CN109612981B - 使用细胞内生物发光共振能量转移识别生物活性剂的细胞靶标结合 - Google Patents

使用细胞内生物发光共振能量转移识别生物活性剂的细胞靶标结合 Download PDF

Info

Publication number
CN109612981B
CN109612981B CN201810902469.2A CN201810902469A CN109612981B CN 109612981 B CN109612981 B CN 109612981B CN 201810902469 A CN201810902469 A CN 201810902469A CN 109612981 B CN109612981 B CN 109612981B
Authority
CN
China
Prior art keywords
small molecule
bioactive agent
fluorophore
cell
binding
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201810902469.2A
Other languages
English (en)
Chinese (zh)
Other versions
CN109612981A (zh
Inventor
C·W·希特科
T·柯克兰
T·马赫莱特
R·F·奥哈纳
M·罗贝斯
K·伍德
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Promega Corp
Original Assignee
Promega Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Promega Corp filed Critical Promega Corp
Publication of CN109612981A publication Critical patent/CN109612981A/zh
Application granted granted Critical
Publication of CN109612981B publication Critical patent/CN109612981B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/10—Libraries containing peptides or polypeptides, or derivatives thereof
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Organic Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Toxicology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CN201810902469.2A 2012-12-12 2013-12-12 使用细胞内生物发光共振能量转移识别生物活性剂的细胞靶标结合 Active CN109612981B (zh)

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US201261736429P 2012-12-12 2012-12-12
US61/736,429 2012-12-12
US201361794461P 2013-03-15 2013-03-15
US61/794,461 2013-03-15
US201361880048P 2013-09-19 2013-09-19
US61/880,048 2013-09-19
CN201380064252.4A CN104871001B (zh) 2012-12-12 2013-12-12 使用细胞内生物发光共振能量转移识别生物活性剂的细胞靶标结合
PCT/US2013/074765 WO2014093677A1 (en) 2012-12-12 2013-12-12 Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CN201380064252.4A Division CN104871001B (zh) 2012-12-12 2013-12-12 使用细胞内生物发光共振能量转移识别生物活性剂的细胞靶标结合

Publications (2)

Publication Number Publication Date
CN109612981A CN109612981A (zh) 2019-04-12
CN109612981B true CN109612981B (zh) 2022-05-13

Family

ID=50934963

Family Applications (2)

Application Number Title Priority Date Filing Date
CN201810902469.2A Active CN109612981B (zh) 2012-12-12 2013-12-12 使用细胞内生物发光共振能量转移识别生物活性剂的细胞靶标结合
CN201380064252.4A Active CN104871001B (zh) 2012-12-12 2013-12-12 使用细胞内生物发光共振能量转移识别生物活性剂的细胞靶标结合

Family Applications After (1)

Application Number Title Priority Date Filing Date
CN201380064252.4A Active CN104871001B (zh) 2012-12-12 2013-12-12 使用细胞内生物发光共振能量转移识别生物活性剂的细胞靶标结合

Country Status (13)

Country Link
US (1) US10024862B2 (https=)
EP (2) EP3712154A1 (https=)
JP (4) JP6751294B2 (https=)
KR (2) KR102150479B1 (https=)
CN (2) CN109612981B (https=)
AU (1) AU2013359166B2 (https=)
BR (1) BR112015013710B1 (https=)
CA (2) CA2894435A1 (https=)
DK (1) DK2932267T3 (https=)
ES (1) ES2803503T3 (https=)
PL (1) PL2932267T3 (https=)
SG (1) SG11201504525VA (https=)
WO (1) WO2014093677A1 (https=)

