KR102169081B1 - Composition for stimulating hair growth and preventing depilation containing oregano oil as an active ingredient - Google Patents

Abstract

The present invention relates to a composition for promoting hair growth and hair restoration, and preventing hair loss containing an oregano oil as an active component. The composition for promoting hair growth and hair restoration, and preventing hair loss containing the oregano oil as the active component according to the present invention contains the oregano oil as the active component, wherein the oregano oil can be contained in a weight ratio of 0.01 to 5.0 wt% based on the total content of a cosmetic composition. By the configuration, the present invention soothes the scalp and relieves skin inflammation to improve scalp health, prevents hair loss by strengthening the hair roots, and also can exhibit excellent effects on promoting hair growth and hair restoration, and hair growth without side effects or toxicity to the human body.

Description

Composition for promoting wool and hair growth and preventing hair loss containing oregano oil as an active ingredient {COMPOSITION FOR STIMULATING HAIR GROWTH AND PREVENTING DEPILATION CONTAINING OREGANO OIL AS AN ACTIVE INGREDIENT}

The present invention relates to a composition for promoting wool and hair growth and preventing hair loss comprising oregano oil as an active ingredient, and more particularly, by preparing a cosmetic composition using a natural plant extract containing oregano oil as an active ingredient, As an active ingredient, oregano oil, which soothes skin inflammation, improves scalp health, and prevents hair loss by strengthening hair roots, promotes wool and hair growth, and has excellent effects on hair growth without side effects or toxicity to the human body. It relates to a composition for promoting hair growth and preventing hair loss.

In general, hair has a function of absorbing and discharging heavy metals such as arsenic, mercury, and zinc, which are unnecessary to the body, while protecting the human body from external stimuli such as buffering action, UV protection, cold cooling, and friction from external impacts. Currently, not only these basic functions, but also cosmetic functions that create new images and create one's own personality and confidence are becoming more important.

This hair growth is usually carried out by repeating a series of cycles such as the growth phase, the degenerative phase, and the resting phase. In addition, the causes of hair loss are known to be caused by a variety of causes, such as genetics and diseases, hormonal changes, trauma and stress, malnutrition and menopausal disorders, and abuse of chemicals and certain drugs.

In recent years, as social activities increase not only among young men, but also among women, the interest in hair loss prevention, wool, and hair growth is increasing, and this interest is expected to increase as social activities continue to increase and social and cultural factors change. I think.

The types of hair growth agents can be broadly divided into those that promote blood circulation or supply nutrients to the hair, those that promote metabolism, reduce fat content, or inhibit reductases, but they do not all show a great effect. It is known.

Until now, the only drugs approved by the Food and Drug Administration (FDA) as hair regrowth agents are minoxidil and Propecia. Minoxidil was originally developed as a hair growth agent, which was confirmed to promote hair growth to patients who used this drug as a blood pressure enhancing agent.However, its effect is insufficient on its own, so the desired effect can be obtained only after applying a skin irritant such as tretinoin. In addition, Propecia developed by MSD is an oral treatment for hair loss that has the effect of preventing and treating male pattern hair loss by inhibiting the production of DHT, which inhibits the action of 5-alpha-reductase. There are reports that side effects of

"Minoxidil", which has been used as a conventional hair growth stimulator, was originally used as a treatment for hypertension that relaxes blood vessels, but it has side effects of growing up to 2 to 4 cm of hair all over the body such as head, arms and legs, and several months when the drug is stopped. It started to be used as a hair growth accelerator applied by focusing on the hair falling out of the inside. Minoxidil is generally used for male hormonal alopecia, and its effect is certified for parietal alopecia, but has little effect on temporal alopecia. In addition, excessive use may cause hypotension or cardiovascular complications.

In addition, "Propecia", which uses an anti-androgen drug that inhibits the production, binding, and metabolism of male hormone androgens, is a 5-alpha-li that blocks the conversion of testosterone to dihydrotestosterone (DHT). As a 5-alpha reductase enzyme inhibitor, it is the same drug as Proscar, which has been used to treat prostatic hypertrophy, and has been reported to cause specific external genital anomalies in the fetus when administered orally to pregnant monkeys. When these drugs are used in men, they can cause feminization, and even in women during pregnancy, they may cause feminization of the male fetus and cause congenital malformations.

However, when the above-described hair loss prevention or hair growth agent is applied, various side effects are often observed due to an imbalance of body homeostasis, and various unexpected side effects such as decreased sexual function, allergy, depression, and local inflammation occur.

In addition, since hair loss occurs due to a complex system, there is a problem that narrow treatment such as hormone suppression is only a myopic prescription.

Domestic registered patent No. 10-1263181 (registered on May 06, 2013) Domestic registered patent No. 10-1645640 (registered on July 29, 2016) Domestic registered patent No. 10-1141779 (registered on April 24, 2012)

The present invention prepares a cosmetic composition using a natural plant extract containing oregano oil as an active ingredient, so as to soothe the scalp and alleviate skin inflammation to improve scalp health, and to prevent hair loss by strengthening hair roots, and It is intended to provide a composition for promoting wool and hair growth and preventing hair loss, including oregano oil, which has no side effects or toxicity, and has an excellent effect on hair growth and hair growth, as an active ingredient.

Various problems to be solved by the present invention are not limited to the problems mentioned above, and other problems that are not mentioned will be clearly understood by those skilled in the art from the following description.

The composition for promoting wool and hair growth and preventing hair loss comprising oregano oil according to the present invention as an active ingredient contains oregano oil as an active ingredient, wherein the oregano oil is in a weight ratio of 0.01 to 5.0% by weight based on the total content of the cosmetic composition. Can be included.

In addition to the oregano oil, avocado oil, Amla oil, bhringraj oil, green tea oil, Eoseongcho extract, and a natural plant extract consisting of puer tea extract are further included, the oregano oil The natural plant extract may be included in a weight ratio of 50 to 150 parts by weight based on 100 parts by weight.

The natural plant extract is 3 to 7 parts by weight of avocado oil, 5 to 10 parts by weight of Amla oil, 1 to 3 parts by weight of bhringraj oil, 4 to 8 parts by weight of green tea oil It may be included in a weight ratio of 10 to 20 parts by weight of the part, Eoseongcho extract, and 8 to 12 parts by weight of the puer tea extract.

