KR102172199B1 - Cosmetic composition for skin antiaging containing sodium dna and astazanthine - Google Patents

Abstract

The present invention relates to a skin anti-aging cosmetic, and more particularly, to a skin anti-aging cosmetic composition containing sodium DNA and astaxanthin. The cosmetic composition of the present invention contains sodium DNA and astaxanthin having whitening, anti-wrinkle and moisturizing effects, thereby imparting anti-aging properties to the skin and no skin irritation.

Description

Skin anti-aging cosmetic composition containing sodium DNA and astaxanthin as active ingredients {COSMETIC COMPOSITION FOR SKIN ANTIAGING CONTAINING SODIUM DNA AND ASTAZANTHINE}

The present invention relates to a cosmetic composition having an anti-aging effect on the skin, and more particularly, to a cosmetic composition comprising sodium DNA and astaxanthin, which has skin whitening, skin wrinkle improvement, and skin moisturizing effects.

The primary function of the skin is to protect the human body from external stimuli such as temperature, humidity and ultraviolet rays. In this way, when the skin is stimulated by strong ultraviolet rays, stress, and nutritional deficiencies from the outside, as the aging progresses, matrix components such as collagen and elastin are decomposed or decreased, resulting in a decrease in skin elasticity and keratinization.

As described above, aging is a phenomenon that occurs naturally in life over time, and currently, it is possible to slow or prevent the aging phenomenon from occurring through various methods, and most cosmetics focus on preventing such aging phenomenon. In recent years, as women's desire and interest in the function of cosmetics have increased considerably, high-functional products have appeared that have not only cleanliness and aesthetic functions, which are basic functions of cosmetics, but also whitening and moisturizing effect as bright and white skin, and improving and suppressing skin wrinkles. Was done. In particular, anti-aging products are of interest to many women who want to remain young, and accordingly, efforts to prevent and improve aging are being attempted. As a countermeasure, studies on various compounds are being conducted, among which sodium dienes (SODIUM DNA) and astaxanthin (astaxanthin) are compounds that are often treated as functional substances.

Sodium DNA is known as a tissue regeneration material and uses DNA extracted from salmon testis. Sodium DNA is used for wound treatment and tissue repair, but it is used at the discretion of the medical staff for some tissue regeneration or inflammation treatment that does not have an appropriate treatment, and it creates capillaries, improves blood circulation, anti-inflammatory action, and prevents capillary leakage. It is known as an ingredient that does. Recently, it has been developed as a skin-improving cosmetics having various functions such as acne scars, accident scars, stretch marks, wrinkles, and acne skin.

Astaxanthin (3,3'-dihydroxy-β,β'-carotene-4,4'-dione) is a non-vegetable pigment of the ketocarotenoid family that has a red color, and is a beta-carotene (β-carotene). ) Is a type of carotenoid pigment that has the same chemical structure as, and has one more hydroxyl group (-OH) and one ketone group (=O) at both end groups than beta-carotene as an antioxidant functional substance that removes harmful active oxygen. have.

Astaxanthin has significantly higher antioxidant activity than conventional antioxidants due to these unique molecular structural properties (500 times more than vitamin E and 20 times more than beta-carotene). Due to such high antioxidant activity, astaxanthin is widely used as a pharmaceutical, food additive, colorant, and feed additive for animals and fry, and its demand and range of application are expected to rapidly expand.

Astaxanthin is used for skin whitening (Korean Patent Laid-Open No. 10-2011-0013918), sodium DNA is anti-aging (Korean Patent Laid-Open No. 10-2011-0013918). The effect for each component is disclosed. However, it has not yet been addressed that the two ingredients are mixed in an appropriate composition ratio to lead to a significant difference in efficacy. In the present invention, to solve this.

The object of the present invention is to overcome the problems of conventional skin anti-aging cosmetic compositions such as low stability, side effects, low wrinkle improvement/whitening effect and high skin irritation of cosmetics, and excellent anti-aging effect, especially whitening, wrinkle improvement, and moisturizing effect. It is to provide a cosmetic composition.

The above object is to provide a cosmetic composition having an effect of skin anti-aging, whitening, wrinkle improvement, and moisturizing improvement, containing a mixture of sodium DNA and astaxanthin as an active ingredient.

