KR102171980B1 - A cosmetic composition for improving skin condition - Google Patents

Abstract

The present invention relates to a cosmetic composition for skin improvement, and more specifically, to a cosmetic composition for skin improvement comprising a gold and silver extract. The gold and silver extract of the present invention can be used as a cosmetic material because it has the effect of regenerating cells and improving skin wrinkles through cell regeneration, fat accumulation, and inhibition of autophagy in skin cells.

Description

A cosmetic composition for improving skin condition

The present invention relates to a cosmetic composition for skin improvement, and more specifically to a cosmetic composition for skin improvement comprising a gold and silver extract.

Due to the development of science, various excellent ingredients and materials have been developed and the effect on the skin is increasing, and as a result, cosmetic products are being developed that are effective in skin regeneration and wrinkle improvement. Currently, cosmetics for skin regeneration and wrinkle improvement have been released with collagen and elastin as the main targets, and research has been conducted targeting them, but the competitiveness is gradually falling because new technologies are not currently being developed. In addition, aging progresses, and due to the reduction of fat in the skin, the skin is turned off and the elasticity decreases. At this time, a method of restoring skin elasticity through a fat graft procedure has been recently performed, but various side effects such as facial asymmetry, foreign body sensation, and pain have been reported.

Lonicera japonica refers to the buds of honeysuckle vines in the family Honeysuckle. It is known to have antioxidant and anti-inflammatory effects, so it is a natural product with many papers and patents proving its anti-inflammatory and antioxidant efficacy. In the existing technology, the effects of skin regeneration and wrinkle improvement such as the protective effect of gold and silver coins against keratinocyte formation damage, atopic dermatitis improvement effect, skin whitening effect, skin wrinkle improvement, itchiness improvement, and skin aging prevention have been revealed.

Therefore, it was attempted to develop a technology with new skin regeneration and anti-wrinkle effects, not previously known technology, using gold and silver coins.

Korean Patent Registration No. 10-1515443 Japanese Patent Application Publication No. 2009-269900

Accordingly, the present inventors prepared an extract from gold and silver flowers in order to discover natural products that are effective in skin regeneration and wrinkle improvement, and the prepared gold and silver flower extract induces cell regeneration and fat accumulation in a concentration-dependent manner in NIH3T3 cells, which are skin cells. The present invention was completed by confirming the excellent effect on accumulation, and by confirming the regeneration and wrinkle improvement efficacy of skin cells through skin elasticity-related factors while suppressing autophagy progressed by skin aging.

Accordingly, it is an object of the present invention to provide a cosmetic composition for improving skin comprising a gold and silver extract as an active ingredient.

In order to achieve the object, according to an embodiment of the present invention, there is provided a cosmetic composition for improving skin comprising an extract or a fraction thereof as an active ingredient.

Here, the extract may be extracted with one or more extraction solvents selected from the group consisting of water, an alcohol having 1 to 4 carbon atoms, a polyhydric alcohol, a hydrocarbon-based solvent, or a mixed solvent thereof.

In addition, the extract is characterized in that it increases the regeneration of skin cells or the accumulation of fat in the skin cells.

In addition, the extract is characterized in that it promotes the regeneration of skin cells by inhibiting autophagy.

In addition, the extract is characterized by increasing collagen and reducing MMP1 or MMP3.

In addition, the extract may be contained in an amount of 0.01 to 20% by weight based on the total weight of the cosmetic composition.

Here, the cosmetic composition is a flexible lotion, gel, water-soluble liquid, milk lotion, nutrition cream, massage cream, essence, oil-in-water emulsion, water-in-oil emulsion, anhydrous face product, solid anhydrous product, aqueous phase using globules Oil dispersion, ionic fat vesicles, nonionic fat vesicles, ointment, cleansing foam, cleansing water, pack, body oil, oil-in-water makeup base, water-in-oil makeup base, foundation, skin cover, lipstick, lip gloss, It may be a formulation selected from the group consisting of face powder, two-way cake, eye shadow, mascara, cheek color, and eyebrow pencil.

