MX2020012696A - Un fluido de cultivo de celulas madre adultas y un metodo de preparacion del mismo. - Google Patents

Un fluido de cultivo de celulas madre adultas y un metodo de preparacion del mismo.

Info

Publication number
MX2020012696A
MX2020012696A MX2020012696A MX2020012696A MX2020012696A MX 2020012696 A MX2020012696 A MX 2020012696A MX 2020012696 A MX2020012696 A MX 2020012696A MX 2020012696 A MX2020012696 A MX 2020012696A MX 2020012696 A MX2020012696 A MX 2020012696A
Authority
MX
Mexico
Prior art keywords
cell culture
culture fluid
stem cell
preparation
mesenchymal stem
Prior art date
Application number
MX2020012696A
Other languages
English (en)
Inventor
Jae Woo Park
Original Assignee
Viblioteca Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Viblioteca Co Ltd filed Critical Viblioteca Co Ltd
Publication of MX2020012696A publication Critical patent/MX2020012696A/es

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0652Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662Stem cells
    • C12N5/0667Adipose-derived stem cells [ADSC]; Adipose stromal stem cells
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/98Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/98Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
    • A61K8/981Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of mammals or bird
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00Preparations for care of the skin
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/02Atmosphere, e.g. low oxygen conditions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/12Hepatocyte growth factor [HGF]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/15Transforming growth factor beta (TGF-β)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/165Vascular endothelial growth factor [VEGF]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2513/003D culture
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2527/00Culture process characterised by the use of mechanical forces, e.g. strain, vibration

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Birds (AREA)
  • Epidemiology (AREA)
  • Dermatology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Rheumatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Tarea: preparar un fluido de cultivo de células madre mesenquimales de forma económica y sencilla. Solución: En la presente invención, se puede obtener un fluido de cultivo de células madre mesenquimatosas que contiene un alto contenido de citocinas y factores de crecimiento celular durante un largo tiempo cultivando células madre mesenquimatosas en un medio sin suero usando un microvehículo como soporte de unión.
MX2020012696A 2018-05-30 2019-04-23 Un fluido de cultivo de celulas madre adultas y un metodo de preparacion del mismo. MX2020012696A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020180061483A KR102115360B1 (ko) 2018-05-30 2018-05-30 성체 줄기세포 배양액 및 그 제조방법
PCT/KR2019/004906 WO2019231111A1 (ko) 2018-05-30 2019-04-23 성체 줄기세포 배양액 및 그 제조방법

Publications (1)

Publication Number Publication Date
MX2020012696A true MX2020012696A (es) 2021-02-15

Family

ID=65323277

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2020012696A MX2020012696A (es) 2018-05-30 2019-04-23 Un fluido de cultivo de celulas madre adultas y un metodo de preparacion del mismo.

Country Status (3)

Country Link
KR (1) KR102115360B1 (es)
MX (1) MX2020012696A (es)
WO (1) WO2019231111A1 (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102115360B1 (ko) * 2018-05-30 2020-05-26 주식회사 바이블리오테카 성체 줄기세포 배양액 및 그 제조방법
KR20230127939A (ko) * 2022-02-25 2023-09-01 주식회사 강스템바이오텍 마이크로캐리어를 이용한 줄기세포 배양 방법

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005010162A2 (en) * 2003-07-17 2005-02-03 Global Cell Solutions, Llc. Automated cell culture system and process
KR100871652B1 (ko) * 2007-07-30 2008-12-02 한양대학교 산학협력단 세포 배양용 3차원 마이크로 캐리어, 이의 제조방법 및이를 이용한 세포의 3차원 부유 배양법
EP2366021B1 (en) * 2008-11-20 2017-08-02 Janssen Biotech, Inc. Pluripotent stem cell culture on micro-carriers
KR101639988B1 (ko) * 2011-05-11 2016-07-15 (주)안트로젠 고농도의 세포성장인자를 포함하는 중간엽 줄기세포 배양액의 제조방법 및 이로부터 얻어진 조성물
KR101539822B1 (ko) * 2012-05-14 2015-07-29 서울대학교산학협력단 줄기세포의 3차원 배양을 통한 고농축 배양액의 제조방법 및 고농축 배양액의 용도
JP2016534731A (ja) * 2013-11-01 2016-11-10 ヤンセン バイオテツク,インコーポレーテツド 膵内分泌細胞への分化のためのヒト多能性幹細胞の懸濁及び集団化
KR102115360B1 (ko) * 2018-05-30 2020-05-26 주식회사 바이블리오테카 성체 줄기세포 배양액 및 그 제조방법

