KR102188074B1 - Composition for alleviating skin regeneration and atopic dermatitis - Google Patents

Abstract

The present invention relates to a composition for skin regeneration and alleviation of atopic dermatitis, which effectively regenerates skin tissues that have been lost due to wounds or died due to aging, and has an effect on alleviating itching related to atopic dermatitis and anti-inflammatory. , Apricot Seed Oil (Prunis Armeniaca (Apricot) Kernel Oil) 20.0-52.0wt%, Jojoba Seed Oil (Simmondsia Chinensis (Jojoba) Seed Oil) 25.0-35.0wt%, Evening Primrose Oil (Oenothera Biennis (Evening Primrose) Oil) 20.0- 30.0wt%, Juniperus Communis Fruit Oil 0.5-3.0wt%, Lavender Flower Oil (Lavandula Spica (Lavender) Flower Oil) 0.5-3.0wt%, Orange Peel Oil (Citrus Aurantium Dulcis (Orange) Peel Oil) 1.0-3.0wt%, Tea Tree Leaf Oil (Melaleuca Alternifolia (Tea Tree) Leaf Oil) 0.5-1.5wt%, Marjoram Flower Oil (Origanum Majorana Flower Oil) 0.15-1.0wt%, Scented Geranium Oil (Pelargonium Graveolens Oil) 0.15-1.0 wt%, Orange Flower Oil (Citrus Aurantium Dulcis (Orange) Flower Oil) 0.1-1.0 wt%, Chamomile Flower Oil (Anthemis Nobilis Flower Oil) 0.05-1.0 wt%, Jasminum Officinale Flower Oil It is composed of 0.05-0.5wt%.

Description

Composition for alleviating skin regeneration and atopic dermatitis}

The present invention relates to a composition for skin regeneration and atopic dermatitis, which effectively regenerates skin tissues that have been lost due to wounds or died due to aging, and has an effect on alleviating itching and anti-inflammatory related to atopic dermatitis.

Atopic dermatitis is a recurrent chronic dermatitis with severe itching, and about 10-15% of children have atopic dermatitis. Such atopic dermatitis generally improves in adulthood, and about 30-40% of dermatitis does not recur, but the rest is known to cause severe dermatitis such as dry skin, skin irritation by irritants, housewife eczema, etc. The situation is gradually increasing as environmental pollution intensifies.

Atopic dermatitis is often caused by overlapping with other diseases.In particular, when atopy does not emit prenatal fever and accumulates on the skin, causing rash and itchiness, frequent use of steroid ointments and antihistamines leads to addiction, resulting in extremely low immune function. It can be considered a skin disease.

Therefore, as a part of the treatment of atopic dermatitis, pharmaceutical compositions have been developed, but most of them contain steroids or antibiotics as active ingredients, which induce telangiectasia or increase/dilation of the thickness of the stratum corneum when used for a long time. There was a concern of causing serious side effects such as.

On the other hand, in relation to the cosmetic composition for atopic dermatitis, a composition containing ringworm, cloves, gardenia, sculptors, and tea plantains as active ingredients has been proposed, but most of these include heating the plant natural product itself at high temperature or using an organic solvent. Therefore, there is a problem in that various side effects occur due to the destruction of active ingredients and skin irritation or the addition of preservatives for long-term preservation.

Therefore, as a prior art for solving this problem, Korean Patent Publication No. 10-2017-0061805 discloses a'composition for treating atopic dermatitis'.

The above technology is composed of Sendella asialica extract, neomycin sulfate, which is a bactericidal antibiotic, and salicylic acid as active ingredients, and it is suggested to minimize the occurrence of side effects from use and to have excellent efficacy in removing atopic factors. It was done.

However, the above technology had the effect of overcoming diseases related to atopic dermatitis to some extent, but the skin tissue at the treatment site was lost, or above all, the treatment-related efficacy itself did not work for users suffering from specific allergic diseases. Became.

Korean Patent Application Publication No. 10-2017-0061805 (2017.06.07. Composition for treatment of atopic dermatitis) Korean Patent Publication No. 10-2015-0035276 (2015.04.06. Composition for preventing and treating atopic dermatitis containing rapamycin and mycolphenolic acid as active ingredients) Korean Patent Application Publication No. 10-2011-0130555 (2011.12.06. Atopic skin prevention and improvement cream manufacturing method)

The present invention is to solve the above problems, and the object of the present invention is to effectively regenerate skin tissues that are lost due to wounds or cell death due to aging, and at the same time have excellent effects in relieving itching and anti-inflammatory related to atopic dermatitis. It is to provide a composition for skin regeneration and atopic dermatitis relief.

