KR101879193B1 - Cosmetic Composition For Regenerating skin - Google Patents

Abstract

The present invention relates to a cosmetic composition for skin regeneration. According to the cosmetic composition, the composition is extracted from natural materials to be less likely to cause side effects to the skin, has the effect of regenerating the skin and enhancing the elasticity of the skin, and is made of the natural ingredients as the main ingredient so that strong skin regeneration can be realized without causing troubles to the sensitive skin. Therefore, the composition can be usefully used for manufacturing cosmetics for skin regeneration.

Description

[0001] Cosmetic Composition For Regenerating Skin [0002]

The present invention relates to a cosmetic composition for skin regeneration.

Skin aging is caused by internal aging and external aging. Internal aging is caused by decrease of various hormone secretion regulating metabolism, function of immune cell and deterioration of cell activity with age. External aging is caused by external factors such as ultraviolet rays and environmental pollution, Oxygen and the like.

When skin aging occurs, not only elasticity decreases but wrinkles increase, skin color becomes poor, skin troubles frequently occur, and spots, freckles, and black spots also increase.

Synthesis and degradation of extracellular matrix, such as collagen and elastin, are controlled in vivo, but their synthesis decreases as aging progresses, and matrix metalloproteinase (MMP), an enzyme that degrades collagen, degrades elastin The expression of the enzyme elastase, elastase, is promoted, and the elasticity of the skin is lowered and wrinkles are formed.

In addition, due to the increase of cyclooxygenase-2 (COX-2), an enzyme which produces proinflammatory cytokine in skin cells due to progress of aging or ultraviolet rays, prostaglandin E2 (Prostaglandin E2, PGE2) is increased and nitric oxide (NO) production is increased by iNOS (inducible nitric oxide synthase). These inflammatory reactions result in increased biosynthesis of substrate metalloproteinase (MMP) and collagen degradation.

Although adenosine and retinoic acid are known to be effective for improving skin wrinkles, adenosine has little clinical efficacy and retinoic acid can not be used in women who are pregnant, and there is a high possibility of irritation such as erythema. Accordingly, it is urgently required to develop a material capable of maintaining skin elasticity and regenerating skin.

Accordingly, the present inventors have developed an optimal composite composition for skin regeneration and elasticity enhancement, and proved its effect, thereby completing the present invention.

1. Korean Patent Registration No. 10-1550578

One object of the present invention is to provide a cosmetic composition for skin regeneration comprising sodium hyaluronate, aloe vera leaf extract and betaglucan as active ingredients.

One aspect of the present invention provides a cosmetic composition for skin regeneration comprising sodium hyaluronate, aloe vera leaf extract and beta-glucan as an active ingredient.

INDUSTRIAL APPLICABILITY The cosmetic composition for regeneration of skin of the present invention has a low effect of extracting from a natural material and having low risk of adverse effects on the skin, regenerating the skin and improving skin elasticity, and using natural ingredients as the main ingredients. Therefore, Skin regeneration can be implemented.

The aloe vera leaf extract used in the present invention may be either commercially available or directly extracted and used for extraction as follows.

The aloe vera leaves used for the extraction are washed with water to remove foreign matter, dried in the shade, and pulverized. A suitable amount of solvent is added to the pulverized aloe vera leaf powder so that it is completely immersed. The aloe vera leaf powder can be extracted using a conventional extraction solvent, and is preferably (a) an anhydrous or hydrated alcohol having 1 to 4 carbon atoms (e.g. methanol, ethanol, propanol, butanol, Propanol and n-butanol), (b) a mixed solvent of the lower alcohol and water, (c) acetone, (d) ethyl acetate, (e) chloroform, (f) 1,3- butanol, propanol, butylene glycol, propylene glycol, chloroform, ethyl acetate, isopropanol, butanol, isopropanol, butanol, It is preferable to use dichloromethane, a nucleic acid, petroleum ether or diethyl ether.

According to one embodiment of the present invention, the cosmetic composition may further include saccharide isomerate, nordihydroguaiaretic acid (NDGA), and oleanolic acid .

According to one embodiment of the present invention, the composition comprises 0.01 to 10.0% by weight of sodium hyaluronate; 0.01 to 10.0% by weight of aloe vera leaf extract; And 0.01 to 2.0% by weight of beta-glucan.

According to one embodiment of the present invention, the composition comprises 0.01 to 10.0% by weight of saccharide isomerate; 0.01 to 10.0% by weight of nordihydroguaiaretic acid; And 5% by weight of oleanolic acid.

