KR102115360B1 - 성체 줄기세포 배양액 및 그 제조방법 - Google Patents

성체 줄기세포 배양액 및 그 제조방법 Download PDF

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KR102115360B1
KR102115360B1 KR1020180061483A KR20180061483A KR102115360B1 KR 102115360 B1 KR102115360 B1 KR 102115360B1 KR 1020180061483 A KR1020180061483 A KR 1020180061483A KR 20180061483 A KR20180061483 A KR 20180061483A KR 102115360 B1 KR102115360 B1 KR 102115360B1
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South Korea
Prior art keywords
culture
medium
stem cells
cells
cell culture
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KR1020180061483A
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English (en)
Korean (ko)
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KR20190009695A (ko
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박재우
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주식회사 바이블리오테카
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Priority to KR1020180061483A priority Critical patent/KR102115360B1/ko
Publication of KR20190009695A publication Critical patent/KR20190009695A/ko
Priority to MX2020012696A priority patent/MX2020012696A/es
Priority to PCT/KR2019/004906 priority patent/WO2019231111A1/ko
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Publication of KR102115360B1 publication Critical patent/KR102115360B1/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0667—Adipose-derived stem cells [ADSC]; Adipose stromal stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
    • A61K8/981—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of mammals or bird
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/02—Atmosphere, e.g. low oxygen conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/12—Hepatocyte growth factor [HGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/15—Transforming growth factor beta (TGF-β)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/165—Vascular endothelial growth factor [VEGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2513/00—3D culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2527/00—Culture process characterised by the use of mechanical forces, e.g. strain, vibration

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Birds (AREA)
  • Epidemiology (AREA)
  • Dermatology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Rheumatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
KR1020180061483A 2018-05-30 2018-05-30 성체 줄기세포 배양액 및 그 제조방법 KR102115360B1 (ko)

Priority Applications (3)

Application Number Priority Date Filing Date Title
KR1020180061483A KR102115360B1 (ko) 2018-05-30 2018-05-30 성체 줄기세포 배양액 및 그 제조방법
MX2020012696A MX2020012696A (es) 2018-05-30 2019-04-23 Un fluido de cultivo de celulas madre adultas y un metodo de preparacion del mismo.
PCT/KR2019/004906 WO2019231111A1 (ko) 2018-05-30 2019-04-23 성체 줄기세포 배양액 및 그 제조방법

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1020180061483A KR102115360B1 (ko) 2018-05-30 2018-05-30 성체 줄기세포 배양액 및 그 제조방법

Publications (2)

Publication Number Publication Date
KR20190009695A KR20190009695A (ko) 2019-01-29
KR102115360B1 true KR102115360B1 (ko) 2020-05-26

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KR1020180061483A KR102115360B1 (ko) 2018-05-30 2018-05-30 성체 줄기세포 배양액 및 그 제조방법

Country Status (3)

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KR (1) KR102115360B1 (es)
MX (1) MX2020012696A (es)
WO (1) WO2019231111A1 (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102115360B1 (ko) * 2018-05-30 2020-05-26 주식회사 바이블리오테카 성체 줄기세포 배양액 및 그 제조방법
KR20230127939A (ko) * 2022-02-25 2023-09-01 주식회사 강스템바이오텍 마이크로캐리어를 이용한 줄기세포 배양 방법

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050054101A1 (en) * 2003-07-17 2005-03-10 Global Cell Solutions Llc Automated cell culture system and process

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100871652B1 (ko) * 2007-07-30 2008-12-02 한양대학교 산학협력단 세포 배양용 3차원 마이크로 캐리어, 이의 제조방법 및이를 이용한 세포의 3차원 부유 배양법
WO2010059775A1 (en) * 2008-11-20 2010-05-27 Centocor Ortho Biotech Inc. Pluripotent stem cell culture on micro-carriers
KR101639988B1 (ko) * 2011-05-11 2016-07-15 (주)안트로젠 고농도의 세포성장인자를 포함하는 중간엽 줄기세포 배양액의 제조방법 및 이로부터 얻어진 조성물
KR101539822B1 (ko) * 2012-05-14 2015-07-29 서울대학교산학협력단 줄기세포의 3차원 배양을 통한 고농축 배양액의 제조방법 및 고농축 배양액의 용도
KR20180128529A (ko) * 2013-11-01 2018-12-03 얀센 바이오테크 인코포레이티드 췌장 내분비 세포로의 분화를 위한 인간 만능 줄기세포의 현탁 및 클러스터링
KR102115360B1 (ko) * 2018-05-30 2020-05-26 주식회사 바이블리오테카 성체 줄기세포 배양액 및 그 제조방법

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050054101A1 (en) * 2003-07-17 2005-03-10 Global Cell Solutions Llc Automated cell culture system and process

Also Published As

Publication number Publication date
MX2020012696A (es) 2021-02-15
KR20190009695A (ko) 2019-01-29
WO2019231111A1 (ko) 2019-12-05

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