The configuration of the problem solving means of the present invention for achieving the above object,
Apricot seed oil (Prunus Armeniaca (Apricot) Kernel Oil) 39.2wt%,
Jojoba seed oil (Simmondsia Chinensis (Jojoba) Seed Oil) 28.0wt%,
Evening Primrose Oil (Oenothera Biennis (Evening Primrose) Oil) 22.5wt%,
Juniperus Communis Fruit Oil 1.5wt%,
Lavandula Spica (Lavender) Flower Oil 2.2wt%,
Orange Peel Oil (Citrus Aurantium Dulcis (Orange) Peel Oil) 2.2wt%,
Tea Tree Leaf Oil (Melaleuca Alternifolia (Tra Tree) Leaf Oil) 1.1wt%,
1.0wt% of Origanum Majorana Flower Oil,
Scented Geranium Oil (Pelargonium Graveoles Oil) 0.9wt%,
Orange Flower Oil (Citrus Aurantium Dulcis (Orange) Flower Oil) 0.9wt%,
Anthemis Nobilis Flower Oil 0.3wt%,

The composition composed of 0.2wt% of Common Jasminum Officinale Flower Oil was heated to 40-50℃ in a reduced pressure stirrer, and then stirred under reduced pressure of 300-400mmHg, and then lecithin and organic acid monoglyceride were added to the composition. Ride, sorbitan fatty acid ester, polyoxyethylene fatty acid ester and sorbitan stearate any one or a combination of two or more selected from the group consisting of a dispersant further comprises.

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The composition for skin regeneration and atopic dermatitis relief according to the present invention effectively regenerates skin tissues that are lost due to wounds or cell death due to aging, and at the same time, complementarily expresses each substance that relieves atopic dermatitis. It is very effective in relieving itching related to sexual dermatitis and anti-inflammatory.

1 is a graph showing the experimental results on the skin regeneration effect of a composition for skin regeneration and atopic dermatitis relief according to a preferred embodiment of the present invention.
Figure 2 is a graph showing the experimental results on the skin dermal cell regeneration effect of the composition for skin regeneration and atopic dermatitis relief according to a preferred embodiment of the present invention.
3 is a graph showing the experimental results on the β-hexosaminidase secretion inhibitory ability of the composition for skin regeneration and atopic dermatitis relief according to a preferred embodiment of the present invention.
Figure 4 is a graph showing the experimental results on the Histamine secretion inhibitory ability of the composition for skin regeneration and atopic dermatitis relief according to a preferred embodiment of the present invention.
Figure 5 is a graph showing the experimental results on the antioxidant effect of the composition for skin regeneration and atopic dermatitis relief according to a preferred embodiment of the present invention.
Figure 6 is a table showing the results of analyzing volatile fragrance components of a composition for skin regeneration and atopic dermatitis relief according to a preferred embodiment of the present invention.

Hereinafter, a composition for regenerating skin and alleviating atopic dermatitis according to a preferred embodiment of the present invention will be described in detail.

The composition for skin regeneration and atopic dermatitis relief of the present invention includes apricot seed oil (Prunus Armeniaca (Apricot) Kernel Oil) 20.0-52.0 wt%, jojoba seed oil (Simmondsia Chinensis (Jojoba) Seed Oil) 25.0-35.0 wt%, Evening Primrose Oil (Oenothera Biennis (Evening Primrose) Oil) 20.0-30.0wt%, Juniperus Communis Fruit Oil 0.5-3.0wt%, Lavandula Spica (Lavender) Flower Oil) 0.5-3.0wt%, Orange Peel Oil (Citrus Aurantium Dulcis (Orange) Peel Oil) 1.0-3.0wt%, Tea Tree Leaf Oil (Melaleuca Alternifolia (Tra Tree) Leaf Oil) 0.5-1.5wt%, Marjoram Flower Oil (Origanum Majorana Flower Oil) 0.15- 1.0wt%, Cented Geranium Oil (Pelargonium Graveoles Oil) 0.15-1.0wt%, Orange Flower Oil (Citrus Aurantium Dulcis (Orange) Flower Oil) 0.1-1.0wt%, Chamomile Flower Oil (Anthemis Nobilis Flower Oil) 0.05-1.0 It is composed of wt%, including 0.05-0.5 wt% of Jasminum Officinale Flower Oil.

The apricot seed oil (Prunus Armeniaca (Apricot) Kernel Oil) contains 11 essential amino acids and various mineral elements, and is a natural substance known to have an effect of maintaining clean skin by removing aging dead skin cells. According to a preferred embodiment of the present invention, the apricot seed oil is included in an amount of 20.0 to 52.0 wt% based on the total weight %.

