KR20190001530A - Composition for relieving and improving atopic dermatitis - Google Patents

Abstract

The present invention relates to a composition for relieving and curing atopic dermatitis, capable of relieving symptoms of atopic dermatitis by activating antibacterial action and antioxidant action and doubling skin moisturizing power by blending natural oil. The composition for relieving and curing atopic dermatitis also enables emotional healing by fragrance emitted by the natural oil. According to the present invention, the composition for relieving and curing atopic dermatitis is composed of: 70-95 wt% of base oil composed of 47.0-54.0 wt% of jojoba oil, 23.0-26.5 wt% of calendula oil, and 23.0-26.5 wt% of sweet almond oil; and 5-30 wt% of essential oil composed of 0.44-13.9wt% of chamomile oil, 0.75-1.76 wt% of jasmine oil, 17.5-19.38 wt% of juniper berry oil, 17.5-19.38 wt% of lavender oil, 17.5-22.02 wt% of sweet orange oil, 8.85-10.57 wt% of tea tree oil, and 24.0-26.45 wt% of Chamaecyparis obtusa oil.

Description

Technical Field [0001] The present invention relates to compositions for relieving atopic dermatitis and improving atopic dermatitis.

The present invention relates to a method for improving atopic dermatitis by activating antimicrobial and antioxidative action, improving the symptoms of atopic dermatitis, doubling the skin moisturizing power through blending of natural oils, and at the same time reducing atopic dermatitis, And to a composition for improvement.

Atopic dermatitis is characterized by a decrease in barrier function of epidermis such as increase of globulin E, increase of lymphocyte, increase of mast cell, increase of pH, increase of transdermal water loss, decrease of ceramide due to expansion of cells and cells Feature.

Atopic dermatitis is also called enthusiasm and is accompanied by chronic allergic diseases such as asthma and hay fever. Allergies can also be considered as a factor that can cause atopic dermatitis. When the skin reaction test for the protein antigen is performed, the urticaria reaction to many antigens appears well. When the body is exposed to these antigen, Immediate responses such as anaphylactic shock, urticaria and angioedema, wheezing and dyspnea, nasal congestion, runny nose, sneezing, conjunctival injection and swelling, vomiting, diarrhea, And intestinal mucosa, and these inadequate responses are characteristic of family history.

Factors that can aggravate atopic dermatitis include dry skin, ambient temperature and humidity, skin irritation due to severe exercise and sweat, wool and fibers, food, drugs, house dust, animal hair, irritant chemicals, harmful bacterial infections Or mental stress. In addition, it has been reported that the germs and fungi present in the skin are exacerbated (Japanese Society of Allergy and Allergy, No. 381p), and bacteria and fungi on the skin are deeply involved in atopic dermatitis S. aureus and P. ovale (51st Japanese Society of Allergy, Lecture Book No. 20p).

These atopic dermatitis is recurrent chronic dermatitis accompanied by severe itching. It is reported that about 10 to 15% of children have atopic dermatitis, and about 90% of them develop on their own within 5 years. Approximately 30-40% are not recurring on appearance, but approximately 60-70% of the rest are known to cause severe dermatitis, such as skin irritation due to irritant substances, eczema, and skin irritation. The ratio is gradually increasing with increasing environmental pollution.

On the other hand, dry and sensitive skin has low water holding capacity and resilience, and thus it is prone to skin keratinization and itching, and in severe cases, it develops into skin diseases such as atopic dermatitis. Especially, when atopic fever does not spread, it causes skin rash and rash and itching. It can be said that it is a skin disease in which immune function is extremely decreased due to frequent use of steroid ointment and antihistamine.

Therefore, various drugs have been developed to improve the itching caused by atopic dermatitis and dryness of the skin. However, since most of these drugs are steroid drugs and antibiotics as active ingredients, Or to cause an increase in the thickness or expansion of the stratum corneum.

Due to the above problems, cosmetics containing natural ingredients such as white roots, cloves, gardenia, sculptors, and herbal teas have been developed to contain the least amount of chemical components. However, in order to extract the active ingredients, The preservative added to prevent the corruption of the extracted active ingredient had potential problems that could cause serious side effects on the skin of the human body.

Korean Patent Publication No. 10-2017-0061805 (2017.06.07. Composition for treating atopic dermatitis) Korean Patent Laid-Open No. 10-2011-0130555 (December, 2011. Method for manufacturing cream for prevention and improvement of atopic skin) Korean Patent No. 10-1724147 (May 31, 2017. Cosmetic composition for suppressing and alleviating itching including herbal extracts)

Disclosure of Invention Technical Problem [8] Accordingly, the present invention has been made to solve the above-mentioned problems occurring in the prior art, and it is an object of the present invention to alleviate and improve the symptoms of atopic dermatitis which is a skin disease by activating antibacterial and antioxidative action, It is another object of the present invention to provide a composition for alleviating and improving atopic dermatitis, which is capable of emotional healing due to the fragrance emitted from natural oils, and which does not cause side effects by using a material having safety and functionality secured from natural materials.

The composition of the present invention comprises a base composed of 47.0 to 54.0 wt% of jojoba oil, 23.0 to 26.5 wt% of calendula oil and 23.0 to 26.5 wt% of sweet almond oil, 70 to 95 wt% of a base oil; , Chamomile oil 0.44 - 13.9wt%, jasmine oil 0.75 - 1.76wt%, juniper berry oil 17.5 - 19.38wt%, lavender oil 17.5 - 19.38wt% Essential oil 5 composed of sweet orange oil 17.5 - 22.02 wt%, tea tree oil 8.85 - 10.57 wt% and chamaecyparis otbusa oil 24.0 - 26.45 wt% - 30 wt%.

In a further embodiment of the present invention, the composition comprises 0.1-5.0 parts by weight of at least one selected from animal oil, vegetable oil, wax, paraffin, starch 0.5 0.1 to 3.0 parts by weight of a cellulose derivative, 0.1 to 5.0 parts by weight of bentonite, silica, talc, zinc oxide 0.1 to 3.0 parts by weight of at least one component selected from the group consisting of zinc oxide and titanium oxide is mixed and applied to the skin in cream form.

In another embodiment of the present invention, the composition comprises 0.1 to 5.0 parts by weight of a carbomer, 0.1 to 1.0 part by weight of triethanolamine, 0.1 to 5.0 parts by weight of menthol, 0.1 to 5.0 parts by weight of ethanol, 0.1 to 10.0 parts by weight of glycerin, 0.1 to 10.0 parts by weight of disodium EDTA and 0.1 to 2.0 parts by weight of methylparaben are mixed to prepare a gel GEL). ≪ / RTI >

In still another embodiment of the present invention, the composition comprises 0.1 to 4.0 parts by weight of lactose, 0.1 to 2.0 parts by weight of talc or silica, 0.1 to 1.0 part by weight of aluminum hydroxide and 0.1 to 5.0 parts by weight of calcium silicate or polyamide powder alone or in the form of a powder is mixed in powder form And is applied to the skin.

In still another embodiment of the present invention, the composition may contain 0.1 to 10.0 parts by weight of purified water, 0.1 to 5.0 parts by weight of ethanol, 0.1 to 3.0 parts by weight of isopropanol, 0.1 to 3.0 parts by weight of ethyl carbonate, 0.1 to 5.0 parts by weight of ethyl acetate, 0.1 to 5.0 parts by weight of benzyl alcohol, 0.1 to 3.0 parts by weight of benzyl benzoate, 0.1 to 5.0 parts by weight of propylene glycol, 0.1 to 5.0 parts by weight of 1,3-butylene glycol oil, a fatty acid ester of polyethylene glycol or sorbitan (fatty acid ester) or 0.1 to 3.0 parts by weight of a mixed component thereof is mixed and applied to the skin in the form of an emulsion.

In still another embodiment of the present invention, the composition may contain 0.1 to 10.0 parts by weight of purified water, ethanol or propylene glycol alone or a mixture thereof 0.1 to 5.0 parts by weight, 0.1 to 3.0 parts by weight of ethoxylated isostearyl alcohol, 0.1 to 3.0 parts by weight of polyoxyethylene sorbitol ester, 0.1 to 5.0 parts by weight of cellulose, 0.1 to 4.0 parts by weight of aluminum metahydoxide, 0.1 to 3.0 parts by weight of bentonite, 0.1 to 2.0 parts by weight of agar or tragacanth alone or a mixed component thereof, Is applied to the substrate.

The composition for alleviating and improving atopic dermatitis according to the present invention having the above-

First, it alleviates and improves the symptoms of atopic dermatitis by activating antimicrobial and antioxidant action,

Second, since materials having safety and functionality secured from natural materials are used, there is no fear of side effects such as skin irritation and skin trouble,

Third, by blending natural oils, the skin moisturizing power can be doubled,

Fourth, there is an effect that emotional healing by the fragrance emitted from natural oil is possible.

FIG. 1 is a graph showing the DPPH radical scavenging activity of the composition for alleviating and improving atopic dermatitis according to a preferred embodiment of the present invention,
FIG. 2 is a graph showing the DPPH radical scavenging activity of essential oils constituting the composition for alleviating and improving atopic dermatitis according to a preferred embodiment of the present invention,
FIG. 3 is a graph showing the ABTS radical scavenging activity of the composition for alleviating and improving atopic dermatitis according to a preferred embodiment of the present invention,
FIG. 4 is a graph showing the ABTS radical scavenging activity of essential oils constituting the composition for alleviating and improving atopic dermatitis according to a preferred embodiment of the present invention,
FIG. 5 is a graph showing FRAP reduction power by mixing ratio of composition for mitigating and improving atopic dermatitis according to a preferred embodiment of the present invention,
FIG. 6 is a graph showing the results of evaluation of antimicrobial activity of Escherichia coli according to the mixing ratio of the composition for alleviating and improving atopic dermatitis according to a preferred embodiment of the present invention coli < / RTI > activity,
FIG. 7 is a photograph and table showing the measurement of the activity of Salmonella typhimurium according to the mixing ratio of the composition for alleviating and improving atopic dermatitis according to a preferred embodiment of the present invention,
FIG. 8 is a graph showing the effect of the antimicrobial activity of Candida albicans ) photos and tables,
FIG. 9 is a photograph and table showing the measurement of the activity of Staphylococcus aureus according to the mixing ratio of the composition for alleviating and improving atopic dermatitis according to a preferred embodiment of the present invention,
FIG. 10 is a graph comparing moisturizing effects of the composition for mitigating and improving atopic dermatitis according to a preferred embodiment of the present invention,
11 is a graph showing chromatogram results obtained by extracting fragrance components of a composition for alleviating and improving atopic dermatitis according to a preferred embodiment of the present invention by the SDE method,
12 is a graph showing chromatogram results obtained by extracting aroma components of a composition for alleviating and improving atopic dermatitis according to a preferred embodiment of the present invention by the SPME method,
FIG. 13 is a graph showing the chromatogram results obtained by extracting aroma components of a composition for alleviating and improving atopic dermatitis according to a preferred embodiment of the present invention by the SDE method and the SPME method,
FIG. 14 is a graph showing the RBL 2H3 cytotoxicity results of the composition for mitigating and improving atopic dermatitis according to a preferred embodiment of the present invention,
15 is a graph showing the results of β-hexosaminidase secretion of RBL 2H3 treated according to the mixing ratio of compositions for atopic dermatitis relief and improvement according to a preferred embodiment of the present invention.

The composition for alleviating and improving atopic dermatitis according to the present invention comprises:

70 to 95 wt% of a base oil consisting of 47.0 to 54.0 wt% of jojoba oil, 23.0 to 26.5 wt% of calendula oil and 23.0 to 26.5 wt% of sweet almond oil, Wow;

, Chamomile oil 0.44 - 13.9wt%, jasmine oil 0.75 - 1.76wt%, juniper berry oil 17.5 - 19.38wt%, lavender oil 17.5 - 19.38wt% Essential oil 5 composed of sweet orange oil 17.5 - 22.02 wt%, tea tree oil 8.85 - 10.57 wt% and chamaecyparis otbusa oil 24.0 - 26.45 wt% - 30wt%.

Table 1 shows the main characteristics of the respective materials.

Kinds Key Features Jojoba oil
(jojoba oil) It is rich in vitamins and minerals. It protects the skin as well as relieves skin troubles and moisturizes. Calendula oil
calendula oil It is effective for anti-inflammation, skin damage treatment, dry skin relief, antifungal, anti-inflammation, anti-aging, allergy relief, atopy Sweet almond oil
(sweet almond oil) Contains vitamins A and E, minerals and proteins, moisturizes dry skin, energizes and nourishes tired skin, enhances skin shine Chamomile oil
(chamomile oil) Anti-inflammatory, soothing, antioxidant, itching improvement, allergic eczema relief, acne skin soothing and antibacterial agent Jasmine oil
(jasmine oil) Relieve skin, relieve skin troubles, nourish skin and improve skin elasticity Juniper Berry Oil
(juniper berry oil) It is effective for skin moisturization, skin nourishment, skin radiance and vitality, skin soothing and anti-aging. Lavender Oil
(lavender oil) It is effective for skin nutrition, skin moisture retention, antibacterial, sebum suppression and exfoliation. Sweet orange oil
(sweet orange oil) It is effective for antifungal, sterilization, dry skin, inflammatory skin, acne skin soothing, skin regeneration and anti-aging. Tea tree oil
(tea tree oil) It is effective for skin soothing, sensitive skin, antibacterial, acne treatment and inflammation soothing. Linseed oil
(chamaecyparis otbusa oil) Phytoncide effect, natural antibacterial, concentration improvement, immune cell activity, skin disease improvement, pore care, sick house syndrome relaxation, active oxygen scavenging ability, elemol

Hereinafter, the critical significance of the upper and lower limits according to the composition ratios of the respective materials constituting the composition for alleviating and improving atopic dermatitis of the present invention will be described in detail.

(1) Jojoba oil: Liquid ester oil obtained from seeds of jojoba, which is superior in terms of stability and feel, and is widely used as an oil-based raw material mainly in basic cosmetics. Jojoba oil is less oily than other vegetable oils and has a wax ester structure, a component of sebum. Therefore, it is often used as an oil component in make-up products such as emulsions, creams, body oils, skin care products such as hand lotions, lipsticks, and foundations.

In the present invention, it is preferable that the jojoba oil having a structure of wax ester which is one component of sebum is contained in an amount of 47.0 to 54.0 wt% based on the total wt%. The reason for this is that when the content is less than 47.0 wt%, the effect of moisturizing and anti-aging is reduced. When the content is more than 54.0 wt%, there is a problem in formulation and it is difficult to use.