In addition to the oregano oil and natural plant extract, hyaluronic acid, zinc gluconate, magnesium gluconate, and jade powder are further included, but hyaluronic acid 1 to 100 parts by weight of the oregano oil and natural plant extract It may be included in a weight ratio of 3 parts by weight, 2 to 4 parts by weight of zinc gluconate, 3 to 6 parts by weight of magnesium gluconate, and 7 to 13 parts by weight of jade powder.

Details of other embodiments are included in the detailed description.

The composition for promoting wool and hair growth and preventing hair loss comprising oregano oil according to the present invention as an active ingredient soothes the scalp, relieves skin inflammation, improves scalp health, and can prevent hair loss through strengthening the hair roots. There is no side effect or toxicity, and it can show excellent effects on hair growth and promotion of wool and hair growth.

It will be fully understood that the embodiments of the technical idea of the present invention can provide various effects not specifically mentioned.

1A is a photograph showing the results of an antimicrobial test in which oregano oil was applied to Staphylococcus aureus, a strain used.
1B is a photograph showing the results of an antimicrobial test without oregano oil.
Figures 2a to 5b are hair growth before (2a, 3a, 4a, 5a) and after 8 months (2b, 3b, 4b, 5b) using the mixture of oregano oil and natural plant extract according to the present invention This is a picture showing the status.

Advantages and features of the present invention, and a method of achieving them will become apparent with reference to the embodiments described below in detail. However, the present invention is not limited to the embodiments described herein and may be embodied in other forms. Rather, the embodiments introduced herein are provided so that the disclosed content may be thorough and complete, and the spirit of the present invention may be sufficiently conveyed to those skilled in the art.

The terms used in the present application are only used to describe specific embodiments, and are not intended to limit the present invention. Singular expressions include plural expressions unless the context clearly indicates otherwise.

Unless otherwise defined, all terms including technical or scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which the present invention belongs. Terms such as those defined in a commonly used dictionary should be interpreted as having a meaning consistent with the meaning of the related technology, and should not be interpreted as an ideal or excessively formal meaning unless explicitly defined in this application. Does not.

Hereinafter, a preferred embodiment will be described in detail with respect to a composition for promoting hair growth and preventing hair loss and wool comprising oregano oil according to the present invention as an active ingredient.

The composition for promoting wool and hair growth and preventing hair loss according to the present invention contains oregano oil as an active ingredient.

In addition, the composition for promoting wool and hair growth and preventing hair loss according to the present invention includes avocado oil, Amla oil, bhringraj oil, green tea oil, Eoseongcho extract in addition to the oregano oil. And it may further include a natural plant extract consisting of puer tea extract.

In addition, in the composition for promoting wool and hair growth and preventing hair loss according to the present invention, the oregano oil may be included in a weight ratio of 0.01 to 5.0% by weight based on the total content of the cosmetic composition.

In addition, the composition for promoting wool and hair growth and preventing hair loss according to the present invention may further include the natural plant extract in a weight ratio of 50 to 150 parts by weight based on 100 parts by weight of the oregano oil, the natural plant extract is avocado ( Avocado) 3 to 7 parts by weight of oil, 5 to 10 parts by weight of Amla oil, 1 to 3 parts by weight of bhringraj oil, 4 to 8 parts by weight of green tea oil, Eoseongcho extract 10 to 20 It may be included in a weight ratio of 8 to 12 parts by weight and a puer tea extract.

In addition, the composition for promoting wool and hair growth and preventing hair loss according to the present invention may further include hyaluronic acid, zinc gluconate, magnesium gluconate, and ox powder in addition to the oregano oil and natural plant extracts. There may also be, 1 to 3 parts by weight of hyaluronic acid, 2 to 4 parts by weight of zinc gluconate, 3 to 6 parts by weight of magnesium gluconate, and jade based on 100 parts by weight of the oregano oil and natural plant extract. It may be included in a weight ratio of 7 to 13 parts by weight of powder.

In addition, the composition for promoting wool and hair growth and preventing hair loss according to the present invention is from the group consisting of lotion, shampoo, emulsion, cream, essence, cosmetic ointment, spray, gel, pack, sun cream, makeup base, foundation, powder, and detergent. It may be included in any one or more formulations selected.

In addition, the composition for promoting wool and hair growth and preventing hair loss according to the present invention may be included in any one or more formulations selected from the group consisting of ointments, patches, gels, creams, and sprays.

The oregano oil refers to oil extracted from oregano (Oregano or Origanum vulgare), and the oregano (Oregano or Origanum vulgare) is originated from the Mediterranean coast, and was used importantly for medicinal purposes in ancient Greece, and was used for cooking in ancient Rome. Even now, it is used as a natural preservative for meat storage and as a spice in Italian cuisine, so it is beneficial beyond harmless to the human body.

Oregano, also known as a natural antibiotic, has been said to be effective in tonic, diuretic, dry stomach, appetite, soothing, sterilizing, headache, motion sickness, and insomnia since ancient Greek times, so it is mainly boiled as a tea or used as a bath. helpful.

For example, as the oregano oil used in the present invention, a commercially known oregano oil may be used, or oregano oil prepared by the following manufacturing method may be used.

First, oregano stems and raw oregano leaves can be separated and prepared, and then washed.

Next, the washed oregano stem may be dried. In the drying, the washed oregano stem may be dried for 4 to 6 days at a temperature of 23 to 27°C.

Thereafter, the dried oregano stem may be cut in units of a predetermined length, for example, the dried oregano stem may be cut in units of 0.5 to 2.0 cm in length.

Subsequently, the cut oregano stems may be mixed with lactic acid bacteria and then aged. The aging is accomplished by maintaining the oregano stems mixed with the lactic acid bacteria at a temperature of 28 to 32°C and a humidity of 50 to 55% for 20 to 30 hours. I can make it.

For example, as the lactic acid bacteria, any one known lactic acid bacteria selected from the group consisting of lactic acid bacteria ( Lactobacillales ), Streptococcus thermophiles , and Bifidobacterium longum may be used.

Next, the washed oregano leaves can be heated with water vapor, the heating is performed by heating the washed oregano leaves at a temperature of 125 to 135°C and a pressure of 2 to 3kgf/cm ² with water vapor for 10 to 30 minutes Can be.

