaaccgcagc gctatcgaac gcgcttagtc ttttccacgt agccaagcta 146820
gtggtcatcg gctcgtatcc cgaagtgcac gagccgcgtg tggtcacgca tgccgcggaa 146880
cgcgtctccg aagagtatgg cacccacgcg cacaaaaaat tgcgtcgcgg ttactacgcc 146940
tacgatttgg ccatgtcgtt tcgcgtcggc actcacaagt atgtgctgga gcgcgacgac 147000
gaggccgtcc tggcacgcct ctttgaggtg cgcgaggtgt gttttttgcg cacctgtctg 147060
cgtctggtca cgcctgtcgg tttcgtggcc gtggcagtga ccgacgagca gtgttgttta 147120
ttgctgcagt cggcctggac tcacctttac gacgtgcttt tccgtggttt cgctgggcag 147180
ccgccgttac gcgactacct ggggccggac ctttttgaga cgggcggcgc ccgttctttc 147240
ttttttcccg gtttcccacc cgtgcccgtc tacgcggtcc acggtctgca cacgttaatg 147300
cgcgagacgg cgttggacgc ggcggctgag gtgctctcgt ggtgcggcct gcccgacatc 147360
gtgggctcgg ccggcaagct ggaggtggaa ccctgcgcgc tctcgctcgg cgtgcccgag 147420
gatgagtggc aggtcttcgg caccgaggcc ggcggcggcg ccgtgcgtct caatgccacg 147480
gcttttcgcg agcgaccggc cggcggcgat cgtcgctggc tgttgccgcc gctgccgcgt 147540
gacgacggcg acggtgaaaa caacgtcgtg gaagtcagca gcagcaccgg cggtgcgcac 147600
ccgccgagcg acgacgctac tttcaccgtg cacgttcgcg acgccacgct acatcgagtg 147660
ctcatcgtgg atttggtcga gcgcgtgctg gccaagtgtg tacgcgcgcg cgacttcaat 147720
ccctacgtgc gttatagtca tcgactccac acttatgcgg tttgtgaaaa gtttattgaa 147780
aatctgcgtt ttcgctcgcg acgcgccttc tggcagatcc agagtctgct gggctacatc 147840
tccgagcacg ttacgtcagc ctgcgcttcg gccggccttt tgtgggttct gtcgcgtgga 147900
caccgcgagt tttatgtcta cgacggctat tcgggtcacg gacccgtctc ggccgaagtg 147960
tgcgtgcgga ctgtggtcga ctgttattgg cgcaaacttt ttggcggcga cgatccgggt 148020
cccacctgtc gtgttcaaga gagcgcgccc ggcgtgctgt tggtttgggg cgacgagcgg 148080
ttggtgggtc ccttcaactt cttctacggc aacggcggcg ccggtggtag tccgctccac 148140
ggggtggtgg gtggtttcgc ggcgggacat tgtggcggcg cttgttgcgc gggctgcgtc 148200
gtcactcacc gccattctag cggtggcggc ggcggtggtg gtggcgtggg cgacacggac 148260
cacgcgagtg gcggcggtct agatgccgct gccgggagtg gtcataacgg cggtagtgat 148320
cgggtttctc cctccacgcc gcccgcggcg ttaggtggct gttgctgcgc ggccggtggc 148380
gactggctct cggccgtggg tcatgttctg ggccggctgc cggcgctgtt acgggagcgc 148440
gtgagcgtgt ccgagctgga agccgtgtac cgcgagatcc tctttcgctt cgtggctcgc 148500
cgcaacgacg tggacttttg gttactgcgc ttccagcccg gtgaaaacga agtaaggccg 148560
cacgccgggg tgattgactg cgcgcccttc cacggcgtgt gggccgagca gggccagatc 148620
atcgtacagt cacgcgatac ggcgttagcg gccgatatcg gctacggcgt ctatgtggac 148680
aaggcctttg ccatgctcac ggcttgcgtg gaggtctggg cgcgagagtt attgtcgtcc 148740
tccaccgctt ccaccaccgc ttgttcttct tcttccgttc tctcttccgc cttgccgtcc 148800
gtcacttcgt cctcttcggg cacggcgacg gtgtctcctc cgtcttgttc ttcttcgtcg 148860
gcgacttggc tcgaggagcg cgacgagtgg gtgcgttcgc tggcggttga cgcgcaacac 148920
gctgctaagc gggtggcttc cgagggcctg cggtttttcc ggctcaacgc ttaacgagtc 148980
acgtagggga actacgtggg taagtgacgt ggatactagt aaaaaaagtg cgtcaaagtt 149040
ctcagcgtgt gacgtggata ctagtaaaag ggacgtcaaa gctcactacg tgttgcgtgt 149100
tttttttttt tctatgatat gcgtgtctag ttcgcttctc actcttcctc tccccgttcc 149160
cagcgcggtg gcagcttggg gggtgagggc aaattggggt agttggcgtt gagcacgtct 149220
agcaggccca ggcccacggg ccaaccgtcc acggtcttac gctcggtcag cttgaggcta 149280
aacgagtgtg cctcgtcttg accggtaagg cggaaaaaga agcgtgctac cagctgcagg 149340
caggtatgcc gcgtctgctg gaagagcacg aaggtagcgg gcacgtactg cacaatgtgc 149400
ggttcttttt cctcaaagag taggtagagc gcgctgcaga tcagccgccg ggcgctgtgg 149460
tgcagcagcc ggccgaagct ttcgcgcacg ttcactgcgt ccaggtactg gagcaggtcg 149520
tgcaggcact tgcgcgttaa gttgcaattt tccacgcatg aaataacggt acagagcgcg 149580
aagtgcagca ggttgtcggc cttgacgatg ccgcagcggt gtttgagccg cagatccgag 149640
agcctcacct gcgtgacgac gtcttcggtc tcgagcaaaa acacggcgga gtagcccaga 149700
aaggccgagg tgcacagcaa ctcgctgcgg tactcggcca tggaaaccag cagcccgtgc 149760
tccgtgtgca gccacagctt gtcgccgcgc accgtaaagt cgagcacttg cggctccatg 149820
atcatcacat tctgtctagt gaaatccgta tggacctcca gcacgccgcg gatcatcagg 149880
gcctccattt cgaaatcggc cgacacgctc tgggccgcgc cgctcctcgt ctgccgtgat 149940
caagcggcgc ggcgcggacc tttcaagcgt tcctgggccg ccgctcgagg cagttcccct 150000
ttctggcact ccgcccgccg cttcgcggct catttggcgc cggcgcgcct tctcgcggct 150060
gcaaatcagc tccacgtatc ggcaaaactt gctgtcgtcg taggcggcgg ccacgatctc 150120
gccgaaggag agctgcaggt aggcttcggg tacggggtcc agcgtgccta gcgccaggat 150180
gtgacacaga tagggcaggg tcacgcgctc taccgtgtaa ttggagtaga cgatggcctc 150240
ttcggcccct tgatgcgtga ccagacgccg taggcgaaag gtgcggaaat actcgttttc 150300
ccacaactgc gtgaggaagc gttctagcga ctcggtgcca ggcacgaact gcgagaagaa 150360
gctgttggcc accaggcggt tgtcttccac cgccagcgga cggaagggcg ccgcgtcgcg 150420
cgccttgcgc acggcctcca acacgggcag gtggtagagt tcggcgtcgc gcgcgcccag 150480
gctcatggag tcctcgcgcc gcgaggcgta gcgcgtgagc aggtcgcgca gctcgcgcac 150540
gcgattctcc caggtctggt tgagcgtgcg caggtcctgg atctcgtcca cctgcgactg 150600
gatctgctcc tccaggcact tgatgacctg cttcttaaac aggtcgcgga tgtcccgctc 150660
gggcgccgcc gggccgggtg gcggcggcag cagcccgacg tggcccgcgg gtcctcccac 150720
cacggcgccg ccgggtccca ccacgccggg tccacccgga ccacgcgcgg gtagtagacg 150780
gttttggtcc accagcgagg gggtcaggtc ctgcagaaag gactcgacgc tgtcctcgat 150840
gccgatgcgc gatttgctgt ccgagacgtt aagcaaaaac ttcataatgg actttttggc 150900
gtcgctgccc cggtcgtgct gctccatcat ctccaccagc ttcttgcagt tgagctcgtg 150960
gcggctggcg gtcaccactt tcacaggaaa ggtattgagc agctggcaga tcttttggtg 151020
gcggcagagc ccgtcgtagc gcagaatctc ctcgtgcagg tgtgccaccg gcgtggtgaa 151080
cagcagcttg tcgcgctcat aagccagcgg ttcggccgcc acgtacaagc ggatgtgctt 151140
gccgcgcagc tgcgcctcca gccgctccga gcgcaccttc ttgaagacgc gtacctcggg 151200
cgcgttggct acgcgcacgg cgcccaggcg ctcggccacc tgcagcagca gcgccaggtt 151260
agcctgcagc aggtcctgcg ccagcgggtg tgtctcggtg gcccgctgca cggccgcgcg 151320
tacaaattgc gcccgctcgg ccgcctcgct cggcttggtt ttcacgtcca gcagcggtac 151380
cagtcccacc gttacgcacc aatccacgta gagaccatag tcgtcgttat cggcgtactg 151440
atataaaatg tcgcggagcg cgcccagcac gcccgtttgc acgctctggc gcaacgaggc 151500
gctccacacc aacagatact gctccaggtc ctcttcgtcc agcgcgcggt agggaaacag 151560
cgccgcgtgt aacttccact cctcggccac gcgccgcacc gtgatggtgt caaagagcgt 151620
cttgcacact ccgtagagca gctgcttgcg cagcacgcac gggtcgcgca gcacctggtg 151680
catgctctgg ccgcgacacg tccccagaaa gccgtgcagc aaccgcagga agctcatcgt 151740
ctggcccgtg gggaaaatgt cgatgacggc ctcgtcatcc acgccgcggc ccacgcccaa 151800
gtacgacgac gccttgatcc tcaacctctc gtcggctgcc aagatcgaac ggatcgtcga 151860
caaggtcaag tctctctcgc gcgagcgctt tgcgcccgag gatttttcgt tccagtggtt 151920
tcgctccatc agtcgcgttg aacgaacgac agataacaac ccctctgccg caactaccgc 151980
cgcggcaacg acgaccgttc actcctccgc ctcctcttct gccgccgctg ccgcttcgtc 152040
cgaggccggc ggcacgcgcg taccctgcgt cgaccgttgg cccttctttc ccttccgcgc 152100
gctgctcgtc accggcacgg cgggcgccgg caagacttcc agcatccagg tgctggcggc 152160
caatctagat tgcgtgatca ccggtaccac ggtgatcgcc gcgcagaacc tcagcgcgat 152220
cctcaaccgc actcgctcgg cgcaggtcaa gaccatctac cgcgtcttcg gtttcgtcag 152280
caagcacgtg ccgctggctg atagcgccgt tagccacgag acgctggaac gctaccgcgt 152340
gtgcgagccg cacgaggaga ccaccatcca gcgcctgcag atcaacgatc tgctcgccta 152400
ctggccggtc atcgccgaca tcgtggacaa atgcttaaat atgtgggagc gcaaggccgc 152460
ttcggcctcc gccgcggccg cggccgccgc ctgcgaggac ctctcggagc tgtgcgagag 152520
caatatcatc gtcatcgacg agtgcggcct tatgctgcgc tacatgctgc aggtggtggt 152580
gtttttttac tacttttaca acgccctggg cgacacgcga ctttaccgcg aacgccgcgt 152640
gccctgcatc atctgcgtcg gttcgcccac gcagaccgag gcgctggaga gccgctacga 152700
ccactacacg caaaacaaga gcgtgcgcaa gggcgttgac gtgctctcgg cgctgattca 152760
gaacgaggtg ctcatcaact actgcgacat cgccgacaac tgggtcatgt ttattcacaa 152820
caagcgttgc accgacctgg actttggcga cctgctcaag tacatggagt tcggtatccc 152880
gctcaaggag gagcacgtgg cctacgtgga ccgcttcgtg cggccgccca gctccatccg 152940
caacccctcg tacgccgccg agatgacgcg gctttttctc tcgcacgtcg aggtgcaggc 153000
ttacttcaag cggctgcacg agcagatccg cctgagcgag cgccaccgtc tcttcgatct 153060
gcccgtctac tgcgtggtca acaaccgcgc gtaccaggag ctctgcgagc tggccgaccc 153120
gctgggcgac tcgccgcagc ccgtcgagct ctggttccgc cagaacttgg cgcgcatcat 153180
taactactcg cagtttgtcg accacaacct ctccagcgag atcaccaagg aggcgctgcg 153240
ccccgcggcc gacgtcgttg ccaccaacaa ctcctccgtc caggctcacg gagggggagg 153300
atctgtcatc gggagcaccg gcggcaacga cgagacggcg tttttccagg acgatgatac 153360
caccaccgcg cccgatagcc gtgagacgct gctcaccttg cgcattacct acatcaaggg 153420
cagttcggtg ggagtcaact ctaaggtgcg ggcctgtgtt atcggatacc agggcacggt 153480
cgaacgtttc gtggacatct tgcaaaagga cacgtttatc gaacgcacgc cctgcgagca 153540
ggcggcctac gcctactcgt tagtttcggg cctgctcttc tcggccatgt actacttcta 153600
cgtgtcgccc tacacgaccg aggagatgtt gcgtgagctg gcgcgcgttg agctgcccga 153660
cgtgagttcg ctctgcgccg ctgccgccgc cacggccgcc gctcccgctt ggagcggggg 153720
agagaatccg ataaataatc acgtcgacgc ggattcttct cagggcggcc agagcgtgcc 153780
ggtatctcaa cggatggaac atggccaaga ggaaacccac gacatcccct gcctgtccag 153840
ccaccatgac gactcggacg ccatcacgga cgccgaactc atggatcaca ccagtctgta 153900
cgcggatccc ttttttctca aatacgtcaa gccacctagc ctggcgctgc tttctttcga 153960
ggagacggtg cacatgtaca ctaccttccg cgacattttt ctcaagcgct accagctcat 154020
gcagcgtctc acgggcggtc gcttcgccac gttgccgctc gttacctaca atcgccgtaa 154080
cgtggtgttc aaggccaact gtcagatcag ctcgcaaacc ggctccttcg tgggcatgct 154140
ttcgcatgtg tcgccggcgc agacgtacac gctcgagggc tacaccagcg acaacgtgct 154200
cagtctgccc agtgaccgcc accgcatcca ccccgaggtg gtgcagcgcg gtctttcgcg 154260
gctggtacta cgcgatgcgc tcgggttcct ctttgtgctc gacgttaacg tctcgcgctt 154320
cgtcgagtcg gcgcagggca agagtctgca cgtgtgcacc accgtggact acggcctcac 154380
ttcgcgcacg gccatgacca tcgccaagag tcagggcctg tcgctcgaga aggtggccgt 154440
ggactttggg gaccatccca agaacctcaa gatgagccac atctacgtgg ccatgtcgcg 154500
agtcacggac cccgagcacc tcatgatgaa cgttaacccg ttgcgactgc cctatgagaa 154560
gaacaccgct atcaccccct atatctgtcg cgcgctcaaa gacaaacgca ccacgcttat 154620
tttttgacac aacaccgtgt aagcaaaacg tgactttatt gagcagggta aaaaccacgt 154680
acaagaacca cgttgtctat ccccccaaaa aaaaacacac cgtcagggaa cacatcgcct 154740
atagatagcg gcactttaca taaaaccacc gtacctgcat cacggtggct cgatacactg 154800
gaaattcaat aaaaaccacc gtgtccacgt tacggtactt gccgggtcag cgtccttctc 154860
ttgagatttc tgttcgcaaa cttatccgtt tccccggtcc gcggtgtctc ctcgcgaggc 154920
tgacagtcta cgagtggtat ctacaagaga aagaaacccg ggtgggagcg acgccgtcgc 154980
tgggtatcaa ccccgcggct gaccgtcgtc cggtaaagga acaacccgtc gtcgcaagcc 155040
gggttcgacc aagagaaaaa aacccgggtg cggggggaga cgggtcgtcc tttggtcgtt 155100
cgcggacggc gtacatgccg cgtgggtcag tcgacggcgt cgctccgtgc ggtcggtcat 155160
cattctgctt cacatatatg ggttgtttgt gtttttttta taatgaatac gcactcatcc 155220
tatccgtgac tgcgcgtgtg gcagagagga tgccttataa catgtatttt gaaaaattgc 155280
caacagctat aatttctctc atgtagcaga atagagacct tttgtcgtct ttttgtttgt 155340
cattacttgt tttccaggga attagagaga gggaaccgcg cctccggcgg cggtgcccgc 155400
ggaccccggc cccttctcgc gtgcgcggtg tgactggttg agcgaatgag cagctaggct 155460
tggtggtgct ccgcgtgcgg gggagaagac gattaacaac aaaaaataag tggaagtggc 155520
cggtgggtct ttgtccgcgt gcgcgcccat ccgtcgccgg gaccgagcag aaagtgatgt 155580
ggtggtacat tgattttttc cttgacagga aagaaaaaaa agagttttgt tttcctatgt 155640
gagaggagaa aggtatgtga ggagatgttc gatgatcgta tgttacagtt atgctgtaag 155700
gaagctttta tcgtgcgtcc tgtttttcat ttgatgtata tgacacaatt gaaacctatc 155760
gataggcgta tatcgaggat tcatcaattc ttagaatcgt cgtctttttg gctaattgga 155820
ctttgcccat gttggttgtc attcgtggcc tgaggtcatc gtcgtccacg acgacgtgtc 155880
tatagcgtgc ggtgtgatca ttgtgtcgag ccagagaaag cgcgcctcgc acgacgtttg 155940
cggatcggct cgcgggtgtg tggaattcct aagaacataa tcagctggtc gtctttcttt 156000
gatgtgttgt tgtcgtcgag gtcttgcttc gttttctttt ttctttttag tcgatggaac 156060
ttttcttcgg tacgggttct tgttatggaa gcttgtgttt tcgaacatga attcgaaaaa 156120
ataaaaaggc ctatcttcgt ttcaaaaaaa ggacagatat caatcttctt aacttatatc 156180
atggtaaatt cagaatccta tggtgtctta ttatctctaa agtagtcaac attatggtct 156240
aacttgtatt tccctgacga gatatatatg atccttataa cctggctact atcatgaaca 156300
acaatatcct tacttacagt catcttcgtg agttaatgaa gtataatatc ggtcatctat 156360
caacttatct gctatgtaac gtaccctttt aggtattttg cgtttcttaa cgagtgtacc 156420
cgcctgtgtg aggcgaaact ctgagaagtc taccgagtcg agttacaagt cactaaaaca 156480
cttacacgag ttatctatac taaaatcact atctatgttg tttgcttacc taattattat 156540
cctacatgac gaagctacct cccaacgtaa ggtaggggga gaggagacag aacaataaaa 156600
agtaactaat gtttcttaga acttacccgc taaggactta ccaaactata ttcaccaaaa 156660
aacaacagct acgtgtttca tttgttttaa tctaccgaag taaaaaaaaa aaagatgatt 156720
agctatccag aacctactta cttcttaatg ttttaactaa ggatgcctat gggattggaa 156780
aaaaaatcac agcaacttgc tactaatcag ttgacagcga agagactcat aacaaagatt 156840
tctgggtaat acggttataa taatgcttat ggactaaagg atacttggaa aaaaagaacg 156900
ggctatgact atagagattc gtcgagatat taaacttcaa ataggcggct atcattcatg 156960
gttgtggtga ctatatcgtg gagaaaaaat gtgatcgtta gttagctagg tgagacttac 157020
agctatccat ccgtctagtt tttcgttgta atgatgatag tacgtctatg gtggtgatcg 157080
attttggtta acaatttgtt cgtttaaagg cttaatgtac ttatgctaca tgatgtatta 157140
ttctttgatt catcgttcct cctaaggggg tgtatgtatg tatgtactag tcgtatagtg 157200
ttcctaacat catgattatt cagactatgg cttcatctat cgtgtctaaa gttcacttat 157260
tctactatta ctatatatat gcactactat gtaactagga tatggtccta taaggtgtct 157320
tctatcacgg tggcttgttt atcgcttggc ggttacgagc aagagttcat cacggaccag 157380
ccgtgaggca gggcacacgc gggtcggcgg cgatgatgtc ccccgcgaag gggacaacaa 157440
aaacaagaca agaggccgcc ggccgcggcc acggacgcgt agcggttaca caatgtttgg 157500
ttgagcgttt tgtttcatcg tcgtggtggt ggttttgttg ttctctgtat atatcgtgtg 157560
gtggctttat cgtcatcatt attatcatca ttcttgtttc catcatcacg atgagttttc 157620
tccgttttcc tctcctccag tggtagtcgt gtatcatcat caatcatcgt agtgacgtcg 157680
ttgctgctgc tgctcttgcc ttcatggcgg tatttctctt cctcccccct aaccccatat 157740
taactcgtga gtgtgatggt tagagtggct gcttgttttt ttttcttttc tctttggaac 157800
aacaaaagag gataaagatg gtcggtgaat gtattattat tattatcatc attatgatac 157860
ggtcgcggtc ttcttctccg atgacgaaac ctgcgcacat cgaagaaaag acgagcgcgc 157920
gaaccgatag ccgtccgtct gggacgaagg agaagatgat ggggagagga ggagagcccc 157980
agaagccaga gcgagaaggg agacgacaga catacgtcgt caccgtcctc tggaggaggc 158040
acggcggcgc tgtttgttgt ttggatgctt gattatatcc tgttctatgg ggtagattat 158100
tatcaatagg cttggttttc aaaggtcagc ctgtgtattg tcgtgtcttt tttttcgttc 158160
tcatgatcgc ggagaccaca cagacgtgcg cgtctcccaa tggctaggcg ttctttttag 158220
gtagtaattt tttgatcttt tttttttctt aacaagtctg gcttgatttc ttttatctat 158280
gatcgattct tctttttctc gggggttgca tcttccgtga aagtaaagtg acactactct 158340
aaatggtaac catattatct gttgattagg agaaaaaata attttttcgc acgaaatcga 158400
tcctaagtga ggtgatttac ttgctatcac acgaaatgat tatcttttgc tgctaacgta 158460
ctgaattttt taacagaatt gcttctccgt aactatttcc gcagattcag acagattgtc 158520
aaaaaaaaat acggcacaga aatagtgggt ctgtggcttt tggttcgtgt acattcgcgt 158580
ttgcgtgtcg agatttctac ggtatgttta ttcttcctgc gatgatgtag ggtccttggt 158640
gtaagtagga tttcgagtat ctctcttaga gcgaacaaaa taatcaaaaa acaacagcta 158700
ggaaatcgag ggttactcta cgataaagtg tctctacaaa gtgaagaatg ttacgttgtg 158760
gtggaataat aagactcgcg tgatcgatga gtgatcgaga gcggctcgaa ccttctttaa 158820
gagctttgtt tagtgcaact ttaaattaca aggagtagaa agctgaaatg aatctatgaa 158880
ggtgctattc tttgaatatc ttactttgta cgcttcacat tcgttatttg gatagagagt 158940
tgtctagaga aaatctgtga ttctctatga gtgttatttt tattatcctt ttggggacta 159000
cgatttttct tcttgttcta cataccacta ctactcgtaa tcacatacat ggacgaaaaa 159060
aaaattcgtc aggcagtaga taccagattc tccgacgtta cggcgtcttt ttttcttttg 159120
agagagtatc tgctgagatt gtccgtggtg tatctagtcg ctatttttgt tgttactagt 159180
agttttgcac acagtttatt cagtataatt tttcttcttg ccatgatcaa ttgagcccac 159240
cacctttttt ttttagagag gaggaatttc gtcttgatct ccagccggag acaacggcgg 159300
cggtggtggt ggcgggagag atttcaaggc aatgaaaaaa aaaatttcgt tttgccatca 159360
agtggtgacg ataacccgtc agattgataa ttggttccta cagaaactat tctaaccgcg 159420
gaagaaagaa attgaaaaaa aaaattgaca aaaacatcat aacataaagg accacctacc 159480
tgggacgcgc agttgggcgg cggactgggg cggcatgctg cggcgatgct gtcggtgatg 159540