The jojoba seed oil (Simmondsia Chinensis (Jojoba) Seed Oil) is a liquid ester oil obtained from the seeds of jojoba, has excellent stability and texture, and is widely used as an oily raw material mainly in basic cosmetics. This jojoba seed oil is an oily fat with less oily feeling than other vegetable oils, and it is easy to be familiar with the skin because it has a structure of a wax ester, a component of sebum. For this reason, it is mainly used for skin care products such as emulsion, cream, body oil, and hand lotion, and makeup products such as lipstick and foundation. According to a preferred embodiment of the present invention, the jojoba seed oil is included in an amount of 25.0 to 35.0 wt% based on the total weight %.

The evening primrose oil (Oenothera Biennis (Evening Primrose) Oil) is a vegetable oil extracted by compressing the seeds of evening primroses, and is a vegetable oil extracted by compressing the seeds of evening primroses as a plant of the needle flower family with short hairs throughout the plant. It has excellent skin moisturizing and regenerating effects, and is effective in relieving premenstrual syndrome, menstrual pain, and menopausal symptoms. According to a preferred embodiment of the present invention, the evening primrose oil is included in an amount of 20.0 to 30.0 wt% based on the total weight %.

The Juniperus Communis Fruit Oil is known to be effective in stimulating metabolic activity and removing inflammation, and may be used for the purpose of treating oily skin, acne, and various skin diseases. According to a preferred embodiment of the present invention, the Dosong fruit oil is included in an amount of 0.5 to 3.0 wt% based on the total weight %.

The lavender flower oil (Lavandula Spica (Lavender) Flower Oil) is effective in relieving stress and sleeping well, relieves pain such as headache, muscle pain, menstrual pain, etc., and can be applied directly to the skin in a small amount, so dermatitis, mild burns, psoriasis, etc. It is useful for acne and insect bites. According to a preferred embodiment of the present invention, the lavender flower oil is included in an amount of 0.5 to 3.0 wt% based on the total weight %.

The orange peel oil (Citrus Aurantium Dulcis (Orange) Peel Oil) relieves tension, is effective in improving chronic anxiety and depression, and helps relieve stress. In addition, it has the effect of improving anxiety such as excitement and loneliness, which are symptoms of menopause and tension before menstruation in women. In particular, it has excellent regeneration effect of skin cells, and has good effects on dry, sensitive and aged skin. According to a preferred embodiment of the present invention, the orange peel oil is included in an amount of 0.1 to 1.0 wt% based on the total weight %.

The tea tree leaf oil (Melaleuca Alternifolia (Tra Tree) Leaf Oil) is a powerful immune stimulator that improves body immunity, promotes sweat secretion, lowers body heat, insect bites, herpes simplex around the mouth, mouth sores, acne. It helps to heal wounds such as athlete's foot, burns, dandruff, and relieve itching. According to a preferred embodiment of the present invention, the tea tree leaf oil is included in an amount of 0.5 to 1.5 wt% based on the total weight %.

The Origanum Majorana Flower Oil is a natural oil that helps digestion, purifies the skin, soothes, and relieves muscle pain. It is rich in vitamin A and vitamin C to protect eyesight, improve night blindness, prevent colds, It is effective for skin care and fatigue. According to a preferred embodiment of the present invention, the marjoram flower oil is included in an amount of 0.15 to 1.0 wt% based on the total weight %.

The sented geranium oil (Pelargonium Graveoles Oil) is involved in purifying and keeping the skin clean by regulating the secretion of sebum from oily skin, and helps to revitalize the skin by giving elasticity to the skin. In addition, it is involved in controlling sebum in oily scalp and promoting scalp circulation, helping to manage healthy scalp and hair. According to a preferred embodiment of the present invention, the sented geranium oil is included in an amount of 0.15 to 1.0 wt% based on the total weight %.

The orange flower oil (Citrus Aurantium Dulcis (Orange) Flower Oil) is composed of monoterpene-based main chemical components such as limonene, pinene, and myrcene, and relieves tension and anxiety caused by stress, and stimulates the antimicrobial effect and circulation for colds. It helps prevent and relieve swelling. In addition, it stabilizes the stomach, helps relieve digestive problems, and promotes appetite. In addition, it has the effect of discharging waste products, which relieves symptoms of acne and oily skin, as well as moisturizing and softening the skin, which is beneficial for dry and aged skin. According to a preferred embodiment of the present invention, the orange flower oil is included in an amount of 0.1 to 1.0 wt% based on the total weight %.

The chamomile flower oil (Anthemis Nobilis Flower Oil) stabilizes the mind and body, is effective against insomnia, and makes the skin soft and elastic. It also helps with headache, migraine, neuralgia, toothache and menstrual disorders in women. According to a preferred embodiment of the present invention, the chamomile flower oil is included in an amount of 0.05 to 1.0 wt% based on the total weight %.