Preferably, sodium DNA is contained in an amount of 0.001 to 5.0% by weight based on the total weight of the cosmetic composition, and astaxanthin is contained in an amount of 0.001% to 5.0% by weight based on the total weight of the cosmetic composition.

In addition, preferably, the cosmetic composition is a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, cleansing, oil, powder foundation, emulsion foundation, wax foundation, pack, massage cream and spray. It is characterized by having a formulation selected from the group consisting of.

Another object of the present invention is achieved by a cosmetic method using the cosmetic composition.

The cosmetic composition according to the present invention contains sodium DNA and astaxanthin as active ingredients, so that it has anti-aging effect on the skin and at the same time improves skin whitening, wrinkle improvement, and moisturizing while reducing skin irritation.

1 is a diagram showing the results of a skin wrinkle improvement test of the cosmetic composition of the present invention. The drawing on the left is before use, and the drawing on the right is 4 weeks after use.

All technical terms used in the present invention, unless otherwise defined, have the following definitions and correspond to the meanings as commonly understood by one of ordinary skill in the relevant fields of the present invention. In addition, although preferred methods or samples are described in the present specification, those similar or equivalent are included in the scope of the present invention.

The term “about” refers to 30, 25, 20, 15, 10, 9, 8, 7, 6, 5, or 30, 25, 20, 15, 10, 9, 8, 7, 6, 5, or relative to a reference amount, level, value, number, frequency, percentage, dimension, size, amount, weight or length. It means an amount, level, value, number, frequency, percentage, dimension, size, amount, weight or length varying by 4, 3, 2 or 1%.

Throughout this specification, unless otherwise required by the context, the terms “comprise” and “comprising” include the steps or components presented, or groups of steps or components, but any other step or component, or It is to be understood that a step or group of components is not excluded.

The present invention provides an anti-aging cosmetic composition containing sodium DNA and astaxanthine as active ingredients.

The present inventors discovered that as a result of measuring skin anti-aging, whitening effect, wrinkle improvement, and moisturizing effect through various experiments in order to study the applicability as a cosmetic containing sodium DNA and astaxanthin, the effect as a cosmetic can be expected. Became.

The cosmetic composition of the present invention comprising the above components is suitable as a cosmetic composition for skin anti-aging, whitening effect, wrinkle improvement, and moisturizing effect.

Preferably, the cosmetic composition comprising a mixture of sodium DNA and astaxanthin of the present invention is characterized in that it contains 0.001 to 5.0% by weight of sodium DAE and 0.001 to 5.0% by weight of astaxanthin in the total weight ratio.

Sodium DNA regenerates damaged skin cells and plays an anti-inflammatory action to revive skin cells and strengthen elasticity from the base layer.

Such sodium dienes may be extracted from natural products or may be used commercially.

The content of sodium DIA is preferably 0.001% to 5.0% by weight based on the weight ratio of the total composition.If it is less than the above range, the whitening function and skin regeneration function according to the sulfation effect of sodium DIA are poor, and it is not preferable that the content exceeds the above range. If it is not stable as a cosmetic composition, it is not preferable.

Astaxanthin has a very high antioxidant effect, so it reduces melanin pigments, that is, oxidized pigments in the skin, helps to maintain skin homeostasis and balance, has a whitening effect, and plays a role in helping cells regeneration along with sodium DNA.

Such astaxanthin may be extracted from natural products or any commercially available one may be used.

Particularly, the content of astaxanthin is preferably 0.001% to 5.0% by weight based on the weight ratio of the total composition, but if it is less than the above range, it is not preferable due to the lack of whitening function and skin regeneration ability due to antioxidant, and if it exceeds the above range Astaxanthin's intrinsic odor problem, as well as a cosmetic composition, is not stable, which is not desirable.

The mixture of sodium DINA and astaxanthin of the present invention may contain sodium DINA and astaxanthin in a weight ratio of 1:3 to 3:1, and preferably in a weight ratio of 1:1.

Ingredients included in the cosmetic composition of the present invention may include ingredients commonly used in cosmetic compositions in addition to the active ingredients as an active ingredient. For example, conventional ingredients such as antioxidants, stabilizers, solubilizers, vitamins, pigments and fragrances It may include an adjuvant, and a carrier.