gtctcttcct ctctggtcct gatcgtcttt tttctaggcg cttccgagga ggcgaagccg 159600
gcgacgacga cgacgacgat aaagaataca aagccgcggt gtcgtccgga ggattacgcg 159660
accagattgc aagatctccg cgtcaccttt catcgagtaa aacctacgtt ggtaggtcac 159720
gtaggtacgg tttattgcga cggtctttct tttccgcgtg tcgggtgacg tagttttcct 159780
cttgtagcaa cgtgaggacg actactccgt gtggctcgac ggtacggtgg tcaaaggctg 159840
ttggggatgc agcgtcatgg actggttgtt gaggcggtat ctggagatcg tgttccccgc 159900
aggcgaccac gtctatcctg gacttaagac ggaattgcat agtatgcgct cgacgctaga 159960
atccatctac aaagacatgc ggcaatgcgt aagtgtctct gtggcggcgc tgtccgcgca 160020
gaggtaacaa cgtgttcata gcacggtgtt ttacttttgt cgggctccca gcctctgtta 160080
ggttgcggag ataagtccgt gattagtcgg ctgtctcagg aggcggaaag gaaatcggat 160140
aacggcacgc ggaaaggtct cagcgagttg gacacgttgt ttagccgtct cgaagagtat 160200
ctgcactcga gaaagtagcg ttgcgatttg cagtccgctc cggtgtcgtt cacccagtta 160260
ctttaataaa cgtactgttt aaccacgttg cgtcgtgacg ttgtttgtgg gtgttgctag 160320
gcgggctgga aagatgatgt ataaatagag tctgcgacgg ggttcggcgc tctgccggct 160380
gcggcggcac tcgctccacg gcctccgacg agcgttgcgc tcgcgctttg cgccgccgcg 160440
tcatggatct gcctactacc gtcgtgcgaa aatactggac ttttacgaat cctaaccgca 160500
tcctgcatca gagcgtcaat cagactttcg acgtgcgcca gttcgtcttt gacaacgccc 160560
gtctggtcaa ctgcgtggac ggcgatggca aggtgctgca ccttaacaag ggctggctct 160620
gcgctaccat tatgcagcac ggcgaggctt cggccggcgc caagacgcag cagggcttca 160680
tgtccattga cattacgggc gacggggaac ttcaggagca cctctttgta cgcggcggta 160740
tcgtctttaa caaatccgtc tcctcggtgg tgggctccag cggacccaat gagagcgcgc 160800
tgctcactat gatttccgag aacggtaatt tgcaagtgac ttacgtgcgg cattacctga 160860
aaaaccacgg cgaatcctcc agcggaggcg gtggttgcgg tgccgcgtct accgcctccg 160920
ccgtctgcgt gtcctcgctg ggtggcagcg gcgggactcg cgacggccct tctgcggagg 160980
aacagcaacg gcgaaggcag gaacagcgtc acgaagaacg gcgcaaaaaa tcgtcctcgt 161040
ctgccggtgg tggtggaggc ggcggcactg gtggtggcgg tggcggcggc gggagcggcg 161100
gtcagcactc ctcggactcc gccaacggac tgctgcggga tccccggttg atgaaccggc 161160
agaaggagcg gcggccgcct ccctcctccg agaacgacgg tgagtcccgg ccctcctcgc 161220
gtcacggtgc tttccgagtg gactcgtgag ccccccgtag cgcacgagcg agcaggcgag 161280
cggtgttggt gcgctggtgg ttgtgtggat gataaccatg tgctttttcg tgcgctatgt 161340
gtcgtcccgt ctgtaggctc tcctcccctc cgggaggcga agagacaaaa gaccaccgca 161400
cagcacgaag gccatggcgg cggcggcaag aacgagacgg agcagcagtc cggtggtgct 161460
ggcggtggtg gtggcggcgg cagcggccgc atgtcgctgc cgctggacac gtctgaagcg 161520
gtggcctttc tcaattactc gtcctcatcc tccgcggtct cttcttcctc caacaaccac 161580
caccaccatc atcaccacca taacgccgtg acggacgtgg ccgccggcac cgacggtgcg 161640
ttacttctac ccattgagcg cggagcggtg gtttcgtcgc cgtcgtcgac gtcgccgtcg 161700
tcacttcttt cgctccctcg acccagcagc gcccacagcg cgggcgagac ggtgcaggag 161760
tccgaggcgg cggcgacggc ggcggctgcg gggttaatga tgatgaggag gatgaggagg 161820
gctccggctg aggcggcgga ggcaccaccg cagtcggagg aggagaatga ttccaccact 161880
ccagtctcta actgccgtgt tcctccgaat tcgcaggaat ccgcggcgcc tcagcctcct 161940
cgcagtccgc gttttgatga cattatacag tcattgacca aaatgctcaa tgattgtaag 162000
gagaaaagat tgtgcgatct ccccctggtt tccagcagac tcttgccaga gacgtcgggc 162060
gggactgtcg tcgtcaacca cagcagcgtc gcgaggaccg ccgcagctgt ctccacagcc 162120
ggcgttggcc ccccagcagc cgcatgtccg ccactcgtca ccaccggtgt tgtaccctca 162180
ggttccgtcg ccggtgtcgc gcccgttgcc gccgcagtcg aaacaccagc tgctcctccc 162240
cggcccgtgt gtgaaatcaa gccctacgtg gtaaaccccg ttgtcgccac cgccgcggct 162300
gccagtaact cttcctcgtc ttcttcggct ccactgccgc cgccgccacc accgccgggc 162360
ggacgtcggg gtcgggcccg gaacaatacc cgaggaggcg gcggtggtgg cggtggtaga 162420
aacagccggc ggcaggccgc atcgtcgtcg tcctcctcct ctcggagatc gcgacggaga 162480
aacaaccgcc acgaggacga ggacaacgat cctctgctcc ggttgtcgca agtcgccggc 162540
agcggccgcc ggcgagggcc ctcgttcctc gaggacggac tcgaaattat cgatcccagc 162600
gaggaggctg cgatcgccgc cgcctcgatc gcggcgtttt tcgacgatta aaaaaccgag 162660
ccgagaccgg aaaaattatg aaacaggacg cgcttggaca tttgggtttc cacccctttc 162720
ggtgtgtgtc tatatatatt gtggtcactg attttttttt tacaataaag agatagacat 162780
cacagttcac catcttgtct ccccggtgtg tctattatca tcaatcaccc acagagtcgc 162840
cagtccatgg tctctcggta atgcgtgtcc agatacgcgt tggccagtat aaagtggtcg 162900
ttgcccacga aggcgcgggt ggtgttgcgc ggcgacgggt ggcaggactt aagtaccaag 162960
tgccgccgtc ggtcgatcag gtactcgcag gtgtgcgcgt cggcgcccca cagcatgaac 163020
accagatgct cccggcgctc tgacagcctc cggatcacat ggttactcag cgtctgccag 163080
cctaagtgac ggtgagatcc aggctgtccg tgcaccacgg tgaacacggt gttgagcagc 163140
agcacgccgc gtcgcgccca ggcgtccagg caacccgagg ccggacgctg aaacccgtcc 163200
accgtacgcg ccagttcgcg aaacacgttg ttgagggagg gcggcggcgg tcggcccgcc 163260
agcgtgccga aggccaggcc gctggcgctg ccgtcgcagt acgggtcctg gcccacgatc 163320
accacgcgca cctgctcggg cggacacaga tagctccagc ggtgtacgtg ctcgggtgcc 163380
gggtacacca tctcgagttg ccgcgcgcct tccaccgccg ccaccgtgtc gcgcagcagc 163440
accgtgtcgt ggtcgggcaa gctgaggaag cggatccagt cggcgctcag acaaaacacg 163500
cgagcctgct cgtcgggggt taacagagag cctttattat cagcaatgtt agcgagcatc 163560
cactgcttga gggccatagc gcgagtgagc cggcaggttg acgcgcgtct gcttcagctc 163620
gggcggcagt ccggcgtagt atttatctag gtggcgtagc agcggcgggt ccagctggtg 163680
acgcaggcag aattccttca ccgcgttgta caggccgtaa aagagcgtga tgccctcggg 163740
cgcggcagcg gtgctcacgg gcagacgcac ggcgcggttg gtacgcgtgg cttcgttgcg 163800
tatggccacc accacgttaa agagagacgg tggcaccagc tcgaagccta acacgtgttc 163860
cgtgaagatg ctgcgcccgt atgacagtcg cgtgaggtcg tagccgcggc acaggtcgtc 163920
cacgcacgtg tacacggccg gcgagccatc gccgcactcg ctgtagccgc gcattaccgt 163980
catccagcgc ggcgctgtgt ccgagcttaa cagcgtcagc agggcccgca attgatccgg 164040
attgttgtac agcagggcca gagtgtccag gaaagcatcg tccaacagca cggagttggc 164100
ggcctccggc gtaacgggac ggtaacggat aagttgcgat agcgggccat cgcgcccggt 164160
aacattcacc aacgggcgca gccaactttc atacttgtca ccctgaaaca cctcacccaa 164220
caggcatcgg cgcgttagtt cggggcactc cgcggggact ttctcggcgg cggtaggagc 164280
gacgctgacg gcggctgagg aaacaatgga cagcagaagg caacaccaca gcagtatcac 164340
cggtccaggt gagaaagaga agccgcaatc cgggcggcgg cacatcaagt ctgcggcacg 164400
atgagagtgt gacggtaagg agccagttgg cgccgaaagt tggcactcag gtcttcgatc 164460
cctaaaacgt tatatattgc atccagcagg tgagccaggc taaacggatt cacgtaccag 164520
gtttggttac ccgcgacgat gacggccaga ccgtgggcgc tacagttgga gaggttcctg 164580
ggtacgaagg taactgagtc gatgtcgcgc cacgggggga atgagacaga cgactggcgc 164640
acgctgtaat cacaactgtg attgacgtat tgtagcgtgt aatttaggtt gcactcagcc 164700
tcgaagtaga gggggaacca cagttcgtcg tactcgtcgt cgtcctccag ttctggctct 164760
tcttcatcca ccgcaatgtc tacgctgctt tgagattcct cttcgtacag gatgattgac 164820
aggttatggc tacaaaggtc ctgggcggga ggacgcgtgg gagcgcgggt ggtggtaatg 164880
ttttccagat caaaagttgg agtgtagtcg gatgttacat ccccgttgtt ggaggtggta 164940
gaagttgcgg ccggtgtcgc ggtggtaagt atggatacag aaggggaggg ggaagtagcg 165000
ttcgtaccga tggttgtggt attattattc cctgtgtttc ttgttccaga aaccgttgac 165060
gttgagatgg gaatcgacgt ggtgctggac gtcagattgc tgaccgagga aaccgtggtg 165120
ggagtggtga cggtgttact cgtggttgaa gtgacgttag gggaggtagt agtggtaccg 165180
gtggtggcga cggtagtgtt tgtcgtggcg gcggcagcgg tggtaccggt aacggtggtc 165240
gcgttggttt ccaccgcttc acacagtaag caaaagcaca gagccaggaa aagcaaccag 165300
ccccgccatc gccgccgccg cttcatgagg tgggcaggcg aaagctggtg aattcgttgt 165360
acagcggcaa gtggggcgcc gcgatcgaag ggtacgtcaa caagctgacg ttgatattaa 165420
atacgtctgg ctgcttttct acgatggaag cgcacagggt tacggcgtca aacaggtctt 165480
tcttggtggc gcccgagacc cacatctggt atacacccgt ctcgtggtac gaagtagagc 165540
gcggcaccac cggacggatg cagtccagaa cgcggttggg atcttggtga aagaatttga 165600
acgtggctac ggcctgtggc gtgtgcggca tcgtctgcgt gatgagctgc tggcccgcta 165660
acacggtgac gttgtgcaac ttgagcaggg cactcttgag ggcctggaaa gcgttgccgc 165720
acgaggcgct gatctgcagc tgcacggccg tggagtcgtg cagccgcatg agacgtgaca 165780
cctcttcgaa gacgtactta tacttactgg caaagagtgg cgcgtatcga cagtcggccg 165840
gcaaaatgta ggtggcgttg ccgccgttgg tagccacggc gggcgcagcg gccgcggagg 165900
ccggcgtaaa cagcgtcagc ggccggtggt ggctggtaag gtcgatcatg ggcggcgtgg 165960
tgaccgtggc ggtggcgggc atgacggggt ttgcggcgac gggcactccg gccacagcgg 166020
cggccgcggc ggccacggcg gcgctggccg agcccacacc cgccggcagt cctccgctac 166080
ccatgacgcc gccgggcaga gcgtcgccca gacagacttc cacagtggcg ggcgcgctct 166140
cggcggtcag tacggtttgc cgatcgacct cgcgacgaaa gctggtgagg aactcactat 166200
gatccatggc cgcagggccc gagatcccgg gattctgcgg gtgctgaccg agtgcgggcc 166260
gagttatatg gaagacgatt agcttggagc ggagttttgc gtccctagct gacctgcgga 166320
tcagcgacgt accataggga tagactgtga gcggcggccg caacggcggg gtcggccgcc 166380
gttcgtcgtc acggggcggc gcgagggagg aggaggtggt gggtacgatc ttgacgtggt 166440
tgacgtcctg cccgtccggg ggaatacgca aaaaaacccg tcgcggcgct accacgatgg 166500
tgcgatgggt ctttctcttg ttggccgggg ccagggactt gcagatgcgt gtggagccgt 166560
agacgatctg gacgtggtcc tgggagaaca tgaccatcgc cgccaacgct cagcgggggg 166620
acgtattggg aacacagagg atgagggaaa actccgtaga agtcagcgaa ataaagacaa 166680
cacagcagcc actcctctcg tctcgggccc taccactgct tgaagtaggg caccgggtgt 166740
ttcttttcct caacgggctc ctccagtctc ttataggacc agtcccgccg gcgcgccagc 166800
atgtaggtca cgtacaaaag aataatcacc atgaacacca ggaaagccag cacgccgtag 166860
gccagcagcc ggtcctcgaa cagcgggtcg ctcttgataa acacgtaggt ggtggtaaaa 166920
cttcggcccg cgatctgaac gtggagacgc acgacagtat acgtgccgtt gaggtagaag 166980
acaaactcgc gtaaccgttg tccgttatac gtcacgttac taatattcca cggcggaatg 167040
agctggttgc cctgatgcag atgcacggtg ctgttggggt gatagaggct gctaccgttg 167100
agcaagcagt gttcgtgttc ctgaagcagc acgcggaccc gcatcgtggt agcgttcaag 167160
cgagtcccgt acacggcgta gatgggatag gtgaaaaggt cccaagtggc gttgtgatgg 167220
cggccccagc tgaagaaaga gcacgtgtac tcagtggtct cctgcggcct gagtcccgag 167280
ataagcagct cttgagcagt agcgttgtag gagagatgta gttttcctgt ggaaaaaatt 167340
aatgagttgt ttattttgtt agcaggttgg cgagggagga aggagaacaa aacagaaagg 167400
tacgtgttac ttacctttat cgttggaggg aaaagcgcta agatacccca cctgagtgaa 167460
gggacccttg cagtctgtcc gtgcataaca agtaactgat aaaatgtctg gatttttggt 167520
attattcaac aggattactt tgcaggtggc gtttagagac acttggtcgt agctgtagct 167580
ggcttcgcaa ttcacagtat acaggtgccc ctctttctgc gtcgtggcta tcacggaggt 167640
ggaggcggac gaggtagagg tttgtaccgt ggtggtgaca gcagaagtga cgttgttaga 167700
ggtacttatt gacgtagtag acgtgacggt ggtattacta ggggaagtga cggcgcttgt 167760
ggtgctactt ttcactctcg ggtgcatgtc gcccaagagc gcaactacga gcgcgatcgc 167820
cagcacggaa cacatgttgc cgtgtgacga gacggcgtgt ggacgagcta tatgtggcag 167880
gaggtcgcgt cacctcttgt gacgcctaaa cgtccagctc cagataaaag aggcgttaat 167940
aatgaagacc acaaaaacca cttgcgtcag tatgacaatc ataaaggctc ggtgattgct 168000
acgcctaaag tacgcgggat tatccaccag ttcatcctgc tgaacaaagt ggatgattga 168060
cgtgctggtg ttaccggccg tcgtattgat catggatttt actaagaaag ttttggcacc 168120
aaaagtcccg ttagagcccc agcaggtaac gctgccgttc acataggctc ccggtgcccc 168180
tgtcagcatg cgtttcagtt catgagtata atttttccta tcgttataca tatcatcact 168240
gtagttgact ttgctggtga gaaactgtgt gttctgtgga atactgatca tcatccccga 168300
ggccaaaaag ggcgaatcgc aagctgtagt gttacagaaa atagtcaggt tagtgtcatt 168360
atgctcatac atataagcca cgctaacctg gggctcatac cacccaatcg caaccgccag 168420
cacgtcccat ctcccgacat ttatcaccgc caccactaac aacgtcaccc ccgcacggta 168480
catagttacc ctctcgacgt cgccggctgt caatgacgtg cctgcgtcag tggctatgat 168540
ttatagcttt tggacacaac cgcaacggat ctgtcgtaat ctaccttcca cagggccgcc 168600
gcgacgatgc tgaacgacag gatcagacag acggcgtata ggagtcctag gtcggcgtcg 168660
acgcggcaag tgcggatgtc tcgcagggtg ggtagatggg cgatgcacaa ctctttctcc 168720
ccccgcacgt acatcccatc tcgtatcagc agccgtagcg tagcattaat ggtcagcggg 168780
gtaaccaaag aaatcacata gggatgtgta caggaagtac agtgacgggt atccgtgaga 168840
tgtaagtcat cacccttctc actgttatca tgaaagacca ggactcgggt aagacgaccc 168900
gatgaatact ggatctccca ccacagtctt tggtccaaca ccgagagggc acaagagatt 168960
ctaagtctcc ctgggttggg ggagcagatg taagccccgt gtgtgcccct cgccatcaga 169020
accatacaca tgagggggaa aaggacaagt atccgggacc acccgcaccc ccacatcacg 169080
agaccagaga cggagatgta taaaaaaaag ctacttttat taaacagcat tctcaccaca 169140
cgttaatact gtcacgggga atcactatgt acaagagtcc atgtctcttt ccagtttttc 169200
acttactgag acttgttcct caggtcctgg atggctgcct cgatggctag gctcagggtg 169260
tccaggtctt cgggaggggt ctcggtgggc tgctcaaact gccccacgtc gtaggccttc 169320
gcggccgtct cgtagatagg cagcatgaac ccaccctggt tggtggagaa gatgcgcacc 169380
atgacctgtt tgggaaactt ttgcatcagg ggcaggcaca ggttgagagc gcccaacagg 169440
tccacggggg tggcagcgtg gatgatcatg ttgcggtaat cggaagaacg ggggcataat 169500
tggtgggtgt gcaattcttt gaggctccac gcggccttga cgccttcgtt acaagcatcg 169560
gccgtgcgct gcgccacttc gggtgggtgt gtcacaggca tggtgtgctc catgagaaag 169620
ggagtggaga gggccaggtt gcacatggtg cccaggcgac accgcaccgc atccacctca 169680
ctcttcacct catgattgcg ggtgtagatg atctggatgc ccttgttgtt cacctgcatg 169740
gttttgcaag ctttgatggc ctcatctaac acctggtgca tactgggaat catgaagggc 169800
aggttcttgt attcaagaga gcgattggtg ttgcggaaca tgcggctcac ctcgtcaatc 169860
ttgacgcgac cccgccgagt ctgcacgttg ggtgtgcaga agggggtgtt cttatctttc 169920
atgatattgc gcaccttctc gttgtccaac tcggagatgc gtttgctctt cttcttgcgg 169980
ggtccggtgc tcgccccgcc gctgctctga tggccgcagc tcagcagaga ggaggaggcc 170040
gcgccaccaa aaccgccgcg cccatggtgg ctcgaggtca cggatgctcc tccgccactg 170100
ctgcatttca tctcctcgga ctcactctcc gagtccgaag ccgaactgca ggaggaggaa 170160
gacgaagagg aactatcttc atcgggccgg cccaagggat cgggaagagg agggtggttc 170220
atctgggaga gcgggtgcgt gggagaggtc actcgcggcg tgccgctgcc ggtggaaggg 170280
gaagacgcgg tagcaccgcg ggtttcgact tcttcaccct gttcttcctc gctatcagag 170340
atcacgatac agccggcggt atcgataatc ttgttgcggt actggatggt aaagtcgggc 170400
tcgggcttga tgtcttcctg tttgatgagg aggggcagca tgataggcgc gggaggcacg 170460
ggcggtttaa taatcacctt gaaaggacgc gtggttttgc gcggtttctt acgcgggctg 170520
agctcgggag tagcggatgc cccggggaga ggagtgttag taaccgcgac gctggtgggg 170580
gtcggcttgt taagaggggc gctgctaacg ctgcaagagt gggttgtcag cgtgtggccg 170640
gtgctactgg aatcgatacc ggcatgattg acagcctggg cgaggatgtc acctgatggt 170700
gataagaaga cacgggagac ttagtacggt ttcacaggcg taacacgttt attgagtagg 170760
attacagagt ataacataga gtataatata gagtatacaa tagtgacgtg ggatccataa 170820
cagtaactga tatatataca atagtttact ggtcagcctt gcttctagtc accatagggt 170880
gggtgctctt gcctccagag gtggtgggtt cctcagcacc atcctcttct tcctctgagg 170940
caacttcccc tatctcagac actggctcag acttgacaga cacagtgtcc tcccgctcct 171000
cctgagcacc ctccccctgt tcctcatcac tctgctcact ttcttcctga tcactgttct 171060
cagccacaat cactgaggac agagggatag tggcgggtac aggggactct gggggtgaca 171120
ccagagaatc agaggagcta gcaccagcgg tggccaaagt gtaggctgca atagcatctt 171180
cctcatctga ctcctcagcg atggcccgta ggtcatccac actaggagag cagactctca 171240
gaggatcggc ccccagaatg tactgggcaa agaccttcat gcagatctcc tcaatgcggc 171300
gcttcatgac attgataacc tcaggcttgg ttatcagagg ccgcttggcc agcatcacac 171360
tagtctcctc taagatatag cagcacagca cccgacaaaa ctcacttaag agagagatgg 171420
acccgtacat ggtcatcatg caagcgtcac tggtgacctt gtactcatta cacatggttt 171480
ccacacatgt agtgaggata tccataaata tgtgatcaat gtgcgtgagc accttgtctc 171540
tctcctcatc caaaatctta aagattttct gggcataagc tataatctca tcaggggagc 171600
actgaggcaa gttctgcaat gccgccatgg cctgactgca gccattggtg gtcttaggga 171660
aggctgagtt cttggtaaag aactctatat tcctgtagca catataaatc atttttctct 171720
taagttcatc cttcttagca cgggccttag ccttcagtgc accccctaac ttgttagcgg 171780
cgcccttggt cacatcatgc agctccttaa tacaagccat ccacatctcc cgcttatcct 171840
cgggtacaat gtagttctca tacatgctct gcatagttag cccaatacac ttcatctcct 171900
cgaaaggctc atgaacctta tctaagatat ctaaggcatt ctgcaaacat ccccccatca 171960
tattaaaggc gccagtgaat ttctcttccg tctgggtata ttttttcagc atgtgctcct 172020
tgattctatg ccgcaccatg tccactcgaa ccttaatctg tttgactgta gaggaggata 172080
acaacacata taagtatccg tcctcctgac tcatttatcg ctatctcgat gccccgctca 172140
catgcaagag ttaatcttca ctctatctga catacacaag taaatccacg tcccatgcag 172200
gttagtatat atcacataca tgtcaacaga cttaccgagt tctgccagga catctttttc 172260
ggggttctcg ttgcaatcct cggtcacttg ttcaaaggtt ttgagagatt cttcggccaa 172320
ttctgggaac agcgggtctc ccaggctcag ctgactgtta acctccttcc ttaacatagt 172380
