KR102142065B1 - How to grow the inside of a bottle of matsutake mushroom using grain medium - Google Patents

Abstract

The present invention provides a method for cultivating blossom mushrooms, which can be grown aseptically in a grain medium and then used together with the blossom mushrooms as a raw material for food.
The method for cultivating the blossom mushrooms of the present invention is to increase the beta-clucan content in the blossom mushrooms by using grain medium, and to use the blossom mushrooms with the increased beta-glucan content in food.
The method for cultivating the blossom mushrooms using the grain medium of the present invention is mixed with 50 to 100 parts by weight of the grain medium and 10 to 20 parts by weight of the liquid seed fungus in the PP bottle of 850 cc capacity in the grain medium, and the relative humidity of the medium is 60 to 90%. Cultivate for 60-75 days.

Description

How to cultivate the inside of a bottle of matsutake mushroom using grain medium{.}

The present invention relates to an inner cultivation method for growing cultivated mushrooms by cultivating a grain medium on a sterile bottle medium. The blossom mushrooms are cultivated to be used as a raw material for food, etc. along with the grain medium.

In general, mushrooms (mushroom) has a unique scent and taste, so it is widely used in foods around the world. Mushrooms contain various enzymes to enhance immunity, improve blood circulation, activate immune cells, and have anti-cancer effects. It is known and reported to the society.

Since mushrooms are difficult to harvest in nature and have low yields, it is common to grow mushroom fruiting bodies using sawdust or trees in greenhouses such as plastic greenhouses.

Blossom Mushroom is a mushroom of the genus genus Mushroom of the Basidiomycetes. It is native to Korea, Japan, Europe, North America, and Australia, and grows mainly on the stumps of trees and coniferous trees.

These blossoms contain more than 40 g of beta glucan in 100 g. The beta-glucan is known to be useful for various diseases such as cancer, hypertension, diabetes, and kidney disease as a component that enhances the immune system of the human body.

In Korean Patent Publication No. 10-1004645, the seed is inoculated with liquid spawn and cultured for 2 to 3 months, and satisfactory results cannot be obtained from the culture speed or collection amount. In Korean Patent Publication No. 10-2010-0066321, rice, brown rice, and barley There is a problem in that the method of inoculating and culturing a grain medium consisting of wheat, corn, crude, etc. is insufficient, or the growth rate of the mycelium is insufficient. In addition, since the conventional culture method of cultivating mushroom mycelium using grain medium is washed with grain → immersion → bottling → sterilization → cooling → inoculation process and culture temperature is 0~30℃, and culture is 30~40 days, productivity and economic efficiency It does not reach profitability through mushroom cultivation.

After cultivating mushrooms and harvesting mushrooms using the conventional mushroom cultivation method as described above, the mushroom medium has been discarded as a waste form, except when used as a raw material for some feeds.

EP 1724824 A2

The present invention has been devised to solve the problems of the prior art as described above, and the cultivation of blossom mushrooms in aseptic condition in the grain medium, and then the blossom mushrooms and medium can be used together as raw materials for food, such as blossom mushrooms, inside the bottle growing method Gives

For another purpose, the method for cultivating the blossom mushrooms of the present invention provides a purpose for increasing the beta-clucan content in the blossom mushrooms by using grain medium, and using the blossom mushrooms with the increased beta-glucan content in food.

The blossoming mushroom bottle cultivation method using the grain medium to achieve the object of the present invention as described above, put 50 to 100 parts by weight of the grain medium in a PP (polypropylene) bottle with a capacity of 850 cc, and 10 to 20% by weight of the liquid mushroom fungus on the grain medium. After inoculation, cultivation is performed for 60-75 days at 60-90% relative humidity of the medium.

The grain medium is crushed into grain raw materials consisting of rice, brown rice, black rice, barley, wheat, corn, crude, oats and soybeans or particles of 0.001 to 0.5 mm, and then used grain medium. After placing the grain medium in a 850cc PP bottle and adding water, sterilize it for 15-30 minutes at a temperature of 110 to 125°C (preferably 121°C) at a temperature of 110 to 125°C (preferably 121°C) for 15 to 30 minutes. After cooling to a temperature of ~20℃, inoculate the liquid seed in aseptic condition. Inoculate the liquid medium of the mushrooms on the grain medium, and when adding water, add 50~150ml of water to moisturize.

The conditions for culturing the hyphae after inoculating the liquid seed are culturing the initial mycelium in the dark condition for 8 to 12 days at a temperature of 23 to 25°C and a humidity of 68 to 75% for the PP bottle, and the temperature of 23 to 25°C and humidity 78 After culturing the late mycelium under light conditions for 18-22 days at ~82%, blossom mushrooms are grown under light conditions for 34-41 days at a temperature of 18-20°C and 80-90% humidity.