In addition, according to another embodiment of the present invention, extracting gold and silver coins by introducing them into a reactor containing an extraction solvent; And filtering the extracted extract and removing the solvent from the filtrate using a rotary vacuum concentrator.

Here, the extraction solvent may be one or more selected from the group consisting of water, an alcohol having 1 to 4 carbon atoms, a polyhydric alcohol, a hydrocarbon-based solvent, or a mixed solvent thereof.

According to the present invention as described above, the gold and silver extract of the present invention has the effect of regenerating cells and improving skin wrinkles through cell regeneration, fat accumulation, and autophagy inhibition in skin cells. Can be used as.

The effects according to the present invention in the above are not limited to the above, and other effects of the present invention are clearly described by those of ordinary skill in the field to which the present invention belongs through the matters described in the following examples and claims. It will be understandable.

Figure 1 shows the skin improvement effect of the gold and silver extract of the present invention.
Figure 2 shows the cell proliferation effect for each solvent of the gold and silver extract of the present invention.
Figure 3 shows the cell proliferation effect for each solvent of the gold and silver fraction of the present invention.
Figure 4 shows the fat accumulation effect for each solvent of the gold and silver extract of the present invention.
5 shows the fat accumulation effect for each solvent of the gold and silver fractions of the present invention.
Figure 6 shows the cell proliferation effect of the extract of gold and silver per ethanol concentration of the present invention.
Figure 7 shows the fat accumulation effect of the gold and silver extracts according to the ethanol concentration of the present invention.
Figure 8 shows the results of comparing the cell proliferation and fat accumulation of insulin with the gold and silver extract of the present invention.
Figure 9 shows the results of Western blot autophagy related factors of the extract of the present invention.
Figure 10 shows the ratio of the autophagy-related LC3-II and I of the gold and silver extract of the present invention.
Figure 11 shows the cell proliferation and fat accumulation effect of autophagy-induced cells of the extract of the present invention.
Figure 12 shows the effect of increasing the collagen expression of the gold and silver extract of the present invention.
Figure 13 shows the effect of reducing the expression of MMP1 and MMP3 of the gold and silver extract of the present invention.

Hereinafter, the present invention will be described in more detail.

The present invention relates to a cosmetic composition for skin improvement, and more specifically to a cosmetic composition for skin improvement comprising a gold and silver extract.

According to an embodiment of the present invention, there is provided a cosmetic composition for improving skin comprising an extract or a fraction thereof as an active ingredient.

Here, the extract may be extracted with one or more extraction solvents selected from the group consisting of water, an alcohol having 1 to 4 carbon atoms, a polyhydric alcohol, a hydrocarbon-based solvent, or a mixed solvent thereof. However, as a solvent for extracting the extract from the gold and silver flower, any organic solvent allowed in cosmetics may be used, but for example, purified water, methanol, ethanol, propanol, isopropanol ( isopropanol), alcohol having 1 to 4 carbon atoms including butanol, acetone, ether, benzene, chloroform, ethyl acetate, methylene chloride ), various solvents such as hexane and cyclohexane may be used alone or in combination. Preferably, ethanol (alcohol) may be used, and more preferably, 30 to 100% ethanol may be used.

As the extraction method, any one of methods such as hot water extraction, cold precipitation extraction, reflux cooling extraction, solvent extraction, steam distillation method, ultrasonic extraction method, elution method, and compression method may be used. In addition, the desired extract may be further subjected to a conventional fractionation process, or may be purified using a conventional purification method. There is no limitation on the method for preparing the gold and silver extract of the present invention, and any known method may be used.

For example, the gold and silver extract included in the composition of the present invention may be prepared in a powder state by an additional process such as distillation under reduced pressure and freeze drying or spray drying of the primary extract extracted by the solvent extraction method described above. In addition, the primary extract was further purified using various chromatography such as silica gel column chromatography, thin layer chromatography, high performance liquid chromatography, etc. You can also get it.