The cosmetic composition of the present invention can be prepared in any formulation commonly prepared in the art, for example, solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing , Oil, powder foundation, emulsion foundation, wax foundation, pack, massage cream and spray may be formulated, but is not limited thereto. In more detail, it may be prepared in the form of a flexible lotion, nutritional lotion, nutritional cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.

When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, or zinc oxide may be used as carrier components. I can.

When the formulation of the present invention is a solution or emulsion, a solvent, a solubilizing agent or an emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 ,3-butylglycol oil, glycerol aliphatic ester, polyethyleneglycol or fatty acid ester of sorbitan.

When the formulation of the present invention is a suspension, liquid diluents such as water, ethanol or propylene glycol as carrier components, ethoxylated isostearyl alcohol, suspending agents such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystals Sex cellulose, aluminum metahydroxide, bentonite, agar or tracant, and the like can be used.

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder may be used as a carrier component. In particular, in the case of a spray, additionally chlorofluorohydrocarbon, propane / May contain propellants such as butane or dimethyl ether.

When the formulation of the present invention is a surfactant-containing cleansing, as a carrier component, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide ether Sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative or ethoxylated glycerol fatty acid ester, and the like may be used.

When the cosmetic composition of the present invention is a soap, a cleansing formulation containing a surfactant, or a cleansing formulation containing no surfactant, it may be applied to the skin and then wiped off, removed, or washed with water. As a specific example, the soap is liquid soap, powder soap, solid soap and oil soap, the surfactant-containing cleansing formulation is a cleansing foam, cleansing water, cleansing towel and cleansing pack, and the surfactant-free cleansing formulation is a cleansing cream , Cleansing lotion, cleansing water, and cleansing gel, but is not limited thereto.

On the other hand, the cosmetic method of the present invention refers to all cosmetic methods using the cosmetic composition of the present invention. That is, all methods known in the art using the cosmetic composition belong to the cosmetic method of the present invention.

The cosmetic composition of the present invention may be used alone or in duplicate, or may be used in conjunction with other cosmetic compositions other than the present invention. In addition, the cosmetic composition according to the present invention may be used according to a conventional method of use, and the number of times of use may be varied according to a user's skin condition or taste.

When a cosmetic method using a cosmetic composition containing sodium DNA and astaxanthin of the present invention as active ingredients is performed, skin anti-aging effect, whitening, wrinkle improvement and moisturizing effect can be obtained.

Hereinafter, the present invention will be described in more detail through examples. These examples are only for describing the present invention in more detail, and the scope of the present invention is not limited by these examples according to the gist of the present invention.

Preparation Example 1: Cosmetic composition containing sodium DNA and astaxanthin

A cosmetic composition containing sodium DNA and astaxanthin was prepared according to the description in Table 1 below.

ingredient Comparative Example 1 Comparative Example 2 Comparative Example 3 Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Sodium dna - 3 - 0.5 One 3 1.5 2.25 0.75 Astaxanthin - - 3 0.5 One 3 1.5 0.75 2.25 EDTA-2Na 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 Purified water to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100 glycerin 5 5 5 5 5 5 5 5 5 Cetostearyl alcohol 2 2 2 2 2 2 2 2 2 Glyceryl stearate 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 Microcrystalline 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 Squalane 5 5 5 5 5 5 5 5 5 Floating paraffin 3 3 3 3 3 3 3 3 3 Trioctanoine 5 5 5 5 5 5 5 5 5 Polysorbate 1.2 1.2 1.2 1.2 1.2 1.2 1.2 1.2 1.2 Sorbitan stearate 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Cyclomethicone 3 3 3 3 3 3 3 3 3 Incense, preservative Appropriate amount Appropriate amount Appropriate amount Appropriate amount Appropriate amount Appropriate amount Appropriate amount Appropriate amount Appropriate amount

Experimental Example 1: Measurement of whitening effect of a mixture of sodium DNA and astaxanthin

The whitening effect of each cosmetic composition of Comparative Examples 1 to 3 and Examples 1 to 6 was measured for 30 women aged 26-45 years with dark skin color. 7 locations of the subject's arm were displayed at 1 cm width by length, and the above-described cosmetics (Comparative Examples 1 to 3 and Examples 1 to 6) were applied to 7 locations, respectively. Cosmetics were applied twice a day for 2 months. Skin color (L value) was measured after 2 months using the measuring equipment Minolta CR 300, and the degree of change of skin color was calculated using Equation 1 below, and the results are shown in Table 2.