ctgcaggaac gtcgtggcct tggtcacggg tgtctcgggc ctaaacacat gataaacaaa 172440
gtcataagca catgggtcac atacaggaaa tatgtatata acattaaaga tataactttt 172500
tattaaaaaa aggggaacac aagtcccgac acgtaccgtg gcaccttgga ggaagggccc 172560
tcgtcaggat tatcagggtc catctttctc ttggcagagg actccatcgt gtcaaggacg 172620
gtgactgcag aaaagaccca tggaaaggaa cagtctgtta gtctgtcagc tattatgtct 172680
ggtggcgcgc gcggcagcaa cgagtactgc tcagactaca ctgccctcca ccgttaacag 172740
caccgcaaca ggagttacct ctgactctca acagaacaca actcagctgc ctgcttcttc 172800
tgctgctgct gccttaaatc ttccatctgc gtcagcggtg caagcccatt ccccgagctc 172860
attttcagac acatacccta ccgccacggc cttgtgcggc acactggtgg tggtgggcat 172920
cgtgctgtgc ctaagtctgg cctccactgt taggagcaag gagctgccga gcgaccatga 172980
gccgctggag gcatgggagc agggctcgga tgtagaagct ccaccgctac cggagaagag 173040
cccatgtccg gaacacgtac ccgagattcg cgtggagatc ccacgctatg tttaataaaa 173100
actgcgggca ctggggacgg tggtgttgta tatgtgaatt tgtaaataat aaatgagacc 173160
ccatcctgta aaaatacaga gtccgtgtca gtctctgaag gacagagtat tggcatatag 173220
ccaataaaga tagttgtggc aaagagccat gttatggatt agtaatggaa agtatcgtca 173280
ccaatagggg agtggtcaat aatggtcaat aacccacacc tataggctaa gctataccat 173340
cacctatagc ataaggaagc gggggtgtat agaccccaag ccaaaaacag tatagcatgc 173400
ataagaagcc aagggggtgg gcctatagag tctataggcg gtacttacgt cactcttggc 173460
acggggaatc cgcgttccaa tgcaccgttc ccggccgcgg aggctggatc ggtcccggtg 173520
tcttctatgg aggtcaaaac agcgtggatg gcgtctccag gcgatctgac ggttcactaa 173580
acgagctctg cttatataga cctcccatcg tacacgccta ccgcccattt gcgtcaatgg 173640
ggcggagtta ttacgacatt ttggaaagtc ccgttgaatt tggtgccaaa acaaactccc 173700
attgacgtca atggggtgga gacttggaaa tccccgtgag tcaaaccgct atccacgccc 173760
attgatgtac tgccaaaacc gcatcaccat ggtaatagcg atgactaata cgtagatgta 173820
ctgccaagta ggaaagtccc gtaaggtcat gtactgggca taatgccagg cgggccattt 173880
accgtcattg acgtcaatag ggggcgtact tggcatatga tacacttgat gtactgccaa 173940
gtgggcagtt taccgtaaat actcctccca ttgacgtcaa tggaaagtcc ctattggcgt 174000
tactatggga acatacgtca ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc 174060
aggcgggcca tttaccgtaa gttatgtaac gcggaactcc atatatgggc tatgaactaa 174120
tgaccccgta attgattact attaataact agtcaataat caatgtcacc atggcggtca 174180
tattggacat gagccaatat aaatgtacat attatgatat ggatacaacg tatgcaatgg 174240
ccattagcca atattgattt atgctatata accaatgact aatatggcta atggccaata 174300
ttgattcaat gtatatatcg atatggattg gccatgtgcc aacttgatgt cgcctctatc 174360
ggcgatatgg cctcatatcg tctgtcacct atatcgaaac tgcgatattt gcgacacaca 174420
gaatcgccca ggtcgccaaa gtcgtctatc gccatccccc gtaaacgata taagcgctat 174480
cgccagatat cgcgtatgcc caaaaatcac ttttggaaaa atggcgatat cagttacaca 174540
gagactcaca tcggcgacat tttcaatatg ccatattttc aaatatcgat ttttccaata 174600
tcgccatctc tatcggcgat aaacaccact atcgcgcgac atgaatttag tcggcgacag 174660
aaatctcaaa acgcgtattt cggacaaaca cacattttat tattcactgc agcatatagc 174720
ccattttagc gcggcacaca tccagccgtt tgtgtttctt aacgctctcc aggtactgat 174780
ccaggcccac gatccgggtt atcttgtcgt attccaggtt gatccatcga tagggaacgc 174840
tgccagcggc gcccagcagg tactgcgcct tgtcgttcac tttgccgcag cgtattcgcc 174900
cgtcagcttc gaggtataac ctacaacacg gaagggaagg ggggtacaaa acgtgaaatt 174960
agactttttt tttaatgatg ttttgtccct ctctgtctta ctctcccata ggctgtaagg 175020
ccctcgagga agagacttac ggattgtagt tgcagctcgt cagtttgttg tgtacgacct 175080
ggcgtgtcaa tgaatgggtc atggtggtga tgatcccgcg aatctcagcc gttttctcgg 175140
gactgtagca gacttcgccg tccggacacc gcagcctgtg gattcatgaa aatctactct 175200
ggcattcccg aggatcgtcg atggaacatg gctatcagaa acgtcgagag acagatccag 175260
acgcaccaca gaacgcagac aatcatgaaa atacgtacgc gacggtgaag cgattgcaca 175320
ttttgaaatc gtaacagcgt tccggcgggt ggttgacgtt tatgaattcg caacattctt 175380
ctgcgcgtac ccgcggcacg cggctgtgac ccagtagcaa ccacaacgtc gtcaagaacg 175440
gcgtcaggtc tttgggactc atgacgcgcg gttttcaaaa ttccctgcgc gcgcgacggg 175500
ctcaaacgat gagattggga tgggtgcaga aggtgtaagt ctggttattg gcctcggtga 175560
acgtcaatcg cacctgaaaa gacacgctgt agtcccggaa gacgtgggcc cagctctcca 175620
gtttcatcac acacatctga taacgtgtgc catcgttgac gacgaagcgt agcagcttgg 175680
tctgcttggg caccatgtgc gctccaaaaa tcttggcgtc ttccacgctg atctgcacgt 175740
ttccgtcgct cggtttcgaa gccgttcggg gcatccgttg gaggatggtc tggttgcgac 175800
cgctcagata ccagatcacc tttttcaccc aggtggagct tttctccacc aaggtctggc 175860
cttcccggtt gtacagcaga tacagggtct cgttgcgaca ctcgggaccc gttgataccc 175920
gctggaaccc cgagaattgc gagggggacc gtgggggcga gggatagaga aaaggacagt 175980
aaaacgtcgc cgcgtcatgc ggtttggaat acgtcagttt agaccatggc ggggacggat 176040
tctggttcgc cgttagcgtc gaccacgaag acgccagaca gggcgttgcc caaaccgcgc 176100
acagaagcag gcagtgaaag tagtgacgaa gcagaagccg cagcatatta tttcccgtga 176160
cgcaggctag ttggcaaaga gccgcacgct gaactcgagg ctccgggcgt gcggcgccag 176220
cgaaccggcg gcgttgaacg tggtcctttt gttggtgccg ccgcgacggt tctgacgtct 176280
aaagtcgctg atgagcaacg acacctcggt cacgttgatt ctgcaagcac aggttccgaa 176340
cgtcatttca caccccatgc ggttacctac ccgttacccg ttcgccctta ccttcccgtt 176400
gtcatacacc tttagcgcgt accctcacct cttgagcacg tcaaagttgt ccaagccgtg 176460
gctcgcatcg tagtggtagt tcaacgtgag gtccacgagc tgttccacat acttgtaacg 176520
ggtttggtcg ggcagcgcgc gagagcacgc gtcccagtaa tgcggtactc ggtaataatc 176580
gtttttttct gcggtctccc gctggcactg acccagcacc acggcgcaca gacaaacaga 176640
cagccacacc cgatacagcc gcatgttgca gactgagaaa gagagcttta ttatgagaca 176700
tcatacacat agtataggcg aggtaatggg gcggggaaag agttggaacc gaaagacaaa 176760
aaaaaaagcc tagtcgtact cgggatctct gagcgagacg gattgcgtag caactttcat 176820
tagtttggga atctgccagc tggtgctgtt ggaaggttct tccatttccg aggcggtcag 176880
ttcatcgtac accgagacgt agtacctgat ggggtcctcc tcattgtccg agaggtgaga 176940
ttcgatggtc aaaggcgagc ctctcccata attgggattc acgaacgacg tgtccaagtt 177000
gccatccttt ctgaaataga tgacgttctc aggatcatgt ttcatgcgct cgcgggccgc 177060
ggacgcctcc tcctcctcgt cccagtcccg agtttccaac cgctgataag ggctcgagga 177120
acaaaatccg gcggggatct gagaacctcg tcgggaaccg ctgccaaacg ggctgctgcc 177180
gccactatcg tccgtgtcgt ccaacaggtt gacggcctct tcgtcggcga aacgaaagcg 177240
gcccgggtgc ttgcaacacg aggagtaaac taccgcgatg agtaccgcta tgaagctgaa 177300
aatggaggtg cctgtcacaa tgtagaagag gatagccagc actttcatga tttcgtcatt 177360
gcgcgcgtcg tgaacggaag attcgcgggc ggtggtcatg ttggtttcgg ttgtaggttc 177420
gctactcgta gtgctctcga cggtatttct gctgctggtg ctagtaggga cgtttgtgct 177480
gctggtcata tttgtagcgt cgctgaagtc catgtgaagc agcaacccga acgcgaccag 177540
gaccaggaat gttgcgcgaa ggagaccccg cggggccggc attcttgaga cgtggcgacg 177600
tggatttctt gttatgtccg cgaacgacgt gtgacgagga cgtggtttcc gcaagcctct 177660
accgacgccg cgacaccagg taggttatga aaacgcgagc ccatatcgcc gccatcattg 177720
taatcagcaa tgtgttgagg tactgcacga tgaatctgtc tagtgacacc agccaaccct 177780
ctgcttttgc gggcaagcgc gctttcggtg acagggtgta tcgtacgtag ccgcgggtca 177840
ggcgcgcgtt gtagcggtac acgcagaaat ctatccacag gccaacgccc ggctgtagct 177900
tcggatggtg gataatagcg cggtgacgta cgccgcgggg ctttagaatc tccacctgta 177960
aggccatctc ctccaggtag tgggtctgac tgcgacgcag cgtccagttc atgtaaaagt 178020
cggtctcgcc gtgtccggcc acgaagaggc tgcttactaa atcgggcgcc agagctaggt 178080
caggcgtatc aaattccact gccaggcgac ctgattctaa cggttccacg atccgggaga 178140
gcgtttctag atatagagca aagcgtacca cgtctacctg cggtgtaaaa aactgttgtg 178200
ggcgttcacc gtcgttgacc acgtaggcca cgtagaggcc aacattttcc accacgggtt 178260
ctagctgcag gcggcacgta aagcttagaa acgacggctg tacggtttgg ttcccgtgaa 178320
gctgaagcgt cacttccttg ccggggctca ccgtgctgta acgtcgcacc gagtcggtca 178380
tctgctccag atcggtagac cagaaaggcg tgcaatgcat actgtcccag tcgcgacaca 178440
cagcccagcc tagctcggtg aagggtcgac gcacacccga aaaagtgtgc ttgaagacca 178500
gggggtcgcc tcggtagctc agtagccgaa catgcacata gtcgcggcta gcgttgacag 178560
acggcccgtg gagggccagt aggacgagcg tgaacagcaa gcgcaacatg ctgcgcgggt 178620
taggaaatgc ggcgtgccgg ccaccgcccg actcataaac gctaccagca tgacgtctca 178680
gatcacacaa gtgacgagga gcgtaccgca aatcactagg gaaaaggcca gcagagcccg 178740
atagtcttgc tcttcgcgaa cgatctcgtc cggttcctcg cagtcttcgt ggtccacaga 178800
agatgaggag caggattctt cgttaatctc tgccaggata ctagtgctat accacaccag 178860
agcgctcagc gtgcccaggg ctaccgcacg gtaaaatagg gacatgatca ccagcgcagt 178920
ctaaagtagt ggtaattaag tttcttggcg tatttccaga gaaaggcttt gtaggccgta 178980
gggactggcc aggcaccgaa ctcaatattg gtagacacta cgtcgtaaat gcgttgttcc 179040
tcatctaaga ttaaccgaaa aaatagccgg ttgatgtgac ggcgcacggc ttgcgcgtta 179100
ggattgagac acttggtgcc cttgtccttt aaaatagcca gcacttcctg acgattgcag 179160
ctttcgctcg ctgcgattgg cttaagcagt tgagttccga ctggcagggt attcaacaga 179220
atttggttgt tgcaacggca gcgcctgtcg taatcttcta gttctaaaac atggacggct 179280
aggggacata tggtaagtaa catatatgcg attaatgaca ggtatcgtac cgataacaga 179340
ttgatatgcg agtttgaaac cggatggtgc aaccatgtta gtaccatatt aaacacatac 179400
tgtaatattt tgttttaacg aacttgtctg tttgaaaaca tacattaaat attatcctct 179460
aacacctatc aaggttatat ttattcgtct cggttctggt gatttcgtta tgttaacatt 179520
ataccaccta ttatcgttgc gtttgtctaa ccattttgag aagaggtgat cgggcgataa 179580
acatactcca tgtccaggcg gcttccttcc gtctggatac aataaatgtt catttttatc 179640
gcatccgggc cctctgggat cgcgatgaag ccaataatta cccaatataa ttttattagg 179700
cggccatttt ctatgaagac atctgcagcg taattctgtt ccattcacct cataatgata 179760
cacatatgct aaaaaaataa tcaacgcacc aaaaattaat cgcattataa ttttattatc 179820
tacgtcacta ccagtaattc gtaatatccg gtattcccgg aaaatcactc aaaactgcgt 179880
ccatgacaca tcaattcccg ataagtaccc ccctttgaaa tcggatcccc ccacatacca 179940
atcaatcaca caacacacag gtttaaaaat cgatcacacg tcaattaggt ttcaaaatcg 180000
atactgttta ttatcaggaa tctagactaa ttctacaatg acagctctga atttctctct 180060
tgtctttctt gtcaggttct catcatcagt catcacttcc acccatcgag gagtcatcgt 180120
cgctccaaaa tcctttgggg tcgctagttg gaaaagtctc tgacacgatc caggcacccc 180180
gcacccagtc cgactgatct agcttgcgga gcatctcaac aggcatgagc tgcagggcca 180240
cggctgtcac ggcactgtat cgatgtaaca ctagggactt tctttgcgat gtagccatca 180300
acacggcgta tgccccatag ttcgcgtgat acgacgcatg atgggttaaa cgttcccatc 180360
cggcagtgcc gtctcgggtc cgtgcacaca acagctgcac agcgttatga tgcttaaaat 180420
taaccataac gctgggacta ctgatgaagg agtagtaatg agccaggacg ccgtacatcg 180480
aaggcaacaa gaaagagtga cagcacgata gcaccgggct cttatgtagg cgacagctta 180540
tttttcctga cgtcggcaaa aagtacctaa attccccaca gatattcaga cacggttccg 180600
taaagtgctt ctttttttag tgcaggaatt ggaaaaaata ataaaaaata tgaacagctc 180660
atctgtaatt atctgtgtga cttcatcgta ccgtgatgta aaaacaacaa caggaagctt 180720
acagggtgcg gtagaaaaat ttgccgattg tgcaacactg ttggcatctc tcactccgat 180780
aggcggctat aagatagaga attaaaagta tgatacccac aagaaagatg aagagggaca 180840
accaggctag agtatgacga ccgcttttcc tttgtttgac ggttacatgt gcggtatgat 180900
tttgctgtcg ttgcttgtga tgttggacac ctggagtgga aaacgacgta tgattcttag 180960
atgcgcatat ggtgttatta gtggaagtgc agttacgaac cgtgatctga gtgtcgttac 181020
attgagtaca attagtacag ttgtaaagcc ctgtgagata agtaccgttt gggcacagtg 181080
tacacgttat gccactattc tctgtacaca cttttgtaac tttttgtcct gatccgcatg 181140
gcgggcaaca ttgattaccc agcttcacct catcgggctt acacatttta cttcccccaa 181200
gctgtagtaa aaacataccg aagcagatga gcatcaccag aggcttcatg cctcctaccg 181260
gaagaataaa aataactcat agggccgaac ggtgtcatcc tctccgcggt ttgtaatacg 181320
agattgcaaa cgtaaataaa tgacataact tcactaacac gcatactaca aagtccacct 181380
acgacgctga aagttcttcc aggacagaac aggatagtca gccatcttca cagtctacct 181440
cttaggccgt atccaggagc ataggtaatc agtttccagc cacagtacag cgagcccagg 181500
aaaccgcaca cggtccctgc cgggaacacg taccaccaca tcgattcgtc gtgccgtaga 181560
accgtagagt tttccgaact tttatacacg ccggtggcgt tagggccgtg tgtgctgctg 181620
tgattggagg ttttgtgagc taggtaacag ctgtgatttc acctgtcgcc aacactgaca 181680
gcgattaccc aggtggagca caatcacata gctgatggac gttggttgat ccgttgattc 181740
ccatggacat tttaacggcg acagtacagc tcccgttaaa cattagaata atagacgtta 181800
gtggataaca gcatgttatt cgcccaagtg tgatcgtggt tatacacttt cttgtttttt 181860
gctcatatgc tgtaaggtgt tcgaggatcg tggggagtat atgtgttgaa tcggaatcat 181920
gtttactgac cgcgccatac ttcgtatacg aacctaaccg gcgtaaagtg ttttccgata 181980
tataaactgg cgcctattgt ggctgtagcg cccataggta tggcgtatac ccacggtgat 182040
gttgtgttat tcgttttttg tgataaaacg tagcttatgt ttaacgtgtg ttccgtcacg 182100
ttatgtgtgt cgttaaaaga cggcgcctgt acagtatggc tttgagttgt atcttgaatt 182160
gttattgcat ctggaggtgt tgtgtacaga gtggttgttg cgtgttgagg tgttgttacg 182220
ttttgaggca cagttgcggt gtacacgggc tccaaggtgt agttacggag tctttctatg 182280
caggtagtgt tgagatattt ttgaatgctg gttatgttcg attctgtgag gttaaagtgt 182340
gtactattta tgacggtgta atttagacgg tcttgccatc ccgaggatat tagtgttagg 182400
taattcgtgt tgttcacgtt tgcttgatat gtataggtag gtgtactgtt tgtgaggtcg 182460
caagtgtgat tttcttgcag agattttatc catcttgtgt gaaaatattg agatacgcga 182520
tgaatgtttt cgctatctat attataaagc gtttcagtgt cacctagggg ttgtttgttg 182580
taacttttat tttggaccct gggtgtgaac catgattcca atgtttgtat agtaaggtgt 182640
cctactaata aagacgaact gattcctacc gtaatgttat accgcacacc cagggtgccg 182700
tttacaaaca cggaaatgtt tccgttacaa accacgttgg cagatgaatt agattccagg 182760
tggtaacgat aggataatga ccgttcgctc ccaacggatg acacaaagta tccgaataac 182820
caacacgccc attcaatccg catattttaa tcacactatt cacacctcac acactgcatt 182880
ttttaacatc ttattttttt attttatgcg tgttctcacc tcttcatctt tttaacaccg 182940
gggtaactat cgtaagtcgg taggcgtcga tagccctcac cacctcgtcg tccccttccc 183000
ggcgtggggc accagcgtcc acagcactgc aggtaacaca ggtagcatag gaaacatacg 183060
gtgaaaatac tccaaaatcc caaaaatgcc gcgattcccc gagtggccca gggagacatc 183120
ccggtgtcta tgtcggccgg cggtgctggc gtcaccggta aaaatttcgg cgggtgtggc 183180
tgcgaacggt agcagtcgcc ggggagccgg taacgctgta tcactgtcca acagcggtcg 183240
ggttcctcgt ccggacatgc gggtttccag caatcctcgg cgtcggcgcg tccgatatag 183300
aagtagttgc gctgaaaacc gcggtacatc ccgcagtcgt gattccgtag acgccagggc 183360
gtcggcgacc agatctggtc tcccagcgag tagcgaccta acgccggcgt gcagcaaggt 183420
tcgtcgggcc ggctgagcgt ctccagttgc gtgagaatta cgaagcgttg catgatgagg 183480
ccgtggctgt agttgcgcag cacgcattcg tacatgccgg ccgtgtccgt cgatacgttg 183540
aaagtcagcg agaatatttg gccgagatgc aattgcgaga aattccaagt ggcgtacggc 183600
aggcggtact ggagtccgtt catcagccga tggcctttga cggcgtccag gatgagctcg 183660
tcgctgccgt cgtgggaacg acagaaacgt gcgcgaatgg agaccatggg ccaggagtgt 183720
gtcatgaccg tgcaggggat ggtaacttgc tctccctcgg cgaccaacac cggcgccggc 183780
gacgtggtct cataattctc ggcccacatc ttttcggcaa tgtcagcggt ggcgaagggg 183840
aacgaagagg aagaatattc gaggagtcgc gggcagctca acagcaccca gaacagccac 183900
ggcagagttc ggagcgactc ccggcggcac atgatgattc tttccttccc tttttcgcag 183960
agacgctgcg cgcctgctcc tgctccgtgt gtcggccgct caaacgtcgg gccggcgtgg 184020
tggtgaccac cgtgcgacgc agcttctcgc ccgggatgcc cgcgactgag cgtccggttt 184080
ttttgcaggt cttttttgct gcctcctcct cgccgtcgcc gtcgcggccg acgtggtgga 184140
ccagcaccgc gcaggaactc tcgcgtcgcc ggcggtacgc gacctgtctc attgctacct 184200
cggatgttta agaaggaacg ttcatctgcg tcacagggtc tgatgaagct gccaagagtc 184260
gtggctgtgg cgcagcgcgt tctgtacggc gcgtttcacc gctttctgca tggccgctac 184320
cacgtcgggc gggagcggct ccggcggaag ctcgatgagc agttgctgcg agtctcggcg 184380
ctcggcgtcc gccgtttcgt cggacgtggc gtagaaaacc gaggtggtcg cccagtcgtc 184440
cacgctgtcg acggcctctg tcagtgccgg gttgtcaaaa ccgccatcgg acgcgggtga 184500
taaaagaacg tacgatgaca cgctgttagt acgattctcg tcgtcgctct gggaacgacg 184560
tgatggacga cggtagatga cctcgtcttg ccacgcgtcg aagcgatcgc agcagcgctg 184620
gatccaagcg cagcgaagca gcttacggaa cacgtcgttg ttccaaaagt agagcataaa 184680
gagaaagaaa agtagcgtaa cgatgaagcc gaaaacgacg agggtcggca gggcactacc 184740
gccgctgccg ttttttgtgt cgtgcgggtg cacggtggta gtggcgttag tctgagctgg 184800
ggtcatgaca agtctgaaga gatgagagcg tgggtgctca tcagggacag ttgaggtctc 184860
tccctaccga agccttagcc tctacggtgt tttatgatca acgtgtctac gaacgtcatt 184920
gtgaaagtga cgtctcaggc tttccgaaac cgcgtcagat tcaacgtggg tttcggttta 184980
gcctgcgtca ccgaggcgga ggtggaaatg agccgtcctg tgggggagtg tacgaccctg 185040
tagtgcccat gggtaacgtc gcgtcggaag aagtgaatgc ggcattggtg tacgcgtggg 185100
ttgttttgct ctctgactcg gaggagttgc cgcagcagct gcagatttta cgtactagcc 185160
