KR102092509B1 - Food composition for preventing and improving of dementia - Google Patents

Abstract

The present invention relates to hot water extracts of 8 kinds of medicines, such as saengjihwang root, saengjihwang root, Baekbokryeong root, white wheat (honey), goji berry, cornus berry, Omija fruit, and white sorghum root. There is an excellent effect that can be used as a health supplement functional food composition containing a concentrate of seven kinds of herbal plants and hot water extract of white wheat as an effective ingredient.

Description

Food composition for preventing and improving dementia

The present invention prevents and improves immunity enhancement or dementia that contains 7 kinds of herbal plants, such as raw rhizome root, red ginseng root, Baekbokryeong root, goji berry fruit, cornus fruit, Omija fruit, and white sorghum root and hot water extract of white wheat (honey) as an active ingredient. The present invention relates to a functional food composition, and more particularly, to a food composition for preventing and improving dementia, such as Alzheimer's, using the seven kinds of herbal plants and hot water extract of white wheat.

In recent industrialization and changes in the environment, modern people are exposed to various diseases due to reduced immunity caused by various causes, and they are consuming enormous costs to treat these diseases. In particular, for allergic diseases due to recently polluted air and changes in new life, allergens excessively promote the human immune system by various allergens, causing TNF-α (tumor necrosis factor-α), IL in immune cells such as macrophages. Inflammatory substances such as -1 (interleukin-1), IL-6 (interleukin-6), prostaglandin, luecotriene, matrix metalloproteinase-9 (MMP-9) and nitric oxide (NO) It is known that by inducing excessively, it progresses to an inflammatory reaction and causes various diseases and immune hypersensitivity symptoms.

In addition, dementia develops as brain function is impaired due to damage and loss of nerve cells due to the collapse of the immune system, and the onset time of dementia is gradually increasing.

Dementia is a kind of syndrome in which a person who has been living normally has brain function impaired due to various causes and the cognitive function is continuously and generally lower than before, and pathologically, memory is caused by damage and loss of nerve cells. It is a brain disease that causes severe disabilities such as concentration, language, and cognition and progresses gradually over a long period of time, ultimately leading to death. The causes of dementia include Alzheimer's disease, Vascular dementia, Parkinson's disease, Lewy body dementia, Huntington's disease, Creutzfeldt-Jakob disease Jacob disease, and Pick's disease are known.

Alzheimer's disease (AD) is the most common degenerative brain disease and causes cognitive loss due to gradual degeneration of neurons. It is characterized by the accumulation of 39-43 amino acid peptides called amyloid-β protein (Aβ) in fibrillar form, present as amyloid plaques and amyloids in brain tissue. In Alzheimer's disease, Aβ deposition is detrimental to neurons and interstitial cells in the brain, resulting in brain inflammation and neuronal cell death, characteristic characteristics of Alzheimer's disease.

Senile plaque and neurofibrillary tangles in the brain of patients who die from AD appear as pathological features. Among them, senile plaque is formed by accumulation of proteins and dead cells outside the cell, and the main component is a peptide called amyloid-β (Aβ).

The gradual loss of cognitive function, a major characteristic of AD patients, appears to be caused by abnormally accumulated Aβ. Aβ is produced through a proteolysis process from amyloid precursor protein (hereinafter referred to as “APP”). The precursor APP is degraded by β-secretase (BSCE) and γ-secretase to produce Aβ. However, when the APP is cleaved by α-secretase, it inhibits the production of Aβ and induces the production of APPα, which has a neuroprotective effect. Therefore, increasing the activity of α-secretase increases treatment or improvement of Alzheimer's disease. to be. In addition, when SIRT1 is activated in the body, α-secretase is activated by deacetylation of various transcription regulators.