According to one embodiment of the present invention, sodium hyaluronate, aloe vera leaf extract and beta glucan can be mixed in a weight ratio of 1: 0.5: 1 to 1: 0.8: 1.5, preferably 1: 0.6: 1.2 By weight.

According to one embodiment of the present invention, the functional cosmetic composition may exhibit any one or more effects selected from the group consisting of skin regeneration, skin elasticity enhancement and wrinkle improvement.

According to one embodiment of the present invention, the composition may be contained in an amount of 0.01 to 30.0 parts by weight based on 100 parts by weight of cosmetics.

According to one embodiment of the present invention, the cosmetic composition may be in the form of a solution, a mist, a suspension, an emulsion, a paste, a gel, a cream, a lotion, a powder, a soap, a surfactant-containing cleansing oil, a powder foundation, A wax foundation, and a spray.

More specifically, it can be manufactured in the form of a mist, a flexible lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an ampoule, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide can be used as the carrier component have.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide ether Sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives, or ethoxylated glycerol fatty acid esters.

When the cosmetic composition of the present invention is a soap, a surfactant-containing cleansing agent, or a surfactant-free cleansing agent, it may be applied to the skin and then wiped off or removed or washed with water. The surfactant-containing cleansing formulation is a cleansing foam, a cleansing water, a cleansing towel, and a cleansing pack. The surfactant-free cleansing formulation may be a cleansing cream, , Cleansing lotion, cleansing water and cleansing gel, but is not limited thereto.

The ingredients contained in the cosmetic composition of the present invention include, in addition to the above-mentioned effective ingredients, the ingredients conventionally used in cosmetic compositions, such as antioxidants, stabilizers, solubilizers, vitamins, And a carrier.

INDUSTRIAL APPLICABILITY The cosmetic composition for regeneration of skin of the present invention has a low effect of extracting from a natural material and having low risk of adverse effects on the skin, regenerating the skin and improving skin elasticity, and using natural ingredients as the main ingredients. Therefore, Skin regeneration can be realized, so that it can be usefully used for manufacturing cosmetics for skin regeneration.

Fig. 1 is a photograph of the cosmetic composition of the present invention stored at 35 ° C. (A) shows immediately after storage, and (B) shows 6 weeks after storage.
2 is a graph showing the cell regeneration rate of the cosmetic composition of the present invention.

Hereinafter, one or more embodiments will be described in more detail by way of examples. However, these embodiments are intended to illustrate one or more embodiments, and the scope of the present invention is not limited to these embodiments.

Example  1 to 2 and Comparative Example  1-4. Cosmetics  Composition manufacturing

Sodium hyaluronate, aloe vera leaf extract, betaglucan, saccharide isomerate, nordihydroguaiaretic acid and oleanolic acid were prepared by purchasing commercially available products.

Composite compositions were prepared by varying the constituent components of each of the examples and comparative examples as shown in Table 1 below.

division Constituent Example 1 5% by weight of sodium hyaluronate + 3% by weight of aloe vera leaf extract + 6% by weight of beta-glucan, Example 2 Example 1 + 5% by weight of + saccharide isomerate + 0.5% by weight of nordihydroguaiaretic acid + 5% by weight of oleanolic acid [ Comparative Example 1 Purified water 10 wt% Comparative Example 2 5% by weight of sodium hyaluronate + 5% by weight of beta-glucan, Comparative Example 3 1.5% by weight of aloe vera leaf extract + 5% by weight of beta-glucan, Comparative Example 4 5% by weight of sodium hyaluronate + 1.5% by weight of aloe vera leaf extract

Thereafter, the composite composition was mixed with purified water, betaine, carbomer, clophenethine, phage-60 almond glyceride, phage-60 hydrogenated castor oil, phenoxyethanol, caprylic glycol, allantoin, bacillus / Examples 1 and 2 and Comparative Examples 1 to 4 ampoules were prepared by blending glutamic acid fermented filtrate, hydroxyethyl cellulose and glycosyltrehalose raw materials.

Comparative Example  5-7. Cosmetics  Composition manufacturing

Comparative Examples 5 to 7 were almost the same as those of Examples 1 and 2, but the ampoules were prepared in different ratios of components as shown in Table 2 below.

division Constituent Comparative Example 5 6% by weight of sodium hyaluronate + 1% by weight of aloe vera leaf extract + 7% by weight of beta-glucan, Comparative Example 6 7% by weight of sodium hyaluronate + 5% by weight of aloe vera leaf extract + 2% by weight of beta-glucan, Comparative Example 7 1% by weight of sodium hyaluronate + 5% by weight of aloe vera leaf extract + 8% by weight of beta-glucan,

Experimental Example 1 . Skin stability evaluation

The ampules prepared in Examples 1 and 2 were applied to the lower limbs of 20 healthy male and female (10 males and 10 females), and the human skin test was carried out. The habock of the subject was thoroughly wiped with 70% ethanol, and the ampoule was closed for 24 hours. After 24 hours, the adhesive tape and the pin chamber were removed, and the sample remaining on the skin with gauze was lightly wiped off and the skin condition was observed.