(2) Calendula oil: It is a leavening oil which puts marigold flower in sunflower oil. It heals and protects the skin and mucous membranes of wounded areas and exerts excellent functions on delicate skin. In addition, it is a natural oil that exerts excellent effects to treat cuts and eczema due to its excellent anti-inflammatory and antifungal action.

The calendula oil having the above characteristics is preferably contained in an amount of 23.0 to 26.5 wt% based on the total wt%. The reason for this is that when the content is less than 23.0 wt%, the functions such as anti-inflammation and antifungals are decreased. When the content is more than 26.5 wt%, the homogeneous mixture with other materials is not obtained.

(3) Sweet almond oil: Pale yellow oil extracted from almond seeds and has odorless properties. It contains a lot of nutrients, especially because it contains a large amount of protein, it is excellent in softening the skin. It also contains vitamins D, E and minerals to control the itchiness of the skin. It is very effective for dry skin, inflammatory diseases, and restores tired skin in a short time.

The sweet almond oil is preferably contained in an amount of 23.0 to 26.5 wt% based on the total wt%. The reason is that when it is contained less than 23.0 wt%, the resilience of inflammatory diseases and tired skin is lowered, as well as the viscosity is increased and there is a fear of solidification. When the content exceeds 26.5 wt%, the liquid state There is a concern that it may flow down immediately upon application to the skin, and it is accompanied with an inconvenience in use because of the increased slipperiness.

(4) chamomile oil: calms the mind and body, helps to insomnia, makes the skin soft and elastic. It is effective for relieving headache, migraine, neuralgia, toothache and menstrual cramps, and has few side effects. It is a natural oil that can be used for most allergic skin.

The above-mentioned chamomile oil is preferably contained in an amount of 0.44 to 13.9 wt% with respect to the total wt%. The reason for this is that when the content is less than 0.44 wt%, the effect such as pain relief is not exerted. When the content is more than 13.9 wt%, it is unnecessarily added, resulting in an uneconomical and high viscosity, Because.

(5) Jasmine oil: A sweet aroma creates a romantic mood and softens the mood. It also helps in the treatment of severe depression and stimulates energy to help you find vitality. Recent research has shown that women can improve their sexual dysfunction and improve the fertility of men by increasing the number of spermatozoa.

The jasmine oil is preferably contained in an amount of 0.75 to 1.76 wt% based on the total wt%. The reason for this is that when it is contained at less than 0.75 wt%, the intrinsic flavor of jasmine which is helpful for the treatment and vitality of depression is not diverted. When the content exceeds 1.76 wt%, the stability of the formulation is drastically decreased, This is because no mixing occurs.

(6) Juniper berry oil: It is effective for diet, urinary disinfection and cystitis, urination and kidney stones. In addition, it detoxifies to eliminate toxicity from carnivorous and overeating, normalizes the physiology of women, and relieves painful seizures.

It is preferable that the Juniper berry oil having the above characteristics is contained in an amount of 17.5 to 19.38 wt% based on the total wt%. If it is contained in an amount less than 17.5 wt%, not only the divergence of the aromatic material is small but also the effective ingredient such as alpha-pinene is canceled by the other substance, so that it is difficult to exhibit the above-mentioned functional characteristics, If it is contained, polyphenol, which is an active ingredient of juniper berry, sharply increases and accompanies side effects to the human body.

(7) Lavender oil: The main chemical components are ester type such as linalyl acetate, lavandulyl acetate and alcohol type such as linalool and geraniol. , A safe oil without toxicity and irritation, and is one of the most used oils in the aromatherapy field.

The lavender oil is preferably contained in an amount of 17.5 to 19.38 wt% based on the total wt%. This is because when the lavender oil is contained in an amount of less than 17.5 wt%, the skin soothing, moisturizing effect and anti-inflammatory effect are insignificant, and when it is contained in an amount exceeding 19.38 wt%, it is added unnecessarily and is uneconomical.

(8) Sweet orange oil: It relieves tension, helps to relieve chronic anxiety and depression, and helps relieve stress. Women's physiological pre-tension and menopausal symptoms such as excitement, loneliness, and also has the effect of improving anxiety. It is a natural oil that has excellent regenerating effect of skin cells and exerts good effects on dry skin, sensitive skin and aging skin.

The sweet orange oil having the above characteristics is preferably contained in an amount of 17.5 to 22.02 wt% based on the total wt%. The reason for this is that when it is contained less than 17.5 wt%, the regenerating effect of skin cells, which is one of the main features of sweet orange oil, is not sufficient. When the content exceeds 22.02 wt%, the stability is lowered during the formulation, , That is, mixing with other materials is not easy.

(9) Tea tree oil: tea tree oil has the advantage of activating the immune system to combat infectious diseases. It also activates leukocytes, has antibacterial and antifungal effects, and is effective against influenza, herpes, catarrh, and fever and gingivitis.

On the other hand, the tea tree oil having the above characteristics is preferably contained in an amount of 8.85 to 10.57 wt% with respect to the total wt%. If it is contained in an amount less than 8.85 wt%, the synergistic effect with lactic acid is not sufficient, and the skin is not sufficiently protected and moisturized. When it is contained in an amount exceeding 10.57 wt%, sticky discomfort And stimulation by a strong incense becomes severe.

(10) Chamaecyparis otbusa oil: Chamaecyparis otbusa oil has an excellent effect for the improvement of allergy and skin diseases. Especially, it is one of the main cause of atopy, and it has excellent antidumping effect of house dust mite. It neutralizes volatile organic compounds causing sick house syndrome . It also acts to alleviate skin itching, the most painful part of atopic and allergic diseases.

It is preferred that the linseed oil having such characteristics is contained in an amount of 24.0 to 26.45 wt% based on the total wt%. The reason for this is that when it is contained less than 24.0 wt%, it does not sufficiently cope with atopic or allergic diseases. When it is contained in excess of 26.45 wt%, it is unnecessary because it is contained more than necessary, It is because of the unpleasantness and the stimulation by strong incense.

The listed materials can be broadly divided into base oil and essential oil. The base oils include jojoba oil, calendula oil and sweet almond oil. Examples of essential oils include chamomile oil, jasmine oil, juniper berry oil, lavender oil, sweet orange oil, tea tree oil and linseed oil.

The mixing ratio of the base oil and the essential oil is preferably 70 to 95 wt% of the base oil and 5 to 30 wt% of the essential oil. This is because the composition mixed at the above ratios not only effectively inhibits degranulation, but also has excellent cytotoxicity-inhibiting ability.

In a further embodiment of the present invention, the composition may be formulated with creams, gels, powders, emulsions, suspensions or the like for application to the skin by adding additional components to the composition in which oils of natural origin are mixed to form a liquid state have.

As an example, the composition according to the present invention comprises 0.1-5.0 parts by weight of at least one selected from animal oil, vegetable oil, wax and paraffin, 0.5-2.5 parts by weight starch, 0.1 to 5.0 parts by weight of tragacanth, 0.1 to 3.0 parts by weight of a cellulose derivative, 0.1 to 5.0 parts by weight of bentonite, silica, talc, zinc oxide 0.1 to 3.0 parts by weight of at least one component selected from titanium oxide and the like can be mixed and applied to the skin in the form of a cream.

0.1 to 5.0 parts by weight of a carbomer, 0.1 to 1.0 part by weight of triethanolamine, 0.1 to 5.0 parts by weight of menthol, 0.1 to 5.0 parts by weight of ethanol, 0.1 to 10.0 parts by weight of glycerin, 0.1 to 10.0 parts by weight of disodium EDTA and 0.1 to 2.0 parts by weight of methylparaben are mixed to form a gel (GEL) have.

On the other hand, as an example of formulating the composition into a powder form, 0.1 to 4.0 parts by weight of lactose, talc, or silica alone or a mixture thereof 0.1 to 2.0 parts by weight of aluminum hydroxides, 0.1 to 1.0 part by weight of aluminum hydroxides, calcium silicate or polyamide powder alone or 0.1 to 5.0 parts by weight of the mixed components thereof .

0.1 to 10.0 parts by weight of purified water, 0.1 to 5.0 parts by weight of ethanol, 0.1 to 3.0 parts by weight of isopropanol, 0.1 to 3.0 parts by weight of ethyl carbonate, 0.1 to 5.0 parts by weight of ethyl acetate, 0.1 to 5.0 parts by weight of benzyl alcohol, 0.1 to 3.0 parts by weight of benzyl benzoate, 0.1 to 5.0 parts by weight of propylene glycol, 0.1 to 5.0 parts by weight of a 1,3-butylene glycol oil, a fatty acid ester of polyethylene glycol or sorbitan, or a mixture thereof 0.1 - 3.0 Or 0.1 to 10.0 parts by weight of purified water, 0.1 to 5.0 parts by weight of ethanol or propylene glycol alone or a mixture thereof, and 0.01 to 10 parts by weight of ethoxylated Isostearyl 0.1 to 3.0 parts by weight of ethoxylated isostearyl alcohol, 0.1 to 3.0 parts by weight of polyoxyethylene sorbitol ester, 0.1 to 5.0 parts by weight of cellulose, 0.1 to 4.0 parts by weight of aluminum metahydoxide, 0.1 to 3.0 parts by weight of bentonite, 0.1 to 3.0 parts by weight of agar or tragacanth, or 0.1 to 2.0 parts by weight of a mixed component thereof may be mixed to form a suspension and applied to the skin.

In addition to the above-described formulations, the composition for alleviating and improving atopy according to the present invention may be modified into various formulations to be applied to skin such as soap, ointment, patch, spray, mask pack, emulsion, aerosol and the like.

In addition to this, it is also within the scope of the present invention to produce a composition by applying known substances widely used in the art to add functions such as a thickening agent, a refreshing sensation, a fragrant ingredient, a moisturizing agent, etc. Of course.

The amount of the composition for alleviating and improving atopic dermatitis according to the present invention can be suitably adjusted according to individual differences, formulations, and forms such as age and condition of skin fat. It is also useful as a medicament or a quasi-drug Can be handled.

Hereinafter, the present invention will be described in more detail by way of examples, including examples.

[Example 1]

Base oil composed of 50% by weight of jojoba oil, 25% by weight of calendula oil and 25% by weight of sweet almond oil and 8% by weight of chamomile oil, 1% by weight of jasmine oil, 18% by weight of juniper berry oil, 18% by weight of lavender oil, Essential oil consisting of 20% by weight of orange oil, 10% by weight of tea tree oil and 25% by weight of plain white oil was prepared,

The base oil and the essential oil were mixed in a weight ratio of 9.5: 0.5 to prepare a sample of a blending oil to be used in the experiment.

[Example 2]

Base oil composed of 50% by weight of jojoba oil, 25% by weight of calendula oil and 25% by weight of sweet almond oil and 8% by weight of chamomile oil, 1% by weight of jasmine oil, 18% by weight of juniper berry oil, 18% by weight of lavender oil, Essential oil consisting of 20% by weight of orange oil, 10% by weight of tea tree oil and 25% by weight of plain white oil was prepared,

The base oil and the essential oil were mixed in a weight ratio of 9.0: 1.0 to prepare a sample of a blending oil to be used in the experiment.

[Example 3]

Base oil composed of 50% by weight of jojoba oil, 25% by weight of calendula oil and 25% by weight of sweet almond oil and 8% by weight of chamomile oil, 1% by weight of jasmine oil, 18% by weight of juniper berry oil, 18% by weight of lavender oil, Essential oil consisting of 20% by weight of orange oil, 10% by weight of tea tree oil and 25% by weight of plain white oil was prepared,

The base oil and the essential oil were mixed at a weight ratio of 8.5: 1.5 to prepare a sample of a blending oil to be used in the experiment.

[Example 4]

Base oil composed of 50% by weight of jojoba oil, 25% by weight of calendula oil and 25% by weight of sweet almond oil and 8% by weight of chamomile oil, 1% by weight of jasmine oil, 18% by weight of juniper berry oil, 18% by weight of lavender oil, Essential oil consisting of 20% by weight of orange oil, 10% by weight of tea tree oil and 25% by weight of plain white oil was prepared,

The base oil and the essential oil were mixed in a weight ratio of 8.0: 2.0 to prepare a sample of a blending oil to be used in the experiment.

[Experimental Example 1] Evaluation of antioxidative activity

(1) Measurement of DPPH radical scavenging ability

DPPH radical scavenging activity was measured using the oil samples prepared in Examples 1-4. The radical scavenging activity of Example 1 (base oil and essential oil composition ratio 9.5: 0.5) to 100 μl of a 0.1 mM DPPH solution dissolved in methanol was measured in the same manner as in Example 2 (Base oil and essential oil composition ratio 9.0: 1.0), Example 3 (base oil and essential oil composition ratio 8.5: 1.5) and Example 4 (base oil and essential oil composition ratio 8.0: 2.0) After adding 100 μl of star (25, 50, 100 and 200 μg / ml), the reaction was carried out in the dark room for 30 minutes and the absorbance was measured at 517 nm using a microplate reader. DPPH radical scavenging activity was calculated by the following equation.

Here, A _sample represents the absorbance of the sample and the DPPH reaction solution, A _blank1 represents the single absorbance of the sample, A _blind represents the single absorbance of the DPPH solution, and A _blank2 represents the blank sample.

As shown in the graph of FIG. 1, DPPH radical scavenging ability was increased in all the oil treatments in a concentration-dependent manner, but no difference in radical scavenging ability was observed by composition ratio.

In addition, the DPPH radical scavenging ability of each oil contained in the essential oils of Examples 1 to 4 was observed. The oils of seven essential oils (AN: chamomile oil, JO: jasmine oil, JC: Juniper berries oil, LA: lavender oil, CA: sweet orange oil, MA: tea tree oil, As shown in the graph shown in FIG. 2, the antioxidative activity was highest in chamomile oil, and jasmine oil, juniper oil, Berry oil, tea tree oil, and white rice oil.

(2) Measurement of ABTS radical scavenging ability

The ABTS radical scavenging activity was measured using the oil samples prepared in Examples 1-4. (ABTS, Sigma-Aldrich Co.) were incubated for 24 hours with 7.4 mM ABTS and 2.6 mM potassium persulfate (Sigma-Aldrich Co.) in a solution of 2,2'-azine-bis- (3-ethylbenzothiazoline- After cows were left standing to form ABTS radicals, the solution was diluted with distilled water to an absorbance value of 1.5 at 760 nm. Example 1 (base oil and essential oil composition ratio 9.5: 0.5), Example 2 (base oil and essential oil composition ratio 9: 1), and Example 3 (base oil and essential oil composition (25, 50, 100 and 200 占 퐂 / ml) prepared in Example 4 (base oil and essential oil composition ratio of 8: 2) After 1 ml of each sample (62.5, 125, 250, 500 and 1,000 μg / ml) was treated, absorbance was measured at 760 nm using a microplate reader. The ABTS radical scavenging activity was calculated by the following formula.