Families Citing this family (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8524457B2 (en) 2009-09-22 2013-09-03 William Patterson Method for the selection of specific affinity binders by homogeneous noncompetitive assay
AU2013359166B2 (en) * 2012-12-12 2019-09-19 Promega Corporation Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer
US10067149B2 (en) 2012-12-12 2018-09-04 Promega Corporation Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer
SG11201507306VA (en) 2013-03-15 2015-10-29 Promega Corp Activation of bioluminescence by structural complementation
CN104225611B (zh) * 2013-06-18 2017-06-30 天津键凯科技有限公司 达沙替尼与非线性构型聚乙二醇的结合物
EP4219736A1 (en) * 2014-09-12 2023-08-02 Promega Corporation Internal protein tags
US11365402B2 (en) 2014-09-12 2022-06-21 Promega Corporation Internal protein tags
EP3254113B1 (en) 2015-02-05 2024-10-09 Promega Corporation Luciferase-based thermal shift assays
US11768203B2 (en) * 2016-03-31 2023-09-26 University Of Southern California Highly sensitive and specific luciferase based reporter assay for antigen detection
CN109690316A (zh) * 2016-05-31 2019-04-26 王帅 测定细胞内配体靶蛋白相互作用持久力的方法
EP3535270B3 (en) * 2016-11-01 2024-05-29 Promega Corporation Coelenterazine analogues tethered to energy acceptors
CN110226087A (zh) * 2017-01-27 2019-09-10 国立大学法人大阪大学 生物体物质的检测方法、该方法中使用的化学发光指示剂
US11619628B2 (en) * 2017-02-03 2023-04-04 Osaka University Device and determination system using same
EP3803401A4 (en) 2018-05-30 2022-02-23 Promega Corporation BROAD SPECTRUM KINASE BINDERS
JP7653351B2 (ja) 2018-08-23 2025-03-28 プロメガ コーポレイション セレンテラジン類似体
WO2020048607A1 (en) 2018-09-07 2020-03-12 Université Libre de Bruxelles Srsf2 as rna epigenetic factor
WO2020113036A2 (en) 2018-11-28 2020-06-04 Promega Corporation Reactive peptide labeling
EP3941195A4 (en) 2019-03-20 2023-03-22 Promega Corporation PHOTOAFFINITY PROBES
BR112021020354A2 (pt) * 2019-04-10 2021-12-14 Promega Corp Composições e métodos para detecção de analito com o uso de bioluminescência
CN112147114B (zh) * 2019-06-27 2024-06-25 成都先导药物开发股份有限公司 一种利用荧光标记化合物确定化合物在活细胞中与靶标相互作用的方法
US12061202B2 (en) 2019-12-10 2024-08-13 Promega Corporation Compositions and methods for bioluminescent detection using multifunctional probes
CN116391129A (zh) 2020-08-28 2023-07-04 普罗美加公司 针对ras蛋白的靶标接合测定法
CN115161296B (zh) * 2021-04-01 2024-08-13 深圳华大生命科学研究院 一种刺虾荧光素酶Nluc的突变体及其应用
EP4337646A4 (en) 2021-05-13 2025-05-21 Promega Corporation BIOLUMINESCENT DETECTION OF DNA SYNTHESIS
CN114394970B (zh) * 2022-01-05 2023-06-06 山东大学 一种酯类Furimazine衍生物及制备方法与应用
CN114813706B (zh) * 2022-06-29 2022-12-13 国科大杭州高等研究院 一种血细胞高光谱光镊捕获能量共振转移分析仪
KR20250085812A (ko) 2022-10-14 2025-06-12 프로메가 코포레이션 코엘렌테라진 유사체
CN116354927B (zh) * 2023-02-21 2025-02-07 杭州医学院 以烷烃或脂肪酸为连接链的hdac降解剂及其制备方法和应用
AU2024265176A1 (en) * 2023-05-04 2025-12-11 Promega Corporation Complementation-based tags and reporters for dual-modality labeling
KR20250004513A (ko) 2023-06-29 2025-01-08 부산대학교 산학협력단 Bret-기반 rab11a 활성 바이오센서 및 이를 포함하는 세포주
CN117330755B (zh) * 2023-08-25 2024-03-26 广东省农业科学院农业生物基因研究中心 一种基于酶催化生物荧光定量检测dna-蛋白质相互作用的方法
KR102802452B1 (ko) 2023-11-07 2025-05-08 대한민국 LgBiT 단백질을 발현하는 세포주 및 HiBiT 단백질을 발현하는 구제역 바이러스를 이용한 구제역 바이러스 감염 여부 확인방법
WO2025137564A1 (en) 2023-12-20 2025-06-26 Promega Corporation Coelenterazine analogues
GB202410534D0 (en) 2024-07-18 2024-09-04 Nrg Therapeutics Ltd Novel method and uses
CN119709661B (zh) * 2024-12-30 2025-10-10 佰翱得(无锡)新药开发有限公司 一种荧光素酶OLuc突变体及其应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101962390A (zh) * 2003-04-02 2011-02-02 圣瑞细胞荧光技术有限公司 荧光标记的配体