aaaagcagca aaagcagcag gtaaataaga gaaggagtcc agataatgtc cagccgctag 185220
cggcaaacag cgcaagttgc gcgactgtcc aattactacc accaaaactc tcaacacatt 185280
gaatcgacgc tgaggttggt gttgcagtgc tgttgctact agtggatgaa gacgaagtag 185340
attgactgga attagagctg gtacctgtag tggtttcact tgccgatgcg gcaagtgcaa 185400
ataaaactaa tatccacagc atgttcgtta ctatataatt gatatacgaa cccgtttgtc 185460
gtaacaatca gcgttatata cgctgtatcg gcatcgtttt accggaaagt ttatcgtaat 185520
gtaacccgcg ttgtgtacat tcgtactgaa agggaacccc cggtgatgtg cacattatac 185580
tctttcattc tggggtttcc caatgacgta aaaatttcca ctatacaata aaattacgga 185640
atcatgtgaa aagtgtgctt tttattaaca gagcagaggg tttacagtag atatatgttt 185700
gccagggcca ctgttttcta acaccgatca ccgccaccat taccacccgt tgaactccac 185760
acccgggagc cgcctgatcg ccagggactc ctcaccgtcc atcgtccgaa caagctcccg 185820
ccaccgatgc tgccaccatc accgagagaa agaaccgctt gctgcagata cgcttgggct 185880
cgcctccgtg cggacgccgt ttcgtgcaga cgctgagtag atcgagcaga gaatgtcaaa 185940
acgacattac cgcgatccgc tcccctcttt tttctttttc tcattcacgt gtattcttga 186000
tgataatgta ccatggctac ggtggtgaac tgcgtcgcgg atcccgtcac gggtttcaac 186060
agatcgacgt cggtcagcgg cgccgtcacc gccatgtccg gcggaggcac gctgtttctc 186120
tggttagcga cgtggaccga cgacgaagac gatgaacccg cgcggcggtc tgttatccgc 186180
gacgacgcgt agctgcactg ggaagacact tcctcccaac ggaccaagat ctcatcgggc 186240
cgttcggaga aacggtatcg tctgtccgac tcccgccgta cggcgccgag gcccagcgac 186300
gacaggtccg cgaaccggcg ctcgtattcc ccgtacagct cgcaacagcg gatcagccag 186360
cggtagctca aaaacatgcg caccagtttg aaggtgtcgt gccaatggta agctagatag 186420
cagagaatgg ccacgatcag cacgagcatc acgccgatga tgggtaaccc gacgttcagc 186480
ggcagatcgt ccatggtgac cgtcctctgt ccggatctac gtcccagtct ctctcttttg 186540
tacagcactc gcgcgggaac ggccccctca accctcttac gtagcgggag atacggcgtt 186600
ctcccgcggg ccacttactt gcacggtcgc ttgaacggcg gcttggactg ccacatgcac 186660
cgcatccatc catttcggca gcagcgcgtt cgacgatgtc gtacgagtcg cggatgatgt 186720
taccccgcca gcacctccgc cggcaaccgc gtcgtcgttg ctatcgtcgc cggtttcggg 186780
cgatgacagc gccggcggcg cgggtctcgt ctcgtccacc atttccaccg tgtcgaagcg 186840
acagccgctg ccgtagtaca tagctccgtt caacggccgg cgggccgggt cgccgagttc 186900
cgggtcgggc acatccatgg ctcgccgtct ccttctttgc cgctcgtggt gccgacggca 186960
cttctcggga taatgacagc cgcaaaatag atcgtggagc atgtctcgcc aactgtcctg 187020
gtgataatat cttaagtacg cgatgagcgc gccgatggcc ataatcataa gcgtaagcaa 187080
aacggcacag ataacgtgaa acaccgcggt catccaagtc gggcggcgtc ggggacgcgg 187140
tgggtcggtt tctcttacgc cggcgtcact cagccaccac acccgtagcc gacattccca 187200
gaatcggtga atgcgactca aggcctttcg acgccgccat ttatttccaa cgtccaagtc 187260
ccacgtcatt tctggcatct ccacgccctt gactgacata ctctctttct ctctcttagc 187320
tgcggtgaaa aagagggaag gcgtgtgctg ctatacaact gtacaacgga cgcgctcgct 187380
ctttcggtct caggtcatct gcatcgactc ggcgtccttc atgacgctct gcaccgcctt 187440
ttccaacagt tcctcgatgt ccgaccatcg aggaggcggg gctaactcgg aaaccgacac 187500
gataggcagc gtggtcggct ccgttggtgt gcggggtcgg ggacagggac acgagagtcc 187560
caccttcgag agattctcca gcccgacggt gcgcggcagt ctcggattcc gcggcggctt 187620
ttgcggcgtc ggcgttttcg ggaagggcct gggcgtcacc ggcggtgtcc agccgaccgg 187680
cttgggtttc gtgggcggcg gtgttttctt ggtgagcggc gtgctcaggt tcttacgcgg 187740
cgcgggtatc ggcgtcgggg gcctgtgcga cgacagccgc gtggtggggg cccggaccgg 187800
cggcgtaggc ggccgcttct tgcgcccggg cggcggaggt ggcttccagg atggtggcgg 187860
ctgatgcagc accgtgtcga cgctggtcga ggacgacaaa gagctcgacg aggaacaatg 187920
cgacggagat cggccgatgc tggttggcgt tcccggagtg gatacgtcgg ggatctcgaa 187980
ccgcgccgga ggaaactcgg gtttatctat cggcagacca tcctctccta tgtagagcga 188040
cgtacaccgc ggcacctgcg gcgtcggcgg gtgggtggcc acccgcatga gccccagttc 188100
cagatccagc ggctcgacga cgtcttcttt cggattgcga tagcagcacg cgcaggcacc 188160
acgcttatca gaagcagcac ccgggagccg gcctcgcgac gaagtctcgt cggatcgctt 188220
gcggcctcgg cgctgggtaa ataaggaaat ggccaggacc agggaagcca gtccggtacc 188280
gccgaggagc ccgacgccga gccacagcca caccatgatc ttctctcctg cttggaatct 188340
caaactccgt gtcgggaagg gccggtgtac ggacatttat gccttggatt tctggaaacg 188400
tcattttttg gcaaggaatg tgtttattgt ccaaacactg aggaaggaga tgtgggccaa 188460
gtcggaaaat tccttatcac accgggggcg ggttacgttc cggtctgatg ctgctgctgt 188520
tgttgtagag ccgcggccac ggccgtctgc acggcagctt gtaccgcctc ggccacgccg 188580
ggtggcatct gcggcatggc ggggggaggc gcgtcgggcg gaccgccggg catcgccgtc 188640
ggctgtgacg gtggttgtga actcaccgtc ggctcgcacg gaggtttgtt cttcggtcta 188700
cccctcggtt tgtctttcgc cctacctttc ttcggtttgg gttccgatgt cggtgttggc 188760
ggctgcggtg ggatgacggg ctggtgggac tcctccgacg gcggggggac gaacaccgtc 188820
ggcgccgaaa ccgggggact ctcgactatc tcgcagatca ccctgtcagg atcgtcgccg 188880
tgcccgggac gccgtcgatg accgtattgg accatgtcgt aaatcatcgt ctccttgtaa 188940
cacgctgaac agcagcggct gcaggggccc gagatgcatt tacagctgca cttacagctg 189000
cagctgcagt agcgcaccca tcggcaagtt aaaatgtcga ttatggaatc tttgaaaaat 189060
tcccggtagc ggatgaggta cgcgcagagg aaaatcatga aaacggaaca gacgaccaca 189120
gccgcgatgc caggtccaga aaaaatattc gctgatgaac ccgccaaaca ccaaattccc 189180
aaggccgcgc atatcatcca gatcacaatg atcgcgggga cgccccattg gcattggcac 189240
gaaggatctt gcacatcgca acccatcgct actgcgttct cccacaaacg ccatcgcact 189300
atttatccct acagcggctg ccgagtcacg tccgccggcg cccatcggcc gcggcgatct 189360
cctagtaaca ctcgtccgac acttccacca tctccagctc ggccggcggt tcggcatcct 189420
ccaccagcgg cgtcgtctca tctttgccgc agcagcggac gcacaccttc tccaggcaga 189480
acgccaccag ctgccgccga acgtaccaca ggtacacgtg cagacctgcg aacaggacta 189540
cggaggtcat gaccaccacg acgcacacgg gaatccaagg atcgagattg tcgctggaac 189600
tcatggctat cgccaccgac gtgcccgcgt ctgtctcacc gccgctcgcc cgatgtcgca 189660
cggcttgtta tacgctagcc cgtcgccgcc tcggggcacg gtgccctcct acccacgtaa 189720
cttcctccgt gacttaaagt cgcgtgtggt agatctcctg ctccgtggac gaaccgttcg 189780
gcaggatagc ggttaaggat tcggtgctaa ggccgtgtcg ccaacgtcga atgctacgtt 189840
gcaatagctt cgacggacgg ccatcctccc tctcatcgca ataataaaac accagcagcg 189900
cacacgacgc gatcacggtg acccccatga ctagacccac gcagatagcc agccccgcta 189960
gcgtatccag cgccatcccg ttcgctcccg tcgtcgtctc ctgaacaaag caactccgca 190020
gtccccgttt tcaaccgttt tcaaccgttt ttgtttcctt ctccgcgact agatgttaac 190080
gcccgcggtc tttccggccg tgctctacct cctggcgctt gtcgtctggg ttgagatgtt 190140
ctgcctcgtc gccgtagccg tcgtcgagcg cgagatcgcc tgggcgctgc tgctgcggat 190200
gctggtcgtt ggcttgatgg tggaagtcgg cgccgccgcc gcttggacct tcgtgcgttg 190260
cctcgcctac cagcgctcct ttcccgtgct tacagccttc ccctgaaacc cgcgtcaatc 190320
gactgtcccg aaaacgccgg cgttaacaca ggaaaaaaaa accacgcagg aaccgcgtag 190380
gaaccacgcg gaacatggga cactatctgg aaatcctgtt gaacgtcatc gtcttcactc 190440
tgctgctcgg cgtcatggtc agcatcgccg cctggtactt cacgtgaacc accgtcgtcc 190500
cggtttaaaa accatcatcg acggccgtta taaagccacc cggacacgtg ccgcggcact 190560
tgcctacggc gctgctccag ggaaactcct cttccttctg ctcttcctcc ttcaccgcag 190620
ggaccgtctc cctcgaccag ggacccgccg aagcaaccgc cggaacaacc tggaggaggc 190680
gcggcatgac ggcacccaag tgtgttacga ccactactta tctggtcaag accaaggaac 190740
agccctggtg gcccgacaac gccatcagga gatggtggat cagcgttgcc atcgtcatct 190800
tcatcggagt ctgtctggtg gccctgatgt actttacgca gcagcaggca cgcaacggga 190860
gcggcagcgg ctagataagt ctctggcggc tacagctcca agcgccgtag ccggcccgcc 190920
tgccgatcgc gacgtcgtgg accatcgaac agagactcac gcgtacgaga ccccgaggta 190980
cgccacgcgg tgcctaacgc ggtataccac acccgtacgg tctgcagtgc ggcgtacaac 191040
gtgtggaaaa cgcgttgcgt cgcagagtca gccacgtccc cgtcttgtcg ctccccaatc 191100
ggctcccgca caccccccgc ggcacccaga gggcgggtga gccaagtatt cttaaggccg 191160
ttctctgttc catatcccat aaattgttta ttccggagct cgttggcgcg gaaatagccg 191220
gataagggga gcaacaaccg tcggcgaaag ccgtcccgct cattcagtcc gggtttcgcg 191280
tctagtcgga ggtgtgaccg ttggccaacg gaacggcgtt tcacgatcaa aatcgtatcg 191340
ggtagtgtag gagacgtcgg cggtgcagaa tgcgactcgc ggcgtagctc gccgtcgcta 191400
tgcggctcgt cgccgtgtgg cgcggcctgg ccggctgtct gcgcccagat ctgttggcct 191460
tttgggtcct ctggctgctg ctgcgtgtgt gctttggcag acgcggtggc aatttgcggt 191520
ctgcggtaag tgaggatatc gccgagcaag cgcatttgcg gcacgtgggc ggcacgcgtg 191580
ttattgttcg ttcgttgcca gatagcaagt gctgtcgaca gcagacgttg tgggcggttg 191640
gtgtattttt gcgggttgcg gtgaaagtcg gcagccggcg tcttgtgaag tatcttaacc 191700
atctgtgttg ctttttgcag cgtccagaaa agcgacgcga ctttggggat ggcctcgtgc 191760
tcaccttcgc ggagagcgcc gccggacctg ctcgtcagca gcgagctacg cagacggaat 191820
atctggagga gagttacgtg tgtcacaggg gagcgcgggt ctccggcggt aacgacggcg 191880
gtatcgtcga cacgtgtgcg gcctgctgtg ctctgcggaa aagcgccggt ctcggagacc 191940
gtggacgaaa aagagaacgc agcagctacc gctggcggcg gcgtcgttaa tgctgccgtt 192000
gatgatcgac gttgtgagta ctcggaaaca gcggtgaggc agaagctcgg ttctccaggg 192060
aacgaccgtc gatgcgtggt aggcgcagca ggtgaggttg gggcggacaa cgtgttgcgg 192120
atcgtggcga gaacgtcgtc ctccccttct tcaccgcccc acccaccctc ggttggtgtt 192180
tctttttcct tgtgttctgc agatagttcc acggacagcg acggcaagtc cataagcacc 192240
ggtgtgcaag tggtggagca cgacgaagat atcaccgcgc cgcagagttt gtggtgcacg 192300
gcgttcaagg aagccctgtg ggatgtggcc ctgctggaag tgccgcgttg ggtgtggcag 192360
ggctggaaga ggtggcgcaa cagcgagtcc gggcgtcgat ggagtgctgg gtctgcgtcg 192420
gcttccagct tgtctgactt ggcgggcgag gccgtgggag aattggtggg ctcgctcgtc 192480
gcgtacgtca tcctcgaacg gctgtggttg gcggcccgag gctgggtgtg cgaaacgggt 192540
gtgcaagccg aggaggccat ggcgcgacgg cgacagcgca tgctgtggcg gatgttctct 192600
cgtggaggcg acggcgaatg cagcacacgg tgtgcgatgg agatggcgtg cgaggaagaa 192660
agcgccgtgt tgtgagccga cggcgcggga cgcgggccgg cgcagcgcgt gggccacgtg 192720
tggtggcagg cggcgtcgtc cgcttgcggc cgtcgccgcg ccgcacagac gcaaacacat 192780
gtcgccgtca agagaaacag tctgagcata gccgtctgca gcggtccgcg tgtagaagcg 192840
gggggagaac gacgttaata aagaatagcg gcggtgccga tagggcgacc gctgaagcga 192900
gctgcgtgtg cgtgcctgtt ttgttccccg tcgccgccga aaagctacgc gcggcccccg 192960
tccctagcct tgagcgcgcc acagcacgcc gcaaactcgg cgtcgcgctt acgtcccgca 193020
aacccccctc agcctcgtcc cgccaaccaa taccgtggca cgcagtgcca tagcgccgcg 193080
cgtcaaggcg cttacacccc cctcagcccg gtcccgcacc ggcgtcggtc tgggtgtggc 193140
gggggtgcgg ctgggtgggt gtgtgccggg tgcggctggg tgtggcgggt gtgtcgcggg 193200
tgtgtcggct ggctgtgtgg cgggcgcgtg ccgggtgtgt cgcgggcgtg tgccgggtgt 193260
gtcgcgggtg tgtcaggggt gtgtcggcgg ggtgtgcgcg cggccagatg gaagcagtgt 193320
gccccggggc ccgcgatccc cccccccgcc ccggcgcggg cgcttcttct gcgtgtgtcc 193380
tcgacgcggg tctgtgcgcc tgcctgccgg tcccggcaga ctgggctgcg gcttcctcgt 193440
tttttttttc cgcctgtggc cgtccccggg gacttcctct tttccgcgtc cgatcttcgc 193500
gtccccaggg agtcgcgccg ccgtcccctc gggaccgctt cctcttttcc ccggggactc 193560
aaagacacgc aagacagacg cgcgactgaa agagacgcaa gacacacgcg cgtctgggtt 193620
tcgccgtgcg cgccgcacgg cgcttttatt cgccgtcgcc gtcccccgcc accgccaact 193680
tcccaaattc ccacatttca cccccccgat gaaaacaccc ccccgcccct cggggaccca 193740
gcacacggcc cggaatggag gtcaggcgtc cacctaggtg tgcgcgcgct cggcggcccg 193800
ttgttggtgg cttgtcgcgc atcttctttc ggttttttca cggccttcca gactgcgcgg 193860
cggcaaggcg gcgccagcaa gcgccgtgca cgtcgctgcc tataaaagcc aggtgcgtgt 193920
cgcccgcggc acacgggcga cggaggcgtc cgcgtgtgta aacggcgtgg tcgctgacgc 193980
gggtttgctt cctatatata cagagtggac gtcggaggcg tccggcggcc atggcccagc 194040
gcaacggcat gtcgccgcgc cccccgcccc tcggtcgcgg ccgcggagcc ggagggcctt 194100
cgggggttgg ttcctctcgt tcttcttctt tggaagcgac gtcaacagcg gggactagta 194160
cgagtactgc gggtacggcg acgccggccc acgccgtcca ccgggtagaa ccccgcgggc 194220
cgccgggcgc ccctccgggt agcggcaaca acagcacctt ttggcacggc ccggagcgct 194280
tgctgctgtc tcagattccg gtggagcgcc aggcgctgac ggagctggaa taccaggcca 194340
tgggcgccgt gtggcgcgcc gcgtttttgg ccaacagcac gggccgcgcc atgcgcaagt 194400
ggtcgcagcg cgacgcgggc acgctgctgc cgctcggacg gccgtacgga ttctacgcgc 194460
gagtgacgcc gcgcagccag atgaacggcg tgggcgccac ggacctgcgt cagctgtcgc 194520
cgcgggacgc gtggatcgtg ctggtggcga ccgtggtgca cgaggtggac cccgcggccg 194580
acccgacggt gggcgacaag gccggccatc ccgagggtct gtgcgcgcag gacggactgt 194640
acctggcgct gggcgccggg ttccgcgtgt tcgtgtacga cctggcgaac aacacgctga 194700
tcctggcggc gcgcgacgcg gacgagtggt ttcggcacgg cgcgggcgag gtggtgcgcc 194760
tgtaccgctg caaccggctg ggcgtgggca ccccgcgcgc gacgctgctg cctcagccgg 194820
cgcttcgcca gacgttgctg cgcgccgagg aggcgacggc gctcggacgg gagctgcgcc 194880
ggcggtgggc cggcacgacg gtggcgctgc agacgccggg caggcgactg cagccgatgg 194940
tgctgctggg cgcgtggcag gagctggcgc agtacgagcc gttcgcgtcg gcgccgcacc 195000
ccgcgtcgct gctgacggcc gtgcgtcggc acctgaacca gcgtctgtgc tgcggctggc 195060
tggcgctggg cgcggtgctg ccgtcgcggt ggctgcgctg cgcggcaggg ccggcgacag 195120
ggacgacggc ggggacgacg acgacgatga cggcggggac gacggcgatg gcgacgggga 195180
cgacgttgct gccgggggcg agcggcacgg agacggaggc cgccggcggg gacgcgccgt 195240
gcgcgatggc gggagccgtg gggtctgctg tgactttacc tccgcagccg tacggcgccg 195300
ccggcgggag cgcgatttgc gtgccaaacg cggacgcgca cgcggtggtc ggaacggatg 195360
cggcagcggc agcagcggcg gcgccgacgg tgatggtggg tccgacggcg atggcgggtc 195420
cggcggcgtc ggggaccgtg ccgcgcgcca tgctggtggt ggtgctggac gagctgggcg 195480
ccgtgttcgg gtactgcccg ctggacgggc acgtgtaccc gctggcggcg gagctgtcgc 195540
actttctgcg cgcgggcgtg ttgggcgcgc tggcgctggg gcgcgagtcg gcgcccgccg 195600
ccgaggccgc gcggcggctg ctgcccgagc tggaccgcga gcagtgggag cggccgcgct 195660
gggacgcgct gcacctgcac ccgcgcgccg cgctgtgggc gcgcgagccg cacgggcagt 195720
gggagttcat gtttcgcgaa caacgcggtg accccataaa tgatcccgtc gcatttcgtc 195780
tttcggacgc tcgaactctc ggtctcgacc tcaccaccgt catgacagag cgtcaaagtc 195840
aattgcccga aaagtatatc ggtttctatc agattaggaa acctccttgg ctcatggaac 195900
aacctccacc cccatctcgc caaaccaaac cggacgctgc aactctgccc ccaccgctca 195960
gtgctcaggc aagcgtcagc cacgcactcc gatacgatga cgagttgtgg cgcccgctca 196020
gtacagttca cgaccacaaa gcctggttgg atctcgacga atcacactgg gtcctcggag 196080
acagccgacc cgacgatata aggcaacgca gactgctgaa ggccactcaa cgacgaggcg 196140
ccgaaatcga cagacccatg cctgtcgtgc ccgaagaatg ttacgaccaa cggttcacta 196200
ccgaaggcca ccaggtcatc ccgttgtgcg cgtccgaacc cgaggatgac gacgaagatc 196260
ctacctacga cgaattgccg tcgcgcccac cccagaaaca taagccgcca gacaaacctc 196320
cgcgcttatg caaaactggc cccggcccac ctccgctgcc gccaaagcaa cggcacggtt 196380
ccaccgacgg aaaagtttct gcgccccgac agtcggagca tcataaaaga cagacccgac 196440
cgccaaggcc gccaccgccc aaattcgggg atagaaccgc ggcccatctc tcgcaaaata 196500
tgcgagacat gtacctcgat atgtgtacat cttcggccca caggccacgg ccgccagcac 196560
ctccgcggcc gaaaaaatgt caaacacacg cccctcacca cgttcatcat tgaaagtctc 196620
tccagtccat atgttgtcag gacgtgctgt cgttctccgc ttgctgcgaa gcccgttctt 196680
ccgagtcgtg tcgctgcgtc cagcgtcgcg cccaagatgg gaatttgggt ctcttcacgc 196740
gtagcctcct ccaccacggc tgctgatcgc cgtcactaag gaccgacacg gaggatgacg 196800
aggagcttct ccccgactcc gcggtccgcg accggctacg tagcgcgtgt ccctgccagt 196860
ctccgcagtt acaccacacg tcgtgagcag cgtgcacctg ctgccgccac tgggcctcgg 196920
cgtgctcggg ccacccgccg gagcccggtc tgagctccga cgcaggatgc gcgtactcaa 196980
cgtgcgcctt ccagtccata cagcaacacc ataggtcgtg cgagtcgtcg gctacccgcc 197040
gccaggccag ttcccgcatg ggaaggctgg acacgccgac cgagaggtca ccgagcccgg 197100
acgccatctc ttcttcctct ccgtcgctgt cattaagcag ccaggtcacc tcctccgctc 197160
cgcggtccgc cggtctcgac ggaccgcgcc gccgtcggca acacggaaaa cagtacgcca 197220
gcccgagccg ctaaggccgc atgcccctgc cgcccaactg aacacgcata tcccgctcaa 197280
ctgcgttttg ccacccctgc cagtgctccc gctcgagcac caccccgcat ctcccaacct 197340
ttttccaata aacgaaaccg acatgacaca cgtaatgggt actcgtggct agatttattg 197400
aaataaaccg cgatcccggg cgtctcagca cacgaaaaac cgcatccaca tcatagacaa 197460
gttacagtcc acagtcacat acacgataaa caataccaac agggtaatgt ttatggagta 197520
aaacactatt gtccaggcca catgcgtgta tgacttccgc accatcccgt actgcatgtt 197580
ccacatgtac gcgctagacg tgtaatccac tcgcagttcg gggacgcaac gcagccagat 197640
cacatcccct tgcagtacca gacgcagggc tagcgtctcg aagatcggca tcacatctaa 197700
gttccgcacg ttccacttta acgactcccc gggaacgaac tccacgtcgt cggcgtgtac 197760
gtacaggttc tctcccacgc cgccataatc ggccttcgga tcgaagacga accgactcat 197820
gttgcccacg atgctccccc gagcaaacaa cttgccgttg tcaatgtagc accggttgtc 197880
ctcgatttga aaccagggat gcttggccgt ggacttccag ggccggagcg cgtcttcccc 197940