Here, A _sample represents the absorbance of sample and ABTS reaction solution, and A _blind represents the single absorbance of ABTS solution.

As can be seen from the graph shown in FIG. 3, the ABTS radical scavenging ability was increased in all the oil treatments in a concentration-dependent manner, but no difference in radical scavenging ability was observed by the composition ratio.

In addition, the ABTS radical scavenging activity of each oil contained in the essential oils of Examples 1 to 4 was observed. The oils of seven essential oils (AN: chamomile oil, JO: jasmine oil, JC: Juniper berries oil, LA: lavender oil, CA: sweet orange oil, MA: tea tree oil, As shown in the graph shown in FIG. 4, the results of observation with respect to the ABTS radical scavenging ability by treatment with the concentrations (25, 50 and 100 μg / ml) showed that the antioxidative activity of chamomile oil was the highest , Jasmine oil, juniper berry oil, tea tree oil, and white tea oil.

(3) Measurement of FRAP reduction power

FRAP reducing power was measured using the oil samples prepared in Examples 1 to 4. 100 [mu] l / 100 [mu] g / ml) of the composition prepared by varying the base oil and essential oil composition ratios (9.5: 0.5, 9.0: 1.0, 8.5: 1.5, 8.0: 2.0) 100 μl of 0.2 M sodium phosphate buffer (pH 6.6) was added to each well, and the mixture was reacted in a constant-temperature water bath at 50 ° C. for 20 minutes. Then, 100 μl of 10% trichloroacetic acid (Sigma-Aldrich Co.) was added and centrifuged at 12,000 rpm for 10 minutes . Subsequently, 20 μl of 0.1% ferric chloride (Sigma-Aldrich Co.) was added, reacted in a constant temperature water bath at 37 ° C for 20 minutes, and the reducing power was measured at 700 nm using a microplate reader.

As shown in the graph of FIG. 5, the reducing power of inhibition against iron oxidation in the iron oxidation (Fe ^{2 +} → Fe ^{3 +} ) model accelerated oxidation by composition ratio was measured. As a result, the radical scavenging ability of DPPH and ABTS Similar to the results of the composition ratio.

[Experimental Example 2] Evaluation of antibacterial activity

The antibacterial activity was measured using the oil samples prepared in Examples 1 to 4. As the strain used in the experiment are bacterial vaginosis (Candidia albicans , Staphylococcus aureus , E. coli , and Salmonella typhimurium . The strains were cultured under the conditions shown in Table 2 below.

turn Microorganism name Use badge Growing conditions Remarks One Escherichia coli
Escherichia Coli EMB Agar
(Eosine Methylene Blue Agar) 35-37 ℃, aerobic food poisoning 2 Candidas
Candida albicans PDA
(Potato Dextrose Agar) 25-30 ° C, flowable anaerobic vaginitis 3 Staphylococcus aureus
Staphylococcus
aureus TSA
(Tryptic strain Agar) 35-37 ℃, aerobic Purulent dermatitis,
food poisoning 4 Salmonella
Salmonella
typhimurium SS agar
(Salmonella-Shigella agar) 35-37 ℃, aerobic food poisoning

Candida The size of the rings produced by the disc method to measure the antimicrobial activity of the oil samples prepared in Examples 1 to 4 for albicans , Staphylococcus aureus , E. coli , Salmonella typhimurium , Were measured. For the analysis of antimicrobial activity, each microorganism was first cultured at a concentration of 1 × 10 ¹⁰ CFU / ml according to the optimal culture conditions and cultured conditions, and then 100 μl was plated on the respective solid medium cultured for each microorganism. Then, a paper disc having a diameter of 8 mm was placed on a sterilized sterilized medium, and 50 쨉 l of the mixed composition was loaded at the respective composition ratios. The antimicrobial activity was measured by measuring the diameter (mm) of the clear zone after culturing the solid medium in an incubator for 48 to 72 hours at the optimum temperature.

Experimental results As shown in FIG. 6 to 9, the composition according to the invention was observed to be very high antibacterial activity in Escherichia coli (E. coli), Salmonella (Salmonella typhimurium) group, bacterial vaginosis (Candidia albicans ) and Staphylococcus aureus ( Staphylococcus aureus ). Further, no difference in antimicrobial effect of the composition by different composition ratios was observed.

[Experimental Example 3] Evaluation of moisturizing effect

Moisture resistance was measured using the oil samples prepared in Examples 1 to 4. In the experimental method, a square having a width of 10 mm and a length of 10 mm was displayed between the elbow and the fingertip of the subject, and 100 μl of each sample was applied to the marked area. The moisture content was measured, and the moisture retention for 8 hours was compared based on the measured value. Moisture resistance was measured with Combine CM820-SM820 PH900 (Courage + Khazaka) skin moisture meter and the procedure was measured once a day , And the average skin moisture content was measured by repeating the experiment three times. Table 3 below shows a list of samples for skin moisturizing measurement.

No Sample Code Name Sample Name One None treat Control (no treatment group) 2 CO-1 base: essential oil ratio (8.0: 2.0) 3 CO-2 base: essential oil ratio (8.5: 1.5) 4 CO-3 base: essential oil ratio (9.0: 1, 0) 5 CO-4 base: essential oil ratio (9.5: 0.5) 6 SCJO Oil mix 1 (positive control) 7 SCO Oil mix 2 (positive control)

On the other hand, as shown in FIG. 10, the skin moisturizing test results of each sample showed that the composition having the composition ratio of CO-3, that is, base oil and essential oil of 9.0: 1.0, showed the highest moisturizing effect. This was common to all subjects and showed a similar high moisturizing effect when compared to the two oil mixes (SCJO, SCO) used as a positive control.

[Experimental Example 4] Fatty acid analysis

In order to confirm the index component of the composition according to the present invention, fatty acid analysis was carried out using GC-FID as a methylated test solution using the method described in the Food Code, and the result was analyzed by MFDA Respectively.

Fatty acid analysis was carried out on 37 kinds of fatty acids. As a result, 15 kinds of 37 kinds of fatty acids were detected in the composition of the present invention. Oleic acid corresponding to C18: 1 (n-9) was contained in the oil extracted from plants including olive oil and contained 30.74g / 100g, which was the highest amount of 43.76% of total fatty acids. (N-6, linoleic acid) was 10.4g / 100g (14.80%), followed by 18.65g / 100g (26.55%) of C20: 1 (eicosenoic acid) The results of the analysis are shown in Table 4 below.

fatty acid Fatty acid name Results (g / 100g) Average RSD C4: 0 Butyric acid - ^* - C6: 0 Caproic acid - - C8: 0 Caprylic acid 0.12 0 C10: 0 Capric acid 0.07 20.20 C11: 0 Undecanoic acid - - C12: 0 Lauric acid - - C13: 0 Tridecanoic acid - - C14: 0 Myristic acid 0.01 - C14: 1 (cis-9) Tetradecenoic acid - - C15: 0 Pentadecanoic acid - - C15: 1 (cis-10) Pentadecenoic acid - - C16: 0 Palmitic acid 2.55 3.61 C16: 1
(trans-9) Hexadecenoic acid 0.13 10.88 C17: 0 Margaric acid - - C17: 1 Margaroleic acid 0.03 - C18: 0 Stearic acid One 4.24 C18: 1n-9,
trans Elaidic acid - - C18: 1n-9, cis Oleic acid 30.02 3.39 C18: 2n-6,
trans Linolelaidic acid 0.08 - C18: 2n-6, cis Linoleic acid 10.27 1.79 C20: 0 Arachidic acid 0.2 7.07 C18: 3n-6 γ-Linolenic acid 0.12 - C20: 1 Eicosenoic acid 18.29 2.82 C18: 3n-3 α-Linolenic acid 1.85 8.41 C21: 0 Heneicosanoic acid - - C20: 2 Elcosadienoic acid 0.04 - C22: 0 Behenic acid 0.22 0 C20: 3n-6
(cis-8, 11, 14) Eicosatrienoic acid - - C22: 1n-9 Erucic acid 3.41 3.32 C20: 3n-3
(cis-11,14,17) Eicosatrienoic acid - - C20: 4n-6 Arachidonic acid - - C23: 0 Tricosanoic acid - - C22: 2 Docosadienoic acid - - C24: 0 Lignoceric acid 0.07 0 C20: 5n-3 Eicosapentaenoic acid - - C24: 1 Nervonic acid 0.41 0 C22: 6n-3 Docosahexaenoic acid - -

^* - Not Detection

Meanwhile, in order to analyze the fatty acid of the composition according to the present invention, fatty acid analysis was carried out using 10 kinds of methylated methylated fatty acids as test solutions. As a result, the raw material oil containing the highest amount of fatty acid was sweet almond oil, and its content was 97.11 g / 100 g. Followed by calendula oil at 92.98 g / 100 g.

In the case of jojoba oil as a base oil, 7.32 g / 100 g of C22: 1 (n-9, erucic acid) and 5.23 g / 100 g of C18: 1 (n-9, oleic acid) were identified. Calendula oil, which had 16 kinds of fatty acids, had a large amount of 92.88g / 100g, of which 57.64g / 100g was the majority, and the majority of the fatty acids were C18: 1 (n-9, oleic acid) . Sweet almond oil also showed 64.06g / 100g of C18: 1 (n-9, oleic acid), and more than half of the sweet almond oil showed 66.0% of total fatty acids.

Chamomile oil contained the highest amount of fatty acids among the essential oils. The content of C8: 0 (caprylic acid) and C10: 0 (capric acid) was 29.47 and 18.09 g / 100g, respectively. Similar to chamomile oil, jasmine oil also showed similar tendency to chamomile oil. C8: 0 (caprylic acid) and C10: 0 (capric acid) were found to be 26.15 and 16.02 g / 100g.

In Juniper Berry oil, C4: 0 (butyric acid), which is a low saturated fatty acid in oil constituting the composition of the present invention, was contained in the largest amount of 0.45 g / 100 g, and unsaturated fatty acid C18: 1 (n-9, oleic acid) 0.31 g / 100 g was contained.

Lavender oil was composed of three kinds of fatty acids, and the total amount of fatty acids was 0.51g / 100g. Among them, C10: 0 (Capric acid) accounted for 0.4g / 100g. In the sweet orange oil, 11 kinds of fatty acids were detected in small amount, and the fatty acid having the highest content was C16: 0 (palmitic acid).

Tea tree oil showed only one fatty acid, C8: 0 (caprylic acid), among the 37 fatty acids analyzed.

Only 3 fatty acids were found in the untransformed oil, and 0.14, 0.06 (weight) of C16: 1 (hexadecenoic acid), C17: 1 (margaroleic acid) and C20: 3 (n-3, cis-11,14,17, eicosatrienoic acid) , 0.04 g / 100 g were detected.

In the present experiment, C20: 1 (eicosenoic acid) was 18.65 g / 100 g in the composition of the present invention, accounting for 18.65% of the total fatty acids. However, the calendula oil and sweet almond oil 1.53, and 0.2g / 100g, respectively. The analysis results for the 10 kinds of raw oil are shown in Table 5 below.

fatty acid Fatty acid name Results (g / 100g) Jojoba oil Karen
Dura oil Sweet almond oil Camo
Mile oil Jasmine oil Juniper
Berry
oil Lavender Oil Sweet orange oil Tea tree oil Linseed oil C4: 0 Butyric acid - ^* - - 0.02 - 0.45 0.08 - - - C6: 0 Caproic acid - - - - - - - 0.03 - - C8: 0 Caprylic acid - - - 29.47 26.15 - - 0.2 0.25 - C10: 0 Capric acid - - - 18.09 16.02 - 0.4 0.14 - - C11: 0 Undecanoic acid - - - - - - - - - - C12: 0 Lauric acid - - - 0.03 0.03 - - 0.05 - - C13: 0 Tridecanoic acid - - - - - - 0.03 0.02 - - C14: 0 Myristic acid - 0.03 0.05 0.03 0.02 - - - - - C14: 1
(cis-9) Tetradecenoic acid - - - - - - - - - - C15: 0 Pentadecanoic acid - - - - - - - - - - C15: 1
(cis-10) Pentadecenoic acid - - - - - - - - - - C16: 0 Palmitic acid 0.54 3.83 5.18 0.17 - 0.02 - 0.34 - - C16: 1
(trans-9) Hexadecenoic acid - 0.2 0.52 0.11 0.02 - - 0.06 - 0.14 C17: 0 Margaric acid - 0.06 0.05 0.45 - - - - - - C17: 1 Margaroleic acid - 0.05 0.05 - - 0.05 - - - 0.06 C18: 0 Stearic acid - 1.7 2.38 - 0.04 - - 0.07 - - C18: 1n-9
(trans-9) Elaidic acid - - - - - 0.08 - 0.05 - - C18: 1n-9
(cis-9) Oleic acid 5.23 57.64 64.06 0.24 0.06 0.31 - 0.2 - - C18: 2n-9
(trans-9,
12) Linolelaidic acid - 0.2 0.19 0.41 0.02 - - - - - C18: 2n-9
(cis-9,12) Linoleic acid 0.02 19.25 23.44 0.02 - - - 0.08 - - C20: 0 Arachidic acid - 0.53 0.22 - - - - - - - C18: 3n-5
(cis-5,8,11) γ-Linolenic acid - - - - - - - - - - C20: 1n-11
(cis-11) Eicosenoic acid - 1.53 0.2 - - - - - - - C18: 3n-9
(cis-9, 12, 15) α-Linolenic acid 0.14 7.34 0.09 - - - - - - - C21: 0 Heneicosanoic acid - - - - 0.02 - - - - - C20: 2n-11
(cis-11,14) Eicosadienoic acid - 0.07 - - - - - - - - C22: 0 Behenic acid 0.11 0.28 0.5 0.01 - - - - - - C20: 3n-8
(cis-8, 11, 14) Eicosatrienoic acid - - - - - - - - - - C22: 1n-13
(cis-13) Erucic acid 7.32 - - - - - - - - - C20: 3n-11
(cis-11,
14, 17) Eicosatrienoic acid - - - - - 0.09 - - - 0.04 C20: 4n-5
(cis-5,8,11,14) Arachidonic acid - - - - - - - - - - C23: 0 Tricosanoic acid - - - - - - - - - - C22: 2-10
(cis-10,
13) Docosadienoic acid - - - - - - - - - - C24: 0 Lignoceric acid - 0.13 0.18 - - - - - - - C20: 5n-5
(cis-5,8,11,14,17) Eicosapentaenoic acid - - - - - - - - - - C24: 1 Nervonic acid 0.83 0.14 - - - - - - - - C22: 6n-4
(cis-4,7,10,13,16,19) Docosahexaenoic acid - - - - - - - - - -

^* - Not Detection

[Experimental Example 5] Orientation component analysis

(1) Analysis of aroma components extracted by SDE method

The chromatogram analyzed by GC / MS after extracting the volatile flavor components using the SDE method to analyze the perfume components of the composition of the present invention (blending oil mixed with base oil and essential oil) is shown in FIG. 11, The compounds analyzed qualitatively are shown in Tables 6 and 7 below. A total of 75 volatile flavor components were detected. Among them, Limonene accounted for the highest percentage of about 14%. Linalool, 4-Terpineol and Linalool butyrate, which belong to the monoterpene hydrocarbons, Were 7.87%, 7.45% and 6.89%, respectively. Pheroncide materials of the terpene type represented by? -Pinene were also detected in a high range.