Next, the fresh oregano leaves heated with water vapor may be dried, wherein the drying is performed by drying the oregano leaves at a temperature of 20 to 25° C. in order to prevent the chlorophyll of the fresh oregano leaves from being destroyed and volatilization of useful components when drying in sunlight. It can be dried for 20 to 40 hours at.

Subsequently, the dried oregano leaves may be heated to roast the dried oregano leaves in a heating container (eg, cauldron) for 3 to 5 minutes at a temperature of 70 to 80°C. After the first roasting, the first roasted oregano leaves may be secondarily roasted at a temperature of 110 to 120°C for 20 to 50 seconds.

Next, the raw oregano leaves may be fermented by spraying a Bacillus culture solution on the roasted oregano leaves.

The Bacillus bacillus culture solution is capable of promoting fermentation of the dried oregano leaves, sprayed at a weight ratio of 10 to 20 parts by weight of Bacillus bacillus culture solution to 100 parts by weight of the roasted oregano leaves, and a temperature of 50 to 60°C and 60 to 65 After allowing the% humidity to be maintained, it can be fermented for 2-4 days.

The Bacillus Bacillus culture medium is produced by mixing Bacillus bacillus and water, and the Bacillus bacillus is grown twice a day at 25°C after mixing 10 to 12% by weight of brown sugar in godubap, placing eco-friendly rice husks on the prepared medium and covering a cloth. Bacillus bacteria are produced by incubating for 1 week while spraying water on them, and they can be re-inoculated in a medium mixed with rice bran and brown sugar at a weight ratio of 50:50 and cultured at 25° C. for 1 week to be used as the Bacillus bacteria culture solution used in the present invention. have.

In the present invention, the Bacillus subtilis ( Bacillus subtilis ), Bacillus subtilis spjijepiyi ( Bacillus subtilis ), Bacillus megaterium ( Bacillus megaterium ), Bacillus licheniformis ( Bacillus licheniformis ) and Bacillus fulmis Any one or more selected from the group consisting of ( Bacillus pumilus ) may be used.

Next, the fermented oregano stem and the fermented oregano raw leaves are mixed and fermented to prepare a fermented oregano mixture.

In the step, after mixing in a weight ratio of 300 to 500 parts by weight of the fermented fresh oregano leaves with respect to 100 parts by weight of the matured oregano stems, fermentation for 3 to 5 days at a temperature of 60 to 65 °C and a humidity of 55 to 60% To prepare a fermented oregano mixture.

Subsequently, the oregano fermentation broth may be prepared by mixing a solvent with the fermented oregano mixture and applying ultrasonic waves.

In order to prepare the oregano fermentation broth, the fermented oregano mixture is mixed with a solvent to prepare a mixed solution, and then added to an ultrasonic extractor to apply ultrasonic waves to the mixed solution to prepare an oregano fermentation broth, the solvent being the fermented oregano mixture 100 It is contained in a weight ratio of 300 to 500 parts by weight, and ethanol may be used as the solvent.

In addition, the ultrasonic wave applied to the mixed solution in order to prepare the oregano fermentation broth may use an output of 100 to 200 watts for 50 to 100 minutes at a vibration frequency of 60 to 80 KHz.

Next, solids such as oregano stems and fresh oregano leaves may be removed from the oregano fermentation broth, and the separation of solids such as oregano stems and fresh oregano leaves may be performed using a known strainer such as a sieve.

Next, the oregano fermentation broth from which the solid content has been removed may be centrifuged.

In the above step, by centrifuging the oregano fermentation broth from which the solid content has been removed, the oregano stems and the fine particles of the green leaves are located, and the intermediate layer and the supernatant are located above the lower layer. .

Next, oregano oil containing useful components of oregano may be prepared by extracting the fermented oregano broth located in the intermediate and supernatant.

In the above step, the oregano oil is first extracted with 10 to 15 ℓ of a 70% (v/v) ethanol aqueous solution in 2000 to 3000 g of oregano fermentation broth for 3 to 4 hours at 85 to 90°C, and then 70% (v/v) aqueous ethanol solution Completing the secondary extraction at 85 to 90°C for 2 to 3 hours at 4 to 6ℓ, and preparing an extract until it becomes 1000 to 1500 ㎖ in a vacuum rotary evaporator; And ethanol is added to the extract, diluted to 2 to 3 times by weight, centrifuged at 18,000 to 20,000 rpm for 30 to 40 minutes to completely remove polymer fibers and solids that may be included in the oregano fermentation broth, and then the collected supernatant is 1.5 to It can be prepared by extracting twice with 2.0 times the weight of acetic acid.

The avocado oil can be extracted from avocado, and the avocado oil contains a large amount of unsaturated fatty acids, which greatly reduces the incidence of heart disease, cancer, and diabetes, and also increases the absorption rate of beta-carotene when consumed with green and yellow vegetables. It can be increased by 15.3 times.

The avocado is rich in vitamins and minerals, effectively decomposes cholesterol, can prevent various adult diseases and vascular diseases, is good for a short-term diet, and is rich in lutein and zeagentin, so it is good for eye health.

In addition, the avocado contains important antioxidants such as vitamin C and vitamin E, so it can fight inflammation and alleviate the symptoms of arthritis, and oleic acid, an unsaturated fatty acid of avocado, is used for cholesterol and fat in cells. Can prevent the synthesis of

For example, as the avocado oil, avocado oil prepared by the following manufacturing method may be used.

First, after preparing the avocado, you can wash it thoroughly in water.

Next, the washed avocado may be immersed in grape juice and a rancidity inhibitor. It is possible to prevent discoloration or rancidity of the avocado oil prepared by immersing the avocado in grape juice and rancidity inhibitor as described above.

The washed avocado can be immersed in a mixture of grape juice and a rancidity inhibitor in a ratio of 1:1 by weight, and the rancidity inhibitor is mixed with vitamin C and a salt solution in a weight ratio of 1:1. Can be manufactured.

The grape juice is good for preventing blood clots and preventing adult diseases such as high blood pressure, arteriosclerosis and heart disease, and can help improve memory, and the salt solution used as the rancidity inhibitor may be a salt solution having a concentration of 1 to 2% by weight, After immersing in a weight ratio of 100 to 120 parts by weight of a mixture of grape juice and rancidity inhibitor with respect to 100 parts by weight of the washed avocado, it may be maintained for 10 to 40 minutes.