ggctttagtg attccatcgg gcaggcggat caagggaccc atggaggtcc aaagacccac 198000
ccaggctttc cagagattgt tcatggtgaa acagcgtgtg gactgtacgc tctttcccaa 198060
tttatatccc agagtagtga cgtgagccca gccacctccc agattcctga cgttttggtt 198120
gtctttcctg ccaattcctc ccgtaaactt atgattatcc tagcccattc ccgataaaaa 198180
tacacggaga cagtagatag agttacgaat aaaccggttt atttattcaa gtgtctcagg 198240
agattattga acgagcgtgg ataccacgcc gtcgtcagtt catggtggca ttgagcagcc 198300
atagcaccag agtcccggcg cccggtatca gacacgctga cctaccgggc gccttcgagt 198360
ccgtaccccg cggcctgggt gttagagtcc gtaccttgca gcccaggtag gtttcaggta 198420
ccagctggtt cgtacctgtt aaataaatcg cagacgggcg ctcaccccta cggtcaggag 198480
cacaagaaca accagagaga acagatatac gagcagggtt ctgaacagca gaccccaatt 198540
gtcgtctctc atgcttcgct gaaggtacca gttgatggtc tgagagctat agtccatcct 198600
cacctgagga acacacgcgg catatttctt ggggtctccc cacctcgtag acaacgtgat 198660
gtccaccata tccacggtgt gcgtcaccgg gtgcccaccg atgttccact cgaaataggc 198720
tccgcgctca tcatggtggt actgctcacc ggacacctgc agtctgtcca tgtaagattg 198780
agagacgata cccacgttca caaagtgttt ctcggtgaag ttgcccgaca tcctcccctt 198840
gaagtacagc atgcccatat ggaaccagca ttggttctcc tccactcgaa agtgggccga 198900
tctgatctcc gataccacca catccagggg ccggggcacc gagtccgcga gtctcaggaa 198960
caagacggcc aggatcgcga gcaccaacac cggcttcatg gctccgaagg tccgctgctc 199020
ggctccgctc accgctccgg tctggctgca gcagtgcttc gctgagaagt agcgtgtgga 199080
ctgaacggtg tttttgaata tatagcgttt cttggtgacg ttgtttcccc tacgtagtag 199140
gcaactacgt gccaaaagag gcgttacggt actttccgta ctgggatttc caaaccggga 199200
ctttccacac ggcggtttca acaccgggac ttttcacacg gtgatttcgg caccgggact 199260
ttccgcacgg cggtttcgcc accgctgacg ttctcatcgc cgcccacgtc aacggtggcg 199320
acaccgtact ttcccatgcg gtttataaac gtcaagagtc acgtcagtcg cccaccccca 199380
ttacacggcg atatcccgat agggcatgag gggacccggg tgtcgcgaca tgtcgacgac 199440
aggtgcggat tagtggtcgt gtcgcgacat ggacgtgcag ggggatgtct gtcgcgatag 199500
agttgatgtg acagcccgct acacctctct gtcgcgacat gcatacacaa cgggccggct 199560
tgtcggcgat tgtcgcgaca tatcgttatc agttagcgac cggagttgtc tatcgcgaca 199620
tatcgtcgac tatcgcgaca gaaaaaatac cgttcgtaga gaatgccgtg ttgaaggaac 199680
gcgcttttat tgagacgata aaacagcatc aggagccaca acgtcgaatc ccacgtccag 199740
tcgattcgta tgttatgctg cacagcaatg ctagaataac aaccagcagg gtaatcccgc 199800
aacataaata caaagtcaca gcgaagaatc cgtgtcgttc tatcaagcga aacgcgttcc 199860
aaacggcccc gtcacagacg cagttattca taagcgttaa caaccggtgg ctaggatgaa 199920
tatccaaatc acagggcagt agccgacgga ctcgttgaca ggtcagccta ccctcaaggt 199980
tcctatcgtt cggacgggat ttgtgcgttt taggcctctt tttcgccgcc tgcaagcatt 200040
ggtgcgcaaa gtcctcaccc agctgtttcc agctatcatc tgcatctgtg cagtcccctg 200100
tatcgttgta acaaacgggt ctgtgcgact tcgttctcgg aacacaagct tgttgtcgcg 200160
gagacagaga gagaagggtt ttcgggtcac gcgaagaccg ctcaccgggg gtcggcaacg 200220
cacacatcaa cagaaaaccg agacgaatca agagatccat agtgaaggag tgatatcgac 200280
gtgcttacga aacggcgatt atatatgttc tcaacaatac cgccctacgt tgtatgatgt 200340
aacgtgtgac gtgagtctga tccaacactg aacgctttcg tcgtgttttt catgcagctt 200400
ttacagacca tgacaagcct gacgagagcg ttcatcgggg catgaagtac gcattacaca 200460
aactccatat atttgttacg atagaatacg gaacggagga ggctttcgcc acacctatcc 200520
tgaaagcgtt gcattcttta tgataggtgt gacgatgtct ttaccattcc cacggctgct 200580
ttgcgtgatg atgacattca tcatgtattt ccattcacac ataccttttg tgcatacggt 200640
ttatatatga ccatccacgc ttataacgaa cctaacagtt tattagccct tgacaggata 200700
ggtcaaaaga ttatatgtag gttttccggt aaaccgaatt gtgatatttc tctgcaggaa 200760
atagaacagc ctggtaccta taaaacggac aatgcagtac tgtagcagcg taaccaagta 200820
ggtccacatg aacacgtaca aaattatggt aagccatcgt ttttcatacc acagcctgta 200880
gctgtcgtac atgaatgagg acggtcgagg aacccagggt agttgtaatt gggggcgaca 200940
ttcgtactgt ccagaagaca attgcacggg tttcagtgag atgagtactt tagcgatgtc 201000
ggcgggggcg ctacgtttca ccgtgacggt gagaacttga ccgtcgtttt gtatttcatg 201060
aggcacgtta tacaagccac tggtatcatg aaggatgacc tctgatgcga tgtgaggatt 201120
aaattgtccc tcaaaccgcc aaacgctggt catgtttcca ccgtcaatta cgcagctgac 201180
ggtgtgagat accacgatgt tggacttagg tttgggggct aattgccttt ttacaaattc 201240
ccttctgtat tgcaggtcct gctgccactg cttttccgtg cggaaagtcg ccatgtcttc 201300
cacacgtgtg gcgacgatag acgccaccaa ggtagctacc agaagcagct ggatccgcat 201360
ggcattaccg tatgtcaatt agaaagttga gcggacacgg ttatcgttcc tggcggatat 201420
aagtatataa acgcgagtta gcctttcccg tccgttttgt acacccgttc cccacacaaa 201480
tgacgaatac gacctttttt tttataaaaa taaaccacgt gtattatata aaaacattta 201540
catagaaaag agacacactc tagattaatt aagggccggc cgcatcagct tgatatcgaa 201600
ttcctgcaga tctgctagat aacttcgtat aatgtatgct atacgaagtt atgcggccac 201660
ggatgcatgt ttaaactcga cagcgacaca cttgcatcgg atgcagcccg gttaacgtgc 201720
cggcacggcc tgggtaacca ggtattttgt ccacataacc gtgcgcaaaa tgttgtggat 201780
aagcaggaca cagcagcaat ccacagcagg catacaaccg cacaccgagg ttactccgtt 201840
ctacaggtta cgacgacatg tcaatacttg cccttgacag gcattgatgg aatcgtagtc 201900
tcacgctgat agtctgatcg acaatacaag tgggaccgtg gtcccagacc gataatcaga 201960
ccgacaacac gagtgggatc gtggtcccag actaataatc agaccgacga tacgagtggg 202020
accgtggtcc cagactaata atcagaccga cgatacgagt gggaccgtgg ttccagacta 202080
ataatcagac cgacgatacg agtgggaccg tggtcccaga ctaataatca gaccgacgat 202140
acgagtggga ccatggtccc agactaataa tcagaccgac gatacgagtg ggaccgtggt 202200
cccagtctga ttatcagacc gacgatacga gtgggaccgt ggtcccagac taataatcag 202260
accgacgata cgagtgggac cgtggtccca gactaataat cagaccgacg atacgagtgg 202320
gaccgtggtc ccagtctgat tatcagaccg acgatacaag tggaacagtg ggcccagaga 202380
gaatattcag gccagttatg ctttctggcc tgtaacaaag gacattaagt aaagacagat 202440
aaacgtagac taaaacgtgg tcgcatcagg gtgctggctt ttcaagttcc ttaagaatgg 202500
cctcaatttt ctctatacac tcagttggaa cacgagacct gtccaggtta agcaccattt 202560
tatcgccctt atacaatact gtcgctccag gagcaaactg atgtcgtgag cttaaactag 202620
ttcttgatgc agatgacgtt ttaagcacag aagttaaaag agtgataact tcttcagctt 202680
caaatatcac cccagctttt ttctgctcat gaaggttaga tgcctgctgc ttaagtaatt 202740
cctctttatc tgtaaaggct ttttgaagtg catcacctga ccgggcagat agttcaccgg 202800
ggtgagaaaa aagagcaaca actgatttag gcaatttggc ggtgttgata cagcgggtaa 202860
taatcttacg tgaaatattt tccgcatcag ccagcgcaga aatatttcca gcaaattcat 202920
tctgcaatcg gcttgcataa cgctgaccac gttcataagc acttgttggg cgataatcgt 202980
tacccaatct ggataatgca gccatctgct catcatccag ctcgccaacc agaacacgat 203040
aatcactttc ggtaagtgca gcagctttac gacggcgact cccatcggca atttctatga 203100
caccagatac tcttcgaccg aacgccggtg tctgttgacc agtcagtaga aaagaaggga 203160
tgagatcatc cagtgcgtcc tcagtaagca gctcctggtc acgttcatta cctgaccata 203220
cccgagaggt cttctcaaca ctatcacccc ggagcacttc aagagtaaac ttcacatccc 203280
gaccacatac aggcaaagta atggcattac cgcgagccat tactcctacg cgcgcaatta 203340
acgaatccac catcggggca gctggtgtcg ataacgaagt atcttcaacc ggttgagtat 203400
tgagcgtatg ttttggaata acaggcgcac gcttcattat ctaatctccc agcgtggttt 203460
aatcagacga tcgaaaattt cattgcagac aggttcccaa atagaaagag catttctcca 203520
ggcaccagtt gaagagcgtt gatcaatggc ctgttcaaaa acagttctca tccggatctg 203580
acctttacca acttcatccg tttcacgtac aacatttttt agaaccatgc ttccccaggc 203640
atcccgaatt tgctcctcca tccacgggga ctgagagcca ttactattgc tgtatttggt 203700
aagcaaaata cgtacatcag gctcgaaccc tttaagatca acgttcttga gcagatcacg 203760
aagcatatcg aaaaactgca gtgcggaggt gtagtcaaac aactcagcag gcgtgggaac 203820
aatcagcaca tcagcagcac atacgacatt aatcgtgccg atacccaggt taggcgcgct 203880
gtcaataact atgacatcat agtcatgagc aacagtttca atggccagtc ggagcatcag 203940
gtgtggatcg gtgggcagtt taccttcatc aaatttgccc attaactcag tttcaatacg 204000
gtgcagagcc agacaggaag gaataatgtc aagccccggc cagcaagtgg gctttattgc 204060
ataagtgaca tcgtcctttt ccccaagata gaaaggcagg agagtgtctt ctgcatgaat 204120
atgaagatct ggtacccatc cgtgatacat tgaggctgtt ccctgggggt cgttaccttc 204180
cacgagcaaa acacgtagcc ccttcagagc cagatcctga gcaagatgaa cagaaactga 204240
ggttttgtaa acgccacctt tatgggcagc aaccccgatc accggtggaa atacgtcttc 204300
agcacgtcgc aatcgcgtac caaacacatc acgcatatga ttaatttgtt caattgtata 204360
accaacacgt tgctcaaccc gtcctcgaat ttccatatcc gggtgcggta gtcgccctgc 204420
tttctcggca tctctgatag cctgagaaga aaccccaact aaatccgctg cttcacctat 204480
tctccagcgc cgggttattt tcctcgcttc cgggctgtca tcattaaact gtgcaatggc 204540
gatagccttc gtcatttcat gaccagcgtt tatgcactgg ttaagtgttt ccatgagttt 204600
cattctgaac atcctttaat cattgctttg cgttttttta ttaaatcttg caatttactg 204660
caaagcaaca acaaaatcgc aaagtcatca aaaaaccgca aagttgttta aaataagagc 204720
aacactacaa aaggagataa gaagagcaca tacctcagtc acttattatc actagcgctc 204780
gccgcagccg tgtaaccgag catagcgagc gaactggcga ggaagcaaag aagaactgtt 204840
ctgtcagata gctcttacgc tcagcgcaag aagaaatatc caccgtggga aaaactccag 204900
gtagaggtac acacgcggat agccaattca gagtaataaa ctgtgataat caaccctcat 204960
caatgatgac gaactaaccc ccgatatcag gtcacatgac gaagggaaag agaaggaaat 205020
caactgtgac aaactgccct caaatttggc ttccttaaaa attacagttc aaaaagtatg 205080
agaaaatcca tgcaggctga aggaaacagc aaaactgtga caaattaccc tcagtaggtc 205140
agaacaaatg tgacgaacca ccctcaaatc tgtgacagat aaccctcaga ctatcctgtc 205200
gtcatggaag tgatatcgcg gaaggaaaat acgatatgag tcgtctggcg gcctttcttt 205260
ttctcaatgt atgagaggcg cattggagtt ctgctgttga tctcattaac acagacctgc 205320
aggaagcggc ggcggaagtc aggcatacgc tggtaacttt gaggcagctg gtaacgctct 205380
atgatccagt cgattttcag agagacgatg cctgagccat ccggcttacg atactgacac 205440
agggattcgt ataaacgcat gggcatacgg attggtgatt tctttttgtt tcactaagcc 205500
gaaactgcgt aaaccggttc tgtaaccccg ataaagaagg gaaatgagat atgggttgat 205560
atgtacactg taaagccctc tggatggact gtgcgcacgt ttgataaacc aaggaaaaga 205620
ttcatagcct ttttcatcgc cggcatcctc ttcagggcga taaaaaacca cttccttccc 205680
cgcgaaactc ttcaatgcct gccgtatatc cttactggct tccgcagagg tcaatccgaa 205740
tatttcagca tatttagcaa catggatctc gcagataccg tcatgttcct gtagggtgcc 205800
atcagatttt ctgatctggt caacgaacag atacagcata cgtttttgat cccgggagag 205860
actatatgcc gcctcagtga ggtcgtttga ctggacgatt cgcgggctat ttttacgttt 205920
cttgtgattg ataaccgctg tttccgccat gacagatcca tgtgaagtgt gacaagtttt 205980
tagattgtca cactaaataa aaaagagtca ataagcaggg ataactttgt gaaaaaacag 206040
cttcttctga gggcaatttg tcacagggtt aagggcaatt tgtcacagac aggactgtca 206100
tttgagggtg atttgtcaca ctgaaagggc aatttgtcac aacaccttct ctagaaccag 206160
catggataaa ggcctacaag gcgctctaaa aaagaagatc taaaaactat aaaaaaaata 206220
attataaaaa tatccccgtg gataagtgga taaccccaag ggaagttttt tcaggcatcg 206280
tgtgtaagca gaatatataa gtgctgttcc ctggtgcttc ctcgctcact cgagggcttc 206340
gccctgtcgc tcgactgcgg cgagcactac tggctgtaaa aggacagacc acatcatggt 206400
tctgtgttca ttaggttgtt ctgtccattg ctgacataat ccgctccact tcaacgtaac 206460
accgcacgaa gatttctatt gttcctgaag gcatattcaa atcgttttcg ttaccgcttg 206520
caggcatcat gacagaacac tacttcctat aaacgctaca caggctcctg agattaataa 206580
tgcggatctc tacgataatg ggagattttc ccgactgttt cgttcgcttc tcagtggata 206640
acagccagct tctctgttta acagacaaaa acagcatatc cactcagttc cacatttcca 206700
tataaaggcc aaggcattta ttctcaggat aattgtttca gcatcgcaac cgcatcagac 206760
tccggcatcg caaactgcac ccggtgccgg gcagccacat ccagcgcaaa aaccttcgtg 206820
tagacttccg ttgaactgat ggacttatgt cccatcaggc tttgcagaac tttcagcggt 206880
ataccggcat acagcatgtg catcgcatag gaatggcgga acgtatgtgg tgtgaccgga 206940
acagagaacg tcacaccgtc agcagcagcg gcggcaaccg cctccccaat ccaggtcctg 207000
accgttctgt ccgtcacttc ccagatccgc gctttctctg tccttcctgt gcgacggtta 207060
cgccgctcca tgagcttatc gcgaataaat acctgtgacg gaagatcact tcgcagaata 207120
aataaatcct ggtgtccctg ttgataccgg gaagccctgg gccaactttt ggcgaaaatg 207180
agacgttgat cggcacgtaa gaggttccaa ctttcaccat aatgaaataa gatcactacc 207240
gggcgtattt tttgagttat cgagattttc aggagctaag gaagctaaaa tggagaaaaa 207300
aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc 207360
atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt 207420
tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc 207480
ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg agctggtgat 207540
atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc 207600
gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt 207660
ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt 207720
cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga 207780
caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct 207840
gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat 207900
gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaat ttttttaagg 207960
cagttattgg tgcccttaaa cgcctggttg ctacgcctga ataagtgata ataagcggat 208020
gaatggcaga aattcgatga taagctgtca aacatgagaa ttggtcgacg gcccgggtcg 208080
acagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca 208140
tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat 208200
gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc 208260
gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat 208320
ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggatcgat 208380
ccaccggtct cgaggaagat gtccaattta ctgaccgtac accaaaattt gcctgcatta 208440
ccggtcgatg caacgagtga tgaggttcgc aagaacctga tggacatgtt cagggatcgc 208500
caggcgtttt ctgagcatac ctggaaaatg cttctgtccg tttgccggtc gtgggcggca 208560
tggtgcaagt tgaataaccg gaaatggttt cccgcagaac ctgaagatgt tcgcgattat 208620
cttctatatc ttcaggcgcg cggtctggca gtaaaaacta tccagcaaca tttgggccag 208680
ctaaacatgc ttcatcgtcg gtccgggctg ccacgaccaa gtgacagcaa tgctgtttca 208740
ctggttatgc ggcggatccg aaaagaaaac gttgatgccg gtgaacgtgc aaaacaggct 208800
ctagcgttcg aacgcactga tttcgaccag gttcgttcac tcatggaaaa tagcgatcgc 208860
tgccaggata tacgtaatct ggcatttctg gggattgctt ataacaccct gttacgtata 208920
gccgaaattg ccaggatcag ggttaaagat gtaagtatca aggttacaag acaggtttaa 208980
ggagaccaat agaaactggg cttgtcgaga cagagaagac tcttgcgttt ctgataggca 209040
cctattggtc ttactgacat ccactttgcc tttctctcca cagatctcac gtactgacgg 209100
tgggagaatg ttaatccata ttggcagaac gaaaacgctg gttagcaccg caggtgtaga 209160
gaaggcactt agcctggggg taactaaact ggtcgagcga tggatttccg tctctggtgt 209220
agctgatgat ccgaataact acctgttttg ccgggtcaga aaaaatggtg ttgccgcgcc 209280
atctgccacc agccagctat caactcgcgc cctggaaggg atttttgaag caactcatcg 209340
attgatttac ggcgctaagg atgactctgg tcagagatac ctggcctggt ctggacacag 209400
tgcccgtgtc ggagccgcgc gagatatggc ccgcgctgga gtttcaatac cggagatcat 209460
gcaagctggt ggctggacca atgtaaatat tgtcatgaac tatatccgta acctggatag 209520
tgaaacaggg gcaatggtgc gcctgctgga agatggcgat tagcggccgc gactctagat 209580
cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct 209640
ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc 209700
ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc 209760
actgcattct agttgtggtt tgtccaaact catcaatgta tcttaagctt ataacttcgt 209820
ataatgtatg ctatacgaag ttatggatca acataaggac ttttcacact tttggggtac 209880
acaggcgtgc caccgcagat aataagcgct ggatacacgg tacacagtcc tggccagcac 209940
gtatcccaac agcagcacca tcgccatctg tatggcgatc acgaccccga gctctaagtg 210000
tctgtattca tagtgtagtc gtcgcaggtt atccactgaa ttcccgtagc tgaaataacg 210060
tatatggtac cgaggctggc accacatggg tttgcatttg gagcacggca ccaaatgcag 210120
agtgagatgg tccaagtccg tgggcaccca ctggcgcaaa cggaatacgg cttcggtggt 210180
ctccacgagg cactccgggg cttgcagacg gccccacttt cgtccgtgac ggcccgacca 210240
gccgacccga gccactatcc ctttctcggg atagaacgta ccctgtacac gccatacagc 210300
gtccaacacg ccgtctttga cgacgcagct ggcctgatag ctggacacgt tgttaagcgg 210360
cggaaagcga aactgacgtg ccggcggagc cacatagttc ggttcaccgt gttgtcgcgg 210420
ttcgtcctcc ctatagtaat agtagtcgtc gtcctcatag gggttgccgg cgtgagccag 210480
cgttacccaa cagcagccca ggccgacgag gaggcgcagc caccgcctca tggcggcttc 210540
gccagtcaat cgtctttagc ctcttcttcc cgtgaggtcc ttccggtggc gcggtgccga 210600
cctcggaccc agggacgtat ccacctcagg tacacacagt aggctacctg gacaccgaag 210660
ctgaacaagg ctacatgttt cacaaactgc accagtacca catagaggaa tgtcaggtag 210720
cgtctctccg caaacagccg ttccaagtct gagggcgtta cccgcagcgg caaccagggc 210780
agcctggacg ccggccggca atggagcacg ctccggttac aggcactgca ggggtaaacg 210840
gttaacatca cgtaagagag tcgagcgtcc acctgtggga gctcagtttc gtaacgtaga 210900