As a result, in the ampoule containing the composite composition according to the present invention, skin irritation was not confirmed and it was confirmed that there was no irritation to human skin.

Experimental Example 2 . Formulation stability evaluation

The stability of the ampule composition containing the cosmetic composition of the present invention was evaluated. The compositions of Examples 1 and 2 were stored at 35 < 0 > C for 6 weeks. Then, the stability of the composition was evaluated by checking the color of the ampoule composition and the degree of precipitation.

As a result, it was determined that the ampoule composition containing the composite composition did not change color after 6 weeks of storage, no precipitate was formed, and there was no problem in stability (Fig. 1).

Experimental Example  3. Skin elasticity evaluation

Twenty healthy male and female adults (10 males and 10 females) were divided into two groups, and the ampules of Example 2 and Comparative Example 1 were applied to both sides of the face for a predetermined amount every three hours every day. One week later, the elasticity of the skin was evaluated by measuring the disappearance time of the marks by applying a force of about 5 N to a cylindrical hollow having a diameter of 10 mm.

As a result, it was confirmed that the elasticity of the experimental group of Example 2 was increased to about 17% level as compared with Comparative Example 1. Therefore, it was confirmed that the use of an ampoule containing the composite composition of the present invention has an effect of improving skin elasticity.

Experimental Example  4. Skin regeneration test

A wound healing assay was performed to confirm skin regeneration effect.

Specifically, keratinocyte (HACAT) was adjusted to 5 × 10 ⁵ cells / well using DMEM supplemented with 10% FBS, and then inoculated on a 6-well plate and cultured for 18 hours. The cells grown on the 6 well plate were scratched to remove a portion of the cells from the bottom of a 6-well plate, and the ampoule samples of Examples 1 and 2 and Comparative Examples 1 to 7 were treated and cultured for 18 hours. The degree of regeneration of the cells was confirmed, and the results are shown in Table 3 and FIG. Cell regeneration was calculated by the following equation (1), and hyaluronic acid was used as a positive control.

As shown in FIG. 2 and Table 3, it was confirmed that the skin regeneration ability of Examples 1 and 2 was superior to that of the control group.

division Cell viability (%) Example 1 89.5% Example 2 92.2% Comparative Example 1 50.1% Comparative Example 2 75.1% Comparative Example 3 71.0% Comparative Example 4 78.5% Comparative Example 5 80.3% Comparative Example 6 81.1% Comparative Example 7 82.9% Positive control group 78.5%

Experimental Example  5. Evaluation of Skin Barrier Strength

Subjects were randomly divided into two groups of 20 adult women aged 20 to 40 years. For two weeks, Example 2 and Comparative Example 1 according to one embodiment of the present invention were used once each day to a predetermined test site inside the lower portion Transepidermal water loss (TEWL) was measured.

The pathogenesis of the skin barrier progresses under the influence of various regulatory factors that control the proliferation and final differentiation of keratinocytes. TEWL is an important physiological indicator reflecting the stratum corneum function in the damage and recovery of skin barrier. In addition, TWEL is an important adjunct to assess the barrier function of the skin, allowing objective assessment of the healing process of atopic or dry skin. Generally, TEWL is increased when damage occurs to the stratum corneum. That is, an increase in TWEL means that the function of the stratum corneum is lowered and the skin barrier function is impaired. In addition, light-skin moisture loss is the most commonly used method of examining skin barrier function.

Specifically, the amount of water loss in the epidermis was measured using a Vapometer (Delfin). The measurement was performed once, and the measurement was performed for about 10 seconds. The lower the value of TEWL, the better the skin condition. TEWL, which is an index of skin water barrier function and predicted skin water barrier ability, is expressed as the amount of water loss (g / hm ² ) through the skin.

In addition, changes in the amount of water loss in the temporal epidermal space were measured by the repeated measures ANOVA (Bonferroni correction) and the non-parametric Friedman test (Wilcoxon signed rank test, Bonferroni correction) Respectively. The differences between the test site use and non-canvas sites were determined by the independent method t-test, which is a parametric method, and the Mann-Whitney U test, which is a non-parametric method.