Meanwhile, nicotine receptor inhibitors ABT-418, muscarinic receptor inhibitors xanomerlin, acetylcholine precursor lecithin, acetyl-L-carnitine, metal chelator desperioxamine, rioquinol, beta-sheet breaker iAβ5, iAβ11, Antioxidants vitamin E, ginkgo biloba extract, melatonin, idebenone, sAPP release agents nicotine, acetylcholine, carbacol, beta secretase or gamma secretase inhibitors OM99-1, OM99-2, OM99-3, Z-VLL -CHO, anti-inflammatory drugs such as NSAIDs are being developed, but the effect is still stable enough to mitigate some pathological symptoms or slow the progression. Development is urgent.

Rehmannia glutinosa is a rhizome of Rehmannia glutinosa Libosch., A plant of the Scrophulariaceae family, and its main components are Catalpol, Verbascose mannitol, Glucose, and Mannotriose. (Maninotriose), Vitamin A (Vitamin A). Saengjihwang is cold and tasteless, has no poison, releases all heat, breaks blood, moans, boosts blood and blood, and is used to control hememia.

Red ginseng (紅 蔘) refers to red ginseng dried from the roots of ginseng (水., Panax ginseng C.A. Meyer). The main components of red ginseng are glycosides, ginseng flavor components (panacen), polyacetylene compounds, nitrogen-containing components, flavonoids, vitamins (group B), trace elements, enzymes, antioxidants, organic acids and amino acids, like white ginseng. There is this. It is known that red ginseng has a sedative and excitatory effect on the central nervous system, and acts on the circulatory system to prevent hypertension or arteriosclerosis. In addition, it lowers blood sugar levels (血糖 値), protects the liver, acts on the endocrine system, indirectly acting on sexual and reproductive effects, and has anti-inflammatory and anti-tumor effects.腫 瘍 作用), has a protective effect against radiation and skin protection and softening effect. In addition, the functionality of improving blood flow through fatigue recovery, enhancing immunity, and inhibiting platelet aggregation was recognized.

Baekboknyeong (Poria cocos Wolf) is a mushroom that is parasitic on the roots of pine trees, also known as white solpoongnyeong. It is a potent medicinal herb that strengthens rain, protects the middle and lowers moisture, helps with metabolism, and stabilizes the mind. It grows and grows in places where pine trees from all over the country are cut. It is parasitic on the roots of pine trees that have been cut for many years and grows large like a hump. It is found by stabbing around the stump of a pine tree. It is a lump about 30 ~ 50cm in diameter, rough on the outside like a pine bark, and white inside. It is parasitic on the root of red pine, tastes sweet, bored, and has a flattering effect, diuretic, but not strong. It has been used as a tonic since ancient times. It acts on the menopause, nasal passage, heart, nerve, and bladder mirror to protect the spleen, cut sputum and stabilize the mind. According to the results of pharmacological experiments, it was found that it has a diuretic effect, lowers blood sugar levels, and sedative effects. It is used for cases where the spleen is swelling and the body is swollen, and for damaging diseases. Also, Gew is prescribed for dena, revenge, diarrhea, forgetfulness, and sleep disorders. It is known to have an excellent effect on diseases such as stomach, liver, interest, and kidney, and is used as a medicine for the treatment of people with weakness or chronic gastrointestinal disease after suffering from the disease. It is known to be effective against gynecological diseases.

White wheat (Apis cerana) is a medicinal name for honey that has the effect of adding qi, nourishing the middle, nourishing the lungs, softening the dry, softening the intestines, relieving pain and detoxifying.

Wolfberry (Lycium chinense) is a fruit of the Wolfberry tree belonging to the branch family. Goji berries are round or elliptical berries, and when dried, they are crunchy and contain many seeds inside. The content of goji berry contains 3.39 mg% of Carotene, 0.23 mg% of vitamin B1, 0.33 mg% of vitamin B2, 1.7 mg% of nicotin acid, and 3 mg% of vitamin C, and contains a large amount of various inorganic ingredients such as β-sitosterol and linoleic acid. It is effective in protecting the liver by suppressing and lowering the intestinal absorption of low-specific cholesterol, and is effective in protecting the liver, such as hypertension, angina, arteriosclerosis and diabetes. It is known to have excellent prevention and treatment effects for adult diseases, and also excellent vision protection effects.