gccccgtcat tttccagctg gggtgcgccg accttgaaat gggtcgcgct ccgttcgtta 210960
ccccaggtgc cgtaggctct cggggccgta tcggagaagt tgccgtgcac aagccaggcg 211020
gccacgagta ccccgtgctg gacgtaacat tcggacacgg aactggagac acggtagccg 211080
gacacgtccc caaacccgcg agggtactgg ggcagacgga cggacttgct atttgacaac 211140
ggacagatac gagacgacga ggacgcagac gactcgtcgc tggaccacga caaccggagc 211200
gactccttgg agcggctcga gagtacactt actgcgatca gacaccagtg ccagaagaag 211260
gaacaggtgg acggggacca caggatcata gccgccggca ccgcggccgg ccgcaggaag 211320
ccgcccggcg cgtcgtctgt gtgcgggagc cgaaacaccg tgcctcttta tatcgtcccg 211380
acgtgacgcg agtattacgt gtcaggggaa aaccccgtca tgacgaacgt gattcgtaag 211440
tgacgcgggg tgctgacggg gttcggctcg agaagtgacg gagcgcctca cgtcagtatg 211500
atgtccgatc cgcgtcagcc ccgacgtggt tatggtcacc gaaacccacg tttatatgga 211560
cgttgagaac agcgcctgac cacatgattc atcataccat ttctcggaat cgggcccatg 211620
ccgggaaagc acattccttt tcagtaaaca acaatgacat cataacaaat cattttattc 211680
gcgaggtgga taataaccgc atatcaggag gagggatcgg gtgatgacgc aggccccgca 211740
aaacagtccg aaataaattt ttagtatcgc cccgtagtcg cctagatacc agaggtacgt 211800
caagttcatc aaaacgccca tcggcgtccc ggaatcgtat accgggcaca cgaagcgttc 211860
ataacaatcc cgggaggcga gtgttagggt agcagaatag tttcggggtc ggtttccttc 211920
cggcgacgac agctccgtgg gcagcagaat gtagagcgcc tcggtagccg tcgcggtgcc 211980
ttccacgagg atgggctgcc ggtgcctttc gtgattttct ccgtcgtgta gccaagccga 212040
ggcccgcaaa gtcttaggcg aggggaattg tccatagact ttcaccgcac ccttcagtac 212100
atggttctga ataacacagc cgcacgtgaa gtaggtcggt tctctcgtct cctccgtggc 212160
tgccgccacc actcccagcc accacaacag gcaggtcgcg agagggttcc ggaggcttcc 212220
ccggcgtagc atggtttcgg gttaaagcaa aaagtctggt gagtcgtttc cgagcgactc 212280
gagatgcact ccgcttcagt ctatatatca ccactggtcc gaaaacatcc agggaaaatg 212340
tcggtgcagc caacctttca catacagccc ccaaaacact tgaatcactg ccaccatcat 212400
cagcgtatac tgcgccgact taatcgtgag cgcgtagtac gccattagac ggcgatcttc 212460
gaacaatagt cgttcgatgt cctctaacga gctccacaga ggaacccaag gcacgaggca 212520
ccggggttcg cactctacat aataagtttg gcattggtgg cagggggaaa agtagaacaa 212580
cacgagtttt gtgcgttggg gaacacgata gtcccggagc cagtagcgtt ttgcgacgag 212640
gctttcggag acgtcctcca ccggcgtcgg cactcgatcc gcgtagccct ccagcgtctg 212700
gtagtacacc cggggtgtcg gcgtgggcac ggacaggttc ccgcgcaggg tccacagagc 212760
ctccagtcga ccgcccgatc ggagcacgca gcgcgcctcg gaatactcta ctcggtactc 212820
cgaaacatcg ggcagaggcg gtaacggctc cgtctccacc aagggcggag gttcatcgaa 212880
aagagtcaag gataattcag gcatactacc tgcgaccggg gcccagaggg ctaggataag 212940
cattacaaga ttcattctgt cttacaaggg aaggctgttc ccctgtctag actcaaaagc 213000
tgtaaggctg tcttatagca tgtagtcttg cacgtcacgg ggaacagggt ggtgatctag 213060
tgacgtcggg agaacacggt gttttagggt gcgggggaca aaggacagta cgacagatta 213120
ggtgatagaa acgttttttt ttatttatga aaaagccagt gtgccgtgcg gcctagggcc 213180
ccggcgtagt ttggatacca gatgggggcc gtcaggggta ctaccacgag cagaaacata 213240
ataacttggt ccatgtatag cagcatagcg gtgcgtagca ggtcgccgtc cgtgtagcaa 213300
tttgacggtg agcgataaag caccgttaat gtgtcgcgga taagcacgat cttgaggccg 213360
tagatgaagc tcacagtcag tgctaaaatg atgcgttggt atggttccca ggactgcacg 213420
gcgatgaaga gccagagtat gggaagcatg aagcttagca aacagaggat ggctaaccgt 213480
cgttgcatgt tccaggccat gagccaggct aggcccgtac accagacgca gagcatggat 213540
gacaggacat aggcctggat taccacggtg cgatcgaaac acagcccgat ggtggacacg 213600
gatatcgtag tgagggtggt atataccatg accagcatca gggtcccggg taggcgccga 213660
cgttccagcc agtacgcgtg gcaacgcaga gcgcagggta gcagtgtgct ccagaagggc 213720
agtgtatcgc gcaggtaggg ggtcgtcacg cgccacggta tgagcatgaa aaggatggta 213780
gtggctatgg tggcgctggt ctggaacacg acggtgccgt agagacgtac catccagaga 213840
aagtgttgaa cgctccgcag ggtgtcttca tctttggtga ttacggtgac tcgacggatc 213900
ggcggtggtg acggcggcga cacgggtggg ggtttctctt tcttatggcc gagtggctcg 213960
ccttggtgaa actggatctg taccatgacg ggtgctcgac gaacagtcgt cggggcttta 214020
ggtacccggc aagttttata gagaaagggg gacgatgggt ggtggctacg agccaccgcc 214080
accttcgcaa tacgaggatc tgaaggcggc aaagacggtc gtccagggca ggtgccagag 214140
gttgggactg agcacgatca gcgtgatttt aaacatggtc accagtccta cgtagatcag 214200
cagcgagcca cgtaacgtct gagcagccgg cagttcgtcg cggatgtaac gcgtgccgta 214260
gaaagtcacg gtcatcataa ggaagacgat ggcgccgtag ccgtagagta gaatacgctg 214320
atgatggaac acggtctggt cgccgataac ccagagcgtg atgaaaaaaa cgctggtgag 214380
cacccgtgtg catatgagct cccaacgctt agcgcgaaag ctgtccccaa ccatgacagc 214440
gccggtgcaa gctatccaca gcgtgaggac cagtgtgtag tcgatgagga tggcgggcag 214500
gtcggagcac caggtgtaga aaaccgtggt aacggagagg aggcctacgt agcccatggt 214560
caataccacg tcgtcggggt gcctttcgcc ctgtatcaag accaaacacc agagaaggga 214620
gggggcaaaa accagcagca gaggggaaga ttcatgttga catatgttgt gggaatcggg 214680
gatgcccagc caaatcattc cgcagaaagc cgtactgatg gcgatgtgaa agaccactag 214740
ggcgtagacc cggacgagga cagcaaaacg gcgcagccac ataaggccgt ggtgcagctg 214800
caggagagaa gcccattgcg gcgaatgtag cgacggcagc ggcgggtcca tgaggcgggt 214860
gatgcacccg agtgaacggg tgagcgtctc ggtggagtct tcttataaac cagcggagct 214920
caggcagtct tgctctggag cgtcgcagtg gtggtgttga ggatgacgct gagcgtgccg 214980
ttgtcaatcc ggtaatgatg ataggtgcca agcttggcca ggtagctgaa catttggtcc 215040
cagcgtgccg accacaccac gggcgtgagc atcaggagtg tggtgtgata aatgagtgtt 215100
tcggtggcgt aaagtatcag cgagctgcgg atgatgtggc tcacgggcat tttggtggcg 215160
atgtagcgca cgtcttggaa aagaacggcc aggatgcagc ccacgaacac ggtatagaga 215220
cacagcagag tcttatgcaa ccaggtgtaa gtagaagcca ggacgctgac catcaccgtc 215280
aaaagtgtgg aggtaaaaag cgcgtcacgc cacacggagc tgagacggtg ctcccaagcc 215340
acgccgttgc aggccacgaa caacgtccac gttaagatga ggctggaaac gccaatgggc 215400
gctgtggcgc acaggttgag cccggcggtg gtgaacgaca gaagcgccac atacagcgca 215460
aacaccaggc cgttgctggg gtgtctatga tcggtaagct ccagcgcgcc cagaaccaac 215520
accggtgtgc agctaagcaa taacggcgaa ggatcgtcgc ggcactcgta gcccagcgag 215580
gggtaaccca gccaaaccag cgcgctaatg agcacgctaa aagcggtttc cagcgtcagc 215640
aatccgtaga cacgcatgac gatcgcggtc cgccgtagcc aacacaccgc atcttcggaa 215700
gctgtggacg ctgtttccga ataccgggag gagatcgtgc ttccctcttc caaggatcgg 215760
aaagtagcgt ccgtcgtttc cgcagacgcg gcttccctgg tacgctccgt ttccgacgac 215820
gcggtttccc gctgcgtgga aactgtctcc atgtcgggac cgcagcgccc ggcggcgtat 215880
ccgcaaggtc tcgaagctac agcttgtcag aggaaaagta ggtttgcaaa aaggtgcgca 215940
gggtcatgat tctcagcacc atcagcagag tgaaaaccag gctgagaaac accttgacgg 216000
ccgccaaaag cgcgcgttcc agcggcgtct cgtagcgtac agccagggcc gcttcgtgga 216060
aatgcgagac ggctagacag gtaatgagca cgctgaagga caagacgatc ttaaagcacc 216120
aggaccaacc acgcctcaag atgaccacca cgattgccgt gaaggtcaac gtgatcaaag 216180
catggatgac cacgatctga cggcggacgg tacgttcggg agccaacaac gctacgccgg 216240
tgcagctgag aaaggccagt aaggtgaaca acgcggccga gatgaccaac gtaccgtcca 216300
ggcagagaca tatcacgatc aacggcggca cgtgaagcag cgtgtaaaag agcagaacgc 216360
cgatattgct gggatgcgat gtttcgtaac agtgaatgaa gatcaccgac gtgacgggta 216420
tgataaagac gaggctgggc gaggactccg tgagacacag acgggaatgg tgaaaccacg 216480
tcgcgggcgc cgcgtagcag aaggcgctca acaacgcggt caagccggcc agctgccaac 216540
ccacggcgcc ataggtgtgc agcgccacgc ggcaacagtc gacccaagcc agactgcggg 216600
tcgccagccg ggtctcttgg atcccggggg gcacgtagat gaccgtgcca tcggtgggta 216660
cctgaaaccc tttttctctt ctcatggtgc gctgcgttct ctggaaacgg ctgctctgtc 216720
cgaaaaccag ttccgaacga aaatctaggg cgagagggtg gacaacggcg tcgacgacga 216780
agcatgggac aggtcgttcg gcgttaacgt catcgcgtcg gacgacggta gttctaagag 216840
acgtagatcg ctcagcaggt cctgacagtt gcggattcgc aagatcagaa aaaaaaggga 216900
aatgaacgta ataaagagct gtagcgacgt atgcgctaca tcgcgtggca taagaacgtg 216960
acggacgaaa aggacctgct gcgaaaagtg gccggcaaag ataaggccca ccgtgctgta 217020
gaagcccaaa agcagccgca ggggccaagt ccagggccgc gtaaagacga tgagaacgtt 217080
aaccagaaag accacgaccc agacgccgtt gatgagggta aattgatcgg acagggtgca 217140
gttgtcgcga cagatgaaga ctacttccgc gcagagcaag gtgatgacca atgtgagcac 217200
aaacgacgtc aacacctcgc ggggctcctg gcaggcacac gtgacaccta gcgccgggat 217260
gtgcgccagg aggccggcga gtaatagcac cagctgtcgg aacggacgac ggcagcgcgg 217320
gtgccggttt cgctgagcga gaaccggtcg ctcataacgg aaatacacga agagcgcgga 217380
ggccacaggc accaggagga gcacctcggg cgcccagacg acgtgacaag gaaagcctgg 217440
acgcgactta agagtcgctg tagggaagac cagagagaag ctacccaaga cggccaccgc 217500
cgcggagatt tggaagagga gcaagccggc gattcggacg acaacctcga agcgatgcac 217560
ccagcccagc acggccacca cggccgcttc atcatagtcg tcgttgttgc cgctgtcgaa 217620
cagccgccga aacacgatct gtcgctgggt cgcggtggga aagcgcagac ccatgacagc 217680
cggaggctat atgaccgcgc gtctaaggcg cgagatccgt ggggggactt ttagatgttt 217740
gggcggcccg cggttctaac aggcttgatt ggtggagacg gccggcgcgg cgggtggggg 217800
aaacgacgag tttttccgtt acgccatggt tcgcgtgagg tttctctgta cctcccgcaa 217860
aaggtcacag cccgaaatgg aggccgcgtt ggtggccccg gtggcgcgtg acgataacca 217920
ggtcatccaa gtgatgagtt tgtctaatga gtcctcggtg gtgaagagga taagaatgag 217980
caggtacaag tacaccaggt tctcatagag acacaaggtg agcaggtcgg cctcggacca 218040
cgcgatctca aacaggcgtg tggtgtcaaa gaccgtaacg accagcatga agctgagcgc 218100
catggcgtaa tagcccaaaa aaagtttgtg ccccaacggt acgggctgca ggtaaagtgc 218160
gatcaagaac gcgataacgc cgatcacaaa cagcgtgacg atgacctgcc atcgacggcg 218220
attatggccg gctagacccg tgacgcagct gcagaggcta aaaagcacgc aagccaagag 218280
gcccgagaag gtcaccagcg tagaggagga gcaggcgctg gccacgatca ccgaaagcgt 218340
cgtgagcacg ctataaatgg tgagcaggcc cgggctcggc ggcgacgtaa acgatccttc 218400
atcgcgtttg ccatgcagca gggccaaaca gatggtgggc accatcaaac tcaagggcgg 218460
cataaagccg gtgcaacaga gaaagacggt gcctttaaga tgcggaaaag ccagcaccag 218520
gcccagacag agcaagaagg tgcaggtgcc ctgcacggcc acggtgctgt agacccgcat 218580
acaaagtaaa aagcgacgta cgtcgttcgt cgagacggag gaaatcataa tgactccgcg 218640
cgagggtcgc gggggtgggg gcgcccaggc cgtcccggtg gcctctgagt tcggagacat 218700
gacggcggtg gctatcaaaa ggcgcgtatg agaaaccgtt tatagagtgt aatagaatca 218760
ccgtcattcc cacacggcgt tcccccataa agtcacgtca cactcgagta agcgtgaaaa 218820
agctttatta ttgaataaaa aacacgagta caacaccgag ttgcggtgtc ctgtctactg 218880
ggtgggggag gtttatcgtc tgtctctaga gggaaggtgg ggaacgtcta agcgagcggg 218940
agcgtgtcat ctcccccatc tttttacaac aagctgagga gactcacgcc gtcgatgcgt 219000
ccgccgtgtt tctcggcgta ctgctgcacc cagacgtggc cgctaaagat ggcgacgctc 219060
atgtttagga gactcatgac gatggtgtac aacacgacgc tgacacagac gctgttttta 219120
gacagcgttc cacgctggta gatgagatcc agggtctcgt aaataagcac ggccgaagcg 219180
gcggtcacca ccaggacgta gagtccgctg tagatcttgc tgacccacag cacgggcgaa 219240
aagtaaagca ataggtaaaa gacgatgacg gaccagccgt agccaatccc gatgactttc 219300
cagcgcgtgg gattgttgcc ggccaggtag gtgagaccgc tgcagagaac gaaaaagacc 219360
atcaccaggg caaacgacag accgatgacg cgcctttctc cgcaaaagcc cgtgcacacg 219420
gtgatgccgg tgttgatcag caggcatgcc accgtgagat gagcaaaatt ggtggtgtgt 219480
gggcgaaact cggcgaaacc gcgtagcatg gccagcgtgg acacgggcac gatggaggac 219540
agggctggca ctatgccgtt ggcgcactgt ccctgcacat cggggaaggc gagccaagcc 219600
agcaggcaga ccgtgagggt acaagccagc tgccacacga gcccgtgata gacctccatg 219660
agcagcttga agcgtttcaa ccactggaag agctgctgtt cggccaccag cgcgtggctg 219720
cgatggagcg gcacgatggt gaccgtcggc gactcatggt gttcggaaac cgaggcggtg 219780
tcgcccatgc tgccgcttac gaccgctgtc ggtctaaggt aggcgtcgat gaaacagtcc 219840
gtcttatcag cacccggtta ccgcggattt gattgacgtc acgagtgtgg tcaaaccgtg 219900
gcggcaccct gtatccgacc cgtcgtcatg ggctccacaa ccagagcctc agaagatggt 219960
acatgccgat gaataaagcc acattttcga catagaggcg tagcgagggc tgaaaactct 220020
ccgggaaaga actctgacag gtgatcaggg acagatcgtg aattagcatc agcgtcaccg 220080
tcaacagcgt cgtcgcgtgt aaaccgagaa agaacggggt cgcggcccgc agcagccaaa 220140
gtcccagcgc cgtagcgcag agcagagaca ggaccgacgg tagccacagc cgccggagag 220200
acgcgccagg atcgcaaccc aaaagcgagg cccccaggca gccgagatct accgccaggg 220260
cgagaagagc cgcgccgaga aaggcctgcg gcgacggctg gcacatcagc aaggtcagaa 220320
aggctagcgc gtgcggcagg cagtaagcca acaggagtgg gagtttgcgg ggacaacggt 220380
cgatagacgg accgcgtagc agcaggaaca ggcagccgac gggcacgacg aggctgagat 220440
gagaaagcgg cggtgggtcg tcgtcccgtc cccgctcgca tagctcggcc accggtggcg 220500
gcatgagcca ccagctgagc acgctgaggg cgacggtggc ggtaagctgg aaggcgacga 220560
ggacggaggc gcgcagccat accgccagcc tctctaagta ggggactacc tcctcgacgg 220620
tccattctag cgggacgaca tgaagcatgg cgacaagcgc ggctgctgtg aaaatgagcg 220680
cggttttata ggcattagga cttcccgatc gtactggcgg ctgtcaaagt cccgttgtcc 220740
aaagacgcgc cgtccgaaag actaatccaa cggggacccg agagcatgag caacaacgtg 220800
agaaagatgg ccatgctgtc caggtagaga cagacggcat gacggatgca ctggttaggt 220860
gggcagaaaa agatgaccat gagactgtcg taggccagaa tacccaaaaa gaagctgatg 220920
gagaaggcgc acaacgtcac cactatcttc tgcagccagt cggcgtcgct tagcagagcg 220980
agcgtgagga acgaaagcag catcaccacg tagacgcagc tgatgcattt ccaacgacgt 221040
cggtcacggc cacctagaaa cgccagcccc gtaaaggaga taaacaacgc cagggtcatc 221100
acgtaggaac ctactagtac gcggctttca gagcacattt ggaagatggc cgccgtcagg 221160
ctgttggcca acagatagat gaaaagcacc gtggcgttac tagggtgctc gttgcccaaa 221220
gtgtacgtga tgaacatgca gacgatgggc acgagcacgg tgagaaagaa gctgtagttc 221280
tcgacgcaaa agttgcggtt ttgtgggaac cccaaccaaa aaacgcttcc caagccgaag 221340
ctgaaagcca actgaaagat gaagatggcg tacacacgca gccatacggt gaactttttg 221400
aaccactcga gagcctccat gcgggagagc agcagcgcgt tagcctcctg cgcctgcatg 221460
gtggcgacgg tctcggcaca aagccgctgc ggcgcaccta cccttctctt atacacaagc 221520
gagcgagtgg ggcacggtga cgtggtcacg ccgcggacac gtcgattagg agacgaactg 221580
gggcgacgcc gctgctgtgg cagcgaccgt cgtagcgacc gtcgtctgag cagtgtgggc 221640
gctgccgggc tcggagggca tgaagtagag cacggagaca aagaggtaca tgaggtccat 221700
gtacaagcag agcgcgcccg ggatataact ctcatactcg atgtcgtgca ggatgtcctg 221760
cgtatcgcac accaccgagg tcacgatgac ggccaaaccg gctatcatca ccaggatctc 221820
acttaccgcc tcgggaaaaa gagaaaatac ggcgaacagt aagagaatca gcgtggatgc 221880
gcccgtcaat agggaacgct gtaattccac gtcgcgggca aacagatacg tagcgagcgt 221940
aaggaaacaa aatagcgtta ctgtggccac catggcataa atgactgaac gatgactaaa 222000
atggaagcct gacgccgtga cagccacgct ggtaagcaac gtgtacgtca gtaagatcca 222060
tacgtttttg ggaaagttgg gctcggccca acgcaacaga cctaggcaca cgatggagat 222120
cattaagcaa gacagcgtca gacgcacgct ggaaaagagc tgctccagcc ggtgcggcaa 222180
caccagccag caaaaggcgc agacgctcat aaggatgagg cattgcaccc agataaggat 222240
gtagatgcgc agcaggaaga ccgaccgggc tatctggacc tgaccgcgga gcgacatggc 222300
ggcaacgccg gcggttatcg ccgagattcg tctaaataca cgaagcgaac tagaaaacgc 222360
acacacgtta tttgcaaaaa gaaagcagct gccggcttat tattttatta aaaatttatc 222420
tgtgcagaat cataagttta tgatgaataa aaacggggaa agggaatctg cttttaggga 222480
cccgggtctg gtccgtcgtc tcccatctgg tcgggttcgg ggatggggac ctgtttcagc 222540
gtgtgtccgc gggcgtgcat ggcttttgct cgccggccgc gctgtaacca ggcctctttc 222600
tctgtggtcg gcgagtcttc cgacgggtag ggagtctggg agtccatcgc ttcaggccca 222660
ccgctcgttc cctcgaccgt cgtgtcgtcc tcgttttcgc tattacacgg ggtttctgga 222720
gtatcgccta tacggttggc gattctccgg gggtggccgc tctcgtcctc gtcgctgcta 222780
tcgccgcccg gtaattcgac gccgcattcg ttgtacggag cgcggcacat gggcggcgga 222840
aagaacttgg gcatgcgaaa gcagcgttgt ccatccacgg tctgcgtggt ttcatcatta 222900
tcctcccata atcccccctg tagcgccggc agcgtttcga cgctgtgaga ggggaaggcc 222960
cagttctggt tgtcttgcag cgcgcccgtg ggcagtaggt ccgtgcggcc ccaggcgctg 223020
ctgttgttgg gtaccttgtc agtgccgcga gtaggtcgca gaaaccagtc cagagcgctc 223080
tctagctgcg agcgtgtgat ggtgcccagt gcgccgtgcc agcgcagcac gtctcttttc 223140
agcgtgtggt gacagacggg cagctcctcc aaccgacact cgccgcgcaa tccgcggtcg 223200
aagcggcaga gaccacgcaa tttaagcaga ccgcacttga gaaacatgtg aaaattatcg 223260
gcaatgcgat acaggtctga gtcctcgatc ttgtgtaggt agaccacgcc aaacttgtcg 223320
agcagcacca ggccgctggg cacaaaaggc ccgtaggcca ggtaatagcc cacgaggccg 223380
acgacgtacc actcgcagca caagcgttga cgaataaagt tcagaagatc gcgaaagtcc 223440
gcggccggca tgtggtcaaa aggccggcag gcgcgcaggc cctcgatgga gcccagcatg 223500
agcaacggct ccacctcggt gcgacccggc gtgcggatga ccaggttgag accgctcatt 223560
tcgcgggccg tcttggccac ggccgcagcg tcagtggggt cggtgcagag gaatttttgc 223620
acatgatagc gcggttcggt ggtggcgaac ggcgtttgtg ggtgccgata cacatattcg 223680