Also Published As

Publication number Publication date
KR102115360B1 (ko) 2020-05-26
KR20190009695A (ko) 2019-01-29
WO2019231111A1 (ko) 2019-12-05

Similar Documents

Publication Publication Date Title
CY1119924T1 (el) Μεθοδος για παρασκευη ενος βασικου μεσου καλλιεργειας για μεσεγχυματικα βλαστοκυτταρα, βασικο μεσο καλλιεργειας για μεσεγχυματικα βλαστοκυτταρα, και κυτταρικος θεραπευτικος παραγοντας καλλιεργημενος και διαφοροποιημενος με χρηση αυτου
EP4148121A3 (en) Cell-controlled perfusion in continuous culture
PH12015501465A1 (en) Culturing of human embryonic stem cells at the air-liquid interface for differentiation into pancreatic endocrine cells
MX2022010521A (es) Metodo para producir linfocitos citoliticos naturales a partir de celulas madre pluripotentes.
ATE525460T1 (de) Verfahren zur kultivierung und vermehrung hämatopoetischer stammzellen und vorläuferzellen unter verwendung menschlicher endometriumzellen
MX2013014937A (es) Cultivo de celulas de mamifero.
WO2011159359A3 (en) Serum-free chemically defined cell culture medium
MX2020012696A (es) Un fluido de cultivo de celulas madre adultas y un metodo de preparacion del mismo.
RU2012121842A (ru) Способ получения мезенхимальных стволовых клеток из плюрипотентных стволовых клеток человека и мезенхимальные стволовые клетки, полученные этим способом
EA202091407A1 (ru) Состав бессывороточной среды для культивирования клеток и способы его применения
EP3940062A4 (en) COSMETIC COMPOSITION COMPRISING A SOLUTION FOR CULTURE OF MESENCHYMATOUS STEM CELLS CULTURED IN A MEDIUM CONTAINING HPL
MY161151A (en) Method and bioreactor for the cultivation of microorganisms
MX2016013223A (es) Formulacion en polvo de medio de compuesto y metodo de preparacion de medio liquido para cultivo celular.
JP2012143229A5 (es)
EA201501002A1 (ru) Способ культивирования гепатобластподобных клеток и продукт их культивирования
WO2021009778A3 (en) Methods for culturing mesenchymal stem cells, products thereof, and applications thereof
WO2013141731A3 (en) Process for ex-vivo expansion of hematopoietic stem cells in a bioreactor
AR077217A1 (es) Medios para cultivo de celulas de mamiferos que comprenden sobrenadante de etapas del fraccionamiento de cohn y uso de los mismos
EP3848455A4 (en) Medium for direct differentiation of pluripotent stem cell-derived mesenchymal stem cell, method for preparing mesenchymal stem cell by using same, and mesenchymal stem cell prepared thereby
SG11201806843UA (en) Method for producing activated hepatocyte growth factor (hgf)
PH12018502223A1 (en) Method for producing activated stem cells
EP4235175A3 (en) Methods for culturing human keratinocytes
EA201992828A1 (ru) Способы культивирования клеток
MY192147A (en) Method for obtaining high-yield, stable expression cell clones and antibody molecules obtained thereby
EP3694700A4 (en) HOLLOW MICRO SUPPORT FOR SHEAR-FREE CULTURE OF ADHERENT CELLS IN BIOREACTORS