Cornus officinalis is a dried fruit of the cornus officinalis Sieb. Et.Zucc, a deciduous small tree belonging to the Cornus family. It is used as an ingredient in Chinese medicine, and in the flesh, glycosides such as cornin, morroniside, loganin, tannin, and saponin, wine acid, malic acid, and tartaric acid It contains organic acids, such as vitamin A and also contains a large amount of sugar. The seeds contain palmitic acid, oleic acid, linoleic acid, and the like. Among the components, cornin is known to have an excitatory action of the parasympathetic nerve. Traditionally, herbal medicine has been used in herbal medicine. According to "Donguibogam", "Hyangyakgyeolseongbang", etc., it is said to have efficacy such as Gangeum, Shinjeong and Singi, and convergence. It is used as a medicine for headaches, tinnitus, sea sickness, fever, and excessive menstruation, and is also used in folk remedies such as cold sweat and enuresis. It is also dressed as tea or alcohol, and has effects such as Jeehan (止汗) and Boeum (補陰).

Omija (Schisandra chinensis) is a ball, about 1 cm in diameter, and is dark red in color. The inside contains 1 to 2 red juice and reddish brown seeds. It has 5 flavors of sweet, sour, bitter, salty, and spicy, and strong acidity among them. Types include Omija (Northern Omija), South Omija, and Black Omija. Contains ingredients such as cyzandrine, gomisin, citral, malic acid, and citric acid, which strengthens the heart, lowers blood pressure, and boosts immunity, making it a tonic. It strengthens the lung function and has antitussive and expectorant properties, which helps to treat cough or thirst. Soak the dried fruit in cold water, add honey and sugar to the red water, drink it as a drink, or make a flower or starch piece to eat. Chestnuts, jujube, and white ginseng are mixed together to make tea or to drink alcohol.

Baekhasuo (Cynanchum wilfordii) is used for nourishment, tonicity, blood, and Ikjeong, and contains lecithin and adrenal cortical hormone substances. It reduces cholesterol, prevents arteriosclerosis, promotes peristalsis, makes bowel movements, stabilizes nervous breakdown, and treats premature ejaculation. Donguibogam cuts inflammation, removes phlegm and phlegm, and treats boils, hemorrhoids, dryness with chronic fatigue, postpartum disease, and treatment.

Prior art related to the present invention is known from the Republic of Korea Patent Publication No. 10-2017-0003395 in a pharmaceutical composition for the prevention and treatment of vascular dementia or Alzheimer's containing pepper extract as an active ingredient, and Republic of Korea Patent Publication 10-2012-0099960 It is known that a composition for preventing and treating dementia using flavonol derived from Hoe plant as an active ingredient. However, until now, it is known about food compositions for preventing or improving immunity or dementia by using 7 kinds of herbal plants and hot water extracts of white wheat, such as raw red bean root, red ginseng root, Baekbokryeong root, goji berry fruit, cornus fruit, Omija fruit, and white sorghum root. It has never been done.

Therefore, the object of the present invention is a food used for preventing or improving immunity or dementia by using 7 kinds of herbal plants and hot water extracts of white wheat, such as raw red bean root, red ginseng root, Baekbokryeong root, goji berry fruit, cornus fruit, Omija fruit, and white sorghum root. In providing a composition.

The above object of the present invention is 20% by weight raw root root, 20% by weight of red ginseng root, 10% by weight of Baekbokryeong root, 10% by weight of white wheat (honey), 10% by weight of goji berry fruit, 10% by weight of cornflower fruit, Omija 10% by weight of the fruit and 10% by weight of white sorghum roots, followed by washing with water and adding purified water of 20 times (w / w) of the total weight of the mixture to a mixture of fine mixtures, followed by extracting hot water with a water heater for 3 hours; Filtering the hot water extract of the herb plant obtained in the above step with two layers of gauze and then concentrating under reduced pressure with a rotary evaporator; It consists of the step of testing the efficacy of the decompressed concentrated hot water extract obtained in the above step through animal experiments and cell experiments, and using the obtained hot water extract as a public disclosure material to perform immune enhancement and dementia prevention and improvement experiments, and the results. It was achieved by evaluation.