The common jasmine flower oil (Jasminum Officinale Flower Oil) is involved in regulating skin balance by helping proper moisturizing power and secreting sebum, thereby normalizing the skin condition and helping to relieve skin stress. According to a preferred embodiment of the present invention, the common jasmine flower oil is included in an amount of 0.05 to 0.5 wt% based on the total weight %.

On the other hand, if the oil component content as described above is added in less than a small wt% outside the specified content range, not only the efficacy of each oil is exerted, but there is a concern that it may cause skin-related side effects. If it is added in excess of a large amount of wt%, the efficacy of each oil is not appropriate and only the characteristics of any one oil are remarkable, making it difficult to achieve the desired effect.

As a further embodiment according to the present invention, apricot seed oil (Prunus Armeniaca (Apricot) Kernel Oil) 20.0 to 52.0 wt%, jojoba seed oil (Simmondsia Chinensis (Simmondsia Chinensis ( Jojoba) Seed Oil) 25.0 to 35.0 wt%, Evening Primrose Oil (Oenothera Biennis (Evening Primrose) Oil) 20.0 to 30.0 wt%, Juniperus Communis Fruit Oil 0.5 to 3.0 wt%, Lavender Flower Oil (Lavandula Spica ( Lavender) Flower Oil) 0.5 to 3.0 wt%, Orange Peel Oil (Citrus Aurantium Dulcis (Orange) Peel Oil) 1.0 to 3.0 wt%, Tea Tree Leaf Oil (Melaleuca Alternifolia (Tra Tree) Leaf Oil) 0.5 to 1.5 wt%, Marjoram Flower Oil (Origanum Majorana Flower Oil) 0.15 to 1.0 wt%, Cented Geranium Oil (Pelargonium Graveoles Oil) 0.15 to 1.0 wt%, Orange Flower Oil (Citrus Aurantium Dulcis (Orange) Flower Oil) 0.1 to 1.0 wt%, Chamomile After adding 0.05 to 1.0 wt% of Anthemis Nobilis Flower Oil and 0.05 to 0.5 wt% of Jasminum Officinale Flower Oil, heating to 40-50°C and stirring under reduced pressure of 300-400 mmHg were performed. can do.

In addition, as a further embodiment according to the present invention, the composition for regenerating skin and alleviating atopic dermatitis may include a dispersant.

The further inclusion of a dispersant in the composition is to double the desired effect by properly blending between oils.

The dispersant is preferably composed of one or a combination of two or more selected from the group consisting of lecithin, organic acid monoglyceride, sorbitan fatty acid ester, polyoxyethylene fatty acid ester, and sorbitan stearate, but is not limited thereto.

The composition for skin regeneration and atopic dermatitis relief according to a further embodiment of the present invention is not particularly limited in its formulation, and conventional cosmetics, for example, skin adhesive-type cosmetic formulations, solutions, emulsions, suspensions, Ointments, lotions, gels, creams, powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations, sprays, solids, etc. may be formulated, but is not limited thereto. More specifically, serum, nutritional cream, massage cream, eye cream, cleansing cream, flexible cosmetics, converging cosmetics, nutritional lotion, cleansing foam, cleansing water, mask pack, spray, powder.

In addition, other ingredients in addition to the ingredients according to an embodiment of the present invention may be added to the composition for skin regeneration and atopic dermatitis relief of each formulation, depending on the formulation or purpose of use of other cosmetics. For example, the composition for regenerating skin and alleviating atopic dermatitis according to the present invention may add a base component to which a generally known cosmetic composition for skin administration can be blended, if necessary.

As an embodiment, when the formulation of the composition for skin regeneration and atopic dermatitis relief according to the present invention is a paste, cream or gel, vegetable oil, animal oil, paraffin, wax, tracant, starch, cellulose derivative, bentonite as a carrier component , Polyethylene glycol, silica, zinc oxide or talc, titanium oxide, etc. may be added.

When the formulation of the composition for skin regeneration and atopic dermatitis relief according to the present invention is a body gel, triethanolamine, menthol, glycerin, disodium EDTA, ethanol, carbomer, which are components generally used in the manufacture of body gel, Methylparaben, flavoring, and the like may be added.

Here, the triethanolamine and carbomer act as a thickening agent, menthol acts as a fragrance component, ethanol functions as a refreshing sensation, glycerin acts as a moisturizer, and disodium IDTA is a metal component chelate It is possible, and methylparaben is added to function as a preservative.

In addition to the specific ingredients described above, in order to achieve the functions of a thickening agent, a refreshing sensation, a fragrance ingredient, a moisturizer, etc., a composition for skin regeneration and atopic dermatitis relief according to the present invention was applied by applying known ingredients widely used in the art. Manufacturing may also be included in the scope of the present invention.