caccagagta ggccgttctt ggaaaaggct ttgatatcac tggccacctc gtagagcccg 223740
tcggtctccc agtcgtagac gtagacggtg ccgtaatgac ttagcatgag cacgcagggc 223800
agttcctgcg cctgcttggt gtttcgtgtt agatcgctgt cgggtggacg cacggctagt 223860
acaccgacgg cttccagggt gtcatcgcag cagagatagt cggcggccag agaacgtgcg 223920
taaatctgcg ggatggcggc ctgttcgcgc atcactagga accagttggc ggggttgcgc 223980
agtgctacgg tggttccttg gtggcgctgc acgtaggttc tcagcgccgg aggatcgtac 224040
tggcgcagat agaggccttg cagcatcgat aacgtctttt gaaagacggt gtttctaaat 224100
tggaaaacgc cgtagtcgca gcggatagca tcttcgcagc gctcgtcgcg ctgtcggaga 224160
taggtgcccc aggcttcggc ggcggctttg gtgagtaggg acatgccggc ggagccgtct 224220
cgacagcgag tcggataaag cgcgctgcgc gaaagcttaa tataggagca gcgtcagacg 224280
aatcgcggct ggtggcccgg ggggtgggac gcgccgccta cacaaaatgc tcccgaaaat 224340
cgaaactctt gacccactcc ggagacaaat ccgtattcag attgatgcgt cgcgcttcca 224400
cttcggcttc cgaaacctcg gcctccgtcc ggtaggcgtt aacaatacgc tgacccaggt 224460
gccaacgctc tttctctgcc aaacgccgtt gctcaaacca ctcgtctacg tccttgaggt 224520
caaagacagt gtcctcctca aggtcaaagc ctaggtcttc ccactcgtcg tcatcgctct 224580
cgtggccggc ggccatacgc gcggcaaccg cgtcttcccc tcctcttctt tcaacgttgg 224640
gtaccacgtt gttttcttcg ggttccatgg gttctgcgcc actatcgtca tcgtcctctc 224700
cctgctcctc atcgtccgcc aaggcgtcgt ggatcacctc caggttctga ttgtcgggta 224760
cgacgtggtt atcttcgtcg tcgtcgcgtg gcatgggcgg cggccgacgg cggacgaccg 224820
gcatggcgcg gccgtcgttt ccttcgtctt cctcttcacc gtctcccaag gaacgcggtc 224880
gacgacgttc cgcgaagtcg ccgcggacca cgcgcgcctg ccaaatggta aacgcgtccc 224940
aaccgtccca gttattgagc atttcggcgc gaaaacggtc gcctcgacag agccagcgaa 225000
actgccgcgc gtagtcgcgg tctacgccgc tgtcgaacat ggtaaagtgc agacgcgccg 225060
cctcgcccat gtgtacgcag cctccattgc gttccagcct ggccgcgcgc cgcagaccgt 225120
gttcgtagcg gcgacgcacg tacaccttca tgaggccggc gcgaaaaagt tcctctaggc 225180
tgtcggccag acggtagatt tcaccggcta gacgctgcag gggcggcgag cggtccagat 225240
gcgacttgac aatcaccacg taaaaacgac agaaacggtc gaagatgatg aggaaggacg 225300
tgtcaaagaa accaccggcg cggtaggagc ccacggcgcc tagcaggtac cagcggcaac 225360
gcagttgcag cgtgacgtac atttcgcact cggccaagcg ggcggctggc gctacctcga 225420
agggccagca atccgtcaag cagccgaaac tggtcaggag tttcaacgtt ttggcatggc 225480
gcccaggtgt gtgaaagttc acgtcgcgtc cgtggtgttc gccaacgcag gcggccaacg 225540
cgtcggcgtc atgagcgtga cgcagcagca tcgctaccac gtcgtgcggt acccgcgtag 225600
caaacggcgt ctgtggctga cggtatacgg cttcggtgta catcataccg taacgtgcca 225660
gctcgtccag atgacgcgcg cacagcagca gaatctcttg cgagggttcg tagatgtaga 225720
ggcgcgtacc gccccccatg cagagcacca gctccgtctc ttcgtagtga tcttccacca 225780
tgatcacgca cttgcctagc acgataaggc gttcggggca acaaatcacg tcgtccagca 225840
gttggtcgcg cagctccggc atggtgctgc caggccgcac ctgcaggaac cagttgtgcg 225900
gaatgccgag cgacaacacc tggtcgacgt ggttacggac ccagtcgcga agcacgtcgg 225960
cgctgtactg gcactcaaag atgccctgaa agtcgctcat gacccgcaga aaagtttcgt 226020
agcgcgtgtg gcaatagagg aattcatcgt ttcgcgtgaa cgtgggagct ccgtcttccc 226080
aacgtgtacg ccacatgtca aaagaggccg ccagctagac accccagaaa agaagcagag 226140
aaagagagtt ctttgtgcga cacgttttat tccgcgtcct ccgctcgacg ctcaaatctg 226200
gatgtactcg cgcacacccg tcaggctctt taagggaaaa gggtccgagt acgtcactaa 226260
ccgcgactga tgcaccaggg cggtaatcac ccgctctgcg ccctcgcgcg tcgacgaacg 226320
cgtcgtcacc aggcagtgca gccgcgggcc cgtatcgtcc tgatgaccag cggcctcgcg 226380
ctcggctgct tccacaccga caatgtcggg atccaacacg tagctctgcg agttggtgtc 226440
gtagcggtgt aacaccaacg tgttggggtc cagacgctcc cacgcgccct cgtgcgggtc 226500
aaaacgctcc gttaaacaga gccagtcata ctgctgctgc agaatacgcc gctcgcgctc 226560
gcgtcgctca tcgggcaacg cagcgtcttc gttgaagaga atgtcccgct tgtggtctac 226620
ggcacgctcg tggtggtgcg ggcacagatg acggtgttcc atacgcgtct gacgctgacg 226680
ctcgcgttcg aagcgccggt gtcgaaagac cattttcagc aaccccatgc ggaaaaactc 226740
cgtgatggtg ttggcaacgc gccgcacata gtggttgggg tcgtccatct ggatggcgta 226800
cacggcaccg aaccagtcca gcagtaccag cacttcggcc acaaagttgc gtcccggtcg 226860
cggacgtccc gtcacgccta gcacatacca cggcgtggcc agattagcac ggacagccca 226920
ccaccaacga cggctctcca cctcggtgag cgcacagaag ggccaaatgc ggtgtaactg 226980
ctgcaccgtt ttcatcagcc gcataatcac cgtaccgtaa cccggtgtat gcaacttcac 227040
gtcgcaaccc aggattcgtt cggccgtggc gtacgagccc tcgggcgtgg tgtcattgag 227100
aaacaaaaca tgcatggtac gcgcgccctt agggtatcgt cgcggaacag gtaccgtcat 227160
tctccgcaga gtggtgtgaa tcacgtcgcg atacgcaatc tccgaacgcg acacaccgta 227220
acgtgccagt tcatccaagt tgtgcgatac taacaccatg tacttttcac gagtgtcgta 227280
ggcgtagacg cgagaaaagc gacccataaa aaccacgtac ggggtagcca ccatgccatc 227340
atggtgatcg cgacgtggct cgggcaacaa aataacagcg tatcccaacg gcgtcagcgg 227400
ctcgcggcaa cagatgagct ttgacgccgc ctgtctggcg gcggtaatga tcccgtcctc 227460
cgtacgtaac atcacatgcc agcccttggg gggacccaag gacagacaac gtccctcgtt 227520
acgatgaacg taacgcgtga tttccattgg ctccaggcaa aagaacagtt ccttaaaatc 227580
ccgcaacact tgtcggtata acgccatggg atcctcggcc gccacaggca gcgcggggag 227640
ctccggcggc acaactgcag cgccgtcagg gccagaaccc gcagccggat ccatcattac 227700
gcgacactct cagccggaca accggcgtca ctgacagaag ccgagccaaa tacagagaaa 227760
gcaacgctac accgtcaccc cgctcccaag cgccgcggaa agtgctccga tttttcaccg 227820
tcgttcgcga cgttgatttg cctcggtctg agaaccgacc tagcgttcgg accggtgcgc 227880
agaaacagcc ggcggtccga gccactgagc ggttcacagc cccggccgcc gatagttacc 227940
ggagagacgt tcgagctgca ggtacatcgg cgctccccgc ttcgccaccc cgcgcccgcc 228000
ccagtttata ctctccgacg ccccgtccaa cgcgcctgtg gagggccaat cggaccgcgg 228060
gagctctcca agtggatgac aggcacagcc gggtgcccga ccgtgaagag ccctcatcca 228120
cctgaacaga ccgctaaccg aaggaccccg agtcgcgtcc gtcggtcccg acgtccgtcg 228180
ccatctggct ccctgctgtt ggctacctct cggatttcaa aaaagagcac gtgccgatga 228240
cggtgcacag gaaagagcca aagtgtcacg gcgtcttttt ttatttgtat tcctttcctg 228300
ttttgtactc gtaaactgtt gacgttgttt ttacatccaa aagggcaagt aagaaacagg 228360
atgaggcatg gtaggtttgg gcgtggggcg gccctccagc acggcggccc gggccgcccg 228420
gcgggtgagc acccggcgtt gcgccgtatc tatcttgtgt ttcttctgtg tctttttcct 228480
atcttgttcc gcgacggcct ctttcatcac gttcagcatg cgttcctcga cgccctccag 228540
ggatcctggg gaggagggag tcctagtgag gcttccaatg ttgttttgtg gattttcggt 228600
ttcctcttct tggtcgtcat cgtcggacgt gtcgtcttcc tcttgatcct cttcttcgtc 228660
cgagtagtag acgcatagtc cctggttcat caggctggga ttcatcaggt tctgacgggg 228720
aatccgctgt tgtagacgtt taaccgcccg ttccaggcga gagctcatgc cgcaccagac 228780
gctgtaacgc cgcacgggcc cgtagcgggc tgtttgttcg cgtacatgat cgttgagctc 228840
ttgccaatat tgtttggcac actccagatc ggaggtttgt ggatagtcgg gtcggatccg 228900
cggatcccaa ctgacatcgg cggtgccaga gacttcgtcc agactgttac gcatagagca 228960
ccagtcgggt cggacgataa acctgtcctt gcggattaac catttataac gtagttcgtg 229020
atggcgtgta gaggcccgta cacgctccac ggtcccaaag cggtcccaga agggaaagtt 229080
ttcgtggggg cagcgacccg gcacttccaa acgttcggcg tcgtccacgg cgtagtggaa 229140
acgccggccg gcctggtaaa ttttgagcag acccacggtt aacaacatat ccacgctgtc 229200
agccaaccgc cagatctcgc gccgagatac gtcaaaatag aaaaattcgc aggctcggtc 229260
gaccaggatc acgaaatcgg cgtgaaaaac gccggagggt agcgactcgc ccaccacacc 229320
cattatcatg gtttcacagc ataagcggtc cacaaagaac ttcaacaggt cgttgaattg 229380
ctccgtctcc atacagatga agggccagac gcctttgagg ttctcggcct ggccgcagag 229440
cagcaacgga cgcgtcatct cgcctggagt gcgcagaggc acgcattcgc cgcgataacg 229500
acaggtcaca cgctgcagtt cgctgatgct gttgtcgtgc aggcgaaggt cgcagataat 229560
atgatccggt tgcgtggtta gcagcggcgt gcgcatttgc tcgccgtaga tggcctcgca 229620
gtgcaatagc ccgtgtcgtg caaaatcgtc cagactgtgc gccaggtagt aaagcacccc 229680
gcgatcgcgg tctagacacc acacggtttc gtaacgtcct agcagaagca ccagacgggc 229740
ctggctaggt ggctcaattt cctctacata cacgaaaaag tcgtcatcgt ccgagtcctc 229800
gtcctcagaa gaggaccgcg gcccgtgtac tctgggcaac acggtggtag agaactgcag 229860
gacgcccaga gactcgagcg actcttcgca gcagatgagc tgaccccagg gcgtttctgg 229920
cccgtcggtg acagccgcgc tgccaaagat gtcctcaaac tctacaaaat ctagacgcca 229980
tccgggtggc gctgaaacgg gaaggctaat gttcatatca gcatagctac gaactaagtg 230040
gcggatgtcc tgccgcaagt cttggcagag aatgagcttt cgtaaaccct tgagggtcct 230100
ccgaacaacg gccccagacg cgtagcgata ggactggcgc atggtgccgc ggcgtggagc 230160
ggcacttggc agcctatttt atggagtttc ttcagtgacg tggcttgttc acgtcgttcg 230220
tgggctgcgg ttggcagctc cggtctgtaa accacccgaa aagactgaca tcgacgtcaa 230280
agacccacgt aatttggaac atgtgcgacc gcaaagtgcg tcagaataac acgtggcttt 230340
aggacataaa aagtaccgtg aggtccagac gtggtttttg tgattgacac ttacaccagg 230400
taagccaagg gacggtgaaa ctgtatgtga ggaacctggg tgcttagacg actaacgtgt 230460
aatgcttttt acaggactgt tcgacaggtg atagtacctg taaggtgatg accacctcta 230520
caaataatca aaccttaaca caggtgagca acatgacaaa ccacacctta aacagcaccg 230580
aaatttatca gttgttcgag tacactcggc tcggagtatg gttgatgtgc atcgtgggca 230640
cgtttctgaa cgtgctggtg attaccacca tcctgtacta ccgtcgtaag aaaaaatctc 230700
cgagcgatac ttacatctgc aacctggctg tagccgatct gttgattgtc gtcggcctgc 230760
cgttttttct agaatatgcc aagcatcacc ccaaactcag ccgagaggtg gtttgttcgg 230820
gactcaatgc ttgtttctac atctgtcttt ttgccggcgt ttgttttctc atcaacctgt 230880
cgatggatcg ctactgcgtc atcgtctggg gtgtagaatt gaaccgcgtc cgaaataaca 230940
agcgggctac ctgttgggtg gtgatttttt ggatactagc cgtgcttatg gggatgccac 231000
attacctgat gtacagccat accaacaacg agtgtgttgg tgaattcgct aacgagactt 231060
cgggttggtt ccccgtgttt ttgaatacca aagttaacat ttgcggctac ctggcgccca 231120
ttgcgctgat ggcgtacacg tacaaccgta tggtgcggtt tatcattaac tacgttggta 231180
aatggcacat gcagacgctc cacgttcttt tggttgtggt tgtgtctttt gccagttttt 231240
ggtttccttt caacctggcg ctatttttag aatccatccg tcttctggcg ggagtgtaca 231300
atgacacact tcaaaacgtt attatcttct gtctatacgt cggtcagttt ttggcctacg 231360
ttcgcgcttg tctgaatcct gggatctaca tcctagtagg cactcaaatg aggaaggaca 231420
tgtggacaac cctaagggta ttcgcctgtt gctgcgtgaa gcaggagata ccttaccagg 231480
acattgatat tgagctacaa aaggacatac aaagaagggc caaaaacacc aaacgtaccc 231540
attatgacag aaaacatgca cctatggagt ccggggagga ggaatttctg ttgtaattcg 231600
atcctctctc acgcgtccgc cgcacatcta tttttgctaa ttgcacgttt cttcgtggtc 231660
acgtcggctc gaagaggttg gtgtgaaaac gtcatctcgc cgacgtggtg aaccgctcat 231720
atagaccaaa ccggacgctg cctcagtctc tcggtgcgtg gaccagacgg cgtccatgca 231780
ccgagggcag aactggtgct accatgacgc cgacgacgac gaccgcggaa ctcacgacgg 231840
agtttgacta cgatgaagcc gcgactcctt gtgttttcac cgacgtgctt aatcagtcaa 231900
agccggtcac gttgtttctg tacggcgttg tctttatctt cggttccatc ggcaactttt 231960
tggtgatctt caccatcacc tggcgacgtc ggattcaatg ctccggcgat gtttacttta 232020
tcaacctcgc ggccgccgat ttgcttttcg tttgtacact acctctgtgg atgcaatacc 232080
tcctagatca caactcccta gccagcgtgc cgtgtacgtt actcactgcc tgtttctacg 232140
tggctatgtt tgccagtttg tgttttatta cggagattgc actcgatcgc tactacgcta 232200
ttgtttacat gagatatcgg cctgtaaaac aggcctgcct tttcagtatt ttttggtgga 232260
tctttgccgt gatcatcgcc attccacact ttatggtggt gaccaaaaaa aacaatcaat 232320
gtatgaccga ctacgactac ttagaggtca gttacccgat catcctcaac gtagaactca 232380
tgctcggtgc tttcgtgatc ccgctcagtg tcatcagcta ctgctactac cgcatttcca 232440
gaatcgttgc ggtgtctcag tcacgccaca aaggtcgcat tgtacgggta cttatagcgg 232500
tcgtgcttgt ctttatcatc ttttggctgc cgtaccacct gacgctgttt gtggacacgt 232560
tgaaactgct caaatggatc tccagcagct gcgagttcga aaaatcactc aagcgcgcgc 232620
tcatcttgac cgagtcactc gccttttgtc actgttgtct caatccgctg ctgtacgtct 232680
tcgtgggcac caagtttcgg caagaactgc actgtctgct ggccgagttt cgccagcgac 232740
tcttttcccg cgatgtatcc tggtaccaca gcatgagctt ttcgcgtcgg agctcgccga 232800
gccgaagaga gacgtcttcc gacacgctgt ccgacgaggt gtgtcgcgtc tcacaaatta 232860
taccgtaata aaaaagcgct acctcggcct tttcatacaa accccgtgtc cgcccctttt 232920
ttccccgtgc ccgatataca cgatattaaa cccacgacca tttccgttcg attagcgaac 232980
cggaaaagtt tatggggaaa aagacgtagg aaaggatcat gtagaaaaaa catgcggtgt 233040
ttccgatggt ggctctacag tgggtggtgg tggctcacgt ttggatgtgc tcggaccgtg 233100
acggtgggtt tcgtcgcgcc cacggtccgg gcacaatcaa ccgtggtccg ctctgagccg 233160
gctccgccgt cggaaacccg acgagacaac aatgacacgt cttacttcag cggcacctct 233220
ttccattctt ccgtgtcccc tgccacctca gtggaccgtc aatttcgacg gaccacgtac 233280
gaccgttggg acggtcgacg ttggctgcgc acccgctacg ggaacgccag cgcctgcgtg 233340
acgggcaccc aatggagcac caactttttt ttctctcagt gtgagcacta ccctagtttc 233400
gtgaaactca acggggtgca gcgctggaca cctgttcgga gacctatggg cgaggttgcc 233460
tactacgggg gttgttgtat ggtgggcggg ggtaatcgtg cgtatgtgat actcgtgagc 233520
ggttacggga ccgccagcta cggcaacgct ttacgcgtgg attttgggcg cggcaactgc 233580
acggcgccga aacgcaccta ccctcggcgc ctggaactgc acgatggccg cacagaccct 233640
agccgttgcg atccctacca agtgtatttc tacggtctgc agtgtcctga gcaactggtt 233700
atcaccgccc acggcggcgt gggtatgcgc cgctgtccta ccggctctcg tcccaccccg 233760
tcccggcccc accggcatga cttggagaac gagctacatg gtctgtgtgt ggatcttctg 233820
gtgtgcgtcc ttttattagc tctgctgctg ttggagctcg ttcccatgga agccgtgcgt 233880
cacccgctgc ttttctggcg acgcgtggcg ttatcgtcgt ccacttccaa ggtggaccgc 233940
gccgtcaagc tgtgtcttcg gcgcatgctg ggtctgccgc cgccaccgtc agtcgcacca 234000
cctggggaaa agaaggagct accggctcag gcggccttgt cgccgccact gaccacctgg 234060
tcactaccgc cgtttccgtc cacgcggata cctgacagtc cgccgccacc gtaccagctt 234120
cgtcacgcca cgtcactagt gacggtaccc acgttgctgt tatatacgtc atccgacatc 234180
ggtgacacag cttcagaaac aacgtgtgtg gcgcacgcta cttatgggga acccccggag 234240
cccgctcgat cgacggctac ggttcaggaa tgtaccgttc ttaccgctcc aaattgcggc 234300
atcgtcaaca acgacggcgc ggtctctgaa ggccaagacc atggagatgc ggttcaccat 234360
agcctggatg tggtttccca gtgtgctgct gatactgggg ttgttgacgc ctccgagtaa 234420
cggctgcact gttgatgtcg gacgaaacat gtccattcga gaacagtgcc gccttcgaaa 234480
cggtgcgacg ttctccaagg gagacatcga aggtaacttc agtgggcccg tcgtcgtgga 234540
gttggactac gaagacatcg atattactgg cgaacggcag cgacttcggt tccacctcag 234600
cggactcggg tgtcctacaa gggagaaaat aagaaaagat aatgaaagcg acgtcaacgg 234660
tggaattcgc tgggctctat atatacaaac cggcgacgcc aagtacggta ttcgtaatca 234720
gcatttgagt atacggttaa tgtatcctgg ggaaaaaaat acacaacagc tgttgggttc 234780
tgatttcagt tgcgaacgtc accggagacc gtccacgccg ttgggaaaga acgccgaagt 234840
gcctcccgcg acccgcacgt cttctacata cagcgtcctc agcgcttttg tagtgtggat 234900
cggatccggc ctcaatatca tctggtggac cggcatcgtg cttctggcgg tggacgctct 234960
cggacttggc gagcgttggc tgaggttagc actgtcccac cgggacaaac atcacgcatc 235020
gcgaaccgcg gcgctccagt gtcaacgcga catgttactt cggcaacgtc gacgggctcg 235080
gcggctgcat gccgtttctg aaggcaaact gcaggaagag aagaaacgac agtctgctct 235140
ggtctggaac gttgaggcgc gaccctttcc gtccacacat cagctgattg tgctgccccc 235200
tcctgtagcg tcagctcctc ctgcagttcc ctcgcagccc cccgagtatt cgtctgtgtt 235260
tccgcctgta taaaaataaa gagacgggag gctgatcgcg gccttcagcg tctcatttgt 235320
ctttactctc gagtgcggtc ggtgtctcgt cggtgagacg aggccgccgc ccgacaagtt 235380
cgatctcatg tcgctcttgg agcgcgaaga gagttggcgt cgcgtagtcg actactcgca 235440
caacctgtgg tgtacgtgcg gtaactggca gagccacgtt gagattcagg acgaagagcc 235500
caactgcgag cagccggagc ccgcacactg gctggaatac gtggcggtcc agtggcaggc 235560
ccgggttcgc gattctcacg atcgctggtg tctctgcaac gcctggcgtg atcacgcttt 235620
gcgcggccgt tggggtacgg cgtattcctc gggttcctcg gcctcttcct ccggtttcgt 235680
cgcggagagc aagttcacct ggtggaaacg actgcgccac agtacccggc gctggttgtt 235740
tcgccgccgg cgagctcgat acactccatc taactgtggg gaaagtagca ctagcagcgg 235800
ccagagtagc ggtgacgaga gtaactgcag tctacgcacc cacggcgtgt acacacgggg 235860
tgaacaacac taatcgataa gtcgcgtgta ggcgactggc tacatcaacc ggatatctgc 235920
ggggatttaa aaagacgacc cgttgtcatc cggcttagag caaaccgtcc ttttatcatc 235980
ttccgtcgcc atggctatgt acacatccga atccgaacgc gactggcgtc gtgtaatcca 236040
cgactcgcac ggcctgtggt gcgactgcgg cgactggcga gagcacctct attgtgtgta 236100
cgacagccat tttcagcgac gacccacgac ccgagccgaa cggagggccg ccaattggcg 236160
gcgacagatg cggcggttac accgtctgtg gtgtttttgt caggactgga agtgtcacgc 236220
gttatacgcc gagtgggacg gcaaagaatc cgacgacgag tcgtcggcgt cttcctcggg 236280
cgaagcgcca gagcaacagg tccccgcttg gaagaccgtg cgggccttct cgcgggccta 236340
ccaccaccgc attaaccggg gtctgcgggg cacgccccca ccgcgcaact tgccgggata 236400
cgagcacgcc tccgagggct ggcggttttg cagtcgacgg gaacggcgag aggacgatct 236460