NO RI ²⁾ Compound name MF ³⁾ MW ⁴⁾ Mg / kg One 809 1,1-Diethoxyethane C ₆ H ₁₄ O ₂ 118 175.78 2 925 α-Thujene C ₁₀ H ₁₆ 136 293.18 3 948 α-Fenchene C ₁₀ H ₁₆ 136 283.38 4 973 Camphene C ₁₀ H ₁₆ 136 144.91 5 978 2-β-Pinene C ₁₀ H ₁₆ 136 549.52 6 985 3-Octanone C ₈ H ₁₆ O 128 205.91 7 990 β-Myrcene C ₁₀ H ₁₆ 136 2285.63 8 1004 n-Octanal C ₈ H ₁₆ O 128 84.91 9 1011 2,7-dimethyl-, (E) -3-Octen-5-yne C ₁₀ H ₁₆ 136 6610.45 10 1018 α-Terpinene C ₁₀ H ₁₆ 136 1315.12 11 1025 o-Cymene C ₁₀ H ₁₄ 134 947.83 12 1034 R - (+) - Isolimonene C ₁₀ H ₁₆ 136 16190.55 13 1035 Eucalyptol C ₁₀ H ₁₈ O 154 638.37 14 1036 Z-β-Ocimene C ₁₀ H ₁₆ 136 978.86 15 1047 E-β-Ocimene C ₁₀ H ₁₆ 136 2176.16 I.S . 1056 n- Butylbenzene C ₁₀ H ₁₄ 134 - 16 1060 γ-Terpinene C ₁₀ H ₁₆ 136 3201.12 17 1086 alpha-terpinolene C ₁₀ H ₁₆ 136 1663.24 18 1103 Linalool CH ₁₀ H ₁₈ O 154 9069.41 19 1106 Nonanal C ₉ H ₁₈ O 142 133.25 20 1107 1-Octen-3-yl-acetate C ₁₀ H ₁₈ O ₂ 170 317.16 21 1126 3-Terpineol C ₁₀ H ₁₈ O 154 97.64 22 1149 Camphor C ₁₀ H ₁₆ O 152 102.15 23 1162 Benzyl acetate C ₉ H ₁₀ O ₂ 150 158.42 24 1165 Lavandulol C ₁₀ H ₁₈ O 154 249.82 25 1175 (-) - Borneol C ₁₀ H ₁₈ O 154 342.53 26 1185 4-Terpineol C ₁₀ H ₁₈ O 154 8594.27 27 1189 Cryptone C ₉ H ₁₄ 138 88.26 28 1191 Hexyl Butyrate C ₁₀ H ₂₀ O ₂ 172 124.97 29 1198 α-Terpineol C ₁₀ H ₁₈ O 154 1804.04 30 1206 Decanal C ₁₀ H ₂₀ O 156 116.03 31 1225 Nerol C ₁₀ H ₁₈ O 154 163.37 32 1242 Ascaridole C ₁₀ H ₁₆ O ₂ 168 93.73 33 1252 Linalool butyrate C ₁₄ H ₂₄ O ₂ 224 7941.47 34 1263 (E) -2-Decenal C ₁₀ H ₁₈ O 154 85.39 35 1283 lavandulyl acetate C ₁₂ H ₂₀ O ₂ 196 1084.30 36 1287 Isobornyl acetate C ₁₂ H ₂₀ O ₂ 196 3012.17 37 1296 methyl myrtenate C ₁₁ H ₁₆ O ₂ 180 96.65

No RI ²⁾ Compound name MF ³⁾ MW ⁴⁾ Mg / kg 38 1349 terpinyl acetate C ₁₂ H ₂₀ O ₂ 196 5478.56 39 1357 Neryl acetate C ₁₂ H ₂₀ O ₂ 196 309.42 40 1365 Undec-2-ene C ₁₁ H ₂₀ O 168 86.35 41 1377 Geranyl acetate C ₁₂ H ₂₀ O ₂ 196 554.74 42 1390 β-cubebene C ₁₅ H ₂₄ 204 84.33 43 1391 β-Elemene C ₁₅ H ₂₄ 204 107.33 44 1410 α-Gurjunene C ₁₅ H ₂₄ 204 106.55 45 1421 α-Santalene C ₁₅ H ₂₄ 204 174.91 46 1424 β-Caryophyllene C ₁₅ H ₂₄ 204 1423.38 47 1440 cis-Thujopsene C ₁₅ H ₂₄ 204 1031.53 48 1449 Cadina-3,5-diene C ₁₅ H ₂₄ 204 407.12 49 1453 E-β-Bergamotene C ₁₅ H ₂₄ 204 3129.07 50 1460 α-Humulene C ₁₅ H ₂₄ 204 212.10 51 1466 cis-Muurola-4 (14), 5-diene C ₁₅ H ₂₄ 204 1141.24 52 1478 γ-Muurolene C ₁₅ H ₂₄ 204 239.88 53 1485 Germacrene-D C ₁₅ H ₂₄ 204 405.85 54 1501 α-Muurolene C ₁₅ H ₂₄ 204 505.94 55 1504 α-Farnesene C ₁₅ H ₂₄ 204 347.18 56 1508 α-Cuprenene C ₁₅ H ₂₄ 204 272.95 57 1522 δ-Cadinene C ₁₅ H ₂₄ 204 2691.21 58 1527 1,4-cadinadiene C ₁₅ H ₂₄ 204 1339.94 59 1552 elemol C ₁₅ H ₂₆ O 222 2540.53 60 1573 cis-3-hexenyl benzoate C ₁₃ H ₁₆ O ₂ 204 108.22 61 1581 Spathulenol C ₁₅ H ₂₄ O 220 106.10 62 1587 Caryophyllene oxide C ₁₅ H ₂₄ O 220 132.18 63 1590 Globulol C ₁₅ H ₂₆ O 222 138.48 64 1612 Widdrol C ₁₅ H ₂₆ O 222 98.91 65 1615 alpha-Cedrol C ₁₅ H ₂₆ O 222 328.69 66 1632 Epicubenol C ₁₅ H ₂₆ O 222 299.77 67 1636 γ-Eudesmol C ₁₅ H ₂₆ O 222 355.68 68 1646 epi-α-Cadinol C ₁₅ H ₂₆ O 222 189.23 69 1647 T-Muurolol C ₁₅ H ₂₆ O 222 197.08 70 1660 β-Eudesmol C ₁₅ H ₂₆ O 222 948.91 71 1715 E, E-farnesol C ₂ 0H ₃₄ O 290 83.11 72 1753 alpha -bisabolol oxide C ₁₅ H ₂₆ O ₂ 238 714.51 73 1770 Benzyl benzoate C ₁₄ H ₁₂ O ₂ 212 220.53 74 1905 Rimuene C ₂₀ H ₃₂ 272 97.25 75 1940 Beyerene C ₂₀ H ₃₂ 272 1092.66

²⁾ Retention index, ³⁾ MF = Molecular formula, ⁴⁾ MW = Molecular weight

(2) Analysis of aroma components extracted by SPME method

In order to analyze the fragrance component of the composition of the present invention (blending oil mixed with base oil and essential oil), volatile fragrance components were extracted by using SPME method having a shorter pretreatment time than SDE method, and analyzed by GC / MS The chromatograms are shown in FIG. 12, and the compounds analyzed qualitatively are shown in Tables 8 to 10 below.

As a result of analysis, total 78 kinds of fragrance components were identified. Limonene among the total volatile fragrance components occupied the largest proportion of 8%, and Linalool, 4-Terpineol, and Linalool butyrate and α-pinene were also detected in the same high range.

As described above, it was confirmed that most of the compounds were consistent with the volatile aroma component analysis results using the SDE method. However, the SDE pretreatment method requires a long time, The existence of the variables led us to conclude that the SPME method is a more appropriate and desirable method than the SDE method.

No RI ²⁾ Compound name MF ³⁾ MW ⁴⁾ Mg / kg One 905 α-Thujene C ₁₀ H ₁₆ 136 233.78 2 930 α-Fenchene C ₁₀ H ₁₆ 136 168.86 3 932 Camphene C ₁₀ H ₁₆ 136 90.84 4 963 beta-Pinene C ₁₀ H ₁₆ 136 271.40 5 967 1-Octen-3-ol C ₈ H ₁₆ O 128 28.52 6 973 3-Octanone C ₈ H ₁₆ O 128 153.08 7 977 Myrcene C ₁₀ H ₁₆ 136 1559.64 8 986 ethyl-hexanol C ₈ H ₁₈ O 130 29.73 9 993 n-Octanal C ₈ H ₁₆ O 128 84.91 10 996 alpha-Phellandrene C ₁₀ H ₁₆ 136 67.96 11 999 3-carene C ₁₀ H ₁₆ 136 4401.43 12 1003 1-Hexyl acetate C ₈ H ₁₆ O ₂ 144 77.78 13 1007 α-Terpinene C ₁₀ H ₁₆ 136 713.72 14 1016 o-Cymene C ₁₀ H ₁₄ 134 830.54 15 1022 dl-Limonene C ₁₀ H ₁₆ 136 11621.27 16 1024 Eucalyptol C ₁₀ H ₁₈ O 154 395.80 17 1028 o-Cymene C ₁₀ H ₁₆ 136 536.59 18 1039 Z-β-Ocimene C ₁₀ H ₁₆ 136 320.78 I.S . 1048 n- Butylbenzene C ₁₀ H ₁₄ 134 - 19 1052 γ-Terpinene C ₁₀ H ₁₆ 136 1543.99 20 1064 cis-Sabinene hydrate C ₁₀ H ₁₈ O 154 48.12 21 1076 Isoterpinolene C ₁₀ H ₁₆ 136 42.17 22 1080 α-TERPINOLENE C ₁₀ H ₁₆ 136 760.67 23 1096 Linalool C ₁₀ H ₁₈ O 154 3473.65 24 1102 1-Octen-3-yl-acetate C ₁₀ H ₁₈ O ₂ 170 133.20 25 1108 Phenethyl alcohol C ₈ H ₁₀ O 122 29.89 26 1121 3-Terpineol C ₁₀ H ₁₈ O 154 27.89 27 1124 E, Z-alloocimene C ₁₀ H ₁₆ 136 55.08 28 1145 Camphor C ₁₀ H ₁₆ O 152 47.33

No RI ²⁾ Compound name MF ³⁾ MW ⁴⁾ Mg / kg 29 1159 Benzyl acetate C9H ₁₀ O ₂ 150 143.02 30 1161 Lavandulol C ₁₀ H ₁₈ O 154 110.49 31 1171 (-) - Borneol C ₁₀ H ₁₈ O 154 109.02 32 1180 4-Terpineol C ₁₀ H ₁₈ O 154 3408.14 33 1185 Cyptone C ₉ H ₁₄ O 138 82.14 34 1188 hexyl-Butyrate C ₁₀ H ₂₀ O ₂ 172 47.42 35 1193 α-Terpineol C ₁₀ H ₁₈ O 154 495.64 36 1204 Decanal C ₁₀ H ₂₀ O 156 116.03 37 1222 Nerol C ₁₀ H ₁₈ O 154 163.37 38 1242 Ascaridole C ₁₀ H ₁₂ O 148 93.73 39 1248 Linalool butyrate C ₁₂ H ₂₀ O ₂ 196 3770.33 40 1281 lavandulyl acetate C ₁₂ H ₂₀ O ₂ 196 401.98 41 1284 Isobornyl acetate C ₁₂ H ₂₀ O ₂ 196 1131.50 42 1290 Indolizine C ₈ H ₇ N 117 33.71 43 1295 methyl myrtenate C ₁₁ H ₁₆ O ₂ 180 27.95 44 1340 Methyl anthranilate C ₈ HNO ₂ 151 95.53 45 1347 alpha-terpinyl acetate C ₁₂ H ₂₀ O ₂ 196 2180.65 46 1356 Neryl acetate C ₁₂ H ₂₀ O ₂ 196 97.76 47 1376 Geranyl acetate C ₁₂ H ₂₀ O ₂ 196 164.35 48 1390 Beta ELEMENE C ₁₅ H ₂₄ 204 55.66 49 1409 Valerena-4,7-diene C ₁₅ H ₂₄ 204 31.24 50 1420 α-Santalene C ₁₅ H ₂₄ 204 47.95 51 1422 Caryophyllene C ₁₅ H ₂₄ 204 340.73 52 1439 cis-Thujopsene C ₁₅ H ₂₄ 204 263.29 53 1448 Cadina-3,5-diene C ₁₅ H ₂₄ 204 91.39 54 1452 E-β-Bergamotene C ₁₅ H ₂₄ 204 746.45 55 1459 α-Humulene C ₁₅ H ₂₄ 204 49.93 56 1465 cis-Muurola-4 (14), 5-diene C ₁₅ H ₂₄ 204 269.09 57 1474 Cadina-1 (6), 4-diene C ₁₅ H ₂₄ 204 314.31

No RI ²⁾ Compound name MF ³⁾ MW ⁴⁾ Mg / kg 58 1477 γ-Muurolene C ₁₅ H ₂₄ 204 57.36 59 1484 Germacrene D C ₁₅ H ₂₄ 204 54.58 60 1500 α-Muurolene C ₁₅ H ₂₄ 204 112.77 61 1503 (E, E) -? - Farnesene C ₁₅ H ₂₄ 204 51.66 62 1506 α-Cuprenene C ₁₅ H ₂₄ 204 54.87 63 1515 gamma.-Cadinene C ₁₅ H ₂₄ 204 97.71 64 1520 delta.-Cadinene C ₁₅ H ₂₄ 204 600.67 65 1524 cis-calamenene C ₁₅ H ₂₂ 202 253.19 66 1544 α-Calacorene C ₁₅ H ₂₀ 200 21.97 67 1550 elemol C ₁₅ H ₂₆ O 222 443.83 68 1572 (3Z) -hexenyl-benzoate C ₁₃ H ₁₆ O ₂ 204 30.75 69 1586 Caryophyllene oxide C ₁₅ H ₂₄ O 220 23.59 70 1614 alpha-Cedrol C ₁₈ H ₃₂ O 264 53.20 71 1631 Epicubenol C ₁₅ H ₂₆ O 222 49.59 72 1635 gamma-Eudesmol C ₁₅ H ₂₆ O 222 59.18 73 1645 epi-α-Cadinol C ₁₅ H ₂₆ O 222 26.51 74 1646 T-Muurolol C ₁₅ H ₂₆ O 222 32.38 75 1659 β-Eudesmol C ₁₅ H ₂₆ O 222 168.19 76 1752 alpha -bisabolol oxide C ₁₅ H ₂₆ O ₂ 238 81.24 77 1939 Beyerene C ₂₀ H ₃₂ 272 95.76

²⁾ Retention index, ³⁾ MF = Molecular formula, ⁴⁾ MW = Molecular weight

(3) Comparison of volatile flavor components using SDE and SPME pretreatment

As a result of analyzing the fragrance components of the composition of the present invention (blending oil mixed with base oil and essential oil) using SDE and SPME pretreatment method, it was found that many of the same compounds were detected in both cases, but only SDE or SPME pretreatment method The compounds to be analyzed were identified.