The blossoming mushroom bottle cultivation method using the grain medium of the present invention provides an intra-bottle growing method for providing the grain medium as it is as a raw material such as food.

In addition, the blossom mushroom of the present invention is for cultivating excellent blossom mushrooms by increasing the content of beta-glucan using a grain medium.

In addition, according to the present invention, as the sterile mushrooms are cultivated under the optimum conditions of cultivation of the mushrooms under environmental conditions such as temperature, humidity and light conditions, the mushrooms can be cultivated as soon as possible, and the period of fruiting and mushroom growth can be accelerated as soon as possible It can be done quickly, thereby reducing the cost of cultivation, and has the effect of making the market competitive.

Hereinafter, the present invention will be described in detail.

The blossom mushrooms of the present invention are grown in aseptic conditions using a grain medium, and then the grain medium itself can be used as a raw material for food, etc., along with the blossom mushrooms.

The method for cultivation of blossom mushrooms using the grain medium of the present invention is to put 50 to 100 parts by weight of the grain medium in a PP (polypropylene) bottle, and then inoculate 10 to 20 parts by weight of the liquid mushroom fungus into the PP bottle containing the grain medium. Cultivation at 60-90% relative humidity for 60-75 days.

The fruiting body of the mushrooms is quickly formed at a temperature of 18~20℃, and the growth of hyphae grows well around 24℃. The hyphae growth stage requires 60% to 70% humidity, and the fruiting stage growth stage requires relatively high humidity, so the relative humidity in the air must be maintained at 80% or higher. Normally, the humidity in the air should be at least 90%, but since the present invention is cultivated inside a PP bottle, an air humidity of 80% or more is sufficient.

When heated to maintain the relative humidity of the cultivation room where the cultivation of zinnia mushrooms is heated, the cultivation room and the medium are sterilized using ozone water to prevent the medium of the cultivation room and the blossoming mushroom from decaying or contaminating.

When humidifying to maintain relative humidity, water is humidified with ozone water having an ozone concentration of 0.03 to 0.2 ppm to keep the room in the cultivation room sterile at all times. In addition, the water supplied to the medium is also prevented from decaying by using ozone water having an ozone concentration of 0.05 to 0.5 ppm.

In addition, it is possible to disinfect the air and the medium in the cultivation room using ultraviolet rays of 200 to 300 nm, and it is more effective when used with the ozone water.

The mycelial growth of zinnia mushrooms is irrespective of weak light, dark light, or matte at the time of poem, but requires light induction at the fruiting stage. Growth of fruiting bodies was actively induced under light intensity of 500-800 lux.

Blossom mushrooms grow under relatively acidic conditions, and when the pH value of the incubator is within the range of 3.5-7, the mycelium grows normally and the optimal pH is 4-5, and when the pH value exceeds 7.5, the mycelium growth is disturbed and pH3. Mycelium is difficult to grow below 0.

After inoculation, incubate at a culture temperature of 22℃-25℃ in the culture room. In the culture room, avoid possible light rays and maintain the relative humidity in the air at 80% or less. Avoid overheating and high temperature to revitalize mycelium and prevent contamination. During the cultivation period, through regular inspection, the medium with weak contaminants and power is removed. Mycelium prevails around 30 days after culture.

In the first 8 to 12 days of mycelial culture, the mycelium grows under no need of light source or weak light. After 35 days, the slope mycelium begins to burn on the wall, and at this time, irradiation of diffused light induces differentiation and promotes mycelial growth from reproductive growth to reproductive growth. Hyphae growth cannot be completed under dark conditions, and hyphae growth is suppressed under strong light. Normally, 300 to 500 lux of diffused light is beneficial for mycelial growth. After about a month, the energy appears, and the mushrooms must be transferred to the cultivation room.

Blossom mushrooms are prepared for mushroom development only after about 30 days have passed before the mushrooms are generated only by the cooperative action of three factors: temperature, humidity and light source in the cultivation room.

When the mycelial culture is 26~35, the temperature is 23~25℃, the relative humidity in the air is 80% or less, and the intensity of the light source is advantageous for the formation of hyphae in the light of 500~800lux.

It takes about 20 days from the formation of eruption to the formation of differentiation, and the cultivation temperature should be controlled to 18~20℃ and the relative humidity is maintained at 80~90%. During this period, it is necessary to irradiate with light, and the light intensity needs to be 800~1000 lux. Under these environmental conditions, after 30 days of cultivation, fruiting bodies differentiate and small leaves appear.

The maturity status of fruiting mushrooms has no special characteristics, and it is necessary to determine the harvesting time by looking at the appearance, such as the value of eggplant products, but the following two criteria can be used. The harvesting time is harvested when the cluster shape is unfolded, the blue state around the leaves, or white villi on the back, and when the color of the fruiting body leaves turn white to pale yellow. Spraying should be stopped 12 hours prior to harvest.