Thereafter, the avocado immersed in the mixture of grape juice and rancidity inhibitor may be taken out so that the grape juice and rancidity inhibitor components penetrate into the avocado.

In the above step, by allowing the active ingredient of grape juice and rancidity inhibitor to penetrate to the surface of the avocado, it is possible to prevent discoloration or rancidity of the avocado oil produced even after a long period of time.In this step, in the mixed solution of grape juice and rancidity inhibitor It can be carried out by storing the separated avocados at a temperature of 28 to 32° C. for 60 to 100 minutes.

Subsequently, the avocado into which the components of the grape juice and rancidity inhibitors have penetrated may be steamed.

The steaming of the avocado can be performed by steaming the avocado into which the components of the grape juice and rancidity inhibitors are infiltrated with water vapor for 50 to 100 minutes.If the steaming of the avocado is performed below the above lower limit, sufficient steaming is performed. There is a problem that is not achieved, and when the above upper limit range is exceeded, the difference in effect according to the increase of the steaming time may be insignificant.

Next, the steamed avocado can be dried.

Drying of the steamed avocado may be performed for 10 to 20 hours at a temperature of 45 to 50°C of the steamed avocado, and mechanical drying using a dryer may be used in order to keep the drying temperature of the avocado constant. have.

Next, the dried avocado can be washed with one or more acids of acetic acid or citric acid, and the washing of the dried avocado is by washing the surface of the dried avocado with at least one acid of acetic acid or citric acid. There is an effect of improving the flavor and color of avocado oil to be manufactured through a fermentation process.

Subsequently, a fermentation broth composed of a culture broth and lactic acid bacteria may be sprayed on the washed avocado.

As the lactic acid bacteria, any one known lactic acid bacteria selected from the group consisting of lactic acid bacteria ( Lactobacillales ), Streptococcus thermophiles , and Bifidobacterium longum may be used, and the culture medium is peptone , Malt extract, sodium citrate, potassium phosphate dibasic, glucose, and distilled water, and the culture solution is 15 to 25 parts by weight of peptone, malt extract 5 to 15 It may be made by mixing in a weight ratio of 0.5 to 1.5 parts by weight of sodium citrate, 0.1 to 0.3 parts by weight of dibasic potassium phosphate, 1 to 3 parts by weight of glucose, and 100 to 200 parts by weight of distilled water.

In the culture medium, the peptone is a nitrogen source that supplies protein and supplies proteins necessary for the growth of microorganisms, and the malt extract contains various minerals, vitamin B groups, and amino acids, so that it can help the growth of microorganisms, and the sodium citrate Silver is an inorganic substance that maintains a state optimized for the growth of microorganisms by adjusting the pH of the culture medium, and can supply nutrients, and the glucose can function as an important component by which lactic acid bacteria metabolize sugar to produce organic acids.

Next, the avocado sprayed with the fermentation broth can be fermented, and the fermentation of the avocado is 3 to 6 days in a storage container maintaining a temperature of 45 to 50°C and a humidity of 60 to 65% of the avocado sprayed with the fermentation broth. Can be stored for fermentation.

Then, the fermented avocado may be dried in cold air.

Cold air drying of the fermented avocado may be performed by drying the fermented avocado with cold air at a temperature of 3 to 6°C for 5 to 10 hours, and maintaining the fermented avocado at a temperature of 55 to 60°C for 1 to 3 days.

Subsequently, the cold-air dried avocado is compressed at a humidity of 70 to 80%, a temperature of 120 to 150°C, and 3 to 5kgf/cm ² , and then the oil is separated, thereby preparing avocado oil.

As the amra oil, a known oil extracted from amra may be used, and the amra is generally known as an Indian gooseberry and a deciduous tree. It is widely distributed in tropical and subtropical countries such as China, India, Indonesia, and Taiwan, and is known to contain vitamin C, minerals, amino acids, tannins, filamblic acid, rutin, curcuminoids, emblemols, and phenolic compounds. (Zhang YJ et al., J Nat Prod, 63: 1507-1510, 2000). Amra contains 20 times more vitamin C than oranges, and it is very stable to heat, so even when exposed to high temperatures for a long time, the vitamins are hardly destroyed like when freshly harvested from trees. It is believed that the vitamin C durability and heat resistance of Amra originate from the tannins contained together. The antioxidant, anti-tumor, and anti-inflammatory activities of Amra fruit extract are known to be influenced by the high content of vitamin C and polyphenols.

The Bringgraz oil has been used as a medicinal material that enriches hair for centuries in India, and the Bringgraz is also called nasturtium, and the eclipta prostrate is an annual herb of the chrysanthemum family with a height of 10 to 70 cm. It is a plant that occurs a lot in soybean fields in Jeju Island, southern and central regions of Korea. From ancient times, it has been known to be effective in promoting blood flow, pain relief, immunity, dermatitis, promoting hair growth and dermatitis where the skin is cracked. In particular, it is known to be effective in relieving skin irritation.

The green tea oil may be extracted from the seeds of the green tea tree (Camellia sinensis), and 30-40% of the polyphenol components found in green tea are known as flavonols and catechins, and the main component of green tea catechins is epicatechin. , Epicatechin-3-gallate (epcicatechin-3-gallate), epigallocatechin (epigallocatechin), epigallocatechin-3-gallate (epigallocatechin-3-gallate; EGCG), among which epigallocatechin- It is known that the content of the 3-gallate component is the highest, and the polyphenol component of green tea is known to have various efficacy effects such as antioxidant, anticancer, anti-inflammatory, and antibacterial.

For example, the green tea oil is (a) first extracted with 15 ℓ of 75% (v/v) ethanol aqueous solution to 1.5 kg of crushed green tea tree seeds at 80 to 85°C for 4 hours, and then under the same conditions with 8 ℓ of the same solvent. Completing the secondary extraction under the vacuum rotary evaporator until 1200 to 1400 ㎖ to prepare a concentrated solution; (b) adding water to the concentrate, diluting it 2 to 3 times by weight, centrifuging at 14,000 to 15,000 rpm for 30 to 40 minutes to completely remove solid components, and then extracting the collected supernatant twice with 1.5 times of chloroform; And (c) removing the chloroform layer and extracting the aqueous layer with 1.5 times ethyl acetate five times to obtain green tea oil.