Chromatograms showed that the same compounds had a higher area peak when using the SDE method, which was expected to result in higher concentrations due to the extraction and concentration of the SDE method. In the case of the compounds specifically analyzed in the SDE or SPME pretreatment method, it was judged that the degeneration of the fragrance component was caused by the difference of the SDE method including the heat treatment unlike the SPME method using the sample as it is. Therefore, in the following experiment, the SPME method is used as an index component for the composition of the present invention in which each individual raw material is mixed. The following Tables 11 to 13 are tables comparing volatile aroma component analysis results using the SDE and SPME pretreatment methods, and FIG. 13 shows the volatile flavor component analysis chromatogram results using the SDE and SPME pretreatment methods.

No RI ²⁾ Compound name SDE SDE Area (%) SPME SPME
Area (%) One 809 1,1-Diethoxyethane O 0.17 2 905 α-Thujene O 0.28 0 0.52 3 914 α-Pinene O 3.54 4 930 α-Fenchene O 0.27 0 0.38 5 932 Camphene O 3.27 O 0.20 6 963 β-Pinene O 0.52 O 0.61 7 967 1-Octen-3-ol O 0.06 8 973 3-Octanone O 0.19 O 0.34 9 977 Myrcene O 3.48 10 986 Ethyl-Hexanol O 0.07 11 990 β-Myrcene O 2.16 12 993 n-Octanal O 0.08 O 0.08 13 996 α-Phellandrene O 0.15 14 999 3-Carene O 9.83 15 1003 1-Hexyl acetate O 0.17 16 1007 α-Terpinene O 1.24 O 1.59 17 1011 (E) -2,7-Dimethyl-3-Octen-5-yne O 6.24 18 1016 o-Cymene O 0.90 O 1.85 19 1022 Limonene O 15.29 O 25.95 20 1024 Eucalyptol O 0.6 O 0.88 21 1039 (Z) -β-Ocimene O 0.62 O 0.72 I.S. 1048 n-Butylbenzene O O 22 1052 γ-Terpinene O 3.02 O 3.45 23 1064 (Z) -Sabineene hydrate O 0.11 24 1076 Isoterpinolene O 0.09 25 1080 α-TERPINOLENE O 1.57 O 1.70 26 1096 Linalool O 8.56 O 7.76 27 1102 1-Octen-3-yl-acetate O 0.30 O 0.30 28 1106 Nonanal O 0.13

No RI ²⁾ Compound name SDE SDE Area (%) SPME SPME
Area (%) 29 1108 Phenethyl alcohol O 0.07 30 1121 3-Terpineol O 0.09 O 0.06 31 1124 (E, Z) -Alloocimene O 0.12 32 1145 Camphor O 0.10 O 0.11 33 1159 Benzyl acetate O 0.15 O 0.32 34 1161 Lavandulol O 0.24 O 0.25 35 1171 (-) - Borneol O 0.32 O 0.24 36 1180 4-Terpineol O 8.12 O 7.61 37 1185 Cryptone O 0.08 O 0.18 38 1188 Hexyl-Butyrate O 0.12 O 0.11 39 1193 α-Terpineol O 1.70 O 1.11 40 1204 Decanal O 0.11 O 0.09 41 1222 Nerol O 0.15 O 0.10 42 1242 Ascaridole O O 0.09 43 1252 Linalool butyrate O 7.50 O 8.42 44 1263 (E) -2-Decenal O 0.08 45 1281 Lavandulyl acetate O 1.02 O 0.90 46 1287 Isobornyl acetate O 2.84 O 2.53 47 1290 Indolizine O 0.08 48 1295 Methyl myrtenate O 0.09 O 0.06 49 1340 Methyl anthranilate O 0.21 50 1347 alpha-terpinyl acetate O 5.17 O 4.87 51 1356 Neryl acetate O 0.29 O 0.22 52 1365 Undec-2-ene O 0.08 53 1376 Geranyl acetate O 0.52 O 0.37 54 1390 β-Elemene O 0.1 O 0.12 55 1390 β-Cubebene O 0.08 56 1409 Valerena-4,7-diene O 0.07 57 1410 α-Gurjunene O 0.10

No RI ²⁾ Compound name SDE SDE Area (%) SPME SPME
Area (%) 58 1420 α-Santalene O 0.17 O 0.11 59 1422 Caryophyllene O 1.34 O 0.76 60 1439 (Z) -Thujopsene O 0.97 O 0.59 61 1448 Cadina-3,5-diene O 0.38 O 0.2 62 1453 (E) -β-Bergamotene O 2.95 O 1.67 63 1459 α-Humulene O 0.20 O 0.11 64 1465 (Z) -Muurola-4,5-diene O 1.08 O 0.60 65 1474 Cadina-1,4-diene O 0.70 66 1477 γ-Muurolene O 0.23 O 0.13 67 1485 Germacrene-D O 0.38 O 0.12 68 1500 α-Muurolene O 0.48 O 0.25 69 1503 (E, E) -? - Farnesene O 0.12 70 1504 α-Farnesene O 0.33 71 1506 α-Cuprenene O 0.12 72 1515 γ-Cadinene 0 0.22 73 1522 δ-Cadinene O 2.54 O 1.34 74 1524 (Z) -Calamene O 0.57 75 1527 1.4-Cadinadiene O 1.27 76 1544 α-Calacorene O 0.05 77 1550 Elemol O 2.40 O 0.99 78 1572 (Z) -Hexenyl-Benzoate O 0.10 O 0.07 79 1581 Spathulenol O 0.10 80 1586 Caryophyllene oxide O 0.12 O 0.05 81 1590 Globulol O 0.13 82 1612 Widdrol O 0.09 83 1615 alpha-Cedrol O 0.31 O 0.12 84 1631 Epicubenol O 0.28 O 0.11 85 1635 γ-Eudesmol O 0.34 O 0.13 86 1645 Epi-α-Cadinol O 0.18 O 0.06 87 1646 T-Muurolol O 0.19 O 0.07 88 1659 β-Eudesmol O 0.90 O 0.38 89 1715 Farnesol O 0.08 90 1752 α-Bisabolol oxide O 0.67 O 0.18 91 1770 Benzyl benzoate O 0.21 92 1905 Rimuene O 0.09 93 1939 Beyerene O 1.03 O 0.21

²⁾ Retention index

(4) Flavor component analysis of the main raw material of the composition (blending oil) of the present invention

In order to set the index component and the reference value range for the composition of the present invention, the index component for each volatile essential oil component of 10 different substances was set by GC-MS.

In order to analyze the volatile fragrance components, the fragrance components were extracted using SDE and SPME pretreatment method for the blending oil, and analyzed by GC-MS. As a result, it was determined that the SPME method was more suitable, Were pretreated with SPME method.

The volatile essential oil component analysis results and the blending oil analysis results for each individual raw material were compared with each other to select representative aromatic components and specific components contained in the individual raw materials. Finally, by comparing with the components of other raw materials, The surface components were selected.

1) Analysis of Volatile Flavor Components of Jojoba Oil and Survey of Surface Composition

The volatile fragrance components of jojoba oil, which has the highest content as the raw material of blending oil, were found to contain a small amount of fragrance components, and the most likely components were Methyl anthranilate (peak 1), (Z) -Hexenyl-Benzoate 2). However, Jojoba oil has a low volatile fragrance content and therefore it is necessary to further analyze fatty acid or wax for more accurate selection.

The volatile flavor component analysis of jojoba oil is shown in Table 14 below.

No RI ²⁾ Compound name MF ³⁾ MW ⁴⁾ G / g One 925 α-Thujene 136 C ₁₀ H ₁₆ 0.104 2 932 α-Pinene 136 C ₁₀ H ₁₆ 0.394 3 972 Sabinene 136 C ₁₀ H ₁₆ 0.659 4 989 Myrcene 136 C ₁₀ H ₁₆ 0.877 5 1008 3-Carene 136 C ₁₀ H ₁₆ 1.016 6 1016 alpha-terpinolene 136 C ₁₀ H ₁₆ 0.112 7 1024 ρ-Cymene 134 C ₁₀ H ₁₄ 0.492 8 1029 Limonene 136 C ₁₀ H ₁₆ 12.164 I.S 1056 Butylbenzene 134 C ₁₀ H ₁₄ - 9 1058 γ-Terpinene 136 C ₁₀ H ₁₆ 0.543 10 1085 α-Terpinene 136 C ₁₀ H ₁₆ 0.144 11 1099 Linalool 154 C ₁₀ H ₁₈ O 0.092 12 1105 Nonanal 142 C ₉ H ₁₈ O 0.386 13 1182 Terpinen-4-ol 154 C ₁₀ H ₁₈ O 0.271 14 1200 Dodecane 170 C ₁₂ H ₂₆ 0.460 15 1285 Isobornyl acetate 196 C ₁₂ H ₂₀ O ₂ 0.062 16 1600 Hexadecane 226 C ₁₆ H ₃₄ 0.065 17 1771 Benzyl benzoate 212 C ₁₄ H ₁₂ O ₂ 0.056

²⁾ Retention index, ³⁾ MF = Molecular formula, ⁴⁾ MW = Molecular weight

2) Analysis of volatile flavor components of calendula oil

As a result of analysis of volatile fragrance components of calendula oil using SPME method, a small amount of aroma component was detected like jojoba oil, and the most likely compound was detected at 47.99 minutes by isobornyl acetate. However, it is considered that fatty acid analysis is necessary for more accurate analysis because the concentration of the fragrance component is relatively small.

The volatile flavor component analysis of calendula oil is shown in Table 15 below.

No RI ²⁾ Compound name MF ³⁾ MW ⁴⁾ G / g One 933 α-Pinene 136 C ₁₀ H ₁₆ 0.295 2 972 Sabinene 136 C ₁₀ H ₁₆ 0.768 3 989 Myrcene 136 C ₁₀ H ₁₆ 0.771 4 1008 δ-Carene 136 C ₁₀ H ₁₆ 0.576 5 1025 ρ-Cymene 134 C ₁₀ H ₁₄ 0.659 6 1029 Limonene 136 C ₁₀ H ₁₆ 7.805 I.S 1056 Butylbenzene 134 C ₁₀ H ₁₄ - 7 1059 γ-Terpinene 136 C ₁₀ H ₁₆ 0.639 8 1085 Terpinolene 136 C ₁₀ H ₁₆ 0.096 9 1182 4-Terpineol 154 C ₁₀ H ₁₈ O 0.334 10 1216 2,4-Dimethylbenzaldehyde 134 C ₉ H ₁₀ O 0.322 11 1274 2,6,8-Trimethyldecane 142 C ₁₀ H ₂₂ 0.211 12 1285 Isobornyl acetate 194 C ₁₃ H ₂₂ O 0.091 13 1342 Methyl anthranilate 151 C ₈ H ₉ NO ₂ 0.103 14 1400 pentadecane 212 C ₁₅ H ₃₂ 0.248 15 1474 1-Undecanol 172 C ₁₁ H ₂₄ O 0.544

²⁾ Retention index, ³⁾ MF = Molecular formula, ⁴⁾ MW = Molecular weight

3) Analysis of volatile flavor components of sweet almond oil

Analysis of the volatile fragrance components of sweet almond oil revealed that a small amount of fragrance components were analyzed as well as the two oils (jojoba oil, calendula oil). Among them, 3-Carene was selected as a candidate compound for the index component of sweet almond oil. However, fragrance components were detected in a small amount and a little concentration as in the case of jojoba and calendula oil.

The volatile fragrance component analysis of sweet almond oil is shown in Table 16 below.

No RI ²⁾ Compound name MF ³⁾ MW ⁴⁾ G / g One 933 α-Pinene 136 C ₁₀ H ₁₆ 2.059 2 972 Sabinene 136 C ₁₀ H ₁₆ 4.841 3 983 β-Ionone epoxide 208 C ₁₃ H ₂₀ O ₂ 0.448 4 989 Myrcene 136 C ₁₀ H ₁₆ 4.201 5 1008 3-Carene 136 C ₁₀ H ₁₆ 3.781 6 1025 ρ-cymene 134 C ₁₀ H ₁₄ 3.188 7 1029 Limonene 136 C ₁₀ H ₁₆ 50.943 I.S 1056 1-butylbenzene 134 C ₁₀ H ₁₄ 19.370 8 1059 γ-Terpinene 136 C ₁₀ H ₁₆ 3.492 9 1085 Betahistine 136 C ₈ H ₁₂ N ₂ 0.655 10 1182 4-Terpineol 154 C ₁₀ H ₁₈ O 1.290 11 1217 2,4-Dimethyl benzaldehyde 134 C ₉ H ₁₀ O 1.966 12 1248 1,4-Di-tert-butylbenzene 190 C ₁₄ H ₂₂ 0.885 13 1400 2,3,6-Trimethylheptane 142 C ₁₀ H ₂₂ 1.875 14 1474 1-Octadecanol 270 C ₁₈ H ₃₈ O 1.300 15 1626 2-2-aminoethylamino-ethylamine 103 C ₄ H ₁₃ N ₃ 0.432 16 1699 Cyclobutanol 72 C ₄ H ₈ O 0.234 17 1771 Benzyl benzoate 212 C ₁₄ H ₁₂ O ₂ 0.609

²⁾ Retention index, ³⁾ MF = Molecular formula, ⁴⁾ MW = Molecular weight

4) Analysis of Volatile Flavor Components of Chamomile Oil

As a result of analyzing volatile fragrance components of chamomile oil using SPME, many fragrant components were detected. Among the various components, compounds rich in the possibility of being an indicator component of the chamomile oil were peaks 1 and 2, respectively, and trans-β-farnesene and Bisabolol oxide A, respectively.