In the present invention, 7 kinds of herb plants and white wheat, such as raw huanghuang root, red ginseng root, baekboknyeong root, goji berry fruit, cornus fruit, omija fruit, and white sorghum root, have a weight ratio of 2: 2: 1: 1: 1: 1: 1: 1. It has an effect of providing an extract and has an excellent effect of providing a food composition for preventing or improving immunity or dementia.

Figure 1 is a graph showing the weight change according to the intraperitoneal administration of 8 kinds of hot water extracts, such as raw huanghuang root, red ginseng root, Baekbokryeong root, goji berry fruit, cornus berry, Omija fruit and Baekshou root, according to the present invention.
Figure 2 is a graph showing the DPPH radical scavenging ability of hot water extracts of 8 kinds of herbs, such as saengjihwang root, red ginseng root, Baekbokryeong root, Baekmil (honey), goji berry, cornus fruit, Omija fruit and Baekshao root according to the present invention.
FIG. 3 shows the NO emission amount when the hot water extracts of 8 kinds of medicines, such as raw red bean root, red ginseng root, Baekbokryeong root, white wheat (honey), goji berry, cornus fruit, Omija fruit, and Baeksuo root, according to the present invention are administered to RAW 264.7 cells. It is the graph shown.
Figure 4 is when the administration of hot water extracts of 8 kinds of herbs, such as raw red beans root, red ginseng root, Baekbokryeong root, white wheat (honey), goji berry, cornus fruit, Omija fruit, and white sorghum root according to the present invention to C57BL / 6 mouse spleen cells It is a graph showing B-lymphocyte activity.
FIG. 5 shows macrophage activity when 5xFAD mice were intraperitoneally administered hot water extracts of 8 kinds of herbs, such as saengjihwang root, red ginseng root, Baekbokryeong root, white wheat (honey), goji berries, cornus fruit, Omija fruit, and Baekssuo root according to the present invention. It is the graph shown.
FIG. 6 shows SIRT1 expression level when intraperitoneally administered 5xFAD mice with 8 kinds of hot water extracts, such as saengjihwang root, red ginseng root, Baekbokryeong root, white wheat (honey), goji berry, cornus fruit, Omija fruit, and Baeksuo root, according to the present invention. It is a graph showing.
FIG. 7 shows the Amyloid beta of 8 kinds of hot water extracts, such as saengjihwang root, red ginseng root, Baekbokryeong root, white wheat (honey), goji berry, cornus fruit, Omija fruit, and white sorghum root according to the present invention, intraperitoneally administered to a 5xFAD mouse. It is a graph showing the expression level.
Figure 8 is the amount of expression of sAPPα when administered to HEK293 cells with hot water extracts of 8 medicinal materials, such as saengjihwang root, red ginseng root, Baekbokryeong root, white wheat (honey), goji berry, cornus fruit, Omija fruit and Baekshao root according to the present invention It is a graph showing.
FIG. 9 shows the expression level of sAPPβ when 8 kinds of hot water extracts, such as saengjihwang root, red ginseng root, Baekbokryeong root, white wheat (honey), goji berry, cornus fruit, Omija fruit and Baekshao root, according to the present invention are administered to HEK293 cells. It is a graph showing.

The present invention provides a food composition for preventing and improving dementia.

According to the present invention, the food composition extracts hot water from a mixed herb plant consisting of raw red beans root, red ginseng root, Baekbokryeong root, white wheat (honey), goji berry, cornus berry, Omija fruit, and white sorghum root.

The present invention is explained in more detail by the following specific examples and experimental examples, but the following examples and experimental examples are intended to illustrate the present invention and are not intended to limit the scope of the present invention.