The Eoseongcho extract is prepared by extracting Eoseongcho, and Eoseongcho (Houttuynia cordata thunb) is an outpost of Yakmomil, a plant of the Saururaceae, and lives in Southeast Asia and particularly Japan and Korea. Eoseongcho is also called Sip-Yak because it has ten medicinal effects, and the stem is like a sweet potato leaf, and it is also called Eoseongcho because it originates from the fact that when you touch the raw leaves, fish smells badly. Eoseongcho is also available for medicinal and edible use. It is classified as animals and plants that can only be used in a minimum amount as an auxiliary ingredient in the classification of food raw materials in the food code. Pharmacologically, it is widely known for its strong heart, diuretic, antibacterial, detoxifying, and anticancer effects. Is used as a cosmetic and health functional food that helps detoxification and beauty.

Eoseongcho extract prepared by the following manufacturing method may be used as the extract of Eoseongcho.

First, in order to prepare an extract of Eoseongcho, it may be washed after preparing Eoseongcho.

Next, the washed Eoseongcho may be pulverized into a certain particle size.

In the above step, the active ingredient of the Eoseongcho may be sufficiently extracted by pulverizing the Eoseongcho to a certain particle size, and the crushing of the Eoseongcho may be pulverized so that the particle diameter is in the range of 500 to 2500㎛. In the above step, if the particle diameter of the Eoseongcho is pulverized to less than 500㎛, it takes a long time to pulverize, and the particles are too small to cause agglomeration between particles in a later process. It may be difficult to effectively extract useful ingredients from Eoseongcho.

Thereafter, the pulverized Eoseongcho may be extracted by applying ultrasonic waves in an atmosphere of low temperature and reduced pressure to prepare a mixed solution for extracting Eoseongcho.

In the above step, after preparing a mixed solution by mixing the pulverized Eoseongcho with a solvent, it is added to an ultrasonic extractor to apply ultrasonic waves to the mixed solution to prepare a Eoseongcho extract mixture, wherein the solvent is 100 parts by weight of the pulverized Eoseongcho It is mixed at a weight ratio of 1000 to 2000 parts by weight, and any one or more solvents selected from water or an alcohol having 1 to 4 carbon atoms (C1 to C4) may be used as the solvent.

In addition, in the step, the ultrasonic wave applied to the mixed solution under a temperature of 20 to 25°C and a pressure of 0.1 to 0.5kgf/cm ² outputs 100 to 200 watts for 30 to 60 minutes at a vibration frequency of 30 to 50 KHz. It can be extracted using

Subsequently, the Eoseongcho extract can be prepared by removing solids such as pulverized Eoseongcho from the Eoseongcho extract mixture and then aging for 1 to 3 hours at a temperature of 10 to 20°C.

In the above step, the removal of solids such as crushed Eoseongcho from the Eoseongcho extract mixture may be performed by separating and removing the solids using a known sieve such as a sieve.

The puer tea extract is prepared using puer tea, and the puer tea is black tea that is naturally fermented in a state of high pressure or mountain tea, processed with Camellia sinensis LINNE in Yunnan, China. It is a series of post-fermentation tea.

In Chinese literature, Bonchogangmoksiyu (本草綱目拾遺), puer tea has a unique scent, helps relieve hangovers and digestion, clears sputum, clears the stomach, relieves thirst, and removes harmful fat from the body. . In addition, since puer tea has no side effects even if taken for a long time, it is called beauty tea, diet tea, and longevity tea in Japan, France, Germany, Italy, and Macau (Son et al, Food & Nutr 18:219-224, 2005). ).

On the other hand, according to studies using puer tea, when alcohol was induced in a subacute state in ICR mice, the blood alcohol concentration of the puer tea extract intake group was not significantly different from that of the normal control group (Park et al, J East Asian Soc dietary Life, 14:634-639, 2004), puer tea and puer tea extracted from the results of examining the antioxidant effects of puer tea and puer tea proved that the total polyphenol content and electron donating ability were similar. (Son et al, Korea J Food & Nutr, 18:219-224, 2005).

In order to prepare the puer tea extract, first, the fresh puer tea leaves are prepared and washed to remove foreign substances attached to the green puer tea leaves, and then the washed green puer tea leaves may be dried.

In the present invention, in order to prevent the chlorophyll of the fresh puer tea leaves from being destroyed and the useful components volatilized during sunlight drying, the green puer tea leaves are dried for 5 to 10 hours at a temperature of 25 to 30°C. I can.

Next, the dried raw puer tea leaves may be heated to dryness.

In the step, the dried raw puer tea leaves are put into a heating container (for example, a cauldron), and then first roasted for 3 to 5 minutes at a temperature of 90 to 100°C, and the first roasted puer tea leaves are 130 It may proceed to the step of the second abrasion for 30 to 60 seconds at a temperature of to 140 ℃.

Next, a mixture of puer tea can be prepared by mixing a solvent with the roasted puer tea leaves and applying ultrasonic waves.

In the above step, after mixing the roasted puer tea leaves and a solvent, it is added to an ultrasonic extractor to apply ultrasonic waves to the mixture consisting of the roasted puer tea leaves and a solvent to prepare a puer tea mixture, wherein the solvent is the It is mixed in a weight ratio of 300 to 600 parts by weight based on 100 parts by weight of fresh puer tea leaves, and water may be used as the solvent.

In addition, the ultrasonic wave applied to the mixture consisting of the roasted puer tea leaves and the solvent may be extracted using an output of 300 to 400 watts for 30 to 60 minutes at a vibration frequency of 60 to 80 KHz.

Subsequently, the puer tea extract may be prepared by separating and removing solids such as green puer tea leaves from the puer tea mixture and then aging them.

In the above step, a known sieve such as a sieve may be used to separate the solids such as the fresh puer tea leaves, and the aging is performed by using the puer tea extract from which the solids are removed at a temperature of 10 to 15°C. This can be done by storing for hours.

The hyaluronic acid is mainly found in some types of tumors such as joint fluid or eye vitreous fluid of animals, connective tissues such as umbilical cord, dermal superficial layer, etc., and several types including A-type and C-type lytic streptococcus. It is composed of capsular components in bacteria of. In animal tissues, for example, non-cartilage of cattle, large chondroitin sulfate proteoglycans or binding proteins are combined to form large molecular aggregates. There are many very long sugar chains with a molecular weight of 105 to 106, but it varies depending on the material, and even the same material is never uniform. It has the property of making a gel by combining with a large amount of water, and this is linked to functions in the body such as lubrication of joints and flexibility of the skin.