Therefore, in the present invention, the gold and silver flower extract is meant to include all extracts, fractions and purified products obtained in each step of extraction, fractionation, or purification, and their dilutions, concentrates, or dried products.

The gold and silver flower extract prepared in the present invention can increase the regeneration of skin cells or the accumulation of fat in the skin cells, and also inhibit autophagy to promote the regeneration of skin cells. In addition, the extract may increase collagen and decrease MMP1 or MMP3 expression, thereby increasing skin elasticity.

In the present invention, the extract may be contained in an amount of 0.01 to 20% by weight based on the total weight of the cosmetic composition.

In one embodiment of the present invention, the gold and silver extract may be included in the cosmetic composition at a concentration of 1 μg/ml to 1000 μg/ml (or 1 to 1000 ppm).

Here, the cosmetic composition is a flexible lotion, gel, water-soluble liquid, milk lotion, nutrition cream, massage cream, essence, oil-in-water emulsion, water-in-oil emulsion, anhydrous face product, solid anhydrous product, aqueous phase using globules Oil dispersion, ionic fat vesicles, nonionic fat vesicles, ointment, cleansing foam, cleansing water, pack, body oil, oil-in-water makeup base, water-in-oil makeup base, foundation, skin cover, lipstick, lip gloss, It may be one formulation selected from the group consisting of face powder, two-way cake, eye shadow, mascara, cheek color, and eyebrow pencil.

Products to which the cosmetic composition of the present invention can be added include, for example, astringent lotion, softening lotion, nutrient lotion, various creams, essences, packs, and cosmetics such as foundations, cleansing, face wash, soap, treatment, beauty liquid Etc.

According to a preferred embodiment of the present invention, the content of the active ingredient (gold and silver extract) of the present invention is 0.00001 to 40% by weight, preferably 0.0005 to 40%, and more preferably 0.01 to 20% by weight based on the total weight of the composition. %to be. If the content of the active ingredient (gold and silver extract) is less than 0.00001% by weight, the effect of improving skin wrinkles is greatly reduced, and if it exceeds 40% by weight, it may cause skin irritation, and a problem in the formulation may occur.

In the present invention, the cosmetic composition according to an embodiment is an additional auxiliary or additive material, such as a mild surfactant, an oil body, an emulsifier, a hyperfatting agent, a pearl luster wax, and a consistency substance. ), thickeners, polymers, silicone compounds, fats, waxes, stabilizers, biologically active substances, deodorants, dandruff inhibitors, film formers, swelling agents, antioxidants, inorganic coloring pigments, hydrophobic substances, preservatives, insect repellents, magnetic tannins It may contain self tanning agents, solubilizing agents, flavoring oils, coloring agents, and the like.

Specific examples of the formulation of the cosmetic composition using the gold and silver extract according to an embodiment of the present invention are shown below. However, the following formulation is intended to embodi the present invention, and will not be limited thereto.

Formulation Example 1. Cream

Raw material Content (%, w/w) Gold and silver extract 5.0 Caprylic/Capric Triglyceride 7.0 Shea Butter 2.0 Tocopheryl acetate 0.6 Stearic acid 2.0 Cetearyl Alcohol/Polysorbate 60 4.0 Xanthan gum 0.1 glycerin 3.0 1,3-butylene glycol 3.0 Triethanolamine 0.5 antiseptic a very small amount Purified water Balance

Formulation Example 2. Lotion

Raw material Content (%, w/w) Gold and silver extract 5.0 Caprylic/Capric Triglyceride 6.0 Tocopheryl acetate 0.4 Cetearyl Alcohol/Ceteareth-20 3.0 Glyceryl Stearate/Pig-100 Stearate 3.0 Xanthan gum 0.2 glycerin 2.0 1,3-butylene glycol 3.0 antiseptic a very small amount Purified water Balance