Example 1. Preparation of the hot water extract of the herb plant of 8 kinds of the present invention

Weighing 200 g of raw huanghuang root, 200 g of red ginseng root, 100 g of Baekbokryeong root, 100 g of white wheat (honey), 100 g of goji berry, 100 g of sorghum fruit, 100 g of Omija fruit, and 100 g of white sorghum root are weighed and mixed (Table 1), and then distilled water is used as a total of herbal plants. After adding 20 times the weight (w / w), the hot water was extracted for 3 hours using a gas water heater. After cooling the hot water extract, filtered with two-ply gauze, and concentrated under reduced pressure with a rotary evaporator to obtain 2 kg of concentrate of the hot water extract.

Mixing ratio of raw materials of hot water extract of 8 kinds of medicinal materials of the present invention (% by weight) division designation content One Raw huanghuang root 20 2 Red ginseng root 20 3 Baekbokryeong Root 10 4 White wheat (honey) 10 5 Goji berries 10 6 Cornus officinalis 10 7 Omija fruit 10 8 Baishou Root 10 Total amount 100

Example 2. Preparation of Experimental Animals Experimental animals purchased 7-week-old male 5xFAD mice and C57BL / 6, adapted to a new breeding environment (temperature 22 ± 3 ℃, humidity 50 ± 10%) for one week, and allowed water and feed to be fed freely.

Experimental Example 1. Weight change when administering the hot water extract of 8 kinds of the present invention

To confirm the toxicity of the hot water extract of the eight kinds of the present invention prepared according to Example 1, the experimental animals of Example 2 were administered intraperitoneally at a concentration of 1, 10, 100 mg / mL and the body weight of the experimental animals was measured for one week. To confirm the change. The control group was administered with physiological saline intraperitoneally.

As shown in FIG. 1, it was confirmed that the administration of the hot water extract of the 8 kinds of medicines was not harmful to health because the rapid weight change of the experimental animals did not appear at the concentration of 1 to 100 mg / mL of the hot water extract of the 8 kinds of medicines of the present invention.

Experimental Example 2. Toxicity of hot water extract of 8 kinds of medicine of the present invention

The present invention was measured the toxicity of the hot water extract of the eight kinds of medicine obtained in Example 1 above. To this end, RAW 264.7 cells were dispensed into 24 well plates so as to be 5 x 10 ⁴ cells / well and cultured for 24 hours. The cells were cultured by exchanging the cultured cells with a cell culture solution containing hot water extracts of the 8 kinds of the present invention at concentrations of 1, 10 and 100 mg / mL. After culturing the cells for 48 hours, the MTT assay was performed to dissolve live cell numbers in formazan in isopropanol, and absorbance was measured at a wavelength of 570 nm. As a control group, physiological saline was used.

As a result of the experiment, as shown in Table 2 below, it was confirmed that the hot water extract of the eight kinds of medicines of the present invention is non-toxic and non-toxic.

Saline Eight kinds of herbal extracts (ug / mL) One 10 100 One 1.196 0.987 0.965 0.944 2 1.161 1.061 0.987 0.951 3 1.148 0.971 0.961 0.991 Mean 1.168 1.006 0.971 0.962 % of control 100.0 86.1 83.1 82.3

Experimental Example 3. Measurement of Antioxidant Activity Measuring the antioxidant capacity of hot water extracts of 8 kinds of medicines, such as the present invention obtained from Example 1, roots, red ginseng roots, Baekbokryeong roots, white wheat (honey), goji berry, cornus fruit, Omija fruit, and white sorghum root In order to measure DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity. To this end, 1 mL of 200 uM DPPH and 1 mL of the hot water extract of the eight pharmaceuticals of the present invention obtained in Example 1 were mixed and reacted at room temperature for 30 minutes in a cow, and absorbance was measured at 517 nm. Ascrobic acid was used as the positive control and negative control was stimulated with LPS. DPPH radical scavenging capacity (%) was calculated by the method of Equation 1 below. As a result of the experiment, DPPH radical scavenging ability of the hot water extract of the eight kinds of medicines of the present invention was increased compared to the negative control group, as shown in FIG. Was confirmed.