In addition, when connective tissue is damaged, the synthesis of hyaluronic acid may be temporarily active for rapid recovery, and it is believed that it forms part of cellular activity in tissue reconstruction. CD44 was found as a hyaluronic acid-binding protein on the cell surface. Enzymes that hydrolyze or cleave glucosamine or glucuronide bonds of hyaluronic acid from animal tissues such as testis, skin, liver, snake venom, leeches, bacteria, and actinomycetes, respectively, have been discovered. In addition, when the connective tissue is damaged, the synthesis of hyaluronic acid may become active temporarily for rapid recovery, and it is believed that it forms a part of cellular activity in tissue reconstruction.

The zinc gluconate is used as a skin conditioning agent and is effective for inflammatory acne due to its antiviral properties. In addition, the zinc gluconate is effective in soothing the irritated scalp and relieving skin itching.

The magnesium gluconate is a magnesium salt component of gluconic acid, the chemical formula is C ₁₂ H ₂₂ MgO ₁₄ or C ₁₂ H ₂₂ MgO ₁₄ 2H ₂ O, the molecular weight is 414.61, and the skin condition. It helps to keep it in a healthy and good condition.

The jade powder functions to improve the moisturizing effect by making the skin smooth and fine, and effectively delivering nutrients to the skin. Jade is divided into jadeite and purgatory, and jadeite is a lead pyroxene mineral belonging to the pyroxene group, and is a monoclinic material composed of silicic acid, aluminum oxide, and soda. The hardness is a dense mass like crystal, and the light is black, cyan, green, transparent or translucent, and jade is usually referred to as jadeite. Purgatory is a monoclinic fibrous mineral body of inosilicate, and is divided into purgatory and serpentine super-basic purgatory among high-soil marbles. The degree is determined, and it is known that the finer the fiber, the better the quality.

Hereinafter, preferred examples and comparative examples of a composition for promoting hair growth and preventing hair loss and wool comprising oregano oil according to the present invention as an active ingredient will be described in more detail with reference to the accompanying drawings.

A shampoo was prepared using the cosmetic compositions according to Examples 1, 2, and 3 and Comparative Examples so that the ingredients as shown in the following [Table 1] were included.

Ingredient (% by weight) Example 1 Example 2 Example 3 Comparative example Oregano oil 0.1 2 0.05 - Natural plant extract 0.13 2.3 0.05 2 Cocobetain 15 15 15 15 Poly Quarter One One One 2 LES (disodium lauret sulfosuccinate) 45 50 45 45 glycerin 3 2 5 4 Olive Sun Care Wax 8 5 7 10 Emulsifying Wax 3 4 5 5 Spices 0.1 0.1 0.1 0.1 Distilled water All remaining All remaining All remaining All remaining

Here, the natural plant extract is avocado oil, Amla oil, bhringraj oil, green tea oil, Eoseongcho extract and puer tea extract are 5:8:2:6:15: It was to be included in a weight ratio of 10.

1. Stability test

Stability tests of shampoos prepared according to Examples 1, 2 and 3 were performed.

The observation period was the same for one week, and stability was observed at various temperatures, and the results are shown in [Table 2].

Temperature Example 1 Example 2 Example 3 0℃ Good Good Good 40℃ Good Good Good -20℃ Good Good Good

Referring to [Table 2], it was confirmed that the shampoos prepared according to Examples 1, 2, and 3 had good formulation stability.

2. Hair conditioning effect test (sensory evaluation)

Using the sampu prepared according to Examples 1, 2, 3 and Comparative Examples, 5 points (very bad, bad, normal, and after having been used for one week to a judge consisting of 20 people aged 20 to 40 years) Good, very good) The quality was evaluated through a questionnaire and the results are shown in [Table 3].

Survey item Example Comparative example One 2 3 Foamy and soft 4.6 4.5 4.5 3.1 Clean rinse 4.4 4.2 4.5 3.2 Easy to comb when semi-dry 4.5 4.4 4.3 3.3 The hair is soft after drying 4.6 4.3 4.1 3.5 The hair is moist after drying 4.5 4.6 4.3 3.1 Glossiness is felt on the hair after drying 4.7 4.5 4.4 3.5

3. Scalp irritation test

The stimulation clinical test for the shampoo prepared as in Example 1 was conducted as follows.

First, the clinical trial was conducted on 20 healthy subjects (8 males and 12 females) between the ages of 10 and 50, and it was confirmed that no other products such as steroids or antibiotics were used from 2 weeks before the start of the experiment to the end of the experiment. .

And for all the test subjects, the scalp condition was observed 24 hours after shampooing, and a qualitative evaluation was performed through the evaluation table as shown in [Table 4].

Experiment item Subjective score Evaluation results

Degree of stimulation a: The skin turns red and the itching is strong - b: The skin is slightly red and itchy - c: The skin is slightly red and irritated. - d: There is no change in skin condition, but it is slightly irritated 2 people e: No irritation or change in skin condition 18 people

Referring to [Table 4], it can be seen that the shampoo prepared according to Example 1 can be safely used because there is no scalp irritation or change in skin condition.

4. Antibacterial effect-1

In order to confirm the antibacterial effect of the cosmetic composition according to Examples 1, 2, 3 and Comparative Examples, the test of the microbiology guidelines of The Cosmetic, Toility and Fragrance Association, Inc. (CTFA) Method was used. In addition, the bacteria used to confirm the antibacterial effect include Gram-positive bacteria as Staphylococcus aureus ATCC 6538P, Gram-negative bacteria as Escherichia coli (Escherichia coli ATCC 8739), Pseudomonas aeruginosa ATCC 9027), and yeast as Candida albicans. (Candida albicans ATCC 10231), Aspergillus niger ATCC22343 was used.