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5928888A (en) * 1996-09-26 1999-07-27 Aurora Biosciences Corporation Methods and compositions for sensitive and rapid, functional identification of genomic polynucleotides and secondary screening capabilities
CA2335305C (en) 1998-06-16 2006-05-23 Biosignal Packard Inc. A bioluminescence resonance energy transfer (bret) system and its use
WO2001046691A1 (en) 1999-12-22 2001-06-28 Biosignal Packard Inc. A bioluminescence resonance energy transfer (bret) system with broad spectral resolution between donor and acceptor emission wavelengths and its use
WO2002029410A2 (en) 2000-10-06 2002-04-11 Quantum Dot Corporation Cells having a spectral signature, and methods of preparation and use thereof
DE10058091A1 (de) * 2000-11-23 2002-06-06 Bayer Ag Isolierte Luziferasen Lu164, LuAL und Lu22, sowie deren Verwendung
WO2004034054A2 (en) * 2002-10-11 2004-04-22 7Tm Pharma A/S Method for enhancing and prolonging the bioluminescence resonance energy transfer (bret) signal in a bret assay and a substrate solution for use in a bret assay
CN100473988C (zh) * 2004-07-08 2009-04-01 厦门大学 具有荧光共振能量转移特点的蛋白组合及其用途
WO2006086883A1 (en) * 2005-02-16 2006-08-24 Universite De Montreal Biosensors for monitoring receptor-mediated g-protein activation
GB0625678D0 (en) * 2006-12-21 2007-01-31 Lux Biotechnology Ltd Composition and method for detection of demineralisation
WO2008109463A2 (en) * 2007-03-02 2008-09-12 University Of Massachusetts Red-shifted luciferase
EP2006686A1 (en) * 2007-06-22 2008-12-24 Institut Pasteur Method for screening for selective modulator of the NF-kB pathway activation
EP2215227A2 (en) * 2007-11-05 2010-08-11 Promega Corporation Hybrid fusion reporter and uses thereof
WO2010068747A1 (en) * 2008-12-12 2010-06-17 University Of Florida Research Foundation, Inc. Cell-based detection of apf through its interaction with ckap4 for diagnosis of interstitial cystitis
LT3409764T (lt) 2009-05-01 2020-06-10 Promega Corporation Sintetinės oplophorus liuciferazės su sustiprinta šviesos išeiga
US8263350B2 (en) * 2009-06-29 2012-09-11 The University Of Chicago Molecular affinity clamp technology and uses thereof
BR112013010487B1 (pt) 2010-11-02 2021-02-02 Promega Corporation compostos de coelenterazina, kit compreendendo ditos compostos e método para detectar luminescência em uma amostra in vitro
EP2782916B1 (en) * 2011-11-21 2018-02-28 Promega Corporation Carboxy x rhodamine analogs
CN102798717B (zh) * 2012-06-15 2014-11-26 杭州师范大学 一种o6-甲基鸟嘌呤-dna甲基转移酶活性检测方法
AU2013359166B2 (en) 2012-12-12 2019-09-19 Promega Corporation Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer
US10067149B2 (en) 2012-12-12 2018-09-04 Promega Corporation Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101962390A (zh) * 2003-04-02 2011-02-02 圣瑞细胞荧光技术有限公司 荧光标记的配体

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Engineered Luciferase Reporter from a Deep Sea Shrimp Utilizing a Novel Imidazopyrazinone Substrate;Mary P.Hall et al;《ACS Chem. Biol.》;20120815;第7卷;第1848-1857页 *

Also Published As

Publication number Publication date
JP7092691B2 (ja) 2022-06-28
BR112015013710B1 (pt) 2022-08-09
US20140194307A1 (en) 2014-07-10
JP2016500263A (ja) 2016-01-12
JP6751294B2 (ja) 2020-09-02
CN104871001B (zh) 2018-09-11
BR112015013710A2 (pt) 2018-11-21
JP7393451B2 (ja) 2023-12-06
SG11201504525VA (en) 2015-07-30
CN104871001A (zh) 2015-08-26
KR102150479B1 (ko) 2020-09-01
PL2932267T3 (pl) 2020-10-19
JP2019071899A (ja) 2019-05-16
CA3174484A1 (en) 2014-06-19
AU2013359166B2 (en) 2019-09-19
WO2014093677A1 (en) 2014-06-19
AU2013359166A1 (en) 2015-07-09
CN109612981A (zh) 2019-04-12
KR102292431B1 (ko) 2021-08-20
EP2932267B1 (en) 2020-05-06
JP2022078077A (ja) 2022-05-24
ES2803503T3 (es) 2021-01-27
JP2023179413A (ja) 2023-12-19
EP2932267A4 (en) 2016-06-22
DK2932267T3 (da) 2020-07-20
JP7676487B2 (ja) 2025-05-14
EP2932267A1 (en) 2015-10-21
CA2894435A1 (en) 2014-06-19
US10024862B2 (en) 2018-07-17
KR20200104928A (ko) 2020-09-04
KR20150094696A (ko) 2015-08-19
EP3712154A1 (en) 2020-09-23

Similar Documents

Publication Publication Date Title
CN109612981B (zh) 使用细胞内生物发光共振能量转移识别生物活性剂的细胞靶标结合
US20230417778A1 (en) Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer
Blay et al. High-throughput screening: today’s biochemical and cell-based approaches
US9952208B2 (en) Means and methods for bioluminescence resonance energy transfer (BRET) analysis in a biological sample
Watanabe et al. Multicolor protein labeling in living cells using mutant β-lactamase-tag technology
JP2020062032A (ja) 二重蛍光体を用いた酵素アッセイに使用するための細胞
Arts et al. Engineering BRET-sensor proteins
Saca et al. GPCR biosensors to study conformational dynamics and signaling in drug discovery
Chen et al. Single-chain lanthanide luminescence biosensors for cell-based imaging and screening of protein-protein interactions
US9915650B2 (en) Method of detection of cAMP and cGMP via a fluorophore and dequencher complex
Heuninck et al. Time-resolved FRET-based assays to characterize G protein-coupled receptor hetero-oligomer pharmacology
Nesakumar et al. Development of Ligand Assay Systems Using Semisynthetic Biosensors Based on Protein
WO2024151471A1 (en) High-throughput assay based on ligand-biased structural dynamics response
Payne Development of CoraFluors: A Versatile, Target-agnostic Assay Technology Platform Optimized for Chemical Biology Research
Chen T Luminescence Imaging and Biosensing in Cells and Tissues

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant