KR102150479B1 - 세포내 생물발광 공명 에너지 전이를 이용하여 생물활성제에 의한 세포 표적 결합의 인식 - Google Patents

세포내 생물발광 공명 에너지 전이를 이용하여 생물활성제에 의한 세포 표적 결합의 인식 Download PDF

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KR102150479B1
KR102150479B1 KR1020157018230A KR20157018230A KR102150479B1 KR 102150479 B1 KR102150479 B1 KR 102150479B1 KR 1020157018230 A KR1020157018230 A KR 1020157018230A KR 20157018230 A KR20157018230 A KR 20157018230A KR 102150479 B1 KR102150479 B1 KR 102150479B1
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bret
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캐롤린 더블유. 히트코
토마스 커클랜드
토마스 마흐라이트
레이첼 에프. 오하나
매트 로버스
키이스 우드
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프로메가 코포레이션
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    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C40—COMBINATORIAL TECHNOLOGY
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    • C40B40/00—Libraries per se, e.g. arrays, mixtures
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    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
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    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
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    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
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    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
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    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]

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  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
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KR1020157018230A 2012-12-12 2013-12-12 세포내 생물발광 공명 에너지 전이를 이용하여 생물활성제에 의한 세포 표적 결합의 인식 Active KR102150479B1 (ko)

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KR1020207024622A KR102292431B1 (ko) 2012-12-12 2013-12-12 세포내 생물발광 공명 에너지 전이를 이용하여 생물활성제에 의한 세포 표적 결합의 인식

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US201261736429P 2012-12-12 2012-12-12
US61/736,429 2012-12-12
US201361794461P 2013-03-15 2013-03-15
US61/794,461 2013-03-15
US201361880048P 2013-09-19 2013-09-19
US61/880,048 2013-09-19
PCT/US2013/074765 WO2014093677A1 (en) 2012-12-12 2013-12-12 Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer

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KR1020207024622A Active KR102292431B1 (ko) 2012-12-12 2013-12-12 세포내 생물발광 공명 에너지 전이를 이용하여 생물활성제에 의한 세포 표적 결합의 인식

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US (1) US10024862B2 (https=)
EP (2) EP3712154A1 (https=)
JP (4) JP6751294B2 (https=)
KR (2) KR102150479B1 (https=)
CN (2) CN109612981B (https=)
AU (1) AU2013359166B2 (https=)
BR (1) BR112015013710B1 (https=)
CA (2) CA2894435A1 (https=)
DK (1) DK2932267T3 (https=)
ES (1) ES2803503T3 (https=)
PL (1) PL2932267T3 (https=)
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KR102802452B1 (ko) 2023-11-07 2025-05-08 대한민국 LgBiT 단백질을 발현하는 세포주 및 HiBiT 단백질을 발현하는 구제역 바이러스를 이용한 구제역 바이러스 감염 여부 확인방법

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JP7092691B2 (ja) 2022-06-28
BR112015013710B1 (pt) 2022-08-09
US20140194307A1 (en) 2014-07-10
JP2016500263A (ja) 2016-01-12
JP6751294B2 (ja) 2020-09-02
CN104871001B (zh) 2018-09-11
BR112015013710A2 (pt) 2018-11-21
JP7393451B2 (ja) 2023-12-06
SG11201504525VA (en) 2015-07-30
CN104871001A (zh) 2015-08-26
PL2932267T3 (pl) 2020-10-19
JP2019071899A (ja) 2019-05-16
CA3174484A1 (en) 2014-06-19
AU2013359166B2 (en) 2019-09-19
WO2014093677A1 (en) 2014-06-19
AU2013359166A1 (en) 2015-07-09
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