A suspension of 3 bacteria (Ecoli, P aeruginosa, S aureus) and 2 fungi (Calbicans, A niger) was mixed in a vial containing 10 g of each cosmetic composition, respectively, 1 × 106 cfu/g, 1 × 105 After inoculation so that it becomes cfu/g, 1g of sample is collected under aseptic operation at intervals of 4 hours, 1 day, 2 days, 3 days, 7 days after storage at 25℃, diluted appropriately with a diluent, and bacteria are nutrient agar ( Nutrient agar, Difco), and fungi were spread on potatodextrose agar (Difoc) medium and incubated at 35°C and 25°C for 24 to 72 hours, respectively, and then the D-value was obtained through the number of bacteria.

ingredient Fungus D-value(Hr) Example 1 Germ 5.1 Fungi 5.0 Example 2 Germ 5.2 Fungi 5.2 Example 3 Germ 5.1 Fungi 5.2 Comparative example Germ 8.5 Fungi 8.8

* D-value: Time required for 90% of microorganisms to die

Referring to [Table 5], it was confirmed that the cosmetic compositions according to Examples 1, 2, and 3 had very excellent antibacterial effects.

5. Antibacterial effect-2

The antimicrobial properties of oregano oil contained in Examples 1, 2 and 3 were tested.

The experiment for antimicrobial activity was performed on Staphylococcus aureus.

1A is a photograph showing the results of an antimicrobial test in which oregano oil was applied to Staphylococcus aureus, a strain used, and FIG. 1B is a photograph showing the results of an antimicrobial test without oregano oil.

Referring to FIGS. 1A and 1B, in an experiment using oregano oil, it was confirmed that Staphylococcus aureus, a strain used, showed 99.9% antibacterial activity.

6. Hair growth test

After mixing the oregano oil and natural plant extracts used in Examples 1, 2 and 3 in a weight ratio of 1:1, shampooing the hair of the subject was repeated twice a day in the morning and evening.

As described above, a mixture of oregano oil and natural plant extract was used, and then the hair growth of the subject was observed after 8 months.

Figures 2a to 5b are hair growth before (2a, 3a, 4a, 5a) and after 8 months (2b, 3b, 4b, 5b) using the mixture of oregano oil and natural plant extract according to the present invention This is a picture showing the status.

Referring to FIGS. 2A to 5B, it was visually confirmed that the hair loss part had hair growth at a time point 8 months elapsed after using the mixture of oregano oil and natural plant extract according to the present invention.

In the above, a preferred embodiment of the present invention has been described with reference to the accompanying drawings, but those of ordinary skill in the art to which the present invention pertains, the present invention in other specific forms without changing the technical idea or essential features. It will be appreciated that it can be implemented. Therefore, it should be understood that the exemplary embodiment described above is illustrative in all respects and is not limiting.

Claims (4)

