KR102088381B1 - 압타머-기반 다중 검정법 - Google Patents

압타머-기반 다중 검정법 Download PDF

Info

Publication number
KR102088381B1
KR102088381B1 KR1020147034387A KR20147034387A KR102088381B1 KR 102088381 B1 KR102088381 B1 KR 102088381B1 KR 1020147034387 A KR1020147034387 A KR 1020147034387A KR 20147034387 A KR20147034387 A KR 20147034387A KR 102088381 B1 KR102088381 B1 KR 102088381B1
Authority
KR
South Korea
Prior art keywords
aptamer
delete delete
target
solid support
tag
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
KR1020147034387A
Other languages
English (en)
Korean (ko)
Other versions
KR20150030655A (ko
Inventor
글렌 샌더스
스테판 크레이머
에벌더스 캐틸리어스
올리바스 에드가
Original Assignee
소마로직, 인크.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 소마로직, 인크. filed Critical 소마로직, 인크.
Publication of KR20150030655A publication Critical patent/KR20150030655A/ko
Application granted granted Critical
Publication of KR102088381B1 publication Critical patent/KR102088381B1/ko
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/205—Aptamer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2570/00—Omics, e.g. proteomics, glycomics or lipidomics; Methods of analysis focusing on the entire complement of classes of biological molecules or subsets thereof, i.e. focusing on proteomes, glycomes or lipidomes

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
KR1020147034387A 2012-06-07 2013-06-07 압타머-기반 다중 검정법 Active KR102088381B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261656956P 2012-06-07 2012-06-07
US61/656,956 2012-06-07
PCT/US2013/044792 WO2013185078A1 (en) 2012-06-07 2013-06-07 Aptamer-based multiplexed assays

Publications (2)

Publication Number Publication Date
KR20150030655A KR20150030655A (ko) 2015-03-20
KR102088381B1 true KR102088381B1 (ko) 2020-03-13

Family

ID=49712701

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020147034387A Active KR102088381B1 (ko) 2012-06-07 2013-06-07 압타머-기반 다중 검정법

Country Status (21)

Country Link
US (5) US20150141259A1 (https=)
EP (1) EP2859121B1 (https=)
JP (1) JP6298456B2 (https=)
KR (1) KR102088381B1 (https=)
CN (2) CN104508150B (https=)
AU (1) AU2013271401B2 (https=)
BR (1) BR112014030298B1 (https=)
CA (1) CA2873099C (https=)
CY (1) CY1118748T1 (https=)
ES (1) ES2617226T3 (https=)
HR (1) HRP20170296T1 (https=)
IL (1) IL235588B (https=)
IN (1) IN2014KN02647A (https=)
MX (1) MX356413B (https=)
MY (1) MY170164A (https=)
NZ (1) NZ701824A (https=)
PH (1) PH12014502581B1 (https=)
RU (1) RU2666989C2 (https=)
SG (2) SG10201701361WA (https=)
WO (1) WO2013185078A1 (https=)
ZA (1) ZA201408409B (https=)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8975026B2 (en) 2007-01-16 2015-03-10 Somalogic, Inc. Method for generating aptamers with improved off-rates
US20110136099A1 (en) 2007-01-16 2011-06-09 Somalogic, Inc. Multiplexed Analyses of Test Samples
US7947447B2 (en) 2007-01-16 2011-05-24 Somalogic, Inc. Method for generating aptamers with improved off-rates
WO2015109020A1 (en) * 2014-01-14 2015-07-23 Aptitude Medical Systems, Inc. Use of nucleic acid agents for ultra-sensitive digital detection and quantification of target molecules
CA3152415A1 (en) 2014-02-18 2015-08-27 Somalogic Operating Co., Inc. Compositions and methods for detecting microorganisms
CN106574925B (zh) * 2014-05-23 2019-07-26 萤火虫生物股份有限公司 用于生物测定的底物介导的反应器
GB2528643A (en) * 2014-07-08 2016-02-03 Adc Biotechnology Ltd Method of synthesising ADCs
KR102003589B1 (ko) * 2016-03-03 2019-07-24 성균관대학교산학협력단 생화학 분자 검출센서 및 다파장 형광을 이용한 생화학 분자 검출방법
CN105779609A (zh) * 2016-04-15 2016-07-20 上海伊丽萨生物科技有限公司 一种超敏免疫检测方法
CA3039057A1 (en) * 2016-10-13 2018-04-19 Laboratory Corporation Of America Holdings Devices and methods to reduce interfering compounds in biological samples
CN120992918A (zh) * 2018-04-13 2025-11-21 富鲁达加拿大公司 用于元素分析的稳定细胞采集
EP3810563A4 (en) * 2018-06-22 2022-03-23 Somalogic, Inc. IMPROVED PROTEOMIC MULTIPLEX TESTS
US11181501B2 (en) 2018-09-11 2021-11-23 City University Of Hong Kong Method and an apparatus for determining a presence or an amount of a polyamine or its derivative in a sample
LU101073B1 (en) 2018-12-21 2020-06-24 Luxembourg Inst Science & Tech List Dna aptamers specific of adenovirus types
KR102177672B1 (ko) * 2019-02-01 2020-11-12 주식회사 압타머사이언스 압타머를 이용한 다중(multiplex) PCR 방법
KR102113078B1 (ko) * 2019-07-05 2020-05-20 광주과학기술원 압타머의 선별 방법
CN110791551A (zh) * 2019-09-17 2020-02-14 北京化工大学 一种无载体氯霉素适配体信号放大传感器的建立方法
KR102403628B1 (ko) * 2019-12-03 2022-05-30 주식회사 바이오이즈 시료의 표적분자 집단에 대한 프로파일을 얻는 방법
CN113624724A (zh) * 2020-05-07 2021-11-09 廖世奇 一种适配体分子信标对靶分子的多元检测分析方法
CN111812313B (zh) * 2020-06-22 2021-10-08 北京生物制品研究所有限责任公司 铝佐剂吸附型新型冠状病毒灭活疫苗中抗原的解离方法
WO2022232308A1 (en) 2021-04-27 2022-11-03 Singular Genomics Systems, Inc. High density sequencing and multiplexed priming
WO2022265362A1 (ko) * 2021-06-14 2022-12-22 김성천 압타머를 이용하여 시료의 표적 단백질 분자 집단에 대한 프로파일을 얻는 방법
EP4457358A1 (en) * 2021-12-30 2024-11-06 SomaLogic Operating Co., Inc. Next-generation sequencing for protein measurement
CN116103367B (zh) * 2022-11-11 2025-12-23 杭州佰辰医学检验所有限公司 一种检测靶标物的方法
WO2025064457A1 (en) * 2023-09-19 2025-03-27 Somalogic Operating Co., Inc. Methods of solid-phase nucleic acid hybridization
WO2025166038A1 (en) * 2024-02-02 2025-08-07 Illumina, Inc. Improved aptamer detection techniques
CN121272015A (zh) * 2024-07-03 2026-01-06 常州福洛森医疗科技有限公司 适配体在可寻址性检测中应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004009798A2 (en) 2002-07-24 2004-01-29 Ciphergen Biosystems, Inc. Protein interaction difference mapping