As a result, as shown in the following Table 4, it was confirmed that in Example 2, the amount of water hardness loss was decreased and the skin barrier was strengthened as compared with Comparative Example 1.

division Example 2 [ Comparative Example 1 [ Light skin moisture loss 10.98 ± 2.84 14.67 ± 2.21 TWEL variation -17.80 + 4.42 -11.50 ± 2.43 Skin barrier intensification rate 62.86 + - 10.58 51.35 ± 11.48

Experimental Example  6. Skin Cosmetic  Clinical trial

The cosmetic compositions prepared according to Examples 1 and 2 and Comparative Examples 1 to 7 were subjected to sensory evaluation for moisture content, skin texture uniformity and pore size.

Specifically, the subjects were 20 adult women aged 20 to 40 years. The cosmetic composition was applied to the face balls of a subject 5 cm in diameter three times a day, respectively, for 7 days, The feeling for the compositions of Comparative Examples 1 to 7 was evaluated according to the evaluation criteria described below, and the results were expressed as average values. The items of evaluation were aimed at dullness, freshness, absorbency, moisturizing, nutritional and overall satisfaction after use, and the evaluation criteria are as follows. In Table 5, the higher the moisturizing degree is, the better, and the lower the skin texture uniformity is, the lower the skin texture uniformity is, and the smaller the pore size and the degree of deposition of pigment are, the better. The value means a steady state without skin irritation.

Evaluation items Standard Poor normal good Degree of moisturizing 33 0-25 26-65 66-100 Skin texture uniformity 60 100-75 74-35 34-0 Pore size 48 100-75 74-35 34-0

As a result of the evaluation, as shown in the following Table 6, in the experimental group using the cosmetic compositions of Examples 1 and 2 of the present invention, the moisture of the skin was increased and the moisturization was excellent, the roughness of the skin was reduced, , The size of the pores decreased, and the degree of melanin pigment deposition decreased, thereby confirming the whitening effect.

division Degree of moisturizing Skin texture uniformity Pore size 1 day 7 days 1 day 7 days 1 day 7 days Comparative Example 1 27 35 68 61 72 72 Comparative Example 2 26 35 69 60 72 70 Comparative Example 3 27 36 68 62 69 69 Comparative Example 4 29 39 67 59 70 67 Comparative Example 5 30 45 68 60 72 58 Comparative Example 6 32 46 66 58 71 55 Comparative Example 7 31 46 69 59 69 57 Example 1 36 53 65 54 71 44 Example 2 35 55 61 49 69 46

Accordingly, it has been confirmed that when the cosmetic composition of the present invention is used, it serves as a protective film for supplying moisture to the skin and preventing evaporation of moisture on the skin surface, thereby reducing roughness and preventing skin dryness. In addition, it was confirmed that the pore improvement and the whitening effect by inhibition of the melanin pigment were also excellent.

The present invention has been described with reference to the preferred embodiments. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims. Therefore, the disclosed embodiments should be considered in an illustrative rather than a restrictive sense. The scope of the present invention is indicated by the appended claims rather than by the foregoing description, and all differences within the scope of equivalents thereof should be construed as being included in the present invention.

Claims (8)

Sodium hyaluronate, aloe vera leaf extract, beta-glucan, saccharide isomerate, nordihydroguaiaretic acid (NDGA), and oleanolic acid (oleanolic acid) as an active ingredient,
The proportion of the active ingredient is 4 to 6% by weight of sodium hyaluronate; 2 to 4% by weight of aloe vera leaf extract; 5 to 7% by weight of beta-glucan; 4 to 6% by weight of saccharide isomerate; 0.3 to 0.7% by weight of nordihydroguaiaretic acid; And 5% by weight of oleanolic acid. delete delete delete delete The cosmetic composition for skin regeneration according to claim 1, wherein the cosmetic composition exhibits one or more effects selected from the group consisting of skin regeneration, skin elasticity enhancement and wrinkle improvement. The cosmetic composition for skin regeneration according to claim 1, wherein the composition is contained in an amount of 0.01 to 30.0 parts by weight based on 100 parts by weight of the cosmetic. The cosmetic composition according to claim 1, wherein the cosmetic composition is a solution, a mist, a suspension, an emulsion, a paste, a gel, a cream, a lotion, a powder, a soap, a surfactant-containing cleansing oil, a powder foundation, an emulsion foundation, Wherein the composition is at least one selected from the group consisting of water, ethanol, and water.

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