Experimental Example 4. Immune activity measurement through NO emission

Immunity by measuring NO emission using hot water extracts of 8 kinds of medicines, such as raw gin yellow root, red ginseng root, Baekbokryeong root, white wheat (honey), goji berry, cornus fruit, Omija fruit, and white sorghum root obtained in Example 1 Activity was confirmed. To this end, RAW 264.7 cells were stimulated at a concentration of 100 ng / mL LPS, and then treated with a hot water extract of 8 kinds of the present invention at a concentration of 10 mg / mL to measure the NO release amount. As a control, an LPS-free group was used.

As shown in FIG. 3, it was confirmed that the immune activity was significantly enhanced by releasing a large amount of NO to 74.3% of the hot water extract of 8 medicinal substances at a concentration of 10 mg / mL.

Experimental Example 5. B-lymphocyte stimulation activity measurement

The stimulation activity of B-lymphocytes against the hot water extracts of 8 kinds of medicines, such as the present invention obtained in Example 1, root root, red ginseng root, Baekbokryeong root, white wheat (honey), goji berry fruit, cornus fruit, Omija fruit, and white sewage root To do this, the C57BL / 6 mice described in Example 2 were used for spleen extraction experiments. Splenocytes were cultured in RPMI1640 medium with 10% FBS added, transferred to a 48well plate, stimulated with LPS at a concentration of 25 ug / mL, and then hot water extract of the 8 kinds of medicines of the present invention obtained in Example 1 at a concentration of 10 mg / mL. Treated and cultured for 2 days. The control group was cultured by treatment with physiological saline.

As shown in Fig. 4, it was confirmed that the B-lymphocyte is activated in the hot water extract of the eight kinds of the present invention compared to the control group, thereby significantly enhancing the immune activity.

Experimental Example 6. Measurement of immune activity through macrophage activity

The present invention obtained in Example 1, the raw medicinal root, red ginseng root, Baekbokryeong root, white wheat (honey), goji berry fruit, cornus fruit, Omija fruit, and white sorghum root, etc., the hot water extract of 8 kinds of medicine at the concentration of 100mg / kg Intraperitoneally administered to 5xFAD mice of 2, the control group was administered with physiological saline under the same conditions. The mice were sacrificed 2 days after intraperitoneal administration to obtain macrophage. The obtained macrophages were redispersed in RPMI 1640 medium at a concentration of 1x10 ⁸ pcs / mL, the dispersion was dispensed 200uL, and then cultured in a 5% CO ₂ incubator at 37 ° C for 24 hours, and 0.1% Triton X-100 was added to the cell membrane. The amount of phosphatase of the lysosomal secreted by lysis was measured by absorbance at 405 nm with an ELISA reader, and immunological activity was measured by enhancing macrophage activity.

As shown in FIG. 5, it was confirmed that the activity of the macrophage was increased in the experimental group to which the hot water extract of the eight kinds of the present invention was administered compared to the positive control group, and the immune activity enhancing effect was proved.

Experimental Example 7. SIRT1 level increase measurement

In order to confirm the effect of preventing and improving dementia, the extracts of 8 kinds of medicines, such as the present invention obtained in Example 1, roots of the present invention, red ginseng root, Baekbokryeong root, white wheat (honey), goji berry, cornus fruit, Omija fruit, and white sorghum root The expression level of the SIRT1 gene was confirmed by intraperitoneal administration to the 5xFAD mouse of Example 2 at a concentration of 100 mg / kg. After 2 days of intraperitoneal administration to the mouse, brain tissue was sacrificed and rapidly cooled with liquid nitrogen and used in the experiment. The brain tissue extracted in the above step was quantified by BCA quantification, and 50 ug of protein was subjected to Western blot using 10% SDS-PAGE gel to confirm the expression level of SIRT1. As a negative control, physiological saline was administered at a concentration of 10 uM resveratrol as a positive control.

As shown in FIG. 6, it was confirmed that the hot water extract of the eight kinds of medicinal products of the present invention increased the amount of SIRT1 expression compared to the positive control. As a result, it was confirmed that the dehydration prevention and improvement effect of the hot water extract of the eight kinds of the present invention.

Experimental Example 8. Aβ inhibitory effect measurement

The present invention obtained in Example 1 to determine the effect of preventing and improving dementia by measuring the inhibitory effect of Aβ that causes dementia, red ginseng root, Baekboknyeong root, white wheat (honey), goji berries, cornus fruit, Eight medicinal hot water extracts, such as Omija fruit and white sorghum root, were intraperitoneally administered to the 5xFAD mouse of Example 2 at a concentration of 100 mg / kg, and physiological saline was administered to the control group under the same conditions. Mice were sacrificed 2 days after intraperitoneal administration to obtain blood. Only the supernatant (serum) in the obtained blood was separated and Aβ amount was quantified using the Elisa kit (Thermo fisher).

As shown in Fig. 7, it was confirmed that the production of Aβ was suppressed in the experimental group administered with the hot water extract of the eight kinds of herb plants of the present invention compared to the control group administered with physiological saline. As a result, it was confirmed that the production of Aβ, which is a cause of dementia, was significantly suppressed by the hot water extract of the eight plant plants of the present invention.

Experimental Example 9. Measurement of sAPPα and sAPPβ expression levels

To determine the effect of preventing and improving dementia by measuring the expression levels of sAPPα and sAPPβ, 37 ° C, 5 so that the cells grow 80-90% using DMEM medium with 10% FBS and 1% streptomycin added to HEK293 cells on a plate % CO ₂ Incubated in the incubator, the medium was removed from the culture medium obtained in the above step, and then the cells were recovered with 0.9 mL DMEM medium, and then the present invention obtained in Example 1, raw saenghwang root, red ginseng root, Baekbokryeong root, white wheat (honey), goji berries 8 mL of hot water extracts, such as fruit, cornus fruit, Omija fruit, and white sorghum root, were suspended at a concentration of 10 mg / mL, and 0.1 mL was added and cultured for 24 hours. The control group was used by adding 0.1 mL of DMEM medium instead of the hot water extract of 8 kinds of medicine. The cultures cultured in the above step were subjected to Western blot using 10% SDS-PAGE gel to confirm the expression levels of sAPPα and sAPPβ.

As shown in FIG. 8 and FIG. 9, it was confirmed that the expression level of sAPPα was increased and the expression level of sAPPβ was suppressed in the experimental group to which the hot water extract of the eight kinds of medicines of the present invention was added as compared to the control group. As a result, the expression of the SIRT1 gene was increased by the hot water extract of the eight kinds of the present invention, resulting in increased expression of α-secretase, thereby increasing the expression level of sAPPα and decreasing the expression level of sAPPβ, Aβ causing dementia. It has been demonstrated to significantly inhibit the production of.

In addition, according to the present invention, the present invention included in the food composition containing the hot water extract of 8 kinds of medicines, such as root, red ginseng root, Baekbokryeong root, white wheat (honey), goji berry, cornus fruit, Omija fruit, and white sorghum root as an active ingredient As an edible carrier, excipient or diluent, lactose, dextrose, sucrose, sorbitol, mannitol, starch, acacia rubber, calcium phosphate, alginate, gelatin, calcium silicate, microcrystalline cellulose, polyvinylpyrrolidone , Cellulose, polyvinylpyrrolidone, water, sugar syrup, methylcellulose, methyl hydroxy benzoate, propyl hydroxy benzoate, talc, magnesium stearate and mineral oil were preferred.

In addition, in addition to the above components, a commercially available lubricant, wetting agent, sweetening agent, flavoring agent, emulsifying agent, suspending agent, preservative may be selected to further include any one component.