Formulation Example 3. Spray

Raw material Content (%, w/w) Gold and silver extract 5.0 Butyl octyl salicylate 5.0 Tocopheryl acetate 1.0 Acrylate/Octylacrylamide Copolymer 3.0 Ethyl alcohol Balance

Formulation Example 4. Stick

Raw material Content (%, w/w) Gold and silver extract 5.0 Butyl octyl salicylate 5.0 Tocopheryl acetate 1.0 Beeswax 22.0 Sorbitanolivate 10.0 Silica 5.0 antiseptic a very small amount Isopropyl Myristate Balance

On the other hand, according to another embodiment of the present invention provides a method for producing a gold and silver extract comprising a; step of extracting by introducing the gold and silver coins into a reactor containing an extraction solvent.

Here, as the extraction solvent, the extraction solvent used in the cosmetic composition disclosed above may be used in the same manner.

More specifically, dried gold and silver coins are extracted with reflux cooling using 70% ethanol as an extraction solvent.

In addition, filtering the extracted extract, and removing the solvent using a rotary vacuum concentrator for the filtrate; can be prepared including a gold and silver extract.

The gold and silver extract may be further subjected to an additional fractionation process using ethanol, n-hexane, chloroform, or water to obtain a gold and silver extract or fraction having high purity, and may preferably be an ethanol fraction, but is limited thereto. It does not become.

Fig. 1 shows the skin improvement effect of the gold and silver extract of the present invention. According to FIG. 1, the extract of gold and silver flowers prepared in the present invention has the effect of regenerating cells and improving skin wrinkles through inhibition of cell proliferation, fat accumulation, and autophagy in skin cells.

Hereinafter, embodiments of the present invention will be described in more detail with reference to the accompanying drawings. However, the following examples are only for embodiing the contents of the present invention, and the present invention is not limited thereto.

<Example 1> Preparation of gold and silver flower extract and fraction

Gold and silver coins used in the extraction of the present invention were commercially available dried gold and silver coins. For the extraction of gold and silver coins, gold and silver coins were added to the reactor and extraction was performed using ethanol, hexane, chloroform, and water as extraction solvents, respectively. The extraction solvent was used in an amount of 10 to 20 times the volume of the dried gold and silver coins, and extracted with stirring at room temperature for 24 hours. After extraction, filtration was performed using a reduced pressure filter, and the filtrate was removed from the solvent using a rotary vacuum concentrator to prepare an extract. The extract from which the solvent was removed was diluted with water and then fractionated in order of ethanol, hexane, and chloroform, and the solvent was removed with a rotary vacuum concentrator to prepare a fraction.

<Example 2> Cell proliferation test of extracts and fractions for each solvent of gold and silver flowers (cell proliferation test)

-Experiment method

After dispensing NIH3T3 cells into a 96-well cell culture plate at 5X10 ³ cells/well, extracts or fractions of each solvent were treated at different concentrations (0-100 μg/ml), and cell proliferation was measured after 72 hours.

-Experiment result

As a result of cell proliferation experiments using extracts of different solvents, it was confirmed that ethanol and hexane extracts had a cell proliferation effect (FIG. 2).

As a result of cell proliferation experiments using gold and silver fractions, it was found that all of the ethanol, hexane, chloroform, and water fractions had a cell proliferation effect, and in particular, it was confirmed that the cell proliferation effect was high in the ethanol extract (FIG. 3).

<Example 3> Lipid accumulation test of extracts and fractions for each solvent of gold and silver powder

-Experiment method

Dispense NIH3T3 cells into a 96-well cell culture plate at 5X10 ³ cells/well, treat extracts or fractions by solvent at different concentrations (0-100μg/ml), and accumulate lipids using AdipoRed reagent after 72 hours. Was measured.