tcgcacgcgg gctgagccgg accgcgtggt gttccagtta gggggagtac ctcctcgtcg 236520
tcaccgagaa acttacgtgt aagaacacgg cgtgacaata aacaacatag cgtaaatccc 236580
cgtgtgatgt gtgtgattga cgttcgggaa acatgtcccc atcatcagcg tcacaactga 236640
cgtgggttgg tcactgacgt gcaggatgtt gcgcgagtca gagaatcgca taagaacggg 236700
gtggtgagcg ggttcccaca ggagtctctg gcgcaaaagc accatgagcc tcaggttccc 236760
cgagagggcg ggttacgaga aactgggata ccgcccgcat gccaaacgcg tgcgggtgca 236820
tgacccgttg ggattgacgc ggtttatcat gaggcaactc atgatgtacc cgctggtgtt 236880
gccgttcacc tttccgtttt acgtgccgcg gtcctagcac gtcagtggtg atgctgataa 236940
ttgcaacatg gcccatgacg aacccgcttg ggacgaacgt caataccacg tcaaaccacc 237000
gtgacttggc tgaacgttga aacataaagc caaagcgccg tcggcacttg gcttcagagc 237060
agcgcctcgg ggcgatgcga cggcgatgaa cttagagcaa ctcatcaacg tccttggtct 237120
gctcgtctgg attgccgctc gtgctgtcag ccgcgttggt ccgcatggct ccggactcgt 237180
ttatcgtgag cttcatgatt tctacgggta tctgcagctg gaccttctgg gaccagtggt 237240
ggcggggaat cgctcagtcc ggacctggaa agagcaggcg gaccgagcca gagggacctt 237300
cgttcggcgt tcaggcctta atactagcta catcttacct gtcggcggcc tgtctggggg 237360
ctccggtacc ttacccgtcg gcctgtatcg tcccgaagaa gaggtgttcc tcctcttgaa 237420
ccgctgccat gggccactgt caacgccgaa aaatgcttgt ctggctgagg tcggtgtcgc 237480
taatgccact tttttgtctc gcttcaatgt cggtgatttt cacggagcgt catgggaaaa 237540
cggtaccgct cccgatggag agcccggggt atgctgaaat tcctcttaag attccgtaaa 237600
cgacgttgtc cagtcgttgt gccgcgattc gtacggttca tcgtctacgt cgttttgttc 237660
accgtcgctg tgcaacgcgt gaaacaagag cgtgatgcgc accttcggcg gtatgaagaa 237720
cggttacgga aaaaccgcgc acggcgtcgg cagtcttttc cgtgacttgg ggcgatgggt 237780
ccgagctgcg gtatgggtca cggcggcgtg tgttttattg acgaagatgc cgatgtgtga 237840
ctaaaaacgt cccagcccta gagcgatgtg tttcaataaa aattatgtcg tatcatagta 237900
tgcgtgtcct ggtttttcat ttttggatgt atttgtgaca taaaaggcga tagaatgtgg 237960
ggacgaaaca tatccagata cacagttttg ttattcgaac aaaacccgtg tgatgcagaa 238020
aacagtactg caggatgaaa gtcccatggg gggggggggg cagacagtag tcgtttttgc 238080
cgctgggcgt acgctatgct tgtatttatg actataatat gtgcactcgt gtgtcgatgt 238140
tcctattggg aagggtgtca atgtaggagg tataaagaat ggtgggatgc ggagaggcat 238200
cgctagacac aggttgatcg ctgtgctagc cccacctgat cagcgtcatg ggtaaagcgg 238260
tgattaagcg tgaaaacacc gtaagggggg gggggcagac aggaagcttg gtggcagtgg 238320
ccgttagatg cattacgtgt ctgtattggt acatttgcaa accgtcgggt gtggcggtat 238380
agtttagcga tgattatatt atgtatgtgc cgtatagaat ggcctaaaac attgtaacac 238440
gaaacgttac aatgatggga aagatgccga taaaaaacac ataaaaggca tatacacgaa 238500
ttactagtta cacgtttgtc tatgtgcgag tttaaggacg cttgtataat gcgtatgacg 238560
gcaaacggcc gcggaaacga tggggggggg gggtagtaac tgtattaatt atacgtcttg 238620
cagtacacgg tattgtgtgc tggtgcgcgt attacgacac gaacggcata gcgctataac 238680
cgggtgtatg gtatttatat gtgcgtctag catccttgcg agattctgaa agtcttcttg 238740
taagcgtaat taaaacggtg tatgttctgc gtaaagtgca ttcaaacaac gtaacagtat 238800
gggatgaatg ccaataaata acatataaaa gcgagaagta tacatataag ggttgctaga 238860
cacaggtttg tttctgtgct agcccaatgg cacttgtaca atccatgcaa gcaaaaaaag 238920
gatgcgaaac caacatcgtg gggtgggggg ggggtaaaag caatgttaat cattggtctc 238980
gcggtgcaag ttgctgcgtt ttacgtgtat tgttacacgg gttgcgtatc ggtataatcg 239040
gatgtgtgtt actcattcgt ggcgttgtta tagtattgtg aaaaagaatt ctcgtaagca 239100
tgttgacaac tgcaaaataa aaccatttta ttgagcattg taatggtagt gtgtcgctac 239160
attagaaaac gtgacgcgtc gcatgtcgcg gcacaatctg gcagcggggt cggggtaggg 239220
tacggtggga ggcatgtaca cagatggaac aaaagcagaa gtaacgtgag acggagcata 239280
tagtccagta tccagcggtt cctgagtagc accacccatc aactgaatgc cctcatgagt 239340
aaaagtctgc gggcggcagc ccttggggac cgttggcatg ggacgatcga tctccaaacc 239400
acagcgtaac acggttttct tccaacgtcg ttgatacacg tcgtttttac ggttactccc 239460
cagaacccag aaagtctcgt ccaagtcgta ccaggagtct tccccaggga gacgtggcgg 239520
tttccaatcc tcatcgtccc gtcgcaaagc acgtcccaaa ctggcttggg gagtcaacgg 239580
tggttctgtg ggtcgggtgt agcgcgagtg ttttccgttc atgagcgatt cgtcctcctt 239640
gcctttaggc tttttggcct ttttgtgtat catctggccg ccggcctcca taaccaccgt 239700
ggccaagtcc agtcccagag cttgagcgtc ggcgcggcgt cgggcgtctt gcaggtagtc 239760
ttccacattt gcacagatgg ccgggtgttt ggtggctagg gtgaggacct cagcctcgcc 239820
gcggcccgga cgtagcaaaa aagctaactg cccgtgcggc tcgcgcgccc acagcgcggc 239880
gcgcgggtgc aggtgcagcg cgtcccagcg cggccgctcc cactgctcgc ggtccagctc 239940
gggcagcagc cgccgcgcgg cctcggcggc gggcgccgac tcgcgcccca gcgccagcgc 240000
gcccaacacg cccgcgcgca gaaagtgcga cagctccgcc gccagcgggt acacgtgccc 240060
gtccagcggg cagtacccga acacggcgcc cagctcgtcc agcaccacca ccagcatggc 240120
gcgcggcacg gtccccgacg ccgccggacc cgccatcgcc gtcggaccca ccatcaccgt 240180
cggcgccgcc gctgctgccg ctgccgcatc cgttccgacc accgcgtgcg cgtccgcgtt 240240
tggcacgcaa atcgcgctcc cgccggcggc gccgtacggc tgcggaggta aagtcacagc 240300
agaccccacg gctcccgcca tcgcgcacgg cgcgtccccg ccggcggcct ccgtctccgt 240360
gccgctcgcc cccggcagca acgtcgtccc cgtcgccatc gccgtcgtcc ccgccgtcat 240420
cgtcgtcgtc gtccccgccg tcgtccctgt cgccggccct gccgcgcagc gcagccaccg 240480
cgacggcagc accgcgccca gcgccagcca gccgcagcac agacgctggt tcaggtgccg 240540
acgcacggcc gtcagcagcg acgcggggtg cggcgccgac gcgaacggct cgtactgcgc 240600
cagctcctgc cacgcgccca gcagcaccat cggctgcagt cgcctgcccg gcgtctgcag 240660
cgccaccgtc gtgccggccc accgccggcg cagctcccgt ccgagcgccg tcgcctcctc 240720
ggcgcgcagc aacgtctggc gaagcgccgg ctgaggcagc agcgtcgcgc gcggggtgcc 240780
cacgcccagc cggttgcagc ggtacaggcg caccacctcg cccgcgccgt gccgaaacca 240840
ctcgtccgcg tcgcgcgccg ccaggatcag cgtgttgttc gccaggtcgt acacgaacac 240900
gcggaacccg gcgcccagcg ccaggtacag tccgtcctgc gcgcacagac cctcgggatg 240960
gccggccttg tcgcccaccg tcgggtcggc cgcggggtcc acctcgtgca ccacggtcgc 241020
caccagcacg atccacgcgt cccgcggcga cagctgacgc aggtccgtgg cgcccacgcc 241080
gttcatctgg ctgcgcggcg tcactcgcgc gtagaatccg tacggccgtc cgagcggcag 241140
cagcgtgccc gcgtcgcgct gcgaccactt gcgcatggcg cggcccgtgc tgttggccaa 241200
aaacgcggcg cgccacacgg cgcccatggc ctggtattcc agctccgtca gcgcctggcg 241260
ctccaccgga atctgagaca gcagcaagcg ctccgggccg tgccaaaagg tgctgttgtt 241320
gccgctaccc ggaggggcgc ccggcggccc gcggggttct acccggtgga cggcgtgggc 241380
cggcgtcgcc gtacccgcag tactcgtact agtccccgct gttgacgtcg cttccaaaga 241440
agaagaacga gaggaaccaa cccccgaagg ccctccggct ccgcggccgc gaccgagggg 241500
cggggggcgc ggcgacatgc cgttgcgctg ggccatggcc gccggacgcc tccgacgtcc 241560
actctgtata tataggaagc aaacccgcgt cagcgaccac gccgtttaca cacgcggacg 241620
cctccgtcgc ccgtgtgccg cgggcgacac gcacctggct tttataggca gcgacgtgca 241680
cggcgcttgc tggcgccgcc ttgccgccgc gcagtctgga aggccgtgaa aaaaccgaaa 241740
gaagatgcgc gacaagccac caacaacggg ccgccgagcg cgcgcacacc taggtggacg 241800
cctgacctcc attccgggcc gtgtgctggg tccccgaggg gcgggggggt gttttcatcg 241860
ggggggtgaa atgtgggaat ttgggaagtt ggcggtggcg ggggacggcg acggcgaata 241920
aaagcgccgt gcggcgcgca cggcgaaacc cagacgcgcg tgtgtcttgc gtctctttca 241980
gtcgcgcgtc tgtcttgcgt gtctttgagt ccccggggaa aagaggaagc ggtcccgagg 242040
ggacggcggc gcgactccct ggggacgcga agatcggacg cggaaaagag gaagtccccg 242100
gggacggcca caggcggaaa aaaaaaacga ggaagccgca gcccagtctg ccgggaccgg 242160
caggcaggcg cacagacccg cgtcgaggac acacgcagaa gaagcgcccg cgccggggcg 242220
ggggggggga tcgcgggccc cggggcacac tgcttccatc tggccgcgcg cacaccccgc 242280
cgacacaccc ctgacacacc cgcgacacac ccggcacacg cccgcgacac acccggcacg 242340
cgcccgccac acagccagcc gacacacccg cgacacaccc gccacaccca gccgcacccg 242400
gcacacaccc acccagccgc acccccgcca cacccagacc gacgccggtg cgggaccggg 242460
ctccattccg ggccgtgtgc tgggtccccg aggggcgggg gggtgttttc atcggggggg 242520
tgaaatgtgg gaatttggga agttggcggt ggcgggggac ggcgacggcg aataaaagcg 242580
ccgtgcggcg cgcacggcga aacccagacg cgcgtgtgtc ttgcgtctct ttcagtcgcg 242640
cgtctgtctt gcgtgtcttt gagtccccgg ggaaaagagg aagcggtccc gaggggacgg 242700
cggcgcgact ccctggggac gcgaagatcg gacgcggaaa agaggaagtc cccggggacg 242760
gccacaggcg gaaaaaaaaa acgaggaagc cgcagcccag tctgccggga ccggcaggca 242820
ggcgcacaga cccgcgtcga ggacacacgc agaagaagcg cccgcgccgg ggcggggggg 242880
gggatcgcgg gccccggggc acactgcttc catctggccg cgcgcacacc ccgccgacac 242940
acccctgaca cacccgcgac acacccggca cacgcccgcg acacacccgg cacgcgcccg 243000
ccacacagcc agccgacaca cccgcgacac acccgccaca cccagccgca cccggcacac 243060
acccacccag ccgcaccccc gccacaccca gaccgacgcc ggtgcgggac cgggctccat 243120
tccgggccgt gtgctgggtc cccgaggggc gggggggtgt tttcatcggg ggggtgaaat 243180
gtgggaattt gggaagttgg cggtggcggg ggacggcgac ggcgaataaa agcgccgtgc 243240
ggcgcgcacg gcgaaaccca gacgcgcgtg tgtcttgcgt ctctttcagt cgcgcgtctg 243300
tcttgcgtgt ctttgagtcc ccggggaaaa gaggaagcgg tcccgagggg acggcggcgc 243360
gactccctgg ggacgcgaag atcggacgcg gaaaagagga agtccccggg gacggccaca 243420
ggcggaaaaa aaaaacgagg aagccgcagc ccagtctgcc gggaccggca ggcaggcgca 243480
cagacccgcg tcgaggacac acgcagaaga agcgcccgcg ccggggcggg gggggggatc 243540
gcgggccccg gggcacactg cttccatctg gccgcgcgca caccccgccg acacacccct 243600
gacacacccg cgacacaccc ggcacacgcc cgcgacacac ccggcacgcg cccgccacac 243660
agccagccga cacacccgcg acacacccgc cacacccagc cgcacccggc acacacccac 243720
ccagccgcac ccccgccaca cccagaccga cgccggtgcg ggaccgggct gaggggggtg 243780
taagcgcctt gacgcgcggc gctatggcac tgcgtgccac ggtattggtt ggcgggacga 243840
ggctgagggg ggtttgcggg acgtaagcgc gacgccgagt ttgcggcgtg ctgtggcgcg 243900
ctcaaggcta gggacggggg ccgcgcgtag cttttcggcg gcgacgggga acaaaacagg 243960
cacgcacacg cagctcgctt cagcggtcgc cctatcggca ccgccgctat tctttattaa 244020
cgtcgttctc cccccgcttc tacacgcgga ccgctgcaga cggctatgct cagactgttt 244080
ctcttgacgg cgacatgtgt ttgcgtctgt gcggcgcggc gacggccgca agcggacgac 244140
gccgcctgcc accacacgtg gcccacgcgc tgcgccggcc cgcgtcccgc gccgtcggct 244200
cacaacacgg cgctttcttc ctcgcacgcc atctccatcg cacaccgtgt gctgcattcg 244260
ccgtcgcctc cacgagagaa catccgccac agcatgcgct gtcgccgtcg cgccatggcc 244320
tcctcggctt gcacacccgt ttcgcacacc cagcctcggg ccgccaacca cagccgttcg 244380
aggatgacgt acgcgacgag cgagcccacc aattctccca cggcctcgcc cgccaagtca 244440
gac 244443
<210> 2
<211> 21
<212> RNA
<213> Artificial Sequence
<220>
<223> siRNA
<400> 2
gcuacaagcu ggagaaugau u 21
<210> 3
<211> 21
<212> RNA
<213> Artificial Sequence
<220>
<223> siRNA
<400> 3
ucauucucca gcuuguagcu u 21
Claims (51)
- (1) 적어도 하나의 이종유래 항원을 코딩하는 핵산;
(2) UL78 유전자의 불활성화 돌연변이; 및
(3) 활성인 US2, US3, US6, US7, UL97 및 UL131A 유전자
를 포함하는, 재조합 간시클로비르-민감성 TR 스트레인 인간 시토메갈로바이러스 (HCMV) 벡터. - 제1항에 있어서, 활성인 US2, US3, US6 및 US7 유전자가 HCMV의 AD169 스트레인으로부터 유래하는 것인 재조합 간시클로비르-민감성 TR 스트레인 HCMV 벡터.
- 제1항에 있어서, 적어도 하나의 이종유래 항원을 코딩하는 핵산의 발현이 UL82 프로모터, UL7 프로모터, UL45 프로모터, UL78 프로모터 또는 US13 프로모터에 의해 추진되는 것인 재조합 간시클로비르-민감성 TR 스트레인 HCMV 벡터.
- 제1항에 있어서, UL78 유전자의 불활성화 돌연변이가 UL78 유전자 전부 또는 일부의 결실인 재조합 간시클로비르-민감성 TR 스트레인 HCMV 벡터.
- 제4항에 있어서, 적어도 하나의 이종유래 항원을 코딩하는 핵산이 UL78 유전자의 전부 또는 일부를 대체하는 것인 재조합 간시클로비르-민감성 TR 스트레인 HCMV 벡터.
- 제5항에 있어서, UL78 유전자의 전부 또는 일부를 대체하는 적어도 하나의 이종유래 항원을 코딩하는 핵산의 발현이 UL78 프로모터에 의해 추진되는 것인 재조합 간시클로비르-민감성 TR 스트레인 HCMV 벡터.
- 제1항에 있어서, UL7, UL38, UL45, UL82 및 US13으로 이루어진 군에서 선택되는 HCMV 유전자의 불활성화 돌연변이를 추가적으로 포함하는 재조합 간시클로비르-민감성 TR 스트레인 HCMV 벡터.
- 제7항에 있어서, UL7 유전자의 불활성화 돌연변이가 UL7 유전자 전부 또는 일부의 결실인 재조합 간시클로비르-민감성 TR 스트레인 HCMV 벡터.
- 제8항에 있어서, 적어도 하나의 이종유래 항원을 코딩하는 핵산이 UL7 유전자의 전부 또는 일부를 대체하는 것인 재조합 간시클로비르-민감성 TR 스트레인 HCMV 벡터.
- 제9항에 있어서, UL7 유전자의 전부 또는 일부를 대체하는 적어도 하나의 이종유래 항원을 코딩하는 핵산의 발현이 UL7 프로모터에 의해 추진되는 것인 재조합 간시클로비르-민감성 TR 스트레인 HCMV 벡터.
- 제7항에 있어서, UL38 유전자의 불활성화 돌연변이가 UL38 유전자 전부 또는 일부의 결실인 재조합 간시클로비르-민감성 TR 스트레인 HCMV 벡터.
- 제11항에 있어서, 적어도 하나의 이종유래 항원을 코딩하는 핵산이 UL38 유전자의 전부 또는 일부를 대체하는 것인 재조합 간시클로비르-민감성 TR 스트레인 HCMV 벡터.
- 제12항에 있어서, UL38 유전자의 전부 또는 일부를 대체하는 적어도 하나의 이종유래 항원을 코딩하는 핵산의 발현이 UL38 프로모터에 의해 추진되는 것인 재조합 간시클로비르-민감성 TR 스트레인 HCMV 벡터.
- 제7항에 있어서, UL45 유전자의 불활성화 돌연변이가 UL45 유전자 전부 또는 일부의 결실인 재조합 간시클로비르-민감성 TR 스트레인 HCMV 벡터.
- 제14항에 있어서, 적어도 하나의 이종유래 항원을 코딩하는 핵산이 UL45 유전자의 전부 또는 일부를 대체하는 것인 재조합 간시클로비르-민감성 TR 스트레인 HCMV 벡터.
- 제15항에 있어서, UL45 유전자의 전부 또는 일부를 대체하는 적어도 하나의 이종유래 항원을 코딩하는 핵산의 발현이 UL45 프로모터에 의해 추진되는 것인 재조합 간시클로비르-민감성 TR 스트레인 HCMV 벡터.
- 제7항에 있어서, US13 유전자의 불활성화 돌연변이가 US13 유전자 전부 또는 일부의 결실인 재조합 간시클로비르-민감성 TR 스트레인 HCMV 벡터.
- 제17항에 있어서, 적어도 하나의 이종유래 항원을 코딩하는 핵산이 US13 유전자의 전부 또는 일부를 대체하는 것인 재조합 간시클로비르-민감성 TR 스트레인 HCMV 벡터.
- 제18항에 있어서, US13 유전자의 전부 또는 일부를 대체하는 적어도 하나의 이종유래 항원을 코딩하는 핵산의 발현이 US13 프로모터에 의해 추진되는 것인 재조합 간시클로비르-민감성 TR 스트레인 HCMV 벡터.
- 제1항에 있어서, UL128 유전자 또는 UL130 유전자의 불활성화 돌연변이를 추가적으로 포함하는 재조합 간시클로비르-민감성 TR 스트레인 HCMV 벡터.
- 제20항에 있어서, UL128 유전자 및 UL130 유전자의 불활성화 돌연변이를 포함하는 재조합 간시클로비르-민감성 TR 스트레인 HCMV 벡터.
- 제1항에 있어서, 적어도 하나의 이종유래 항원이 병원체 특이적 항원 또는 종양 항원인 재조합 간시클로비르-민감성 TR 스트레인 HCMV 벡터.
- 제1항에 있어서, 적어도 하나의 이종유래 항원을 코딩하는 핵산의 발현이 내인성 프로모터에 의해 추진되고, 적어도 하나의 이종유래 항원을 코딩하는 핵산이 섬유모세포를 통한 다중 계대시 안정한 것인 재조합 간시클로비르-민감성 TR 스트레인 HCMV 벡터.
- 제1항에 있어서, 제2 이종유래 항원을 코딩하는 핵산을 추가적으로 포함하는 재조합 간시클로비르-민감성 TR 스트레인 HCMV 벡터.
- 제24항에 있어서, 제1 이종유래 항원을 코딩하는 핵산이 UL78 유전자의 전부 또는 일부를 대체하며, 제2 이종유래 항원을 코딩하는 핵산이 UL7, UL45 및 US13으로 이루어진 군에서 선택되는 HCMV 유전자의 전부 또는 일부를 대체하는 것인 재조합 간시클로비르-민감성 TR 스트레인 HCMV 벡터.
- 제25항에 있어서, 제1 이종유래 항원을 코딩하는 핵산의 발현이 UL78 프로모터에 의해 추진되며, 제2 이종유래 항원을 코딩하는 핵산의 발현이 UL7 프로모터, UL45 프로모터 또는 US13 프로모터에 의해 추진되는 것인 재조합 간시클로비르-민감성 TR 스트레인 HCMV 벡터.
- 제24항에 있어서, 제1 이종유래 항원이 병원체 특이적 항원 또는 종양 항원인 재조합 간시클로비르-민감성 TR 스트레인 HCMV 벡터.
- 제27항에 있어서, 제2 이종유래 항원이 제1 이종유래 항원과 상이한 병원체 특이적 항원 또는 종양 항원인 재조합 간시클로비르-민감성 TR 스트레인 HCMV 벡터.
- 제24항에 있어서, 제1 및 제2 이종유래 항원을 코딩하는 핵산의 발현이 내인성 프로모터에 의해 추진되고, 제1 및 제2 이종유래 항원을 코딩하는 핵산이 섬유모세포를 통한 다중 계대시 안정한 것인 재조합 간시클로비르-민감성 TR 스트레인 HCMV 벡터.
- 제1항에 따른 재조합 간시클로비르-민감성 TR 스트레인 HCMV 벡터 및 제약상 허용되는 담체를 포함하는 면역원성 조성물.
- 제30항에 있어서, 대상체에서 면역 반응을 유도하기 위해 투여되는 면역원성 조성물.
- 제31항에 있어서, 재조합 간시클로비르-민감성 TR 스트레인 HCMV 벡터의 투여가 적어도 하나의 이종유래 항원에 대한 장기 이펙터 기억 T 세포 반응을 유도하여 유지하는 것인 면역원성 조성물.
- 제24항에 따른 재조합 간시클로비르-민감성 TR 스트레인 HCMV 벡터 및 제약상 허용되는 담체를 포함하는 면역원성 조성물.
- 제33항에 있어서, 대상체에서 면역 반응을 유도하기 위해 투여되는 면역원성 조성물.
- 제34항에 있어서, 재조합 간시클로비르-민감성 TR 스트레인 HCMV 벡터의 투여가 제1 및 제2 이종유래 항원에 대한 장기 이펙터 기억 T 세포 반응을 유도하여 유지하는 것인 면역원성 조성물.
- 제1항에 따른 재조합 간시클로비르-민감성 TR 스트레인 HCMV 벡터를 코딩하는 단리된 폴리뉴클레오티드.
- 제36항에 있어서, 서열식별번호: 2 또는 서열식별번호: 3에 나타낸 서열을 추가적으로 포함하는 폴리뉴클레오티드.
- 제36항에 따른 폴리뉴클레오티드를 포함하는 단리된 세포.
- 제38항에 있어서, DAXX 유전자의 발현을 침묵화하는 siRNA를 추가적으로 포함하는 단리된 세포.
- 제38항에 있어서, 포유동물 세포인 단리된 세포.
- 제40항에 있어서, 인간 세포인 단리된 세포.
- 제38항에 있어서, 섬유모세포인 단리된 세포.
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Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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KR1020217001036A KR102403547B1 (ko) | 2014-07-16 | 2015-07-16 | 외인성 항원을 포함하는 인간 시토메갈로바이러스 |
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US201462025348P | 2014-07-16 | 2014-07-16 | |
US62/025,348 | 2014-07-16 | ||
PCT/US2015/040807 WO2016011293A1 (en) | 2014-07-16 | 2015-07-16 | Human cytomegalovirus comprising exogenous antigens |
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CN107002048B (zh) * | 2014-07-16 | 2021-07-06 | 俄勒冈健康与科学大学 | 包含外源抗原的人巨细胞病毒 |
MA40783A (fr) | 2014-10-03 | 2017-08-08 | Los Alamos Nat Security Llc | Vaccins contre le vih comprenant un ou plusieurs antigènes episensus de population |
EP3256595A4 (en) | 2015-02-10 | 2018-09-26 | Oregon Health & Science University | Methods and compositions useful in generating non canonical cd8+ t cell responses |
MA43285A (fr) | 2015-11-20 | 2018-09-26 | Univ Oregon Health & Science | Vecteurs cmv comprenant des éléments de reconnaissance des microarn |
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MX2019004518A (es) | 2016-10-18 | 2019-06-17 | Univ Oregon Health & Science | Vectores de citomegalovirus que provocan celulas t restringidas por moleculas del complejo mayor de histocompatibilidad e. |
US11617788B2 (en) | 2018-07-09 | 2023-04-04 | Arizona Board Of Regents On Behalf Of The University Of Arizona | Viral promoters and compositions and methods of use thereof |
GB201906104D0 (en) * | 2019-05-01 | 2019-06-12 | Univ Plymouth | Intrinsic systems for viral vector transgene regulation |
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