On the other hand, when the formulation of the composition for skin regeneration and atopic dermatitis relief according to the present invention is a powder or spray (aerosol), silica, lactose, talc, aluminum hydroxide, polyamide powder or calcium silicate may be added as a carrier component. In particular, in the case of a spray, a propellant such as chlorofluorohydrocarbon, propane/butane or dimethyl ether may be additionally included.

When the formulation of the composition for skin regeneration and atopic dermatitis relief according to the present invention is a solution or emulsion, a solvent, an emulsifying agent or a solubilizing agent is added as a carrier component, examples of which include water, ethanol, ethyl acetate, ethyl carbonate, Isopropanol, benzyl alcohol, propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol, or fatty acid ester of sorbitan.

When the formulation of the composition for skin regeneration and atopic dermatitis relief according to the present invention is a suspension, a liquid diluent such as water, ethanol or propylene glycol, ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxy Suspensions such as ethylene sorbitan ester, microcrystalline cellulose, aluminum metahydroxide, bentonite, tracanth or agar, and the like may be added.

When the formulation of the composition for skin regeneration and atopic dermatitis relief according to the present invention is a surfactant-containing cleansing, as a carrier component, aliphatic alcohol ether sulfate, aliphatic alcohol sulfate, isethionate, sulfosuccinic acid monoester, imidazolinium derivative, Methyl taurate, sarcosinate, fatty acid amide ether sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, linoline derivative or ethoxylated glycerol fatty acid ester, and the like may be added.

In addition, the composition for skin regeneration and atopic dermatitis relief according to the present invention can be prepared as a composition such as body gel, body lotion, body cream, body oil, etc. for promoting fat decomposition by adding conventional additives. Can also be manufactured. In this case, the composition for skin regeneration and atopic dermatitis relief according to the present invention is preferably used as a method of transdermal administration by applying or spraying directly on the skin.

In addition, the amount of the composition for regenerating skin and alleviating atopic dermatitis according to the present invention may be appropriately adjusted in consideration of individual differences, formulation, and shape such as age and skin fat condition, and according to an embodiment of the present invention The composition for skin regeneration and atopic dermatitis relief can be usefully used as cosmetics, pharmaceuticals or quasi-drugs for skin soothing.

In the following, the operational effects according to the configuration of the present invention will be described in more detail through Examples and Experimental Examples.

[Example]

With respect to the total weight, apricot seed oil (Prunus Armeniaca (Apricot) Kernel Oil) 39.2wt%, jojoba seed oil (Simmondsia Chinensis (Jojoba) Seed Oil) 28.0wt%, evening primrose oil (Oenothera Biennis (Evening Primrose) Oil) 22.5wt% %, Juniperus Communis Fruit Oil 1.5wt%, Lavandula Spica (Lavender) Flower Oil 2.2wt%, Orange Peel Oil (Citrus Aurantium Dulcis (Orange) Peel Oil) 2.2wt%, Tea Tree Leaf Oil (Melaleuca Alternifolia (Tra Tree) Leaf Oil) 1.1 wt%, Origanum Majorana Flower Oil 1.0 wt%, Cented Geranium Oil (Pelargonium Graveoles Oil) 0.9 wt%, Orange Flower Oil (Citrus Aurantium Dulcis ( Orange) Flower Oil) 0.9wt%, chamomile flower oil (Anthemis Nobilis Flower Oil) 0.3wt%, and common jasmine flower oil (Jasminum Officinale Flower Oil) 0.2wt% to prepare a liquid composition.

ingredient Content (wt%) Apricot Seed Oil (Prunus Armeniaca (Apricot) Kernel Oil) 39.2 Jojoba Seed Oil (Simmondsia Chinensis (Jojoba) Seed Oil) 28.0 Evening Primrose Oil (Oenothera Biennis (Evening Primrose) Oil) 22.5 Juniperus Communis Fruit Oil 1.5 Lavandula Spica (Lavender) Flower Oil 2.2 Orange Peel Oil (Citrus Aurantium Dulcis (Orange) Peel Oil) 2.2 Tea Tree Leaf Oil (Melaleuca Alternifolia (Tea Tree) Leaf Oil) 1.1 Origanum Majorana Flower Oil 1.0 Scented Geranium Oil (Pelargonium Graveolens Oil) 0.9 Orange Flower Oil (Citrus Aurantium Dulcis (Orange) Flower Oil) 0.9 Chamomile Flower Oil (Anthemis Nobilis Flower Oil) 0.3 Jasminum Officinale Flower Oil 0.2

[Experimental Example 1] Evaluation of skin epidermal cell regeneration ability

In order to evaluate the skin epidermal cell regeneration ability of the composition prepared in the above example, an in vitro scratch assay was used.