-Experiment result

As a result of the fat accumulation experiment using each gold and silver extract, it was confirmed that the ethanol extract significantly improved the fat accumulation effect (FIG. 4).

From the results of the fat accumulation experiment using the gold and silver fraction, it was confirmed that the ethanol fraction had excellent lipid accumulation efficacy (FIG. 5).

In the cell proliferation results and fat accumulation results of Examples 2 and 3, it was confirmed that an active substance having a fat accumulation effect was present in the ethanol extract of gold and silver, and then, gold and silver extract using the ethanol extract of gold and silver. Wrinkle improvement and cell regeneration effect was confirmed.

<Example 4> Extract cell proliferation test (cell proliferation test) for each% of EtOH

-Experiment method

After dispensing NIH3T3 cells into a 96-well cell culture plate at 5X10 ³ cells/well, the extracts were treated with EtOH% extract at different concentrations (0-100 μg/ml), and cell proliferation was measured after 72 hours.

-Experiment result

As a result of the cell proliferation experiment, it was confirmed that the cell proliferation effect was obtained in 30, 50, and 70% ethanol extracts (FIG. 6).

<Example 5> Lipid accumulation test for each% of gold and silver EtOH

-Experiment method

Dispense NIH3T3 cells into a 96-well cell culture plate at 5X10 ³ cells/well, treat the extracts by% ethanol at different concentrations (0-100μg/ml), and measure the lipid accumulation using AdipoRed reagent after 72 hours. I did.

-Experiment result

As a result of the fat accumulation experiment, it was confirmed that the 70 and 100% ethanol extracts had excellent lipid accumulation efficacy (FIG. 7). Based on the cell proliferation results and fat accumulation results of Examples 4 and 5, a 70% ethanol extract of gold and silver flowers was selected, and subsequent experiments were conducted with a 70% ethanol extract.

<Example 6> Comparison of efficacy with insulin

Insulin is a factor that plays an important role in mammalian growth, fetal cell division and metabolic regulation. It also has an effect on cell proliferation and lipid accumulation in skin cells, and has an effect of inhibiting autophagy. It is known. In this example, the efficacy was compared by setting insulin as a positive control group.

-Experiment method

After dispensing NIH3T3 cells into a 96-well cell culture plate at 5X10 ³ cells/well, the extract and insulin (1μg/ml) by EtOH% were treated at different concentrations (0-100μg/ml), followed by cell proliferation and lipid accumulation after 72 hours. Was confirmed.

- Experiment result

As a positive control, insulin is effective in cell proliferation and fat accumulation, and the cell proliferation and fat accumulation efficacy was also confirmed in the 70% EtOH extract of gold and silver flowers (Fig. 8).

<Example 7> Efficacy of inhibiting autophagy of 70% EtOH extract

It was confirmed whether the extract of gold and silver flowers can inhibit autophagy that has progressed due to skin aging.

-Experiment method

1) Dispense NIH3T3 cells into a 96well cell culture plate at 5X10 ³ cells/well, and then extract and insulin (1μg/ml), 3-MA (1mM, inhibitor) by EtOH% by concentration (0-100μg/ml). After 2 hours of pretreatment, 500nM rapamycin (inducer) treatment to induce autophagy, and 72 hours later, the efficacy of autophagy inhibition was confirmed.

2) Dispense NIH3T3 cells at 1×10 ⁵ cells/well into a ^6- well cell culture plate, and induce autophagy after pretreatment of EtOH% extract and insulin (1 μg/ml) for each concentration (0-100 μg/ml) for 2 hours. For this, the efficacy of inhibiting autophagy was confirmed by Western blot after treatment with 500nM rapamycin and 72 hours.

-Experiment result

As a result of the experiment, it was confirmed that the extract of gold silver flower 70% EtOH has the effect of inhibiting autophagy (FIG. 9), and when the autophagy related marker LC3 was confirmed, the ratio of LC3-II and I decreased. It was confirmed that it was suppressed (Fig. 10).