Oregano oil and, in addition to the oregano oil, avocado oil, Amla oil, bhringraj oil, green tea oil, Eoseongcho extract, and natural plant extract consisting of puer tea extract,
The oregano oil is included in a weight ratio of 0.01 to 5.0% by weight based on the total content of the cosmetic composition, and the natural plant extract is included in a weight ratio of 50 to 150 parts by weight based on 100 parts by weight of the oregano oil, and the natural plant extract is 3 to 7 parts by weight of avocado oil, 5 to 10 parts by weight of Amla oil, 1 to 3 parts by weight of bhringraj oil, 4 to 8 parts by weight of green tea oil, Eoseongcho extract 10 To 20 parts by weight and 8 to 12 parts by weight of puer tea extract,
In addition to the oregano oil and natural plant extracts, hyaluronic acid, zinc gluconate, magnesium gluconate, and jade powder are further included, but hyaluronic acid 1 based on 100 parts by weight of the oregano oil and natural plant extract To 3 parts by weight, zinc gluconate (Zinc Gluconate) 2 to 4 parts by weight, magnesium gluconate (Magnesium Gluconate) 3 to 6 parts by weight and jade powder is further included in a weight ratio of 7 to 13 parts by weight,
The oregano oil is prepared by separating and preparing oregano stems and fresh oregano leaves, followed by washing, and drying the washed oregano stems, but in the drying, the washed oregano stems are dried for 4 to 6 days at a temperature of 23 to 27°C, The dried oregano stems are cut into length units of 0.5 to 2.0cm, and the cut oregano stems are mixed with lactic acid bacteria and then matured, but the maturation is the temperature and humidity of the oregano stems mixed with the lactic acid bacteria at 28 to 32°C. Any selected from the group consisting of lactic acid bacteria ( Lactobacillales ), Streptococcus thermophiles ( Streptococcus thermophiles ) and Bifidobacterium longum ( Bifidobacterium longum ) as the lactic acid bacteria are maintained at 50 to 55% for 20 to 30 hours. One lactic acid bacteria is used, and the washed oregano leaves are heated with water vapor, and the heating is performed by heating the washed oregano leaves at a temperature of 125 to 135°C and a pressure of 2 to 3kgf/cm ² for 10 to 30 minutes with water vapor. The raw oregano leaves heated with water vapor are dried for 20 to 40 hours at a temperature of 20 to 25°C, and the dried oregano leaves are heated to roast, but the dried oregano leaves are put into a heating container. After the first roasting for 3 to 5 minutes at a temperature of 70 to 80 °C, the first roasted oregano leaves are secondarily roasted at a temperature of 110 to 120 °C for 20 to 50 seconds, and the roasting The raw oregano leaves are fermented by spraying the raw oregano leaves with the raw oregano leaves, but sprayed at a weight ratio of 10 to 20 parts by weight of the Bacillus bacteria culture solution to 100 parts by weight of the roasted oregano leaves, and a temperature of 50 to 60°C and 60 to 65% After allowing the humidity to be maintained, fermentation is performed for 2 to 4 days, and the Bacillus bacteria culture solution is mixed with 10 to 12% by weight of brown sugar in godubap, After placing eco-friendly rice husks on the table, covering the cloth, and incubating for one week while spraying moisture on the growth twice a day at 25°C, Bacillus is produced, and it is re-inoculated into a medium mixed with rice bran and brown sugar in a 50:50 weight ratio. The Bacillus subtilis , Bacillus subtilis , Bacillus subtilis , Bacillus megaterium , Bacillus megaterium , and Bacillus are prepared by culturing at 25° C. for one week. Any one or more selected from the group consisting of Bacillus licheniformis and Bacillus pumilus is used, and the matured oregano stem and the fermented raw oregano leaves are mixed and fermented to prepare a fermented oregano mixture However, the fermented oregano mixture was mixed in a weight ratio of 300 to 500 parts by weight of the fermented oregano green leaves based on 100 parts by weight of the matured oregano stems, and then 3 to 65 at a temperature of 60 to 65°C and a humidity of 55 to 60%. It is prepared by fermentation for 5 days, and preparing an oregano fermentation broth by mixing a solvent with the fermented oregano mixture and applying ultrasonic waves, the solvent is included in a weight ratio of 300 to 500 parts by weight based on 100 parts by weight of the fermented oregano mixture, Ethanol is used as the solvent, and the ultrasonic wave uses an output of 100 to 200 watts for 50 to 100 minutes at a vibration frequency of 60 to 80 KHz, removes solids from the oregano fermentation broth, and removes the solids The oregano fermentation broth is centrifuged to separate the oregano stalks and the fine particles of the green leaves into a lower layer, and a middle layer and a supernatant located above the lower layer. Oregano oil is produced through the process of extracting the fermented oregano broth, but the oregano oil is 70% in the oregano fermentation broth 2000 to 3000 g (v/v) 1st extraction at 85 to 90°C for 3 to 4 hours with 10 to 15 ℓ of aqueous ethanol solution, and then 4 to 6 liter of 70% (v/v) aqueous ethanol solution at 85 to 90° for 2 to 3 hours at 2 Completing the tea extraction, preparing an extract until the concentration of 1000 to 1500 ml in a vacuum rotary evaporator; And ethanol was added to the extract, diluted to 2 to 3 times by weight, centrifuged at 18,000 to 20,000 rpm for 30 to 40 minutes to remove polymer fibers and solids, and then the collected supernatant was twice with 1.5 to 2.0 times the weight of acetic acid. It is prepared including the step of extracting,
The avocado oil is washed after preparing avocado, and the washed avocado is immersed in grape juice and rancidity inhibitor, but the immersion of the washed avocado is a mixture of the avocado mixed with grape juice and rancidity inhibitor in a weight ratio of 1:1 And the rancidity inhibitor is prepared by mixing vitamin C and salt solution in a weight ratio of 1:1, and the salt solution is a salt solution having a concentration of 1 to 2% by weight, and in 100 parts by weight of the washed avocado The mixture of grape juice and rancidity inhibitor was immersed in a weight ratio of 100 to 120 parts by weight, and then maintained for 10 to 40 minutes, and the avocado immersed in the mixture of grape juice and rancidity inhibitor was taken out and 60 to 32°C at a temperature of 28 to 32°C. By storing for 100 minutes, the components of grape juice and rancidity inhibitors are allowed to penetrate into the avocado, and the avocados in which the components of the grape juice and rancidity inhibitors have penetrated are steamed, but the steaming of the avocados is performed by the ingredients of the grape juice and rancidity inhibitors. It is carried out by steaming the avocado with steam for 50 to 100 minutes, drying the steamed avocado, but drying the steamed avocado is performed for 10 to 20 hours at a temperature of 45 to 50 °C, The dried avocado is washed with one or more acids of acetic acid or citric acid, and a fermentation broth consisting of a culture solution and lactic acid bacteria is sprayed on the washed avocado, but as the lactic acid bacteria, Lactobacillales , Streptococcus thermophiles ) And Bifidobacterium longum ( Bifidobacterium longum ) any one of the lactic acid bacteria selected from the group consisting of is used, the culture solution is peptone 15 to 25 parts by weight, malt extract 5 to 15 parts by weight, sodium citrate 0.5 to 1.5 parts by weight, 0.1 to 0.3 parts by weight of dibasic potassium phosphate, 1 to 3 parts by weight of glucose, and 100 to 200 parts by weight of distilled water The avocado sprayed with the fermentation broth is stored and fermented for 3 to 6 days in a storage container maintaining a temperature of 45 to 50°C and a humidity of 60 to 65%, and the fermented avocado is cold-air dried, but the fermentation Cold air drying of the avocado is carried out by drying the fermented avocado with cold air at a temperature of 3 to 6°C for 5 to 10 hours, and maintaining it at a temperature of 55 to 60°C for 1 to 3 days, and the cold air dried avocado Is prepared through a process of separating the oil after pressing at a humidity of 70 to 80%, a temperature of 120 to 150° C. and 3 to 5 kgf/cm ² ,
The Eoseongcho extract is washed after preparing Eoseongcho, the washed Eoseongcho is pulverized so that the particle diameter is in the range of 500 to 2500㎛, and the pulverized Eoseongcho is mixed with a solvent to prepare a mixture, and then added to an ultrasonic extractor Eoseongcho extract mixture is prepared by applying ultrasonic waves to the mixture at a temperature of 20 to 25°C and a pressure of 0.1 to 0.5kgf/cm ² , but the solvent is in a weight ratio of 1000 to 2000 parts by weight based on 100 parts by weight of the pulverized Eoseongcho And, as the solvent, any one or more solvents selected from water or alcohols having 1 to 4 carbon atoms (C1 to C4) are used, and ultrasonic waves applied to the mixture are applied for 30 to 60 minutes at a vibration frequency of 30 to 50 KHz. It is manufactured by using an output of 100 to 200 watts, removing solids from the Eoseongcho extract mixture, and aging for 1 to 3 hours at a temperature of 10 to 20°C,
The puer tea extract is prepared and washed to remove foreign substances attached to the green puer tea leaves, and then the washed puer tea leaves are dried for 5 to 10 hours at a temperature of 25 to 30°C, and the drying After putting the raw puer tea leaves into a heating container, the first roasting is performed at a temperature of 90 to 100°C for 3 to 5 minutes, and the first roasted puer tea leaves are 2 for 30 to 60 seconds at a temperature of 130 to 140°C. The tea is roasted, and a solvent is mixed with the second roasted puer tea leaves and ultrasonic waves are applied to prepare a puer tea mixture, but the solvent is mixed in a weight ratio of 300 to 600 parts by weight based on 100 parts by weight of the green puer tea leaves. , Water is used as the solvent, and the ultrasonic wave applied to the mixture consisting of the secondary roasted puer tea leaves and the solvent produces an output of 300 to 400 watts for 30 to 60 minutes at a vibration frequency of 60 to 80 KHz. Wool and hair growth comprising oregano oil as an active ingredient, which is prepared by separating and removing solids from the puer tea mixture and then storing and aging for 3 to 7 hours at a temperature of 10 to 15°C. Composition for promoting and preventing hair loss. delete delete delete

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