The results of analysis of volatile flavor components of chamomile oil are shown in Table 17 below.

No RI ²⁾ Compound name MF ³⁾ MW ⁴⁾ Mg / g One 1047 Sabinene 136 C ₁₀ H ₁₆ 12.45 I.S 1056 Butylbenzene 134 C ₁₀ H ₁₄ - 2 1058 Artemisia ketone 152 C ₁₀ H ₁₆ O 23.41 3 1342 Methyl anthranilate 151 C ₈ H ₉ NO ₂ 17.29 4 1423 β-Caryophyllene 204 C ₁₅ H ₂₄ 20.05 5 1453 trans-β-farnesene 204 C ₁₅ H ₂₄ 1343.78 6 1485 germacrene D 204 C ₁₅ H ₂₄ 34.58 7 1490 cis-β-farnesene 204 C ₁₅ H ₂₄ 15.224 8 1499 Bicyclogermacrene 204 C ₁₅ H ₂₄ 49.36 9 1504 (E, E) -α-farnesene 204 C ₁₅ H ₂₄ 188.55 10 1509 β-Bisabolene 204 C ₁₅ H ₂₄ 6.15 11 1521 β-cadinene 204 C ₁₅ H ₂₄ 8.46 12 1581 Spathulenol 220 C ₁₅ H ₂₄ O 27.50 13 1600 3,3-Dimethylundecane 184 C ₁₃ H ₂₈ 16.60 14 1658 α-Bisabolol oxide B 238 C ₁₅ H ₂₆ O ₂ 50.39 15 1700 Nor-pristane 254 C ₁₈ H ₃₈ 17.28 16 1703 5-propylnonane 170 C ₁₂ H ₂₆ 8.82 17 1753 Bisabolol oxide A 238 C ₁₅ H ₂₆ O ₂ 126.26 18 1771 Benzyl benzoate 212 C ₁₄ H ₁₂ O ₂ 24.40 19 1806 Phytane 282 C ₂₀ H ₄₂ 25.89 20 1900 Farnesane 212 C ₁₅ H ₃₂ 20.52

²⁾ Retention index, ³⁾ MF = Molecular formula, ⁴⁾ MW = Molecular weight

5) Analysis of volatile flavor components of jasmine oil

As a result of analyzing the fragrance component of jasmine oil using SPME method, various compounds were analyzed and two components that were shrunk to the index component of jasmine oil as compared with blending oil were selected. Peaks 1, 2, and 3 detected at 28.74, 34, and 38 minutes of R.T for both jasmine oil and blending oil were identified as benzyl alcohol, linalool, and benzyl acetate. However, jasmone, which is a typical flavor component of jasmine oil, was not detected and further experiments were required to ensure its reliability.

The volatile flavor components of jasmine oil are shown in Table 18 below.

No RI ²⁾ Compound name MF ³⁾ MW ⁴⁾ Mg / kg One 933 5,6,7,8-tetrahydroindolizin-7-ol 137 C ₈ H ₁₁ NO 2.54 2 1005 Sabinene hydrate 154 C ₁₀ H ₁₈ O 10.30 3 1005 Leaf acetate 142 C ₈ H ₁₄ O ₂ 65.15 4 1029 Limonene 136 C ₁₀ H ₁₆ 1.81 5 1031 α-Phellandrene 136 C ₁₀ H ₁₆ 2.12 6 1034 Benzyl alcohol 108 C ₇ H ₈ O 1233.05 I.S 1056 Butylbenzene 134 C ₁₀ H ₁₄ - 7 1072 ρ-Cresol 108 C ₇ H ₈ O 152.69 8 1087 trans-Linalool oxide 170 C ₁₀ H ₁₈ O ₂ 8.99 9 1094 Methyl benzoate 136 C ₈ H ₈ O ₂ 18.16 10 1099 Linalool 154 C ₁₀ H ₁₈ O 303.00 11 1112 2-Phenylethanol 122 C ₈ H ₁₀ O 255.11 12 1150 2-Methyl-6-methylene-7-octen-4-ol 154 C ₁₀ H ₁₈ O 3.28 13 1163 Benzyl acetate 150 C ₉ H ₁₀ O ₂ 1179.79 14 1192 Methyl salicylate 152 C ₈ H ₈ O ₃ 11.35 15 1196 α-Terpineol 154 C ₁₀ H ₁₈ O 134.32 16 1200 Terpinyl propionate 210 C ₁₃ H ₂₂ O ₂ 21.37 17 1254 Phenethyl acetate 164 C ₁₀ H ₁₂ O ₂ 92.76 18 1292 1H-indole 117 C ₈ H ₇ N 102.17 19 1342 Methyl anthranilate 151 C ₈ H ₉ NO ₂ 481.48 20 1408 Dimethyl anthranilate 165 C ₉ H ₁₁ NO ₂ 27.01 21 1452 cis-β-Farnesene 204 C ₁₅ H ₂₄ 12.67 22 1482 α-curcumene 202 C ₁₅ H ₂₂ 12.74 23 1496 α-Funebrene 204 C ₁₅ H ₂₄ 13.31 24 1503 Synephrine 167 C ₉ H ₁₃ NO ₂ 2.47 25 1509 TRANS-α-BERGAMOTENE 248 C ₁₅ H ₂₀ O ₃ 5.62 26 1530 Neryl formate 182 C ₁₁ H ₁₈ O ₂ 13.42 27 1561 Nerolidol 222 C ₁₅ H ₂₆ O 20.65 28 1573 cis-3-hexenyl benzoate 204 C ₁₃ H ₁₆ 0 ₂ 249.49 29 1699 2,5-dimethylhexen-3-one 128 C ₈ H ₁₆ O 3.91 30 1770 Benzyl benzoate 212 C ₁₄ H ₁₂ O ₂ 328.79 31 1858 Phenethyl benzoate 226 C ₁₅ H ₁₄ O ₂ 10.96 32 1899 Lauryl ether 354 C ₂₄ H ₅₀ O 4.37

²⁾ Retention index, ³⁾ MF = Molecular formula, ⁴⁾ MW = Molecular weight

6) Analysis of Volatile Flavor Components of Juniper Berry Oil and Survey of Surface Composition

As a result of analyzing the fragrance components of Juniper berries oil using SPME method, various volatile organic compounds were detected. Among them, three compounds which are likely to be an indicator component of Juniper berry oil were selected. The components were identified as α-Pinene, Sabinene, and 1-Terpineol, respectively.

Table 19 and Table 20 show the analysis results of the volatile flavor components of Juniper Berry oil.

No RI ²⁾ Compound name MF ³⁾ MW ⁴⁾ Mg / kg One 926 α-Thujene 136 C ₁₀ H ₁₆ 9.27 2 933 α-Pinene 136 C ₁₀ H ₁₆ 819.80 3 948 α-Fenchene 136 C ₁₀ H ₁₆ 105.75 4 973 Sabinene 136 C ₁₀ H ₁₆ 65.24 5 977 β-Pinene 136 C ₁₀ H ₁₆ 21.69 6 989 Myrcene 136 C ₁₀ H ₁₆ 189.07 7 1008 3-Carene 136 C ₁₀ H ₁₆ 2425.39 8 1025 O-Cymene 134 C ₁₀ H ₁₄ 49.58 9 1030 Limonene 136 C ₁₀ H ₁₆ 699.21 I.S 1056 1-Butylbenzene 134 C ₁₀ H ₁₄ - 10 1059 γ-Terpinene 136 C ₁₀ H ₁₆ 17.16 11 1086 alpha-terpinolene 136 C ₁₀ H ₁₆ 129.97 12 1090 2-p-tolylpropene 132 C ₁₀ H ₁₂ 20.77 13 1144 α-Thujone 152 C ₁₀ H ₁₆ O 17.51 14 1164 alpha -Phellandlen-8-ol 152 C ₁₀ H ₁₆ O 31.31 15 1182 Terpinen-4-ol 154 C ₁₀ H ₁₈ O 90.80 16 1188 p-cymen-8-ol 150 C ₁₀ H ₁₄ O 55.70 17 1189 1-Terpineol 154 C ₁₀ H ₁₈ O 22.67 18 1242 Hexyl isovalerate 186 C ₁₁ H ₂₂ O ₂ 23.85 19 1280 Limonene dioxide 168 C ₁₀ H ₁₆ O ₂ 13.41 20 1286 Bornyl acetate 196 C ₁₂ H ₂₀ O ₂ 33.34 21 1309 Chrysanthenone 150 C ₁₀ H ₁₄ O 36.97 22 1349 α-Cubebene 204 C ₁₅ H ₂₄ 157.55 23 1379 alpha-Copaene 204 C ₁₅ H ₂₄ 42.46 24 1390 β-Cubebene 204 C ₁₅ H ₂₄ 77.89 25 1420 β-Cedrene 204 C ₁₅ H ₂₄ 40.04 26 1424 cis-Caryophyllene 204 C ₁₅ H ₂₄ 72.47 27 1431 γ-Elemene 204 C ₁₅ H ₂₄ 12.10 28 1440 cis-Thujopsene 204 C ₁₅ H ₂₄ 10.01

No RI ²⁾ Compound name MF ³⁾ MW ⁴⁾ Mg / kg 29 1460 alpha-caryophyllene 204 C ₁₅ H ₂₄ 85.99 30 1478 Cadina-1 (6), 4-diene 204 C ₁₅ H ₂₄ 653.11 31 1478 γ-Muurolene 204 C ₁₅ H ₂₄ 109.04 32 1485 Germacrene D 204 C ₁₅ H ₂₄ 8.13 33 1496 cis-4,5-Muuroladiene 204 C ₁₅ H ₂₄ 1028.12 34 1499 4-epi-Cubebol 222 C ₁₅ H ₂₆ O 57.51 35 1501 `α-Muurolene 204 C ₁₅ H ₂₄ 308.30 36 1516 γ-Cadinene 204 C ₁₅ H ₂₄ 246.42 37 1521 δ-Cadinene 204 C ₁₅ H ₂₄ 1986.30 38 1525 cis-Calamenene 202 C ₁₅ H ₂₂ 1676.12 39 1536 trans-Cadina-1,4-diene 204 C ₁₅ H ₂₄ 175.48 40 1540 α-Cadinene 204 C ₁₅ H ₂₄ 57.40 41 1545 α-Calacorene 200 C ₁₅ H ₂ O 59.83 42 1551 Elemol 222 C ₁₅ H ₂₆ O 83.06 43 1564 α-Bulnesene 204 C ₁₅ H ₂₄ 15.42 44 1566 β-Calacorene 200 C ₁₅ H ₂ O 28.55 45 1587 Caryophyllene oxide 220 C ₁₅ H ₂₄ O 15.54 46 1590 Gleenol 222 C ₁₅ H ₂₆ O 14.15 47 1602 Allocedrol 222 C ₁₅ H ₂₆ O 20.63 48 1608 β-Oplopenone 220 C ₁₅ H ₂₄ O 17.80 49 1615 Cedrol 222 C ₁₅ H ₂₆ O 256.81 50 1632 1-epi-Cubenol 222 C ₁₅ H ₂₆ O 350.55 51 1636 γ-Eudesmol 222 C ₁₅ H ₂₆ O 14.24 52 1648 T-Muurolol 222 C ₁₅ H ₂₆ O 200.64 53 1650 Torreyol 222 C ₁₅ H ₂₆ O 31.80 54 1660 α-Cadinol 222 C ₁₅ H ₂₆ O 144.42 55 1684 β-Copaene-4α-ol 220 C ₁₅ H ₂₄ O 28.44

²⁾ Retention index, ³⁾ MF = Molecular formula, ⁴⁾ MW = Molecular weight

7) Analysis of Volatile Flavor Components of Lavender Oil and Survey of Surface Composition

The volatile flavor components of lavender oil and the fragrance components of blending oils were analyzed by GC-MS using SPME. Various fragrance components were detected and the compound 2 Species were identified and Peak 1 and Peak 2 were identified as Lavanduly acetate and Geranyl acetate, respectively.

The following Table 21 and Table 22 show the volatile flavor component analysis results of lavender oil.