division Comparative Example 1 Comparative Example 2 Comparative Example 3 Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 ΔL value 0.53 1.25 1.19 1.38 2.35 3.87 3.07 2.04 2.08

As can be seen in Table 2, when sodium DNA and astaxanthin are used independently, it is difficult to impart a great effect on skin whitening, but when sodium DNA according to the present invention and astaxanthin are mixed and used, Comparative Examples 1 to 3 Compared to, it can be seen that Examples 1 to 6 exhibit excellent whitening effects in a concentration-dependent manner. In addition, it can be seen that Example 4 mixed at a mixing ratio of 1:1 exhibits a whitening effect superior to Examples 5 and 6.

Experimental Example 2: Skin irritation test of a mixture of sodium DNA and astaxanthin

In order to test the skin irritation of each cosmetic composition of Comparative Examples and Examples prepared above, a patch test was performed on 20 healthy adult men and women using a Haye's Test Chamber. The test site was wiped with 70% ethanol and dried, and then a chamber (Finn chamber, 100x10, EPITEST, Finland) in which 15 µl of the prepared test substance was dropped was sealed on the inside of the forearm of the test subject. After the patch was applied for 24 hours and the patch was removed, the test site was marked with a pen. After 1 hour and 24 hours, respectively (1 hour, 24 hours after patch removal = 24 hours, 48 hours including patch attachment), use a magnifying glass (8MC-150, DAZOR, United States of America) to observe the test site for erythema and edema. Was observed. The skin reaction was determined according to the regulations (Table 3) of the International Contact Dermatitis Research Group (ICDRG), and the average skin reactivity was calculated according to Equation 2 below, and the results are shown in Table 4.

sign Reactivity Evaluation standard - 0.0 Unresponsive, normal skin ± 0.5 Faint or mild erythema + 1.0 Definite but weak erythema, absence and papules ++ 2.0 Pronounced erythema, papules and vesicles +++ 3.0 Severe erythema and vesicle formation ++++ 6.0 Cannon formation

±+ ++ ±+ ++ (n=20) Comparative Example 1 --- --- 0.16 Comparative Example 2 --- --- 0.17 Comparative Example 3 --- --- 0.18 Example 1 --- --- 0.15 Example 2 --- --- 0.18 Example 3 --- --- 0.18 Example 4 --- --- 0.17 Example 5 --- --- 0.16 Example 6 --- --- 0.18

As can be seen from Table 4 above, it can be seen that the cosmetic compositions of Examples 1 to 6 containing a mixture of sodium DNA and astaxanthin according to the present invention do not have skin irritation.

Experimental Example 3: Skin wrinkle improvement test of a mixture of sodium DNA and astaxanthin

In this test, the cosmetic compositions of Comparative Examples 1 to 3 and Examples 1 to 6 were applied to the skin for evaluation of wrinkle improvement, and ANTERA 3D (Miravex, Ireland) was applied. The same test person measured the left eye wrinkle area of all subjects, and for reproducibility of the measurement, the same area was measured by overlapping the image measured before use of the test substance. The photographed image was matched using ANTERA CS software, which is an exclusive software for ANTERA 3D, and then the matched measurement part was used for analysis. Wrinkles small value indicating skin wrinkles was used for the analysis using the measurement variable Indentation index, and as the measured value decreases, it means that the wrinkles are improved. Instrument measurements were made before, after 2 weeks of use, and after 4 weeks of use. The results of the experiment are shown in Tables 5 and 6. The skin condition was checked at the time point after using the cosmetic of Example 2 for 4 weeks and shown in FIG. 1.

Average Before use Standard Deviation 2 weeks after use Standard Deviation After 4 weeks of use Standard Deviation Comparative Example 1 11.12 2.30 11.01 2.04 11.01 2.04 Comparative Example 2 10.96 2.51 10.94 2.14 10.86 2.01 Comparative Example 3 10.57 2.47 10.41 2.07 10.37 2.03 Example 1 10.66 2.71 9.71 2.09 9.66 1.97 Example 2 10.68 2.81 9.85 2.03 9.81 1.98 Example 3 10.91 2.88 9.64 2.07 9.60 1.99 Example 4 11.07 2.79 9.84 2.11 9.79 2.04 Example 5 10.67 2.65 9.77 2.09 9.69 2.03 Example 6 10.68 2.67 9.66 2.07 9.68 2.12