HaCaT cells (Keratinocytes) were cultured in DMEM cell culture solution containing 10% FBS at 37°C and 5% CO ₂ conditions, and then spread on a 6-well plate to become 1×10 ⁶ cells per well, and then cultured for 12 hours. The cells were completely attached. Scratcher (SPL) was used to scrape the center of the plate, and the compositions prepared in the examples were treated at a concentration of 50, 100, and 200 µg/ml, respectively, for 24 hours, and then the reduction of the scratched area was shown in %.

1 shows the results of in vitro scratch assay of HaCaT cells (Keratinocytes) for evaluating the regeneration ability of skin epidermal cells of the composition prepared in the above example.

Referring to FIG. 1, it was observed that treatment of HaCaT cells by concentration (50, 100, 200㎍/㎖) of the composition prepared in Example has an effect on regeneration of skin epidermal cells, compared with Adenosine, a positive control. It can be seen that similar or highly observed, it can be seen that the composition prepared in the Examples has an effect on the regeneration ability of skin epidermal cells.

[Experimental Example 2] Evaluation of skin dermal cell regeneration ability

In order to evaluate the skin dermal cell regeneration ability of the composition prepared in the above example, an in vitro scratch assay was used.

HDF cells (Human Dermal Fibroblast) were cultured in DMEM cell culture solution containing 10% FBS at 37°C and 5% CO ₂ conditions, and then spread on a 6-well plate to become 5×10 ⁵ cells per well, and then for 12 hours. The cells were completely attached while culturing. Scratcher (SPL) was used to scrape the center of the plate, and the compositions prepared in the examples were treated at concentrations of 50, 100, and 200 µg/ml, respectively, for 7 hours, and then the reduction of the scratched area was shown in %.

Figure 2 shows the results of the in vitro scratch assay of HDF cells (Human Dermal Fibroblast) for evaluating the skin dermal cell regeneration ability of the composition prepared in the above Example.

Referring to FIG. 2, it was observed that treatment of HaCaT cells by concentration (50, 100, 200 µg/ml) of the composition prepared in Example has an effect on regeneration of skin dermal cells, compared with Adenosine, a positive control. It can be seen that similar or highly observed, it can be seen that the composition prepared in the examples has an effect on the regeneration ability of skin dermal cells.

[Experimental Example 3] Evaluation of β-hexosaminidase secretion inhibitory ability

In order to evaluate the inhibitory effect on degranulation of the composition prepared in the above example, a β-hexosaminidase release assay was used.

RBL-2H3 cells (rat basophilic leukemia cells) were cultured in DMEM cell culture solution containing 10% FBS at 37°C and 5% CO ₂ conditions, and then spread on a 96-well plate to become 2×10 ⁴ cells per well. Cells were completely attached while incubating for 12 hours. After washing the cells of each well twice with Siraganian buffer (biosolution), each well was treated with Siraganian buffer and the composition prepared in Example to a final concentration of 25, 50, 100, 200 ㎍ / ㎖, and cultured for 1 hour. I did. Thereafter, the culture was further cultured for 3 hours under stimulation of PMA (50nM) and A23187 (1㎛). After the reaction was terminated on ice, 50 µl of the supernatant was transferred to a new 96 well plate, and 200 µl of 0.1% Triton X-100 was added to Pellet, dissolved, and 50 µl was transferred. In a 96 well plate containing the supernatant and pellet, 50 µl of substate buffer (1mM 4-ρ-Nitrophenyl-N-acetyl-β-D-glucosaminide, 0.05M sodium citrate, pH 4.5) was added and reacted at 37°C for 1 hour. Then, 100 µl of Stop solution (0.05M sodium carbonate buffer, pH 10) was added to terminate the reaction, and the absorbance was measured at 405 nm using a microplate reader. The secretion rate of β-hexosaminidase was expressed as a percentage after dividing the value of the supernatant by the value of the supernatant and the value of Pellet.

Figure 3 shows the suppression of β-hexosaminidase secretion to show the atopic dermatitis inhibitory effect of the composition prepared in the above example.

Referring to FIG. 3, the treatment by concentration (25, 50, 100, 200㎍/㎖) of the composition prepared in the Example was observed to have an effect of inhibiting the secretion of β-hexosaminidase, as compared to the positive control Dexamethasone. As observed similarly or highly, it can be confirmed that the composition prepared in Examples has an effect of inhibiting atopic dermatitis.

[Experimental Example 4] Histamine secretion inhibition ability evaluation

In order to evaluate the inhibitory effect on degranulation of the composition prepared in the above example, the amount of Histamine secretion was measured.