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0786469B1 (en) 1990-06-11 2006-03-01 Gilead Sciences, Inc. Methods of use of nucleic acid ligands
US6242246B1 (en) 1997-12-15 2001-06-05 Somalogic, Inc. Nucleic acid ligand diagnostic Biochip
PT1232502E (pt) * 1999-11-17 2006-05-31 Roche Diagnostics Gmbh Particulas de vidro magneticas, metodo para a sua preparacao e suas utilizacoes
US7601497B2 (en) * 2000-06-15 2009-10-13 Qiagen Gaithersburg, Inc. Detection of nucleic acids by target-specific hybrid capture method
KR101168654B1 (ko) * 2004-05-19 2012-07-25 브이피 호울딩 엘엘씨 표면 증강 라만 산란에 의한 화학기의 증강된 검출을 위한 층상의 플라즈몬 구조를 가진 광센서
US7699979B2 (en) * 2005-01-07 2010-04-20 Board Of Trustees Of The University Of Arkansas Separation system and efficient capture of contaminants using magnetic nanoparticles
US7285835B2 (en) * 2005-02-24 2007-10-23 Freescale Semiconductor, Inc. Low power magnetoelectronic device structures utilizing enhanced permeability materials
US8637656B2 (en) * 2005-07-05 2014-01-28 Ribomic Inc. Nucleic acid capable of binding to immunoglobulin G and use thereof
WO2007084886A2 (en) * 2006-01-17 2007-07-26 Somalogic, Incorporated Multiplexed analyses of test samples
US7947447B2 (en) * 2007-01-16 2011-05-24 Somalogic, Inc. Method for generating aptamers with improved off-rates
US7855054B2 (en) * 2007-01-16 2010-12-21 Somalogic, Inc. Multiplexed analyses of test samples
US7964356B2 (en) 2007-01-16 2011-06-21 Somalogic, Inc. Method for generating aptamers with improved off-rates
DK2172566T4 (da) * 2007-07-17 2022-06-13 Somalogic Inc Fremgangsmåde til generering af aptamerer med forbedrede off-rates
US8291501B2 (en) * 2008-02-08 2012-10-16 Cheng Holdings, Llc Validation of protected intra-system interconnects for digital rights management in electrical computers and digital data processing systems
CN102046138A (zh) * 2008-05-30 2011-05-04 欧莱雅 头发处理方法及成套用品
AU2009273052B2 (en) * 2008-07-14 2015-09-17 The University Of Tokyo Aptamer against IL-17 and use thereof
EP2418968B1 (en) * 2009-04-13 2015-12-02 Agriculture and Food Development Authority (TEAGASC) Method of producing microbeads
GB0916700D0 (en) * 2009-09-23 2009-11-04 Nanoco Technologies Ltd Semiconductor nanoparticle-based materials

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004009798A2 (en) 2002-07-24 2004-01-29 Ciphergen Biosystems, Inc. Protein interaction difference mapping

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
PLOS ONE, VOl.5., No.12., page e15004 (2010)

Also Published As

Publication number Publication date
MX356413B (es) 2018-05-29
RU2014152698A (ru) 2016-08-10
US20170176422A1 (en) 2017-06-22
BR112014030298B1 (pt) 2022-09-27
SG11201407505XA (en) 2014-12-30
IL235588A0 (en) 2015-01-29
BR112014030298A2 (pt) 2017-06-27
US20180224444A1 (en) 2018-08-09
US20150141259A1 (en) 2015-05-21
EP2859121A4 (en) 2015-12-16
HK1204341A1 (en) 2015-11-13
CA2873099A1 (en) 2013-12-12
CN104508150A (zh) 2015-04-08
PH12014502581B1 (en) 2022-02-02
CN104508150B (zh) 2021-03-12
ES2617226T3 (es) 2017-06-15
KR20150030655A (ko) 2015-03-20
IN2014KN02647A (https=) 2015-05-08
US20250147022A1 (en) 2025-05-08
PH12014502581A1 (en) 2015-01-21
ES2617226T8 (es) 2019-08-27
IL235588B (en) 2018-07-31
NZ701824A (en) 2017-01-27
EP2859121A1 (en) 2015-04-15
AU2013271401A1 (en) 2014-11-27
US20210239692A1 (en) 2021-08-05
MY170164A (en) 2019-07-09
WO2013185078A1 (en) 2013-12-12
JP2015520385A (ja) 2015-07-16
CY1118748T1 (el) 2017-07-12
RU2666989C2 (ru) 2018-09-13
CN108614102A (zh) 2018-10-02
ZA201408409B (en) 2017-08-30
SG10201701361WA (en) 2017-04-27
EP2859121B1 (en) 2016-11-30
MX2014014784A (es) 2015-03-19
AU2013271401B2 (en) 2018-04-05
JP6298456B2 (ja) 2018-03-20
HRP20170296T1 (hr) 2017-04-21
BR112014030298A8 (pt) 2022-03-08
CA2873099C (en) 2021-06-15