Improvement rate (%) 2 weeks after use After 4 weeks of use Comparative Example 1 1.34 1.69 Comparative Example 2 2.41 2.73 Comparative Example 3 2.51 2.71 Example 1 5.44 6.66 Example 2 7.74 8.13 Example 3 8.71 9.41 Example 4 7.98 8.51 Example 5 6.77 7.11 Example 6 6.98 7.56

As can be seen in Tables 5 and 6 and FIG. 1, it was confirmed that the cosmetic compositions of Examples 1 to 6 containing a mixture of sodium DNA and astaxanthin according to the present invention helped improve skin wrinkles. In addition, it can be seen that Example 4 mixed at a mixing ratio of 1:1 exhibits a better moisturizing effect than Examples 5 and 6.

Experimental Example 4: Skin moisturizing improvement test of a mixture of sodium DNA and astaxanthin

In this test, the cosmetic compositions of Comparative Examples 1 to 3 and Examples 1 to 6 were applied to the skin to evaluate the moisturizing improvement, and the moisturizing improvement was evaluated before, after 2 weeks of use, and after 4 weeks of use of the test substance using Epsilon E100.

As a result of analyzing the degree of moisturizing improvement in the right cheek area using Epsilon E100, the skin moisture showed a change of 29.44% after 2 weeks of use and 39.08% after 4 weeks of use compared to before using the test substance. In addition, it was statistically significant after 2 weeks of use and after 4 weeks of use compared to before use of the test substance (p<.001). It is judged that the test substance helps improve moisturization. Table 8 below shows the skin moisture change and Table 9 shows the skin moisture improvement rate.

Average Before use Standard Deviation 2 weeks after use Standard Deviation After 4 weeks of use Standard Deviation Comparative Example 1 16.34 3.2 18.34 4.1 19.41 3.5 Comparative Example 2 17.42 3.6 18.98 4.1 20.11 3.6 Comparative Example 3 18.51 3.4 18.99 3.9 19.87 3.5 Example 1 18.11 3.6 20.11 4.0 22.30 3.4 Example 2 19.02 3.7 24.95 4.0 26.81 3.6 Example 3 19.29 3.4 26.41 3.9 29.33 3.7 Example 4 18.98 3.5 23.11 4.1 25.49 3.6 Example 5 18.56 3.6 22.53 4.1 24.55 3.5 Example 6 18.53 3.5 22.04 4.0 24.37 3.6

Improvement rate (%) 2 weeks after use After 4 weeks of use Comparative Example 1 20.38 26.14 Comparative Example 2 23.41 27.11 Comparative Example 3 22.96 26.77 Example 1 27.41 31.44 Example 2 29.44 39.08 Example 3 31.11 39.87 Example 4 30.11 39.07 Example 5 28.34 33.08 Example 6 28.46 34.79

As can be seen in Tables 8 and 9, it was confirmed that it helps to improve skin moisturization through Examples 1 to 6 containing a mixture of sodium dienes and astaxanthin according to the present invention. In addition, it can be seen that Example 4 mixed at a mixing ratio of 1:1 exhibits a better moisturizing effect than Examples 5 and 6.

As the specific parts of the present invention have been described in detail above, it is clear that these specific techniques are only preferred embodiments for those of ordinary skill in the art, and the scope of the present invention is not limited thereto. Therefore, it will be said that the practical scope of the present invention is defined by the appended claims and their equivalents.

Claims (6)

A cosmetic composition for skin whitening containing a mixture of sodium DNA and astaxanthin in a weight ratio of 1:1 as an active ingredient. A cosmetic composition for improving skin wrinkles containing a mixture of sodium DNA and astaxanthin in a weight ratio of 1:1 as an active ingredient. A cosmetic composition for moisturizing skin containing a mixture of sodium DNA and astaxanthin in a weight ratio of 1:1 as an active ingredient. The method according to any one of claims 1 to 3,
The cosmetic composition containing 0.001 to 5.0% by weight of the sodium diena and astaxanthin based on the total weight of the cosmetic composition. The method of any one of claims 1 to 3, wherein the cosmetic composition is a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion. A cosmetic composition having a formulation selected from the group consisting of a foundation, a wax foundation, and a spray. delete

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