<Example 8> Increased cell proliferation and fat accumulation when treated with 70% ethanol extract of gold silver flower in a state in which autophagy was induced

-Experiment method

Dispense NIH3T3 cells into a 96-well cell culture plate at 5X10 ³ cells/well, and add EtOH% extract, insulin (1μg/ml), and 3-MA (1mM, inhibitor) by concentration (0-100μg/ml). After 2 hours of pretreatment, 500 nM rapamycin (inducer) was treated to induce autophagy, and after 72 hours, cell proliferation and lipid accumulation were confirmed.

-Experiment result

As a result of the experiment, it was confirmed that cell proliferation and fat accumulation, which were reduced by induction of autophagy, increased again when treated with 70% EtOH extract of gold and silver flowers (FIG. 11).

<Example 9> MMP1, MMP3, collagen expression confirmation when treated with 70% EtOH extract

By confirming the expression of MMP1, MMP3, and collagen, which are markers related to skin elasticity, the efficacy of the 70% EtOH extract of Geumeunhwa 70% EtOH on skin cells and wrinkle improvement was confirmed.

-Experiment method

After dispensing NIH3T3 cells into 6 well cell culture plate at 5X10 ³ cells/well, damage is induced by TNF-α treatment, treated with extracts of EtOH% by concentration (25-100μg/ml), and harvested after 24 hours. After (cell harvest) treatment, collagen expression was confirmed using qRT-PCR, and MMP1 and MMP3 expression were confirmed using western blot.

- Experiment result

1) Check collagen expression

It was confirmed that collagen expression was increased in the group treated with the 70% EtOH extract of gold and silver compared to the TNF-α treatment group (FIG. 12).

2) MMP1, MMP3 expression confirmation

It was confirmed that the expression of MMP1 and MMP3 was decreased in the group treated with the 70% EtOH extract of gold and silver compared to the TNF-α treatment group (FIG. 13).

As described above, specific embodiments of the present invention have been described in detail, but those skilled in the art who understand the spirit of the present invention can add, change, delete other components within the scope of the same idea, However, other embodiments included within the scope of the inventive concept may be easily proposed. Therefore, it should be understood that the embodiments described above are illustrative in all respects and not limiting. The scope of the present invention is indicated by the scope of the claims to be described later rather than the detailed description described above, and all changes or modified forms derived from the meaning and scope of the claims and their equivalent concepts are included in the scope of the present invention. Should be interpreted as.

Claims (9)

In a cosmetic composition for increasing fat accumulation in skin cells, comprising an extract of Lonicera japonica and a fraction thereof as an active ingredient,
The extract and its fractions are 70% ethanol extracts and 70% ethanol fractions,
The gold and silver extract and its fractions are 0.01 to 20% by weight based on the total weight of the composition, a cosmetic composition for increasing fat accumulation in skin cells.
The method of claim 1,
The fractions are extracted from silica gel column chromatography, thin layer chromatography, or high performance liquid chromatography.
A cosmetic composition for increasing fat accumulation in skin cells, which is further purified using liquid chromatography).
delete delete delete delete The method of claim 1,
The cosmetic composition is a flexible lotion, gel, water-soluble liquid, milk lotion, nutrition cream, massage cream, essence, oil-in-water emulsion, water-in-oil emulsion, anhydrous face product, solid anhydrous product, oil in aqueous phase using globules Dispersion, ionic fat vesicle, nonionic fat vesicle, cleansing foam, cleansing water, pack, body oil, oil-in-water makeup base, water-in-oil makeup base, foundation, skin cover, lipstick, lip gloss, face powder, two-way A cosmetic composition for increasing fat accumulation in skin cells, characterized in that it is one formulation selected from the group consisting of cake, eye shadow, mascara, cheek color and eyebrow pencil.
in vitro,
A method of increasing fat accumulation in skin cells by treating gold and silver flower 70% ethanol extract. delete

Images