No RI ²⁾ Compound name MF ³⁾ MW ⁴⁾ Mg / kg One 977 β-Pinene 136 C ₁₀ H ₁₆ 59.12 2 980 [delta] -I-Octen-3-ol 128 C ₈ H ₁₆ O 27.22 3 985 Octen-3-one 128 C ₈ H ₁₆ O 65.20 4 989 β-Myrcene 136 C ₁₀ H ₁₆ 161.05 5 1013 Hexyl acetate 144 C ₈ H ₁₆ O ₂ 68.33 6 1029 Limonene 136 C ₁₀ H ₁₆ 65.45 7 1032 Eucalyptol 154 C ₁₀ H ₁₈ O 177.21 8 1036 α-Pinene 136 C ₁₀ H ₁₆ 63.77 9 1047 3-Carene 136 C ₁₀ H ₁₆ 81.20 I.S 1056 1-Butylbenzene 134 C ₁₀ H ₁₄ - 10 1071 cis-Linalool oxide 170 C ₁₀ H ₁₈ O ₂ 4.89 11 1100 Linalool 154 C ₁₀ H ₁₈ O 4100.11 12 1119 Octan-3-yl acetate 172 C ₁₀ H ₂₀ O ₂ 21.86 13 1149 Camphor 152 C ₁₀ H ₁₆ O 188.85 14 1173 1S-borneol 154 C ₁₀ H ₁₈ O 133.30 15 1182 Terpinen-4-ol 154 C ₁₀ H ₁₈ O 205.81 16 1188 3-hexenyl isobutanoate 170 C ₁₀ H ₁₈ O ₂ 12.22 17 1191 Hexyl butyrate 172 C ₁₀ H ₂₀ O ₂ 11.70 18 1196 α-Terpineol 154 C ₁₀ H ₁₈ O 344.89 19 1229 β-Terpineol 196 C ₁₂ H ₂₀ O ₂ 56.31 20 1242 Hexyl isovalerate 186 C ₁₁ H ₂₂ O ₂ 5.98 21 1250 Linalyl acetate 196 C ₁₂ H ₂₀ O ₂ 10756.75

No RI ²⁾ Compound name MF ³⁾ MW ⁴⁾ Mg / kg 22 1268 Dihydro linalool 156 C ₁₀ H ₂₀ O 146.21 23 1282 Lavandulyl acetate 196 C ₁₂ H ₂₀ O ₂ 96.92 24 1329 Hexyl tiglate 184 C ₁₁ H ₂₀ O ₂ 8.68 25 1342 Methyl anthranilate 151 C ₈ H ₉ O ₂ 212.00 26 1358 Neryl acetate 196 C ₁₂ H ₂₀ O ₂ 86.20 27 1377 Geranyl acetate 196 C ₁₂ H ₂₀ O ₂ 709.11 28 1408 Caryophyllene 204 C ₁₅ H ₂₄ 36.28 29 1424 β-Caryophyllene 204 C ₁₅ H ₂₄ 3252.52 30 1453 beta-trans-Bergamotene 204 C ₁₅ H ₂₄ 66.72 31 1457 Neryl butyrate 224 C ₁₄ H ₂₄ O ₂ 5.89 32 1460 alpha-caryophyllene 204 C ₁₅ H ₂₄ 86.60 33 1485 Germacrene D 204 C ₁₅ H ₂₄ 28.36 34 1500 Lavandulyl isovalerate 238 C ₁₅ H ₂₆ O ₂ 28.69 35 1505 Germacrene B 204 C ₁₅ H ₂₄ 7.70 36 1509 beta-bisabolene 204 C ₁₅ H ₂₄ 8.00 37 1516 γ-Amorphene 204 C ₁₅ H ₂₄ 16.49 38 1573 (Z) -3-hexen-1-yl benzoate 204 C ₁₃ H ₁₆ O ₂ 7.13 39 1587 Caryophyllene oxide 220 C ₁₅ H ₂₄ O 127.30 40 1646 t-Cadinol 222 C ₁₅ H ₂₆ O 7.75 41 1687 6-epi-a-Bisabolol 222 C ₁₅ H ₂₆ O 13.63 42 1770 Benzyl benzoate 212 C ₁₄ H ₁₂ O ₂ 101.76 43 1858 Phenethyl benzoate 226 C ₁₅ H ₁₄ O ₂ 7.21

²⁾ Retention index, ³⁾ MF = Molecular formula, ⁴⁾ MW = Molecular weight

8) Analysis of Volatile Flavor Components of Sweet Orange Oil and Search for Indicators

As a result of SPME analysis of sweet orange oil, a large amount of limonene was confirmed at 28.61 min.

The volatile fragrance component analysis of sweet orange oil is shown in Table 23 below.

No RI ²⁾ Compound name MF ³⁾ MW ⁴⁾ Mg / kg One 933 α-Pinene 136 C ₁₀ H ₁₆ 37.60 2 972 Sabinene 136 C ₁₀ H ₁₆ 18.59 3 989 β-Myrcene 136 C ₁₀ H ₁₆ 13.54 4 1005 1,3,5-Trimethylenecycloheptane 134 C ₁₀ H ₁₄ 41.86 5 1024 Mentha-1.4.8-triene 134 C ₁₀ H ₁₄ 29.77 6 1030 Limonene 136 C ₁₀ H ₁₆ 3407.92 I.S 1056 1-Butylbenzene 134 C _1O H ₁₄ - 7 1087 Methyl cyanoethanoate 99 C ₄ H ₅ NO ₂ 3.20 8 1090 4,? -Dimethylstyrene 132 C ₁₀ H ₁₂ 24.34 9 1100 Linalool 154 C ₁₀ H ₁₈ O 30.39 10 1113 2'-Methyl acetophenone 134 C ₉ H ₁₀ O 9.58 11 1134 cis-Limonene oxide 152 C ₁₀ H ₁₆ O 87.47 12 1138 trans-Limonene 1,2-epoxide 152 C ₁₀ H ₁₆ O 149.02 13 1180 Lavandulal 152 C ₁₀ H ₁₆ O 23.69 14 1189 trans-Isocarveol 152 C ₁₀ H ₁₆ O 15.60 15 1197 2,5,6-Trimethylhepta-1,3,6-triene 136 C ₁₀ H ₁₆ 24.55 16 1220 trans-Carveol 152 C ₁₀ H ₁₆ O 163.75 17 1233 cis-Carveol 152 C ₁₀ H ₁₆ O 74.34 18 1246 Carvone 150 C ₁₀ H ₁₄ O 276.47 19 1267 Rimantadine 179 C ₁₂ H ₂₁ N 3.72 20 1271 Isopiperitenone 150 C ₁₀ H ₁₄ O 13.96 21 1290 2,6-Dimethyl-1,7-octadiene-3,6-diol 170 C ₁₀ H ₁₈ O ₂ 11.16 22 1300 4-Octen-3-one 126 C ₈ H ₁₄ O 6.74 23 1332 Butanal 72 C ₄ H ₈ O 2.67 24 1346 lemonene-1,2-diol 170 C ₁₀ H ₁₈ O ₂ 35.43

²⁾ Retention index, ³⁾ MF = Molecular formula, ⁴⁾ MW = Molecular weight

9) Analysis of Volatile Flavor Components of Tea Tree Oil and Search for Indicators

As a result of analysis of the volatile flavor component analysis of tea tree oil using SPME and the volatile flavor component analysis of blending oil, three compounds that can be selected as the index component of tea tree oil were selected. The fragrance component of Peak 1 was identified as Terpinen-4-ol.

Table 24 and Table 25 show the results of volatile flavor component analysis of tea tree oil.

No RI ²⁾ Compound name MF ³⁾ MW ⁴⁾ Mg / kg One 932 α-Pinene 136 C ₁₀ H ₁₆ 141.46 2 977 β-Pinene 136 C ₁₀ H ₁₆ 37.37 3 989 β-Myrcene 136 C ₁₀ H ₁₆ 28.50 4 1006 α-Phellandrene 136 C ₁₀ H ₁₆ 19.37 5 1017 α-Terpinen 136 C ₁₀ H ₁₆ 297.55 6 1025 ρ-Cymene 134 C ₁₀ H ₁₄ 710.87 7 1029 Limonene 136 C ₁₀ H ₁₆ 75.29 8 1031 β-Phellandrene 136 C ₁₀ H ₁₆ 39.12 9 1032 Eucalyptol 154 C ₁₀ H ₁₈ O 241.39 I.S 1056 1-Butylbenzene 134 C ₁₀ H ₁₄ - 10 1059 γ-Terpinene 136 C ₁₀ H ₁₆ 837.13 11 1085 alpha-terpinolene 136 C ₁₀ H ₁₆ 150.00 12 1182 Terpinen-4-ol 154 C ₁₀ H ₁₈ O 4880.44 13 1187 p-Cymen-8-ol 150 C ₁₀ H ₁₄ O 18.44 14 1196 S-alpha-terpineol 154 C ₁₀ H ₁₈ O 440.41 15 1299 5,7-Octadien-3-ol, 2,4,4,7 182 C ₁₂ H ₂₂ O 14.43 16 1334 γ-Terpineol 154 C ₁₀ H ₁₈ O 11.28 17 1347 Terpinyl acetate 196 C ₁₂ H ₂₀ O ₂ 19.08 18 1364 3,3,5-Trimethylheptane-2,5-dione 170 C ₁₀ H ₁₈ O ₂ 10.52 19 1373 Isoledene 204 C ₁₅ H ₂₄ 25.84 20 1391 β-Elemene 290 C ₂₀ H ₃₄ O 8.90 21 1410 α-Gurjunene 204 C ₁₅ H ₂₄ 111.01 22 1423 β-Caryophyllene 204 C ₁₅ H ₂₄ 124.30 23 1431 Isolongifolene 204 C ₁₅ H ₂₄ 27.86 24 1438 α-Maaliene 204 C ₁₅ H ₂₄ 33.29 25 1442 Aromadendrene 204 C ₁₅ H ₂₄ 562.93

No RI ²⁾ Compound name MF ³⁾ MW ⁴⁾ Mg / kg 26 1450 5,11-Selinadiene 204 C ₁₅ H ₂₄ 73.83 27 1453 Cadina-3,5-diene 204 C ₁₅ H ₂₄ 24.40 28 1454 Valerena-4,7 (11) -diene 204 C ₁₅ H ₂₄ 16.67 29 1460 alpha-caryophyllene 204 C ₁₅ H ₂₄ 44.05 30 1464 9-Epicaryophyllene 204 C ₁₅ H ₂₄ 239.78 31 1490 δ-Selinene 204 C ₁₅ H ₂₄ 77.77 32 1494 Viridiflorene 204 C ₁₅ H ₂₄ 727.85 33 1499 (1E, 4E) -Germacrene B 204 C ₁₅ H ₂₄ 158.14 34 1500 α-Muurolene 204 C ₁₅ H ₂₄ 98.02 35 1507 Selin-4,7 (11) -diene 204 C ₁₅ H ₂₄ 12.75 36 1516 Germacrene D 204 C ₁₅ H ₂₄ 13.13 37 1521 δ-Cadinene 204 C ₁₅ H ₂₄ 752.95 38 1524 (Z) -Calamene 202 C ₁₅ H ₂₂ 168.87 39 1536 (E) -1,4-Cadinadiene 204 C ₁₅ H ₂₄ 125.97 40 1545 α-Calacorene 200 C ₁₅ H ₂₀ 20.00 41 1562 γ-Eudesmol 222 C ₁₅ H ₂₆ O 8.24 42 1567 Epiglobulol 222 C ₁₅ H ₂₆ O 82.70 43 1590 Globulol 222 C ₁₅ H ₂₆ O 296.09 44 1599 Viridiflorol 222 C ₁₅ H ₂₆ 0 174.28 45 1601 Cubeban-11-ol 222 C ₁₅ H ₂₆ O 127.88 46 1610 Ledol 222 C ₁₅ H ₂₆ O 38.87 47 1611 Rosifoliol 222 C ₁₅ H ₂₆ O 80.57 48 1616 10-epi-Cubebol 268 C ₁₅ H ₂₄ O ₄ 5.76 49 1632 1-epi-Cubenol 222 C ₁₅ H ₂₆ O 159.23 50 1636 Isospathulenol 220 C ₁₅ H ₂₄ O 70.10 51 1650 Torreyo 222 C ₁₅ H ₂₆ O 20.09

²⁾ Retention index, ³⁾ MF = Molecular formula, ⁴⁾ MW = Molecular weight

10) Analysis of Volatile Flavor Components and Composition of Surface Com

As a result of analyzing the volatile fragrance component analysis of soft white oil and the volatile fragrance component analysis of blending oil using SPME, one kind of compound which is highly likely to be selected as an indicator component of soft white oil was detected. Peak 1 was identified as Terpinyl acetate.

Table 26 and Table 27 show the results of analysis of the volatile flavor components of white cotton oil.

No RI ²⁾ Compound name MF ³⁾ MW ⁴⁾ Mg / kg One 922 Tricyclene 136 C ₁₀ H ₁₆ 15.09 2 926 α-Thujene 136 C ₁₀ H ₁₆ 130.40 3 933 α-Pinene 136 C ₁₀ H ₁₆ 301.58 4 949 Camphene 136 C ₁₀ H ₁₆ 69.19 5 973 Sabinene 136 C ₁₀ H ₁₆ 1515.51 6 977 (-) -? - Pinene 136 C ₁₀ H ₁₆ 50.54 7 989 β-Myrcene 136 C ₁₀ H ₁₆ 397.42 8 1006 α-Phellandrene 136 C ₁₀ H ₁₆ 10.50 9 1017 alpha-terpinene 136 C ₁₀ H ₁₆ 87.51 10 1025 o-Cymene 134 C ₁₀ H ₁₄ 330.99 11 1029 Limonene 136 C ₁₀ H ₁₆ 897.74 I.S 1056 1-Butylbenzene 134 C ₁₀ H ₁₄ - 12 1059 γ-Terpinene 136 C ₁₀ H ₁₆ 297.03 13 1071 trans-Sabinene hydrate 154 C ₁₀ H ₁₈ O 35.33 14 1086 alpha-terpinolene 136 C ₁₀ H ₁₆ 98.59 15 1182 Terpinen-4-ol 154 C ₁₀ H ₁₈ O 353.40 16 1187 p-Cymen-8-ol 150 C ₁₀ H ₁₄ O 11.60 17 1196 α-Terpineol 154 C ₁₀ H ₁₈ O 35.37 18 1286 Bornyl acetate 196 C ₁₂ H ₂₀ O ₂ 1206.74 19 1296 Methyl-myrtenate 180 C ₁₁ H ₁₆ O ₂ 17.98 20 1348 Terpinyl acetate 196 C ₁₂ H ₂₀ O ₂ 2674.31

No RI ²⁾ Compound name MF ³⁾ MW ⁴⁾ Mg / kg 21 1391 (-) -? - Elemene 204 C ₁₅ H ₂₄ 11.25 22 1423 β-Caryophyllene 204 C ₁₅ H ₂₄ 14.24 23 1440 cis-Thujopsene 204 C ₁₅ H ₂₄ 672.28 24 1449 3,5-Cadinadiene 204 C ₁₅ H ₂₄ 58.49 25 1466 cis-4,5-Muuroladiene 204 C ₁₅ H ₂₄ 362.48 26 1486 (-) -? - Chamigrene 204 C ₁₅ H ₂₄ 8.83 27 1499 Zonarene 204 C ₁₅ H ₂₄ 109.82 28 1507 (R) - [beta] -Himachalene 204 C ₁₅ H ₂₄ 50.34 29 1509 Pseudowriddene 204 C ₁₅ H ₂₄ 6.25 30 1516 (+) -? - Cadinene 204 C ₁₅ H ₂₄ 11.26 31 1521 (+) - delta-Cadinene 204 C ₁₅ H ₂₄ 150.41 32 1523 (-) - Calamenene 202 C ₁₅ H ₂₂ 64.19 33 1539 α-Cuprenene 204 C ₁₅ H ₂₄ 36.28 34 1551 Elemol 222 C ₁₅ H ₂₆ O 946.27 35 1612 Widdrol 222 C ₁₅ H ₂₆ O 8.05 36 1615 Cedrol 222 C ₁₅ H ₂₆ O 121.12 37 1619 di-epi-1,10-cubenol 222 C ₁₅ H ₂₆ O 28.53 38 1636 γ-Eudesmol 222 C ₁₅ H ₂₆ O 101.20 39 1660 α-Eudesmol 222 C ₁₅ H ₂₆ O 205.97 40 1906 Sclarene 272 C ₂₀ H ₃₂ 9.56 41 1945 Beyerene 272 C ₂₀ H ₃₂ 145.24

²⁾ Retention index, ³⁾ MF = Molecular formula, ⁴⁾ MW = Molecular weight

(5) Results of selection of indicator materials

Fatty acid analysis and volatile flavor component analysis were carried out on 10 kinds of raw oil contained in the composition of the present invention (blending oil), and analysis of 10 kinds of oil was finally conducted to present candidate substances corresponding to each oil .