Hereinafter, a preparation of a health food composition containing 8 types of hot water extracts, such as raw huanghuang root, red ginseng root, baekbokryeong root, white wheat (honey), wolfberry fruit, cornus fruit, omija fruit and white sorghum root, prepared according to the present invention method The specific composition of the will be described.

Preparation Example 1. Preparation of dietary supplements

The present invention obtained in Example 1, the health supplement food containing a concentrate of 8 kinds of hot water extracts, such as raw ginza root, red ginseng root, Baekbokryeong root, white wheat (honey), goji berry, cornus fruit, Omija fruit, and white sorghum root It was prepared according to the conventional method for producing healthy foods with the ingredients in Table 3. This product can be commercialized in formulations such as pills and capsules.

Food ingredient content Material name Content (% by weight) Eight kinds of herbal extract concentrate 75.37 Vitamin A Acetate 0.01 Vitamin E 0.08 Vitamin B1 0.01 Vitamin B2 0.01 Vitamin B6 0.04 Vitamin B12 0.00 Vitamin C 0.75 Biotin 0.00 Nicotinamide 0.13 Folic acid 0.00 Calcium pantothenate 0.04 Ferrous sulfate 0.13 Zinc oxide 0.06 Magnesium carbonate 1.91 Potassium Phosphate 1.13 Dicalcium phosphate 4.15 Potassium citrate 6.78 Calcium carbonate 7.54 Magnesium chloride 1.87 Sum 100.00

Preparation Example 2. Preparation of fermented beverage The beverage obtained from Example 1 of the present invention contains a concentrate of hot water extracts of 8 kinds of medicines, such as the roots of the present invention, red ginseng root, Baekbokryeong root, white wheat (honey), goji berry, cornus fruit, Omija fruit, and white sorghum root. It was prepared according to the conventional beverage production method in the content of Table 4 below. This product was formulated as a pack formulation.

Beverage ingredient content Material name Content (% by weight) Eight kinds of herbal extract concentrate 10.0 Liquid fructose (wt%) 12.0 Dextrin (wt%) 0.5 Citric acid (wt%) 0.13 Calcium lactate (wt%) 1.6 Vitamin C (wt%) 0.03 Trisodium citrate (wt%) 0.02 Gel Tan Gum (wt%) 0.03 Purified water (wt%) Balance Total (wt%) 100.0

As described above, the hot water extracts of 8 kinds of medicines, such as the present invention raw root, red ginseng root, Baekbokryeong root, white wheat (honey), goji berry, cornus fruit, Omija fruit, and white sorghum root, are excellent in improving immunity and preventing and improving dementia. Because it is effective, it is a very useful invention in the health functional food industry.

Claims (3)

Raw Ginseng Root, Red Ginseng Root, Baekbokryeong Root, White Wheat, Goji Fruit, Cornus officinalis, Omija Fruit, and White Sewage Root in a powder obtained by fine-graining 8 kinds of herbs consisting of 2: 2: 1: 1: 1: 1: 1: 1 Preparing a hydrothermal extract concentrate by adding 20 times (w / w) purified water by adding water, extracting hot water for 3 hours, filtering with 2-ply gauze after cooling, and then concentrating under reduced pressure with a rotary evaporator; And
Prepared hot water extract concentrate as a carrier, excipient or diluent, lactose, dextrose, sucrose, sorbitol, mannitol, starch, acacia rubber, calcium phosphate, alginate, gelatin, calcium silicate, polyvinylpyrrolidone, cellulose, water , Sugar syrup, methylcellulose, methyl hydroxy benzoate, propyl hydroxy benzoate, talc, magnesium stearate and any additive selected from mineral oils to be mixed with any additive selected to prepare a food composition containing a hot water extract as an active ingredient Characterized in that it comprises a;
Method for preparing food composition for preventing and improving dementia that suppresses the production of amyloid-beta. delete According to claim 1,
The step of preparing the food composition is characterized by further mixing calcium lactate and gelatin.
Method for preparing food composition for preventing and improving dementia that suppresses the production of amyloid-beta.

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