Similar Documents

Publication Publication Date Title
KR102088381B1 (ko) 압타머-기반 다중 검정법
US9404919B2 (en) Multiplexed analyses of test samples
US7855054B2 (en) Multiplexed analyses of test samples
AU2008275917B2 (en) Multiplexed analyses of test samples
AU2019290130B2 (en) Improved proteomic multiplex assays
AU2017202493B2 (en) Multiplexed analyses of test samples
CA3104041C (en) Improved proteomic multiplex assays
HK1260876A1 (en) Aptamer-based multiplexed assays
RU2792385C2 (ru) Усовершенствованные протеомные мультиплексные анализы
HK1204341B (en) Aptamer-based multiplexed assays
AU2013203360B2 (en) Multiplexed analyses of test samples

Legal Events

Date Code Title Description
PA0105 International application

St.27 status event code: A-0-1-A10-A15-nap-PA0105

P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

P13-X000 Application amended

St.27 status event code: A-2-2-P10-P13-nap-X000

PG1501 Laying open of application

St.27 status event code: A-1-1-Q10-Q12-nap-PG1501

R17-X000 Change to representative recorded

St.27 status event code: A-3-3-R10-R17-oth-X000

A201 Request for examination
E13-X000 Pre-grant limitation requested

St.27 status event code: A-2-3-E10-E13-lim-X000

P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

P13-X000 Application amended

St.27 status event code: A-2-2-P10-P13-nap-X000

PA0201 Request for examination

St.27 status event code: A-1-2-D10-D11-exm-PA0201

P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

P13-X000 Application amended

St.27 status event code: A-2-2-P10-P13-nap-X000

R15-X000 Change to inventor requested

St.27 status event code: A-3-3-R10-R15-oth-X000

R16-X000 Change to inventor recorded

St.27 status event code: A-3-3-R10-R16-oth-X000

P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

P13-X000 Application amended

St.27 status event code: A-2-2-P10-P13-nap-X000

E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

St.27 status event code: A-1-2-D10-D21-exm-PE0902

P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

P13-X000 Application amended

St.27 status event code: A-2-2-P10-P13-nap-X000

E701 Decision to grant or registration of patent right
PE0701 Decision of registration

St.27 status event code: A-1-2-D10-D22-exm-PE0701

GRNT Written decision to grant
PR0701 Registration of establishment

St.27 status event code: A-2-4-F10-F11-exm-PR0701

PR1002 Payment of registration fee

St.27 status event code: A-2-2-U10-U12-oth-PR1002

Fee payment year number: 1

PG1601 Publication of registration

St.27 status event code: A-4-4-Q10-Q13-nap-PG1601

PN2301 Change of applicant

St.27 status event code: A-5-5-R10-R13-asn-PN2301

St.27 status event code: A-5-5-R10-R11-asn-PN2301

PR1001 Payment of annual fee

St.27 status event code: A-4-4-U10-U11-oth-PR1001

Fee payment year number: 4

PR1001 Payment of annual fee

St.27 status event code: A-4-4-U10-U11-oth-PR1001

Fee payment year number: 5

PR1001 Payment of annual fee

St.27 status event code: A-4-4-U10-U11-oth-PR1001

Fee payment year number: 6

PR1001 Payment of annual fee

St.27 status event code: A-4-4-U10-U11-oth-PR1001

Fee payment year number: 7

U11 Full renewal or maintenance fee paid

Free format text: ST27 STATUS EVENT CODE: A-4-4-U10-U11-OTH-PR1001 (AS PROVIDED BY THE NATIONAL OFFICE)

Year of fee payment: 7