Jojoba oil, calendula oil and sweet almond oil, which are the base oils of blending oil, have relatively low aroma components compared to essential oils. Therefore, it was concluded that the selection of indicator materials through fatty acid analysis is more effective than the fragrance component. (N-9), C22: 1 (n-9), and C22: 1 (n-9) in jojoba oil were analyzed by fatty acid analysis. And erucic acid were 73.28 and 13.78%, respectively, which were 38.92 and 7.32g / 100g, respectively. Although the content of eicosenoic acid was found to be 1.53g / 100g in calendula and 1.2g / 100g in sweet almond, it was found to be a relatively large value in jojoba oil. If the value is above a certain value, It was decided to do everything.

Calendula oil was the only C20: 2 (eicosadienoic acid) identified and selected as an indicator component of calendula oil. Isobornyl acetate, which was detected at 48.1 minutes due to its low content, was also selected as the indicator material.

Sweet almond oil contained the highest amount of fatty acids, but no specific materials were found through fatty acid analysis. As a result of screening various compounds, 3-carene was selected and blending oil was found to contain 4401.43 mg / g at 26.6 minutes.

Essential oil that forms blending oil is contained in a small amount compared with base oil, so we tried to select the indicator material by analyzing the fragrance component. In case of chamomile oil, bisabolol oxide A was observed at 76.8 min, and linalool was found at 34.3 min in jasmine. In Juniper berries, 1-terpineol was detected at 41.6 min and 495.64 mg / g in blending oil. Lavender oil was identified as two indicators and lavandulyl acetate and geranyl acetate were selected at 47.9 and 54.3 minutes. lavandulyl acetate and geranyl acetate were found in other oils, but the content of sweet orange oil was especially large and it was judged to be determined by using the range. Terpinen-4-ol was identified at 40.6 min for tea tree oil. The peaks of terpinyl acetate of 27.5 minutes were identified as the surface constituents of the white cotton oil.

Meanwhile, Table 28 shows the results of selecting the indicator candidate substances for each raw oil.

Raw material name content(%) Fatty acid analysis Fragrance component analysis Surface component Content (g / 100g) Characteristic Surface component content Characteristic Blending Raw material Blending Raw material Jojoba oil 45.579 C20: 1
(n-9)
(Eicosenoic acid) 18.65
(26.55%) 38.92
(73.28%) - C22: 1
(Erucic acid) 3.49
(4.97%) 7.32
(51.59%) Not edible,
Bio
diesel
Use Calendula oil 22.789 C20: 2
(Eicosadienoic acid) 0.04
(0.06%) 0.07
(0.08%) Isobornyl acetate 1131.50 0.91 [mu] g / g sweet
almond
oil 22.789 - 3-Carene 4401.43 3.78 ug / g Chamomile oil 0.457 - Bisabolol oxide A 81.24 126.26 mg / g Gout relief, inhibition of gastric ulcer progression, dermatitis treatment Jasmine oil 0.091 - Linalool 3473.65 303.00
Mg / g Relieving pain relief Juniper Berry Oil 1.823 - 1-Terpineol 495.64 22.67
Mg / g Nerve sedation,
Relieve insomnia Lavender Oil 1.823 - Lavandulyl acetate 401.98 96.92
Mg / g Anti-inflammatory,
Antimicrobial,
Anti-oxidant Geranyl acetate 164.35 709.11
Mg / g Use as soap fragrance sweet
Orange
oil 1.823 - Limonene 11621.27 3407.92
Mg / g Tea tree oil 0.912 - Terpinen-4-ol 3408.14 4880.44
Mg / g Anti-inflammatory,
Antimicrobial,
Immune function Textiles
oil 1.914 - Terpinyl acetate 713.72 2674.31
Mg / g

- Selection of the final indicator material of the composition (blending oil) of the present invention and the result of setting the reference value

Based on the results of the previous experiments, we selected the final index substances in the oil as a final blend of 10 selected raw oil oils, and selected 1 fatty acid and 3 fragrance components.

As a fatty acid, eicosenoic acid, C20: 1 (n-9, cis), which contains 26.55% of the total fatty acid content, was selected as a blending oil and the standard value was set to 18.0% or more.

Perfume components Bisphenol oxide A, lavandulyl acetate, and terpinen-4-ol were selected as compounds with high content of fragrance. Bisabolol oxide A is a representative fragrance ingredient of chamomile oil and has been reported to be effective in relieving gout, inhibiting the progress of gastric ulcer, and treating dermatitis.

lavandulyl acetate is an indicator component found in lavender oil in blending oil, 401.98 mg / g in blending oil, and is known to have anti-inflammatory, antibacterial and antioxidant properties.

terpinen-4-ol was detected as an index component of tea tree oil at 3408.14 mg / g and was reported to exhibit anti-inflammatory, antibacterial and immune functions. In case of fragrance component, the reproducibility is often deteriorated due to volatilization.

Meanwhile, Table 29 shows the result of selecting the indicator material of the final blending oil.

analysis Surface component Ingredient Name ingredient content Reference value Fatty acid (g / 100g) C20: 1 (n-9)
(Eicosenoic acid) 18.65
(26.55%) 18g / 100g or more Jojoba oil The fragrance component (mg / g) Bisabolol oxide A 81.24 Check whether Chamomile oil Lavandulyl acetate 401.98 Check whether Lavender Oil Terpinen-4-ol 3408.14 Check whether Tea tree oil

[Experimental Example 6] Analysis of β-Hexosaminidase secretion using Rat basophil RBL-2H3 CELL

(1) Analysis method

1) Measurement of cell proliferative capacity

To investigate the effect of the composition (blending oil) of the present invention on the cell proliferation and cytotoxicity of RBL-2H3 cells in order to measure anti-atopic activity, RBL-2H3 cells were seeded at 1 x 10 ⁴ cells / / well, and cultured in a 5% CO ₂ incubator at 37 ° C for 18 hours. (15.625-500 占 퐂 / ml) of each of the other blending oils having different composition ratios (9.5: 0.5, 9.0: 1.0, 8.5: 0.5, 8.0: 2.0) of the base oils and essential oils of Examples 1 to 4 Lt; / RTI > for 24 hours. Cell proliferation was measured using a microplate reader (Sunrise; Tecan Austria, Salzburg, Austria) using the CellTiter96Aqueous One Solution Assay of Cell Proliferation (Promega, Madison, WI, USA).

2) Measurement of secretion of β-Hexosaminidase

To analyze β-Hexosaminidase as an index for the anti-atopy inhibitory effect of blending oil on degranulation of RBL-2H3 cells, RBL-2H3 cells were seeded in 96 well plates at 2 × 10 ⁴ cells / 200 μl / well And cultured for 18 hours at 37 ° C in a 5% CO ₂ incubator. Cells of each well were washed twice with Siraganian buffer (119 mM NaCl, 5 mM KCl, 1 mM CaCl ₂ , 0.4 mM MgCl ₂ , 5.6 mM glucose, 25 mM PIPES, pH 7.2), and 160 μl of Siraganian buffer was added to each well And cultured for 10 minutes. Each blending oil having different composition ratios (9.5: 0.5, 9.0: 1.0, 8.5: 1.5, 8.0: 2.0) of the base oil and essential oil of Examples 1 to 4 was added to each concentration (15.625-500 g / And cultured for 1 hour. Then, the cells were further cultured for 1 hour under stimulation with PMA (50 nM) and A23187 (1 μM). After completion of the reaction on ice, 50 μl of the supernatant was transferred to a new 96-well plate and mixed with the same amount of substrate buffer (1 mM 4-ρ-nitrophenyl-N-acetyl-β-D-glucosaminide, 0.05 M sodium citrate, pH 4.5) And then reacted at 37 ° C for 1 hour. After that, 100 μl of stop solution (0.1 M NaHCO ₃ / 0.1 M Na ₂ CO ₃ , pH 10) was added to each well and the reaction was terminated. Absorbance was measured at 405 nm using a microplate reader (Sunrise; Tecan Austria).

(2) Analysis results

1) Evaluation of effect of blending oil on the cell proliferative capacity of RBL 2H3 cells by mixing ratio

In order to proceed with the anti-atopy test for evaluating the mixing ratio of the optimal blending oil, the cell proliferative ability of the blending oil by the mixing ratio to the RBL 2H3 cells was first evaluated. To evaluate the optimum mixing ratio, each blending oil prepared from the base oils and essential oil compositions of Examples 1 to 4 (9.5: 0.5, 9.0: 1.0, 8.5: 1.5, 8.0: 2.0) 500 / / ml), treated for 24 hours, and assessed for cell proliferation. As shown in the graph shown in Fig. 14, there was no cytotoxicity according to the indicated concentrations at all concentrations by mixing ratio.

2) RBL 2H3 cell anti-atopic effect of blending oil by mixing ratio

In order to analyze β-Hexosaminidase as an index for analyzing the anti-atopic effect of blending oil against inhibition of degranulation of RBL-2H3 cells, blending oil prepared at the optimum mixing ratio was evaluated. Each blending oil prepared in the mixing ratio (9.5: 0.5, 9.0: 1.0, 8.5: 1.5, 8.0: 2.0) of the base oil and the essential oil of Examples 1 to 4 was pre-treated with concentration (15.625-500 占 퐂 / , Degranulation was induced under stimulation with PMA (50 nM) and A23187 (1 μM). After 1 hour, as shown in the graph shown in FIG. 15, β-Hexosaminidase was quantitated in the cell culture supernatant. As a result, it was observed that the degranulation phenomenon was not inhibited in the 9.5: 0.5 blended oil, It was observed that degranulation was effectively inhibited in the blending oil, and degreasing was effectively suppressed in both the 8.5: 1.5 blend oil blend oil blend and the 8: 2 blending oil blend blend oil, so that the optimum mixing ratio of the blending oil was 9: 1 Respectively.

Claims (6)

70 to 95 wt% of a base oil consisting of 47.0 to 54.0 wt% of jojoba oil, 23.0 to 26.5 wt% of calendula oil and 23.0 to 26.5 wt% of sweet almond oil, Wow;
, Chamomile oil 0.44 - 13.9wt%, jasmine oil 0.75 - 1.76wt%, juniper berry oil 17.5 - 19.38wt%, lavender oil 17.5 - 19.38wt% Essential oil 5 composed of sweet orange oil 17.5 - 22.02 wt%, tea tree oil 8.85 - 10.57 wt% and chamaecyparis otbusa oil 24.0 - 26.45 wt% - 30 wt%, based on the total weight of the composition. The method according to claim 1,
The composition comprises, relative to the total weight of the composition,
0.1 to 5.0 parts by weight of at least one selected from animal oil, vegetable oil, wax and paraffin, 0.5 to 2.5 parts by weight of starch, 0.1 to 5.0 parts by weight of tragacanth, 0.1 to 3.0 parts by weight of a cellulose derivative, 0.1 to 5.0 parts by weight of bentonite, silica, talc, zinc oxide and titanium oxide. - 3.0 parts by weight of the composition is mixed and applied to the skin in the form of a cream. The method according to claim 1,
The composition comprises, relative to the total weight of the composition,
0.1 to 5.0 parts by weight of a carbomer, 0.1 to 1.0 part by weight of triethanolamine, 0.1 to 5.0 parts by weight of menthol, 0.1 to 5.0 parts by weight of ethanol, 0.1 to 10.0 parts by weight of disodium EDTA, 0.1 to 10.0 parts by weight of disodium EDTA and 0.1 to 2.0 parts by weight of methylparaben are applied to the skin in the form of gel (GEL) Composition for relieving and improving dermatitis. The method according to claim 1,
The composition comprises, relative to the total weight of the composition,
0.1 to 4.0 parts by weight of lactose, 0.1 to 2.0 parts by weight of talc or silica alone or a mixture thereof, 0.1 to 1.0 part by weight of aluminum hydroxides, calcium silicate (calcium silicate or polyamide powder or 0.1 to 5.0 parts by weight of the same is applied to the skin in the form of a powder.
The method according to claim 1,
The composition comprises, relative to the total weight of the composition,
0.1 to 10.0 parts by weight of purified water, 0.1 to 5.0 parts by weight of ethanol, 0.1 to 3.0 parts by weight of isopropanol, 0.1 to 3.0 parts by weight of ethyl carbonate, 0.1 to 5.0 parts by weight of ethyl acetate, 0.1 to 5.0 parts by weight of benzyl alcohol, 0.1 to 3.0 parts by weight of benzyl benzoate, 0.1 to 5.0 parts by weight of propylene glycol, 1,3-butylene glycol oil (1,3 0.1 to 5.0 parts by weight of polyethylene glycol or fatty acid ester of sorbitan, or 0.1 to 3.0 parts by weight of a mixture thereof, The composition for alleviating and improving atopic dermatitis according to claim 1, The method according to claim 1,
The composition comprises, relative to the total weight of the composition,
0.1 to 10.0 parts by weight of purified water, 0.1 to 5.0 parts by weight of ethanol or propylene glycol alone or a mixture thereof, 0.1 to 3.0 parts by weight of ethoxylated isostearyl alcohol, 0.1 to 3.0 parts by weight of polyoxyethylene sorbitol ester, 0.1 to 5.0 parts by weight of cellulose, 0.1 to 4.0 parts by weight of aluminum metahydoxide, 0.1 to 3.0 parts by weight of bentonite, agar or tragacanth or 0.1 to 2.0 parts by weight of a mixed component thereof is applied to the skin in the form of a suspension.

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