If drying is done to make dried mushrooms, the fruits are dried under hot air or sunlight after harvesting, and the temperature of the fruits must be strictly controlled. The initial drying time starts at 30°C and is dried for about 24 hours to 50°C. Above that temperature, the fruiting body may burn or lose color and odor.

The grain medium uses grain itself (raw material) composed of rice, brown rice, black rice, barley, wheat, corn, crude, oats, and soybeans or grain medium crushed into particles having a size of 0.001 to 0.5 mm.

The grain medium is 10-15 parts by weight of rice, 20-30 parts by weight of brown rice, 5-10 parts by weight of black rice, 10-20 parts by weight of barley, 1-5 parts by weight of wheat, 1-5 parts by weight of corn, 1-5 parts of crude Grain medium mixed with 1 part by weight, 1-5 parts by weight of oats, and 1-5 parts by weight of beans is used. The mixing ratio is the preferred mixing ratio that makes the growth of zinnia mushrooms most vigorous and can generate the highest content of beta-glucan in the body of zinnia mushrooms.

After placing the grain medium in a 850cc PP bottle and adding water, use a high temperature autoclave for 110 to 125℃, preferably 121℃ and 1.0 to 1.5 atm (preferably 1.2 atm) for 15 to 30 minutes. After sterilization, it is cooled to a temperature of 10 to 20°C, and then inoculated with liquid spawn in aseptic conditions.

The concentration required to sterilize bacteria using ozone in a water purification plant is about 0.2PPM, and 99% of all bacteria are killed at this concentration (actually treated with 0.4-0.5PPM to enhance the effect), and E. coli is 0.25-0.03PPM Most are killed.

At an ozone concentration of 0.3-0.5 PPM, fungal, yeast, and archaea bacteria die within seconds to minutes, and various viruses such as E. coli, Staphylococcus aureus, Pseudomonas aeruginosa, and various inflation enzymes die within 5 seconds, and sterilization power is the fungus of the dormant period. They range from germs and bacterial spores. In other words, the sterilizing power of ozone shows a wide range of sterilizing power not only to various fungi, but also to bacteria and viruses such as anthrax, and it is possible to grow the medium aseptically so that the mushrooms can be used for food and the like.

The conditions for culturing the fruiting body after inoculating the liquid seed, cultivating the initial mycelia in the dark condition for 8 to 12 days at a temperature of 23 to 25°C and a humidity of 68 to 75% in a PP bottle containing the grain medium inoculated with the liquid seed. And incubate the late mycelium under light conditions for 18 to 22 days at 23 to 25°C and humidity at 78 to 82%, then blossom under bright conditions for 34 to 41 days at temperature 18 to 20°C and 80 to 90% relative humidity. Grow mushrooms.

Claims (4)

In the method for cultivating the inside of a bottle of matsutake mushroom using grain medium,
The inner cultivation method of the blossom mushroom bottle is 50 to 100 parts by weight of the grain medium in a PP (polypropylene) bottle with a capacity of 850 cc, and then mixed with 10 to 20 parts by weight of the liquid seed fungus in the PP bottle containing the grain medium. Cultivated for 60-75 days at 60-90% relative humidity,
The grain medium is 10-15 parts by weight of rice, 20-30 parts by weight of brown rice, 5-10 parts by weight of black rice, 10-20 parts by weight of barley, 1-5 parts by weight of wheat, 1-5 parts by weight of corn, 1-5 parts of crude The raw material itself, or 1 to 5 parts by weight of oats, 1 to 5 parts by weight of beans, or the mixed raw material is pulverized into particles having a size of 0.001 to 0.5 mm and mixed and used.
The conditions for culturing fruiting bodies after mixing the liquid strains are culturing the initial mycelium under dark conditions for 8 to 12 days in a PP bottle at a temperature of 23 to 25°C and a humidity of 68 to 75%,
After culturing late mycelium under light conditions for 18 to 22 days at a temperature of 23 to 25°C and humidity of 78 to 82%, cultivation of mushrooms under bright conditions for 34 to 41 days at a temperature of 18 to 20°C and humidity of 80 to 90% A method of cultivating the inside of a bottle of matsutake mushroom using a grain medium.
delete According to claim 1,
After placing the grain medium in a 850cc PP bottle and adding water, sterilize it for 15 to 30 minutes at a temperature of 110 to 125°C under a condition of 1.0 to 1.5 atm using a high-temperature autoclave and then cool it to a temperature of 10 to 20°C After inoculation, the method for cultivating the inside of a bottle of blossom mushroom using grain medium, characterized by inoculating the liquid seed in a sterile state.





delete