RBL-2H3 cells (rat basophilic leukemia cells) were cultured in DMEM cell culture solution containing 10% FBS at 37°C and 5% CO ₂ conditions, and then spread on a 96-well plate to become 2×10 ⁴ cells per well. Cells were completely attached while incubating for 12 hours. The composition prepared in Example was treated to a final concentration of 200 µg/ml, and cultured for an additional hour, and then the culture supernatant was separated. Histamine content was measured in the separated culture supernatant. Histamine content was measured using a Histamine ELISA kit (MYBioSource), and the concentration of Histamine was calculated from a standard curve calculated from the standard solution included in the kit.

4 shows the suppression of Histamine secretion to show the atopic dermatitis inhibitory effect of the composition prepared in the above example.

4, the treatment of the composition prepared in Example was observed to have an inhibitory effect on the secretion of Histamine, and as it was observed similarly compared to Dexamethasone, a positive control, atopic properties were observed by the composition prepared in Example. It can be confirmed that it has a dermatitis inhibitory effect.

[Experimental Example 5] Evaluation of antioxidant effect

In order to evaluate the antioxidant effect of the composition prepared in the above example, the free radical scavenging ability of DPPH was confirmed. To 200 μm DPPH (1,1-diphenyl-2-picryl hydrazyl) 200 μm dissolved in methanol, 50 μl of the composition prepared in Examples diluted with 1, 5, and 10% was added and reacted at 37° C. for 30 minutes. The absorbance was then measured at 405 nm using a microplate reader. The measured absorbance was expressed as the rate of decrease in absorbance of the composition-treated and untreated groups prepared in Examples.

Figure 5 shows the free radical scavenging ability of DPPH to show the antioxidant effect of the composition prepared in the above example.

Referring to FIG. 5, as it is observed that treatment by concentration (1%, 5%, 10%) of the composition prepared in the Example increases the free radical scavenging ability of DPPH, antioxidants by the composition prepared in the Example You can see that it works.

[Experimental Example 6] Fragrance Component Analysis

① Extraction of volatile fragrance components by solid trace extraction method

A method in which volatile organic compounds are adsorbed by distribution between the sample and the fibers in the headspace of the aqueous solution using a fixed bed coated with polydimethyl siloxane on the fiber, and the fiber adsorbed with the organic compounds is injected into the injector of Gas Chromatography (GC) to thermally desorb. As, after taking each component constituting the composition of the present invention and maintaining it at 75 ± 5 ℃,

SMPE (50/30㎛ divinylbenzene/carboxen/polydimethylsiloxane (DVB/CAR/PDMS) was vertically erected, and the volatile components in the sample were adsorbed to the fiber for 30 minutes, and then the adsorbed fragrance components were desorbed in a GC-MS injector for analysis. .

② Establishment of retention index (RI)

In order to obtain the retention index (RI), 40 mg/ℓ of an n-alkane mixed standard substance (C8-C40) was set to the same optimum conditions as the volatile fragrance component analysis and analyzed by GCMS (GCMS-QP2010, shimadzu, japan). After analyzing the retention time (RT) of the n-alkane standard substance, by applying a calculation formula, the retention index (RI) of each component compared to the analyzed component's retention time was established and identified for the identified component. Reliability was verified.

③ Analysis of volatile fragrance components

The extracted fragrance components were analyzed by GC/MS, and the analysis of the components of each peak separated in the total ionization chromatogram (TIC) was compared with the spectrum of the mass spectrum library (FFNSC 2, NIST 12, WILEY 7) and the mass spectral data book. It was confirmed by comparing the analysis data of the matched standard.

Meanwhile, the chromatogram analyzed by GC/MS after extracting the volatile aroma components of the composition of the present invention using the solid trace extraction method is shown in FIG. 6, and the qualitatively analyzed compounds are shown in Tables 2 to 5 below. . A total of 46 types of fragrance components were identified, and Limonene accounted for the largest proportion of about 20% of all volatile fragrance components, and terpene-based phytoncide substances represented by 3-Carenen and 4-Terpineol were also detected in the same high range. Became.

NO RI COMPOUND NAME MF MW Mg/kg One 932 α-Pinene C ₁₀ H ₁₆ 136 4.34 2 947 α-Fenchene C ₁₀ H ₁₆ 136 0.79 3 972 Sabinene C ₁₀ H ₁₆ 136 1.67 4 976 β-Pinene C ₁₀ H ₁₆ 136 0.44 5 989 β-Myrcene C ₁₀ H ₁₆ 136 3.79 6 1005 α-Phellandrene C ₁₀ H ₁₆ 136 0.31 7 1008 3-Carene C ₁₀ H ₁₆ 136 22.88 8 1016 α-Terpinene C ₁₀ H ₁₆ 136 3.80 9 1024 ρ-Cymene C ₁₀ H ₁₄ 134 3.33 10 1030 Limonene C ₁₀ H ₁₆ 136 46.39

NO RI COMPOUND NAME MF MW Mg/kg 11 1031 β-Phellandrene C ₁₀ H ₁₆ 136 0.96 12 1032 Eucalyptol C ₁₀ H ₁₈ O 154 1.04 13 1047 trans-β-Ocimene C ₁₀ H ₁₆ 136 1.57 I.S. 1056 n-Butylbenzene C ₁₀ H ₁₄ 134 0.00 14 1058 γ-Terpinene C ₁₀ H ₁₆ 136 6.38 15 1071 trans-Sabinene hydrate C ₁₀ H ₁₈ O 154 6.38 16 1085 Terpinolene C ₁₀ H ₁₆ 136 2.96 17 1100 Linalool C ₁₀ H ₁₈ O 154 17.01 18 1107 1-Octenyl-3-acetate C ₁₀ H ₁₈ O ₂ 170 0.59 19 1113 Phenylethyl Alcohol C ₈ H ₁₀ O 122 17.45

NO RI COMPOUND NAME MF MW Mg/kg 20 1125 1-Terpineol C ₁₀ H ₁₈ O 154 17.01 21 1163 Benzyl acetate C ₉ H ₁₀ O ₂ 150 0.38 22 1166 Isomenthone C ₁₀ H ₁₈ O 154 0.33 23 1174 Borneol C ₁₀ H ₁₈ O 154 0.26 24 1182 4-Terpineol C ₁₀ H ₁₈ O 154 18.89 25 1196 α-Terpineol C ₁₀ H ₁₈ O 154 2.45 26 1224 Nerol C ₁₀ H ₁₈ O 154 0.64 27 1227 Citronellol C ₁₀ H ₂₀ O 156 6.55 28 1249 Linalyl acetate C ₁₂ H ₂₀ O ₂ 196 17.33 29 1251 Geraniol C ₁₀ H ₁₈ O 154 11.48

NO RI COMPOUND NAME MF MW Mg/kg 30 1268 Citral C ₁₀ H ₁₆ O 152 0.24 31 1273 Citronellyl formate C ₁₁ H ₂₀ O ₂ 184 0.33 32 1282 Lavandulyl acetate C ₁₂ H ₂₀ O ₂ 196 1.92 33 1297 Geranyl formate C ₁₁ H ₁₈ O ₂ 182 0.30 34 1342 Methyl anthranilate C ₈ H ₉ NO ₂ 151 1.41 35 1357 Neryl acetate C ₁₂ H ₂₀ O ₂ 196 0.55 36 1377 Geranyl acetate C ₁₂ H ₂₀ O ₂ 196 1.28 37 1423 trans-Caryophyllene C ₁₅ H ₂₄ 204 1.76 38 1474 Cadina-1(6), 4-diene C ₁₅ H ₂₄ 204 1.14 39 1495 cis-Muurola-4(14), 5-diene C ₁₅ H ₂₄ 204 1.09 40 1520 γ-Cadinene C ₁₅ H ₂₄ 204 1.51 41 1524 Cadina-1,3,5-triene C ₁₅ H ₂₂ 202 0.59 42 1525 Epizonarene C ₁₅ H ₂₄ 204 0.50

Claims (5)

Apricot seed oil (Prunus Armeniaca (Apricot) Kernel Oil) 39.2wt%,
Jojoba seed oil (Simmondsia Chinensis (Jojoba) Seed Oil) 28.0wt%,
Evening Primrose Oil (Oenothera Biennis (Evening Primrose) Oil) 22.5wt%,
Juniperus Communis Fruit Oil 1.5wt%,
Lavandula Spica (Lavender) Flower Oil 2.2wt%,
Orange Peel Oil (Citrus Aurantium Dulcis (Orange) Peel Oil) 2.2wt%,
Tea Tree Leaf Oil (Melaleuca Alternifolia (Tra Tree) Leaf Oil) 1.1wt%,
1.0wt% of Origanum Majorana Flower Oil,
Scented Geranium Oil (Pelargonium Graveoles Oil) 0.9wt%,
Orange Flower Oil (Citrus Aurantium Dulcis (Orange) Flower Oil) 0.9wt%,
Anthemis Nobilis Flower Oil 0.3wt%,
The composition composed of 0.2wt% of Common Jasminum Officinale Flower Oil was heated to 40-50℃ in a reduced pressure stirrer, and then stirred under reduced pressure of 300-400mmHg, and then lecithin and organic acid monoglyceride were added to the composition. Ride, sorbitan fatty acid ester, polyoxyethylene fatty acid ester, and sorbitan stearate. A composition for skin regeneration and atopic dermatitis alleviation, characterized in that it further comprises a dispersant that is a combination of two or more selected from the group consisting of. delete delete delete delete

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